CN104280328B - Flow cytometry analysis device and method - Google Patents
Flow cytometry analysis device and method Download PDFInfo
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- CN104280328B CN104280328B CN201410280220.4A CN201410280220A CN104280328B CN 104280328 B CN104280328 B CN 104280328B CN 201410280220 A CN201410280220 A CN 201410280220A CN 104280328 B CN104280328 B CN 104280328B
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Abstract
The invention discloses a flow cytometry analysis device and method. The analysis device comprises a loading detection device, a sheath fluid device and a pressure supply device, wherein the loading detection device comprises a sample container, a loading pipeline, a flow detector and a flow chamber; the sample container is used for containing a sample solution and is sealed by virtue of a sealing piece; one end of the loading pipeline is connected with the sample container, the flow detector is arranged on the loading pipeline, and the flow chamber is connected with the other end of the loading pipeline; the sheath fluid device comprises a sheath fluid container, a sheath fluid pipeline and a first valve device; one end of the sheath fluid pipeline is connected with the sheath fluid container; the first valve device is arranged on the sheath fluid pipeline; the pressure supply device comprises an air source, a first air pressure pipeline and a second air pressure pipeline; the first air pressure pipeline is connected between the air source and the sample container; and the second air pressure pipeline is connected between the air source and the sheath fluid container. According to the flow cytometry analysis device provided by the invention, the problem of low analysis accuracy is solved on the basis of low cost and convenience in use.
Description
Technical field
The present invention relates to cell analysis technical field, more particularly, to a kind of flow cytometry device and analysis method.
Background technology
Generally use streaming in blood cell/field of cell analysis and focus on art, unicellular stream will be constrained to by sample cell,
Through sensor, irradiated by laser and detect or electric field between electrodes impedance variation, produce the pulse of different amplitudes and quantity, thus right
Cell carries out counting the analysis with information such as sizes.
The central principle that streaming focuses on art is pipeline lamina flow principle, and fluid is by two parts: sample flow and sheath stream form, in pipe
In road, sheath stream wraps up sample flow through sensor, clear-cut wherein between sheath stream and sample flow, and meets the speed of flow measurement
Gradient distribution.
Flow cytometry device of the prior art generally adopts syringe pump or peristaltic pump to drive sample flow and sheath stream.So
And, easily cause the fluctuation of sheath hydraulic coupling using syringe pump or peristaltic pump, lead to the fluctuation of sheath flow quantity, and then affect sample flow
Stability.
In addition, flow cytometry device of the prior art, normalized particle measurement method is usually used, adds in the sample
Enter the particle of concentration known, mix with the sample of known volume by the standard particle of concentration known, the standard grain in mixed liquor
Sub- concentration can calculate.Machine on mixed liquor is measured, draws standard particle number, you can inverse goes out tested volume, reaches
Purpose to blood cell absolute counting.However, this method by concentration known normalized particle inverse sample volume needs
User accurately allots the concentration of particle, user is required higher, in-convenience in use, and increased the use cost of user.
Therefore, how solve precision of analysis that Almost Sure Sample Stability difference leads to relatively low and in order to realize blood simultaneously
The purpose of cell absolute counting, and lead to cost to raise and awkward problem, becoming those skilled in the art will solve
Technical problem.
Content of the invention
The invention provides a kind of flow cytometry device, it can ensure cost basis relatively low and easy to use
On, solve the problems, such as that precision of analysis is relatively low.Present invention also offers a kind of flow cytometric analysis.
A kind of flow cytometry device that the present invention provides, comprising:
Sample detection device, described sample detection device includes being sealed for housing sample liquid and by sealing member
Loading pipeline that sample container, one end are connected with described sample container, the flow detection meter being arranged on described loading pipeline
And the flow chamber being connected with the other end of described loading pipeline, sample liquid is in the indoor formation sample flow of described flowing, and institute
State flow chamber and be provided with sample flow analytical equipment；
Sheath liquid device, the sheath liquid pipeline that described sheath liquid device includes sheath liquid container, one end is connected with described sheath liquid container
And it is arranged at the first valving on described sheath liquid pipeline, the other end of described sheath liquid pipeline is connected with described flow chamber
Connect, so that sheath fluid and sample liquid are in the indoor described sample flow of formation of described flowing；
Pressure supply unit, described pressure supply unit include source of the gas, be connected to described source of the gas and described sample container it
Between the first pneumatic circuit and the second pneumatic circuit being connected between described source of the gas and described sheath liquid container, described first air pressure
Pipeline is provided with loading pressure-regulating device and loading pressure transducer, and described second pneumatic circuit is provided with sheath hydraulic coupling and adjusts
Device and sheath fluid pressure transducer.
Preferably, described sealing member is cleaning swab, and described loading pipeline passes through described cleaning swab by loading pin, and
Described cleaning swab is coordinated for slipper seal with described loading pin；Described flow cytometry device also includes cleaning device, institute
State the detergent line that cleaning device includes being connected between described cleaning swab and described sheath liquid container, described detergent line sets
There is the second valving.
Preferably, also waste liquid recovery apparatus are included, described waste liquid recovery apparatus include waste liquid barrel, are connected to described cleaning and wipe
The first waste-solution line between sub and described waste liquid barrel, the 3rd valving being arranged at described first waste-solution line and waste liquid
Pump.
Preferably, described waste liquid recovery apparatus also include waste liquid pool, are connected between described flow chamber and described waste liquid pool
The second waste-solution line and be arranged at the 4th valving of described second waste-solution line, described second waste-solution line and described the
One waste-solution line is connected, and junction point is located between described 3rd valving and described waste drains pump.
Present invention also offers a kind of flow cytometric analysis, including step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure to be more than described sheath hydraulic coupling, so that sample
Liquor stream enters in sheath fluid, forms sample flow；
Adjust the initial pressure of loading pressure, so that loading pressure is maintained in set point, realize the coarse adjustment of loading pressure；
Sample flow is flow to the real-time traffic of flow chamber and target setting value is compared, if described real-time traffic is more than institute
State target setting value, then control described loading pressure drop, so that described real-time traffic levels off to described target setting value；If institute
State real-time traffic and be less than described target setting value, then control described loading pressure to rise, so that described real-time traffic levels off to institute
State target setting value；
Different time in each step above-mentioned obtains sample liquor stream to the real-time traffic of flow chamber；
Calculate sample liquor stream with the computational methods of integration to the flow summation of flow chamber, to calculate cell concentration.
A kind of flow cytometry device that the present invention provides, comprising: sample detection device, described sample detection device bag
Include the loading for housing sample liquid and the sample container that sealed by sealing member, one end are connected with described sample container
The flowing that pipeline, the flow detection meter being arranged on described loading pipeline and the other end with described loading pipeline are connected
Room, sample liquid is in the indoor formation sample flow of described flowing, and described flow chamber is provided with sample flow analytical equipment；Sheath liquid device, institute
State the sheath liquid pipeline that sheath liquid device includes sheath liquid container, one end is connected with described sheath liquid container and be arranged at described sheath liquid pipe
The first valving on road, the other end of described sheath liquid pipeline is connected with described flow chamber, so that sheath fluid and sample liquid exist
The described flowing interior described sample flow of formation；Pressure supply unit, described pressure supply unit includes source of the gas, is connected to described gas
The first pneumatic circuit between source and described sample container and the second gas being connected between described source of the gas and described sheath liquid container
Pressure pipe road, described first pneumatic circuit is provided with loading pressure-regulating device and loading pressure transducer, described second pneumatic tube
Road is provided with sheath fluid pressure-regulating device and loading pressure transducer.
It is arranged such, the flow cytometry device that the present invention provides, sample liquid energy is enough to be delivered to stream according to target flow
Dynamic room, is included by sheath fluid, is formed and stablizes sample flow to be checked, described in detail below.Source of the gas passes through the first pneumatic circuit to be held to sample
Device provides loading pressure, provides sheath hydraulic coupling by the second pneumatic circuit to sheath liquid container, so that sample liquid and sheath fluid are in pressure
Effect dirty enter flowing indoor, form sample flow, loading pressure-regulating device and sheath fluid pressure-regulating device can be to loading pressures
Power and sheath hydraulic coupling are adjusted respectively, during loading, by loading pressure-regulating device and the control of sheath fluid pressure-regulating device
Sample pressure is more than sheath hydraulic coupling, so that sample liquor stream enters in sheath fluid, forms sample flow；Due to starting the loading stage, sample liquid
Loading pressure is larger, and it is more than set target pressure, forms the effect quickly pushing away sample.Then, in the detection of loading pressure transducer
Sample pressure, and compared with target flow value by the actual flow value comparing the detection of the sample flow detection meter on loading pipeline
Relatively, if actual flow value is more than target flow value, control loading pressure drop；If actual flow value is less than target flow value,
Loading pressure is then controlled to rise；The final loading pressure that adjusts makes actual flow value level off to target flow value.Loading pressure sensing
The loading pressure of device real-time detection practical, analysis personnel can make loading pressure be maintained at normal model with real-time monitoring loading pressure
In enclosing.So ensure that the stability of loading flow, in addition, during sample liquor stream to flow chamber, flow detection meter can
Obtain real-time traffic with periodic, the flow value that sample flow analytical equipment repeatedly obtains according to flow detection meter, according to integration
Computational methods, can calculate the total flow in special time, and then sample flow analytical equipment can be according to total stream of sample liquid
Amount is precisely calculated cell concentration.It is arranged such, sample flow quantity is to obtain in the case of stable, the cell analyzing
Concentration has higher accuracy.And, the cell analysis apparatus that the present invention provides, using source of the gas as loading pressure source and sheath
Hydraulic coupling source, it is to avoid using high accuracy pump of the prior art with the part that eliminates the pulse that pump produces, therefore, the present invention carries
For flow cytometry device, its can ensure cost relatively low and easy to use on the basis of, solution analysis precision relatively low
Problem.
Present invention also offers a kind of flow cytometric analysis, including step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure to be more than described sheath hydraulic coupling, so that sample
Liquor stream enters in sheath fluid, forms sample flow；
Adjust the initial pressure of loading pressure, so that loading pressure is maintained in set point, realize the coarse adjustment of loading pressure；
Sample flow is flow to the real-time traffic of flow chamber and target setting value is compared, if described real-time traffic is more than institute
State target setting value, then control described loading pressure drop, so that described real-time traffic levels off to described target setting value；If institute
State real-time traffic and be less than described target setting value, then control described loading pressure to rise, so that described real-time traffic levels off to institute
State target setting value.
Different time in each step above-mentioned obtains sample liquor stream to the real-time traffic of flow chamber；
Calculate sample liquor stream with the computational methods of integration to the flow summation of flow chamber, to calculate cell concentration.
So, the cell analysis method providing according to the present invention, ensure that the stability of the flow of sample liquid, and adopts
The flow summation calculated with the method for integration is more accurate, and then ensure that the degree of accuracy of cell concentration.
Brief description
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Have technology description in required use accompanying drawing be briefly described it should be apparent that, drawings in the following description be only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, acceptable
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the flow cytometry schematic device that the specific embodiment of the invention provides；
In figure:
Sample container 11, loading pipeline 12, flow detection meter 13, flow chamber 14, sheath liquid container 15, sheath fluid
Pipeline 16, the first valving 17, source of the gas 18, the first pneumatic circuit 19, the second pneumatic circuit 20, loading pressure
Adjusting means 21, loading pressure transducer 22, sheath fluid pressure-regulating device 23, sheath fluid pressure transducer 24, cleaning
Swab 25, detergent line 26, the second valving 27, waste liquid barrel 28, the first waste-solution line 29, the 3rd valve dress
Put 30, waste drains pump 31, waste liquid pool 32, the second waste-solution line 33, the 4th valving 34.
Specific embodiment
This specific embodiment provides a kind of flow cytometry device, and it can ensure that cost is relatively low and user
Just, on the basis of, solve the problems, such as that precision of analysis is relatively low.Present invention also offers a kind of flow cytometric analysis.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation description is it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of not making creative work
Embodiment, broadly falls into the scope of protection of the invention.
Refer to Fig. 1, the flow cytometry device that this specific embodiment provides, comprising:
Sample detection device, sample detection device is included for housing sample liquid and the sample being sealed by sealing member
Loading pipeline 12 that container 11, one end are connected with sample container 11, the flow detection meter 13 being arranged on loading pipeline 12 with
And the flow chamber 14 being connected with the other end of loading pipeline 12, sample liquid forms sample flow in flow chamber 14, and flow chamber
14 are provided with sample flow analytical equipment；
Sheath liquid device, the sheath liquid pipeline 16 that sheath liquid device includes sheath liquid container 15, one end is connected with sheath liquid container 15 with
And it is arranged at the first valving 17 on sheath liquid pipeline 16, the other end of sheath liquid pipeline 16 is connected with flow chamber 14, so that
Sheath fluid and sample liquid form sample flow in flow chamber 14；
Pressure supply unit, pressure supply unit include source of the gas 18, be connected between source of the gas 18 and sample container 11
One pneumatic circuit 19 and the second pneumatic circuit 20 being connected between source of the gas 18 and sheath liquid container 15, the first pneumatic circuit 19 sets
There are loading pressure-regulating device 21 and loading pressure transducer 22, described second pneumatic circuit 20 is provided with sheath hydraulic coupling and adjusts dress
Put 23 and sheath fluid pressure transducer 24.
It should be noted that above-mentioned loading pressure-regulating device 21 and sheath fluid pressure-regulating device 23 can be air pressure adjustment
Valve.
It is arranged such, the flow cytometry device that this specific embodiment provides, sample liquid energy is enough according to target flow
It is delivered to flow chamber 14, is included by sheath fluid, formed and stablize sample flow to be checked.Source of the gas 18 passes through the first pneumatic circuit 19 to be held to sample
Device 11 provides loading pressure, provides sheath hydraulic coupling by the second pneumatic circuit 20 to sheath liquid container 15, so that sample liquid and sheath fluid
Flow under pressure in flow chamber 14, form sample flow, loading pressure-regulating device 21 and sheath fluid pressure-regulating device 23
Loading pressure and sheath hydraulic coupling can be adjusted respectively, during loading, by loading pressure-regulating device 21 and sheath hydraulic coupling
Adjusting means 23 controls loading pressure to be more than sheath hydraulic coupling, so that sample liquor stream enters in sheath fluid, forms sample flow.
Due to starting the loading stage, the loading pressure of sample liquid is larger, and it is more than set target pressure, is formed and quickly pushes away sample
Effect.Then, loading pressure transducer detection loading pressure, and by comparing the inspection of the sample flow detection meter on loading pipeline
The actual flow value surveyed is compared with target flow value, if actual flow value is more than target flow value, controls loading pressure
Decline；If actual flow value is less than target flow value, loading pressure is controlled to rise；The final loading pressure that adjusts makes actual flow
Value levels off to target flow value.The loading pressure of loading pressure transducer real-time detection practical, analysis personnel can be with real-time monitoring
Loading pressure, makes loading pressure be maintained in normal range.So ensure that the stability of loading flow, in addition, in sample liquid
During flowing to flow chamber 14, flow detection meter 13 can periodically obtain real-time traffic, sample flow analytical equipment according to
The flow value of more than 13 acquisition of flow detection meter, according to integral, can calculate the total flow in special time, enter
And sample flow analytical equipment can be precisely calculated cell concentration according to the total flow of sample liquid, specifically can be as described below:
In this specific embodiment flow detection meter 13 can detect sample flow quantity scope from several microlitres per minute to several
Hectolambda is per minute, and therefore, when sample liquor stream is through flow detection meter 13, flow detection meter 13 can obtain current sample liquid
Flow；When starting measurement, trigger recording sample flow value q, and with the periodicity time, such as, when 0.1s time interval is
Between piece t, sample actual flow q when recording each timeslice, when system record completes, integration count each timeslice flow,
Final calculating cumulative volume vs, algorithm is as follows: vs=v1+v2+ ...+vt=(q1+q2+ ...+qt) t=0.1 × (q1+q2+ ...+
Qt), unit be microlitre；If when time record cell (particle) sum is a (individual), absolute counting calculates concentration results n and is: n=
a/(vs×10-6), unit is/liter；If sample has been diluted b times before measuring, concentration n '=bn, unit is/liter.
It is arranged such, sample flow quantity is to obtain in the case of stable, and the cell concentration analyzing has higher
Accuracy.And, the cell analysis apparatus that this specific embodiment provides, using source of the gas 18 as loading pressure source and sheath fluid
Pressure source, it is to avoid using high accuracy pump of the prior art with the part that eliminates the pulse that pump produces, therefore, the present invention provides
Flow cytometry device, its can ensure cost relatively low and easy to use on the basis of, solution analysis precision relatively low
Problem.
In addition, in the preferred version of this specific embodiment offer, sample container 11 is provided with the cleaning sealing engagement therewith
Swab 25, loading pipeline 12 passes through loading pin and passes through cleaning swab 25, and cleaning swab 25 is coordinated for slipper seal with loading pin；
Flow cytometry device also includes cleaning device, and cleaning device includes being connected between cleaning swab 25 and sheath liquid container 15
Detergent line 26, detergent line 26 is provided with the second valving 27.
It is arranged such, cleaning swab 25 and loading pin are slipper seal cooperation, it is to avoid the problem of sample container 11 pressure release,
Cleaning swab 25 can slide along loading pin, and loading pin can be carried out.During cleaning, open the second valving 27 and sheath
Hydraulic pressure apparatus for adjusting force 23, sheath fluid flow to cleaning swab 25 by detergent line 26, and cleaning swab 25 slides along loading pin, with right
The outer wall of loading pin is carried out.
In addition, the flow cytometry device that this specific embodiment provides, waste liquid recovery apparatus can also be included, described
Waste liquid recovery apparatus include waste liquid barrel 28, the first waste-solution line 29 being connected between cleaning swab 25 and waste liquid barrel 28, setting
The 3rd valving 30 and waste drains pump 31 in the first waste-solution line 29.
It is arranged such, when cleaning the outer wall of loading pin, the 3rd valving 30 and waste drains pump 31, waste liquid can be opened simultaneously
Pump 31 provides negative-pressure gas, the waste liquid after cleaning loading pin can be evacuated among waste liquid barrel 28, the waste liquid after cleaning is entered
Row reclaims.
Further, waste liquid recovery apparatus can also include waste liquid pool 32, be connected between flow chamber 14 and waste liquid pool 32
The second waste-solution line 33 and the 4th valving 34 being arranged at the second waste-solution line 33, the second waste-solution line 33 gives up with first
Liquid pipeline 29 is connected, and junction point is located between the 3rd valving 30 and waste drains pump 31.
It is arranged such, after measurement terminates, end the first pneumatic circuit 19 and sheath liquid pipeline 16 simultaneously, and open the 3rd valve
Door gear 30, now, pressure release in sample container 11；It is then turned on setting sheath fluid pressure-regulating device 23 to cleaning pressure,
Open waste drains pump 31 and the second valving 27, and now cleaning swab 25 moves downward, the negative pressure due to waste drains pump 31 is made
With forming the gas-liquid mixture of cleaning loading pin in cleaning swab 25, descending process is fully carried out to loading pin outer wall；Clearly
When washing the most advanced and sophisticated position that swab 25 reaches loading pin, close the second valving 27, and open the first valving 17, now,
The inwall of flow chamber 14 and loading pin, flow detection meter 13 is carried out, cleaning waste liquid is by waste drains pump 31 through the 3rd valve
Device 30 pump drainage is to waste liquid barrel 28.This kind of cleaning structure and method can with relatively little of consumption liquid measure and time-consuming complete efficient to pin
Cleaning.
This specific embodiment additionally provides a kind of flow cytometric analysis, including step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure to be more than described sheath hydraulic coupling, so that sample
Liquor stream enters in sheath fluid, forms sample flow；
Adjust the initial pressure of loading pressure, so that loading pressure is maintained in set point, realize the coarse adjustment of loading pressure；
Sample flow is flow to the real-time traffic of flow chamber and target setting value is compared, if described real-time traffic is more than institute
State target setting value, then control described loading pressure drop, so that described real-time traffic levels off to described target setting value；If institute
State real-time traffic and be less than described target setting value, then control described loading pressure to rise, so that described real-time traffic levels off to institute
State target setting value；
Different time in each step above-mentioned obtains sample liquor stream to the real-time traffic of flow chamber；
Calculate sample liquor stream with the computational methods of integration to the flow summation of flow chamber, to calculate cell concentration.
So, the cell analysis method providing according to this specific embodiment, ensure that the stablizing of flow of sample liquid
Property, and the flow summation calculated using the method for integration is more accurate, and then ensure that the degree of accuracy of cell concentration.
Described above to the disclosed embodiments, makes professional and technical personnel in the field be capable of or uses the present invention.
Multiple modifications to these embodiments will be apparent from for those skilled in the art, as defined herein
General Principle can be realized without departing from the spirit or scope of the present invention in other embodiments.Therefore, the present invention
It is not intended to be limited to the embodiments shown herein, and be to fit to and principles disclosed herein and features of novelty phase one
The scope the widest causing.
Claims (5)
1. a kind of flow cytometry device, comprising:
Sample detection device, described sample detection device is included for housing sample liquid and the sample being sealed by sealing member
Loading pipeline that container, one end are connected with described sample container, the flow detection meter being arranged on described loading pipeline and
The flow chamber being connected with the other end of described loading pipeline, sample liquid is in the indoor formation sample flow of described flowing, and described stream
Dynamic room is provided with sample flow analytical equipment；
Sheath liquid device, the sheath liquid pipeline that described sheath liquid device includes sheath liquid container, one end is connected with described sheath liquid container and
It is arranged at the first valving on described sheath liquid pipeline, the other end of described sheath liquid pipeline is connected with described flow chamber, with
Make sheath fluid and sample liquid in the indoor described sample flow of formation of described flowing；
It is characterized in that, also include:
Pressure supply unit, described pressure supply unit includes source of the gas, is connected between described source of the gas and described sample container
First pneumatic circuit and the second pneumatic circuit being connected between described source of the gas and described sheath liquid container, described first pneumatic circuit
It is provided with loading pressure-regulating device and loading pressure transducer, described second pneumatic circuit is provided with sheath fluid pressure-regulating device
With sheath fluid pressure transducer.
2. flow cytometry device as claimed in claim 1 is it is characterised in that described sealing member is cleaning swab, described
Loading pipeline passes through described cleaning swab by loading pin, and described cleaning swab is coordinated for slipper seal with described loading pin；
Described flow cytometry device also includes cleaning device, and described cleaning device includes being connected to described cleaning swab and described sheath
Detergent line between liquid container, described detergent line is provided with the second valving.
3. flow cytometry device as claimed in claim 2 is it is characterised in that also including waste liquid recovery apparatus, described useless
Liquid retracting device includes waste liquid barrel, the first waste-solution line being connected between described cleaning swab and described waste liquid barrel, is arranged at
3rd valving of described first waste-solution line and waste drains pump.
4. flow cytometry device as claimed in claim 3 is it is characterised in that described waste liquid recovery apparatus also include waste liquid
Pond, the second waste-solution line being connected between described flow chamber and described waste liquid pool and be arranged at described second waste-solution line
Four valvings, described second waste-solution line is connected with described first waste-solution line, and junction point is located at described 3rd valve
Between device and described waste drains pump.
5. a kind of flow cytometric analysis are it is characterised in that include step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure to be more than described sheath hydraulic coupling, so that sample liquor stream
Enter in sheath fluid, form sample flow；
Adjust the initial pressure of loading pressure, so that loading pressure is maintained in set point, realize the coarse adjustment of loading pressure；
Sample flow is flow to the real-time traffic of flow chamber and target setting value is compared, if described real-time traffic is more than described setting
Target value, then control described loading pressure drop, so that described real-time traffic levels off to described target setting value；If described reality
Shi Liuliang is less than described target setting value, then control described loading pressure to rise so that described real-time traffic level off to described in set
Target value；
Different time in each step above-mentioned obtains sample liquor stream to the real-time traffic of flow chamber；
Calculate sample liquor stream with the computational methods of integration to the flow summation of flow chamber, to calculate cell concentration.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59228147A (en) * | 1983-06-10 | 1984-12-21 | Hitachi Ltd | Particle measuring apparatus |
| EP0602416A1 (en) * | 1992-12-14 | 1994-06-22 | Becton, Dickinson and Company | Method for control of flow cytometer having vacuum driven flow |
| CN102854095A (en) * | 2011-06-27 | 2013-01-02 | 深圳理邦实验生物电子有限公司 | Detection device and method for stream mode particle |
| CN103575633A (en) * | 2012-08-10 | 2014-02-12 | 深圳迈瑞生物医疗电子股份有限公司 | Streaming type instrument and liquid path system thereof |
| CN103837462A (en) * | 2014-03-03 | 2014-06-04 | 中国科学院苏州生物医学工程技术研究所 | Miniature flow cytometer fluid path system |
-
2014
- 2014-06-20 CN CN201410280220.4A patent/CN104280328B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59228147A (en) * | 1983-06-10 | 1984-12-21 | Hitachi Ltd | Particle measuring apparatus |
| EP0602416A1 (en) * | 1992-12-14 | 1994-06-22 | Becton, Dickinson and Company | Method for control of flow cytometer having vacuum driven flow |
| CN102854095A (en) * | 2011-06-27 | 2013-01-02 | 深圳理邦实验生物电子有限公司 | Detection device and method for stream mode particle |
| CN103575633A (en) * | 2012-08-10 | 2014-02-12 | 深圳迈瑞生物医疗电子股份有限公司 | Streaming type instrument and liquid path system thereof |
| CN103837462A (en) * | 2014-03-03 | 2014-06-04 | 中国科学院苏州生物医学工程技术研究所 | Miniature flow cytometer fluid path system |
Non-Patent Citations (1)
| Title |
|---|
| 《流式细胞仪及其临床应用》;刘波;《中国医疗设备》;20131231;第28卷(第07期);第66-69页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110118716A (en) * | 2019-05-28 | 2019-08-13 | 北京唯公医疗技术有限公司 | Liquid fluid system, air-tightness detection method and the flow cytometer of flow cytometer |
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| CN104280328A (en) | 2015-01-14 |
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