CN203319995U - Algae culture device - Google Patents

Algae culture device Download PDF

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Publication number
CN203319995U
CN203319995U CN2013202457864U CN201320245786U CN203319995U CN 203319995 U CN203319995 U CN 203319995U CN 2013202457864 U CN2013202457864 U CN 2013202457864U CN 201320245786 U CN201320245786 U CN 201320245786U CN 203319995 U CN203319995 U CN 203319995U
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CN
China
Prior art keywords
house
transparent tube
algatron
possesses
nutrient solution
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CN2013202457864U
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Chinese (zh)
Inventor
长濑俊哉
沼沢徹
大桥英治
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BIOLOGICAL GENE KKOF JAPAN
Nissui Corp
Original Assignee
BIOLOGICAL GENE KKOF JAPAN
Nippon Suisan Kaisha Ltd
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The utility model provides an algae culture device which is not easily influenced by weather. The algae culture device (100) comprises a circulation pipeline (C) provided with a transparent tube (1) arranged by extending to the outside of a house (50) from the inside of the house (50), a pump (5) used for circulating a culture solution in the circulation pipeline (C), and a light source (20) used for irradiating light on a part (1a) arranged in the house (50) within the transparent tube (1).

Description

Algatron
Technical field
The utility model relates to a kind of algatron.
Background technology
At present, known have a device of cultivating the algae such as haematococcus pulvialis.
The prior art document
Patent documentation
Patent documentation 1: Japanese Unexamined Patent Application Publication 2000-523938 communique
Existing device is according to the significantly variations such as its throughput of change of the factors such as weather.
The utility model content
The utility model is to propose in view of the above problems, and its purpose is to provide a kind of algatron that is not subject to weather effect.
According to algatron of the present utility model, comprising: circulating line possesses the transparent tube that extends in house house and arrange; Pump, make nutrient solution at described circulating line internal recycle; And light source, be arranged in the part irradiation light in described house in described transparent tube.
According to the utility model, by through being arranged in the algae irradiation sunlight of part house outside (below, be called transparent tube outside room) in transparent tube, can utilize natural energy resources effectively to cultivate algae.And, when the illuminance outside the rooms such as cloudy day, rainy day, morning and evening is not enough, be arranged in part in house (below, be called transparent tube within doors) in transparent tube, can be by Light Source Compensation the light to algae, so production management is easier to.And light source arrangement in doors, is easily protected light source thus from wind and rain or dust etc.
At this, preferably, algatron also possesses: optical sensor, obtain the brightness outside house; And control part, the brightness based on outside described house, control the light intensity by described light source irradiation.Accordingly, easily move algatron.
And preferably, when the brightness outside described house is more than or equal to predetermined threshold value, described control part is closed described light source, when the brightness outside described house does not reach predetermined threshold value, described control part is lighted described light source.And preferably, when described light source is lit, described control part changes the light intensity by light source irradiation, makes by the light intensity of described light source irradiation stronger when lower with the brightness outside described house.Accordingly, corresponding to the insufficient section to the light quantity of nutrient solution in the house external radiation exposure, can effectively compensate light intensity.
And preferably, algatron also possesses the cooling end be arranged in house, the nutrient solution in cooling described circulating line.
And preferably, algatron also possesses the gas-to-liquid contact section be arranged in described house, make the nutrient solution contact gas in described circulating line.
And preferably, algatron also possesses the extraction opening be arranged in described house, in described circulating line, extracts nutrient solution.
And preferably, algatron also possesses the house of being arranged in Nei De climate regulated room, this climate regulated room possesses the air regulator of adjusting the temperature in climate regulated room.
According to algae culture method of the present utility model, comprise: in possessing the circulating line that extends to the transparent tube of arranging house in house, the operation that nutrient solution is circulated; And the operation of to the part be disposed in described house, irradiating light in described transparent tube.
At this, preferably, aforesaid method also possesses the brightness based on outside house and controls the control operation of described light intensity.
And preferably, the described control operation of aforesaid method is, when the brightness outside described house is more than or equal to predetermined threshold value, do not irradiate described light, when the brightness outside described house does not reach predetermined threshold value, irradiate described light.
In addition, described control operation is, when irradiating the described light time, can change light intensity, when lower with the brightness outside described house, makes described light intensity stronger.
And preferably, aforesaid method also possesses: be arranged in the part in described house in described circulating line, make the operation of described nutrient solution contact gas.
And preferably, aforesaid method also possesses: the operation that is arranged in the part extraction nutrient solution in described house from described circulating line.
According to the utility model, can provide the algatron and the method that are not subject to weather effect.
The accompanying drawing explanation
Fig. 1 is the front view according to the algatron of embodiment of the present utility model.
Fig. 2 is the side-view of the algatron of Fig. 1.
Fig. 3 is the vertical view of the algatron of Fig. 1.
Fig. 4 is that explanation is controlled the chart from an example of the light intensity of light source according to control part.
Fig. 5 is the front view according to the algatron of other embodiments.
Main nomenclature:
50 houses
1 transparent tube
1a is transparent tube within doors
Transparent tube outside the 1b room
The C circulating line
5 pumps
20 light sources
60 optical sensors
65 control parts
76 air regulators
77 water cooling sections
40 vapour-liquid contacting columns (gas-to-liquid contact section)
40b covers (extraction opening)
100 algatrons
Embodiment
Below, with reference to accompanying drawing, embodiment of the present utility model is described.Fig. 1 is the front view according to the algatron 100 of embodiment of the present utility model.
Mainly possess house 50, circulating line C, pump 5, light source 20, optical sensor 60 and control part 65 according to the algatron 100 of present embodiment.
House 50 possesses top cover and wall, makes to be arranged at the impact that inner object is avoided the environment (for example, sunlight, rain, wind, dust etc.) outside room.
Circulating line C is comprised of pipeline 3a, head 2a, a plurality of transparent tube 1, head 2b, a plurality of transparent tube 1, head 2c, a plurality of transparent tube 1, head 2d, a plurality of transparent tube 1, head 2e, pipeline 3b, vapour-liquid contacting column 40 and pipeline 3c.The nutrient solution that head 2a will flow into from pipeline 3a is assigned to a plurality of transparent tube 1. Head 2b, 2c, 2d mix respectively the nutrient solution flowed into from a plurality of transparent tube 1, and then are assigned to a plurality of transparent tube 1.The nutrient solution that head 2e will flow into from a plurality of transparent tube 1 is supplied to pipeline 3b.
Transparent tube 1 possesses transparent tube within doors (being arranged in the part in house 50 transparent tube) 1a, outer transparent tube (being arranged in the part outside house 50 in the transparent tube) 1b in room and transparent tube (being arranged in the interior part in house 50 in transparent tube) 1a within doors successively from upstream side respectively.Transparent tube 1a is not subject to the direct projection of sunlight within doors, and outside contrary room, transparent tube 1b is subject to the direct projection of sunlight.
Preferably, in circulating line C, the summation of the total length of transparent tube 1b outside the total length of relative transparent tube 1a within doors and room, the shared ratio of the total length of transparent tube 1a is 10~25% within doors.
Preferably, in circulating line C, outside the total length of transparent tube 1a and room, the summation of the total length of transparent tube 1b is 10~70m within doors.
And preferably, outside transparent tube 1a and room, the internal diameter of transparent tube 1b is 40mm~80mm within doors.Material to these transparent tube is not particularly limited, and transparent getting final product under visible ray, can exemplify glass, acrylic resin.
As shown in Figures 2 and 3, transparent tube 1a extends along horizontal direction respectively within doors.And, as shown in Figure 3, when outside room, transparent tube 1b sees from above, possessing the U word shape, extend along horizontal direction at the both ends except central circular arc shaped portion.And as shown in Figure 1, a plurality of transparent tube 1 that are connected in a head are arranged in parallel into row along vertical direction.Further, be furnished with head 2c, head 2e on the top of head 2a, the top of head 2b is furnished with head 2d, as shown in Figure 3, in house 50, the row of transparent tube 1a and be formed with gap V between the row of transparent tube 1a within doors within doors.Transparent tube 1 is supported by brace table 10.
As shown in Figure 1,50 inside in house, transparent tube 1a and head 2a~2e are disposed in and are arranged at house 50Nei De climate regulated room 70 inside within doors.Climate regulated room 70 possesses air regulator 76, and the temperature in climate regulated room 70 is adjusted into to suitable temperature, for example 0~20 ℃.
The groove that vapour-liquid contacting column (gas-to-liquid contact section) 40 is the storage part nutrient solution, possess gas distributor 12 in inside.Gas distributor 12 is connected to air pump 71 and carbonic acid gas supply source 72 by pipeline 75.The amount of air can be regulated by the valve 73 that is connected in pipeline 75, and the amount of carbonic acid gas can be regulated by the valve 74 that is connected in pipeline 75.As carbonic acid gas supply source 72, can exemplify liquid CO 2High-pressure gas cylinder.In vapour-liquid contacting column 40, by the gas-to-liquid contact via gas distributor 12, carbonic acid gas can be supplied in nutrient solution.And the top of vapour-liquid contacting column 40 is provided with removable lid 40b, thereby can change nutrient solution.
Vapour-liquid contacting column 40 is arranged in and further is arranged in 50Nei De clean room, house 80, when lid 40b is opened, prevents that nutrient solution from being polluted.Clean room 80 possesses the strainers such as high efficiency particulate air filter (HEPA filter).Preferably, clean room 80 possesses clean extraneous air is imported to indoor air purifier 82.Further preferably, clean room 80 possesses the ultraviolet germicidal lamp 84 of killing indoor bacterium.
Pump 5 is arranged between pipeline 3c and pipeline 3a, makes nutrient solution at circulating line C internal recycle.Circulation velocity can be in 0.1~1m/s left and right.
50 inside in house, light source 20 is arranged in above-mentioned gap V, and transparent tube 1a is within doors irradiated to light.Light source 20 is had no particular limits, get final product so long as can irradiate the source of artificial light of the light of the required wavelength of the growth of algae.As such light source 20, can exemplify LED.Wherein be preferably the blue led of wavelength region at about 470nm.And preferably, a plurality of 1a of transparent tube within doors that are arranged in parallel for light is shone efficiently, used LED to be arranged to the light source of matrix shape.Especially preferably, LED is arranged to respectively matrix shape on the two sides of plate-shaped member, can irradiate light from two sides like this.For the shape of light source 20 or the kind of LED etc., be not particularly limited.
Optical sensor 60 is arranged without, obtains sun light intensity (that is, the brightness outside house), such as the illuminance outside room, radiant illumination etc.Brightness outside the room that control part 65 obtains based on optical sensor 60, control the light that light source 20 is exported.
Thus, under the situation of the undercapacity as sunlights such as cloudy day and rainy days (comparing fine day), can compensate the amount of the photon that gives nutrient solution.And, even at fine day, in periods such as morning of the undercapacity of comparing noon and front and back sunlight thereof, dusks, can compensate photon equally.Especially, even the sun light intensity of the noon while waiting fine day in the winter time and front and back thereof also under not enough situation, can compensate to the amount of photon the condition identical with summer etc.
Particularly, such as the brightness outside room, during lower than predetermined threshold value (illuminance, photon flux density etc.), control part (computer) 65 can switch to driving condition from non-driven state by light source 20, switches to illuminating state.And, when the brightness outside room is more than or equal to predetermined threshold value (illuminance, photon flux density etc.), control part 65 can switch to non-driven state from driving condition by light source 20, switches to closing condition.
And when light source 20, when driving (lighting) state, control part 65 can change the light intensity irradiated by light source 20, makes by the light intensity of light source 20 irradiations stronger when lower with brightness without.Control part 65 can arrange a plurality of threshold values, and periodically driving light source 20, what with the light that makes to be irradiated by light source 20 when threshold value is lower, become is stronger, for example, sun light intensity outside room is during lower than predefined first threshold (illuminance, photon flux density etc.), the light of driving light source 20 output predetermined strengths, when sun light intensity during lower than predefined Second Threshold (being less than first threshold), the more high-intensity light of driving light source 20 output.And in the same manner, light intensity can also change along with the function of the brightness of take outside the room linear function that is variable or quadratic function etc.
Further, for example, shown in Fig. 4, the amount of the change that the trend corresponding to morning, at dusk the time causes or the sunlight intensity not enough (13 left and right in figure) that causes because of cloud layer etc. is carried out driving light source 20, so that give the amount of the photon of nutrient solution, immobilizes.
At this, it should be noted that, control part 65 can be at driving light source 20 not at night, to be set dark period.
Then, the algae culture method of using according to the algatron of above-mentioned embodiment is described.
At first, supply with nutrient solution in circulating line C.Nutrient solution comprises as the necessary medium component of the propagation of the algae of seed and algae.
For algae, have no particular limits, so long as the algae grown by photosynthesis gets final product, haematococcus pulvialis particularly preferably.Haematococcus pulvialis preferably belongs to the frond of the production astaxanthin of haematococcus.Particularly, Haematococcus pluvialis (Haematocoocus Pluvialls), Haematococcus lacustris (haematococcus pulvialis is given birth in lake), Haematococcus capensis (haematococcus pulvialis is given birth in sea), Haematococcus droebakensis, Haematococcus zimbabwiensis etc. are arranged.More specifically, can enumerate Haematocoocus Pluvialls NIES144, the living haematococcus pulvialis ATTC30402 in lake, ATCC30453, IAMC296, IAM392, IAM393, IAM394, IAM399, the living haematococcus pulvialis UTEX1023 in sea, Haematococcus droebakensis UTEX55, Haematococcus zimbabwiensis UTEX1758 etc.
Medium component can make algae proliferation get final product, and suitably sets and get final product corresponding to kind of algae etc.
Supply with this nutrient solution from the lid 40b (extraction opening) of the vapour-liquid contacting column 40 that is positioned at clean room 80 to circulating line C.Vapour-liquid contacting column 40 is arranged in house 50, can prevent thus the pollution of nutrient solution.When particularly vapour-liquid contacting column 40 is positioned at clean room 80, can further prevent from polluting.
Then, driving pump 5 makes nutrient solution at circulating line C internal recycle.Solar irradiation is mapped to the nutrient solution through transparent tube 1b outside room, by photosynthesis, breeds algae.And, when because of reasons such as cloudy day, rainy days, causing the luminance shortage outside room, detect these situations by optical sensor 60, and then control part 65 driving light sources 20 are with the light of undercompensation.
Accordingly, when utilizing sunlight, be not subject to the impact of seasonal variations such as the fluctuation of climate of short-term or rainy season/dry season etc., can stably breed algae.
And light source 20 is arranged within doors, can from the outside atmosphere of sunlight, rain, wind etc., be easy to protect light source 20 thus, and then can extends the work-ing life of light source 20.
The desirable culture temperature of nutrient solution according to the green alga of using and difference is generally 5~50 ℃, is preferably 10~37 ℃, more preferably 15~30 ℃.By the control of the temperature in climate regulated room 70, can suitably reduce and flow through the temperature of the nutrient solution of transparent tube 1a within doors, thereby the temperature of nutrient solution is also manageable.
After the propagation operation of the haematococcus pulvialis through in nutrient solution, carry out the generation of astaxanthin and accumulate operation, obtain cystocyte (cyst cell).This operation applies with formation at the pressure that controls environment under the condition of environment of pressure of the growth that suppresses cell carries out.That is, apply separately or in combination the interpolation of nutrition source exhaustion, salt, the environmental stresss such as irradiation of light, thereby can make vegetative cell be changed to rapidly the cystocyte form in dormant state.And the chemical activators system is excited by the exhaustion of nutrition source, the irradiation of light, accumulates a large amount of astaxanthins in cystocyte.Cystocyte can easily reclaim from the valve of the bottom of the lid 40b that is arranged at vapour-liquid contacting column 40 or vapour-liquid contacting column 40 etc.
The utility model is not limited to above-mentioned embodiment, can carry out various distortion.For example, in the above-described embodiment, circulating line C possesses head and is bifurcated into a plurality of transparent tube 1 midway, but also bifurcated not midway.For example, and in the situation that circulating line C is bifurcated, a plurality of transparent tube 1 of bifurcated can not adopt the mode of vertical arrangement, can be horizontal modes.Form for circulating line C is not particularly limited.
And, in the above-described embodiment, control the temperature of nutrient solution by the temperature of being adjusted in climate regulated room 70 by air regulator 76, but also can adopt other modes.For example, replace air regulator 76 or, on the basis that air regulator 76 is set, can adopt the water cooling section (ring sparger etc.) 77 that transparent tube 1b outside room is sprayed to water coolant.And, as shown in Figure 5, also can replace air regulator and water cooling unit 99 is set.Water cooling unit 99 possesses the water jacket 90 on every side that is arranged in vapour-liquid contacting column 40, water jacket 91, watercooler 92, circulating line 99c and the pump 93 on every side that is arranged in head 2a~2e.Circulating line 99c forms the passage that circulates between watercooler 92 and water jacket 90,91.Pump 93 makes water coolant at circulating line 99c internal recycle.Water cooling unit 99 also can only possess any in water jacket 90 or water jacket 91.And, on the formation of Fig. 5, also can add ring sparger 77 etc.And, also can be without recirculated cooling water, such as at water coolant, through after water jacket, being discharged into drainage channel etc.Further, also the releasing type watercooler can be arranged in water jacket 90,91.At this, it should be noted that, sometimes cooling without carrying out according to outside air temperature.
And the shape of light source 20 or configuration are not limited to above-mentioned ad hoc fashion, for example light source 20 also can be arranged to around each transparent tube 1a within doors.
And, for the form of the vapour-liquid contacting column 40 of circulating line C, also have no particular limits, can change gas-liquid and get final product.
And the form of extraction opening also is not limited only to above-mentioned lid 40b, also can within 40 minutes, be arranged with vapour-liquid contacting column.For example, can be provided in the bottom of vapour-liquid contacting column 40 the valve 40V (with reference to Fig. 1) of results, and extract nutrient solution by this valve 40V.
And, although possess optical sensor 60 and control part 65 in above-mentioned embodiment, also can judge the brightness outside house by the people, or control light intensity by the people.

Claims (6)

1. an algatron, is characterized in that, comprising:
Circulating line, possess the transparent tube that extends in house house and arrange;
Pump, make nutrient solution at described circulating line internal recycle; And
Light source is arranged in the part irradiation light in described house in described transparent tube.
2. algatron according to claim 1, is characterized in that, also possesses:
Optical sensor, obtain the brightness outside house; And
Control part, the brightness based on outside described house, control the light intensity by described light source irradiation.
3. algatron according to claim 1 and 2, is characterized in that, also possesses gas-to-liquid contact section, is arranged in house, makes the nutrient solution contact gas in described circulating line.
4. algatron according to claim 1 and 2, is characterized in that, also possesses extraction opening, is arranged in house, in described circulating line, extracts nutrient solution.
5. algatron according to claim 1 and 2, is characterized in that, also possesses cooling end, is arranged in house, the nutrient solution in cooling described circulating line.
6. algatron according to claim 1 and 2, is characterized in that, also possesses climate regulated room, is arranged in house, possesses the air regulator of adjusting the temperature in climate regulated room.
CN2013202457864U 2012-08-22 2013-05-08 Algae culture device Expired - Lifetime CN203319995U (en)

Applications Claiming Priority (2)

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JP2012183050A JP5919579B2 (en) 2012-08-22 2012-08-22 Algal culture apparatus and culture method
JP2012-183050 2012-08-22

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Cited By (1)

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CN107841448A (en) * 2017-12-14 2018-03-27 山西农业大学 The energy-conservation bioreactor of outside scenery microalgae

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KR101593178B1 (en) 2014-06-30 2016-02-16 한국생산기술연구원 The system of mass culture microalgae
JP6164325B2 (en) * 2016-03-25 2017-07-19 コニカミノルタ株式会社 Printing system, printing method, and printing program
KR102273724B1 (en) * 2019-07-10 2021-07-06 강원도 삼척시(농업기술센터장) Continuos incubating apparatus for microalgae capable of selective controlling of cultivation environment

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JP2011200177A (en) * 2010-03-26 2011-10-13 Mitsui Eng & Shipbuild Co Ltd Culturing device and culturing method
JP2011254725A (en) * 2010-06-07 2011-12-22 Sumitomo Heavy Ind Ltd Photosynthetic microalgae culture apparatus
JP2012044969A (en) * 2010-08-30 2012-03-08 Paleo Labo Co Ltd Method for producing euglena containing no carbon isotope 14c, euglena containing no carbon isotope 14c, method for raising laboratory animal containing no carbon isotope 14c, laboratory animal containing no carbon isotope 14c

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Publication number Priority date Publication date Assignee Title
CN107841448A (en) * 2017-12-14 2018-03-27 山西农业大学 The energy-conservation bioreactor of outside scenery microalgae

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