CN104323043A - Preparation method of feed additive with functions of improving weight gain and immunity of broiler chickens - Google Patents
Preparation method of feed additive with functions of improving weight gain and immunity of broiler chickens Download PDFInfo
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- CN104323043A CN104323043A CN201410667293.9A CN201410667293A CN104323043A CN 104323043 A CN104323043 A CN 104323043A CN 201410667293 A CN201410667293 A CN 201410667293A CN 104323043 A CN104323043 A CN 104323043A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to the fields of poultry animal nutrition and feed, and in particular relates to a preparation method of a feed additive with functions of improving weight gain and immunity of broiler chickens. The method comprises the following steps: taking 10-15 parts by weight of pine needles and 15-30 parts by weight of echinacea, drying at the temperature of 65 DEG C until the weight is constant, crushing, and screening by a 40-mesh sieve; and taking 30-40 parts by weight of bacillus subtilis, 15-25 parts by weight of enterococcus faecium and 10-15 parts by weight of carrier, evenly mixing the bacillus subtilis, the enterococcus faecium and the carrier with the pine needle powder and the echinacea powder to obtain the feed additive. The feed additive is simple in components, environment-friendly, free from toxic and side effects, less in consumption, low in price, remarkable in effect and simple in production and use methods; the actual feeding test proves that the feed additive is capable of remarkably improving the daily weight ratio of the broiler chickens, reducing the feed conversion ratio, remarkably increasing the number of T-lymphocytes of the chickens and the feed conversion rate, improving the growth performance of the broiler chickens and the immunologic function of the broiler chickens, is free from residue in the bodies of the broiler chickens and does not influence the meat quality of the chickens.
Description
Technical field
The present invention relates to poultry field of feed additive technology, specifically a kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization.
Background technology
Along with the raising of people's living standard, increasing to the consumption demand of meat product, chicken is nourished as the tradition in a kind of old hundred confidence the basic meat material of food materials and fast service restaurant industry, and supply-demand relationship is more nervous, has driven developing rapidly of broiler breeding industry thus.Enterprise is in order to enhance productivity, reduce production cost, all kinds of feed addictive is added in broiler fodder of being everlasting, but the feed addictive sold on the market is at present mostly containing antibiotic and hormone medicine, to accelerate growth of meat chicken speed and to prevent disease from occurring, these antibiotic and hormone not only can destroy the ecological balance in chicken body and natural environment between microorganism, and can have residual in chicken, by food chain, the disease treatment having a strong impact on people is even hereditary, and harm humans is healthy, in addition, at present containing antibiotic feed addictive complicated component, also can have a huge impact the meat of chicken, usually the bad smell that one is also difficult to by culinary art cover is had in chicken, have a strong impact on the appetite of people, but completely need not any chicken containing antibiotic feed addictive, under current rearing conditions, not only poor growth, and easily there is disease, cause production cost high, the problems such as profit margin is low, therefore, research and development one both can improve broiler growth speed and immunity, do not affect chicken meat again, and the Broiler chicks feed be improved environment is very necessary.
Summary of the invention
Object of the present invention is exactly the feed addictive for generally adding in current feeding of broiler process containing antibiotic and hormone medicine, destroy ecological environment, the problem of harm humans health, provides a kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization.Feed addictive of the present invention has promotion broiler growth, increases livestock and poultry resistance against diseases, reduces the advantages such as feed cost.
A kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization of the present invention, be made up of following step: get pine needle 10 ~ 15 weight portion, Echinacea 15 ~ 30 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 30 ~ 40 weight portion, VREF 15 ~ 25 weight portion, carrier 10 ~ 15 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.
The preferred a kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization of the present invention, be made up of following step: get pine needle 12 weight portion, Echinacea 22 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 34 weight portion, VREF 22 weight portion, carrier 10 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.
The viable bacteria content of described bacillus subtilis is 1,000 hundred million cfu/g, and the viable bacteria content of VREF is 10,000,000,000 cfu/g.
Described carrier be corn flour or or maize cob meal or calcium carbonate in any one.
Broiler chicks feed addictive of the present invention in use, adds in Broiler chicks basal diet by the weight percentage proportioning of 0.02% ~ 0.06%.
The pine needle meal used in the present invention, containing crude protein 8.96%, crude fat 11.1%, crude fibre 27.1%, NFE 41.5%, ash content 3.43%, vitamin A 950 ~ 2203 mg/kg, also containing abundant vitamin C, vitamin E and selenium, adds 3% ~ 5% pine needle meal in daily ration of broiler, daily gain can improve 8.1% ~ 12.0%, and feed return rate improves 8.4%.Meanwhile, containing phytocide and the necessary vitamin of growth of animal in pine needle meal, there is effect of disease resistance, effectively can resist the generation of broiler chicken disease.
The Echinacea used in the present invention has enhancing immunologic function and antiinflammatory action, and helps body fight bacterium and virus.Modern pharmacological research proves that Echinacea can stimulating immune system cellular activity, and when these cells have activity, white blood cell more promptly causing the invader of disease to surround, make virus or bacterial death, preventing them from breeding, or stopping their activity.
The bacillus subtilis used in the present invention, the free oxygen in environment can be consumed rapidly, cause enteron aisle hypoxemia, promote that useful anaerobic bacteria grows, obvious inhibitory action is had to the conditioned pathogen of pathogenic bacteria or autogenous infection, and produce amylase, protease etc., promote digesting and assimilating of nutriment, reduce trophism diarrhea; Can also reduce toxic and harmful in ight soil, as the generation of ammonia, indoles material, improves in house and natural environment.
The VREF used in the present invention, it is the one in lactic acid bacteria, this bacterium is a kind of facultative anaerobe, the beneficial bacterium of multiple enzyme, vitamin (mainly B family vitamin) can be produced, a large amount of lactic acid and multiple somatomedin can be produced in animal body, as additive for farm animal feed, the diarrhea that cub fowl causes because of intestinal bacilli illness can be prevented and treated, there is regulating intestinal canal flora imbalance and cut off pathogen infection approach, suppress the effect of harmful bacteria growth, and can improve food conversion ratio, reduce production cost.VREF metabolism can produce the material such as organic acid, hydrogen peroxide bacteriocin, these materials have suppression pathogen and spoilage organisms, improve immunity, improve the physiological functions such as livestock products quality, the bacteriocin that wherein metabolism produces can effectively treat intractable diseases common in plant, as having antagonism to staphylococcus, clostridium, salmonella and Shigella, and it is residual not leave medicine; A large amount of lactic acid that metabolism produces, can significantly reduce animal intestinal pH, keep the sour environment of enteron aisle, suppress the growth of pathogen, peroxidating Hydrogen Energy suppresses and kills Gram-negative bacteria and hydrogen peroxide positive bacteria as pseudobactin, coliform bacteria, Salmonella.
Well-chosen of the present invention two kinds of Chinese herbal medicines and two kinds of natural microbial bacterium are matched the feed addictive made, composition is simple, environmental protection, have no side effect, addition is few, cheap, Be very effective, produce and using method simple, find through actual feeding experiment, feed addictive of the present invention can significantly improve the day anharmonic ratio reduce feed-weight ratio of Broiler chicks, remarkable increase chicken T lymphocyte quantity and improve food conversion ratio, the growth performance of Broiler chicks can be improved, strengthen the immunologic function of Broiler chicks, and without any residual in Broiler Chicken, to chicken meat without any harmful effect.
Detailed description of the invention
Embodiment 1
By optimization formula of the present invention, to produce 1000kg feed addictive of the present invention, concrete production process is as follows:
Get pine needle 120kg, Echinacea 220kg dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 340kg, VREF 220kg, corn flour 100kg mixes with pine needle meal, Echinacea powder, to obtain final product.The viable bacteria content of described bacillus subtilis is 1,000 hundred million cfu/g, and the viable bacteria content of VREF is 10,000,000,000 cfu/g.
The feed addictive produced by the present embodiment adds in Broiler chicks feed by the weight percentage proportioning of 0.02% ~ 0.06%.
Effect of feed addictive obtained by checking the present embodiment, in Hubei, animal experiment does in Wei Aibi agriculture and animal husbandry Science and Technology Ltd., and content of the test is as follows:
1. experimental animal and experimental design
Choose the healthy AA Broiler chicks of 300 1 ages in days, be divided into 5 groups at random, often organize 6 repetitions, each repetition 10 chickens.1 group is control group; 2,3 and 4 groups is test group, and 5 groups is aureomycin group, and 2 groups are added feed addictive (1T complete feed adds 200g feed addictive) prepared by the present embodiment by the weight percentage proportioning of 0.02%, are low dose group in basal diet; 3 groups are added feed addictive (1T complete feed adds 400g feed addictive) prepared by the present embodiment by the weight percentage proportioning of 0.04%, are middle dosage group in basal diet; 4 groups are added feed addictive (1T complete feed adds 600g feed addictive) prepared by the present embodiment by the weight percentage proportioning of 0.06%, are high dose group in basal diet; 5 groups is aureomycin 50ppm.The feed addictive of research variable concentrations is to the research of the nutrition aspects such as Broiler chicks daily gain, feed-weight ratio, and the impact of the chicken immune index such as bursal index, index and spleen index, T cent lymphocytes, thymus index, leukocytes phagocytic india ink.Test daily ration adopts Corn-soybean-fish meal type basal diet, basis is (1,2,3,4 group are not added any antibiotic) such as corn, wheat bran, fish meal, dregs of beans, trace element and vitamins, all test daily rations are powder, are prepared by Hubei Wei Aibi agriculture and animal husbandry Science and Technology Ltd..Basal diet trophic level is in table 1.
Table 1: test chicken basal diet dietary nutritional standard %
| Composition | Crude protein | Crude fibre | Coarse ash | Calcium | Total phosphorus | Manganese | Moisture |
| Content | 20.0-20.9 | ≤3.6 | ≤6 | 0.8-1.0 | 0.61-0.7 | 0.30-0.80 | 12.7 |
Test chicken is supported in ground, and level land is raised, free choice feeding and freely drink water, the infrared insulation of brood time, and house interior humiture and illumination control etc. all routinely feeding and management requirement are carried out.From 1 week age, age in formal test to 28 week terminated, 28 weeks by a definite date.Experimental design is in table 2.
Table 2 experimental design scheme
| Group | Process |
| 1 group | Basal diet |
| 2 groups | Basal diet+0.02% feed addictive |
| 3 groups | Basal diet+0.04% feed addictive |
| 4 groups | Basal diet+0.06% feed addictive |
| 5 groups | Basal diet+50ppm aureomycin |
2. the collection of blood sample
During respectively at 21 ages in days and 42 age in days, each in each group of test to repeat to choose body weight substantially identical and close to each 2 plumages of bouncing broiler chicken of average quality, claim gross weight ramming material, at the epilation of triangle place, shirtfront, cotton ball soaked in alcohol sterilization, Culling heart blood, take blood sample.For avoiding polluting, strict disposable blood taking needle and the vacuum tube of using is taken a blood sample, and every plumage blood-sample withdrawal 2 is managed, and does following arrangement respectively: a pipe 2ml does anti-freezing process, measures Blood Physiological Indexes; Another blood sampling tube sample 5ml, not anti-freezing process, blood is placed under room temperature, solidify, 3000rpm centrifugal after get serum 0.5ml, make the mensuration of Biochemical Indices In Serum.
3. Testing index and method
(1) growth performance
Start to carry out empty stomach to chicken in 0,7,14,21,28,35 and 42d 7:00 in morning respectively to weigh, and add up all feed consumption rates, calculate all gains in weight and feed-weight ratio, calculate average daily gain amount (ADG), average daily ingestion amount (ADFI) and feed-weight ratio (F/G) etc., the results are shown in following table 3.
(2) mensuration of immune indexes
T TLC adopts Suan – naphthyl acetate esterase (ANAE) decoration method to measure; T lymphocyte transformation test is with colorimetric method (tetramethyl tetrazolium bromide).Thymus gland, spleen, the bursal index percentage of this immune organ weight percentage of liveweight (not having the body weight of fat) represents its relative weight (g/100g body weight).Leukocytic phagocytic function: add appropriate india ink in heparin anti-coagulating, leave standstill 10min at 37 DEG C of temperature, smear-dyeing-microscopy, count 200 leucocytes, calculate the leucocyte percentage engulfing ink particles, measurement result sees the following form 4.
4. testing result and analysis
(1) various dose feed addictive is on the impact of broiler growth performance
Table 3 broiler growth performance table
Note: colleague's data shoulder note
arepresentative significant difference compared with control group,
aarepresentative differs extremely remarkable with control group; Colleague 2,3, between 4 and 5 data shoulder note
brepresent significant difference
bbrepresent that difference is extremely remarkable.
The daily gain of low dosage additive group and feed-weight ratio significant difference compared with control group can be found out from the data of upper table 3, in extremely remarkable with feed-weight ratio difference compared with control group with the daily gain of high dose additive group, and neutralize and compare daily gain between high additive group and feed-weight ratio difference is not remarkable.
(2) various dose feed addictive is on the impact of Broiler chicks Immune Organs Index.
Table 4 Broiler chicks Immune Organs Index
Note: colleague's data shoulder note
arepresentative significant difference compared with control group,
aarepresentative differs extremely remarkable with control group; Data shoulder between 2,3,4 and 5 groups of going together is noted
brepresent significant difference
bbrepresent that difference is extremely remarkable
From data in upper table 4, compared with 1 group, interpolation feed addictive and antibiotic group can significantly increase index and spleen index, bursal index and thymus index, significant difference.Compared with 1 group, high dose feed addictive Immune Organs Index all significantly increases, and feed addictive group Immune Organs Index slightly increases compared with antibiotic group, but difference is not remarkable.
(3) various dose feed addictive is along with the increase in (day) age in week is on the impact of Broiler chicks T cent lymphocytes.
Along with the increase in (day) age in week, all there is the trend increased in each group T cent lymphocytes.During 4 week age, feed addictive group T cent lymphocytes is significantly higher than control group and antibiotic group 5 (P<0.05); During 5 week age, except 2 groups of T cent lymphocytes are a little less than except blank group, other each group is all significantly higher than blank group (P<0.05), wherein the cent lymphocytes of feed addictive 4 groups is the highest, compared with other each group, difference extremely significantly (P<0.01); During 6 week age, feed addictive group and antibiotic group T cent lymphocytes are all significantly higher than control group (P<0.05), and when 7 week age, feed addictive group and antibiotic group T cent lymphocytes were still significantly higher than control group.
(4) various dose feed addictive is on the impact of Broiler chicks leukocytes phagocytic rate.
When 4-6 age in week, each group leukocytes phagocytic rate all presents increases trend gradually, and feed addictive association has dosage to increase trend, falls after rise to some extent during 7 week age.When 4 week age, compared with blank group, feed addictive group and antibiotic group leukocytes phagocytic rate higher, significant difference (P<0.05), along with the increase of feed addictive group concentration, leukocytes phagocytic rate also increases gradually, and compared with antibiotic group, feed addictive group leukocytes phagocytic rate pole significantly improves; During 5 week age, feed addictive 2 groups of leukocytes phagocytic rates are a little less than control group, and other each group is all significantly higher than control group; During 6 week age, feed addictive respectively organizes leukocytes phagocytic rate all higher than control group, significant difference.During 7 week age, each group leukocytes phagocytic rate all decreases, but feed addictive group leukocytes phagocytic rate is still significantly higher than control group and antibiotic group.
Through the above results and analysis, can find out use feed addictive of the present invention to the growth performance of Broiler chicks, Immune Organs Index increases, T cent lymphocytes increases, the increase of leukocytes phagocytic rate all has obvious effect, its result of the test is significantly better than control group, also have compared with antibiotic group and significantly increase or slightly increase, prove that feed addictive of the present invention has the effect improving Broiler chicks weightening finish and immunization really.In above-mentioned three dosage groups, every test data of 3 groups (using basal diet+0.04% feed addictive) and the test data of antibiotic group are more or less the same, and can be used as the optimal dosage replacing antibiotic to add basal diet.
Separately get the Broiler chicks of each group of 42 more than age in days 1000g, fragrant with tasting its meat after the method culinary art of braising in soy sauce, stewing in clear soup respectively, find that feed addictive group chick fine and tender taste perfume is sliding, without any peculiar smell, and the thick bavin of antibiotic group chick meat, mouthfeel difference also with fishy smell, illustrates feed addictive of the present invention to Broiler chicks meat without any harmful effect.
Embodiment 2
Get pine needle 10 weight portion, Echinacea 30 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 40 weight portion, VREF 15 weight portion, carrier 10 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.The viable bacteria content of described bacillus subtilis is 1,000 hundred million cfu/g, and the viable bacteria content of VREF is 10,000,000,000 cfu/g.
Embodiment 3
Get pine needle 15 weight portion, Echinacea 15 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 30 weight portion, VREF 25 weight portion, carrier 15 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.The viable bacteria content of described bacillus subtilis is 1,000 hundred million cfu/g, and the viable bacteria content of VREF is 10,000,000,000 cfu/g.
Claims (4)
1. one kind is improved the preparation method of the feed addictive of Broiler chicks weightening finish and immunization, it is characterized in that being made up of following step: get pine needle 10 ~ 15 weight portion, Echinacea 15 ~ 30 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 30 ~ 40 weight portion, VREF 15 ~ 25 weight portion, carrier 10 ~ 15 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.
2. a kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization according to claim 1, it is characterized in that being made up of following step: get pine needle 12 weight portion, Echinacea 22 weight portion dries to constant weight through 65 DEG C, pulverize, cross 40 mesh sieves, get bacillus subtilis 34 weight portion, VREF 22 weight portion, carrier 10 weight portion mix with pine needle meal, Echinacea powder, to obtain final product.
3. a kind of preparation method improving the feed addictive of Broiler chicks weightening finish and immunization according to claim 1 and 2, is characterized in that: the viable bacteria content of described bacillus subtilis is 1,000 hundred million cfu/g, and the viable bacteria content of VREF is 10,000,000,000 cfu/g.
4. a kind of preparation method of feed addictive improving Broiler chicks weightening finish and immunization according to claim 1 and 2, is characterized in that: described carrier be corn flour or or maize cob meal or calcium carbonate in any one.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982659A (en) * | 2015-06-15 | 2015-10-21 | 广东大华农动物保健品股份有限公司 | Composition containing natural plant raw materials and preparation method and application thereof |
| CN109043136A (en) * | 2018-07-20 | 2018-12-21 | 隆昌康诺生物科技有限公司 | A kind of compound Echinacea extraction pulvis improving animal disease resistant power |
| CN113397059A (en) * | 2021-06-16 | 2021-09-17 | 华兴宠物食品有限公司 | Probiotic herbal puppy food and preparation method thereof |
| CN115119900A (en) * | 2022-06-29 | 2022-09-30 | 中国农业科学院北京畜牧兽医研究所 | Compound probiotic composition and application thereof as feed additive in pigeon breeding |
-
2014
- 2014-11-20 CN CN201410667293.9A patent/CN104323043A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982659A (en) * | 2015-06-15 | 2015-10-21 | 广东大华农动物保健品股份有限公司 | Composition containing natural plant raw materials and preparation method and application thereof |
| CN109043136A (en) * | 2018-07-20 | 2018-12-21 | 隆昌康诺生物科技有限公司 | A kind of compound Echinacea extraction pulvis improving animal disease resistant power |
| CN113397059A (en) * | 2021-06-16 | 2021-09-17 | 华兴宠物食品有限公司 | Probiotic herbal puppy food and preparation method thereof |
| CN115119900A (en) * | 2022-06-29 | 2022-09-30 | 中国农业科学院北京畜牧兽医研究所 | Compound probiotic composition and application thereof as feed additive in pigeon breeding |
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