CN104285897A - Method for strengthening nicotine-resisting hermetia illucens breeds - Google Patents
Method for strengthening nicotine-resisting hermetia illucens breeds Download PDFInfo
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- CN104285897A CN104285897A CN201410421787.9A CN201410421787A CN104285897A CN 104285897 A CN104285897 A CN 104285897A CN 201410421787 A CN201410421787 A CN 201410421787A CN 104285897 A CN104285897 A CN 104285897A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention discloses a method for strengthening nicotine-resisting hermetia illucens breeds. The method comprises the steps that spawns produced by the screened wild nicotine-resisting hermetia illucens breeds are bred into adults through initial breeding materials, and the spawns produced by the adults are strengthened N times through strengthening breeding materials and bred into adults. The initial breeding materials are composed of, by weight, 70 percent to 90 percent of bran and 10 percent to 30 percent of imported fish meal, and the humidity of the formula materials is adjusted to 70 percent through the nicotine aqueous solution with the mass percent concentration of one permillage. The nicotine aqueous solution with the proper concentration is directly and scientifically combined with the bran and the imported fish meal, hermetia illucens is successfully strengthened and bred, the nicotine resisting capacity of the hermetia illucens can be effectively and gradually improved, severe dispause of the hermetia illucens cannot be caused, the nicotine resisting capacity of the wild hermetia illucens breeds can be strengthened step by step, and the more stable breeding effect is obtained.
Description
Technical field
The present invention relates to animal varieties triage techniques field, be specifically related to a kind of intensifying method of the black soldier flies of resistance to nicotine kind.
Background technology
Inapplicable fresh tobacco leaf refers to the fresh tobacco leaves (water content 80 ~ 90%) not being suitable for cured tobacco production, different from the waste/hypo-tobacco leaf produced in the production process of flue-cured tobacco mentioned in a large number in document or discarded tobacco leaf (water content about 15%).Inapplicable tobacco leaf typically refers to cigarette strain and normally to bind and after removing 2 ~ 3 milk pin leaves, cigarette industry is inapplicable, need remove inferior leads and the upper leaf of process in field, mainly to comprise bottom cigarette strain because of illumination deficiency, depauperation, inclusion be not enough, estimate roasting after inferior quality, do not possess 2 inferior leads that baking is worth, and cigarette strain top hack bad, curtailment, structure are stiff, roasting after 2 tops of inferior quality.
If these inapplicable tobacco leaves arbitrarily abandon field, very easily cause the superinfection of environmental pollution and tobacco virus, then need to spend a large amount of expenses according to the concentrated destruction of prior art or the processing mode of landfill, the disposal cost of current industry inapplicable tobacco leaf per ton is on average up to 200 yuan, each tobacco leaf producing region often needs under the organization leadership of government of local party committee, carry out policy propaganda, tabulation registration, build treatment facility, unified to remove, record file, publicity, cash the measures such as subsidy and supervise the process carrying out inapplicable tobacco leaf, waste time and energy costly.
Adopting the insect of resistance to nicotine to carry out biological treatment to inapplicable tobacco leaf is a kind of alternate process method preferably, environmental protection, high financial profit.In order to utilize insect to realize the inapplicable fresh tobacco leaf of biological treatment, first need the insect species filtering out resistance to nicotine.
The applicant, based on studying for a long period of time to housefly and inapplicable fresh tobacco leaf treatment technology, sums up and utilizes inapplicable fresh tobacco leaf to carry out the method for the screening of resistance to nicotine strain, strengthening and cultivation to housefly.Described strengthening the ovum of the resistance to nicotine housefly filtered out is inoculated in inapplicable fresh tobacco leaf composts or fertilisers of cultivating be cultured to adult, inapplicable fresh tobacco leaf hot housing material is utilized by the ovum of adult output to carry out N hot housing to adult again, the ratio that in each hot housing circulation, inapplicable fresh tobacco leaf accounts for composts or fertilisers of cultivating all can improve 5 ~ 15 points on the basis of upper once hot housing material, until the ratio of inapplicable fresh tobacco leaf in hot housing material reaches 85%, namely the housefly kind that this stage turns out has higher tolerance to nicotine.Described inapplicable fresh tobacco leaf composts or fertilisers of cultivating is containing, for example each component of lower percetage by weight: inapplicable fresh tobacco leaf 30%, wheat bran 60%, imported fish meal 5%, dregs of beans 5%
This technical scheme can go out the housefly kind of resistance to nicotine by successful incubation, but the resistance to nicotine ability that different batches cultivates the aging prepupa obtained also is distinguished to some extent, there is the uncertainty of effect.Main cause is the application aspect of inapplicable tobacco leaf, although sum up in formulation material through creationary research and great many of experiments the inapplicable tobacco leaf adding zone of reasonableness can reduce the toxic action of nicotine to common house-fly grub, but the factor affecting inapplicable fresh tobacco leaf nicotine content is very various, complicated mechanism, more difficult realization accurately controls.The factor affecting nicotine content in tobacco leaf mainly comprises: (1) different growing stages, in tobacco leaf, contained nicotine content is different: in tobacco seed, nicotine content is 0, rise in seedling raising process and start to be formed at root, after developing along with root system, synthesis and the accumulation ability of nicotine strengthen gradually, so different growing stages, in tobacco leaf, contained nicotine content is different; (2) cigarette strain different parts, nicotine content is significantly different, and nicotine content is also by leaf position and the larger impact of the number of sheets; (3) nicotine amount contained by different cultivars tobacco leaf is different; (4) weather conditions etc. of soil dry-wet all have the impact of different savings on nicotine content; (5) difference of agronomic measures significantly affects nicotine content of tobacco leaves, comprise fertilization type, planting density, pinch smear wooden fork fertilising, girdling and root fertilizer, picking methods etc. factor of cutting all significantly affect nicotine content in tobacco leaf, for fertilization type, in the nitrogenous fertilizer of three kinds of forms, after using urea, nicotine accumulation is the highest, and ammonium nitrate takes second place, the nicotine accumulation of potassium nitrate process is minimum, lower by 27% than using urea; For Physical property, Zhang Dan etc. study discovery, and postpone nicotine accumulation intensity peak with Physical property and decline successively, nicotine content also lowers successively, and often postpone and pinching for one day, nicotine content just declines 0.0504%; Again to cut root fertilizer, cut root 1/8 and do not cut compared with root, upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
The uncertainty adopting different batches composts or fertilisers of cultivating to cultivate the aging prepupa effect obtained how is avoided to be this area technical barrier urgently to be resolved hurrily.And adopt and stablize feasible technical scheme and cultivate the insect strain of resistance to nicotine of more multi items and carry out reinforced cultivating effectively, be the important technological problems that this area needs to solve simultaneously.
Summary of the invention
The technical problem to be solved in the present invention is to overcome in prior art the defect lacked the strong black soldier flies of the resistance to nicotine kind of the tolerance of nicotine, provides a kind of intensifying method of the black soldier flies of resistance to nicotine kind.
Object of the present invention is achieved by the following technical programs:
Black soldier flies is the another kind of insect being different from housefly, and it is a kind of important economic insects and scavenger insect, can utilize the various corrupt organic waste of black soldier flies larva process, as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc.Existing black soldier flies kind all has good biological treatment capabilities to nontoxic organic waste, but not good to the treatment effect of inapplicable fresh tobacco leaf.Main cause is because containing toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, have certain murder by poisoning and the effect of Developing restraint to black soldier flies larva (black soldier flies prepupa).In order to utilize black soldier flies to carry out a biological disposal upon inapplicable fresh tobacco leaf, first need filter out the black soldier flies kind of resistance to nicotine from wild black soldier flies kind, improving black soldier flies to the tolerance of fresh tobacco leaf.
The invention provides a kind of intensifying method of the black soldier flies of resistance to nicotine kind, comprise the steps:
Starting stage: the ovum of the black soldier flies of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material is containing, for example each component of lower percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%; In room conditions, above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, utilize clear water to feed its adult;
1st strain: after the Adult worms producting eggs of starting stage, the ovum produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1 ~ 0.5; In room conditions, above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult;
The N+1 time strain: again hot housing is carried out to the ovum that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: the mass percent concentration of the nicotine aqueous solution=content+S of nicotine (in the N time hot housing material) ‰, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.Namely the black soldier flies kind that this stage turns out has higher tolerance to nicotine.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 20 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 10 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 4 hot housing altogether.
The described black soldier flies of resistance to nicotine kind refers to the black soldier flies of the resistance to nicotine kind filtered out from wild black soldier flies kind.
The described concrete grammar filtering out the black soldier flies of resistance to nicotine kind from wild black soldier flies kind is:
S1. configuration lures collection composts or fertilisers of cultivating, and collection composts or fertilisers of cultivating will be lured to load in open containers;
The described collection composts or fertilisers of cultivating that lures is containing, for example each component of lower percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰ ~ 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
S2., when weather is fine, by being equipped with, lure that the container of collection composts or fertilisers of cultivating is put in house refuse heap respectively, chicken house and pig farm lure the wild worm's ovum of collection in the morning (preferred 8:00), regain the container be put in everywhere in the afternoon (preferred 17:00);
S3. each container reclaimed is placed on indoor cultivation, when the wild worm's ovum arrived etc. luring collection is cultivated aging to larva, utilize it being separated with formulation material (can climb from composts or fertilisers of cultivating voluntarily when namely black soldier flies prepupa is aging, find dry place and pupate) from food phase behavior of black soldier flies prepupa;
S4. by the Morphological Identification of black soldier flies larva, black soldier flies prepupa is chosen;
S5. filter out the black soldier flies prepupa that 20 ~ 25 days aging, every bar prepupa weigh 0.10 ~ 0.16 g, figure is plump, color is pitch-black, at ambient temperature, allow black soldier flies prepupa naturally pupate, sprout wings after can obtain the black soldier flies kind of resistance to nicotine.
Preferably, described indoor conditions is temperature 25 ~ 30 DEG C, humidity 30 ~ 70%.
The collocation method of described initial incubation material is that after each component mixing by initial incubation material, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Preferably, black soldier flies ovum 0.5 ~ 1.0 g is accessed in every 1 kg initial incubation material; Black soldier flies ovum 0.5 ~ 1.0 g is accessed in every 1 kg hot housing material.
Its adult of described nursing is all utilize water to feed.Preferably, described clear water can select pure water, running water, well water or nontoxic river etc.; The mode that water is fed utilizes sponge full of water, sucks for adult.
The invention has the beneficial effects as follows:
By science determination proportion compatibility, the present invention creatively directly adopts the nicotine aqueous solution of suitable concentration and wheat bran and imported fish meal scientific compatibility, the first formulation material obtained not only successfully covers the unpleasant of nicotine and bitter taste, nutrient component is suitable, and the reasonability configured before and after nutriment contained by initial incubation material and hot housing material and nicotine equal size, ensure that black soldier flies larval growth is smooth, and because the appropriateness of nicotine content and certainty, guarantee in same nurturing an environment, the formulation material of the same weight composition of different batches obtains stable way to cultivate.
By hot housing method of the present invention, both the tolerance of black soldier flies kind to nicotine effectively can progressively be improved, be unlikely to again to cause serious diapause to black soldier flies kind, can strengthen wild black soldier flies kind by easy stages to the tolerance of nicotine, in composts or fertilisers of cultivating, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Embodiment
The present invention is further described below in conjunction with specific embodiment.Unless stated otherwise, the present invention adopts reagent, equipment are the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
embodiment 1the black soldier flies of resistance to nicotine kind is filtered out from wild black soldier flies kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild worm's ovum arrived luring collection is cultivated aging to larva, utilizes being separated with formulation material by prepupa (can climb from composts or fertilisers of cultivating voluntarily when namely black soldier flies prepupa is aging, find dry place and pupate) from food phase behavior of black soldier flies prepupa.
S04. under anatomical lens by the Morphological Identification of larva, choose separately black soldier flies prepupa with tweezers, get rid of other fly kinds.
S05. filter out the black soldier flies prepupa that 20 ~ 25 days aging, every bar prepupa weigh 0.10 ~ 0.16 g, figure is plump, color is pitch-black, at ambient temperature, allow these black soldier flies prepupa naturally pupate, sprout wings after can obtain the black soldier flies kind of resistance to nicotine.
embodiment 2the black soldier flies of resistance to nicotine kind is filtered out from wild black soldier flies kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild worm's ovum arrived luring collection is cultivated aging to larva, utilizes being separated with formulation material by prepupa (can climb from composts or fertilisers of cultivating voluntarily when namely black soldier flies prepupa is aging, find dry place and pupate) from food phase behavior of black soldier flies prepupa.
S04. under anatomical lens by the Morphological Identification of larva, choose separately black soldier flies prepupa with tweezers, get rid of other fly kinds.
S05. filter out the black soldier flies prepupa that 20 ~ 25 days aging, every bar prepupa weigh 0.10 ~ 0.16 g, figure is plump, color is pitch-black, at ambient temperature, allow these black soldier flies prepupa naturally pupate, sprout wings after can obtain the black soldier flies kind of resistance to nicotine.
embodiment 3the black soldier flies of resistance to nicotine kind is filtered out from wild black soldier flies kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild worm's ovum arrived luring collection is cultivated aging to larva, utilizes being separated with formulation material by prepupa (can climb from composts or fertilisers of cultivating voluntarily when namely black soldier flies prepupa is aging, find dry place and pupate) from food phase behavior of black soldier flies prepupa.
S04. under anatomical lens by the Morphological Identification of larva, choose separately black soldier flies prepupa with tweezers, get rid of other fly kinds.
S5. filter out the black soldier flies prepupa that 20 ~ 25 days aging, every bar prepupa weigh 0.10 ~ 0.16 g, figure is plump, color is pitch-black, at ambient temperature, allow these black soldier flies prepupa naturally pupate, sprout wings after can obtain the black soldier flies kind of resistance to nicotine.
embodiment 4hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the black soldier flies of the resistance to nicotine kind screened
The black soldier flies of the resistance to nicotine kind that embodiment 1 ~ 3 any embodiment screens cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain: again hot housing is carried out to the ovum that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 20 hot housing altogether.Namely the black soldier flies kind that this stage turns out has higher tolerance to nicotine.
embodiment 5hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the black soldier flies of the resistance to nicotine kind screened
The black soldier flies of the resistance to nicotine kind that embodiment 1 ~ 3 any embodiment screens cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: again hot housing is carried out to the ovum that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 10 hot housing altogether.Namely the black soldier flies kind that this stage turns out has higher tolerance to nicotine.
embodiment 6hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the black soldier flies of the resistance to nicotine kind screened
The black soldier flies of the resistance to nicotine kind that embodiment 1 ~ 3 any embodiment screens cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: again hot housing is carried out to the ovum that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰; Also namely black soldier flies ovum experiences 4 hot housing altogether.Namely the black soldier flies kind that this stage turns out has higher tolerance to nicotine.
comparative example 1
Operation, with embodiment 1, is made up of each component of following percetage by weight unlike the described collection composts or fertilisers of cultivating that lures: wheat bran 80% and imported fish meal 20%.Experiment find to utilize as above lure collection composts or fertilisers of cultivating lure collection to ovum be not the kind of resistance to nicotine.
embodiment 7
The black soldier flies kind that the embodiment of the present invention 1 to 3 is screened, embodiment 4 to 6 through cultivating the black soldier flies kind of nicotine formulation material of tolerance 3 ‰, comparative example 1 process after black soldier flies kind carry out resistance to nicotine aptitude tests, test process is:
Get the ovum 0.5g that the black soldier flies adult after described embodiment cultivation process produces respectively, access formulation material (wheat bran 80%, imported fish meal 20% containing the better quality percent concentration nicotine aqueous solution that 1kg is heavy respectively, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 70%) in, test result is shown in Table 1:
Table 1
| Process | Larvae development go through the phase (my god) | Larva number/g(bar) | Survival rate of larvae (%) |
| Embodiment 1 is initially planted | 28 | 14 | 64 |
| Embodiment 2 is initially planted | 28 | 12 | 68 |
| Embodiment 3 is initially planted | 28 | 13 | 65 |
| Comparative example 1 | 35 | 22 | 14 |
| Embodiment semi-finals kind | 21 | 8 | 96 |
| Embodiment 5 strengthening kind | 23 | 9 | 94 |
| Embodiment 6 strengthening kind | 24 | 10 | 93 |
Test result illustrates, comparative example 1 can not tolerate the nicotine aqueous solution of mass percent concentration 3.0 ‰, and black soldier flies larva can not enter prepupa state.
There occurs diapause phenomenon in the formulation material that embodiment 1 to 3 modulate at the nicotine aqueous solution of mass percent concentration 3.0 ‰ through the black soldier flies kind of screening, pre-pupal weight comparatively gently, and has relatively large dead larvae.The resistance to nicotine ability of the black soldier flies kind of embodiment 4 to 6 after the inventive method hot housing process improves greatly, can tolerate the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰.
Claims (7)
1. an intensifying method for the black soldier flies of resistance to nicotine kind, is characterized in that, comprises the steps:
Starting stage: the ovum of the black soldier flies of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material is made up of the component of following weight fraction: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%; In room conditions, above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult;
1st strain: after the Adult worms producting eggs of starting stage, the ovum produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is made up of each component of following weight fraction: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1 ~ 0.5; In room conditions, above-mentioned worm's ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult;
The N+1 time strain: again hot housing is carried out to the ovum that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: the mass percent concentration of the nicotine aqueous solution=content+S of nicotine (in the N time hot housing material) ‰, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
2. intensifying method according to claim 1, it is characterized in that, described indoor conditions is temperature 25 ~ 30 DEG C, humidity 30 ~ 70%.
3. intensifying method according to claim 1, is characterized in that, the collocation method of described initial incubation material is that after each component mixing by initial incubation material, the humidity of composts or fertilisers of cultivating is adjusted to 70% by the nicotine aqueous solution adding certain concentration.
4. intensifying method according to claim 1, is characterized in that, access black soldier flies ovum 0.5 ~ 1.0g in every 1kg initial incubation material; Black soldier flies ovum 0.5 ~ 1.0g is accessed in every 1 kg hot housing material.
5. breeding method according to claim 1, it is characterized in that, its adult of described nursing utilizes water to feed.
6. intensifying method according to claim 1 or 5, is characterized in that, described water is pure water, running water, well water or nontoxic river; The described mode utilizing water to feed adopts sponge full of water, sucks for adult.
7. intensifying method according to claim 1, it is characterized in that, the screening technique of the described black soldier flies of resistance to nicotine kind is:
S1. configuration lures collection composts or fertilisers of cultivating, and collection composts or fertilisers of cultivating will be lured to load in open containers;
The described collection composts or fertilisers of cultivating that lures is made up of each component of following weight fraction: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰ ~ 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
S2., when weather is fine, by being equipped with, lure that the container of collection composts or fertilisers of cultivating is put in house refuse heap respectively, chicken house and pig farm lure the wild worm's ovum of collection in the morning, regain the container be put in everywhere in the afternoon;
S3. each container reclaimed is placed on indoor cultivation, when waiting the wild worm's ovum luring collection to arrive to cultivate aging to larva, utilizes being separated with formulation material by prepupa from food phase behavior of black soldier flies prepupa;
S4. by the Morphological Identification of black soldier flies larva, black soldier flies prepupa is chosen;
S5. filter out the black soldier flies prepupa that 20 ~ 25 days aging, every bar prepupa weigh 0.10 ~ 0.16g, figure is plump, color is pitch-black, at ambient temperature, allow black soldier flies prepupa naturally pupate, sprout wings after can obtain the black soldier flies kind of resistance to nicotine.
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| KR101878020B1 (en) * | 2015-08-24 | 2018-07-13 | 씨제이제일제당 (주) | Feed composition for blacksoldier fly and use thereof |
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