CN104328106B - Microcapsule preparation apparatus and method - Google Patents

Abstract

The invention provides a microcapsule preparation apparatus. The microcapsule preparation apparatus is composed of an injection unit and an evenly-mixing power unit; wherein the injection unit comprises a composite injector, a tray, a voltage generating unit, an elevating rod, and a clamp; the elevating rod is vertically fixed on the tray, a clamp, which can move up and down, is arranged on the elevating rod, the injector body is a cylindrical container, a bypass is arranged on the device wall, the other end of the bypass is inserted into the injector; the voltage generating unit comprises a positive electrode connection rod, a negative electrode connection terminal, and a positive electrode connection terminal; and the evenly-mixing power unit comprises a Stirling power device and a hand-operated device. The provided microcapsule microsphere combined with the provided experiment conditions has the characteristics of small volume, round and uniform shape, and good tissue compatibility. In order to control the voltage and pneumatic piston movement, a high voltage electrostatic field generator and a microscale liquid pump can be provided in addition. The provided device has the advantages of low cost, user-friendliness, and small volume, and can be used in an ultra-clean workbench.

Description

A kind of microcapsule preparation facilitiess and the method preparing microcapsule

Technical field

A kind of the invention belongs to petroleum catalytic cracking field, in particular it relates to Temperature Distribution of the catalyst being hydrocracked Acquisition methods.

Background technology

Microencapsulation belongs to one kind of immobilization technology, its essence is a kind of made semipermeable membrane, enzyme, coenzyme, egg The biomacromolecules such as white matter or animal and plant cellss wrap up wherein.Microcapsule membrane allows the small-molecule substance by below 100ku, capsule Interior cell can carry out mass exchange through microcapsule membrane with the external world, obtain nutrition and discharge metabolic waste, and macromolecule Immunoglobulin can not pass through, thus improving cell density and product assay, and facilitates separation and purification treatment, it is to avoid immunity is damaged Wound.

The method preparing microcapsule mainly uses polyelectrolyte complex principle, with polyanion and the poly- sun of oppositely charged Ionic reaction, in physiological conditions in being coated formation peplos around thing, preparation method mainly includes air-atomizing encystation method and height Pressure electrostatic encystation method.Islet cellss are wrapped in sodium alginate by lim in 1980 etc. first, gather and rely in ammonia intoxicated polyethyleneimine film, It is implanted into experimental diabetic rats intraperitoneal, maintain euglycemia to reach 3 weeks, experiment demonstrates the immune buffer action of microcapsule, It protects graft from immunity of organism rejection, and extends the survival of external source islets of langerhans and function is held time.This is real Test the breakthrough bringing initiative to islet transplantation research, so that islet transplantation technology enters new epoch.

Currently, the domestic personnel being engaged in microencapsulation research direction there is no a kind of special, convenient microencapsulation apparatus for preparation, It mostly is homemade simple instrument, this design mainly has several shortcomings: 1. be difficult to essence taking patent cn 1566339a as a example Really regulation and control needle point, to liquid level, controls the height condition of reaction.2. each use is required for lengthy and tedious number of assembling steps, such as changes Syringe etc..3. microinfusion pump directly put with response location more than, there is potential safety hazard.4. reaction vessel no solution mixes Function, the microcapsules and microsphere produced is susceptible to pile up and leads to stick into group, wastes valuable experiment material.

Content of the invention

The weak point existing for this area, the purpose of the present invention is to propose to a kind of microcapsule preparation facilitiess.

It is another object of the present invention to proposing a kind of method preparing microcapsule.

The technical scheme realizing the object of the invention is:

A kind of microcapsule preparation facilitiess, by injecting unit and mix power unit form, injecting unit and mix power unit It is each attached in rectangular base；

Described injecting unit includes composite injection device, pallet, voltage generating unit, elevating lever and fixture；On described pallet Vertically fix elevating lever, elevating lever is provided with fixture moving up and down, described composite injection device passes through described fixture and consolidates Determine elevating lever to connect；

Described composite injection device includes main body syringe, input syringe, needle assembly, bipass；Described needle assembly It is fixed on needle mount syringe needle in parallel including three, described main body syringe is column shape container, and wall is provided with bipass, The other end insertion input syringe of bipass；

Described voltage generating unit includes anelectrode pitman, negative electrode incoming end, anelectrode incoming end；Described negative electrode Incoming end and anelectrode incoming end are connected by wire, and described anelectrode pitman is by the wire within needle mount and syringe needle Connect；The end of described negative electrode incoming end is metal bar, is connected with pallet by switch module；

Described mixing power unit includes CETRINE power set and hand gear；Described CETRINE power set include justifying Cylindricality glass outer, the piston being placed in glass outer and alcohol burner, described piston is connected with the first driven rod；Described manual dress Be set to can hand rotation handwheel, described handwheel connects the second driven rod；Described first driven rod and the second driven rod all with support Disk connects.

Wherein, input syringe is conventional universal syringe, is responsible for interim receiving microencapsulation object enzyme, coenzyme, egg The biomacromolecules such as white matter or animal and plant cellss.And, needle assembly is designed for Detachable with the bottom of composite injection device, can lead to That crosses bottom outlet and ditch siphunculus agrees with the bottom being temporarily fixedly connected on composite injection device.

Wherein, described fixture is fixed with tube for transfusion, strap, for adjusting the upper and lower spiral micro actuator of fixture and use In folder anelectrode pitman electricity folder.

Described strap is provided with four variable folders, and described composite injection device is fixed in the middle of four variable folders.

Wherein, described main body syringe is column shape container, and there is piston inside, and piston is sequentially connected with piston push rod and manually Pull bar；Described piston has the region moving up and down in column shape container；

Described main body syringe top has a insertion sheath and block that can open of tube for transfusion.

Preferably, the bottom of fixture is ' t ' shape height gauge, and the bottom of ruler is high together with three syringe needles fixing；Lifting There is rule the lower section of bar, is subtracted each other by scale, can conveniently calculate the height of liquid level and needle point.Further, height gauge is located at The positive front lower place in bottom of fixture.

Wherein, the end of described negative electrode incoming end is metal bar, exports pin by switch module and negative electrode and is connected, institute State negative electrode output pin syringe needle and pallet spacing 1-3mm.(syringe needle is not contacted with pallet, because culture dish to be put on pallet, There is the space of 1-3mm) between syringe needle and pallet.

Preferably, described rectangular base is additionally provided with the discharge cock for closing voltage of electric field.

Wherein, the cylindrical glass outer housing of described CETRINE power set is provided with radiator near one end of pallet；Institute Be provided with outside the second driven rod stating handwheel connection radiating outer housing (, connect free between cylindrical glass outer housing and radiating outer housing Heart pipe.Because the radiating outer housing of hand gear is the secondary heat abstractor of radiator, in order to improving heat radiation efficiency and pneumatic efficiency, The scaled down version of CETRINE power unit can be regarded as, simply do not add alcohol burner.If manual power unit also adds ethanol If lamp, do not allow for being connected to hollow pipe between two power units, because the section of the cold and hot conversion of gas can be interfered with each other Rule is so that the efficiency of energy conversion reduces.So, connecting two devices with hollow pipe is to reach gas in two devices Between flowing purpose.So this hollow pipe need be thinner pipe, and the material of hollow pipe can be flexible plastic or metal is equal Can.

Described tray bottom is uniformly distributed four around bearing, is placed on basetray (10)；Described cincture bearing bag Include disk, middle disk, lower disc, upper bearing (metal), lower bearing；Upper disk, middle disk, the edge of lower disc is connected by bearing (note: upper disk 46 is fixedly connected with middle disk, and middle disk is fixedly connected upper bearing (metal), but upper bearing (metal) and upper disk are non-solid Fixed connect, but can with the axle center of upper bearing (metal) as axle, mutual turn)；Four cincture bearings positioned at tray bottom are same The each two of side is one group around bearing, is connected with belt.

A kind of method preparing microcapsule, using device of the present invention, including step:

1) culture dish that will be equipped with calcium chloride solution is placed on pallet, puts down negative electrode output pin end, confirms needle point insertion Liquid level the center away from culture dish, in case there is viscous sticking in the microsphere of microencapsulation.

2) three syringe needles in parallel are adjusted between calcium chloride liquid level 9mm～21mm；

3) draw the sodium alginate that the concentration mixing is 2% and the mixture of cell with input syringe, then will inject Device inserts the interface of bipass；

4) open the first bipass, push injection device afterbody makes sodium alginate mixture input main body syringe, and piston is It is pushed upwardly therewith.

5) use alcohol burner Preheat glass outer housing.Before shaking table starts formally to operate, should ensure that at least preheating 1min, preferably 1-5min.

6) open block, tube for transfusion injects liquid to the top of main body syringe, as hydraulic power.Treat that liquid will be filled with During the top cavity volume of main body syringe, close block, stop bolus injection.

7) manual toggle pallet, in the presence of CETRINE power set, pallet starts shaking table motion.

8) open the second bipass, discharge cock, and the transfusion speed of microinfusion pump is controlled in 10-15ml/h, pin Head starts to export sodium alginate mixture.

First bipass and the second bipass are the different bipass of effect, and the first bipass is responsible for preventing main body syringe Interior material operationally blows back in universal syringe；And the second bipass 22 is responsible for preventing universal syringe from noting to main body In emitter during injection sodium alginate (step 4), sodium alginate is just surprisingly flowed out by three syringe needles, and now device is not also Enter working condition.

Preferably, the voltage between described anelectrode pitman and negative electrode output pin is 4000v～11000v.

When lacking alcohol burner under experiment condition, can be in step 7) in manually shake handwheel there is shaking table motion.

The rotating speed of coarse adjustment shaking table can be carried out by debugging the distance between alcohol burner flame and glass outer.

The inventive method has the advantage that

The microcapsules and microsphere that the present invention produces combines the experiment condition that the present invention provides, and has a small volume, form circle and Uniform feature is good with the compatibility of tissue.For control voltage and hydraulic piston movement, can additionally purchase high-voltage electrostatic field and send out Raw device and microinfusion pump.Assembly of the invention low cost, easy to use.Whole device small volume, can be in superclean bench Interior use.

The fixture of the application can be for the characteristic of different size of microsphere or capsulating material, and accuracy controlling needle point is to liquid level Highly；Change capsulating material or biomaterial it is not necessary to re-assembly, change syringe convenient；With CETRINE power or hand Power provides solution to mix function, and the shake speed of pallet is controlled, more more stable than the shaking apparatus of prior art, speed is adjustable.

Brief description

Fig. 1 is microcapsule preparation facilitiess axonometric chart of the present invention.

Fig. 2 is the microcapsule preparation facilitiess axonometric chart not placing injecting unit.

Fig. 3 is injecting unit axonometric chart.

Fig. 4 is needle assembly axonometric chart.

Fig. 5 is microcapsule preparation facilitiess base partial view.

Fig. 6 is the front view of CETRINE power set.

Fig. 7 be Fig. 6 in pallet remove after top view.

Fig. 8 is to mix power unit partial sectional view.

Fig. 9 is the microcapsule photo that embodiment 2 is obtained.

Figure 10 is the microcapsule photo that example 3 is obtained.

Figure 11 is multiple microcapsule photos that example 3 is obtained.

In figure, component names and numbering for relation are:

Composite injection device 1, base 2, pallet 3, mix power unit 4, main body syringe 5, input syringe 6, syringe needle group Part 7, fixture 8, elevating lever 9, base body 10, tray main body 11, negative electrode exports pin 12, around bearing 13, syringe needle 14, syringe needle Base 15, plastic safety cover 16, anelectrode pitman 17, negative electrode incoming end 18, anelectrode incoming end 19, discharge cock 20, First bipass 21, the second bipass 22, ditch siphunculus 23, block 24, manual draw bar 25, inserts sheath 26, fixed column 27, piston pushes away Bar 28, piston 29, tube for transfusion 30, microinfusion pump extension tube 31, bottom outlet 34, ditch siphunculus 35, strap 36, spiral micro actuator 37, electricity folder 38, variable folder 39, height gauge 40, rule 41, plug 42, arc-shaped conductive film 44, metal bar 45, upper disk 46, in Disk 47, lower disc 48, upper bearing (metal) 49, lower bearing 50, lower disc 51, belt 52, the first driven rod 53, the first driven rod connecting rod 54, radiator 55, Stirling device piston 56, glass outer 57, Stirling device support column 58, alcohol burner 59, sealing shroud 60, Second driven rod 61, the piston 62 of hand gear, radiate outer housing 63, handwheel connecting rod 64, handwheel 65, hand gear support column 66, Hand gear sealing shroud 67, spill wheel shaft 68, flexible plastic hollow pipe 69.

Specific embodiment

Following examples are used for the present invention is described, but are not limited to the scope of the present invention.

Unless stated otherwise, the technology used in the present invention means, are this area conventional technique means.

Embodiment 1 microcapsule preparation facilitiess

Referring to Fig. 1 and Fig. 2, the present embodiment microcapsule preparation facilitiess, it is made up of injecting unit and mixing power unit 4, injection Unit and mixing power unit are each attached in rectangular base 2.Described injecting unit includes composite injection device 1, pallet 3, voltage Generation unit, elevating lever 9 and fixture 8；Vertically fix elevating lever 9 on described pallet 3, elevating lever 9 is provided with and can move up and down Fixture, described composite injection device 1 pass through described fixture with fixation elevating lever 9 be connected；

Referring to Fig. 3.Described composite injection device 1 includes main body syringe 5, input syringe 6, needle assembly 7, bipass； Described needle assembly 7 includes three syringe needles 14 being fixed on needle mount 15 parallel connection, and described main body syringe 5 is column shape container, First bipass 21 and the second bipass 22, the other end insertion input syringe 6 of bipass are provided with wall.Bipass 21 Inside be a solid sphere, the long axis direction that spheroid is located in ditch siphunculus 23 has a hollow cylinder, has communication input and injects Device 6 and the function of main body syringe 5.When bipass 21 rotates, hollow cylinder staggers the major axis of ditch siphunculus 23, inputs syringe 6 Pipeline is closed and main body syringe 5 between.There is bipass 22 in the bottom side of main body syringe, it constructs and bipass 21 Unanimously, there is the effect opening or closing the fluid passage between main body syringe 5 and needle assembly 7.Bipass shown in figure 21 are contacted and force direction of rotation for finger with the black full-filling part on bipass 22.

Described voltage generating unit includes anelectrode pitman 17, negative electrode incoming end 18 anelectrode incoming end 19；Described Negative electrode incoming end 18 and anelectrode incoming end 19 are connected by wire, and described anelectrode pitman 17 passes through in needle mount 15 The wire in portion is connected with syringe needle 14；The end of described negative electrode incoming end 18 is metal bar 45, by switch module with pallet 3 even Connect (pallet 3 is located at tray main body 11 surface)；

This mixing power unit 4 includes CETRINE power set and hand gear；Described CETRINE power set include justifying Cylindricality glass outer 57, the piston 56 being placed in glass outer 57 and alcohol burner 59, described piston 56 and the first driven rod connecting rod 54 and first driven rod 53 connect；Described hand gear be with support column 66 support can hand rotation handwheel 65, include biography Lever 64, handwheel 65, support column 66, sealing shroud 67, spill wheel shaft 68.The outer rim setting handwheel connecting rod 64 of handwheel, handwheel 65 leads to Cross handwheel connecting rod 64 and connect the second driven rod 61 (being connected in the spill wheel shaft 68 of handwheel wheel shaft outer ring)；Second drive link 61 connects lives Plug 62 (see Fig. 8), have radiating outer housing 63, described first driven rod 53 and the second driven rod 61 to be all connected with pallet 3 outside piston 62. The setting of hand gear sealing shroud 67 and effect are with Stirling device sealing shroud 60.

Tube for transfusion 30 is fixed with fixture 8, strap 36, for adjusting spiral micro actuator 37 about 8 for the fixture and using In folder anelectrode pitman 17 electricity folder 38.Described strap 36 is provided with four variable folders 39, and insertion fixed column 27 will be described Composite injection device 1 is fixed in the middle of four variable folders 39.

Main body syringe 5 is column shape container, and there is piston 29 inside, piston is sequentially connected with piston push rod 28 and manual draw bar 25；Piston 29 has the region moving up and down in column shape container；

Main body syringe 5 top has a insertion sheath 26 and block that can open 24 of tube for transfusion 30.With lifting dress Put 8 tube for transfusions 30 being fixedly linked and can be inserted into insertion sheath 26.When microinfusion pump starts first, first open block 24, then Make liquid as motive force by the supporting quick back inlet from combined lifting device 8 of microinfusion pump extension tube 31 Enter tube for transfusion 30, and then the top reservoir space by feed liquor sheath 26 entrance main body syringe 5, when liquid will be filled with reservoir sky Between when, stop microinfusion pump and simultaneously close upper cap (24), be that ensuing microcapsules and microsphere of formally preparing is prepared.See Fig. 4, three The distributing position of syringe needle is three syringe needles 14 in parallel and the anelectrode pitman on the summit occupying an equilateral triangle respectively 17 metallic circuits passing through within needle mount 15 are connected, and syringe needle 14 is passed through internal path and communicated with bottom outlet 34.Transparent moulds Material protective cover 16 as insulation protection device annular around around needle mount 15, bottom level slightly higher with syringe needle 14.And, Needle assembly 7 is designed for Detachable with the bottom of composite injection device 1, temporarily can be fixed with agreeing with of ditch siphunculus 35 by bottom outlet 34 It is connected to the bottom of composite injection device 1.

In the present embodiment, the positive front lower place in bottom of fixture 8 is ' t ' shape height gauge 40, the bottom of ruler and three fixing The connection neat height of syringe needle 14；There is rule 41 lower section of elevating lever 9, is subtracted each other by scale, can conveniently calculate the height of liquid level and needle point.

The end of negative electrode incoming end 18 is metal bar 45, exports pin 12 by switch module and negative electrode and is connected, negative electricity Pole exports the space having 2mm between pin 12 syringe needle and pallet 3.It is provided with high-pressure electrostatic field generator on rear side of the upper left corner of base 10 to connect Negative electrode incoming end 18 and the anelectrode incoming end 19 of roll line.Wherein, negative electrode incoming end 18 passes through internal wiring and plug 42 It is connected.Switch 20, as the discharge cock of high-voltage electrostatic field, when switch is allocated to left side, has resilient arc-shaped conductive film 44 Separate with metal bar 45, the high-voltage electrostatic field between needle point and liquid level disappears.It is additionally provided with for closing electric field in rectangular base 2 The discharge cock 20 of voltage.Base is located at the surface of base body 10.When switching 20 and being allocated to right side, conducting strip and metal bar phase Engage, (referring to Fig. 5) is recovered in the high-voltage electrostatic field between needle point and liquid level.

See Fig. 6 and Fig. 7.The cylindrical glass outer housing 57 of CETRINE power set is provided with radiating near one end of pallet 3 Device 55；Wherein, the first drive link 53 pass through i.e. can sealing gas, can smoothly make the CETRINE that the first drive link 53 slides again Device sealing shroud 60.Have between piston 56 and glass outer 57 can supplied gas flowing slight void, radiator 55 is that annular is folded Plus metal fin, have between fin space be available for radiate, be fixed on the outside of glass outer 57.

This simple CETRINE of single cylinder type mixes the motion ultimate principle of power set: the inside of whole power set is closure Space, but leave space between the inwall of piston 56 and glass outer 57 and can supplied gas move, hot gas available hydrogen, helium Gas, nitrogen or air etc..First drive link 53 is fixedly linked through sealing shroud 60 with piston 56, and piston can be in glass outer 57 Inwall is slidably.When alcohol burner heating enclosure, as the hot gas expanded by heating of working medium, in sufficiently long preheating time After, Manual-pushing main body pallet rotates, if piston shifts to fire end (i.e. alcohol burner place end) first, heated gas are to cold But hold at mobile (i.e. radiator place end), gas cooling after-contraction, and then drive the air of fire end to move to colling end, simultaneously The hot gas expanding also has the promotion trend to fire end movement for the piston.When the first drive link 53 moves to distalmost end or nearest During end, and turn over farthest end points or nearest end points under the inertia of wheel disc drives, piston shifts to colling end.Now due to cold But the air held cools down, and the amount of the air of fire end occurs relatively to reduce, and in the presence of negative pressure, piston continues to inhale To colling end, there is expanded by heating in the cold air flowing to fire end again, promotes piston to shift to colling end further.Therefore, as long as The heating of alcohol burner does not stop, and CETRINE power set will produce endlessly power, and piston will circulate with drive link Reciprocal contraction.

It is provided with radiating outer housing 63, cylindrical glass outer housing 57 and radiating outside the second driven rod 61 that described handwheel 65 connects It is connected with flexible plastic hollow pipe 69, in order to improving heat radiation efficiency and pneumatic efficiency between outer housing 63.

Described tray main body 11 bottom even is distributed four around bearing 13, is placed on basetray 10；Described cincture Bearing 13 includes disk 46, middle disk 47, lower disc 48, upper bearing (metal) 49, lower bearing 50.Upper disk 46, middle disk 47, lower circle The edge of disk 48 (particularly as follows: upper disk 46 is fixedly connected with middle disk 47, middle disk is fixedly connected upper shaft by bearing connection Hold 49, but upper bearing (metal) 49 is connected for on-fixed with upper disk 46, but can be with the axle center of upper bearing (metal) 49 as axle, mutual turn )；Four each two around bearing in the same side positioned at pallet 3 bottom are one group around bearing, are connected with belt 52.

Embodiment 2

The method the present embodiment preparing microcapsule so that high-voltage electrostatic field method prepares sodium alginate micro-capsule as a example, in 25 DEG C of room temperature, Under the experiment condition of medial humidity 60%～70%, preparation average diameter is 1000 μm of microsphere.

Using the device of embodiment 1, including step:

1) culture dish that will be equipped with calcium chloride solution is placed on pallet 3, puts down negative electrode output pin end 12, confirms that needle point is inserted Enter liquid level the center away from culture dish, in case the microsphere of microencapsulation occurs viscous sticking.

2) three syringe needles in parallel (14) are adjusted between calcium chloride liquid level 9mm～11mm；

3) draw the sodium alginate that the concentration mixing is 2% and the mixture of cell with input syringe 6, then will inject Device inserts the interface of bipass 21；

4) open the first bipass 21, push injection device afterbody makes sodium alginate mixture input main body syringe 5, piston 29 are pushed upwardly therewith.

5) use alcohol burner 59 Preheat glass outer housing 57.Before shaking table starts formally to operate, should ensure that at least preheating 1min.

6) open block 24, tube for transfusion 30 injects liquid to the top of main body syringe 5, as hydraulic power.Treat liquid Will be filled with main body syringe 5 top cavity volume when, close block 24, stop bolus injection.

7) manual toggle pallet 3, in the presence of CETRINE power set, pallet 3 starts shaking table motion.

8) (the first bipass 21 is responsible for preventing the material in main body syringe from operationally flowing backwards to open the second bipass 22 Enter in universal syringe；Second bipass 22 is responsible for preventing step 4) universal syringe into main body syringe inject alginic acid During sodium, sodium alginate is just surprisingly flowed out by three syringe needles), discharge cock 20, and the transfusion speed of microinfusion pump is controlled In 10-15ml/h, syringe needle (14) starts to export sodium alginate mixture.

Voltage between anelectrode pitman (17) and negative electrode output pin (12) is 5500v.Prepared microcapsule photo is shown in Fig. 9.

Embodiment 3

The present embodiment so that high-voltage electrostatic field method prepares sodium alginate micro-capsule as a example, in 25 DEG C of room temperature, medial humidity 60%～ Under 70% experiment condition, preparation average diameter is 10 μm of microsphere.

Operating procedure with example 2, experiment condition is:

1. three syringe needles are 19～21mm to the distance of liquid level.

2. voltage is about 11000v.

3. should ensure that ' phenomenon of bursting apart ' in drop.Phenomenon is: operator's direct-view drop, and drop is departing from a needle point 3mm left side The imagination suddenly disappearing occurs behind the right side, it is substantially charged drop and is torn by powerful electric field force and exists for countless diameters 10um about the sightless small microsphere of naked eyes phenomenon.About 8800v about voltage be this phenomenon critical point, drop After falling, visually can may be immediately observed that the fluid film that ' umbrella ' or ' herringbone ' is diverged to.But prepare under this voltage Microcapsule the adhesion of a large amount of fibrous bands samples occurs and get together it is impossible to be used for scientific research.

Figure 10, the 11 microcapsule photos being obtained for the present embodiment.

Although, above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.

Claims (7)

1. a kind of microcapsule preparation facilitiess it is characterised in that by injecting unit and mix power unit (4) form, injecting unit with Mix power unit to be each attached in rectangular base (2)；

Described injecting unit includes composite injection device (1), pallet (3), voltage generating unit, elevating lever (9) and fixture (8)；Institute State and vertically fix elevating lever (9) on pallet (3), elevating lever (9) is provided with fixture moving up and down, described composite injection device (1) pass through described fixture to be connected with fixing elevating lever (9)；Wherein, the bottom of fixture (8) is ' t ' shape height gauge (40), ruler Bottom is high together with three syringe needles (14) fixing；There is rule (41) lower section of elevating lever (9)；

Described composite injection device (1) includes main body syringe (5), input syringe (6), needle assembly (7), bipass；Described Needle assembly (7) includes three and is fixed on needle mount (15) syringe needle (14) in parallel, and described main body syringe (5) is held for column Device, wall is provided with the first bipass (21) and the second bipass (22), the other end insertion input syringe of bipass (6)；

Described voltage generating unit includes anelectrode pitman (17), negative electrode incoming end (18), anelectrode incoming end (19)；Institute State negative electrode incoming end (18) and anelectrode incoming end (19) is connected by wire, described anelectrode pitman (17) passes through syringe needle The internal wire of base (15) is connected with syringe needle (14)；The end of described negative electrode incoming end (18) is metal bar (45), passes through Switch module is connected with pallet (3), and is connected by switch module and negative electrode output pin (12), and described negative electrode exports pin (12) syringe needle and pallet (3) spacing 1-3mm；

Wherein, described rectangular base (2) is additionally provided with the discharge cock (20) for closing voltage of electric field；

Described mixing power unit (4) includes CETRINE power set and hand gear；Described CETRINE power set include justifying Cylindricality glass outer (57), the piston (56) being placed in glass outer (57) and alcohol burner (59), described piston (56) and first Driven rod (53) connects；Described hand gear be can hand rotation handwheel (65), described handwheel (65) connects the second driven rod (61)；Described first driven rod (53) and the second driven rod (61) are all connected with pallet (3)；The circle of described CETRINE power set Cylindricality glass outer (57) is provided with radiator (55) near one end of pallet (3)；The second transmission that described handwheel (65) connects It is provided with radiating outer housing (63) outside bar (61), between cylindrical glass outer housing (57) and radiating outer housing (63), be connected with hollow pipe.

2. microcapsule preparation facilitiess according to claim 1 are it is characterised in that be fixed with tube for transfusion on described fixture (8) (30), strap (36), for adjusting the upper and lower spiral micro actuator (37) of fixture (8) and being used for pressing from both sides anelectrode pitman (17) Electricity folder (38)；

Described strap (36) is provided with four variable folders (39), and described composite injection device (1) is fixed on four variable folders (39) in the middle of.

3. microcapsule preparation facilitiess according to claim 1 it is characterised in that described main body syringe (5) be column shape container, There is piston (29) inside, and piston is sequentially connected with piston push rod (28) and manual draw bar (25)；Described piston (29) is held in column There is the region moving up and down in device；

Described main body syringe (5) top has the insertion sheath (26) of tube for transfusion (30) and a block that can open (24).

4. according to the arbitrary described microcapsule preparation facilitiess of claim 1-3 it is characterised in that described pallet (3) bottom even divides Cloth four, around bearing (13), is placed on basetray (10)；Described disk (46) in bearing (13) inclusion, middle disk (47), lower disc (48), upper bearing (metal) (49), lower bearing (50)；Upper disk (46) and middle disk (47) are connected by bearing, middle circle Disk (47) and lower disc (48) are connected by bearing；Four cincture each two in the same side for the bearing positioned at pallet (3) bottom It is one group around bearing, connected with belt (52).

5. a kind of method preparing microcapsule is it is characterised in that adopt the arbitrary described device of claim 1-4, including step:

1) culture dish that will be equipped with calcium chloride solution is placed on pallet (3), puts down negative electrode output pin (12), makes needle point insert liquid Face the center away from culture dish；

2) three syringe needles in parallel (14) are adjusted between calcium chloride liquid level 9mm～21mm；

3) draw the mixture of the sodium alginate that the concentration mixing is 2% and cell with input syringe (6), then by syringe The interface of insertion bipass (21)；

4) open the first bipass (21), push injection device afterbody makes sodium alginate mixture input main body syringe (5), piston (29) it is pushed upwardly therewith；

5) use alcohol burner (59) Preheat glass outer housing (57), preheat 1-5min before shaking table starts formally to operate；

6) open block (24), tube for transfusion (30) injects liquid to the top of main body syringe (5), as hydraulic power；

7) manual toggle pallet (3), in the presence of CETRINE power set, pallet (3) starts shaking table motion；

8) open the second bipass (22), discharge cock (20), and the transfusion speed of microinfusion pump is controlled in 10-15ml/ H, syringe needle (14) starts to export sodium alginate mixture.

6. method according to claim 5 is it is characterised in that described anelectrode pitman (17) exports pin with negative electrode (12) voltage between is 4000v～11000v.

7. method according to claim 5 is it is characterised in that described step 7) in manually shake handwheel (65) there is shaking table Motion.