CN104285892A - Cultivation method of nicotine-resistant boettcherisca peregrina strain - Google Patents
Cultivation method of nicotine-resistant boettcherisca peregrina strain Download PDFInfo
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- CN104285892A CN104285892A CN201410420985.3A CN201410420985A CN104285892A CN 104285892 A CN104285892 A CN 104285892A CN 201410420985 A CN201410420985 A CN 201410420985A CN 104285892 A CN104285892 A CN 104285892A
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- nicotine
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- sarcophga fuscicauda
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 175
- 229960002715 nicotine Drugs 0.000 title claims abstract description 175
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 175
- 241000257193 Sarcophaga peregrina Species 0.000 title claims abstract description 30
- 238000012364 cultivation method Methods 0.000 title abstract 4
- 239000000463 material Substances 0.000 claims abstract description 126
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 33
- 239000004467 fishmeal Substances 0.000 claims abstract description 33
- 239000000203 mixture Substances 0.000 claims description 93
- 238000009472 formulation Methods 0.000 claims description 88
- 239000007864 aqueous solution Substances 0.000 claims description 52
- 239000002361 compost Substances 0.000 claims description 45
- 235000015099 wheat brans Nutrition 0.000 claims description 30
- 230000032683 aging Effects 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 22
- 238000009395 breeding Methods 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 16
- 235000008939 whole milk Nutrition 0.000 claims description 16
- 238000011534 incubation Methods 0.000 claims description 11
- 239000013011 aqueous formulation Substances 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000005728 strengthening Methods 0.000 claims description 7
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 5
- 241000382353 Pupa Species 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 5
- 230000000474 nursing effect Effects 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- FFYVQVZXWDGRAY-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;hydrate Chemical compound O.CN1CCCC1C1=CC=CN=C1 FFYVQVZXWDGRAY-UHFFFAOYSA-N 0.000 abstract 2
- 239000004615 ingredient Substances 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 241000208125 Nicotiana Species 0.000 description 31
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 31
- 230000008569 process Effects 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 241000238631 Hexapoda Species 0.000 description 8
- 241000257159 Musca domestica Species 0.000 description 8
- 238000012360 testing method Methods 0.000 description 6
- 235000019504 cigarettes Nutrition 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a cultivation method of a nicotine-resistant boettcherisca peregrina strain. According to the cultivation method, early larvae of a nicotine-resistant boettcherisca peregrina variety are inoculated in a first formula material containing nicotine and cultivated till the larvae mature and pupate; after pupae emerge, a second formula material is used for feeding the pupae into imagoes; after the imagoes lay early larvae, the stable nicotine-resistant boettcherisca peregrina strain can be obtained by means of continuous acclimation and cultivation over 20-30 generations. The first formula material is composed of, by weight, 70-90% of bran and 10-30% of imported fish meal, wherein the humidity of the bran and the imported fish meal is adjusted to be 60% in a nicotine water solution with the mass percent concentration of 0.3%. By means of the cultivation method, the nicotine water solution with the proper concentration is directly adopted for scientific compatibility with the bran and the imported fish meal, the obtained first formula material successfully covers the bad smell and bitterness of the nicotine, nutritional ingredients are appropriate, smooth growth of the larvae of boettcherisca peregrina is guaranteed, and it is ensured that a stable cultivation effect is achieved.
Description
Technical field
The present invention relates to insect Cultivating techniques field, be specifically related to a kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain.
Background technology
Inapplicable fresh tobacco leaf refers to the fresh tobacco leaves (water content 80 ~ 90%) not being suitable for cured tobacco production, different from the waste/hypo-tobacco leaf produced in the production process of flue-cured tobacco mentioned in a large number in document or discarded tobacco leaf (water content about 15%).Inapplicable tobacco leaf typically refers to cigarette strain and normally to bind and after removing 2 ~ 3 milk pin leaves, cigarette industry is inapplicable, need remove inferior leads and the upper leaf of process in field, mainly to comprise bottom cigarette strain because of illumination deficiency, depauperation, inclusion be not enough, estimate roasting after inferior quality, do not possess 2 inferior leads that baking is worth, and cigarette strain top hack bad, curtailment, structure are stiff, roasting after 2 tops of inferior quality.
If these inapplicable tobacco leaves arbitrarily abandon field, very easily cause the superinfection of environmental pollution and tobacco virus, then need to spend a large amount of expenses according to the concentrated destruction of prior art or the processing mode of landfill, the disposal cost of current industry inapplicable tobacco leaf per ton is on average up to 200 yuan, each tobacco leaf producing region often needs under the organization leadership of government of local party committee, carry out policy propaganda, tabulation registration, build treatment facility, unified to remove, record file, publicity, cash the measures such as subsidy and supervise the process carrying out inapplicable tobacco leaf, waste time and energy costly.
Adopting the insect of resistance to nicotine to carry out biological treatment to inapplicable tobacco leaf is a kind of alternate process method preferably, environmental protection, high financial profit.In order to utilize insect to realize the inapplicable fresh tobacco leaf of biological treatment, first need the insect species filtering out resistance to nicotine.
The applicant, based on studying for a long period of time to housefly and inapplicable tobacco leaf treatment technology, sums up and utilizes inapplicable tobacco leaf to carry out the screening of resistance to nicotine strain, strengthening and breeding method to housefly.Described cultivation is that the newly hatched larvae of resistance to nicotine housefly is inoculated in the first formulation material, and fly pupa feeds adult with the second formulation material after sprouting wings, and the continuous domestication through 20 ~ 30 generations obtains resistance to nicotine housefly kind.The key of its method is that the composition of the first formulation material and the second formulation material is to ensure the smooth growth of resistance to nicotine housefly.First formulation material consist of inapplicable fresh tobacco leaf 75 ~ 80%, wheat bran 10 ~ 15%, imported fish meal 5 ~ 10%; Second formulation material is whole milk powder 70%, brown sugar 29.8 ~ 29.9%, pure nicotine 0.1 ~ 0.2%.
This technical scheme can go out the housefly kind of resistance to nicotine by successful incubation, but the quantity difference that different batches cultivates the aging fly maggot obtained is comparatively large, and resistance to nicotine ability is also distinguished to some extent, there is the uncertainty of effect.Main cause is the application aspect of inapplicable tobacco leaf, although sum up in formulation material through creationary research and great many of experiments the control that the inapplicable tobacco leaf adding zone of reasonableness can ensure common house-fly grub toxic action, but the factor affecting not nicotine content of tobacco leaves is very various, complicated mechanism, more difficult realization accurately controls.The factor affecting nicotine content in tobacco leaf mainly comprises: (1) different growing stages, in tobacco leaf, contained nicotine content is different: in tobacco seed, nicotine content is 0, rise in seedling raising process and start to be formed at root, after developing along with root system, synthesis and the accumulation ability of nicotine strengthen gradually, so different growing stages, in tobacco leaf, contained nicotine content is different; (2) cigarette strain different parts, nicotine content is significantly different, and nicotine content is also by leaf position and the larger impact of the number of sheets; (3) nicotine amount contained by different cultivars tobacco leaf is different; (4) weather conditions etc. of soil dry-wet all have the impact of different savings on nicotine content; (5) difference of agronomic measures significantly affects nicotine content of tobacco leaves, comprise fertilization type, planting density, pinch smear wooden fork fertilising, girdling and root fertilizer, picking methods etc. factor of cutting all significantly affect nicotine content in tobacco leaf, for fertilization type, in the nitrogenous fertilizer of three kinds of forms, after urea is used, nicotine accumulation is the highest, and ammonium nitrate takes second place, the nicotine accumulation of potassium nitrate process is minimum, lower by 27% than using urea; For Physical property, Zhang Dan etc. study discovery, and postpone nicotine accumulation intensity peak with Physical property and decline successively, nicotine content also lowers successively, and often postpone and pinching for one day, nicotine content just declines 0.0504%; Again to cut root fertilizer, cut root 1/8 and do not cut compared with root, upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.The uncertainty adopting different batches composts or fertilisers of cultivating to cultivate the aging fly maggot effect obtained how is avoided to be this area technical barrier urgently to be resolved hurrily.And adopt stablize feasible technical scheme cultivate more multi items the insect strain of resistance to nicotine and effectively continuous domestication make its stablize, be this area need simultaneously solve important technological problems.
Summary of the invention
The technical problem to be solved in the present invention is to overcome in prior art the defect lacking resistance to nicotine Sarcophga fuscicauda kind, a kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain is provided, the inventive method does not affect by inapplicable tobacco leaf various factors, can obtain stable way to cultivate.
Object of the present invention is achieved by the following technical programs:
Sarcophga fuscicauda is a kind of insect being different from housefly, and it is a kind of important economic insects and scavenger insect, can utilize the various corrupt organic waste of Sarcophga fuscicauda process, as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc.Existing Sarcophga fuscicauda kind all has good biological treatment capabilities to nontoxic organic waste, but not good to the treatment effect of inapplicable fresh tobacco leaf.Main cause is because containing toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, have certain murder by poisoning and the effect of Developing restraint to Sarcophga fuscicauda larva (maggots of boettcherisca peregrine).In order to utilize Sarcophga fuscicauda to carry out a biological disposal upon inapplicable fresh tobacco leaf, first need filter out the Sarcophga fuscicauda kind of resistance to nicotine from wild Sarcophga fuscicauda kind, improving Sarcophga fuscicauda to the tolerance of fresh tobacco leaf.
The invention provides a kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain, comprise the steps:
S1. be that the newly hatched larvae of the Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ is seeded in the first formulation material by tolerance mass percent concentration under indoor conditions, Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate;
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and produces after newly hatched larvae until it, enters the acclimatization culture of next generation;
S3. repeat by above-mentioned steps, the continuous domestication through 20 ~ 30 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain;
Wherein, described first formulation material is made up of each component of following percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%;
Second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7 ~ 29.8% and nicotine 0.2 ~ 0.3%.
Preferably, described first formulation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
Preferably, described second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7% and nicotine 0.3%.
Described nicotine can adopt the commercial products of purity >=99.5%.
The compound method of described first formulation material is that after each component mixing by the first formulation material, the humidity of formulation material is adjusted to 60% by the nicotine aqueous solution adding extra fine quality percent concentration.
Sarcophga fuscicauda newly hatched larvae 0.5 ~ 1.0 g is accessed among described every 1 kg first formulation material.Sarcophga fuscicauda newly hatched larvae 0.8 g is accessed in preferred described every 1 kg first formulation material.
Described tolerance mass percent concentration is that the resistance to nicotine Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ refers to and first from wild Sarcophga fuscicauda kind, filters out resistance to nicotine Sarcophga fuscicauda kind, and then the resistance to nicotine Sarcophga fuscicauda kind screened is carried out hot housing, make it tolerate nicotine aqueous formulation material that mass percent concentration is 3 ‰.
The described concrete grammar filtering out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind is:
S01. configuration lures collection composts or fertilisers of cultivating, and collection composts or fertilisers of cultivating will be lured to load in open containers;
The described collection composts or fertilisers of cultivating that lures is containing, for example each component of lower percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰ ~ 2 ‰, above-mentioned formulation material being adjusted to humidity is 60%;
S02., when weather is fine, the morning by being equipped with, 8:00 ~ 9:00 lures that the container of collection composts or fertilisers of cultivating is put in house refuse heap respectively, chicken house and pig farm lure the wild Sarcophga fuscicauda newly hatched larvae of collection, and afternoon, 17:00 ~ 18:00 regained the container be put in everywhere;
S03. each container reclaimed is placed on indoor cultivation, when waiting the wild Sarcophga fuscicauda newly hatched larvae luring collection to arrive to cultivate aging to larva, utilizes being separated with formulation material by fly maggot from food phase behavior of maggots of boettcherisca peregrine;
S04. by the Morphological Identification of Sarcophga fuscicauda larva, maggots of boettcherisca peregrine is chosen;
S05. filter out 4 ~ 5 days aging, every bar fly maggots and weigh 0.06 ~ 0.08 g, figure is plump, color is golden yellow maggots of boettcherisca peregrine, at ambient temperature, allow maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
Described the resistance to nicotine Sarcophga fuscicauda kind screened is carried out hot housing, to make it tolerate mass percent concentration be the concrete grammar of the nicotine aqueous formulation material of 3 ‰ is:
S11. the starting stage: the newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%; In room conditions, above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S12. the 1st strain: after the adult of S11 produces newly hatched larvae, the newly hatched larvae produced adopts the 1st hot housing material to carry out cultivation and cultivates; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, above-mentioned formulation material being adjusted to humidity with the nicotine aqueous solution of mass percent concentration (1+S) ‰ is that the value of 60%, S is selected from 0.1 ~ 0.5; In room conditions, above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: again hot housing is carried out to the newly hatched larvae that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last breeding phase, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Its adult of described nursing is all utilize the mixture of whole milk powder, brown sugar and water to feed.Preferably, according to percent by weight, the usage ratio of described whole milk powder, brown sugar is whole milk powder 70%, brown sugar 30%; The mode that water is fed utilizes sponge full of water, sucks for adult.
Preferably, the indoor conditions of described cultivation Sarcophga fuscicauda is temperature 25 ~ 30 DEG C, humidity 30 ~ 70%.
The invention has the beneficial effects as follows:
The present invention creatively directly adopts the nicotine aqueous solution of suitable concentration and wheat bran and imported fish meal scientific compatibility, the first formulation material obtained not only successfully covers the unpleasant of nicotine and bitter taste, nutrient component is suitable, ensure that Sarcophga fuscicauda larval growth is smooth, and because the appropriateness of nicotine content and certainty, and the reasonability that first arranges before and after formulation material and the second formulation material nutriment and nicotine content, guarantee in same nurturing an environment, the maggots of boettcherisca peregrine rate that the formulation material of the same weight composition of different batches obtains is substantially identical, obtain stable way to cultivate.
The resistance to nicotine Sarcophga fuscicauda strain of acquisition is cultivated owing to have passed through the long-term acclimatization culture in 20 ~ 30 generations through the present invention, not only adapt to culture conditions, and define more stable genetic character, the stability of the resistance to nicotine ability of its offspring is fine, and easily degenerates without resistance to nicotine Sarcophga fuscicauda strain (only through the Sarcophga fuscicauda of screening and the hot housing) tolerance of offspring to nicotine that the present invention cultivates acquisition.
Embodiment
The present invention is further described below in conjunction with specific embodiment.Unless stated otherwise, the present invention adopts reagent, equipment are the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, and purity is >=99.5%.
embodiment 1resistance to nicotine Sarcophga fuscicauda kind is filtered out from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae arrived luring collection is cultivated aging to larva, utilize being separated with formulation material by fly maggot (can climb from composts or fertilisers of cultivating voluntarily when namely maggots of boettcherisca peregrine is aging, find dry place and pupate) from food phase behavior of maggots of boettcherisca peregrine.
S04. under anatomical lens by the Morphological Identification of larva, choose maggots of boettcherisca peregrine separately with tweezers, get rid of other fly kinds.
S05. filter out 4 ~ 5 days aging, every bar fly maggots and weigh 0.06 ~ 0.08 g, figure is plump, color is golden yellow maggots of boettcherisca peregrine, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
embodiment 2resistance to nicotine Sarcophga fuscicauda kind is filtered out from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae arrived luring collection is cultivated aging to larva, utilize being separated with formulation material by fly maggot (can climb from composts or fertilisers of cultivating voluntarily when namely maggots of boettcherisca peregrine is aging, find dry place and pupate) from food phase behavior of maggots of boettcherisca peregrine.
S04. under anatomical lens by the Morphological Identification of larva, choose maggots of boettcherisca peregrine separately with tweezers, get rid of other fly kinds.
S05. filter out 4 ~ 5 days aging, every bar fly maggots and weigh 0.06 ~ 0.08 g, figure is plump, color is golden yellow maggots of boettcherisca peregrine, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
embodiment 3resistance to nicotine Sarcophga fuscicauda kind is filtered out from wild Sarcophga fuscicauda kind
S01. when weather is fine, preparation lure collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating be made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm).In the morning on the same day 8:00 plastic basin that composts or fertilisers of cultivating is housed is put in by a life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (temperature 25 DEG C in room conditions, humidity 30%), the wild Sarcophga fuscicauda newly hatched larvae arrived luring collection is cultivated aging to larva, utilize being separated with formulation material by fly maggot (can climb from composts or fertilisers of cultivating voluntarily when namely maggots of boettcherisca peregrine is aging, find dry place and pupate) from food phase behavior of maggots of boettcherisca peregrine.
S04. under anatomical lens by the Morphological Identification of larva, choose maggots of boettcherisca peregrine separately with tweezers, get rid of other fly kinds.
S5. filter out 4 ~ 5 days aging, every bar fly maggots and weigh 0.06 ~ 0.08 g, figure is plump, color is golden yellow maggots of boettcherisca peregrine, at ambient temperature, allow these maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
embodiment 4hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the resistance to nicotine Sarcophga fuscicauda kind screened
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1 ~ 3 any embodiment screened is cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access Sarcophga fuscicauda newly hatched larvae 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult.Its adult of described nursing utilizes the mixture of whole milk powder, brown sugar and water to feed.Preferably, according to percent by weight, the usage ratio of described whole milk powder, brown sugar is whole milk powder 70%, brown sugar 30%; The mode that water is fed utilizes sponge full of water, sucks for adult.Lower same.
Strain:
S12. the 1st strain: after the adult of starting stage produces newly hatched larvae, the newly hatched larvae produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain: again hot housing is carried out to the newly hatched larvae that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰; Also namely Sarcophga fuscicauda newly hatched larvae experiences 20 hot housing altogether.Namely the Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
embodiment 5hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the resistance to nicotine Sarcophga fuscicauda kind screened
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1 ~ 3 any embodiment screened is cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access Sarcophga fuscicauda newly hatched larvae 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the adult of starting stage produces newly hatched larvae, the newly hatched larvae produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: again hot housing is carried out to the newly hatched larvae that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰; Also namely Sarcophga fuscicauda newly hatched larvae experiences 10 hot housing altogether.Namely the Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
embodiment 6hot housing make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰ is carried out to the resistance to nicotine Sarcophga fuscicauda kind screened
Resistance to nicotine Sarcophga fuscicauda kind embodiment 1 ~ 3 any embodiment screened is cultivated, make it tolerate formulation material that mass percent concentration is the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned composts or fertilisers of cultivating modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access Sarcophga fuscicauda newly hatched larvae 0.8 g; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the adult of starting stage produces newly hatched larvae, the newly hatched larvae produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is containing, for example each component of lower percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, S is selected from 0.1 ~ 0.5; In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: again hot housing is carried out to the newly hatched larvae that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: mass percent concentration=(the cultivating the content+S of nicotine in composts or fertilisers of cultivating for the N time) ‰ of the nicotine aqueous solution, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰; Also namely Sarcophga fuscicauda newly hatched larvae experiences 4 hot housing altogether.Namely the Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
embodiment 7the continuous domestication of multiple generation is cultivated and is obtained stable resistance to nicotine Sarcophga fuscicauda strain
Be that the Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ is cultivated through the continuous domestication of multiple generation by the tolerance mass percent concentration that embodiment 4 ~ 6 any embodiment cultivates again, form stable resistance to nicotine Sarcophga fuscicauda strain.Concrete grammar is:
S1., under indoor conditions, be that the newly hatched larvae of the Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ is seeded in the first formulation material by tolerance mass percent concentration, Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate;
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and produces after newly hatched larvae until it, enters the acclimatization culture of next generation;
S3. repeat by above-mentioned steps, the continuous domestication through 30 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain;
The preparation of the first formulation material: described first formulation material is made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
By the above-mentioned formulation material modulated, every 1 kg loads in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access Sarcophga fuscicauda newly hatched larvae 0.8 g;
In room conditions (temperature 25 ~ 30 DEG C, humidity 30 ~ 70%), above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate;
Fly pupa utilizes the second formulation material to feed its adult after sprouting wings, second formulation material is made up of each component of following percetage by weight: whole milk powder 70.0%, brown sugar 29.8% and nicotine 0.2%, described percentage is percetage by weight, produces after newly hatched larvae until it, enters the acclimatization culture of next generation;
Repeat by above-mentioned steps, the continuous domestication through 30 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain.
embodiment 8
Other operations are with embodiment 7, and form unlike each component of described first formulation material by following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Described second formulation material is made up of each component of following percetage by weight: whole milk powder 70.00%, brown sugar 29.85% and nicotine 0.25%.Continuous domestication through 25 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain.
embodiment 9
Other operations are with embodiment 7, and form unlike each component of described first formulation material by following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Described second formulation material is made up of each component of following percetage by weight: whole milk powder 70.00%, brown sugar 29.7% and nicotine 0.3%.Continuous domestication through 20 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain.
comparative example 1
Operation, with embodiment 1, is made up of each component of following percetage by weight unlike the described collection composts or fertilisers of cultivating that lures: wheat bran 95%, imported fish meal 5%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Experiment finds to utilize and as above lures collection composts or fertilisers of cultivating almost to lure collection to come to produce newly hatched larvae less than Sarcophga fuscicauda kind.
comparative example 2
Operation, with embodiment 1, is made up of each component of following percetage by weight unlike the described collection composts or fertilisers of cultivating that lures: wheat bran 80% and imported fish meal 20%.Experiment find to utilize as above lure collection composts or fertilisers of cultivating lure collection to newly hatched larvae be not the kind of resistance to nicotine.
Embodiment 10
The Sarcophga fuscicauda kind embodiment of the present invention 1 to 3 screened, embodiment 4 to 6 are through cultivating the Sarcophga fuscicauda kind of nicotine formulation material of tolerance 3 ‰, embodiment 7 to 9 through too much carrying out resistance to nicotine aptitude tests for the Sarcophga fuscicauda kind after the Sarcophga fuscicauda kind of continuous domestication cultivation, each comparative example process, and test process is:
Get the newly hatched larvae 0.5g that the Sarcophga fuscicauda adult after described embodiment cultivation process produces respectively, access formulation material (wheat bran 80%, imported fish meal 20% containing the better quality percent concentration nicotine aqueous solution that 1kg is heavy respectively, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 60%) in, test result is shown in Table 1:
Table 1
Test result illustrates, comparative example 1,2 Sarcophga fuscicauda kind can not tolerate the nicotine aqueous solution of mass percent concentration 3.0 ‰.
There occurs diapause phenomenon in the formulation material that embodiment 1 to 3 modulate at the nicotine aqueous solution of mass percent concentration 3.0 ‰ through the Sarcophga fuscicauda kind of screening, larval weight comparatively gently, and has relatively large death.The resistance to nicotine ability of the Sarcophga fuscicauda kind after embodiment 4 to 9 hot housing process improves greatly, can tolerate the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰.
Embodiment 11 stability experiment
(wherein fly maggot culture material formula is wheat bran 80%+ fish meal 20% the resistance to nicotine Sarcophga fuscicauda kind obtained through method described in embodiment 4 to 6 to be carried out routine cultivation, with clear water, above-mentioned formulation material being adjusted to humidity is 60%, and adult forage formula is whole milk powder 70%+ brown sugar 30%) for subsequent use after 20 generations.Simultaneously, the resistance to nicotine Sarcophga fuscicauda strain obtained through method described in embodiment 7 to 9 is utilized to cultivate and expects that (wherein fly maggot culture material formula is wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 60%, and adult forage formula is whole milk powder 70.00%, brown sugar 29.7% and nicotine 0.3%) continue after cultivation 20 generation more for subsequent use.The Sarcophga fuscicauda kind of above-mentioned two strains is carried out resistance to nicotine aptitude tests, detection culture material formula is wheat bran 80%, imported fish meal 20%, the humidity with the nicotine aqueous solution of mass percent concentration 3 ‰ above-mentioned formulation material being adjusted to formulation material is 60%, and whether the resistance to nicotine ability detecting its offspring declines.Test result is shown in Table 2:
The stability experiment result of the Sarcophga fuscicauda strain of table 2 after 30 generations
| Process | Larvae development go through the phase (my god) | Larva number/g(bar) | Survival rate of larvae (%) |
| The conventional strain of embodiment 4 | 7 | 21 | 64 |
| The conventional strain of embodiment 5 | 7 | 20 | 65 |
| The conventional strain of embodiment 6 | 7 | 20 | 66 |
| Embodiment 7 cultivates strain | 5 | 12 | 96 |
| Embodiment 8 cultivates strain | 5 | 12 | 96 |
| Embodiment 9 cultivates strain | 5 | 12 | 96 |
Result shows that the resistance to nicotine Sarcophga fuscicauda kind after strengthening is after cultivating for 20 generations with cellar culture material, resistance to nicotine ability there occurs degradation phenomena, and the stability tolerated nicotine through the resistance to nicotine Sarcophga fuscicauda lines progenies that the present invention cultivates acquisition is fine, and in formulation material compositing range of the present invention, the Sarcophga fuscicauda that the formulation material of the same weight composition of different batches obtains has stable way to cultivate.
Claims (9)
1. a breeding method for resistance to nicotine Sarcophga fuscicauda strain, is characterized in that, comprise the steps:
S1., under indoor conditions, be that the newly hatched larvae of the Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰ is seeded in the first formulation material by tolerance mass percent concentration, Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate;
S2. fly pupa utilizes the second formulation material to feed its adult after sprouting wings, and produces after newly hatched larvae until it, enters the acclimatization culture of next generation;
S3. repeat by above-mentioned steps, the continuous domestication through 20 ~ 30 generations is cultivated, and obtains stable resistance to nicotine Sarcophga fuscicauda strain;
Wherein, described first formulation material is made up of each component of following percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%;
Second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7 ~ 29.8% and nicotine 0.2 ~ 0.3%.
2. breeding method according to claim 1, it is characterized in that, indoor conditions described in S1 is temperature 25 ~ 30 DEG C, humidity 30 ~ 70%.
3. breeding method according to claim 1, it is characterized in that, described first formulation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
4. breeding method according to claim 1, it is characterized in that, described second formulation material is made up of each component of following percetage by weight: whole milk powder 70%, brown sugar 29.7% and nicotine 0.3%.
5. breeding method according to claim 1, is characterized in that, the compound method of described first formulation material is that after each component mixing by the first formulation material, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
6. breeding method according to claim 1, is characterized in that, access Sarcophga fuscicauda newly hatched larvae 0.5 ~ 1.0 g among described every 1 kg first formulation material.
7. breeding method according to claim 1, it is characterized in that, described tolerance mass percent concentration is the preparation method of the resistance to nicotine Sarcophga fuscicauda kind of the nicotine aqueous formulation material of 3 ‰: first from wild Sarcophga fuscicauda kind, filter out resistance to nicotine Sarcophga fuscicauda kind, and then the resistance to nicotine Sarcophga fuscicauda kind screened is carried out hot housing, make it tolerate nicotine aqueous formulation material that mass percent concentration is 3 ‰.
8. breeding method according to claim 7, it is characterized in that, the method for the screening of described resistance to nicotine Sarcophga fuscicauda kind is:
S1. configuration lures collection composts or fertilisers of cultivating, and collection composts or fertilisers of cultivating will be lured to load in open containers;
The described collection composts or fertilisers of cultivating that lures is containing, for example each component of lower percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰ ~ 2 ‰, above-mentioned formulation material being adjusted to humidity is 60%;
S2., when weather is fine, by being equipped with, lure that the container of collection composts or fertilisers of cultivating is put in house refuse heap respectively, chicken house and pig farm lure the wild Sarcophga fuscicauda newly hatched larvae of collection in the morning, regain the container be put in everywhere in the afternoon;
S3. each container reclaimed is placed on indoor cultivation, when waiting the wild Sarcophga fuscicauda newly hatched larvae luring collection to arrive to cultivate aging to larva, utilizes being separated with formulation material by fly maggot from food phase behavior of maggots of boettcherisca peregrine;
S4. by the Morphological Identification of Sarcophga fuscicauda larva, maggots of boettcherisca peregrine is chosen;
S5. filter out 4 ~ 5 days aging, every bar fly maggots and weigh 0.06 ~ 0.08 g, figure is plump, color is golden yellow maggots of boettcherisca peregrine, at ambient temperature, allow maggots of boettcherisca peregrine naturally pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
9. breeding method according to claim 7, it is characterized in that, the method for the hot housing of described resistance to nicotine Sarcophga fuscicauda kind is:
Starting stage: the newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%; In room conditions, above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, nursing adult;
1st strain: after the adult of starting stage produces newly hatched larvae, the newly hatched larvae produced adopts the 1st hot housing material to carry out hot housing; Described 1st hot housing material is made up of each component of following percetage by weight: wheat bran 70 ~ 90%, imported fish meal 10 ~ 30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, S is selected from 0.1 ~ 0.5; In room conditions, above-mentioned Sarcophga fuscicauda newly hatched larvae is cultivated aging to larva, pupate, sprout wings after, nursing adult;
The N+1 time strain: again hot housing is carried out to the newly hatched larvae that the adult of the N time strain gained produces, and component meets following ratio contained by the composts or fertilisers of cultivating that the N+1 time strain is used: the mass percent concentration of the nicotine aqueous solution=content+S of nicotine (in the N time hot housing material) ‰, all the other constituent contents are constant, N be more than or equal to 1 natural number, repeat strengthening, in composts or fertilisers of cultivating described in last strain, the mass percent concentration of the nicotine aqueous solution reaches till 3 ‰.
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