CN108902199A - Eucalyptus oil is luring the application in citrus fruit fly - Google Patents
Eucalyptus oil is luring the application in citrus fruit fly Download PDFInfo
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- CN108902199A CN108902199A CN201810946562.3A CN201810946562A CN108902199A CN 108902199 A CN108902199 A CN 108902199A CN 201810946562 A CN201810946562 A CN 201810946562A CN 108902199 A CN108902199 A CN 108902199A
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- Prior art keywords
- citrus fruit
- fruit fly
- lure
- essential oil
- ball
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
- A01M1/04—Attracting insects by using illumination or colours
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses eucalyptus oils to lure the application in citrus fruit fly, and eucalyptus oil is female to citrus fruit fly, male imago all has preferable attractant effect.Eucalyptus citriodora essential oil can directly be developed at efficient attractant or with other attractant composite usages in citrus fruit fly adult controlling or research and development it is new lure immunomodulator compounds to provide new thinking.
Description
Technical field
The invention belongs to field of pest control, and in particular to eucalyptus oil is luring the application in citrus fruit fly.
Background technique
Citrus fruit fly is a kind of destructiveness pest on citrus, is mainly eaten into young fruit, egg hatching at larva with Adult worms producting eggs
Food citrusfruit is caused harm, and larva pupates in the soil after going out fruit, and prevention and treatment is difficult.The most common control method of citrus fruit fly is into
Worm baiting technique mainly includes lure trapping and trap device trapping.Common attractant is from simplest sugar-vinegar liquid to slightly complexity
Yeast protein hydrolysate, started at present in sugar-vinegar liquid, yeast protein hydrolysate be added orange juice, orange peel oil, these lures are all
Belong to food attractant, citrus fruit fly can be trapped to a certain extent, but because its trapping effect is all poorer than viscose glue type trap device effect,
Gradually adhesive type trap device replaces.
Lure and trap device can be combined with each other, but because food attractant is other than trapping and killing citrus fruit fly, Qi Tafei
Target insect can be also attracted, and be will lead to non-target pests on trap device in conjunction with viscose glue type trap device with food attractant and increased,
Reduce the using effect of trap device.Currently, about the patent in terms of citrus fruit fly lure, including food attractant and letter
Plain class lure is ceased, it is early prevention and treatment citrus fruit fly preferable one that Zhao Zhimo etc. (2006), which has invented the sweet and sour formula of liquid of modified form,
Kind food attractant, Niu Changying etc. (2010) obtain a kind of protein bait, Zhang Hongyu etc. by the way that yeast protein hydrolysate is added
(2013) it has also been found that Hansenula anomala can be used as citrus trypetid class attractant.Sex pheromone is a kind of efficient lure ingredient,
Report about citrus fruit fly gyplure has 4, and Shandong red equal (1998), which is extracted, obtains a kind of crude extracts of sex pheromone, but simultaneously
Not yet explicitly active constituent, summer loyalty English extract sex pheromone 2, and 6- di-t-butyl is but ineffective in Field information to phenyl methylcarbamate.
The ingredient of the sex pheromone of all fine jade discovery female adult pest secretions is undecyl alcohol, and laboratory test shows there is attracting action to male imago,
Zhang Hongyu is collected into the volatile materials of citrus fruit fly female adult pest release, isolated R- (+)-lemon also by headspace absorption
Alkene, it is good to the attractant effect of citrus fruit fly male imago that research is found, but two kinds of ingredients do not promote and apply also, and application prospect is not
Know, market is not obtained yet to the gathering effect of citrus fruit fly and is approved.
Summary of the invention
The purpose of the present invention is to provide eucalyptus oil, particularly the new application of eucalyptus citriodora essential oil, i.e. eucalyptus citriodora essential oil exists
Application on citrus fruit fly imago attractant, male imago female to citrus fruit fly all have preferable attractant effect.
The present invention provides eucalyptus oil and is luring the application in citrus fruit fly.Citrus fruit fly refer to citrus fruit fly at
Worm.
The eucalyptus oil is eucalyptus citriodora essential oil or blue gum essential oil.
Preferably, using atoleine and/or n-hexane as solvent, 1mL eucalyptus oil is dissolved in 6-20mL solvent, is made
For the attractant of citrus fruit fly.
Preferably, using the ball of the green of diameter 5-10cm as the trapping ball of citrus fruit fly, lure is placed in trapping ball
It is interior.
Preferably, using the yellow and green ball of diameter 5-10cm as the trapping ball of citrus fruit fly, lure is placed in trapping
In ball.
The eucalyptus oil is to steam one kind raised plant by Rotary Evaporators from the blade of eucalyptus citriodora or blue gum
Object essential oil.
The use concentration of the eucalyptus citriodora essential oil is 8%-20%.
Preferably, the diameter for trapping and killing ball is 6.5-8.5cm, more preferable 7cm.
As an improvement of the above technical solution, the eucalyptus citriodora essential oil is applied to the trapping of citrus fruit fly adult using dense
Degree is 10%, and used solvent is atoleine or n-hexane, i.e., 1ml is added in every 9ml atoleine or n-hexane solvent
Eucalyptus citriodora essential oil.
Eucalyptus citriodora essential oil is a kind of plants essential oil extracted from eucalyptus, and main component is citronellal, rhodinol, lemon alcohol
Deng.It has been reported and shows eucalyptus citriodora essential oil to termite, drilling worm, red flour beetle, mulberry borer, Psacothea hilaris, Monochamus alternatus
Adult etc. has repellent action, often uses in the market as a kind of mosquitos and flies repellent agent, but there is no about eucalyptus citriodora essential oil in mandarin orange
Application on the big trypetid of tangerine, the more not application on citrus fruit fly imago attractant.
The present invention is ground-breaking to propose the new application of eucalyptus citriodora essential oil, is achieved through the following technical solutions：
A applicant using Y-shaped smell sensing apparatus test citrus fruit fly adult to the behavior reactions of different essential oils, different essential oils
It the use of concentration is all 10%.
B compares citrus fruit fly adult to the selection rate of different essential oils, obtains citrus fruit fly adult to eucalyptus citriodora essential oil
Selection rate highest, citrus fruit fly is female, male imago is 50% to the selection rate of eucalyptus citriodora essential oil.
Lure is made in eucalyptus citriodora essential oil by c, together with the suspension of 7cm green trapping ball, carries out the measurement of field attractant effect, hair
What existing eucalyptus citriodora essential oil can significantly improve 7cm green trapping ball lures worm effect, and comprehensive synergistic effect is 31.78%.
D is auspicious with the lure limonene, sugar-vinegar liquid, yeast protein hydrolysate, fruit that there is now by lure made of eucalyptus citriodora essential oil
Spy is respectively placed in 7cm green trapping ball, carries out field attractant effect measurement, it was demonstrated that lure made of eucalyptus citriodora essential oil with
Trapping ball, which is shared, to be higher than limonene to the trapping effect of citrus fruit fly and shares with ball is trapped and killed, and sugar-vinegar liquid, yeast protein hydrolysate,
Fruit Rett and trapping ball share then to be lower than the trapping effect of citrus fruit fly and trapping ball are applied alone, substantially less than eucalyptus citriodora essential oil system
At lure with trapping ball share.
Eucalyptus oil new application provided by the invention, eucalyptus citriodora essential oil can directly be developed at efficient attractant or and its
His attractant composite usage provides new thinking in the new lure compound of citrus fruit fly adult controlling or research and development.
The present invention can also be used together with trapping ball, reduced the quantity of other non-target pests on trapping ball, improved luring for trapping ball
Kill effect.The present invention will not cause damages to natural enemy, people and animals and crop, have many advantages, such as green, environmental protection.
Specific embodiment
The present invention is described in further details below by way of specific embodiment, but should not be understood as present invention protection model
Enclose the limitation of progress.
Embodiment 1：
Application of the eucalyptus citriodora essential oil on citrus fruit fly lure is found by following research：
A. citrus fruit fly is female, male imago is to the behavior reactions of different essential oils for measurement.
The difference essential oil is eucalyptus citriodora essential oil, peppermint essential oil, flores aurantii essential oil, orange leaf essential oil, wormwood essential oil, gardenia
Essential oil, orange essential oil, grapefruit essential oil, limonene, and diluted by atoleine, i.e., essential oil and saxol press 1：10
Ratio mixing；
The method of the test is：1ml essential oil dilution, the quantity of fluid stone such as another arm addition is added in an arm of Y-piece
Wax oil, every kind of essential oil test 20-30 adults, launch 1 test worm from supervisor's mouth every time, when recording each area of test worm stop in 5min
Between and enter each arm number, do not enter into two points of arms and be denoted as in 5min and do not react, it is every to have surveyed Y-piece exchange side after 5 test worms
To every surveyed after a kind of essential oil wipes Y-piece inner wall with dehydrated alcohol.
B. calculate that citrus fruit fly is female, male imago is to the selection rates of different essential oils
The number for entering each arm according to test worm is calculated separately to obtain citrus by the sum of test worm and the test worm quantity of each arm
The selection rate of big trypetid female adult and male worm to every kind of essential oil, the shadow that every kind of essential oil of analysis selects citrus fruit fly female adult and male worm
It rings, concrete outcome sees attached list 1, by subordinate list 1 it is found that citrus fruit fly female adult and male worm are to the selection rate of eucalyptus citriodora essential oil
50%, much higher than the selection rate to other essential oils, also above limonene (the lure ingredients of the inventions such as Zhang Hongyu).
Approach response rate of the 1 citrus fruit fly adult of subordinate list to different essential oils
C. the attractant effect of field verifying eucalyptus citriodora essential oil
After eucalyptus citriodora essential oil is diluted to 10% concentration with saxol, the nonwoven cotton piece for being 1.5cm with radius is carried
Body, is fabricated to lure, and the eucalyptus citriodora essential oil 5ml after dilution is added in every non-woven cotton.In early June, lure is placed in diameter 7cm
In green trapping ball, wherein CK is that lure is not added in diameter 7cm trapping ball, and diameter 7cm traps and kills the odorous relief hole in ball bottom, by it
Hang on field, each processing sets 4 district's groups, and each district's groups set 5 repetitions, and observation has mandarin orange on lure and trapping ball without lure
The quantity of the big trypetid adult of tangerine, discovery have citrus fruit fly adult quantity on the trapping ball of lure to be higher than on the trapping ball without lure
Citrus fruit fly adult quantity can make the trapping effect for trapping and killing ball improve 31% or so, result verification using eucalyptus citriodora essential oil
Eucalyptus citriodora essential oil to citrus fruit fly adult has significant trapping effect (concrete outcome sees attached list 2).
2 eucalyptus citriodora essential oil lure of subordinate list lures diameter 7cm green trapping ball the influence of worm effect
Note：Data are to trap and kill the quantity of citrus fruit fly on ball in table.Wherein, processing 1 lures for lure+diameter 7cm green
It kills, processing 2 is CK (i.e. diameter 7cm green trap and kill ball), and each district's groups are that 5 trapping balls lure worm total amount.
D. eucalyptus citriodora essential oil is compared with the effect of other types attractant
Lure is made as carrier in the nonwoven cotton piece that eucalyptus citriodora essential oil is 1.5cm with radius, while by limonene, sweet and sour
Lure is made as carrier in the nonwoven cotton piece that liquid, yeast protein hydrolysate, fruit Rett are also respectively 1.5cm with radius, in early June,
Different lures are placed in diameter 7cm green trapping ball respectively, wherein CK is that lure is not added in diameter 7cm trapping ball, and diameter 7cm is lured
It kills the odorous relief hole in bottom, is hung on field, each processing sets 10 repetitions, and it is big to observe citrus on different trapping balls
The quantity of trypetid adult, it was demonstrated that lure made of eucalyptus citriodora essential oil and trapping ball share high to the trapping effect of citrus fruit fly
It is shared in limonene and trapping ball, is significantly higher than sugar-vinegar liquid, yeast protein hydrolysate, fruit Rett and trapping ball and shares (concrete outcome
It sees attached list 3).
The different lures of subordinate list 3 lure citrus fruit fly the influence of worm effect
| Processing | Female adult/head | Male worm/head | It is total |
| Eucalyptus citriodora essential oil lure | 16.7±3.55 | 24.8±4.09 | 41.5±7.13 |
| Limonene lure | 10.5±4.10 | 18.8±5.83 | 29.3±9.64 |
| Sugar-vinegar liquid lure | 5.7±3.07 | 6.5±2.91 | 12.2±5.69 |
| Yeast protein hydrolysate lure | 6.8±3.84 | 7.3±4.34 | 14.1±7.78 |
| Fruit Rett lure | 7.2±4.82 | 8.6±4.50 | 15.8±7.80 |
| CK | 10.2±4.31 | 13.6±5.75 | 23.8±9.66 |
Note：Data are to trap and kill the par ± standard deviation of citrus fruit fly on ball in table.
Claims (6)
1. eucalyptus oil is luring the application in citrus fruit fly.
2. application as described in claim 1, which is characterized in that the eucalyptus oil is eucalyptus citriodora essential oil.
3. application as described in claim 1, which is characterized in that using saxol and/or n-hexane as solvent, by 1mL
Eucalyptus oil is dissolved in 6-20mL solvent, the lure as citrus fruit fly.
4. application as claimed in claim 3, which is characterized in that using the ball of the green of diameter 5-10cm as citrus fruit fly
Ball is trapped and killed, lure is placed in trapping ball.
5. application as claimed in claim 3, which is characterized in that using the yellow and green ball of diameter 5-10cm as citrus fruit fly
Trapping ball, by lure be placed in trapping ball in.
6. the application as described in any one of claim 3-5, which is characterized in that make the carrier of lure using nonwoven cotton piece.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810946562.3A CN108902199B (en) | 2018-08-20 | 2018-08-20 | Application of eucalyptus essential oil in attracting bactrocera minax |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810946562.3A CN108902199B (en) | 2018-08-20 | 2018-08-20 | Application of eucalyptus essential oil in attracting bactrocera minax |
Publications (2)
| Publication Number | Publication Date |
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| CN108902199A true CN108902199A (en) | 2018-11-30 |
| CN108902199B CN108902199B (en) | 2020-12-04 |
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| CN201810946562.3A Active CN108902199B (en) | 2018-08-20 | 2018-08-20 | Application of eucalyptus essential oil in attracting bactrocera minax |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63264510A (en) * | 1987-04-20 | 1988-11-01 | Nittec Co Ltd | Termite exterminating agent |
| WO2008101131A1 (en) * | 2007-02-14 | 2008-08-21 | Nonits Llc | Essential oil compositions for killing or repelling ectoparasites and pests and methods for use thereof |
| CN101692836A (en) * | 2009-10-15 | 2010-04-14 | 杜进平 | Slow release attractant and usage of Chinese citrus fly |
| CN102860303A (en) * | 2012-10-15 | 2013-01-09 | 广西工学院 | Method for utilizing citriodora oil to extract waste liquid to prepare mosquito repellent |
| CN105494411A (en) * | 2015-12-18 | 2016-04-20 | 莫博程 | Citrus fruit fly adult trapping and killing agent and using method thereof |
| CN107494536A (en) * | 2017-08-15 | 2017-12-22 | 广东省生物资源应用研究所 | It is a kind of can the effectively medicament of repellent citrus fruit fly female adult pest and its application |
| CN107691497A (en) * | 2017-09-26 | 2018-02-16 | 田阳县群姐砂糖橘专业合作社 | A kind of medicament for preventing and treating citrus fruit fly and preparation method thereof |
| CN107821392A (en) * | 2017-10-11 | 2018-03-23 | 华中农业大学 | A kind of long-acting tablets for having high epigamic to citrus trypetid and preparation method thereof |
-
2018
- 2018-08-20 CN CN201810946562.3A patent/CN108902199B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63264510A (en) * | 1987-04-20 | 1988-11-01 | Nittec Co Ltd | Termite exterminating agent |
| WO2008101131A1 (en) * | 2007-02-14 | 2008-08-21 | Nonits Llc | Essential oil compositions for killing or repelling ectoparasites and pests and methods for use thereof |
| CN101692836A (en) * | 2009-10-15 | 2010-04-14 | 杜进平 | Slow release attractant and usage of Chinese citrus fly |
| CN102860303A (en) * | 2012-10-15 | 2013-01-09 | 广西工学院 | Method for utilizing citriodora oil to extract waste liquid to prepare mosquito repellent |
| CN105494411A (en) * | 2015-12-18 | 2016-04-20 | 莫博程 | Citrus fruit fly adult trapping and killing agent and using method thereof |
| CN107494536A (en) * | 2017-08-15 | 2017-12-22 | 广东省生物资源应用研究所 | It is a kind of can the effectively medicament of repellent citrus fruit fly female adult pest and its application |
| CN107691497A (en) * | 2017-09-26 | 2018-02-16 | 田阳县群姐砂糖橘专业合作社 | A kind of medicament for preventing and treating citrus fruit fly and preparation method thereof |
| CN107821392A (en) * | 2017-10-11 | 2018-03-23 | 华中农业大学 | A kind of long-acting tablets for having high epigamic to citrus trypetid and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| MIYEON JANG 等: "Biological activity of Myrtaceae plant essential oils and their major components against Drosophila suzukii (Diptera: Drosophilidae)", 《PEST MANAGEMENT SCIENCE》 * |
| 屈恋 等: "柠檬桉果实、叶挥发油的成分分析及对比", 《食品工业科学》 * |
| 徐冠仁 主编: "《核农学导论》", 31 March 1997, 原子能出版社 * |
| 李清西 等主编: "《植物保护》", 31 October 2003, 中国农业出版社 * |
| 袁伊旻: "橘小实蝇植物源杀虫和引诱活性物质研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 龚碧涯 等: "诱杀球对橘大实蝇诱捕效果的研究", 《植物保护》 * |
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