CN104357312A - Intelligent Rhodospirillum culture device - Google Patents

Intelligent Rhodospirillum culture device Download PDF

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Publication number
CN104357312A
CN104357312A CN201410633098.4A CN201410633098A CN104357312A CN 104357312 A CN104357312 A CN 104357312A CN 201410633098 A CN201410633098 A CN 201410633098A CN 104357312 A CN104357312 A CN 104357312A
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CN
China
Prior art keywords
rhodospirillum
temperature
module
bacterium
controlled
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Pending
Application number
CN201410633098.4A
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Chinese (zh)
Inventor
王猛
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SUZHOU USTC WEILONG INFORMATION TECHNOLOGY Co Ltd
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SUZHOU USTC WEILONG INFORMATION TECHNOLOGY Co Ltd
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Priority to CN201410633098.4A priority Critical patent/CN104357312A/en
Publication of CN104357312A publication Critical patent/CN104357312A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

Abstract

The invention relates to an intelligent Rhodospirillum culture device which is characterized by comprising a bacterium solution tank 1, a nutrient solution tank 2, an alkali solution tank 3, an acid solution tank 4, a pressure pump 5, a control valve 6, a refrigeration module 7, an LED (light-emitting diode) lamp bank 8, a wireless control module 9, a heating module 10, five incubators 11, a water tank 12, a CO2 gas pump 13, a bacterium solution collecting tank 14 and a Rhodospirillum culture execution system 15, wherein the top of each incubator is provided with a bacterium solution, a nutrient solution, an alkali solution and the acid solution control valve 6 and connected to the pressure pump through a plastic pipe; the bottom of each incubator 11 is provided with the bacterium solution collection control valve which is connected to the bacterium solution collecting tank 14 through a plastic pipe; the heating module 10 is installed below the water tank 12; the top of the water tank is provided with the LED lamp bank 8, wireless control module 9 and refrigeration module 7; and the wireless control module is connected to the Rhodospirillum culture execution system.

Description

A kind of intelligent rhodospirillum culture apparatus
Technical field
The present invention relates to a kind of intelligent Photosynthetic bacteria culturing device, belong to reading intelligent agriculture facility technology field.
Background technology
Rhodospirillum is a kind of nutritious, the bacterium be of high nutritive value, containing abundant amino acid in thalline, folic acid, vitamin B group, also has physiologically active substance coenzyme, be applicable to making fertilizer, the additive of feed, the equipment of current cultivation photosynthetic bacterium, major part adopts natural light or incandescent light to provide light source, incubator Plastic Bottle, cultivate easily, culturing process has a big risk, produce bacterium amount and be subject to environmental factors, cause output unstable, based on the nutritive value of rhodospirillum, Applied Computer Techniques, biological illumination technology, the adjustment of temperature and environment, create the biomimetic environment of an applicable rhodospirillum, it is modern plantation, cultivation needs a technology of research.
Summary of the invention
For existing technical deficiency, the object of this invention is to provide a kind of intelligent rhodospirillum culture apparatus, application intelligent wireless network system, biological illumination, the computer of sun running rails and LED intelligence combine, the illumination of simulating nature circle, with semiconductor refrigerating, the function of carbon fiber heating, adjustment temperature, the far infrared rays 8-14um sent when carbon fiber heats, the thermal energy provided, mode is passed to through water, be penetrated in rhodospirillum body, increase the life energy of rhodospirillum, coordinate intelligent control, create the biomimetic environment of rhodospirillum, stable and quantitative cultivation rhodospirillum.
Being a kind of intelligent rhodospirillum culture apparatus for realizing above-mentioned object the present invention, it is characterized in that: 1 bacterium liquid case, 2 nutritive medium casees, 3 lye tanks, 4 acid solution casees, 5 force (forcing) pumps, 6 by-pass valve controls, 7 refrigeration modules, 8LED lamp group, 9 controlled in wireless modules, 10 heating modules, 11 incubators, 12 water tanks, 13C02 add air pump, 14 bacterium liquid collection boxs, 15 photosynthetic bacterias cultivation executive systems compositions.
Wherein LED is made up of 10 optical wavelength 660nm red colored lamp pearls, 10 optical wavelength 450nm blue lamp pearls, 5 optical wavelength 529nm green light pearls;
The incubator that described incubator wherein has 5 glass materials to make, each one of the by-pass valve control of bacterium liquid, nutritive medium, alkali lye, acid solution is equipped with at the top of each incubator, is connected to the force (forcing) pump of outer case with plastics tubing, and bacterium liquid collection valve is equipped with in below;
Wherein said incubator is body of wall, interior paving aluminium foil covering material by polytene heat-insulation plate, collects by-pass valve control plastics tubing be connected with incubator with bacterium liquid collection box at below dress bacterium liquid;
Wherein heating module 10 is contained in below water tank; Tank top is equipped with LED group, controlled in wireless module, refrigeration module; Controlled in wireless module is connected to system host by wireless network card, and bacterium liquid case 1, nutritive medium case 2, lye tank 3, acid solution case 4 are placed on water tank side and are connected with force (forcing) pump 5 by plastics tubing; Controlled in wireless module cultivates executive system through ZigBee communications protocol and rhodospirillum and is connected.
Rate-determining steps is wherein as follows:
(1), by the temperature of controlled in wireless module, the on-the-spot numerical value of PH, EC, CO2 sensor, pass photosynthetic bacteria cultivation executive system back, after carrying out the comparison of parameters, send control signal, controlled in wireless module is transferred to through ZigBee communications protocol, carry out force (forcing) pump, by-pass valve control, heating, the startup of refrigeration module and closing movement, carry out temperature, PH, the supplementing of nutritive medium;
(2), on-off control time of LED group, cultivated the time-angle of the executive system auto computing sun by photosynthetic bacteria, be converted into the time, be modeled to circadian clock body clock, for the different types of biological nature of photosynthetic bacterium, carry out artificially feed;
(3), temperature controls to be made up of semiconductor refrigeration sheet and carbon fiber heating wire, and heat to the water of water tank, lower the temperature, maintain water temperature in the applicable growth temperature of photosynthetic bacterium, mechanical sound during adjustment temperature, maintains the even by temperature of each incubator.
Advantage of the present invention:
Compared with prior art, the present invention adopts the different physiological response of rhodospirillum to illumination wavelength and the landing of the sun, according to the target of cultivation, carry out Intelligent supplemental lighting, with semiconductor refrigerating, with carbon fiber heating, effectively utilize the energy of far infrared rays, increase the growth of rhodospirillum health, with water be insulation carrier, can the samming of maintain environment, reduce hot loss of energy, cultivate executive system by rhodospirillum to be connected with the various sensor wireless of culture apparatus, the data of instant collection culture environment and control, realize long-range training mode, not only reduce aquaculture cost, save manpower and energy consumption, more can increase yield.
Accompanying drawing explanation
Fig. 1 is a kind of intelligent rhodospirillum culture apparatus structure iron;
embodiment
The structure and fuction of a kind of intelligent rhodospirillum culture apparatus of the present invention is described in detail below in conjunction with accompanying drawing 1
1, bacterium liquid case: the original bacteria liquid depositing rhodospirillum;
2, nutritive medium case: deposit the nutritive medium that rhodospirillum is cultivated;
3, lye tank: deposit alkali lye, the liquid of the furnishing that added water by sodium bicarbonate;
4, acid solution case: deposit vinegar liquid;
5, force (forcing) pump: be made up of 4 DC diaphragm pumps, one is connected to bacterium liquid case, nutritive medium case, lye tank, acid solution case separately with plastics tubing, and other end is connected to the corresponding transfer tube of incubator;
6, by-pass valve control: on each incubator, device 3 liquid control valve doors, are connected to corresponding transfer tube by plastics tubing;
7, freeze module: form by containing semiconductor refrigeration sheet, cooling water circulation pipeline;
8, LED group: LED is formed by 10 optical wavelength 660nm red colored lamp pearls, 10 optical wavelength 450nm blue lamp pearls, 5 optical wavelength 529nm green light pearls, is connected with controlled in wireless module with control winding displacement;
9, controlled in wireless module: be made up of ZigBee communication module group, single-chip, rly.;
10, module is heated: be made up of carbon fiber heating wire;
11, incubator: be made up of glass material;
12, water tank: form casing by polytene heat-insulation plate, inwall aluminium foil covering material is coated;
13, CO2 adds air pump: 12V DC fan motor;
14, bacterium collection: form casing by polytene heat-insulation plate, arranges imported valve and outlet valve, wherein imported valve, is connected with incubator discharge valve with plastics tubing.
Its principle of work is as follows:
(1), by the temperature of controlled in wireless module, the on-the-spot numerical value of PH, EC, CO2 sensor, pass photosynthetic bacteria cultivation executive system back, after carrying out the comparison of parameters, send control signal, controlled in wireless module is transferred to through ZigBee communications protocol, carry out force (forcing) pump, by-pass valve control, heating, the startup of refrigeration module and closing movement, carry out temperature, PH, the supplementing of nutritive medium;
(2), on-off control time of LED group, cultivated the time-angle of the executive system auto computing sun by photosynthetic bacteria, be converted into the time, be modeled to circadian clock body clock, for the different types of biological nature of photosynthetic bacterium, carry out artificially feed;
(3), temperature controls to be made up of semiconductor refrigeration sheet and carbon fiber heating wire, and heat to the water of water tank, lower the temperature, maintain water temperature in the applicable growth temperature of photosynthetic bacterium, mechanical sound during adjustment temperature, maintains the even by temperature of each incubator.
The present invention is by the running track of the sun, to the different demand stage of rhodospirillum growth and the landing time of sun every day, carry out artificially feed, by temperature, PH, EC, CO2 sensor, the environmental parameter of automatic backtracking incubator, rhodospirillum cultivation executive system connects through ZigBee communications protocol line traffic control module, calculated by computer intelligence, save human users's intensity, device of solar generating can be added in addition, be combining with green, energy-conservation, biological illumination, intelligentized technology application, not only can save toxigenic capacity, separately can realize the function of remote breeding.
 
Although the present invention discloses as above with preferred embodiment; so itself and be not used to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; when doing a little amendment and perfect, therefore protection scope of the present invention is when being as the criterion of defining with claims.

Claims (3)

1. an intelligent rhodospirillum culture apparatus, is characterized in that: 1 bacterium liquid case, 2 nutritive medium casees, 3 lye tanks, 4 acid solution casees, 5 force (forcing) pumps, 6 by-pass valve controls, 7 refrigeration modules, 8LED lamp group, 9 controlled in wireless modules, 10 heating modules, 11 incubators, 12 water tanks, 13C02 add air pump, 14 bacterium liquid collection boxs, 15 red spiral shell bacterium cultivation executive system compositions.
2. an intelligent rhodospirillum culture apparatus, is characterized in that incubator according to claim 15, and the by-pass valve control 6 of bacterium liquid, nutritive medium, alkali lye, acid solution is equipped with at each top, is connected to force (forcing) pump with plastics tubing; Fill bacterium liquid collection by-pass valve control plastics tubing below incubator 11 and be connected 14 with bacterium liquid collection box; Heating module 10 is contained in below water tank 12; Tank top is equipped with LED group 8, controlled in wireless module 9, refrigeration module 7; Controlled in wireless module is connected to system host by wireless network card, and bacterium liquid case 1, nutritive medium case 2, alkali lye 3, acid solution are placed on water tank side for 4 casees and are connected with force (forcing) pump 5 by plastics tubing; Controlled in wireless module cultivates executive system through ZigBee communications protocol with red spiral shell bacterium and is connected.
3. realize the one intelligence rhodospirillum culture apparatus master mode of claim 1, its spy is:
(1), by the temperature of controlled in wireless module, the on-the-spot numerical value of PH, EC, CO2 sensor, pass photosynthetic bacteria cultivation executive system back, after carrying out the comparison of parameters, send control signal, controlled in wireless module is transferred to through ZigBee communications protocol, carry out force (forcing) pump, by-pass valve control, heating, the startup of refrigeration module and closing movement, carry out temperature, PH, the supplementing of nutritive medium;
(2), on-off control time of LED group, cultivated the time-angle of the executive system auto computing sun by photosynthetic bacteria, be modeled to circadian clock body clock, for the different types of biological nature of red spiral shell bacterium, carry out artificially feed;
(3), temperature controls to be made up of semiconductor refrigeration sheet and carbon fiber heating wire, and heat to the water of water tank, lower the temperature, maintain water temperature in the applicable growth temperature of red spiral shell bacterium, mechanical sound during adjustment temperature, maintains the even by temperature of each incubator.
CN201410633098.4A 2014-11-12 2014-11-12 Intelligent Rhodospirillum culture device Pending CN104357312A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410633098.4A CN104357312A (en) 2014-11-12 2014-11-12 Intelligent Rhodospirillum culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410633098.4A CN104357312A (en) 2014-11-12 2014-11-12 Intelligent Rhodospirillum culture device

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CN104357312A true CN104357312A (en) 2015-02-18

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101629142A (en) * 2009-08-11 2010-01-20 王科 LED light source bioreactor
CN103607829A (en) * 2013-12-05 2014-02-26 苏州科大微龙信息技术有限公司 Intelligent network type plant growth LED lamp capable of adjusting light quality automatically
CN203904331U (en) * 2014-05-12 2014-10-29 上海海洋大学 Intensive-mode microalgae photobioreactor device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101629142A (en) * 2009-08-11 2010-01-20 王科 LED light source bioreactor
CN103607829A (en) * 2013-12-05 2014-02-26 苏州科大微龙信息技术有限公司 Intelligent network type plant growth LED lamp capable of adjusting light quality automatically
CN203904331U (en) * 2014-05-12 2014-10-29 上海海洋大学 Intensive-mode microalgae photobioreactor device

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
宦娟等: "基于ZigBee 的水产养殖水环境无线监控系统设计", 《渔业现代化》, vol. 39, no. 1, 31 December 2012 (2012-12-31) *
徐明芳: "光生物反应器内设LED 光源的特性研究", 《食品与发酵工业》, vol. 27, no. 4, 31 December 2001 (2001-12-31), pages 7 - 11 *

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Application publication date: 20150218