CN104285892B - A kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain - Google Patents
A kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain Download PDFInfo
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- CN104285892B CN104285892B CN201410420985.3A CN201410420985A CN104285892B CN 104285892 B CN104285892 B CN 104285892B CN 201410420985 A CN201410420985 A CN 201410420985A CN 104285892 B CN104285892 B CN 104285892B
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- nicotine
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- SNICXCGAKADSCV-JTQLQIEISA-N Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 169
- 229960002715 Nicotine Drugs 0.000 title claims abstract description 169
- 229930015196 nicotine Natural products 0.000 title claims abstract description 169
- 230000001488 breeding Effects 0.000 title claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 117
- 239000000203 mixture Substances 0.000 claims abstract description 98
- 238000009472 formulation Methods 0.000 claims abstract description 90
- 239000007864 aqueous solution Substances 0.000 claims abstract description 51
- 230000001058 adult Effects 0.000 claims abstract description 33
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 28
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 27
- 239000004467 fishmeal Substances 0.000 claims abstract description 27
- 230000032683 aging Effects 0.000 claims abstract description 26
- 241000382353 Pupa Species 0.000 claims abstract description 6
- 239000002361 compost Substances 0.000 claims description 48
- 241000257193 Sarcophaga peregrina Species 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 15
- 235000008939 whole milk Nutrition 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 11
- 235000019504 cigarettes Nutrition 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000005728 strengthening Methods 0.000 claims description 7
- FFRBMBIXVSCUFS-UHFFFAOYSA-N Martius yellow Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic Effects 0.000 claims description 5
- 230000003252 repetitive Effects 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 241000282898 Sus scrofa Species 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000011068 load Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 10
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 238000002372 labelling Methods 0.000 abstract description 2
- 230000001418 larval Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 31
- 241000208125 Nicotiana Species 0.000 description 16
- 240000008962 Nicotiana tabacum Species 0.000 description 15
- 238000000034 method Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 241000257159 Musca domestica Species 0.000 description 8
- 230000000051 modifying Effects 0.000 description 8
- 230000000749 insecticidal Effects 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 210000000538 Tail Anatomy 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 235000013360 fish flour Nutrition 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 210000003491 Skin Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- 210000003608 Feces Anatomy 0.000 description 2
- 210000004080 Milk Anatomy 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N Potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 229960000539 carbamide Drugs 0.000 description 2
- 235000013877 carbamide Nutrition 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000000474 nursing Effects 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- DVARTQFDIMZBAA-UHFFFAOYSA-O Ammonium nitrate Chemical compound [NH4+].[O-][N+]([O-])=O DVARTQFDIMZBAA-UHFFFAOYSA-O 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
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- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 208000008425 Protein Deficiency Diseases 0.000 description 1
- 101700050571 SUOX Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000003466 anti-cipated Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000004059 degradation Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003000 nontoxic Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000607 poisoning Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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Abstract
The invention discloses the breeding method of a kind of resistance to nicotine Sarcophga fuscicauda strain.Described breeding method is to be seeded in nicotine-containing first formulation material by the newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind, by newly hatched larvae cultivate aging to larva, pupate, fly pupa utilizes the second formulation material to feed adult after sprouting wings, after it produces newly hatched larvae, cultivate through 20～30 continuous domestication from generation to generation, stable resistance to nicotine Sarcophga fuscicauda strain can be obtained.Described first formulation material is made up of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, being adjusted to humidity with the nicotine aqueous solution of mass percent concentration 3 ‰ is 60%.The present invention directly uses nicotine aqueous solution and wheat bran and the imported fish meal scientific compatibility of suitable concentration, the first formulation material obtained the most successfully covers the unpleasant of nicotine and bitterness, nutritional labeling is suitable, it is ensured that Sarcophga fuscicauda larval growth is smooth, and guarantees obtaining stable cultivation effect.
Description
Technical field
The present invention relates to insecticide Cultivating techniques field, be specifically related to the cultivation side of a kind of resistance to nicotine Sarcophga fuscicauda strain
Method.
Background technology
Inapplicable fresh tobacco leaf refers to not to be suitable for the fresh tobacco leaves (water content 80～90%) of cured tobacco production, big with document
The waste/hypo-tobacco leaf produced in the production process of flue-cured tobacco that amount is mentioned or discarded tobacco leaf (water content about 15%) are different.Inapplicable Nicotiana tabacum L. leads to
Referring to after cigarette strain normally binds and remove 2～3 milk foot leaves, cigarette industry is inapplicable, need to be under field removing processes
Portion's leaf and upper leaf, mainly include bottom cigarette strain because of illumination deficiency, dysplasia, inclusions is not enough, anticipated roasting after inferior quality,
Do not possess 2 inferior leads that baking is worth, and cigarette strain top hack bad, curtailment, structure are stiff, roasting after inferior quality
2 tops.
If these inapplicable Nicotiana tabacum Lves arbitrarily abandon field, that easily causes environmental pollution and tobacco virus repeats sense
Dye, the processing mode destroyed or fill of concentrating according to prior art then needs to spend substantial amounts of expense, and current industry is per ton not
Being suitable for the processing cost averagely up to 200 yuan of Nicotiana tabacum L., each Nicotiana tabacum L. producing region generally requires under the organization leadership of government of local party committee,
Carry out policy propaganda, tabulation registration, build treatment facility, unified remove, record archive, publicity, cash the measures such as subsidy and supervise
Carry out the process of inapplicable Nicotiana tabacum L., waste time and energy costly.
It is a kind of preferably alternate process method, ring that the insecticide using resistance to nicotine carries out biological treatment to inapplicable Nicotiana tabacum L.
Protect, high financial profit.In order to utilize insecticide to realize inapplicable fresh tobacco leaf of carrying out a biological disposal upon, first need to filter out the insecticide product of resistance to nicotine
Kind.
The applicant studies for a long period of time based on to housefly and inapplicable Nicotiana tabacum L. treatment technology, sums up and utilizes inapplicable Nicotiana tabacum L.
Housefly is carried out screening, strengthening and the breeding method of resistance to nicotine strain.Described cultivation is to be connect by the newly hatched larvae of resistance to nicotine housefly
Planting in the first formulation material, fly pupa feeds adult with the second formulation material after sprouting wings, and the continuous domestication through 20～30 generations obtains resistance to cigarette
Alkali housefly kind.The composition that it is critical only that the first formulation material and the second formulation material of its method is to ensure the smooth of resistance to nicotine housefly
Growth.First formulation material consist of inapplicable fresh tobacco leaf 75～80%, wheat bran 10～15%, imported fish meal 5～10%；Second joins
Square stock is whole milk powder 70%, brown sugar 29.8～29.9%, pure nicotine 0.1～0.2%.
This technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but different batches cultivates the aging of acquisition
The quantity difference of fly larvae is relatively big, and resistance to nicotine ability is the most otherwise varied, there is the uncertainty of effect.Main reason is that inapplicable
The application aspect of Nicotiana tabacum L., although sum up the discomfort adding zone of reasonableness in formulation material through creative research and great many of experiments
The control to Musca domestica larva toxic action can be ensured with Nicotiana tabacum L., but the factor affecting not nicotine content of tobacco leaves is the most various, mechanism
Complicated, it is more difficult to realize accurately controlling.The factor affecting nicotine content in tobacco leaf specifically includes that (1) different growing stages, institute in Nicotiana tabacum L.
Different containing nicotine content: in tobacco seed, nicotine content is 0, seedling raising process rises beginning and is formed at root, after along with root system
Developing, synthesis and the accumulation ability of nicotine gradually strengthen, so different growing stages, in Nicotiana tabacum L., contained nicotine content is different；
(2) cigarette strain different parts, nicotine content is dramatically different, and nicotine content is also by leaf position and number of sheets large effect；(3) different cultivars
Nicotine amount contained by Nicotiana tabacum L. is different；(4) weather conditions etc. of soil dry-wet all have the impact of different savings to nicotine content；(5) agriculture
The different appreciable impact nicotine content of tobacco leaves of skill measure, including fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and cut
Nicotine content in root fertilizer, picking methods etc. factor all appreciable impact Nicotiana tabacum Lves, as a example by fertilization type, the nitrogen of three kinds of forms
In fertilizer, after carbamide is used, nicotine accumulation is the highest, and ammonium nitrate takes second place, and the nicotine accumulation that potassium nitrate processes is minimum, lower than using carbamide
27%；As a example by Physical property, Zhang Dan etc. studies and finds, postpones nicotine accumulation intensity peak with Physical property and declines successively, nicotine
Content lowers the most successively, often postpones and pinching for one day, and nicotine content just declines 0.0504%；Again as a example by cutting root fertilizer, cut root 1/8
Compared with not cutting root, upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13 percentage points,
The range of decrease 51.4%.How avoiding the uncertainty using different batches compost to cultivate the aging fly larvae effect obtained is this area
Technical barrier urgently to be resolved hurrily.And employing is stablized feasible technical scheme and is cultivated the insect strain of resistance to nicotine of more multi items and have
Effect ground continuous domestication stabilizes it, and is this area simultaneously need to the important technological problems that solves.
Summary of the invention
The technical problem to be solved in the present invention is to lack lacking of resistance to nicotine Sarcophga fuscicauda kind in prior art to overcome
Fall into, it is provided that the breeding method of a kind of resistance to nicotine Sarcophga fuscicauda strain, the inventive method is not by inapplicable Nicotiana tabacum L. various factors shadow
Ring, stable cultivation effect can be obtained.
The purpose of the present invention is achieved by the following technical programs:
Sarcophga fuscicauda is different from a kind of insecticide of housefly, and it is a kind of important economic insects and scavenger insect, can
Sarcophga fuscicauda is utilized to process various corrupt organic waste, such as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc..Existing
Sarcophga fuscicauda kind all has preferable biological treatment capabilities to nontoxic organic waste, but the process to inapplicable fresh tobacco leaf is imitated
Fruit is the best.Main cause is because in inapplicable fresh tobacco leaf containing toxicants such as a certain amount of nicotine, to Sarcophga fuscicauda children
Worm (maggots of boettcherisca peregrine) has the effect of certain murder by poisoning and Developing restraint.In order to utilize Sarcophga fuscicauda biological treatment discomfort
With fresh tobacco leaf, first need to filter out the Sarcophga fuscicauda kind of resistance to nicotine from wild Sarcophga fuscicauda kind, improve palm fibre tail the numbest
The fly tolerance to fresh tobacco leaf.
The present invention provides the breeding method of a kind of resistance to nicotine Sarcophga fuscicauda strain, comprises the steps:
S1. the Sarcophga fuscicauda of the nicotine aqueous formulation material that mass percent concentration is 3 ‰ will be tolerated under indoor conditions
The newly hatched larvae of kind is seeded in the first formulation material, by Sarcophga fuscicauda newly hatched larvae cultivate aging to larva, pupate；
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it produces newly hatched larvae, enters next generation
Acclimatization culture；
S3. by above-mentioned steps repetitive operation, cultivate through 20～30 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine palm fibre
The other sarcophagid strain of tail；
Wherein, described first formulation material is made up of each component of following percetage by weight: wheat bran 70～90%, imported fish meal
10～30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%；
Second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7～29.8% and
Nicotine 0.2～0.3%.
Preferably, described first formulation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal
20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
Preferably, described second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar
29.7% and nicotine 0.3%.
Described nicotine can use the commercial products of purity >=99.5%.
The compound method of described first formulation material is by after each component mixing of the first formulation material, adds extra fine quality percentage
The humidity of formulation material is adjusted to 60% by the nicotine aqueous solution of specific concentration.
Sarcophga fuscicauda newly hatched larvae 0.5～1.0 g is accessed among described every 1 kg the first formulation material.The most described every 1
Kg the first formulation material accesses Sarcophga fuscicauda newly hatched larvae 0.8 g.
Described tolerance mass percent concentration is that the resistance to nicotine Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ is
Refer to from wild Sarcophga fuscicauda kind, first filter out resistance to nicotine Sarcophga fuscicauda kind, the resistance to cigarette that will screen the most again
Alkali Sarcophga fuscicauda kind carries out hot housing so that it is tolerance mass percent concentration is the nicotine aqueous formulation material of 3 ‰.
Described from wild Sarcophga fuscicauda kind, filter out resistance to nicotine Sarcophga fuscicauda kind method particularly includes:
S01. configuration lures collection compost, and collection compost will be lured to load in open containers；
The described collection compost each component that contains following percetage by weight that lures: wheat bran 70～90%, imported fish meal 10～30%,
With the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 60%；
S02., when weather is fine, the morning, 8:00～9:00 will be equipped with luring the container of collection compost to be put in house refuse respectively
Heap, chicken house and pig farm lure the wild Sarcophga fuscicauda newly hatched larvae of collection, and afternoon, 17:00～18:00 regained the container being put in everywhere；
S03. each container reclaimed is placed on indoor cultivation, waits the wild Sarcophga fuscicauda newly hatched larvae luring collection to arrive to cultivate extremely
When larva is aging, utilize being separated by fly larvae from food phase behavior of maggots of boettcherisca peregrine with formulation material；
S04. by the Morphological Identification of Sarcophga fuscicauda larva, maggots of boettcherisca peregrine is chosen；
S05. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda of resistance to nicotine
Kind.
Described the resistance to nicotine Sarcophga fuscicauda kind screened is carried out hot housing so that it is tolerance mass percent is dense
Degree is the nicotine aqueous formulation material of 3 ‰ method particularly includes:
S11. the starting stage: the newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described
Initial incubation material is made up of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, use percent mass
It is 60% that above-mentioned formulation material is adjusted to humidity by the nicotine aqueous solution of specific concentration 1 ‰；In room conditions, by above-mentioned Sarcophga fuscicauda
Newly hatched larvae cultivate aging to larva, pupate, sprout wings after, feed its adult；
S12. the 1st strain: after the adult of S11 produces newly hatched larvae, the newly hatched larvae produced uses the 1st time
Hot housing material carries out cultivating to be cultivated；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 70～
90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is
The value of 60%, S is selected from 0.1～0.5；In room conditions, by above-mentioned Sarcophga fuscicauda newly hatched larvae cultivate aging to larva,
After pupating, sprouting wings, feed its adult；
S13. the N+1 time strain: the newly hatched larvae of the adult product of n-th strain gained is strengthened again
Cultivate, and component contained by the compost used by the N+1 time strain meets following ratio: the percent mass of nicotine aqueous solution
Specific concentration=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats strengthening, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last breeding phase
Only.
Its adult of described nursing is all to utilize whole milk powder, the mixture of brown sugar and water to feed.Preferably, according to weight hundred
Mark meter, described whole milk powder, the usage ratio of brown sugar are whole milk powder 70%, brown sugar 30%；The mode that water is fed is to utilize sea
Silk floss is saturated with water, sucks for adult.
Preferably, the described indoor conditions cultivating Sarcophga fuscicauda is temperature 25～30 DEG C, humidity 30～70%.
The invention has the beneficial effects as follows:
The present invention the most directly uses nicotine aqueous solution and wheat bran and the imported fish meal scientific compatibility of suitable concentration, obtains
The first formulation material obtained the most successfully covers the unpleasant of nicotine and bitterness, and nutritional labeling is suitable, it is ensured that Sarcophga fuscicauda larva is raw
Long smoothly, and because the appropriateness of nicotine content and definitiveness, and the first formulation material and the second formulation material nutrient substance and
The reasonability arranged before and after nicotine content, it is ensured that in same nurturing an environment, the formula of the same weight composition of different batches
The maggots of boettcherisca peregrine rate that material obtains is essentially identical, it is thus achieved that stable cultivation effect.
The resistance to nicotine Sarcophga fuscicauda strain of acquisition is cultivated owing to have passed through the long-term domestication in 20～30 generations through the present invention
Cultivate, not only adapted to culture conditions, and defined more stable hereditary character, stablizing of its offspring resistance to nicotine ability
Property fine, and cultivate the resistance to nicotine Sarcophga fuscicauda strain of acquisition (only through screening and the palm fibre of hot housing without the present invention
The other sarcophagid of tail) toleration of nicotine easily degenerated by offspring.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.Unless stated otherwise, the present invention use reagent,
Equipment is the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, and purity is >=99.5%.
Embodiment 1 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost by each component of following percetage by weight
Composition: wheat bran 90%, imported fish meal 10%, is adjusted to humidity with the nicotine aqueous solution of mass percent concentration 1 ‰ by above-mentioned formulation material
It is 60%.
S02. by the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained everywhere
Plastic tub, takes back indoor cultivation.
The most in room conditions (temperature 25 DEG C, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae training that collection will be lured to arrive
Educate to larva aging, from food phase behavior, utilize maggots of boettcherisca peregrine separates fly larvae with formulation material that (i.e. maggots of boettcherisca peregrine is old
Can climb voluntarily time ripe from compost, find the place being dried and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, get rid of it
His fly kind.
S05. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can to obtain the brown tail of resistance to nicotine the numbest
Fly kind.
Embodiment 2 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost by each component of following percetage by weight
Composition: wheat bran 80%, imported fish meal 20%, is adjusted to wet with the nicotine aqueous solution of mass percent concentration 1.5 ‰ by above-mentioned formulation material
Degree is 60%.
S02. by the above-mentioned compost modulated, every 1kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained everywhere
Plastic tub, takes back indoor cultivation.
The most in room conditions (temperature 25 DEG C, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae training that collection will be lured to arrive
Educate to larva aging, from food phase behavior, utilize maggots of boettcherisca peregrine separates fly larvae with formulation material that (i.e. maggots of boettcherisca peregrine is old
Can climb voluntarily time ripe from compost, find the place being dried and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, get rid of it
His fly kind.
S05. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can to obtain the brown tail of resistance to nicotine the numbest
Fly kind.
Embodiment 3 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost by each component of following percetage by weight
Composition: wheat bran 70%, imported fish meal 30%, is adjusted to humidity with the nicotine aqueous solution of mass percent concentration 2 ‰ by above-mentioned formulation material
It is 60%.
S02. by the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained everywhere
Plastic tub, takes back indoor cultivation.
The most in room conditions (temperature 25 DEG C, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae training that collection will be lured to arrive
Educate to larva aging, from food phase behavior, utilize maggots of boettcherisca peregrine separates fly larvae with formulation material that (i.e. maggots of boettcherisca peregrine is old
Can climb voluntarily time ripe from compost, find the place being dried and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, get rid of it
His fly kind.
S5. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can to obtain the brown tail of resistance to nicotine the numbest
Fly kind.
Embodiment 4 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screened makes it tolerate percent mass
Specific concentration is the formulation material of the nicotine aqueous solution of 3 ‰
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1～3 any embodiment screened is cultivated so that it is tolerance
Mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 90%, enter
Mouth fish flour 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and it is the numbest that every basin accesses palm fibre tail
Fly newly hatched larvae 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), at the beginning of above-mentioned Sarcophga fuscicauda, children is incubated
Worm cultivate aging to larva, pupate, sprout wings after, feed its adult.Its adult of described nursing be utilize whole milk powder, brown sugar mixed
Compound and water are fed.Preferably, according to percent by weight, described whole milk powder, the usage ratio of brown sugar are whole milk powder
70%, brown sugar 30%；The mode that water is fed is to utilize sponge to be saturated with water, sucks for adult.Lower same.
Strain:
S12. the 1st strain: after the adult until the starting stage produces newly hatched larvae, the newly hatched larvae produced uses
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: bran
Skin 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is
60%, S are selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), incubate at the beginning of above-mentioned Sarcophga fuscicauda
Larvae cultivation is aging to larva, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain: the newly hatched larvae of the adult product of n-th strain gained is strengthened again
Cultivate, and component contained by the compost used by the N+1 time strain meets following ratio: the percent mass of nicotine aqueous solution
Specific concentration=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats strengthening, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.1‰、1.2‰、1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、
2.3‰、2.4‰、2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences altogether
20 hot housing.The Sarcophga fuscicauda kind that this stage turns out i.e. has higher tolerance to nicotine.
Embodiment 5 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screened makes it tolerate percent mass
Specific concentration is the formulation material of the nicotine aqueous solution of 3 ‰
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1～3 any embodiment screened is cultivated so that it is tolerance
Mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 80%, enter
Mouth fish flour 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and it is the numbest that every basin accesses palm fibre tail
Fly newly hatched larvae 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), at the beginning of above-mentioned Sarcophga fuscicauda, children is incubated
Worm cultivate aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the adult until the starting stage produces newly hatched larvae, the newly hatched larvae produced uses
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: bran
Skin 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is
60%, S are selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), incubate at the beginning of above-mentioned Sarcophga fuscicauda
Larvae cultivation is aging to larva, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain: the newly hatched larvae of the adult product of n-th strain gained is strengthened again
Cultivate, and component contained by the compost used by the N+1 time strain meets following ratio: the percent mass of nicotine aqueous solution
Specific concentration=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats strengthening, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.2‰、1.4‰、1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely at the beginning of Sarcophga fuscicauda
Incubate larva and experience 10 hot housing altogether.The Sarcophga fuscicauda kind that this stage turns out i.e. has higher tolerance to nicotine.
Embodiment 6 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screened makes it tolerate percent mass
Specific concentration is the formulation material of the nicotine aqueous solution of 3 ‰
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1～3 any embodiment screened is cultivated so that it is tolerance
Mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70%, enter
Mouth fish flour 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and it is the numbest that every basin accesses palm fibre tail
Fly newly hatched larvae 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), at the beginning of above-mentioned Sarcophga fuscicauda, children is incubated
Worm cultivate aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the adult until the starting stage produces newly hatched larvae, the newly hatched larvae produced uses
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: bran
Skin 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is
60%, S are selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), incubate at the beginning of above-mentioned Sarcophga fuscicauda
Larvae cultivation is aging to larva, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain: the newly hatched larvae of the adult product of n-th strain gained is strengthened again
Cultivate, and component contained by the compost used by the N+1 time strain meets following ratio: the percent mass of nicotine aqueous solution
Specific concentration=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats strengthening, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.5‰、2.0‰、2.5‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences 4 hot housing altogether.This stage turns out
Sarcophga fuscicauda kind i.e. nicotine is had higher tolerance.
More than 7 continuous domestication from generation to generation of embodiment are cultivated and are obtained stable resistance to nicotine Sarcophga fuscicauda strain
The nicotine aqueous solution that tolerance mass percent concentration is 3 ‰ embodiment 4～6 any embodiment cultivated is joined
The Sarcophga fuscicauda kind of square stock through the continuous domestication cultivation of multiple generations, forms stable resistance to nicotine Sarcophga fuscicauda product again
System.Method particularly includes:
S1., under indoor conditions, the Sarcophga fuscicauda of the nicotine aqueous formulation material that mass percent concentration is 3 ‰ will be tolerated
The newly hatched larvae of kind is seeded in the first formulation material, by Sarcophga fuscicauda newly hatched larvae cultivate aging to larva, pupate；
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it produces newly hatched larvae, enters next generation
Acclimatization culture；
S3. by above-mentioned steps repetitive operation, cultivate through 30 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine palm fibre tail is other
Sarcophagid strain；
The preparation of the first formulation material: described first formulation material is made up of each component of following percetage by weight: wheat bran 90%,
Imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
By the above-mentioned formulation material modulated, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm), every basin accesses Sarcophga fuscicauda newly hatched larvae 0.8 g；
In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated extremely
Larva is aging, pupate；
Fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and the second formulation material is by each component of following percetage by weight
Composition: whole milk powder 70.0%, brown sugar 29.8% and nicotine 0.2%, described percent is percetage by weight, treats that it produces and just incubates children
After worm, enter next acclimatization culture from generation to generation；
By above-mentioned steps repetitive operation, cultivate through 30 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine palm fibre tail is the numbest
Fly strain.
Embodiment 8
Other operations are with embodiment 7, and the most described first formulation material is made up of each component of following percetage by weight:
Wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
Described second formulation material is made up of each component of following percetage by weight: whole milk powder 70.00%, brown sugar 29.85% and nicotine
0.25%.Cultivate through 25 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine Sarcophga fuscicauda strain.
Embodiment 9
Other operations are with embodiment 7, and the most described first formulation material is made up of each component of following percetage by weight:
Wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
Described second formulation material is made up of each component of following percetage by weight: whole milk powder 70.00%, brown sugar 29.7% and nicotine
0.3%.Cultivate through 20 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine Sarcophga fuscicauda strain.
Comparative example 1
Operation with embodiment 1, except for the difference that described in lure collection compost be made up of each component of following percetage by weight: wheat bran
95%, imported fish meal 5%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Experiment
Find to utilize and as above lure collection compost almost to lure collection to come to produce newly hatched larvae less than Sarcophga fuscicauda kind.
Comparative example 2
Operation with embodiment 1, except for the difference that described in lure collection compost be made up of each component of following percetage by weight: wheat bran
80% and imported fish meal 20%.Experiment find to utilize as above lure collection compost lure collect to newly hatched larvae be not the most the product of resistance to nicotine
Kind.
Embodiment 10
The Sarcophga fuscicauda kind, the embodiment 4 to 6 that the embodiment of the present invention 1 to 3 are screened tolerate 3 ‰ through cultivating
The Sarcophga fuscicauda kind of nicotine formulation material, the embodiment 7 to the 9 Sarcophga fuscicauda kind through too much cultivating for continuous domestication,
Sarcophga fuscicauda kind after each comparative example processes carries out resistance to nicotine aptitude tests, and test process is:
Take the newly hatched larvae 0.5g that the Sarcophga fuscicauda adult after described embodiment cultivation processes is produced respectively, be respectively connected to
(wheat bran 80%, imported fish meal 20%, use percent mass to the formulation material containing better quality percent concentration nicotine aqueous solution of 1kg weight
It is 60% that above-mentioned formulation material is adjusted to humidity by the nicotine aqueous solution of specific concentration 3.0 ‰) in, test result is shown in Table 1:
Table 1
Test result illustrates, comparative example 1,2 Sarcophga fuscicauda kind is not resistant to the nicotine water of mass percent concentration 3.0 ‰
Solution.
Embodiment 1 to 3 is adjusted at the nicotine aqueous solution of mass percent concentration 3.0 ‰ through the Sarcophga fuscicauda kind of screening
There occurs diapause phenomenon in the formulation material of system, larval weight is relatively light, and has relatively large death.Embodiment 4 to 9 hot housing processes
After the resistance to nicotine ability of Sarcophga fuscicauda kind be greatly improved, the nicotine that can tolerate mass percent concentration 3.0 ‰ is water-soluble
The formulation material of liquid modulation.
Embodiment 11 stability experiment
The resistance to nicotine Sarcophga fuscicauda kind obtained through method described in embodiment 4 to 6 is carried out conventional cultivation (wherein fly larvae
Culture material formula is wheat bran 80%+ fish flour 20%, and with clear water, above-mentioned formulation material being adjusted to humidity is 60%, and adult forage formula is complete
Fat milk powder 70%+ brown sugar 30%) standby after 20 generations.Meanwhile, by the numbest for the resistance to nicotine palm fibre tail that obtains through method described in embodiment 7 to 9
Fly strain utilizes to cultivate expects that (wherein fly larvae culture material formula is wheat bran 80%, imported fish meal 20%, uses mass percent concentration
It is 60% that above-mentioned formulation material is adjusted to humidity by the nicotine aqueous solution of 3.0 ‰, and adult forage formula is whole milk powder 70.00%, brown sugar
29.7% and nicotine 0.3%) the most persistently cultivated for 20 generations after standby.The Sarcophga fuscicauda kind of above-mentioned two strain is carried out the energy of resistance to nicotine
Power is tested, and detection culture material formula is wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰
The humidity that above-mentioned formulation material is adjusted to formulation material is 60%, and whether the resistance to nicotine ability detecting its offspring declines.Test result is shown in Table
Shown in 2:
The stability experiment result of the table 2 Sarcophga fuscicauda strain after 30 generations
| Process | Larvae development go through the phase (my god) | Larva bar number/g(bar) | Survival rate of larvae (%) |
| The conventional strain of embodiment 4 | 7 | 21 | 64 |
| The conventional strain of embodiment 5 | 7 | 20 | 65 |
| The conventional strain of embodiment 6 | 7 | 20 | 66 |
| Embodiment 7 cultivates strain | 5 | 12 | 96 |
| Embodiment 8 cultivates strain | 5 | 12 | 96 |
| Embodiment 9 cultivates strain | 5 | 12 | 96 |
Result show through strengthening after resistance to nicotine Sarcophga fuscicauda kind after cultivating for 20 generations with cellar culture material, resistance to cigarette
Alkali ability there occurs degradation phenomena, and cultivates the resistance to nicotine Sarcophga fuscicauda lines progenies of acquisition to nicotine tolerance through the present invention
Stability fine, and in inventive formulation material compositing range, the formulation material of the same weight composition of different batches obtains
Sarcophga fuscicauda has stable cultivation effect.
Claims (7)
1. the breeding method of a resistance to nicotine Sarcophga fuscicauda strain, it is characterised in that comprise the steps:
S1., under indoor conditions, the Sarcophga fuscicauda kind of the nicotine aqueous formulation material that mass percent concentration is 3 ‰ will be tolerated
Newly hatched larvae be seeded in the first formulation material, by Sarcophga fuscicauda newly hatched larvae cultivate aging to larva, pupate；
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it produces newly hatched larvae, enters next domestication from generation to generation
Cultivate；
S3. by above-mentioned steps repetitive operation, cultivate through 20～30 continuous domestication from generation to generation, it is thus achieved that stable resistance to nicotine palm fibre tail is other
Sarcophagid strain；
Wherein, described first formulation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, uses
It is 60% that above-mentioned first formulation material is adjusted to humidity by the nicotine aqueous solution of mass percent concentration 3 ‰；
Second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7% and nicotine
0.3%.
Breeding method the most according to claim 1, it is characterised in that indoor conditions described in S1 is temperature 25～30 DEG C, wet
Degree 30～70%.
Breeding method the most according to claim 1, it is characterised in that the compound method of described first formulation material is for join first
After each component mixing of square stock, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned first formulation material being adjusted to humidity is
60%.
Breeding method the most according to claim 1, it is characterised in that access palm fibre tail among the first formulation material described in every 1kg other
Sarcophagid newly hatched larvae 0.5～1.0g.
Breeding method the most according to claim 1, it is characterised in that described tolerance mass percent concentration is the nicotine of 3 ‰
The preparation method of the Sarcophga fuscicauda kind of aqueous formulation material is: first filter out resistance to cigarette from wild Sarcophga fuscicauda kind
Alkali Sarcophga fuscicauda kind, carries out hot housing by the resistance to nicotine Sarcophga fuscicauda kind screened the most again so that it is tolerance
Mass percent concentration is the nicotine aqueous formulation material of 3 ‰.
Breeding method the most according to claim 5, it is characterised in that the side of the screening of described resistance to nicotine Sarcophga fuscicauda kind
Method is:
S1. configuration lures collection compost, and collection compost will be lured to load in open containers；
The described each component luring collection compost to contain following percetage by weight: wheat bran 80%, imported fish meal 20%, uses percent mass
Above-mentioned luring collect compost to be adjusted to humidity be 60% by the nicotine aqueous solution of specific concentration 1 ‰～2 ‰；
S2., when weather is fine, will be equipped with luring the container of collection compost to be put in house refuse heap, chicken house and pig farm respectively the morning and lure
Collecting wild Sarcophga fuscicauda newly hatched larvae, regain the container being put in everywhere in the afternoon；
S3. each container reclaimed is placed on indoor cultivation, waits the wild Sarcophga fuscicauda newly hatched larvae luring collection to arrive to cultivate to larva
Time aging, utilize being separated by fly larvae from food phase behavior of maggots of boettcherisca peregrine with luring collection compost；
S4. by the Morphological Identification of Sarcophga fuscicauda larva, maggots of boettcherisca peregrine is chosen；
S5. 4～5 days Sarcophga fuscicaudas aging, that every fly larvae weighs 0.06～0.08g, figure is plump, color is golden yellow are filtered out
Maggot, at ambient temperature, allow maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
Breeding method the most according to claim 5, it is characterised in that the hot housing of described resistance to nicotine Sarcophga fuscicauda kind
Method be:
Starting stage: the newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described initial incubation
Material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the cigarette of mass percent concentration 1 ‰
It is 60% that above-mentioned initial incubation material is adjusted to humidity by aqueous alkali；In room conditions, by above-mentioned Sarcophga fuscicauda newly hatched larvae
Cultivate aging to larva, pupate, sprout wings after, feed adult；
1st strain: after the adult until the starting stage produces newly hatched larvae, the newly hatched larvae produced uses the 1st time by force
Change compost and carry out hot housing；Described 1st hot housing material is made up of each component of following percetage by weight: wheat bran
80%, imported fish meal 20%, adjusts above-mentioned 1st hot housing material with the nicotine aqueous solution of mass percent concentration (1+S) ‰
Being 60% to humidity, S is selected from 0.1～0.5；In room conditions, above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated old to larva
Ripe, pupate, sprout wings after, feed adult；
The N+1 time strain: the newly hatched larvae of the adult product of n-th strain gained is carried out hot housing again, and
And component contained by the compost used by the N+1 time strain meets following ratio: the mass percent concentration of nicotine aqueous solution
=(content+S of nicotine in n-th hot housing material) ‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, weight
Multiple strengthening, till in compost described in last strain, the mass percent concentration of nicotine aqueous solution reaches 3 ‰.
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|---|---|---|---|
| CN201410420985.3A CN104285892B (en) | 2014-08-25 | A kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410420985.3A CN104285892B (en) | 2014-08-25 | A kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain |
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