WO2019033835A1

WO2019033835A1 - Medicament for effectively repelling adult female bactroceraminax (enderlein) and use thereof

Info

Publication number: WO2019033835A1
Application number: PCT/CN2018/090294
Authority: WO
Inventors: 欧阳革成; 侯柏华; 卢慧林; 方小端; 孟翔
Original assignee: 广东省生物资源应用研究所
Priority date: 2017-08-15
Filing date: 2018-06-07
Publication date: 2019-02-21
Also published as: AU2018317246B2; AU2018317246A1; CN107494536A; CN107494536B

Abstract

Disclosed are a medicament for effectively repelling adult female Bactroceraminax (Enderlein) and the use thereof. The combined use of methyl eugenol and cuelure has a significant repellent effect on adult female Bactroceraminax (Enderlein), and therefore, a combination of methyl eugenol and cuelure can be used as a repellent for adult female Bactroceraminax (Enderlein).

Description

Medicament and application thereof for effectively repelling adult female larvae of citrus

Technical field:

The invention belongs to the field of citrus fruit fly control, and particularly relates to a medicament which can effectively repel female adults of the citrus fruit fly and its application.

Background technique:

Bactroceraminax (Enderlein) is one of the important pests of citrus in China. It is seriously endangered in citrus gardens in Hunan, Hubei, Chongqing and Sichuan provinces. After the adults of the fruit fly have spawned fruit, there will be no remedial measures, which will cause the citrus fruit to yellow and fall off in advance. The fruit that has not fallen off loses the commodity value because its larva is harmful in the fruit, causing a large reduction in production when it occurs, giving the fruit farmers Come to serious economic losses. At present, there are many researches on the prevention and control of the citrus fruit fly, mainly through the early harvesting of the fruit with obvious fruit, the yellow fruit picked up by the larvae at any time, the fruit is thoroughly picked up, and the pit is deeply buried, boiled, and burnt. Wait until the purpose of killing eggs and removing larvae. At the same time, the use of bait (sweet vinegar or flies), trapping the ball to induce adult insects is also a key technology to prevent and control large fruit fly. However, these trapping techniques are costly and are used in orchards, which tend to lure fruit flies elsewhere. If you do not unify large-area applications, users will not be able to pay for it.

Methyl eugenol is a chemical attractant chemical information substance currently used for field monitoring and control of Bactrocera dorsalis, and its attracting effect on male adults is very good. The fly ketone is also a relatively effective attractant of the fruit fly, and has certain attracting activity on 56 closely related species such as Queensland fruit fly and small pumpkin fruit fly.

Summary of the invention:

A first object of the present invention is to provide an agent which is effective for repelling female adults of the citrus fruit fly.

The medicament of the present invention which is effective for repelling adult females of Bactrocera dorsalis, characterized in that it comprises methyl eugenol and gamma.

A second object of the present invention is to provide the use of the above-mentioned agent which is effective for repelling female adults of Bactrocera dorsalis in the preparation of a female adult repellent for Bactrocera dorsalis.

A third object of the present invention is to provide an application of methyl eugenol combined with gamma in the preparation of a female adult repellent for Bactrocera dorsalis.

The mass ratio of the methyl eugenol to the gamma is 5:2.

The present invention finds that the combination of methyl eugenol and fly ketone has a significant repellent effect on the female adult larva of the citrus fruit fly, so the combination of methyl eugenol and gamma can be used as a repellent for female adults of the genus Bactrocera , to provide new ideas for the comprehensive prevention and control of citrus fruit fly.

BRIEF DESCRIPTION OF THE DRAWINGS:

Figure 1 is a comparative analysis of the total number of insects in a single green ball in A and B gardens.

Detailed ways:

The following examples are intended to further illustrate the invention and not to limit the invention.

Example 1:

1 test material

The green sticky ball simulating the citrus fruit was provided by the Hunan Horticultural Research Institute; methyl eugenol (ME) and fly ketone were purchased from Guangzhou Ruifeng Biotechnology Co., Ltd.;

2 test methods

The experiment was carried out in a citrus garden in Anjiang Town, Huaihua City, Hunan Province. Select two citrus orchard A and B gardens of the same age and growth. The distance between them is 600m and the area is about 1 mu. On May 20, 2017, 9 green sticky balls with a diameter of 7cm are hung on the citrus tree in the A garden. There are 7 green sticky balls hanging in the B garden, and one tree is hung. On June 9th, the number of citrus fruit flies on each ball in the two parks was first examined and the male and female were identified. And put the same amount of green sticky balls in the same place, and at the same time, put three sets of combined attractants on the citrus tree in the A garden. Each group of attracting agents is to put two small attracting bottles in a large attracting bottle. One small attracting bottle was added with 5 mg ME, and the other small attracting bottle was added with 2 mg of fly gamma. Each group of combined attractants was 10 m apart. B garden is not hanging. After 10 days, the number of citrus fruit flies on the sticky ball was checked for the second time, and the male and female were identified.

3 test results

The results of the investigation of the green ball in the citrus australis A and B gardens to attract the citrus fruit fly are shown in Tables 1, 2, 3 and Figure 1. The results showed that in the A garden, the results of two times before and after the combination of attracting the attractant showed that the female rate of the fruit fly, the average number of attracting insects per day, and the average number of females per day were There was a significant difference, and the combination of attractants was not administered in B Park. No difference was found in the previous two surveys. In addition, there was no significant difference between the A and B gardens for the first time to induce the female sex rate of the fruit fly, but after the combination of the attractant in the A garden, the difference was significant. Looking at the total number of citrus larvae from the green ball (Fig. 1), after the combination of the attractant, the number of A gardens was greatly reduced, and the B garden was significantly increased.

From the above experimental results, it can be seen that after the combination of the attractant, the attracting effect of the green ball on the adult female larvae of the citrus fly is weakened, indicating that it has a better repellent effect on the female adult, and can be used as a repellent in the field. Effective control of citrus fruit fly.

Table 1 Comparative analysis of the female rate of the green globus in the two parks A and B

Note: The P value is the mean value of the independent sample T test (two-tailed test).

Table 2 Comparative analysis of the average daily number of insects per ball in the green balls of A and B in the two parks

Table 3 Comparison of the average daily females per ball in the A and B gardens

Claims

An agent effective for repelling adult females of Bactrocera dorsalis, characterized by comprising methyl eugenol and gamma.
The medicament according to claim 1, wherein the mass ratio of the methyl eugenol to the gamma is 5:2.
The use of the agent according to claim 1 for effectively repelling female adults of Bactrocera dorsalis in the preparation of a female adult repellent for Bactrocera dorsalis.
The use according to Claim 3, characterized in that the mass ratio of methyl eugenol to gamma is 5:2.
The application of methyl eugenol combined with fly ketone in the preparation of female adult repellent of Bactrocera dorsalis.
The use according to Claim 5, characterized in that the mass ratio of methyl eugenol to gamma is 5:2.