CN104285894B - Strengthening method of nicotine-resistant boettcherisca peregrina variety - Google Patents
Strengthening method of nicotine-resistant boettcherisca peregrina variety Download PDFInfo
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- CN104285894B CN104285894B CN201410421758.2A CN201410421758A CN104285894B CN 104285894 B CN104285894 B CN 104285894B CN 201410421758 A CN201410421758 A CN 201410421758A CN 104285894 B CN104285894 B CN 104285894B
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- nicotine
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- newly hatched
- aqueous solution
- sarcophga fuscicauda
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
The invention discloses a strengthening method of a nicotine-resistant boettcherisca peregrina variety. According to the strengthening method, initial composts are used for breeding screened early larvae laid by a wild nicotine-resistant boettcherisca peregrina variety into imagoes; early larvae laid by the imagoes are subjected to N times of strengthening cultivation into imagoes through strengthening composts; the mass percent concentration of a nicotine water solution is increased by 0.1-0.5 percent points on the basis of last strengthening compost in each strengthening cultivation cycle till the mass percent concentration of the nicotine water solution reaches 0.3%. By means of the strengthening method, the nicotine water solution with the proper concentration is directly adopted for scientific compatibility with bran and imported fish meal, strengthening cultivation of boettcherisca peregrina is successful, the endurance capacity of the wild nicotine-resistant lucilia sericata variety to nicotine can be enhanced step by step, and a more stable cultivation effect is achieved.
Description
Technical field
The present invention relates to animal varietiess triage techniqueses field is and in particular to a kind of strengthening of resistance to nicotine Sarcophga fuscicauda kind
Method.
Background technology
Inapplicable fresh tobacco leaf refers to not be suitable for the fresh tobacco leaves of cured tobacco production（Water content 80～90%）, big with document
Measure the waste/hypo-tobacco leaf producing in the production process of flue-cured tobacco referring to or discarded tobacco leaf（Water content about 15%）Different.Inapplicable Nicotiana tabacum L. leads to
Refer to after cigarette strain normally binds and remove 2～3 milk foot leaves, cigarette industry is inapplicable, need to be under field is removed and processed
Portion's leaf and upper leaf, main include cigarette strain bottom because illumination deficiency, dysplasia, inclusions are not enough, estimated roasting rear inferior quality,
Do not possess 2 inferior leads that baking is worth, and the hacking of cigarette strain top bad, curtailment, structure are stiff, roasting after inferior quality
2 tops.
If these inapplicable Nicotiana tabacum Lves arbitrarily abandon field, easily cause environmental pollution and tobacco viruses repeat feel
Dye, the processing mode destroyed or fill of concentrating according to prior art then needs to spend substantial amounts of expense, and current industry is per ton not
It is suitable for averagely up to 200 yuan of the processing cost of Nicotiana tabacum L., each Nicotiana tabacum L. producing region generally requires under the organization leadership of government of local party committee,
Carry out policy propaganda, tabulation registration, build treatment facility, unified remove, record archive, publicity, cash the measures such as subsidy and supervise
Carry out the process of inapplicable Nicotiana tabacum L., waste time and energy costly.
Using the insecticide of resistance to nicotine, inapplicable Nicotiana tabacum L. being carried out with biological treatment is a kind of preferable alternate process method, ring
Protect, high financial profit.In order to realize, using insecticide, inapplicable fresh tobacco leaf of carrying out a biological disposal upon, need to filter out the insecticide product of resistance to nicotine first
Kind.
The applicant studies for a long period of time based on to housefly and inapplicable fresh tobacco leaf treatment technology, sums up using inapplicable fresh
The method that Nicotiana tabacum L. carries out screening, strengthening and the cultivation of resistance to nicotine strain to housefly.Described strengthening is by the resistance to nicotine man filtering out
The newly hatched larvae of fly is inoculated in inapplicable Nicotiana tabacum L. compost and cultivates to adult, then by the newly hatched larvae of adult output using strengthening training
Nutriment carries out n times hot housing to adult, in each hot housing circulation inapplicable fresh tobacco leaf account for the ratio of compost all can be
Improve 5～15 points, until ratio in hot housing material for the inapplicable fresh tobacco leaf reaches on the basis of last hot housing material
To 85%, the housefly kind that this stage turns out has higher tolerance to nicotine.Described inapplicable Nicotiana tabacum L. compost contain as
Each component of lower percetage by weight：Inapplicable fresh tobacco leaf 30%, wheat bran 60%, imported fish meal 5%, bean cake 5%.
This technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but different batches cultivate the aging fly obtaining
The resistance to nicotine ability of maggot is also otherwise varied, there is the uncertainty of effect.Main reason is that the application aspect of inapplicable Nicotiana tabacum L.,
Although the inapplicable Nicotiana tabacum L. summing up interpolation zone of reasonableness in formulation material through creative research and many experiments can reduce cigarette
The toxic action to Musca domestica larva for the alkali, but affect inapplicable fresh tobacco leaf nicotine content factor very various, complicated mechanism it is more difficult to
Realize accurately controlling.The factor of impact nicotine content in tobacco leaf mainly includes：（1）Different growing stages, in Nicotiana tabacum L., contained nicotine contains
Amount is different：In tobacco seed nicotine content be 0, rise in seedling raising process beginning formed in root, after developing with root system,
The synthesis of nicotine and accumulation ability gradually strengthen, so different growing stages, in Nicotiana tabacum L., contained nicotine content is different；（2）Cigarette strain is not
Same position, nicotine content is dramatically different, and nicotine content is also subject to leaf position and number of sheets large effect；（3）Contained by different cultivars Nicotiana tabacum L.
Nicotine amount is different；（4）Weather conditions of soil dry-wet etc. all have the impact of different savings to nicotine content；（5）Agronomic measures
Different appreciable impact nicotine content of tobacco leaves, including fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and cut root fertilizer, adopt
Nicotine content in debit's formula etc. factor all appreciable impact Nicotiana tabacum Lves, in the nitrogenous fertilizer of three kinds of forms, applies taking fertilization type as a example
After carbamide, nicotine accumulation highest, ammonium nitrate takes second place, and the nicotine accumulation that potassium nitrate is processed is minimum, and than applying, carbamide is low by 27%；To pinch
As a example period, the research such as Zhang Dan finds, postpones nicotine accumulation intensity peak with Physical property and declines successively, nicotine content is also successively
Lower, often postpone and pinching for one day, nicotine content just declines 0.0504%；Again taking cut root fertilizer as a example, cut root 1/8 and do not cut root phase
Upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33% to ratio, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
How to avoid using different batches compost cultivate obtain aging fly larvae effect uncertainty be this area urgently
Technical barrier to be solved.And cultivate the more insect strain of resistance to nicotine of multi items and carry out using stablizing feasible technical scheme
Reinforced cultivating effectively is this area simultaneously need to the important technological problems that solve.
Content of the invention
The technical problem to be solved in the present invention is to lack strong resistance to of the tolerance to nicotine in prior art to overcome
The defect of nicotine Sarcophga fuscicauda kind, provides a kind of intensifying method of resistance to nicotine Sarcophga fuscicauda kind.
The purpose of the present invention is achieved by the following technical programs：
Sarcophga fuscicauda is another kind of insecticide different from housefly, and it is a kind of important economic insects and scavenger insect,
Available Sarcophga fuscicauda larva processes various corruption organic wastes, such as beer residue, bean dregs, chicken manure, pig manure and changing food waste
Deng.Existing Sarcophga fuscicauda kind all has preferable biological treatment capabilities to nontoxic organic waste, but to inapplicable fresh tobacco leaf
Treatment effect not good.Main cause is because containing the toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, to brown tail
Other sarcophagid larva（Maggots of boettcherisca peregrine）There is certain murder by poisoning and the effect of Developing restraint.In order to biological using Sarcophga fuscicauda
Process inapplicable fresh tobacco leaf, the Sarcophga fuscicauda kind of resistance to nicotine need to be filtered out first from wild Sarcophga fuscicauda kind, improve
The tolerance to fresh tobacco leaf for the Sarcophga fuscicauda.
The present invention provides a kind of intensifying method of resistance to nicotine Sarcophga fuscicauda kind, comprises the steps：
Starting stage：The newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described initial
Compost contains each component of following percetage by weight：Wheat bran 70～90%, imported fish meal 10～30%, use mass percent concentration
It is 60% that above-mentioned formulation material is adjusted to humidity by 1 ‰ nicotine aqueous solution；In room conditions, by above-mentioned fly newly hatched larvae cultivate to
Larva is aging, pupate, sprout wings after, feed its adult using milk powder, brown sugar and water；
1st strain：After the adult of starting stage produces newly hatched larvae, the newly hatched larvae being produced adopts the 1st
Secondary hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight：Wheat bran
80%th, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is
60%, S are selected from 0.1～0.5；In room conditions, by above-mentioned fly newly hatched larvae cultivate to larva aging, pupate, sprout wings after, feed
Support its adult；
The N+1 time strain：The newly hatched larvae that the adult of n-th strain gained is produced carries out strengthening training again
Support, and component contained by the compost used by the N+1 time strain meets following ratio：The mass percent of nicotine aqueous solution
Concentration=（Content+the S of nicotine in n-th hot housing material）‰, remaining constituent content is constant, and N is the natural number more than or equal to 1,
Repeat to strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
The Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰,
1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、2.3‰、2.4‰、
2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences 20 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰,
1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences altogether
10 hot housing.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰,
2.5‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences 4 hot housing altogether.
Described resistance to nicotine Sarcophga fuscicauda kind refers to the resistance to nicotine palm fibre tail filtering out from wild Sarcophga fuscicauda kind
Other sarcophagid kind.
Described filter out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind concrete grammar be：
S1. configuration traps compost, will trap compost and load in open containers；
Described trap each component that compost contains following percetage by weight：Wheat bran 70～90%, imported fish meal 10～30%,
With the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 60%；
S2. when weather is fine, the morning 8:00 container that will be equipped with trapping compost be put in respectively house refuse heap, chicken house and
Pig farm is trapping wild Sarcophga fuscicauda newly hatched larvae, afternoon 17:00 withdraws the container being put in everywhere；
S3. by reclaim each container be placed on indoor cultivation, wait the wild Sarcophga fuscicauda newly hatched larvae trapping cultivate to
When larva is aging, it is separated with formulation material from food phase behavior using maggots of boettcherisca peregrine（When i.e. maggots of boettcherisca peregrine is aging
Can voluntarily climb from compost, find dry place and pupate）；
S4. pass through the Morphological Identification of Sarcophga fuscicauda larva, choose maggots of boettcherisca peregrine；
S5. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow maggots of boettcherisca peregrine naturally to pupate, sprout wings after can obtain the Sarcophga fuscicauda of resistance to nicotine
Kind.
Preferably, described indoor conditionss are 25～30 DEG C of temperature, humidity 30～70%.
The collocation method of described initial incubation material is by after each component mixing of initial incubation material, uses mass percent concentration
It is 60% that above-mentioned formulation material is adjusted to humidity by 1 ‰ nicotine aqueous solution.
Preferably, access Sarcophga fuscicauda newly hatched larvae 0.5～1.0 g in every 1 kg initial incubation material；Every 1 kg strengthening
Sarcophga fuscicauda newly hatched larvae 0.5～1.0 g is accessed in compost.
Described its adult of nursing is all to be fed using whole milk powder, brown sugar and water.Preferably, according to percent by weight,
Described whole milk powder, the usage ratio of brown sugar are whole milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with using sponge
Water, sucks for adult, can be using the tap water of cleaning or pure water.
The invention has the beneficial effects as follows：
Proportion compatibility is determined by science, the present invention creatively directly adopts nicotine aqueous solution and the wheat bran of suitable concentration
With imported fish meal scientific compatibility, the first formulation material of acquisition not only successfully covers the unpleasant of nicotine and bitterness, nutritional labeling suitably,
Ensure Sarcophga fuscicauda larval growth smoothly, and because the appropriateness of nicotine content and definitiveness, and initial incubation material and
Before and after nutrient substance contained by hot housing material and nicotine equal size, the reasonability of configuration is it is ensured that in same nurturing an environment, no
Formulation material with the same weight composition of batch obtains stable cultivate effect.
By the hot housing method of the present invention, both can effectively step up the tolerance energy to nicotine for the Sarcophga fuscicauda kind
Power, is unlikely to cause serious diapause to Sarcophga fuscicauda kind again, can strengthen wild Sarcophga fuscicauda kind pair by easy stages
The tolerance of nicotine, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost.
Specific embodiment
Further describe the present invention with reference to specific embodiment.Unless stated otherwise, the present invention adopt reagent,
Equipment is the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
Embodiment 1 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition：Above-mentioned formulation material is adjusted to humidity with the nicotine aqueous solution of mass percent concentration 1 ‰ by wheat bran 90%, imported fish meal 10%
For 60%.
S02. by the above-mentioned compost modulating, every 1 kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, by the wild Sarcophga fuscicauda newly hatched larvae training trapping
Educate aging to larva, from food phase behavior, fly larvae is separated with formulation material using maggots of boettcherisca peregrine（I.e. maggots of boettcherisca peregrine is old
Can voluntarily climb when ripe from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, exclude it
His fly kind.
S05. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally to pupate, sprout wings after can obtain resistance to nicotine brown tail not numb
Fly kind.
Embodiment 2 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition：Above-mentioned formulation material is adjusted to wet by wheat bran 80%, imported fish meal 20% with the nicotine aqueous solution of mass percent concentration 1.5 ‰
Spend for 60%.
S02. by the above-mentioned compost modulating, every 1kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, by the wild Sarcophga fuscicauda newly hatched larvae training trapping
Educate aging to larva, from food phase behavior, fly larvae is separated with formulation material using maggots of boettcherisca peregrine（I.e. maggots of boettcherisca peregrine is old
Can voluntarily climb when ripe from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, exclude it
His fly kind.
S05. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally to pupate, sprout wings after can obtain resistance to nicotine brown tail not numb
Fly kind.
Embodiment 3 filters out resistance to nicotine Sarcophga fuscicauda kind from wild Sarcophga fuscicauda kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition：Above-mentioned formulation material is adjusted to humidity with the nicotine aqueous solution of mass percent concentration 2 ‰ by wheat bran 70%, imported fish meal 30%
For 60%.
S02. by the above-mentioned compost modulating, every 1 kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, by the wild Sarcophga fuscicauda newly hatched larvae training trapping
Educate aging to larva, from food phase behavior, fly larvae is separated with formulation material using maggots of boettcherisca peregrine（I.e. maggots of boettcherisca peregrine is old
Can voluntarily climb when ripe from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, individually choose maggots of boettcherisca peregrine with tweezers, exclude it
His fly kind.
S5. 4～5 days brown tails aging, that every fly larvae weighs 0.06～0.08 g, figure is plump, color is golden yellow are filtered out other
Sarcophagid maggot, at ambient temperature, allow these maggots of boettcherisca peregrine naturally to pupate, sprout wings after can obtain resistance to nicotine brown tail not numb
Fly kind.
Embodiment 4 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screening makes it tolerate percent mass
Specific concentration is the formulation material of 3 ‰ nicotine aqueous solution
The resistance to nicotine Sarcophga fuscicauda kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated
Mass percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows：
S11. the starting stage：
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 90%, enter
Mouth fish flour 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*12cm）, it is not numb that every basin accesses brown tail
Fly newly hatched larvae 0.8 g；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, by above-mentioned fly newly hatched larvae cultivate to
Larva is aging, pupate, sprout wings after, feed its adult.Using the feed mixtures of milk powder and brown sugar, the present embodiment is according to weight hundred
Divide than meter, milk powder 70%, brown sugar 30%.Separately it is saturated with water with sponge, take food for it（Similarly hereinafter）.
Strain：
S12. the 1st strain：After the adult of starting stage produces newly hatched larvae, the newly hatched larvae being produced adopts
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight：Bran
Skin 80%, imported fish meal 20%, use mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity and is by ‰ nicotine aqueous solution
60%, S are selected from 0.1～0.5；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, above-mentioned fly newly hatched larvae is cultivated
To larva aging, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain：The newly hatched larvae that the adult of n-th strain gained is produced is strengthened again
Culture, and component contained by the compost used by the N+1 time strain meets following ratio：The percent mass of nicotine aqueous solution
Specific concentration=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats to strengthen, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.1‰、1.2‰、1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、
2.3‰、2.4‰、2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences altogether
20 hot housing.The Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
Embodiment 5 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screening makes it tolerate percent mass
Specific concentration is the formulation material of 3 ‰ nicotine aqueous solution
The resistance to nicotine Sarcophga fuscicauda kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated
Mass percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows：
S11. the starting stage：
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 80%, enter
Mouth fish flour 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*12cm）, it is not numb that every basin accesses brown tail
Fly newly hatched larvae 0.8 g；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, by above-mentioned fly newly hatched larvae cultivate to
Larva is aging, pupate, sprout wings after, feed its adult.
Strain：
S12. the 1st strain：After the adult of starting stage produces newly hatched larvae, the newly hatched larvae being produced adopts
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight：Bran
Skin 80%, imported fish meal 20%, use mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity and is by ‰ nicotine aqueous solution
60%, S are selected from 0.1～0.5；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, above-mentioned fly newly hatched larvae is cultivated
To larva aging, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain：The newly hatched larvae that the adult of n-th strain gained is produced is strengthened again
Culture, and component contained by the compost used by the N+1 time strain meets following ratio：The percent mass of nicotine aqueous solution
Specific concentration=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats to strengthen, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.2‰、1.4‰、1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely at the beginning of Sarcophga fuscicauda
Incubate larva and experience 10 hot housing altogether.The Sarcophga fuscicauda kind that this stage turns out has higher tolerance to nicotine.
Embodiment 6 carries out hot housing to the resistance to nicotine Sarcophga fuscicauda kind screening makes it tolerate percent mass
Specific concentration is the formulation material of 3 ‰ nicotine aqueous solution
The resistance to nicotine Sarcophga fuscicauda kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated
Mass percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows：
S11. the starting stage：
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 70%, enter
Mouth fish flour 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin（Long * width * height=45cm*30cm*12cm）, it is not numb that every basin accesses brown tail
Fly newly hatched larvae 0.8 g；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, by above-mentioned fly newly hatched larvae cultivate to
Larva is aging, pupate, sprout wings after, feed its adult.
Strain：
S12. the 1st strain：After the adult of starting stage produces newly hatched larvae, the newly hatched larvae being produced adopts
1st time hot housing material carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight：Bran
Skin 80%, imported fish meal 20%, use mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity and is by ‰ nicotine aqueous solution
60%, S are selected from 0.1～0.5；In room conditions（25～30 DEG C of temperature, humidity 30～70%）, above-mentioned fly newly hatched larvae is cultivated
To larva aging, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain：The newly hatched larvae that the adult of n-th strain gained is produced is strengthened again
Culture, and component contained by the compost used by the N+1 time strain meets following ratio：The percent mass of nicotine aqueous solution
Specific concentration=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the nature more than or equal to 1
Number, repeats to strengthen, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ and is in compost described in last strain
Only.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.5‰、2.0‰、2.5‰、3.0‰；Namely Sarcophga fuscicauda newly hatched larvae experiences 4 hot housing altogether.This stage turns out
Sarcophga fuscicauda kind there is higher tolerance to nicotine.
Comparative example 1
Operation is grouped into by each group of following percetage by weight with embodiment 1, the except for the difference that described compost that traps：Wheat bran
95%th, imported fish meal 5%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Experiment
Find using as above trapping compost and almost trap to come to produce newly hatched larvae less than Sarcophga fuscicauda kind.
Comparative example 2
Operation is grouped into by each group of following percetage by weight with embodiment 1, the except for the difference that described compost that traps：Wheat bran
80% and imported fish meal 20%.Experiment finds using as above trapping the product that the newly hatched larvae that compost traps is not resistance to nicotine
Kind.
Embodiment 7
The Sarcophga fuscicauda kind that the embodiment of the present invention 1 to 3 is screened, embodiment 4 to 6 tolerate 3 ‰ through culture
The Sarcophga fuscicauda kind of nicotine formulation material, the Sarcophga fuscicauda kind after each comparative example is processed carries out resistance to nicotine ability and surveys
Try, test process is：
The newly hatched larvae 0.5g that Sarcophga fuscicauda adult after taking described embodiment culture to process respectively is produced, is respectively connected to
The formulation material of the nicotine aqueous solution of percent concentration containing better quality of 1kg weight（Wheat bran 80%, imported fish meal 20%, use percent mass
It is 60% that above-mentioned formulation material is adjusted to humidity by the nicotine aqueous solution of specific concentration 3.0 ‰）In, test result is shown in Table 1：
Table 1
| Process | Larvae development goes through the phase（My god） | Larva bar number/g（Bar） | Survival rate of larvae（%） |
| Embodiment 1 is initially planted | 7 | 23 | 61 |
| Embodiment 2 is initially planted | 7 | 20 | 67 |
| Embodiment 3 is initially planted | 7 | 25 | 64 |
| Comparative example 1 | —— | —— | —— |
| Comparative example 2 | 8 | 34 | 14 |
| Embodiment semi-finals kind | 5 | 13 | 96 |
| Embodiment 5 strengthening kind | 5 | 14 | 95 |
| Embodiment 6 strengthening kind | 5 | 16 | 93 |
Test result illustrates, comparative example 1,2 Sarcophga fuscicauda kinds are not resistant to the nicotine water of mass percent concentration 3.0 ‰
Solution.
1 to 3 Sarcophga fuscicauda kind through screening of embodiment is adjusted in the nicotine aqueous solution of mass percent concentration 3.0 ‰
Diapause phenomenon is there occurs, larval weight is lighter, and has relatively large death in the formulation material of system.Through this after embodiment 4 to 6 screening
The resistance to nicotine ability of the Sarcophga fuscicauda kind after bright method hot housing process greatly improves, and can tolerate mass percent dense
Spend the formulation material of 3.0 ‰ nicotine aqueous solution modulation.
Claims (6)
1. a kind of intensifying method of resistance to nicotine Sarcophga fuscicauda kind is it is characterised in that comprise the steps：
Starting stage：The newly hatched larvae of resistance to nicotine Sarcophga fuscicauda kind is seeded in initial incubation material, described initial incubation
Material is grouped into by the group of following weight fraction：Wheat bran 70～90%, imported fish meal 10～30%, use mass percent concentration
It is 60% that described initial incubation material is adjusted to humidity by 1 ‰ nicotine aqueous solution；In room conditions, above-mentioned newly hatched larvae is cultivated
To larva aging, pupate, sprout wings after, feed its adult；
1st strain：After the adult of starting stage produces newly hatched larvae, the newly hatched larvae being produced adopts the 1st strengthening
Compost carries out hot housing；Described 1st hot housing material is grouped into by each group of following weight fraction：Wheat bran 70～
90%th, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰ by described 1st hot housing
It is that 60%, S is selected from 0.1～0.5 that material is adjusted to humidity；In room conditions, by above-mentioned newly hatched larvae cultivate to larva aging, change
After pupa, emergence, feed its adult；
The N+1 time strain：The newly hatched larvae that the adult of n-th strain gained is produced carries out hot housing again, and
And component contained by the compost used by the N+1 time strain meets following ratio：The mass percent concentration of nicotine aqueous solution
=(content+S of nicotine in n-th hot housing material) ‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, weight
Multiple strengthening, till in the compost used by until last strain, the mass percent concentration of nicotine aqueous solution reaches 3 ‰；
The screening technique of described resistance to nicotine Sarcophga fuscicauda kind is：
S1. configuration traps compost, will trap compost and load in open containers；
The described compost that traps is grouped into by each group of following weight fraction：Wheat bran 70～90%, imported fish meal 10～30%,
Trap compost to be adjusted to humidity be 60% by described with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰；
S2., when weather is fine, the container that will be equipped with trapping compost in the morning is put in house refuse heap, chicken house and pig farm to lure respectively
Collect wild Sarcophga fuscicauda newly hatched larvae, withdraw the container being put in everywhere in the afternoon；
S3. each container reclaiming is placed on indoor cultivation, waits the wild Sarcophga fuscicauda newly hatched larvae trapping to cultivate to larva
When aging, fly larvae is separated with trapping compost from food phase behavior using maggots of boettcherisca peregrine；
S4. pass through the Morphological Identification of Sarcophga fuscicauda larva, choose maggots of boettcherisca peregrine；
S5. 4～5 days Sarcophga fuscicaudas aging, that every fly larvae weighs 0.06～0.08g, figure is plump, color is golden yellow are filtered out
Maggot, at ambient temperature, allow maggots of boettcherisca peregrine naturally to pupate, sprout wings after can obtain the Sarcophga fuscicauda kind of resistance to nicotine.
2. according to claim 1 intensifying method it is characterised in that described indoor conditionss be 25～30 DEG C of temperature, humidity 30
～70%.
3. according to claim 1 intensifying method it is characterised in that the collocation method of described initial incubation material is to train initial
After each component of nutriment mixes, add the nicotine aqueous solution of certain concentration that the humidity of initial incubation material is adjusted to 60%.
4. according to claim 1 intensifying method it is characterised in that accessing at the beginning of Sarcophga fuscicauda in every 1kg initial incubation material
Incubate larva 0.5～1.0g；Sarcophga fuscicauda newly hatched larvae 0.5～1.0g is accessed in every 1kg hot housing material.
5. according to claim 1 intensifying method it is characterised in that its adult of described nursing is all using whole milk powder, red
Sugar and water are fed.
6. according to claim 5 intensifying method it is characterised in that according to percent by weight, described whole milk powder, brown sugar
Usage ratio be whole milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with water using sponge, sucks for adult.
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