CN102879399A - Red blood cell and solid wall surface high-speed collision microscopic visual experiment device - Google Patents
Red blood cell and solid wall surface high-speed collision microscopic visual experiment device Download PDFInfo
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- CN102879399A CN102879399A CN2012103610448A CN201210361044A CN102879399A CN 102879399 A CN102879399 A CN 102879399A CN 2012103610448 A CN2012103610448 A CN 2012103610448A CN 201210361044 A CN201210361044 A CN 201210361044A CN 102879399 A CN102879399 A CN 102879399A
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Abstract
The invention relates to a red blood cell and solid wall surface high-speed collision microscopic visual experiment device, which comprises a high-speed camera, an optical microscope, an injector, a micro-injection pump, an image acquisition computer, a micro channel, a waste liquid collection cup and a light source, wherein the micro channel is fixed on an objective table; an inlet hole and an outlet hole are respectively connected with the injector and the waste liquid collection cup through pipelines; the injector is arranged on the micro-injection pump; the optical microscopic is arranged above the micro channel; the high-speed camera is arranged above the optical microscope and is connected with the image acquisition computer; the light source is arranged below the micro channel; red blood cell suspension is loaded in the injector; and during experiment, the flow speed of the red blood cell suspension in the micro channel is 0.1 to 3 m/s. The red blood cell and solid wall surface high-speed collision microscopic visual experiment device can perform microscopic visual research on hemolysis injury under a high-speed collision condition during moving of red blood cells, so that a morphologic change rule of the red blood cells can be obtained; and a theoretical basis and a reference basis are supplied to hemolysis research under the high-speed collision condition.
Description
[technical field]
The present invention relates to a kind of red blood cell-solid wall surface high velocity impact photomicrography experimental provision.
[background technology]
Cardiac failure serious threat patient life.Drug therapy and therapeutic method of surgery all are difficult to obtain obvious effect, and the organ transplant treatment is faced with the very few difficulty of donor.In order to obtain better result for the treatment of, medical worker and researchist's expectation utilize the artificial mechanism blood pump to keep the interior blood circulation of patient body, save patient's life, the quality of making the life better.Yet, because the blade rotational speed in the blood pump turns up to several thousand, even up to ten thousand turning, cause the red blood cell in the blood under high-speed motion, to bump with parts such as blood pump blade, walls, and then cause haemolysis, and namely erythrocyte membrane breaks, and haemoglobin occurs free, red blood cell can't work, and this also becomes the bottleneck problem of artificial blood pump development.Tradition research thinks that the interior erythrocyte hemolysis of blood pump is because the shearing force that produces that flows causes, and rarely has research for the haemolysis that red blood cell in the blood motion and blood pump parts high velocity impact cause, and erythrocytic flowing velocity is lower in the at present correlative study, so study erythrocytic haemolysis problem under the high velocity impact condition, have very important engineering and academic significance comprehensively.
[summary of the invention]
The object of the present invention is to provide a kind of red blood cell-solid wall surface high velocity impact photomicrography experimental provision, realize the photomicrography of red blood cell-solid wall surface high velocity impact, erythrocytic motion process and the process that bumps with solid wall surface under observation different in flow rate and the different collision wall condition, and obtain instantaneous picture in a large amount of motions and the collision process, the haemolysis under the high velocity impact condition is studied.
For achieving the above object, the present invention is achieved by the following technical solutions.
A kind of red blood cell-solid wall surface high velocity impact photomicrography experimental provision comprises high speed camera, optical microscope, syringe, micro-injection pump, image acquisition computer, microchannel, waste collection cup and two light source; The microchannel is fixed on the objective table, and the ingate links to each other with the waste collection cup with syringe respectively by pipeline with outlet opening; Syringe is installed on the micro-injection pump; Optical microscope is arranged at the top, microchannel, and high speed camera is arranged at the optical microscope top and links to each other with image acquisition computer; Two light sources are arranged at the below of microchannel.
The present invention further improves and is: load red blood cell suspension in the described syringe, during experiment the microchannel in the flowing velocity of red blood cell suspension be 0.1m/s-3m/s, flow velocity is higher, is higher than the flow velocity in present other correlative studys.
The present invention further improves and is: the microchannel is selected and blood and red blood cell biocompatibility material preferably.
The present invention further improves and is: the material of microchannel is dimethyl silicone polymer.
The present invention further improves and is: described pair of light source is two light sources that micro-light source and fluorescence light source consist of; Be provided with optical filter in the optical microscope.
The present invention further improves and is: the microchannel is T or Y shape, and the microchannel comprises an ingate and two outlet openings.
The present invention further improves and is: optical microscope amplifies cell movement and collision process and observe, and high speed camera is used for obtaining the transient images of collision process, and carries out image and export in image acquisition computer.
The present invention further improves and is: in preparation red blood cell suspension process, use micron order internal diameter screen pack that red blood cell suspension is carried out multiple times of filtration.
The present invention further improves and is: in the experiment, at set intervals red blood cell suspension in the syringe is carried out homogenising.
With respect to prior art, the present invention has the following advantages:
The present invention has set up a kind of experimental system for simulating to red blood cell in the blood pump and solid wall surface high velocity impact process, realize the photomicrography of red blood cell-solid wall surface high velocity impact, erythrocytic motion process and the process that bumps with solid wall surface under observation different in flow rate and the different collision wall condition, and obtain instantaneous picture in a large amount of motions and the collision process, the haemolysis under the high velocity impact condition is studied; For illustrating Hemolytic mechanism, set up the haemolysis model, determine to affect the correlative study such as parameter and provide fundamental basis and reference data.
Among the present invention, under the promotion of power system, red blood cell is flow through in the experiment microchannel, and microchannel solid wall surface generation high velocity impact; Utilize the high speed micro imaging system to erythrocytic motion and collision process is observed and imaging; In the experiment in the microchannel measurement flowing velocity scope of red blood cell suspension be 0.1m/s-3m/s, flowing velocity is higher, is higher than the flow velocity (mm/s or cm/s magnitude) at present other correlative study.Because erythrocytic size is about 5-10 μ m, and erythrocytic movement velocity is higher, erythrocytic flow channel selects to be of a size of micron-sized microchannel.When selecting the making material of microchannel, fully take into account blood and erythrocytic biocompatibility.The microchannel is divided into two kinds of structures in T shape microchannel and Y shape microchannel (T shape and Y shape microchannel are representing respectively impingement area relatively flat and relative bending in the blood pump), and two kinds of each self-contained multiple different sizes of different structure.Red blood cell suspension under the finite concentration made from new blood centrifugal filtration passes through the microchannel as the experiment fluent solution.As power system, syringe contain to carry an experiment red blood cell suspension with the micro-injection pump pushing syringe, utilizes the permanent or pulsating flow of micro-injection pump to promote to realize experiment under the certain flow rate.By changing the flow of micro-injection pump, realize the experiment under the different in flow rate., utilize optical microscope that cell movement and collision process are amplified and observe as imaging system with optical microscope and high speed camera and computing machine, utilize the transient images in the high speed camera acquisition process, and in computing machine, carry out image output.In addition, in order to make the imaging region in the high-speed motion more obvious, in optical microscope, installed optical filter, utilize simultaneously the double light path of micro-light path and fluorescence light path combination that the visual field is illuminated, effectively increase field luminance, improve observation and the image effect that obtains.
The present invention can observe red blood cell flow at high speed and collision process in the microchannel, and obtain flowing and the Collision diagram picture under the different in flow rate, can further obtain the Variation Regularity of Morphological Characteristics of red blood cell in motion process, for the research of the haemolysis under the high velocity impact condition provides necessary foundation.
[description of drawings]
Fig. 1 is the experimental system schematic diagram;
Fig. 2 is T shape Micro Channel Architecture overall diagram;
Fig. 3 is T shape microchannel test section partial enlarged drawing;
Fig. 4 is Y shape Micro Channel Architecture overall diagram;
Fig. 5 is Y shape microchannel test section partial enlarged drawing.
[embodiment]
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail.
See also Fig. 1 to shown in Figure 5, at first microchannel 7 is described.Microchannel 7 structures are shown in Fig. 2,3,4,5, and wherein T shape and Y shape microchannel represent respectively relatively flat and crooked collision wall.Microchannel 7 is of a size of micron order, can make as required the microchannel of different size.When selecting the making material of microchannel, fully take into account blood and erythrocytic biocompatibility, can selection and blood and red blood cell biocompatibility material preferably, be preferably PDMS (polydimethylsiloxane, dimethyl silicone polymer).The measurement flowing velocity scope of microchannel 7 interior red blood cell suspensions is 0.1m/s-3m/s in the experiment, and flowing velocity is higher, is higher than the flow velocity at present other correlative studys.
See also shown in Figure 1, a kind of red blood cell of the present invention-solid wall surface high velocity impact photomicrography experimental provision, comprise high speed camera 1, optical microscope 2, syringe 3, micro-injection pump 4, image acquisition computer 5, microchannel 7, waste collection cup 8 and two light source 9,10.Microchannel 7 is fixed on the objective table, contains an ingate 71 and two outlet openings 72, and ingate 71 links to each other with waste collection cup 8 with syringe 3 respectively by pipeline 6 with outlet opening 72.During work, red blood cell suspension 71 flows into from the ingate under dynamic action, through 7 inside, microchannel, and then flows out to waste collection cup 8 from outlet opening 72.
With reference to Fig. 1, the experimental provision power system comprises micro-injection pump 4 and syringe 3.Syringe 3 installs on micro-injection pump 4, and links to each other with the ingate, microchannel by pipeline.The red blood cell suspension for preparing is carried in the syringe 3, under the dynamic action of micro-injection pump 4, in the microchannel, forms the permanent or fluctuating flow of red blood cell suspension.In the process of preparation red blood cell suspension, use screen pack that red blood cell suspension is carried out multiple times of filtration, prevent that effectively impurity is to the interference of experiment.At set intervals, need to carry out homogenising to the red blood cell suspension in the syringe 3, with the impact that prevents that concentration stratification from producing experiment.Can realize the experiment under the different in flow rate by changing the flow set of micro-injection pump 4, obtain the result and compare.
With reference to Fig. 1, the experimental provision imaging system comprises optical microscope 2, high speed camera 1 and image acquisition computer 5.Optical microscope 2 is arranged at 7 tops, microchannel, utilizes the mobile collision process that reaches of 2 pairs of microchannels of optical microscope, 7 interior red blood cells to amplify and directly observation.High speed camera 1 links to each other with image acquisition computer 5, high speed camera 1 is arranged at optical microscope 2 tops, look like to obtain with Collision diagram by flowing after 2 amplifications of 1 pair of process of high speed camera optical microscope, and utilize image acquisition computer 5 to carry out image output, under a kind of flow velocity operating mode, can obtain multiple series of images.Be provided with optical filter in the optical microscope 2, the double light path that adopts micro-light source 9 and fluorescence light source 10 to combine illuminates the visual field, thereby effectively increases field luminance, improves picture quality.Thereby realize the photomicrography of red blood cell-solid wall surface high velocity impact experiment.
During experimental provision work, open micro-injection pump 4, flow is set, pushing syringe 3, red blood cell suspension be 7 interior formation Steady Flows in the microchannel.Utilize optical microscope 2 to observe, high speed camera 1 carries out imaging, image acquisition computer 5 image data and output image.
By experiment, observe to erythrocytic motion process with the high velocity impact process of solid wall surface, obtain erythrocytic motion and Changing Pattern, realized the photomicrography of red blood cell-solid wall surface high velocity impact, the haemolysis under the high velocity impact condition is studied; By changing flow velocity, the red blood cell movement velocity is studied the impact of haemolysis; By the experiment of different structure microchannel, collision wall structure feature is studied the impact of haemolysis, for the research of the haemolysis under the high velocity impact condition is provided fundamental basis and reference frame.
Claims (9)
1. red blood cell-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: comprise high speed camera (1), optical microscope (2), syringe (3), micro-injection pump (4), image acquisition computer (5), microchannel (7), waste collection cup (8) and two light source (9,10); Microchannel (7) is fixed on the objective table, and ingate (71) link to each other with waste collection cup (8) with syringe (3) respectively by pipeline with outlet opening (72); Syringe (3) is installed on the micro-injection pump (4); Optical microscope (2) is arranged at top, microchannel (7), and high speed camera (1) is arranged at optical microscope (2) top and links to each other with image acquisition computer (5); Two light sources are arranged at the below of microchannel (7).
2. a kind of red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: load red blood cell suspension in the described syringe, the flowing velocity of the interior red blood cell suspension in microchannel during experiment (7) is 0.1m/s-3m/s.
3. red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: microchannel (7) select the material that has biocompatibility with blood and red blood cell.
4. red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: the material of microchannel (7) is dimethyl silicone polymer.
5. red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision is characterized in that: described pair of light source is two light sources that micro-light source (9) and fluorescence light source (10) consist of; Optical microscope is provided with optical filter in (2).
6. red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: microchannel (7) are T or Y shape, and microchannel (7) comprise an ingate (71) and two outlet openings (72).
7. red blood cell according to claim 1-solid wall surface high velocity impact photomicrography experimental provision, it is characterized in that: optical microscope (2) amplifies cell movement and collision process and observes, high speed camera (1) is used for obtaining the transient images of high velocity impact process, and carries out image output in image acquisition computer (5).
8. red blood cell according to claim 2-solid wall surface high velocity impact photomicrography experimental provision is characterized in that: in preparation red blood cell suspension process, use micron order internal diameter screen pack that red blood cell suspension is carried out multiple times of filtration.
9. red blood cell according to claim 2-solid wall surface high velocity impact photomicrography experimental provision is characterized in that: in the experiment, at set intervals red blood cell suspension in the syringe is carried out homogenising.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398371A (en) * | 2018-02-09 | 2018-08-14 | 浙江大学 | A kind of reproducting method of the analysis and Rheologic of haemocyte shear stress rheology observation device and haemocyte shear stress Rheological Regularity |
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| WO2006104899A2 (en) * | 2005-03-25 | 2006-10-05 | Massachusetts Institute Of Technology | System and method for hilbert phase imaging |
| CN101216601A (en) * | 2007-12-29 | 2008-07-09 | 中国科学院西安光学精密机械研究所 | Method and device for accomplishing dark-field photomicrography and fluorescent photomicrography by axicon lens |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006104899A2 (en) * | 2005-03-25 | 2006-10-05 | Massachusetts Institute Of Technology | System and method for hilbert phase imaging |
| CN101216601A (en) * | 2007-12-29 | 2008-07-09 | 中国科学院西安光学精密机械研究所 | Method and device for accomplishing dark-field photomicrography and fluorescent photomicrography by axicon lens |
Non-Patent Citations (1)
| Title |
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| TAKANOBU YAGI，ETAL: "《COLLISION DYNAMICS OF RED BLOOD CELLS USING HIGH-SPEED IMPINGING MICROJETS》", 《10TH INTERNATIONAL CONFERENCE ON FLUID CONTROL，MEASUREMENTS，AND VISUALIZATION》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398371A (en) * | 2018-02-09 | 2018-08-14 | 浙江大学 | A kind of reproducting method of the analysis and Rheologic of haemocyte shear stress rheology observation device and haemocyte shear stress Rheological Regularity |
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Application publication date: 20130116 |