CN104171740A - Formula for trapping wild ptecticus aurifer for spawning and screening method of nicotine-resisting ptecticus aurifer variety - Google Patents
Formula for trapping wild ptecticus aurifer for spawning and screening method of nicotine-resisting ptecticus aurifer variety Download PDFInfo
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Abstract
The invention relates to the technical field of screening of animal varieties, and particularly relates to a formula for trapping wild ptecticus aurifer for spawning and a screening method of nicotine-resisting ptecticus aurifer variety. According to the formula, the product comprises the following components in percentage by weight: 70-90 percent of bran and 10-30 percent of imported fish powder, and the components are blended by using a nicotine water solution with a mass percent concentration of 0.1-0.2 percent until the humidity is 70 percent. According to the formula, the nicotine water solution with the proper concentration is directly adopted to be scientifically combined with the bran and the imported fish meal, the obtained formula successfully covers the smell and the bitter taste of the nicotine, and thus the wild ptecticus aurifer is effectively trapped for spawning; meanwhile, because of appropriateness and certainty of the nicotine content, the nicotine resistance capabilities of the wild ptecticus aurifer larva, obtained by different batches and same weights of the components are basically the same in the same trapping environment, and the stable screening effect is obtained.
Description
Technical field
The present invention relates to animal varieties triage techniques field, be specifically related to a kind of collection wild golden yellow phalangeal process stratiomyiid formulation material of laying eggs and screening technique of the golden yellow phalangeal process stratiomyiid of resistance to nicotine kind of luring.
Background technology
Inapplicable fresh tobacco leaf refers to not be suitable for the new fresh tobacco leaf (water content 80～90%) of cured tobacco production, different from the waste/hypo-tobacco leaf producing in the during production process of flue-cured tobacco of mentioning in a large number in document or discarded tobacco leaf (water content approximately 15%).Inapplicable tobacco leaf typically refers to cigarette strain and normally binds and remove after 2～3 milk pin leaves, cigarette industry is inapplicable, need remove in field bottom leaf and the upper leaf of processing, mainly comprise cigarette strain bottom because of illumination deficiency, depauperation, inclusion is not enough, estimate roasting after inferior quality, do not possess 2 bottom leaves that baking is worth, and hack at cigarette strain top, and bad, curtailment, structure are stiff, 2 tops of roasting rear inferior quality.
If these inapplicable tobacco leaves arbitrarily abandon field, very easily cause the superinfection of environmental pollution and tobacco virus, if adopt the concentrated destruction of prior art or the processing mode of landfill to need to spend a large amount of expenses, the disposal cost of industry inapplicable tobacco leaf per ton is on average up to 200 yuan at present, each tobacco leaf producing region often need to be under the organization leadership of government of local party committee, carry out policy propaganda, tabulation registration, build treatment facility, unifiedly remove, record file, publicity, cash the measures such as subsidy and supervise the processing of carrying out inapplicable tobacco leaf, the expense that wastes time and energy cost.
Adopting the insect of resistance to nicotine to carry out a biological disposal upon to inapplicable tobacco leaf is a kind of good alternate process method, environmental protection, and economic benefit is high.In order to utilize insect to realize the inapplicable fresh tobacco leaf of biological treatment, first need to filter out the insect species of resistance to nicotine.
The applicant, based on to the studying for a long period of time of housefly and inapplicable tobacco leaf treatment technology, sums up and utilizes inapplicable tobacco leaf housefly to be carried out to the method for screening, strengthening and the cultivation of resistance to nicotine strain.Described screening is specifically mixed with formulation material with 30～50% inapplicable tobacco leaf, 30～60% wheat bran, 0～10% imported fish meal (and/or dregs of beans of 0～10%), lure the wild housefly of collection to come to lay eggs, and by its larvae cultivation to aging state, according to the Growth and development index of larva, filter out the housefly kind of resistance to nicotine again.
Although this technical scheme can successfully filter out the housefly kind of resistance to nicotine, the resistance to nicotine ability of the mature larva that different batches screening obtains is distinguished to some extent, has the uncertainty of effect.Main cause is the application aspect of inapplicable tobacco leaf, although sum up through creationary research and great many of experiments the inapplicable tobacco leaf that adds zone of reasonableness in formulation material, can guarantee the control to common house-fly grub toxic action, but the factor that affects inapplicable nicotine content of tobacco leaves is very various, complicated mechanism, more difficult realization is accurately controlled.The factor that affects nicotine content in tobacco leaf mainly comprises: (1) different growing stages, in tobacco leaf, contained nicotine content is different: in tobacco seed, nicotine content is 0, in seedling raising process, rise and start to form at root, after developing along with root system, synthetic and the accumulation ability of nicotine strengthens gradually, so different growing stages, in tobacco leaf, contained nicotine content is different; (2) cigarette strain different parts, nicotine content is significantly different, and nicotine content is also subject to leaf position and the larger impact of the number of sheets; (3) the contained nicotine amount of different cultivars tobacco leaf is different; (4) weather conditions of soil dry-wet etc. all have the impacts of different savings on nicotine content; (5) difference of agronomic measures significantly affects nicotine content of tobacco leaves, comprise fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and root fertilizer, picking methods etc. the factor of cutting all significantly affects the nicotine content in tobacco leaf, take fertilization type as example, in the nitrogenous fertilizer of three kinds of forms, after urea is used, nicotine accumulation is the highest, and ammonium nitrate takes second place, the nicotine accumulation that potassium nitrate is processed is minimum, lower by 27% than using urea; Take the period of pinching as example, and Zhang Dan etc. study discovery, with postponing nicotine accumulation intensity peak the period of pinching, decline successively, and nicotine content also lowers successively, and every postponement is pinched for one day, and nicotine content just declines 0.0504%; Take again and cut root fertilizer as example, cut root 1/8 and compare with not cutting root, 1.9 percentage points of ranges of decrease 31.33% of upper leaf nicotine decline, middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
How avoiding the uncertainty that adopts different batches compost to screen the mature larva effect obtaining is this area technical barrier urgently to be resolved hurrily.And adopt, stablizing the resistance to nicotine insect strain that feasible technical scheme filters out more kinds, is that this area needs the important technological problems solving simultaneously.
Summary of the invention
The technical problem to be solved in the present invention is the defect that lacks the golden yellow phalangeal process stratiomyiid of resistance to nicotine kind in prior art in order to overcome, the screening technique of the golden yellow phalangeal process stratiomyiid of a kind of resistance to nicotine kind is provided, the inventive method is not affected by inapplicable tobacco leaf various factors, can guarantee to obtain stable screening effect.
Object of the present invention is achieved by the following technical programs:
Golden yellow phalangeal process stratiomyiid is the insect that is different from housefly, its life habit and different to the nutritional need of compost and housefly, a kind of important economic insects and scavenger insect, can utilize golden yellow phalangeal process stratiomyiid to process various corrupt organic wastes, as beer slag, bean dregs, chicken manure, pig manure and changing food waste etc.Existing golden yellow phalangeal process stratiomyiid kind all has good biological treatment ability to nontoxic organic waste, but not good to the treatment effect of inapplicable fresh tobacco leaf.Main cause is because contain the toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, the effect that golden yellow phalangeal process stratiomyiid larva (golden yellow phalangeal process stratiomyiid larva) is had certain murder by poisoning and suppresses to grow.In order to utilize the golden yellow phalangeal process stratiomyiid inapplicable fresh tobacco leaf of carrying out a biological disposal upon, first need from wild golden yellow phalangeal process stratiomyiid kind, filter out the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine, improve the tolerance of golden yellow phalangeal process stratiomyiid to fresh tobacco leaf.
The invention provides a kind of formulation material that lures the wild golden yellow phalangeal process stratiomyiid of collection to lay eggs, described formulation material is comprised of each component of following percetage by weight: wheat bran 70～90% and imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned mixed formulation material being adjusted to humidity is to contain water 70kg in the every 100kg compound of 70%().
Preferably, described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Described nicotine can adopt chemical pure nicotine reagent (purity >=99.5%).
The compound method of described formulation material is: after wheat bran and imported fish meal are mixed in proportion, adding the nicotine aqueous solution that above-mentioned formulation material is adjusted to humidity is 70%.
The present invention provides the screening technique of the golden yellow phalangeal process stratiomyiid of a kind of resistance to nicotine kind simultaneously, comprises the steps:
S1. the formulation material that preparation contains certain concentration nicotine, packs formulation material in open containers into;
The described formulation material that contains certain concentration nicotine is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
When S2. weather is fine, the container that formulation material is housed is put in respectively to house refuse heap, chicken house or pig farm and lures the wild golden yellow phalangeal process stratiomyiid ovum of collection; Preferably, at 9:00～16:00, can the container that formulation material is housed be put in respectively to house refuse heap, chicken house or pig farm in 8:00～9:00 in the morning, afternoon, 17:00～18:00 regained;
S3. each container of regaining is placed on to indoor cultivation, wait lure collection to wild golden yellow phalangeal process stratiomyiid ovum cultivate when aging to larva, utilize the larva is separated with formulation material from food phase behavior of golden yellow phalangeal process stratiomyiid larva;
S4. by the Morphological Identification of golden yellow phalangeal process stratiomyiid larva, choose golden yellow phalangeal process stratiomyiid larva;
S5. filter out 20～25 days aging, every larvas and weigh 0.10～0.16g, figure is plump, color is pitch-black golden yellow phalangeal process stratiomyiid larva, at ambient temperature, can obtain the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine after allowing golden yellow phalangeal process stratiomyiid larva naturally pupate, to sprout wings.
Preferably, the condition of culture of indoor cultivation is 25～30 ℃ of temperature described in S3, humidity 30～70%.
What utilize golden yellow phalangeal process stratiomyiid larva from food phase behavior by the larva concrete grammar separated with formulation material is: larva, cultivate after 20 days, Culture basin is placed in to a larger plastic tub that dry system chaff powder is housed, golden yellow phalangeal process stratiomyiid larva climbs voluntarily from formulation material, fall in the plastic tub that system chaff powder is housed, reach the larva object separated with formulation material.
Preferably, the container described in S2 is the square plastic feed basin that length is respectively 45cm, 30cm, 12cm, and the formulation material that 1～1.5 kg is modulated packs into and in this square plastic feed basin, is used for luring the wild golden yellow phalangeal process stratiomyiid ovum of collection.
The invention has the beneficial effects as follows:
The present invention directly adopts the nicotine aqueous solution and wheat bran and the imported fish meal science compatibility of suitable concentration, the formulation material obtaining is not only successfully covered the unpleasant and bitter taste of nicotine, effectively lure the wild golden yellow phalangeal process stratiomyiid of collection to come to lay eggs, and because appropriateness and the certainty of nicotine content, guarantee in same luring in collection environment, the golden yellow phalangeal process stratiomyiid larva of the formulation material that the same weight of different batches forms lures collection effect basic identical, obtains stable screening effect.
Formulation material nutritional labeling of the present invention is suitable, and golden yellow phalangeal process stratiomyiid larval growth is smooth, can will lure the golden yellow phalangeal process stratiomyiid larvae cultivation that arrives of collection to aging state, then according to the Growth and development index of larva, filter out the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine.
The inventive method is subject to tobacco planting season, tobacco planting area, collection and the transportation of inapplicable tobacco leaf, the impact of the factors such as place of banking up is less.
The specific embodiment
Below in conjunction with specific embodiment, further describe the present invention.Unless stated otherwise, reagent, the equipment that the present invention adopts is the conventional reagent of the art and equipment.Unless stated otherwise, wheat bran, imported fish meal and nicotine that the embodiment of the present invention adopts are conventional commercial raw material, and nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
embodiment 1
When S1. weather is fine, the formulation material (lure and gather materials) that preparation contains certain concentration nicotine, the described formulation material that contains certain concentration nicotine is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S2. by the above-mentioned formulation material modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm) into.By the morning on the same day, 8:00 was put in the plastic tub that formulation material is housed one life rubbish heap, afternoon, 17:00 regained plastic tub everywhere, took back indoor cultivation.
S3. (25 ℃ of temperature under indoor conditions, humidity 30%), larva, cultivate after 20 days, Culture basin is placed in to a larger plastic tub that dry system chaff powder is housed, golden yellow phalangeal process stratiomyiid larva climbs voluntarily from formulation material, fall in the plastic tub that system chaff powder is housed, reach the larva object separated with formulation material.
S4. under anatomical lens by the Morphological Identification of larva, with tweezers, choose separately golden yellow phalangeal process stratiomyiid larva, get rid of other fly kinds.
S5. filter out 20～25 days mature larvas, every larva weighs 0.10～0.16 g, figure is plump, color is pitch-black golden yellow phalangeal process stratiomyiid larva, and counting.At ambient temperature, can obtain the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine after allowing these golden yellow phalangeal process stratiomyiid larvas naturally pupate, to sprout wings.
embodiment 2
Other operations are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Finishing screen is selected 20～25 days aging, every larvas and is weighed 0.10～0.16 g, figure is plump, color is pitch-black golden yellow phalangeal process stratiomyiid larva, at ambient temperature, can obtain the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine after allowing these golden yellow phalangeal process stratiomyiid larvas naturally pupate, to sprout wings.
embodiment 3
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 2.0 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 4
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 5
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 6
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 7
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 8
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
embodiment 9
Other are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
comparative example 1
Other operations are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 80%.
The not beneficial consequence that this comparative example formula produces is: owing to luring, the initial humidity that gathers materials is higher, material fermenting speed is slower, can not produce in time the smell of enough rotten organic matters, can not lure and lure the same day of gathering materials collection to lay eggs to wild golden yellow phalangeal process stratiomyiid is sudden in placement.
comparative example 2
Other operations are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 55%.
The not beneficial consequence that this comparative example formula produces is: owing to luring, the initial humidity that gathers materials is lower, material fermenting speed is slow, and material surface is easily dry, can not produce in time the smell of enough rotten organic matters, cause luring and luring the same day of gathering materials collection to come to lay eggs less than wild golden yellow phalangeal process stratiomyiid in placement.
comparative example 3
Other operations are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 0.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
The not beneficial consequence that this comparative example formula produces is: with this formula, likely lure collection also to come to lay eggs to the wild golden yellow phalangeal process stratiomyiid of resistance to nicotine not, affect the wild golden yellow phalangeal process stratiomyiid kind of resistance to nicotine lured to collection effect.
comparative example 4
Other operations are with embodiment 1, and different is described formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 2.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
The not beneficial consequence that this comparative example formula produces is: owing to luring, the initial nicotinic density in gathering materials is too high, and toxicity is excessively strong, causes the wild golden yellow phalangeal process stratiomyiid larva mortality that lures collection to arrive, and impact lures collection effect to the golden yellow phalangeal process stratiomyiid of resistance to nicotine kind.
comparative example 5
Described formulation material is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with clear water, above-mentioned formulation material being adjusted to humidity is 70%.
Utilize the method for golden yellow phalangeal process stratiomyiid kind that formulation material as mentioned above screens resistance to nicotine with embodiment 1.
The not beneficial consequence that this comparative example formula produces is:
Although this formula can lure collection to come to lay eggs to wild golden yellow phalangeal process stratiomyiid adult, but in the formulation material of the golden yellow phalangeal process stratiomyiid ovum access that its offspring is produced with the nicotine aqueous solution modulation of mass percent concentration 2.0 ‰, due to toxic action of nicotine etc., can not complete normal growth and development process.
embodiment 10
The resistance to nicotine aptitude tests experiment of the golden yellow phalangeal process stratiomyiid kind that embodiment 1～9 and comparative example 1～5 screen:
Resistance to nicotine aptitude tests process is: the golden yellow phalangeal process stratiomyiid ovum that the golden yellow phalangeal process stratiomyiid kind second generation adult that embodiment 1～9 and comparative example 1～5 are screened produces accesses respectively the formulation material that nicotinic density is higher (wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%), every 1.5 kg pack in a square plastic feed basin, and it is 1.5g that every basin connects ovum amount.(30 ℃ of temperature under indoor conditions, humidity 60%), larva, cultivate after 20 days, Culture basin is placed in to a larger plastic tub that dry system chaff powder is housed, golden yellow phalangeal process stratiomyiid larva climbs voluntarily from formulation material, fall in the plastic tub that system chaff powder is housed, reach the larva object separated with formulation material.
Test result is: it is 20～25 days that the golden yellow phalangeal process stratiomyiid kind larvae development that embodiment 1～9 filters out is gone through the phase, approximately 0.10～0.16 gram of every mature larva body weight, figure is plump, color is pitch-black, at ambient temperature, can naturally pupate and sprout wings, the growing state of different embodiment is basic identical, the formulation material that embodiment 1～9 preparation is described can well lure the golden yellow phalangeal process stratiomyiid kind that collects and screen resistance to nicotine, and can guarantee appropriateness and the certainty of nicotine content, same luring in collection environment, the golden yellow phalangeal process stratiomyiid larval growth situation that the homogenous quantities formulation material of different batches obtains is basic identical, obtain stable screening effect.The formulation material of comparative example 1～5 preparation can not lure collection or screen the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine.Be shown in Table 1.
Table 1
Claims (7)
1. one kind lures the wild golden yellow phalangeal process stratiomyiid of the collection formulation material of laying eggs, it is characterized in that, the described formulation material that contains certain concentration nicotine is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
2. the formulation material that lures according to claim 1 the wild golden yellow phalangeal process stratiomyiid of collection to lay eggs, it is characterized in that, each component that the described formulation material that contains certain concentration nicotine contains following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
3. the formulation material that lures according to claim 2 the wild golden yellow phalangeal process stratiomyiid of collection to lay eggs, it is characterized in that, each component that the described formulation material that contains certain concentration nicotine contains following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
4. a screening technique for the golden yellow phalangeal process stratiomyiid of resistance to nicotine kind, is characterized in that, comprises the steps:
S1. the formulation material that preparation contains certain concentration nicotine, packs formulation material in open containers into;
Each component that the described formulation material that contains certain concentration nicotine contains following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
When S2. weather is fine, 8:00～9:00 was put in respectively house refuse heap, chicken house or pig farm by the container that formulation material is housed and lured the wild golden yellow phalangeal process stratiomyiid ovum of collection the morning, and 17:00～18:00 regained and was put in container everywhere afternoon;
S3. each container reclaiming is placed on to indoor cultivation, wait lure collection to wild golden yellow phalangeal process stratiomyiid ovum cultivate when aging to larva, utilize the larva is separated with formulation material from food phase behavior of golden yellow phalangeal process stratiomyiid larva;
S4. by the Morphological Identification of golden yellow phalangeal process stratiomyiid larva, choose golden yellow phalangeal process stratiomyiid larva;
S5. filter out 20～25 days aging, every larvas and weigh 0.10～0.16g, figure is plump, color is pitch-black golden yellow phalangeal process stratiomyiid larva, at ambient temperature, can obtain the golden yellow phalangeal process stratiomyiid kind of resistance to nicotine after allowing golden yellow phalangeal process stratiomyiid larva naturally pupate, to sprout wings.
5. screening technique according to claim 4, is characterized in that, the condition of culture of indoor cultivation is 25～30 ℃ of temperature described in S3, humidity 30～70%.
6. screening technique according to claim 4, it is characterized in that, what described in S3, utilize golden yellow phalangeal process stratiomyiid larva from food phase behavior by the larva concrete grammar separated with formulation material is: larva, cultivate after 20 days, Culture basin is placed in to a larger plastic tub that dry system chaff powder is housed, golden yellow phalangeal process stratiomyiid larva climbs voluntarily from formulation material, falls in the plastic tub that system chaff powder is housed.
7. screening technique according to claim 4, it is characterized in that, the compound method of the described formulation material that contains certain concentration nicotine is: after wheat bran and imported fish meal are mixed in proportion, adding the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰ that above-mentioned formulation material is adjusted to humidity is 70%.
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|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN103563858A (en) * | 2013-11-07 | 2014-02-12 | 南雄市中大赛尔生物技术有限公司 | Reinforcing method for nicotine-resistant housefly species |
| CN103975902A (en) * | 2014-05-30 | 2014-08-13 | 中山大学 | Method for attracting wild hermetia illucens |
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Application publication date: 20141203 |