CN108902199B - Application of eucalyptus essential oil in attracting bactrocera minax - Google Patents

Application of eucalyptus essential oil in attracting bactrocera minax Download PDF

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CN108902199B
CN108902199B CN201810946562.3A CN201810946562A CN108902199B CN 108902199 B CN108902199 B CN 108902199B CN 201810946562 A CN201810946562 A CN 201810946562A CN 108902199 B CN108902199 B CN 108902199B
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essential oil
trapping
eucalyptus
killing
bactrocera minax
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CN108902199A (en
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龚碧涯
刘慧�
肖伏莲
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Hunan Institute Of Horticulture
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00—Stationary means for catching or killing insects
    • A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • A01M1/04—Attracting insects by using illumination or colours
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove

Abstract

The invention discloses application of eucalyptus essential oil in attracting Bactrocera minax, wherein the eucalyptus essential oil has a good attracting effect on male and female imagoes of Bactrocera minax. The eucalyptus citriodora essential oil can be directly developed into a high-efficiency attractant or can be compounded with other attractants to be applied to the prevention and control of bactrocera minax imagoes, and a new idea can be provided for the research and development of new attractant compounds.

Description

Application of eucalyptus essential oil in attracting bactrocera minax
Technical Field
The invention belongs to the field of pest control, and particularly relates to application of eucalyptus essential oil in luring bactrocera minax.
Background
The bactrocera minax is a destructive pest on citrus, and is mainly harmful to the fact that adults lay eggs in young fruits, the eggs are hatched into young insects which eat citrus fruits, and the larvae pupate in soil after the fruits emerge, so that the prevention and the control are difficult. The most common control method of bactrocera minax is adult trapping and killing technology, which mainly comprises trapping and killing by a attractant and trapping and killing by a trap. The commonly used trapping and killing agents are from the simplest sugar-vinegar liquid to slightly complex yeast hydrolyzed protein, orange juice and orange peel essential oil are added into the sugar-vinegar liquid and the yeast hydrolyzed protein at present, the trapping and killing agents belong to food trapping agents and can trap Bactrocera minax to a certain extent, but the trapping and killing effects of the trapping and killing agents are poor compared with those of a viscose type trapping and killing device, and the trapping and killing agents are gradually replaced by the viscose type trapping and killing device.
The attractant and the trap can be combined with each other, but because the food attractant can attract and kill the bactrocera minax and other non-target insects, the combination of the food attractant and the viscose trap can increase the non-target insects on the trap and reduce the using effect of the trap and the trap. At present, patents related to the field of bactrocera minax attractants include food attractants and pheromone attractants, an improved sugar-vinegar liquid formula is invented by Zhao Shi et al (2006), the improved sugar-vinegar liquid formula is a better food attractant for early prevention and control of bactrocera minax, a protein attractant is obtained by adding yeast hydrolyzed protein into long leaves of cattle and the like (2010), and Hansenula anomala is also found to be used as the bactrocera minax attractants by Zhang Hongyu and the like (2013). Sex pheromone is a high-efficiency attractant component, and 4 reports about the sex pheromone of Bactrocera minax are reported, Lu academic Red et al (1998) extract a sex pheromone crude extract, but do not clearly define active ingredients, and Xia Zhong Ying extract sex pheromone 2, 6-di-tert-butyl-p-cresol, but the application effect in the field is not good. The sex pheromone secreted by the female adult is found to be undecanol by the setron, indoor tests show that the sex pheromone has an attraction effect on the male adult, Zhang Hongyu also collects volatile substances released by the female adult of the bactrocera minax through headspace adsorption, and separates the volatile substances to obtain R- (+) -limonene.
Disclosure of Invention
The invention aims to provide a new application of eucalyptus essential oil, in particular to eucalyptus citriodora essential oil, namely the application of the eucalyptus citriodora essential oil in a bactrocera minax imago attractant, which has a good attraction effect on both male and female bactrocera minax imagoes.
The invention provides application of eucalyptus essential oil in luring bactrocera minax. The Bactrocera minax refers to adult Bactrocera minax.
The eucalyptus essential oil is eucalyptus citriodora essential oil or eucalyptus globulus essential oil.
Preferably, liquid paraffin and/or n-hexane are/is used as a solvent, and 1mL of eucalyptus essential oil is dissolved in 6-20mL of the solvent to be used as the attractant for the bactrocera minax.
Preferably, green balls with the diameter of 5-10cm are used as the trapping and killing balls for the Bactrocera minax, and the trapping core is placed in the trapping and killing balls.
Preferably, the yellow-green bicolor ball with the diameter of 5-10cm is used as a trapping ball for the bactrocera minax, and the trapping core is placed in the trapping ball.
The eucalyptus essential oil is a plant essential oil obtained by steaming and extracting leaves of lemon eucalyptus or blue eucalyptus through a rotary evaporator.
The using concentration of the eucalyptus citriodora essential oil is 8-20%.
Preferably, the diameter of the killer ball is 6.5-8.5cm, more preferably 7 cm.
As an improvement of the technical scheme, the application concentration of the eucalyptus citriodora essential oil applied to the trapping and killing of the bactrocera minax imagoes is 10%, the used solvent is liquid paraffin or n-hexane, namely 1ml of the eucalyptus citriodora essential oil is added into every 9ml of the liquid paraffin or n-hexane solvent.
The eucalyptus citriodora essential oil is a plant essential oil extracted from eucalyptus, and mainly comprises citronellal, rhodinol, citric alcohol and the like. The reports show that the eucalyptus citriodora essential oil has a repellent effect on termites, borers, red flour beetles, mulberryn, yellow star mulberryn, monochamus alternatus adults and the like, and is usually used as a mosquito and fly repellent in the market, but the application of the eucalyptus citriodora essential oil to the bactrocera minax and the attractant for the bactrocera minax adults is not provided.
The invention provides a new application of eucalyptus citriodora essential oil in a pioneering way, which is realized by the following technical scheme:
the behavior reaction of the bactrocera minax adults to different essential oils is tested by the applicant by adopting a Y-type olfactometer, and the use concentration of the different essential oils is 10 percent.
And b, comparing the selection rates of the bactrocera minax adults on different essential oils, so that the selection rate of the bactrocera minax adults on the eucalyptus citriodora essential oil is the highest, and the selection rates of the bactrocera minax female and male adults on the eucalyptus citriodora essential oil are both 50%.
c, preparing the eucalyptus citriodora essential oil into a trapping core, suspending the trapping core together with the 7cm green trapping and killing ball, and measuring the trapping effect in the field, so that the effect of trapping the 7cm green trapping and killing ball can be remarkably improved by the eucalyptus citriodora essential oil, and the comprehensive synergistic effect is 31.78%.
d, the luring core prepared by the eucalyptus citriodora essential oil and the existing luring agents of limonene, a sugar-vinegar solution, a yeast hydrolyzed protein and fructide are respectively placed in a 7cm green luring and killing ball for field luring effect measurement, and the luring core prepared by the eucalyptus citriodora essential oil and the luring and killing ball are proved to have a better luring and killing effect on the bactrocera minax than the luring and killing ball prepared by the limonene and the luring and killing ball, and the luring and killing effect on the bactrocera minax is lower than that of the luring and killing ball prepared by the single use of the sugar-vinegar solution, the yeast hydrolyzed protein, the fructide and the luring and killing ball prepared by the eucalyptus citriodora essential oil and the luring and killing ball prepared by the single use.
The new application of the eucalyptus essential oil provided by the invention can directly develop the eucalyptus citriodora essential oil into a high-efficiency attractant, or can be compounded with other attractants to be applied to the control of bactrocera minax imagoes, and can also provide a new idea for researching and developing new attractant compounds. The invention can also be used together with the trapping and killing ball, thereby reducing the number of other non-target insects on the trapping and killing ball and improving the trapping and killing effect of the trapping and killing ball. The invention has no harm to natural enemies, people, livestock and crops, and has the advantages of environmental protection and the like.
Detailed Description
The present invention is further illustrated by the following specific examples, which should not be construed as limiting the scope of the invention.
Example 1:
the application of the eucalyptus citriodora essential oil in the bactrocera minax attractant is discovered through the following researches:
a. and (3) measuring the behavior reaction of the male and female citrus fruit flies to different essential oils.
The different essential oils are eucalyptus citriodora essential oil, peppermint essential oil, neroli essential oil, orange leaf essential oil, wormwood essential oil, gardenia essential oil, orange essential oil, grapefruit essential oil and limonene and are diluted by liquid paraffin, namely the essential oils and the liquid paraffin are diluted according to the proportion of 1: 10, mixing;
the testing method comprises the following steps: adding 1ml of essential oil diluent into one arm of the Y-shaped pipe, adding an equal amount of liquid paraffin oil into the other arm, testing 20-30 adult insects for each essential oil, putting 1 test insect from a main pipe port each time, recording the time of the test insects staying in each area and the times of entering each arm within 5min, recording that the test insects do not enter two branch arms within 5min and recording that the test insects do not react, reversing the Y-shaped pipe after testing 5 test insects, and wiping the inner wall of the Y-shaped pipe with absolute ethyl alcohol after testing one essential oil.
b. Calculating the selection rate of the male and female citrus fruit flies to different essential oils
According to the times of the test insects entering each arm, the selection rates of the female citrus fruit fly and the male citrus fruit fly to each essential oil are respectively calculated according to the total number of the test insects and the number of the test insects on each arm, the influence of each essential oil on the selection of the female citrus fruit fly and the male citrus fruit fly is analyzed, the specific results are shown in attached table 1, and the selection rates of the female citrus fruit fly and the male citrus fruit fly to the eucalyptus citriodora essential oil are both 50 percent and are far higher than the selection rates of other essential oils and are also higher than limonene (attractant components invented by Zhang Hongyu and the like) from the attached table 1.
TABLE 1 tendency of Bactrocera minax adults to different essential oils
Figure BDA0001770370430000031
c. The field verifies the luring effect of the eucalyptus citriodora essential oil
Diluting the Eucalyptus citriodora essential oil with liquid paraffin oil to 10% concentration, making into lure with non-woven cotton piece with radius of 1.5cm as carrier, and adding diluted Eucalyptus citriodora essential oil 5ml into each non-woven cotton piece. In the last ten days of 6 months, the trapping cores are placed in green trapping and killing balls with the diameter of 7cm, wherein CK is the trapping and killing balls with the diameter of 7cm, no trapping core is added, odor release holes are formed in the bottoms of the trapping and killing balls with the diameter of 7cm, the trapping and killing balls are hung in the field, 4 blocks are arranged for each treatment, 5 repeats are arranged in each block, the number of bactrocera minax imagoes on the trapping and killing balls with the trapping cores and without the trapping cores is observed, the number of the bactrocera minax imagoes on the trapping and killing balls with the trapping cores is higher than that of the bactrocera minax imagoes on the trapping and killing balls without the trapping cores, the trapping and killing effect of the trapping and killing balls can be improved by about 31% by using the eucalyptus citriodora essential oil, and the result proves that the eucalyptus citriodora oil has a remarkable trapping and killing effect on the bactrocera mina.
Ephempseed 2 influence of Eucalyptus citriodora essential oil lure on the luring effect of green luring and killing ball with diameter of 7cm
Figure BDA0001770370430000041
Note: the data in the table are the number of bactrocera minax on the trapping and killing ball. Wherein, treatment 1 is the lure and the green lure and kill ball with the diameter of 7cm, and treatment 2 is CK (namely the green lure and kill ball with the diameter of 7 cm), and each zone has the total amount of the lure and kill ball with 5 lure and kill ball.
d. Comparison of the Effect of Eucalyptus citriodora essential oil with other types of attractants
The method comprises the steps of using a non-woven cotton piece with the radius of 1.5cm as a carrier to prepare a trapping core, simultaneously using a non-woven cotton piece with the radius of 1.5cm as a carrier to prepare a trapping core, and placing different trapping cores into green trapping and killing balls with the diameter of 7cm in the last ten days of 6 months, wherein CK is the trapping ball with the diameter of 7cm, does not add the trapping core, has odor release holes at the bottom of the trapping and killing ball with the diameter of 7cm, hanging the trapping and killing balls in the field, repeating 10 times for each treatment, and observing the number of adult citrus fruit flies on different trapping and killing balls, so that the trapping and killing effect of the trapping and killing core and the trapping and killing ball made of the eucalyptus citriodora is higher than that of the trapping and killing ball made of the limonene and the trapping and killing ball, and the fruiter and the trapping and killing ball are obviously higher than that of the sugar solution, the yeast hydrolysis protein.
Attached table 3 influence of different luring cores on citrus fruit fly luring effect
Treatment of Female insect/head Male worm/head Total up to
Eucalyptus citriodora essential oil lure 16.7±3.55 24.8±4.09 41.5±7.13
Limonene luring core 10.5±4.10 18.8±5.83 29.3±9.64
Sweet and sour liquid lure 5.7±3.07 6.5±2.91 12.2±5.69
Yeast hydrolyzed protein lure 6.8±3.84 7.3±4.34 14.1±7.78
Fruit ruite lure core 7.2±4.82 8.6±4.50 15.8±7.80
CK 10.2±4.31 13.6±5.75 23.8±9.66
Note: the data in the table are the mean number of bactrocera minax on the trap balls ± standard deviation.

Claims (4)

1. The application of eucalyptus essential oil in luring bactrocera minax;
the eucalyptus essential oil is eucalyptus citriodora essential oil;
the eucalyptus essential oil is obtained by steaming and extracting leaves of the eucalyptus citriodora through a rotary evaporator;
dissolving 1mL of eucalyptus essential oil in 6-20mL of solvent with liquid paraffin oil and/or n-hexane as solvent to obtain the attractant for Bactrocera minax.
2. The use according to claim 1, wherein green balls with a diameter of 5-10cm are used as trapping balls for Bactrocera minax, and the trapping core according to claim 1 is placed in the trapping balls.
3. The use according to claim 1, wherein the yellow-green bicolor ball with the diameter of 5-10cm is used as a trapping ball for bactrocera minax, and the trapping core in claim 1 is placed in the trapping ball.
4. Use according to any one of claims 1 to 3, wherein a non-woven cotton sheet is used as a carrier for the wick according to claim 1.
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