CN104066832B - Positive-energy photobioreactor device and method using such a photobioreactor - Google Patents

Positive-energy photobioreactor device and method using such a photobioreactor Download PDF

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Publication number
CN104066832B
CN104066832B CN201380004180.4A CN201380004180A CN104066832B CN 104066832 B CN104066832 B CN 104066832B CN 201380004180 A CN201380004180 A CN 201380004180A CN 104066832 B CN104066832 B CN 104066832B
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CN
China
Prior art keywords
culture medium
microalgae
honeycomb plate
effluent
transparent honeycomb
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Expired - Fee Related
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CN201380004180.4A
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Chinese (zh)
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CN104066832A (en
Inventor
让-路易斯·金德勒
皮埃尔·托齐纳
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En Neng System House
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En Neng System House
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • EFIXED CONSTRUCTIONS
    • E04BUILDING
    • E04BGENERAL BUILDING CONSTRUCTIONS; WALLS, e.g. PARTITIONS; ROOFS; FLOORS; CEILINGS; INSULATION OR OTHER PROTECTION OF BUILDINGS
    • E04B1/00Constructions in general; Structures which are not restricted either to walls, e.g. partitions, or floors or ceilings or roofs
    • E04B1/62Insulation or other protection; Elements or use of specified material therefor
    • E04B1/74Heat, sound or noise insulation, absorption, or reflection; Other building methods affording favourable thermal or acoustical conditions, e.g. accumulating of heat within walls
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24HEATING; RANGES; VENTILATING
    • F24SSOLAR HEAT COLLECTORS; SOLAR HEAT SYSTEMS
    • F24S20/00Solar heat collectors specially adapted for particular uses or environments
    • F24S20/60Solar heat collectors integrated in fixed constructions, e.g. in buildings
    • F24S20/66Solar heat collectors integrated in fixed constructions, e.g. in buildings in the form of facade constructions, e.g. wall constructions
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F24HEATING; RANGES; VENTILATING
    • F24SSOLAR HEAT COLLECTORS; SOLAR HEAT SYSTEMS
    • F24S90/00Solar heat systems not otherwise provided for
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02BCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
    • Y02B10/00Integration of renewable energy sources in buildings
    • Y02B10/20Solar thermal
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E10/00Energy generation through renewable energy sources
    • Y02E10/40Solar thermal energy, e.g. solar towers

Abstract

The invention relates to a method for treating an effluent (311, 313), characterized in that it comprises the steps consisting in: a. preparing an aqueous culture medium comprising microalgae, carbon dioxide (311) and mineral nutrients; b. injecting said culture medium into a closed circuit (135) exposed to light; c. measuring the concentration of microalgae in said culture medium in circulation; d. when the concentration of microalgae reaches a threshold value, harvesting an amount of the culture medium in circulation; e. separating (450) the water (414) and the microalgae (415) from said amount; f. using the microalgae recovered as a source of energy. The invention also relates to a device for carrying out this method.

Description

Positive energy bioreactor device and the method using the bioreactor
The present invention relates to a kind of positive energy bioreactor device and the method using the bioreactor.More specifically Ground, but not exclusively, the present invention is adapted for carrying out being particularly useful for the active thermal insulation board of external surface of buildings.
File WO2010072925 describes a kind of including especially on external surface of buildings and including microdisk electrode Transparent pipe device.The prior art device makes it possible to process the liquid or gaseous effluent for including carbon dioxide.Term " process " is that the effect of the biomass by being made up of microalgae converts the effluent, is existed more particularly by photosynthesis Carbon dioxide is fixed in the biomass.The prior art device is effective for the process of effluent.However, described device It is power consumption, and complexity is installed.
Present invention seek to address that the defect of prior art, and therefore relate to a kind of method for processing effluent, institute The method of stating is comprised the following steps:
A. the aqueous culture medium for including microalgae, carbon dioxide and mineral nutrition thing is prepared;
B. culture medium injection is exposed in the closed-loop path of light;
C. the concentration of the microalgae circulated in the culture medium is measured;
D. when the concentration of microalgae reaches threshold value, a certain amount of Cyclic culture medium is harvested;
E. water and microalgae are separated from the Cyclic culture medium of the amount;
F. the microalgae for reclaiming is used as energy source.
Therefore, goal approach of the invention is to produce microalgae from luminous energy and waste.The microalgae is used as fuel to produce Life is at least corresponded to for a) the step of the goal approach of the application present invention to big energy f).Therefore, target of the invention Method is referred to as positive energy.
The present invention is advantageously carried out according to following independent considerations or according to the combination of all effective technologies embodiments.
Advantageously, step a) comprises the steps:
Ai. effluent micronized is made in aqueous.
Therefore, nutrients is reduced to tiny bubble, and the exchange surface between biomass and nutrients increases, so that Obtain nutrients and be consumed and cause micro-algae propagation in the best way.
According to one embodiment, the effluent is lixivium.
According to advantageous embodiment, the effluent is methanation biogas residue and biogas liquid, therefore, this is somewhat made according to prior art Waste rises in value, enabling produce energy using the goal approach of the present invention.
According to another advantageous embodiment, the effluent is made up of the sewage of building.
According to sought effect, above-described embodiment is advantageously combined so that culture medium is optimized.According to these enforcements The advantage of effluent that example is used is especially to have been digested in advance by bacterium, to remove quite a few Organic Content, and because This is rich in can be with photosynthesis and the mineral nutrition thing deposited.
D) comprises the steps according to the step of advantageous embodiment, the goal approach of the present invention:
Di. flocculation is included in the microalgae in culture medium.
Therefore, it is easier to obtain microalgae, and the consumption of energy less than membrane filtration technique.
The di according to the step of particularly advantageous embodiment, the goal approach of the present invention) undergone by making the culture medium Electric pulse is realizing.The embodiment is especially economical, because only consuming about 15% in the energy by the microalgae release for obtaining.This Outward, it is to avoid introduce product in culture medium.Methods described also allows for extracting the lipid of the microalgae.
The advantageous embodiment of goal approach of the invention, by making pipeline of the culture medium outside the building on surface It is middle to circulate to realize step b).Therefore, culture medium is exposed in large area light with minimum shade, and is not take up ground location.
The embodiment of goal approach of the invention, step b) comprises the steps:
Bi. control is the luminous intensity that the microalgae is undergone during culture medium is circulated in the pipeline.
Therefore, the existence and breeding of the microalgae colony of the culture medium is controlled.
According to the favourable realization of the embodiment, step bi is realized by the colored fluid circulation for making to be limited in train of thought), Wherein described train of thought is included in light and exposes to the open air between source and culture medium circulating line wall.Therefore, realized in economical and beautiful mode The regulation of the light energy to being absorbed.Colored fluid also acts as heat transport fluid, to reclaim the heat of culture medium and by heat Amount is for example sent to the device for manufacturing health hot water.
The particularly advantageous embodiment of goal approach of the invention, the effluent includes organic nutrient substance, and Microalgae is mixotrophic, and the goal approach of the present invention comprises the steps:
Bii. reduce culture medium to be exposed to light to be conducive to the heterotrophism behavior of microalgae and consume organic matter;
Biii. the light exposure of culture medium is increased, to be conducive to the autotrophy behavior of microalgae and be conducive to the effluent Mineral matter digestion and photosynthesis.
On the one hand the embodiment makes it possible to process the waste not digested in advance by bacterium, such as by methanation. Additionally, having benefited from lacking light exposure, heterotrophism behavior makes it possible to produce more substantial biomass.Therefore, by adjusting light exposure, The goal approach of the present invention makes it possible to obtain the energy benefits of all the elements thing of effluent, especially when the content is that have When machine mixing and inorganic mixing, such as in the case of sanitary sewage.
Advantageously, the effluent comprising organic nutrient substance is realized from food waste.
The invention further relates to a kind of for using the device of the goal approach of the present invention, the device to include:
I. the first transparent honeycomb plate that can be fixed on external surface of buildings, and for making to include the culture of microalgae The device of medium circulation, the circulating line of the culture medium includes the honeycomb of the plate.
Therefore, the honeycomb of the plate is used for culture medium and follows along the large surface culture medium on external surface of buildings Ring.Embodiment plate whether in pipeline manufacture or on the outer surface is all especially economical in installing.
Advantageously, destination apparatus of the invention include:
Ii. the second transparent honeycomb plate being exposed on the face of light for being fixed on first cellular board adjusts transparent honeybee Nest plate, and the device for making colored fluid circulate in the honeycomb of second cellular board.
Therefore, circulation of the colored fluid in the second plate being connected with the first plate, plays a part of screen and makes Must can control microalgae and be exposed to the amount of light therein, and the Xanthophyll cycle for enabling in particular to control to be observed in some microalgaes shows As.
Advantageously, destination apparatus of the invention include:
Iii. be fixed on the face relative with the stationary plane of second cellular board on first cellular board Three transparent honeycomb plates adjust transparent honeycomb plate, and for making what colored fluid was circulated in the honeycomb of the 3rd cellular board Device.
Therefore, by the light absorbs on the inner face for controlling the plate, the destination apparatus of the present invention are enabled control over by too Sun radiation brings the heat of culture medium.
Advantageously, destination apparatus of the invention include:
Iv. the heat exchanger between the culture medium for circulating in the honeycomb of the first plate and heat transport fluid.
Therefore, the temperature of the culture medium is conditioned, and the heat of the culture medium is recycled.
Referring to figs. 1 to 4, the present invention is described below according to not in any way limiting preferred embodiment, wherein:
- Fig. 1 shows that the realization of the destination apparatus of the invention being applied on external surface of buildings is shown with front schematic view Example;
- Fig. 2 illustrates the implementation example of the cellular board of device of the invention with the AA section views limited in Fig. 1;
- Fig. 3 schematically shows the culture medium in the closed circuit of the destination apparatus for preparing the present invention to be implanted Example;With
- Fig. 4 schematically shows the extraction of the microalgae of the device of implementation example of the invention, separates and value-added Operation.
Fig. 1, according to implementation example, the destination apparatus of the present invention include multiple outer surfaces for being fixed on building (100) (120) cellular board (110) on.Each plate (110) is included by sealing multiple honeycombs (111) that inwall is isolated from each other, honeycomb (111) conduit of fluid is constituted.The honeycomb (111) of different plates (110) is hydraulic communication, so as to realize fluid in building The circulation (135) in the honeycomb on the whole outer surface (120) of thing (100).According to the implementation example, it is being fixed on wall All plates between realize the circulation (135).Alternatively, multiple streams are organized with the subset of cellular board.
According to the implementation example, the circulation (135) of fluid, and the honeycomb of the plate (110) are substantially perpendicularly realized Thus be directed.According to optional embodiment, the circulation is essentially horizontally realized.Follow in the honeycomb (111) of the plate The fluid of ring is comprising microalgae, nutrients and carbon dioxide (CO2) aqueous culture medium.Made by means of pumping (150) described Fluid circulation, and according to implementation example, the fluid undergoes sun spoke during advancing on building (100) outer surface (120) Penetrate.It is exposed to solar radiation and have activated absorption and photosynthesis phenomenon of the algae to nutrients, photosynthesis makes it possible to fix institute The carbon in algae is stated, algae passes through cell division from synthetic organic molecule, such as aliphatic acid in the carbon and mineral nutrition thing, the algae Breeding.These lipids are energy sources.
The effect of solar radiation is additionally operable to the temperature of the culture medium for increasing circulation, and the temperature is likely to be breached and microalgae The incompatible rank of existence.Equally, the temperature of the culture medium is adjusted.
According to first embodiment, by advantageously cooling down circulation of fluid by means of heat exchanger (160) temperature is realized Degree adjust, wherein, the circulation of fluid in the heat exchanger with heat transport fluid heat-shift, the heat transport fluid for example for Reheat the ball (170) of boiler or health hot water.Therefore, destination apparatus of the invention make it possible to adjust and are provided with thereon originally The active thermal conditioning of the building (100) of the destination apparatus of invention.
Fig. 2, the implementation example of destination apparatus of the invention, cellular board includes the first plate with big honeycomb (111), Big honeycomb (111) constitutes the pipeline for culture medium circulation.The plate be clipped in more cellulor two cellular boards (212, 213) between, in less honeycomb, the circuit cycle that colored fluid is separated by the closed circuit with culture medium.Therefore, According in the plate for being referred to as adjusting, (212, the color of the fluid circulated in honeycomb 213), the response of light energy device is changed.Root According to implementation example, opaque fluid circulation in the adjustable plate of light energy (212) is directly exposed to, this makes culture medium lose this Light, limits and reheats and stopped photosynthesis.According to another implementation example, the honeycomb being fixed on outer surface (120) The circulation of the dark liquid in plate (213) make it possible to increase light energy absorption and therefore increase culture medium temperature.
Microalgae is mixotrophic, and its behavior is determined by lighting condition.Therefore, light is lost, the microalgae is using different Foster behavior, and light is exposed to, it adopts autotrophy behavior.Therefore, by change adjustable plate (212,213) in circulation of fluid light Characteristic is learned, the behavior of microalgae is have modified, this is advantageously used in makes the behaviour adaptation of the microalgae be present in Cyclic culture medium Nutrients characteristic.
Fig. 3, the culture medium for regularly preparing is injected in pipeline.The culture medium is by microalgae and aqueous mixture Three types composition mixing.According to first embodiment, the microalgae is tied with the effluent (311) for including mineral nutrition thing Close.This effluent is especially to be digested in advance by the bacterium of consumption organic matter.As an example, the effluent is the leaching discharged Filtrate, methanation biogas residue and biogas liquid or building sewage.The effluent is combined with carbon dioxide (312).The embodiment is more specifically It is suitable to produce microalgae by preferentially realizing the autotrophy behavior of the microalgae.The generation pattern makes it possible to reach per liter of a few gram quantity The microalgae concentration of level.On the other hand, the embodiment is caused in the unusable effluent increment of other field.
According to another embodiment, effluent (313) is including organic nutrient substance.The embodiment advantageously uses food organic waste The waste unworthy in other areas of thing, such as cannery, slaughterhouse or restaurant.The embodiment is by the way that microalgae is protected Exist at lucifuge to use the heterotrophism behavior of microalgae.The embodiment allows to produce per liter hundred microalgae for carrying out gram quantity level.
Characteristic regardless of the effluent for being used, effluent in appropriate device (350) by micronized, to increase Exchange surface between microalgae and the nutrients.File US2009 0029445 describes the example of atomization process.
Fig. 4, regularly extracts a certain amount of culture medium to extract microalgae.Culture medium when in culture medium microalgae it is dense When degree with reaction more than corresponding threshold value is slowed down, the extraction is realized according to the concentration.Advantageously, fluid passes through adaptive device And undergo flocculation operation (450).The flocculation operation makes it possible to promote to extract the microalgae, and the wherein microalgae is greatly reduced Size does not allow to use membrane separation technique or Direct Filtration Technology, unless disappeared than reclaiming the microalgae for the filter operation Consumption more multi-energy.Therefore, methods described (450) makes it possible to separate to prepare with the water (414) reused by the microalgae Culture medium.Microalgae (415) is dispatched in the value-added field of energy, such as preparing biodiesel.
Advantageously, by means of making culture medium undergo the method for electric pulse to realize flocculation process.This method for example exists Described in file WO2012 054404.
Above description and implementation example show to invention achieves expected purpose, and especially, it leads to according to positive energy process Cross the thermal conditioning of realizing building and cause the increment of contaminative effluent.

Claims (11)

1. a kind of device, including:
I. the first transparent honeycomb plate (110) that can be fixed on external surface of buildings (120), and for including microalgae The device (150) of circulation of the culture medium in pipeline (111), the pipeline (111) is including the first transparent honeycomb plate Honeycomb;
Wherein, the first transparent honeycomb plate be clipped in it is following between the two:
Ii. it is fixed on the second transparent honeycomb plate (212) being exposed on the face of light of the first transparent honeycomb plate, described Two transparent honeycomb plates include the colored fluid in the honeycomb of the second transparent honeycomb plate for separating with the culture medium Circulation device;
Iii. it is fixed on the first transparent honeycomb plate on the face relative with the stationary plane of the second transparent honeycomb plate The 3rd transparent honeycomb plate (213), the 3rd transparent honeycomb plate include for separate with the culture medium the described 3rd The device of the circulation of the colored fluid in the honeycomb of transparent honeycomb plate.
2. device according to claim 1, also includes:
Iv. the heat exchanger between the culture medium for circulating in the honeycomb of the first transparent honeycomb plate and heat transport fluid (160)。
3. device according to claim 2, wherein, the heat transport fluid is to circulate in the second transparent honeycomb plate Colored fluid or the colored fluid circulated in the 3rd transparent honeycomb plate.
4. it is a kind of require the device described in 1 for usage right process effluent (311, method 313), it is characterised in that The method comprising the steps of:
A. the aqueous culture medium for including mixotrophism microalgae, carbon dioxide, organic nutrient substance and mineral nutrition thing is prepared;
B. culture medium injection is included into that the pipeline (111) of the first transparent honeycomb plate (110) is exposed to closing for light Close loop (135) in, and by making the second transparent honeycomb plate in or the 3rd transparent honeycomb plate in appropriate color Fluid circulation controlling the culture medium light intensity that the microalgae is undergone during circulation in the pipeline (111) Degree, and comprise the following steps:
Bii. exposure of the culture medium to light is reduced, to be conducive to disappearing for organic matter described in the heterotrophism behavior of the microalgae Consumption;
Biii. increase the exposure to light of the culture medium, to be conducive to the autotrophy behavior of the microalgae, and be conducive to institute State digestion and the photosynthesis of mineral matter in effluent;
C. the concentration of the microalgae in the culture medium of circulation is measured;
D. when the concentration of the microalgae reaches threshold value, a certain amount of Cyclic culture medium is harvested;
E. water (414) and the microalgae (415) are separated from the Cyclic culture medium of the amount;
F. the microalgae (415) for reclaiming is used as energy source.
5. method according to claim 4, wherein, step a) is comprised the following steps:
Ai. effluent micronized (350) is made in the aqueous solution.
6. method according to claim 4, wherein, the effluent (311) is lixivium.
7. method according to claim 4, wherein, the effluent (311) is methanation biogas residue and biogas liquid.
8. method according to claim 4, wherein, the effluent (311,313) it is made up of the sewage of building.
9. method according to claim 4, wherein, step d) is comprised the following steps:
Di. flocculation (450) is included in the microalgae in the culture medium.
10. method according to claim 9, wherein, step di) it is by making the culture medium undergo electric pulse come real Existing.
11. methods according to claim 4, wherein, the effluent (313) is realized from food waste.
CN201380004180.4A 2012-03-12 2013-03-12 Positive-energy photobioreactor device and method using such a photobioreactor Expired - Fee Related CN104066832B (en)

Applications Claiming Priority (3)

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FR1252199A FR2987851B3 (en) 2012-03-12 2012-03-12 BUILDING FACADE ACTIVE INSULATION SYSTEM AND ASSOCIATED MODULAR ISOLATION PANEL
FR1252199 2012-03-12
PCT/EP2013/055055 WO2013135736A1 (en) 2012-03-12 2013-03-12 Positive-energy photobioreactor device and method using such a photobioreactor

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CN104328029A (en) * 2014-10-30 2015-02-04 国家开发投资公司 Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method
ITUB20153720A1 (en) * 2015-09-18 2015-12-18 Torino Politecnico MODULAR ELEMENT, SYSTEM AND PROCESS OF TREATMENT OF WASTE AND METEORIC WATERS.

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FR2987851B3 (en) 2014-08-08
WO2013135736A1 (en) 2013-09-19

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