CN105267991A - Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method - Google Patents
Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method Download PDFInfo
- Publication number
- CN105267991A CN105267991A CN201510736204.6A CN201510736204A CN105267991A CN 105267991 A CN105267991 A CN 105267991A CN 201510736204 A CN201510736204 A CN 201510736204A CN 105267991 A CN105267991 A CN 105267991A
- Authority
- CN
- China
- Prior art keywords
- quantum dot
- microcapsule
- graphene quantum
- oil
- drug
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicinal Preparation (AREA)
- Manufacturing Of Micro-Capsules (AREA)
Abstract
The invention relates to a microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method. A drug-loading microcapsule using a cross-linking film containing graphene quantum dots as a capsule wall and an oil phase loading a hydrophobic drug as a core is formed by: performing ultrasonic radiation on an aqueous solution obtained by modification with an organic polymer or biopolymer containing both sulfhydryl groups and amino groups and on the oil phase loading the hydrophobic drug. The graphene quantum dots are evenly distributed on the capsule wall of the microcapsule and are modified with the organic polymer or biopolymer, spacing among the graphene quantum dots is enlarged, and fluorescent tracing performance of the microcapsule during drug delivery is guaranteed; the formed capsule wall containing the graphene quantum dots encapsulates and protects a drug; drug release is effectively controlled by using reduction responsiveness of disulfide bonds; the preparation method is quick and simple and efficient and environment-friendly, and products are pure and nontoxic.
Description
Technical field:
The present invention relates to a kind of bio-medical material, be specifically related to a kind of cyst wall and contain there is fluorescent tracing and reducing response medicine release function microcapsule and preparation method of graphene quantum dot.
Background technology:
Microcapsule is a kind of miniature vessel or packing material with membranaceous wall shell, due to the particularity of its structure and the polytropy of performance, is all widely used in fields such as biological medicine, food and agriculturals.Can be used for material prepared by microcapsule varied, mainly comprise the macromolecular material and inorganic compound etc. of natural macromolecular material, synthesis.The preparation method of microcapsule has a variety of, according to encystation mechanism, can be roughly divided into three classes: Physical, physical-chemical process and chemical method.Along with the development of scientific research, continue to bring out out the technology of some new micro encapsulation, as surface grafting, matrix polymerization, LBL self-assembly etc.These methods are all the Microencapsulation Method based on chemical reaction, have structure-controllable, performance is adjustable, be easy to give the features such as various feature functionalitys.But more loaded down with trivial details, the consuming time length of these method operating process, and be easy to introduce impurity etc., which has limited their practical application.
The method that microcapsule is prepared in ultrasonic radiation is invented in the early 1990s by people such as the scientist Suslick of the U.S., and they utilize the ultrasonic radiation of high strength gas or water-fast liquid protein cross cyst membrane bag to be carried, and are prepared into protein microcapsules.This method is simple to operate, and efficient quick is environmental protection again, and the microcapsule obtained has good biocompatibility, has potential using value in fields such as drug techniques.
Graphene quantum dot is the nano material of accurate zero dimension, and the motion of its internal electron in all directions is all limited to, so quantum confinement effect is remarkable especially, has the character of many uniquenesses.In bio-imaging, theoretical and experimentally all confirm, quantum limitation effect and side effect can induce graphene quantum dot to send fluorescence.In field of biomedical research, conventional fluorescent labeling demarcates object of study, but can fluorescence be made to lose efficacy because of long firing time, and this makes the application of general fluorescent agent in biomedicine be restricted.Graphene quantum dot has stable fluorescence light source, not easily occurs that optical attenuation loses its fluorescence, and has good biocompatibility and aqueous stability, and being conducive to chemistry functional simultaneously and modifying, is a kind of good drug carrier material.Platinum, copper are combined with graphene quantum dot and prepare the carrier that inorganic hybridization capsule is used as fuel-cell catalyst by the people such as Wang by the method by template and double reduction and assembling.But yet there are no the report utilizing ultrasonic method to prepare cyst wall to contain the microcapsule of graphene quantum dot.
Summary of the invention:
Object of the present invention is just for above-mentioned the deficiencies in the prior art, provides the microcapsule with fluorescent tracing and reduction response medicine release function that a kind of cyst wall contains graphene quantum dot;
Another object of the present invention there is provided a kind of preparation method with fluorescent tracing and reduction response medicine release function microcapsule
The object of the invention is to be achieved through the following technical solutions:
One has fluorescent tracing and reduction response medicine release function microcapsule, by carrying out ultrasonic radiation to the aqueous solution of the sulfhydrylation graphene quantum dot obtained after simultaneously containing sulfydryl and amino organic polymer or biopolymer modification with the oil phase being mounted with hydrophobic drug, formed with the cross linking membrane containing graphene quantum dot as cyst wall, to be loaded with the drug-loading microcapsule of oil phase for core of hydrophobic drug, the size of microcapsule is between 0.2-20 μm.
Containing sulfydryl and amino organic polymer or biopolymer while described is contain sulfydryl and amino organic polymer or biopolymer while synthetic or biological extraction obtain as amino-Polyethylene Glycol-sulfydryl, amino-polyglutamic acid-sulfydryl, bovine serum albumin, fibroin albumen or soybean protein.
Described hydrophobic drug is one or more in paclitaxel, lomustine, ginsenoside, camptothecine or silymarin medicine.
Described oil phase be biological medicine can various animal oil, vegetable oil, microbial grease, mineral oil, silicone oil or the immiscible nontoxic liquid state organics of other and water.
There is a preparation method for fluorescent tracing and reduction response medicine release function microcapsule, comprise the following steps:
A, by the graphene oxide quantum dot ultrasonic disperse of 0.1-100mg in 5-200ml water;
B, at N
2under protection, inject and while 1-20ml concentration is 10mg/ml, to contain the mixed solution that sulfydryl and amino organic polymer or biopolymer and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml;
Stirring reaction 1-24 hour under c, oxygen free condition, 3-5 centrifuge washing, removes unreacted simultaneously containing sulfydryl and amino organic polymer or biopolymer, obtains sulfhydrylation graphene quantum dot;
D, by deionized water dispersed for the sulfhydrylation graphene quantum dot of preparation, with test tube measure according to a certain volume sulfhydrylation graphene quantum dot aqueous solution and containing the oil phase of hydrophobic drug, be placed in ice-water bath;
E, ultrasonic probe is placed in oil/water two-phase interface, under certain ultrasonic power, acts on a period of time;
F, centrifuge washing is carried out to reactant liquor, obtain with the cross linking membrane of sulfhydrylation graphene quantum dot for cyst wall, the drug-loading microcapsule being core with the oil phase of hydrophobic drug.
The volume ratio of the described aqueous solution containing sulfhydrylation graphene quantum dot and the oil phase containing hydrophobic drug is 1:1-20:1.
Described ultrasonic power is 100-1000W/cm
2, ultrasonic time is 1-20min.
Beneficial effect: graphene quantum dot can be evenly distributed on the cyst wall of microcapsule by the inventive method, by organic polymer or biopolymer to the modification of graphene quantum dot, increase the distance between graphene quantum dot, gathering between reducing to greatest extent because of graphene quantum dot and the generation of the fluorescent quenching caused, thus fluorescent tracing performance when ensureing its medicine transmission; The cyst wall containing graphene quantum dot formed plays bag to medicine and carries and protective effect; The reduction response of disulfide bond is utilized effectively to control the release of medicine; Preparation method of the present invention is fast and convenient, high-efficiency environment friendly, and material source is extensive, and products pure is nontoxic.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described in further detail:
Cyst wall contains the preparation method with the microcapsule of fluorescent tracing and reduction response medicine release function of graphene quantum dot, that the oil phase being mounted with hydrophobic drug is the drug-loading microcapsule of core with the cross linking membrane of the sulfhydrylation graphene quantum dot obtained after simultaneously simultaneously containing sulfydryl and amino organic polymer or biopolymer modification for cyst wall.
The sulfhydrylation graphene quantum dot obtained after simultaneously containing sulfydryl and amino organic polymer or biopolymer modification is distributed in aqueous phase; Hydrophobic drug is dispersed in oil phase, is made into the dispersion liquid immiscible with water; Then carry out ultrasonic radiation at the two-phase interface of oil/water, the microcapsule with fluorescent tracing and reduction response medicine release function that cyst wall contains graphene quantum dot can be obtained.
Containing sulfydryl and amino organic polymer or biopolymer can be contain sulfydryl and amino organic polymer or biopolymer while synthetic or biological extraction obtain as amino-Polyethylene Glycol-sulfydryl, amino-polyglutamic acid-sulfydryl, bovine serum albumin, fibroin albumen or soybean protein simultaneously.
Hydrophobic drug is one or more in paclitaxel, lomustine, ginsenoside, camptothecine or silymarin.
Described oil phase be biological medicine can various animal oil, vegetable oil, microbial grease, mineral oil, silicone oil or the immiscible nontoxic liquid state organics of other and water.
There is the preparation method of fluorescent tracing and reduction response medicine release function microcapsule, comprise the following steps:
A, by the graphene oxide quantum dot ultrasonic disperse of 0.1-100mg in 5-200ml water;
B, at N
2under protection, inject and while 1-20ml concentration is 10mg/ml, to contain the mixed solution that sulfydryl and amino organic polymer or biopolymer and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml;
Stirring reaction 1-24 hour under c, oxygen free condition, 3-5 centrifuge washing, removes unreacted simultaneously containing sulfydryl and amino organic polymer or biopolymer, obtains sulfhydrylation graphene quantum dot;
D, by deionized water dispersed for the sulfhydrylation graphene quantum dot of preparation, with test tube measure according to a certain volume sulfhydrylation graphene quantum dot aqueous solution and containing the oil phase of hydrophobic drug, be placed in ice-water bath;
E, ultrasonic probe is placed in oil/water two-phase interface, under certain ultrasonic power, acts on a period of time;
F, centrifuge washing is carried out to reactant liquor, obtain with the cross linking membrane of sulfhydrylation graphene quantum dot for cyst wall, the drug-loading microcapsule being core with the oil phase of hydrophobic drug.
The volume ratio of the described aqueous solution containing sulfhydrylation graphene quantum dot and the oil phase containing hydrophobic drug is 1:1-20:1.
Described ultrasonic power is 100-1000W/cm
2, ultrasonic time is 1-20min.
Principle: utilize high-strength ultrasonic in the catalytic action at oil/water interface, the sulfydryl on sulfhydrylation graphene quantum dot surface is impelled to occur crosslinked, form stable cross linking membrane, and by coated for the oil phase being loaded with hydrophobic drug, formed with the cross linking membrane containing graphene quantum dot for cyst wall, the oil phase being mounted with hydrophobic drug is the drug-loading microcapsule of core.
Embodiment 1
By the graphene oxide quantum dot ultrasonic disperse of 2mg in 20ml water; At N
2under protection, inject the mixed solution that amino-Polyethylene Glycol-sulfydryl that 5ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 12 hours under oxygen free condition, 3 centrifuge washings, remove unreacted amino-Polyethylene Glycol-sulfydryl, obtain sulfhydrylation graphene quantum dot; 1mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg lomustine is dissolved in 10ml methyl-silicone oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the methyl-silicone oil of lomustine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 400W power, ultrasonic irradiation 5min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 2
By the graphene oxide quantum dot ultrasonic disperse of 2mg in 20ml water; At N
2under protection, inject the mixed solution that amino-polyglutamic acid-sulfydryl that 5ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 16 hours under oxygen free condition, 4 centrifuge washings, remove unreacted amino-polyglutamic acid-sulfydryl, obtain sulfhydrylation graphene quantum dot; 1mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg paclitaxel is dissolved in 10ml hydroxy silicon oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the hydroxy silicon oil of paclitaxel, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 500W power, ultrasonic irradiation 8min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 3
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that bovine serum albumin that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 2 hours under oxygen free condition, 5 centrifuge washings, remove unreacted bovine serum albumin, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg ginsenoside is dissolved in 10ml soybean oil; With test tube by volume 6:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the soybean oil of ginsenoside, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 300W power, ultrasonic irradiation 8min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 4
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that bovine serum albumin that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 3 centrifuge washings, remove unreacted bovine serum albumin, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg ginsenoside is dissolved in 10ml soybean oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the soybean oil of ginsenoside, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 600W power, ultrasonic irradiation 5min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 5
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that fibroin albumen that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 5 centrifuge washings, remove unreacted fibroin albumen, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg camptothecine is dissolved in 10ml Oleum Arachidis hypogaeae semen; With test tube by volume 3:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the Oleum Arachidis hypogaeae semen of camptothecine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 700W power, ultrasonic irradiation 3min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 6
By the graphene oxide quantum dot ultrasonic disperse of 10mg in 100ml water; At N
2under protection, inject the mixed solution that soybean protein that 20ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 3 centrifuge washings, remove unreacted soybean protein, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg silymarin is dissolved in 10ml algal oil; With test tube by volume 8:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the algal oil of silymarin, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 500W power, ultrasonic irradiation 6min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 7
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that amino-Polyethylene Glycol-sulfydryl that 5ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 12 hours under oxygen free condition, 3 centrifuge washings, remove unreacted amino-Polyethylene Glycol-sulfydryl, obtain sulfhydrylation graphene quantum dot; 1mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg lomustine is dissolved in 10ml hydroxy silicon oil; With test tube by volume 4:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the hydroxy silicon oil of lomustine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 600W power, ultrasonic irradiation 5min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 8
By the graphene oxide quantum dot ultrasonic disperse of 2mg in 20ml water; At N
2under protection, inject the mixed solution that amino-polyglutamic acid-sulfydryl that 5ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 16 hours under oxygen free condition, 3 centrifuge washings, remove unreacted amino-polyglutamic acid-sulfydryl, obtain sulfhydrylation graphene quantum dot; 1mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg camptothecine is dissolved in 10ml hydroxy silicon oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the hydroxy silicon oil of camptothecine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 300W power, ultrasonic irradiation 8min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 9
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that bovine serum albumin that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 3 centrifuge washings, remove unreacted bovine serum albumin, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg lomustine is dissolved in 10ml methyl-silicone oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the methyl-silicone oil of lomustine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 600W power, ultrasonic irradiation 5min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 10
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that bovine serum albumin that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 3 centrifuge washings, remove unreacted bovine serum albumin, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg lomustine is dissolved in 10ml methyl-silicone oil; With test tube by volume 5:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the methyl-silicone oil of lomustine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 100W power, ultrasonic irradiation 20min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 11
By the graphene oxide quantum dot ultrasonic disperse of 5mg in 50ml water; At N
2under protection, inject the mixed solution that bovine serum albumin that 10ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 24 hours under oxygen free condition, 3 centrifuge washings, remove unreacted bovine serum albumin, obtain sulfhydrylation graphene quantum dot; 2mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg lomustine is dissolved in 10ml methyl-silicone oil; With test tube by volume 1:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the methyl-silicone oil of lomustine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 1000W power, ultrasonic irradiation 1min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Embodiment 12
By the graphene oxide quantum dot ultrasonic disperse of 2mg in 20ml water; At N
2under protection, inject the mixed solution that amino-polyglutamic acid-sulfydryl that 5ml concentration is 10mg/ml and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml; Stirring reaction 16 hours under oxygen free condition, 3 centrifuge washings, remove unreacted amino-polyglutamic acid-sulfydryl, obtain sulfhydrylation graphene quantum dot; 0.1mg sulfhydrylation graphene quantum dot is dispersed in 10ml deionized water, 10mg camptothecine is dissolved in 10ml hydroxy silicon oil; With test tube by volume 20:1 measure sulfhydrylation graphene quantum dot aqueous solution and containing the hydroxy silicon oil of camptothecine, be placed in ice-water bath; Ultrasonic probe is placed in oil/water two-phase interface, under 300W power, ultrasonic irradiation 8min; Centrifuge washing is carried out to reactant liquor, obtains the microcapsule that cyst wall contains graphene quantum dot.
Claims (7)
1. one kind has fluorescent tracing and reduction response medicine release function microcapsule, it is characterized in that, by carrying out ultrasonic radiation to the aqueous solution of the sulfhydrylation graphene quantum dot obtained after simultaneously containing sulfydryl and amino organic polymer or biopolymer modification with the oil phase being mounted with hydrophobic drug, formed with the cross linking membrane containing graphene quantum dot as cyst wall, to be loaded with the drug-loading microcapsule of oil phase for core of hydrophobic drug, the size of microcapsule is between 0.2-20 μm.
2. according to there is fluorescent tracing and reduce response medicine release function microcapsule described in right 1, it is characterized in that, containing sulfydryl and amino organic polymer or biopolymer while described is contain sulfydryl and amino organic polymer or biopolymer while synthetic or biological extraction obtain as amino-Polyethylene Glycol-sulfydryl, amino-polyglutamic acid-sulfydryl, bovine serum albumin, fibroin albumen or soybean protein.
3., according to having fluorescent tracing and reducing response medicine release function microcapsule described in right 1, it is characterized in that, described hydrophobic drug is one or more in paclitaxel, lomustine, ginsenoside, camptothecine or silymarin medicine.
4. according to there is fluorescent tracing and reduce response medicine release function microcapsule described in right 1, it is characterized in that, described oil phase be biological medicine can various animal oil, vegetable oil, microbial grease, mineral oil, silicone oil or the immiscible nontoxic liquid state organics of other and water.
5. there is a preparation method for fluorescent tracing and reduction response medicine release function microcapsule, it is characterized in that, comprise the following steps:
A, by the graphene oxide quantum dot ultrasonic disperse of 0.1-100mg in 5-200ml water;
B, at N
2under protection, inject and while 1-20ml concentration is 10mg/ml, to contain the mixed solution that sulfydryl and amino organic polymer or biopolymer and concentration are 1-(3-the dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride of 10mg/ml;
Stirring reaction 1-24 hour under c, oxygen free condition, 3-5 centrifuge washing, removes unreacted simultaneously containing sulfydryl and amino organic polymer or biopolymer, obtains sulfhydrylation graphene quantum dot;
D, by deionized water dispersed for the sulfhydrylation graphene quantum dot of preparation, with test tube measure according to a certain volume sulfhydrylation graphene quantum dot aqueous solution and containing the oil phase of hydrophobic drug, be placed in ice-water bath;
E, ultrasonic probe is placed in oil/water two-phase interface, under certain ultrasonic power, acts on a period of time;
F, centrifuge washing is carried out to reactant liquor, obtain with the cross linking membrane of sulfhydrylation graphene quantum dot for cyst wall, the drug-loading microcapsule being core with the oil phase of hydrophobic drug.
6. according to the preparation method with fluorescent tracing and reduction response medicine release function microcapsule according to claim 5, it is characterized in that, the volume ratio of the described aqueous solution containing sulfhydrylation graphene quantum dot and the oil phase containing hydrophobic drug is 1:1-20:1.
7., according to the preparation method with fluorescent tracing and reduction response medicine release function microcapsule according to claim 5, it is characterized in that, described ultrasonic power is 100-1000W/cm
2, ultrasonic time is 1-20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510736204.6A CN105267991A (en) | 2015-11-03 | 2015-11-03 | Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510736204.6A CN105267991A (en) | 2015-11-03 | 2015-11-03 | Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105267991A true CN105267991A (en) | 2016-01-27 |
Family
ID=55138376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510736204.6A Pending CN105267991A (en) | 2015-11-03 | 2015-11-03 | Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105267991A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106596678A (en) * | 2017-02-16 | 2017-04-26 | 济南大学 | Preparation method of electrochemiluminescent biosensor based on polydopamine nano-microsphere loaded graphene quantum dot |
| CN106978146A (en) * | 2017-04-06 | 2017-07-25 | 上海电力学院 | The microcapsule phase-change particle and preparation method of composite Nano copper and graphene quantum dot |
| CN106984247A (en) * | 2017-05-11 | 2017-07-28 | 青岛大学 | A kind of preparation method for the polymer microcapsule for carrying hydrophilic material |
| CN107019681A (en) * | 2017-03-24 | 2017-08-08 | 武汉轻工大学 | A kind of shell load medicine protein microcapsules and preparation method containing ZnO quantum dot and magnetic particle simultaneously |
| CN107500272A (en) * | 2017-09-21 | 2017-12-22 | 深圳市贝特瑞新能源材料股份有限公司 | The composite that oil/water two-phase interface prepares the method for graphene/quantum dot nano composite and obtained |
| CN109078195A (en) * | 2018-08-30 | 2018-12-25 | 青岛大学 | The thermosensitive type of shell containing graphene quantum dot, kernel containing magnetic nano-particle carries micro- organogel of medicine and the preparation method and application thereof |
| CN109476841A (en) * | 2016-07-30 | 2019-03-15 | 日本化药株式会社 | Novel high polymer derivative and the novel high polymer derivative image probe for using it |
-
2015
- 2015-11-03 CN CN201510736204.6A patent/CN105267991A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| FRANCESCA CAVALIERI等: ""One-pot ultrasonic synthesis of multifunctional microbubbles and microcapsules using synthetic thiolated macromolecules"", 《CHEMCOMM》 * |
| XUEJUN CUI等: ""A Facile Sonochemical Route for the Fabrication of Magnetic Protein Microcapsules for Targeted Delivery"", 《CHEM. EUR. J》 * |
| ZHANFENG LI等: ""Synthesis of multifunctional bovine serum albumin microcapsules by the sonochemical method for targeted drug delivery and controlled drug release"", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109476841B (en) * | 2016-07-30 | 2021-08-24 | 日本化药株式会社 | Novel polymer derivative and novel polymer derivative imaging probe using same |
| US10869937B2 (en) | 2016-07-30 | 2020-12-22 | Nippon Kayaku Kabushiki Kaisha | Polymer derivatives, and novel polymer derivative imaging probe using same |
| CN109476841A (en) * | 2016-07-30 | 2019-03-15 | 日本化药株式会社 | Novel high polymer derivative and the novel high polymer derivative image probe for using it |
| CN106596678A (en) * | 2017-02-16 | 2017-04-26 | 济南大学 | Preparation method of electrochemiluminescent biosensor based on polydopamine nano-microsphere loaded graphene quantum dot |
| CN106596678B (en) * | 2017-02-16 | 2017-11-07 | 济南大学 | A kind of preparation method for the electrochemical luminescence biology sensor that graphene quantum dot is loaded based on poly-dopamine nanoparticle |
| CN107019681A (en) * | 2017-03-24 | 2017-08-08 | 武汉轻工大学 | A kind of shell load medicine protein microcapsules and preparation method containing ZnO quantum dot and magnetic particle simultaneously |
| CN106978146A (en) * | 2017-04-06 | 2017-07-25 | 上海电力学院 | The microcapsule phase-change particle and preparation method of composite Nano copper and graphene quantum dot |
| CN106984247A (en) * | 2017-05-11 | 2017-07-28 | 青岛大学 | A kind of preparation method for the polymer microcapsule for carrying hydrophilic material |
| CN106984247B (en) * | 2017-05-11 | 2019-11-12 | 青岛大学 | A kind of preparation method for the polymer microcapsule carrying hydrophilic material |
| CN107500272B (en) * | 2017-09-21 | 2020-10-16 | 贝特瑞新材料集团股份有限公司 | Method for preparing graphene/quantum dot nanocomposite through oil/water two-phase interface and obtained composite |
| CN107500272A (en) * | 2017-09-21 | 2017-12-22 | 深圳市贝特瑞新能源材料股份有限公司 | The composite that oil/water two-phase interface prepares the method for graphene/quantum dot nano composite and obtained |
| CN109078195A (en) * | 2018-08-30 | 2018-12-25 | 青岛大学 | The thermosensitive type of shell containing graphene quantum dot, kernel containing magnetic nano-particle carries micro- organogel of medicine and the preparation method and application thereof |
| CN109078195B (en) * | 2018-08-30 | 2021-04-02 | 青岛大学 | Thermosensitive drug-loaded micro-organic gel with shell layer containing graphene quantum dots and core containing magnetic nanoparticles, and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105267991A (en) | Microcapsule with fluorescent tracing and reduction responsive drug release functions and its preparation method | |
| Cai et al. | Designing bioactive micro-/nanomotors for engineered regeneration | |
| CN105310998A (en) | Microcapsule containing functionalized graphene in capsule wall and preparation method of microcapsule | |
| Mallepally et al. | Hydrogen peroxide filled poly (methyl methacrylate) microcapsules: potential oxygen delivery materials | |
| Song et al. | All-aqueous electrosprayed emulsion for templated fabrication of cytocompatible microcapsules | |
| Chang et al. | Enzyme encapsulated hollow silica nanospheres for intracellular biocatalysis | |
| Zhang et al. | Core-shell microparticles: From rational engineering to diverse applications | |
| US6562361B2 (en) | Pheromone immobilized in stable hydrogel microbeads | |
| CN100425375C (en) | Nano-class silver colloidal solution and preparation method thereof | |
| CN105085754A (en) | Microcapsule with poly butanediol dimethylacrylate as wall material and preparing method of microcapsule | |
| He et al. | Near-infrared photo-controlled permeability of a biomimetic polymersome with sustained drug release and efficient tumor therapy | |
| CN101053814A (en) | Multiple responsive high molecular microcapsule and preparation method thereof | |
| CN105080445A (en) | Method for preparing microcapsule with tannic acid-ferric ion polymer serving as wall material by taking micro emulsion as template by interface reaction | |
| CN100558408C (en) | The porous calcium carbonate microsphere is calcium alginate gel beads of shell and preparation method thereof | |
| Zhang et al. | Research progress of advanced microneedle drug delivery system and its application in biomedicine | |
| Wu et al. | Dual-porosity hollow carbon spheres with tunable through-holes for multi-guest delivery | |
| Khanmohammadi et al. | Cell encapsulation in core-shell microcapsules through coaxial electrospinning system and horseradish peroxidase-catalyzed crosslinking | |
| CN103735531A (en) | Preparation method for microcapsules with magnetic nanoparticles-containing capsule walls | |
| Huang et al. | Fabrication of multicore milli-and microcapsules for controlling hydrophobic drugs release using a facile approach | |
| CN104418971A (en) | Glucose oxidase mediation free radical initiating system and method for preparing hydrogel by using glucose oxidase mediation free radical initiating system | |
| Qu et al. | Aqueous two-phase droplet-templated colloidosomes composed of self-formed particles via spatial confined biomineralization | |
| CN104893825A (en) | Fragrance microcapsule with polyurea as capsule wall and preparation method of fragrance microcapsule | |
| Li et al. | Liquid metal swimming nanorobots | |
| US9878039B1 (en) | Microcapsule having a microcapsule shell material that is rupturable via a retro-dimerization reaction | |
| CN106311275A (en) | Preparation method of magnetic core-shell type Fe3O4@SiO2-Ag nanoparticles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160127 |
|
| WD01 | Invention patent application deemed withdrawn after publication |