CN104285899B - Method for strengthening nicotine-resisting chrysomyia megacephala breeds - Google Patents
Method for strengthening nicotine-resisting chrysomyia megacephala breeds Download PDFInfo
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- CN104285899B CN104285899B CN201410421797.2A CN201410421797A CN104285899B CN 104285899 B CN104285899 B CN 104285899B CN 201410421797 A CN201410421797 A CN 201410421797A CN 104285899 B CN104285899 B CN 104285899B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
- A23K10/28—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin from waste dairy products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Abstract
The invention discloses a method for strengthening nicotine-resisting chrysomyia megacephala breeds. The method comprises the steps that spawns produced by the screened wild nicotine-resisting chrysomyia megacephala breeds are bred into adults through initial breeding materials with nicotine, and the spawns produced by the adults are strengthened N times through proper strengthening breeding materials and bred into adults. The initial breeding materials are composed of, by weight, 70 percent to 85 percent of bran and 15 percent to 30 percent of imported fish meal, and the humidity of the formula materials is adjusted to 60 percent through the nicotine aqueous solution with the mass percent concentration of one permillage. The nicotine aqueous solution with the proper concentration is directly and scientifically combined with the bran and the imported fish meal, chrysomyia megacephala is successfully strengthened and bred, the nicotine resisting capacity of the chrysomyia megacephala can be effectively and gradually improved, severe dispause of the chrysomyia megacephala cannot be caused, the nicotine resisting capacity of the wild chrysomyia megacephala breeds can be strengthened step by step, and the more stable breeding effect is obtained.
Description
Technical field
The present invention relates to animal varietiess triage techniqueses field is and in particular to a kind of strengthening side of the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind
Method.
Background technology
Inapplicable fresh tobacco leaf refers to not be suitable for the fresh tobacco leaves (water content 80～90%) of cured tobacco production, big with document
Measure the waste/hypo-tobacco leaf producing in the production process of flue-cured tobacco referring to or discarded tobacco leaf (water content about 15%) is different.Inapplicable Nicotiana tabacum L. leads to
Refer to after cigarette strain normally binds and remove 2～3 milk foot leaves, cigarette industry is inapplicable, need to be under field is removed and processed
Portion's leaf and upper leaf, main include cigarette strain bottom because illumination deficiency, dysplasia, inclusions are not enough, estimated roasting rear inferior quality,
Do not possess 2 inferior leads that baking is worth, and the hacking of cigarette strain top bad, curtailment, structure are stiff, roasting after inferior quality
2 tops.
If these inapplicable Nicotiana tabacum Lves arbitrarily abandon field, easily cause environmental pollution and tobacco viruses repeat feel
Dye, the processing mode destroyed or fill of concentrating according to prior art then needs to spend substantial amounts of expense, and current industry is per ton not
It is suitable for averagely up to 200 yuan of the processing cost of Nicotiana tabacum L., each Nicotiana tabacum L. producing region generally requires under the organization leadership of government of local party committee,
Carry out policy propaganda, tabulation registration, build treatment facility, unified remove, record archive, publicity, cash the measures such as subsidy and supervise
Carry out the process of inapplicable Nicotiana tabacum L., waste time and energy costly.
Using the insecticide of resistance to nicotine, inapplicable Nicotiana tabacum L. being carried out with biological treatment is a kind of preferable alternate process method, ring
Protect, high financial profit.In order to realize, using insecticide, inapplicable fresh tobacco leaf of carrying out a biological disposal upon, need to filter out the insecticide product of resistance to nicotine first
Kind.
The applicant studies for a long period of time based on to housefly and inapplicable fresh tobacco leaf treatment technology, sums up using inapplicable fresh
The method that Nicotiana tabacum L. carries out screening, strengthening and the cultivation of resistance to nicotine strain to housefly.Described strengthening is by the resistance to nicotine man filtering out
The ovum of fly is inoculated in inapplicable fresh tobacco leaf compost and cultivates to adult, then the ovum of adult output is utilized inapplicable fresh tobacco leaf strengthening
Compost carries out n hot housing to adult, and in each hot housing circulation, inapplicable fresh tobacco leaf accounts for the ratio of compost and all can
Improve 5～15 points on the basis of upper once hot housing material, until ratio in hot housing material for the inapplicable fresh tobacco leaf
Reach 85%, the housefly kind that this stage turns out has higher tolerance to nicotine.Described inapplicable fresh tobacco leaf compost contains
There is each component of following percetage by weight: inapplicable fresh tobacco leaf 30%, wheat bran 60%, imported fish meal 5%, bean cake 5%.
This technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but different batches cultivate the aging fly obtaining
The resistance to nicotine ability of maggot is also otherwise varied, there is the uncertainty of effect.Main reason is that the application aspect of inapplicable Nicotiana tabacum L.,
Although the inapplicable Nicotiana tabacum L. summing up interpolation zone of reasonableness in formulation material through creative research and many experiments can reduce cigarette
The toxic action to Musca domestica larva for the alkali, but affect inapplicable fresh tobacco leaf nicotine content factor very various, complicated mechanism it is more difficult to
Realize accurately controlling.The factor of impact nicotine content in tobacco leaf specifically includes that (1) different growing stages, and in Nicotiana tabacum L., contained nicotine contains
Amount is different: in tobacco seed, nicotine content is 0, plays beginning and formed in root in seedling raising process, after developing with root system,
The synthesis of nicotine and accumulation ability gradually strengthen, so different growing stages, in Nicotiana tabacum L., contained nicotine content is different；(2) cigarette strain is not
Same position, nicotine content is dramatically different, and nicotine content is also subject to leaf position and number of sheets large effect；(3) contained by different cultivars Nicotiana tabacum L.
Nicotine amount is different；(4) weather conditions of soil dry-wet etc. all have the impact of different savings to nicotine content；(5) agronomic measures
Different appreciable impact nicotine content of tobacco leaves, including fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and cut root fertilizer, adopt
Nicotine content in debit's formula etc. factor all appreciable impact Nicotiana tabacum Lves, in the nitrogenous fertilizer of three kinds of forms, applies taking fertilization type as a example
After carbamide, nicotine accumulation highest, ammonium nitrate takes second place, and the nicotine accumulation that potassium nitrate is processed is minimum, and than applying, carbamide is low by 27%；To pinch
As a example period, the research such as Zhang Dan finds, postpones nicotine accumulation intensity peak with Physical property and declines successively, nicotine content is also successively
Lower, often postpone and pinching for one day, nicotine content just declines 0.0504%；Again taking cut root fertilizer as a example, cut root 1/8 and do not cut root phase
Upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33% to ratio, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
How to avoid using different batches compost cultivate obtain aging fly larvae effect uncertainty be this area urgently
Technical barrier to be solved.And cultivate the more insect strain of resistance to nicotine of multi items and carry out using stablizing feasible technical scheme
Reinforced cultivating effectively is this area simultaneously need to the important technological problems that solve.
Content of the invention
The technical problem to be solved in the present invention is to lack strong resistance to of the tolerance to nicotine in prior art to overcome
The defect of nicotine chrysomyia megacephala (big head golden fly) kind, provides a kind of intensifying method of the chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind of effect stability.
The purpose of the present invention is achieved by the following technical programs:
Chrysomyia megacephala (big head golden fly) is another kind of insecticide different from housefly, and it is a kind of important economic insects and scavenger insect, can
Process various corruption organic wastes, such as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc. using chrysomyia megacephala (big head golden fly) larva.Existing
Chrysomyia megacephala (big head golden fly) kind is had all to have preferable biological treatment capabilities to nontoxic organic waste, but the process effect to inapplicable fresh tobacco leaf
Fruit is not good.Main cause is because containing the toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, to chrysomyia megacephala (big head golden fly) larva
(Chrysomya megacephala maggot) has certain murder by poisoning and the effect of Developing restraint.In order to using the chrysomyia megacephala (big head golden fly) inapplicable fresh cigarette of biological treatment
Leaf, need to filter out the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine first from wild chrysomyia megacephala (big head golden fly) kind, improve chrysomyia megacephala (big head golden fly) to fresh tobacco leaf
Tolerance.
The present invention provides a kind of intensifying method of the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind, comprises the steps:
Starting stage: the ovum of the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material contains
There is each component of following percetage by weight: wheat bran 70～85%, imported fish meal 15～30%, with the cigarette of mass percent concentration 1 ‰
It is 60% that above-mentioned formulation material is adjusted to humidity by aqueous alkali；In room conditions, by above-mentioned fly blow cultivate to larva aging, pupate,
After emergence, feed its adult using milk powder, brown sugar and water；
1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st hot housing material
Carry out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, imported fish meal
20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰ by above-mentioned formulation material be adjusted to humidity be 60%, s be selected from 0.1～
0.5；In room conditions, by above-mentioned fly blow cultivate to larva aging, pupate, sprout wings after, feed its adult；
(n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained, and
Component contained by compost used by (n+1)th strain meets following ratio: the mass percent concentration of nicotine aqueous solution=
(content+s of nicotine in n-th hot housing material) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, repeats
Strengthening, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.This rank
The chrysomyia megacephala (big head golden fly) kind that section is turned out has higher tolerance to nicotine.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰,
1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、2.3‰、2.4‰、
2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum experiences 20 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰,
1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum experiences 10 strengthenings altogether
Culture.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰,
2.5‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum experiences 4 hot housing altogether.
The described chrysomyia megacephala (big head golden fly) of resistance to nicotine kind refers to the chrysomyia megacephala (big head golden fly) of resistance to nicotine filtering out from wild chrysomyia megacephala (big head golden fly) kind
Kind.
Described from wild chrysomyia megacephala (big head golden fly) kind, filter out the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind method particularly includes:
S1. configuration traps compost, will trap compost and load in open containers；
Described trap each component that compost contains following percetage by weight: wheat bran 70～85%, imported fish meal 15～30%,
With the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 60%；
S2. when weather is fine, the morning (preferably 8:00) will be equipped with trapping compost container be put in respectively house refuse heap,
Trapping wild fly blow, withdraw the container being put in everywhere in the afternoon (preferably 17:00) for chicken house and pig farm；
S3. by reclaim each container be placed on indoor cultivation, wait the wild fly blow trapping cultivate to larva aging when, utilize
Being separated it with formulation material from food phase behavior of Chrysomya megacephala maggot (can voluntarily be climbed from compost when i.e. Chrysomya megacephala maggot is aging, seek
Dry place is looked for pupate)；
S4. pass through the Morphological Identification of chrysomyia megacephala (big head golden fly) larva, choose Chrysomya megacephala maggot；
S5. 4～5 days chrysomyia megacephala (big head golden fly)s aging, that every fly larvae weighs 0.05～0.07g, figure is plump, color is golden yellow are filtered out
Maggot, at ambient temperature, allow Chrysomya megacephala maggot naturally to pupate, sprout wings after can obtain the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine.
Preferably, described indoor conditionss are 25～30 DEG C of temperature, humidity 30～70%.
The collocation method of described initial incubation material is by after each component mixing of initial incubation material, uses mass percent concentration
It is 60% that above-mentioned formulation material is adjusted to humidity by 1 ‰ nicotine aqueous solution.
Preferably, access chrysomyia megacephala (big head golden fly) ovum 0.5～1.0g in every 1 kg initial incubation material；Connect in every 1kg hot housing material
Enter chrysomyia megacephala (big head golden fly) ovum 0.5～1.0g.
Described its adult of nursing is all to be fed using whole milk powder, brown sugar and water.Preferably, according to percent by weight,
Described whole milk powder, the usage ratio of brown sugar are whole milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with using sponge
Water, sucks for adult, can be using the tap water of cleaning or pure water.
The invention has the beneficial effects as follows:
Proportion compatibility is determined by science, the present invention creatively directly adopts nicotine aqueous solution and the wheat bran of suitable concentration
With imported fish meal scientific compatibility, the first formulation material of acquisition not only successfully covers the unpleasant of nicotine and bitterness, nutritional labeling suitably,
Ensure chrysomyia megacephala (big head golden fly) larval growth smoothly, and because the appropriateness of nicotine content and definitiveness, and initial incubation material and strong
Change the reasonability of configuration before and after nutrient substance and nicotine equal size contained by compost it is ensured that in same nurturing an environment, different
The formulation material of the same weight composition of batch obtains stable cultivate effect.
By the hot housing method of the present invention, both can effectively step up the tolerance energy to nicotine for the chrysomyia megacephala (big head golden fly) kind
Power, is unlikely to cause serious diapause to chrysomyia megacephala (big head golden fly) kind again, can strengthen wild chrysomyia megacephala (big head golden fly) kind by easy stages to nicotine
Tolerance, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost.
Specific embodiment
Further describe the present invention with reference to specific embodiment.Unless stated otherwise, the present invention adopt reagent,
Equipment is the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
Embodiment 1 filters out the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind from wild chrysomyia megacephala (big head golden fly) kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material is adjusted to humidity
For 60%.
S02. by the above-mentioned compost modulating, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild fly blow trapping is cultivated aging to larva,
Being separated fly larvae with formulation material from food phase behavior using Chrysomya megacephala maggot (can voluntarily climb from culture when i.e. Chrysomya megacephala maggot is aging
Material, finds dry place and pupates).
S04. by the Morphological Identification of larva under anatomical lens, individually choose Chrysomya megacephala maggot with tweezers, exclude other
Fly kind.
S05. 4～5 days major part gold aging, that every fly larvae weighs 0.05～0.07g, figure is plump, color is golden yellow are filtered out
Fly larvae, at ambient temperature, allow these Chrysomya megacephala maggot naturally to pupate, sprout wings after can obtain the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine.
Embodiment 2 filters out the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind from wild chrysomyia megacephala (big head golden fly) kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material is adjusted to humidity
For 60%.
S02. by the above-mentioned compost modulating, every 1kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild fly blow trapping is cultivated aging to larva,
Being separated fly larvae with formulation material from food phase behavior using Chrysomya megacephala maggot (can voluntarily climb from culture when i.e. Chrysomya megacephala maggot is aging
Material, finds dry place and pupates).
S04. by the Morphological Identification of larva under anatomical lens, individually choose Chrysomya megacephala maggot with tweezers, exclude other
Fly kind.
S05. 4～5 days major part gold aging, that every fly larvae weighs 0.05～0.07g, figure is plump, color is golden yellow are filtered out
Fly larvae, at ambient temperature, allow these Chrysomya megacephala maggot naturally to pupate, sprout wings after can obtain the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine.
Embodiment 3 filters out the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind from wild chrysomyia megacephala (big head golden fly) kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 85%, imported fish meal 15%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material is adjusted to wet
Spend for 60%.
S02. by the above-mentioned compost modulating, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild fly blow trapping is cultivated aging to larva,
Being separated fly larvae with formulation material from food phase behavior using Chrysomya megacephala maggot (can voluntarily climb from culture when i.e. Chrysomya megacephala maggot is aging
Material, finds dry place and pupates).
S04. by the Morphological Identification of larva under anatomical lens, individually choose Chrysomya megacephala maggot with tweezers, exclude other
Fly kind.
S5. 4～5 days chrysomyia megacephala (big head golden fly)s aging, that every fly larvae weighs 0.05～0.07g, figure is plump, color is golden yellow are filtered out
Maggot, at ambient temperature, allow these Chrysomya megacephala maggot naturally to pupate, sprout wings after can obtain the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine.
Embodiment 4 carries out hot housing to the chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind screening makes it tolerate mass percent
Concentration is the formulation material of 3 ‰ nicotine aqueous solution
The chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated matter
Amount percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 80%, enter
Mouth fish flour 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and every basin accesses chrysomyia megacephala (big head golden fly)
Ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate,
After emergence, feed its adult (feeding method: according to percent by weight, described whole milk powder, the usage ratio of brown sugar are full-cream
Milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with water using sponge, sucks for adult.Similarly hereinafter).
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, s is selected from 0.1
～0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate, plumage
After change, feed its adult.
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.1‰、1.2‰、1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、
2.3‰、2.4‰、2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum experiences 20 strengthenings altogether
Culture.The chrysomyia megacephala (big head golden fly) kind that this stage turns out has higher tolerance to nicotine.
Embodiment 5 carries out hot housing to the chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind screening makes it tolerate mass percent
Concentration is the formulation material of 3 ‰ nicotine aqueous solution
The chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated matter
Amount percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 70%, enter
Mouth fish flour 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and every basin accesses chrysomyia megacephala (big head golden fly)
Ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate,
After emergence, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, s is selected from 0.1
～0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate, plumage
After change, feed its adult；
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.2‰、1.4‰、1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum is altogether
10 hot housing of experience.The chrysomyia megacephala (big head golden fly) kind that this stage turns out has higher tolerance to nicotine.
Embodiment 6 carries out hot housing to the chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind screening makes it tolerate mass percent
Concentration is the formulation material of 3 ‰ nicotine aqueous solution
The chrysomyia megacephala (big head golden fly) of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated so as to be tolerated matter
Amount percent concentration is the formulation material of 3 ‰ nicotine aqueous solution, and concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 80%, enter
Mouth fish flour 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and every basin accesses chrysomyia megacephala (big head golden fly)
Ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate,
After emergence, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material being adjusted to humidity is that 60%, s is selected from 0.1
～0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned fly blow cultivate to larva aging, pupate, plumage
After change, feed its adult；
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.5‰、2.0‰、2.5‰、3.0‰；Namely chrysomyia megacephala (big head golden fly) ovum experiences 4 hot housing altogether.The major part gold that this stage turns out
Fly kind has higher tolerance to nicotine.
Comparative example 1
Operation is grouped into by each group of following percetage by weight with embodiment 1, the except for the difference that described compost that traps: wheat bran
95%th, imported fish meal 5%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Experiment
Find using as above trap compost almost trap less than chrysomyia megacephala (big head golden fly) kind come spawning.
Comparative example 2
Operation is grouped into by each group of following percetage by weight with embodiment 1, the except for the difference that described compost that traps: wheat bran
80% and imported fish meal 20%.Experiment finds using as above trapping the kind that the ovum that compost traps is not resistance to nicotine.
Embodiment 7
The chrysomyia megacephala (big head golden fly) kind that the embodiment of the present invention 1 to 3 is screened, embodiment 4 to 6 tolerate 3 ‰ through culture
Chrysomyia megacephala (big head golden fly) kind after the chrysomyia megacephala (big head golden fly) kind of nicotine formulation material, comparative example 1 and 2 are processed carries out resistance to nicotine aptitude tests, surveys
Examination process is:
The ovum 0.5g that chrysomyia megacephala (big head golden fly) adult after taking described embodiment culture to process respectively is produced, is respectively connected to 1kg weight
(wheat bran 80%, imported fish meal 20%, use mass percent concentration to the formulation material of the nicotine aqueous solution of percent concentration containing better quality
It is 60% that above-mentioned formulation material is adjusted to humidity by 3.0 ‰ nicotine aqueous solution) in, test result is shown in Table 1:
Table 1
| Process | Larvae development go through the phase (my god) | Larva bar number/g(bar) | Survival rate of larvae (%) |
| Embodiment 1 is initially planted | 7 | 26 | 63 |
| Embodiment 2 is initially planted | 7 | 24 | 66 |
| Embodiment 3 is initially planted | 7 | 25 | 64 |
| Comparative example 1 | —— | —— | —— |
| Comparative example 2 | 8 | 66 | 13 |
| Embodiment semi-finals kind | 5 | 17 | 95 |
| Embodiment 5 strengthening kind | 5 | 18 | 94 |
| Embodiment 6 strengthening kind | 5 | 19 | 93 |
Test result illustrates, comparative example 1,2 is not resistant to the nicotine aqueous solution of mass percent concentration 3.0 ‰.
1 to 3 chrysomyia megacephala (big head golden fly) kind through screening of embodiment is modulated in the nicotine aqueous solution of mass percent concentration 3.0 ‰
Formulation material in there occurs diapause phenomenon, larval weight is lighter, and has larger amount of death.The embodiment of the present invention 4 to 6 is through this
The resistance to nicotine ability of the chrysomyia megacephala (big head golden fly) kind after bright method hot housing process greatly improves, and can tolerate percent mass well
The formulation material of the nicotine aqueous solution modulation of specific concentration 3.0 ‰.
Claims (6)
1. a kind of intensifying method of the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind is it is characterised in that comprise the steps:
Starting stage: the ovum of the chrysomyia megacephala (big head golden fly) of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material is by following
The group of each weight fraction is grouped into: wheat bran 70～85%, imported fish meal 15～30%, with the nicotine of mass percent concentration 1 ‰
It is 60% that above-mentioned initial incubation material is adjusted to humidity by aqueous solution；In room conditions, by above-mentioned ovum cultivate to larva aging, change
After pupa, emergence, feed its adult；
1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced is entered using the 1st hot housing material
Row hot housing；Described 1st hot housing material is grouped into by each group of following weight fraction: wheat bran 70～85%, enters
Mouth fish flour 15～30%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned hot housing material being adjusted to humidity is
60%, s are selected from 0.1～0.5；In room conditions, by above-mentioned ovum cultivate to larva aging, pupate, sprout wings after, feed its one-tenth
Worm；
(n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained, and
Component contained by compost used by (n+1)th strain meets following ratio: the mass percent concentration of nicotine aqueous solution
=(mass percent concentration+s ‰ of nicotine in nicotine aqueous solution in n-th hot housing material), remaining constituent content is constant, n
It is the natural number more than or equal to 1, repeat to strengthen, until nicotine aqueous solution in compost described in last strain
Till mass percent concentration reaches 3 ‰；
The screening technique of the described chrysomyia megacephala (big head golden fly) of resistance to nicotine kind is: s1. configuration traps compost, will trap compost loading and open
In mouth container；The described compost that traps is grouped into by each group of following weight fraction: wheat bran 70～85%, imported fish meal 15
～30%, trap compost to be adjusted to humidity be 60% by above-mentioned with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰；
S2., when weather is fine, the container that will be equipped with trapping compost in the morning is put in house refuse heap, chicken house and pig farm to lure respectively
Collect wild fly blow, withdraw the container being put in everywhere in the afternoon；
S3. by reclaim each container be placed on indoor cultivation, wait the wild fly blow trapping cultivate to larva aging when,
Fly larvae is separated with trapping compost from food phase behavior using Chrysomya megacephala maggot；
S4. pass through the Morphological Identification of chrysomyia megacephala (big head golden fly) larva, choose Chrysomya megacephala maggot；
S5. 4～5 days chrysomyia megacephala (big head golden fly)s aging, that every fly larvae weighs 0.05～0.07g, figure is plump, color is golden yellow are filtered out
Maggot, at ambient temperature, allow Chrysomya megacephala maggot naturally to pupate, sprout wings after can obtain the chrysomyia megacephala (big head golden fly) kind of resistance to nicotine.
2. according to claim 1 intensifying method it is characterised in that described indoor conditionss be 25～30 DEG C of temperature, humidity
30～70%.
3. according to claim 1 intensifying method it is characterised in that the collocation method of described initial incubation material is will be initial
After each component of compost mixes, add the nicotine aqueous solution of certain concentration that the humidity of compost is adjusted to 60%.
4. according to claim 1 intensifying method it is characterised in that in every 1 kg initial incubation material access chrysomyia megacephala (big head golden fly)
Ovum 0.5～1.0g；Chrysomyia megacephala (big head golden fly) ovum 0.5～1.0 g is accessed in every 1 kg hot housing material.
5. according to claim 1 intensifying method it is characterised in that its adult of described nursing be all using whole milk powder,
Brown sugar and water are fed.
6. according to claim 5, intensifying method is it is characterised in that according to percent by weight, described whole milk powder, red
The usage ratio of sugar is whole milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with water using sponge, sucks for adult.
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