CN104379750A - 用于增加重组蛋白分泌的方法 - Google Patents

用于增加重组蛋白分泌的方法 Download PDF

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Publication number
CN104379750A
CN104379750A CN201380020538.2A CN201380020538A CN104379750A CN 104379750 A CN104379750 A CN 104379750A CN 201380020538 A CN201380020538 A CN 201380020538A CN 104379750 A CN104379750 A CN 104379750A
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CN
China
Prior art keywords
concentration
cell
ion
substratum
secretion
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Pending
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CN201380020538.2A
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English (en)
Chinese (zh)
Inventor
W.乔斯特
M.克纳彭伯格
D.克劳斯尼特泽
A.沙亚夫
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Eleva GmbH
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Greenovation Biotech GmbH
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Publication of CN104379750A publication Critical patent/CN104379750A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201380020538.2A 2012-04-17 2013-04-17 用于增加重组蛋白分泌的方法 Pending CN104379750A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12164458.7A EP2653548A1 (de) 2012-04-17 2012-04-17 Verfahren zur Erhöhung der Sekretion rekombinanter Proteine
EP12164458.7 2012-04-17
PCT/EP2013/057956 WO2013156504A1 (de) 2012-04-17 2013-04-17 Verfahren zur erhöhung der sekretion rekombinanter proteine

Publications (1)

Publication Number Publication Date
CN104379750A true CN104379750A (zh) 2015-02-25

Family

ID=48143287

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380020538.2A Pending CN104379750A (zh) 2012-04-17 2013-04-17 用于增加重组蛋白分泌的方法

Country Status (19)

Country Link
US (1) US9850513B2 (enExample)
EP (2) EP2653548A1 (enExample)
JP (1) JP6215305B2 (enExample)
KR (1) KR102028738B1 (enExample)
CN (1) CN104379750A (enExample)
AU (1) AU2013248312B2 (enExample)
BR (1) BR112014025306B1 (enExample)
CA (1) CA2870587A1 (enExample)
DK (1) DK2839012T3 (enExample)
ES (1) ES2755973T3 (enExample)
HR (1) HRP20191876T1 (enExample)
IL (1) IL235052B (enExample)
IN (1) IN2014DN08664A (enExample)
LT (1) LT2839012T (enExample)
MX (1) MX353243B (enExample)
PL (1) PL2839012T3 (enExample)
PT (1) PT2839012T (enExample)
SI (1) SI2839012T1 (enExample)
WO (1) WO2013156504A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2729732B2 (ja) 1992-10-05 1998-03-18 新日本製鐵株式会社 底つき容器の製造方法
EP3808854A1 (de) 2019-10-17 2021-04-21 eleva GmbH Verfahren zur gewinnung von material von pflanzenzelloberflächen
BR112022012467A2 (pt) * 2020-01-31 2022-09-06 Chugai Pharmaceutical Co Ltd Método para produção de composição contendo polipeptídeo com coloração suprimida

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1280186A (zh) * 2000-08-15 2001-01-17 中国水产科学研究院黄海水产研究所 一种新型低温碱性蛋白酶、制造方法 、应用和产生该蛋白酶的微生物
US20100035327A1 (en) * 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63226281A (ja) * 1986-12-26 1988-09-20 Seitai Kinou Riyou Kagakuhin Shinseizou Gijutsu Kenkyu Kumiai 組織培養方法
JP2000245470A (ja) * 1999-02-26 2000-09-12 Noda Inst For Scient Res 植物細胞間液による外来ポリペプチドの製造方法
DE19947290A1 (de) 1999-10-01 2001-04-19 Greenovation Pflanzenbiotechno Verfahren zur Herstellung proteinöser Substanzen
WO2003083066A2 (en) 2002-03-27 2003-10-09 Immunex Corporation Methods for increasing polypeptide production
US8637316B2 (en) * 2008-07-16 2014-01-28 Sumitomo Chemical Company, Limited Glycinin signal sequence for producing secreted proteins in plants

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1280186A (zh) * 2000-08-15 2001-01-17 中国水产科学研究院黄海水产研究所 一种新型低温碱性蛋白酶、制造方法 、应用和产生该蛋白酶的微生物
US20100035327A1 (en) * 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium

Also Published As

Publication number Publication date
JP6215305B2 (ja) 2017-10-18
US9850513B2 (en) 2017-12-26
KR102028738B1 (ko) 2019-10-04
MX2014012407A (es) 2015-04-09
EP2653548A1 (de) 2013-10-23
PL2839012T3 (pl) 2020-05-18
BR112014025306B1 (pt) 2021-11-30
CA2870587A1 (en) 2013-10-24
ES2755973T3 (es) 2020-04-24
SI2839012T1 (sl) 2019-12-31
BR112014025306A2 (pt) 2017-06-20
US20150079632A1 (en) 2015-03-19
MX353243B (es) 2018-01-05
AU2013248312A1 (en) 2014-10-23
KR20150014454A (ko) 2015-02-06
EP2839012B1 (de) 2019-08-28
WO2013156504A1 (de) 2013-10-24
EP2839012A1 (de) 2015-02-25
PT2839012T (pt) 2019-12-05
IN2014DN08664A (enExample) 2015-05-22
JP2015514408A (ja) 2015-05-21
AU2013248312B2 (en) 2018-08-30
LT2839012T (lt) 2019-11-25
IL235052B (en) 2019-12-31
IL235052A0 (en) 2014-12-31
DK2839012T3 (da) 2019-10-21
HRP20191876T1 (hr) 2019-12-27

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Application publication date: 20150225