CN104277970B - The apparatus and method of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication - Google Patents
The apparatus and method of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication Download PDFInfo
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- CN104277970B CN104277970B CN201310270940.8A CN201310270940A CN104277970B CN 104277970 B CN104277970 B CN 104277970B CN 201310270940 A CN201310270940 A CN 201310270940A CN 104277970 B CN104277970 B CN 104277970B
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Abstract
The invention discloses the apparatus and method of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication, described device comprises the first conductive carbon fibre pole plate and the second conductive carbon fibre pole plate that are oppositely arranged, first conductive carbon fibre pole plate is connected with stepper-motor, second conductive carbon fibre pole plate is connected with stadimeter, and the first conductive carbon fibre pole plate and the second conductive carbon fibre pole plate and direct supply and switch form an electric field loop, a described electric field loop electric field monitoring device in parallel; Described first conductive carbon fibre pole plate and the second conductive carbon fibre pole plate are arranged in a magneticfield coil, described magneticfield coil is placed in an airtight transparent vessel, the two ends of described magneticfield coil are connected with magnetic field power supply respectively, and described method is the method being separated based on said apparatus electrogenesis bacterium, growing and taming.The present invention can improve the efficiency of anode of microbial fuel cell electrogenesis bacteria distribution, growth and domestication.
Description
Technical field
The present invention relates to energy environment technical field, particularly, the present invention relates to the apparatus and method of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication.
Background technology
Along with the high speed development of human society, the conventional fossil fuel such as coal, oil is on the verge of exhaustion, is difficult to the survival and development of maintaining the mankind for a long time, also brings the ecological problem such as environmental pollution, global warming simultaneously.Along with development that is economic and society, industrial and life contaminated water quantity discharged and greenhouse gas emissions, also in cumulative year after year, endanger environment further, reduce efficiency of economic operations.Meanwhile, traditional wastewater processing technology power consumption is large, and greenhouse gas emissions are large, are also unfavorable for environment protection.
Microbiological fuel cell (Microbialfuelcells, MFCs) is the practical new technology of one of electrogenesis and the water treatment developed in recent years.MFCs utilizes microorganism to carry out degradation of organic substances as catalyzer, produces electric energy, and the step achieved from sewage disposal to energy recovery transforms.In addition, it is high that MFCs also has transformation efficiency, wide application range of substrates, and operational condition is gentle, and microorganism can the many merits such as self-replacation as catalyzer, and therefore, it will have limitless application and development prospect.
But at present, the anode efficiency of MFCs is the principal element that restriction MFCs carries out electrogenesis and waste water treatment applications.First, the anode electrogenesis bacterium of most of MFCs is separated to get from active sludge, is several mixt bacterias with synergy, syntrophism.Secondly, the mixt bacteria of separating will be cooked in the MFCs assembled and carry out inoculating to tame its electrogenesis characteristic, and this domestication needs through multiple cycle, and domestication process expends time in very much.Again, the growing environment of electrogenesis mixt bacteria is not quite similar, and under different envrionment conditionss, the kind of dominant bacteria may be different, so affect electricity generation performance and the efficiency of MFCs.Therefore, work out and a kind ofly induce mixing electrogenesis bacteria distribution, the method for growth and the domestication of electrogenesis characteristic and reactor, fundamentally can not only improve the anode efficiency of MFCs, electrogenesis bacteria distribution is easy, time saving and energy saving with domestication process simultaneously, is of great significance the large-scale practical application tool of MFCs.
Summary of the invention
The object of the invention is to, the device of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication is provided, this device can improve the efficiency of anode of microbial fuel cell electrogenesis bacteria distribution, growth and domestication, microbial inoculant after separation domestication can be shortened in anode of microbial fuel cell room the start time of microbiological fuel cell, improve battery circuit and power density output, reduce the internal resistance of cell etc.
In order to achieve the above object, present invention employs following technical scheme:
The device of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication, described device comprises the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 be oppositely arranged, first conductive carbon fibre pole plate 6 is connected with stepper-motor 1, second conductive carbon fibre pole plate 9 is connected with stadimeter 3, and the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 form an electric field loop with direct supply 7 and switch 8, a described electric field loop electric field monitoring device 4 in parallel;
Described first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 are arranged in a magneticfield coil 5, and described magneticfield coil 5 is placed in an airtight transparent vessel 10, and the two ends of described magneticfield coil 5 are connected with magnetic field power supply 2 respectively.
Stepper-motor 1 of the present invention controls the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9, mixt bacteria is seeded on one of them conductive carbon fibre pole plate, by controlling the distance between two conductive carbon fibre pole plates, bacterium grows to another conductive carbon fibre pole plate induction electrogenesis.
Stadimeter 3 of the present invention is for measuring the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9, and the numerical value reflected by stadimeter 3 is long divided by the average body of electrogenesis bacterium, substantially can estimate thalline number on an electrogenesis bacterium chain.
Magnetic field power supply 2 of the present invention and magneticfield coil 5 are in order to produce a stable magnetic field, first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 are placed in magneticfield coil 5, the mixt bacteria of the action of a magnetic field on pole plate produced, by induced by magnetic field electrogenesis bacteria distribution, growth and domestication.
Switch 8 of the present invention controls switching on and off of whole electric field, and direct supply 7 provides electric field to induce separation and the domestication of electrogenesis bacterium for two conductive carbon fibre pole plates, and electric field monitoring device 4 monitors the size and change etc. of current/voltage in electric field.
Another object of the present invention is, provides a kind of method of electromagnetically induced electrogenesis bacteria distribution based on said apparatus, growth and domestication, said method comprising the steps of:
1) mixt bacteria in the middle of anaerobic sludge is seeded on the first conductive carbon fibre pole plate 6, keeps the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 to be 0.5 ~ 1 millimeter;
2) turn on-switch 8, open magnetic field power supply 2 simultaneously and produce stable magnetic field to magneticfield coil 5 energising, the electric current now in electric field monitoring device 4 is 0;
3) under the dual function of Electric and magnetic fields, through the growth of 4 ~ 6 hours, when electrogenesis bacterial growth on first conductive carbon fibre pole plate 6 arrives the second conductive carbon fibre pole plate 9 surface, because electrogenesis bacterium has electroconductibility, electric field loop thus connect, the electric current now in electric field monitoring device 4 is no longer 0;
4) stepper-motor 1 is regulated, the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 is made to be increased to 1 ~ 2 millimeter, now circuit disconnects again, electric current in current monitoring device 4 is 0 again, again through 3 ~ 5 hours, when electrogenesis bacterium on first conductive carbon fibre pole plate 6 regrows arrival second conductive carbon fibre pole plate 9 surface, circuit is connected again;
5) repeating step 3)-4) until the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 is after 4 ~ 8 millimeters, when after circuit ON, disconnect magnetic field and electric field, take off the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9, obtain the electrogenesis bacterium through electromagnetic inducing separation, growth and domestication.
Described electrogenesis bacterium can for bacillus, Klebsiella, Bacillaceae or Rhodopseudomonas can produce in the bacterial classification of electronics one or more.
The electrogenesis microbionation being separated domestication is carried out in the middle of anode of microbial fuel cell room experiment and the test of electrogenesis.
The present invention carries out being separated of electrogenesis bacterium, growth and domestication by magnetic field and the acting in conjunction of electric field, and it has following remarkable advantage relative to prior art: 1) be separated by Electric and magnetic fields electrogenesis bacterium and tame, time-saving and efficiency; 2) by regulating the size of Electric and magnetic fields, specific selection being carried out to the electrogenesis bacterium of some kind, improving the selectivity of strain separating; 3) be separated by this method and in the middle of the electrogenesis microbionation of taming to microbiological fuel cell after, start time shortens, and output rating improves.
Accompanying drawing explanation
Fig. 1 is the structural representation of device of electromagnetically induced electrogenesis bacteria distribution of the present invention, growth and domestication;
Wherein, 1, stepper-motor; 2, magnetic field power supply; 3, stadimeter; 4, electric field monitoring device; 5, magneticfield coil; 6, the first conductive carbon fibre pole plate; 7, direct supply; 8, switch; 9, the second conductive carbon fibre pole plate; 10, transparent airtight container.
Embodiment
With the drawings and specific embodiments, the present invention is further detailed explanation below.
Embodiment 1
As shown in Figure 1, the device of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication, described device comprises the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 be oppositely arranged, first conductive carbon fibre pole plate 6 is connected with stepper-motor 1, second conductive carbon fibre pole plate 9 is connected with stadimeter 3, and the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 form an electric field loop with direct supply 7 and switch 8, a described electric field loop electric field monitoring device 4 in parallel;
Described first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 are arranged in a magneticfield coil 5, and described magneticfield coil 5 is placed in an airtight transparent vessel 10, and the two ends of described magneticfield coil 5 are connected with magnetic field power supply 2 respectively.
Based on a method for the electromagnetically induced electrogenesis bacteria distribution of said apparatus, growth and domestication, said method comprising the steps of:
1) mixt bacteria in the middle of the anaerobic sludge of Beijing sewage work is seeded on the first conductive carbon fibre pole plate 6, keeps the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 to be 0.5 ~ 1 millimeter;
2) turn on-switch 8, open magnetic field power supply 2 simultaneously and produce stable magnetic field to magneticfield coil 5 energising, the electric current now in electric field monitoring device 4 is 0;
3) under the dual function of Electric and magnetic fields, through the growth of 4 ~ 6 hours, when electrogenesis bacterial growth on first conductive carbon fibre pole plate 6 arrives the second conductive carbon fibre pole plate 9 surface, because electrogenesis bacterium has electroconductibility, electric field loop thus connect, the electric current now in electric field monitoring device 4 is no longer 0;
4) stepper-motor 1 is regulated, the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 is made to be increased to 1 ~ 2 millimeter, now circuit disconnects again, electric current in current monitoring device 4 is 0 again, again through 3 ~ 5 hours, when electrogenesis bacterium on first conductive carbon fibre pole plate 6 regrows arrival second conductive carbon fibre pole plate 9 surface, circuit is connected again;
5) repeating step 3)-4) until the distance between the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9 is after 4 ~ 8 millimeters, when after circuit ON, disconnect magnetic field and electric field, take off the first conductive carbon fibre pole plate 6 and the second conductive carbon fibre pole plate 9, obtain the electrogenesis bacterium of the Klebsiella through electromagnetic inducing separation, growth and domestication.
Comparative example 1
Bacterium liquid 20mL embodiment 1 separation being obtained Klebsiella electrogenesis bacterium is inoculated into anode and cathode volume and is in the H type double-chamber microbiological fuel cell of 100mL, external 500 ohmic resistances, with 20mM sodium-acetate for substrate, electrolytic solution is made up of following several material: 0.13g/LKCl, 0.31g/LNH
4cl, 2.93g/LNaH
2pO
4, 4.09g/LNa
2hPO
4, 2.90g/LNaCl.After 30 hours, start battery completes, and after steady running, output voltage is 390 millivolts.And the Klebsiella electrogenesis bacterium that the mixt bacteria in the middle of the anaerobic sludge of Beijing sewage work obtains through traditional separation screening and acclimation method, the result of identical experimental technique is adopted to be: be about 86 hours start time, after steady running, output voltage is 310 millivolts.From above data, apparatus and method of the present invention are used to be separated electrogenesis bacterium and to tame, the electricity generation performance of the battery that can significantly improve.
Claims (6)
1. an electromagnetically induced electrogenesis bacteria distribution, the device of growth and domestication, it is characterized in that, described device comprises the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) that are oppositely arranged, first conductive carbon fibre pole plate (6) is connected with stepper-motor (1), second conductive carbon fibre pole plate (9) is connected with stadimeter (3), and the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) form an electric field loop with direct supply (7) and switch (8), a described electric field loop electric field monitoring device (4) in parallel,
Described first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) are arranged in a magneticfield coil (5), described magneticfield coil (5) is placed in an airtight transparent vessel (10), and the two ends of described magneticfield coil (5) are connected with magnetic field power supply (2) respectively.
2. the device of electromagnetically induced electrogenesis bacteria distribution according to claim 1, growth and domestication, it is characterized in that, described stepper-motor (1) controls the distance between the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9); Described stadimeter (3) is for measuring the distance between the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9).
3. the device of a kind of electromagnetically induced electrogenesis bacteria distribution according to claim 1, growth and domestication, it is characterized in that, described magnetic field power supply (2) and magneticfield coil (5) produce stable magnetic field, for inducing electrogenesis bacteria distribution, growth and domestication.
4. the device of a kind of electromagnetically induced electrogenesis bacteria distribution according to claim 1, growth and domestication, it is characterized in that, described electric field monitoring device (4) is used for measuring the electric current in described electric field loop.
5., based on a method for the electromagnetically induced electrogenesis bacteria distribution of device described in claim 1, growth and domestication, said method comprising the steps of:
1) mixt bacteria in the middle of anaerobic sludge is seeded on the first conductive carbon fibre pole plate (6), keeps the distance between the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) to be 0.5 ~ 1 millimeter;
2) turn on-switch (8), open magnetic field power supply (2) simultaneously and produce stable magnetic field to magneticfield coil (5) energising, the electric current now in electric field monitoring device (4) is 0;
3) under the dual function of Electric and magnetic fields, through the growth of 4 ~ 6 hours, when electrogenesis bacterial growth on first conductive carbon fibre pole plate (6) arrives the second conductive carbon fibre pole plate (9) surface, because electrogenesis bacterium has electroconductibility, electric field loop thus connect, the electric current now in electric field monitoring device (4) is no longer 0;
4) stepper-motor (1) is regulated, the distance between the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) is made to be increased to 1 ~ 2 millimeter, now circuit disconnects again, electric current in current monitoring device (4) is 0 again, again through 3 ~ 5 hours, when electrogenesis bacterium on first conductive carbon fibre pole plate (6) regrows arrival second conductive carbon fibre pole plate (9) surface, circuit is connected again;
5) repeating step 3)-4) until the distance between the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9) is after 4 ~ 8 millimeters, when after circuit ON, disconnect magnetic field and electric field, take off the first conductive carbon fibre pole plate (6) and the second conductive carbon fibre pole plate (9), obtain the electrogenesis bacterium through electromagnetic inducing separation, growth and domestication.
6. method according to claim 5, is characterized in that, described step 5) the electrogenesis bacterium that obtains be Klebsiella can produce in the bacterial classification of electronics one or more.
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| CN104894004B (en) * | 2015-04-17 | 2018-03-23 | 中国科学院过程工程研究所 | The Mixed Microbes and application that a kind of method of the electricity production function bacterium of screening energy degrading chlorophenol, screening obtain |
| CN105296353B (en) * | 2015-12-02 | 2017-10-10 | 沈阳化工研究院有限公司 | A kind of bacterial strain electricity domestication equipment |
| CN106399090B (en) * | 2016-09-07 | 2019-01-22 | 山东大学 | Electromagnetic environment generator and experimental method for microculture test |
| CN108658213B (en) * | 2018-04-19 | 2021-08-03 | 浙江工商大学 | Electrochemical device and method for in-situ acceleration of low-temperature anaerobic microorganism domestication |
| CN109987719B (en) * | 2019-03-20 | 2023-10-27 | 山东师范大学 | Device and method for separating electrogenesis bacteria |
| CN112759067A (en) * | 2021-02-03 | 2021-05-07 | 昆明理工大学 | Method and device for electromagnetic enhanced biological treatment of organic wastewater |
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| CN1949577A (en) * | 2005-10-14 | 2007-04-18 | 中国科学院过程工程研究所 | Bioreactor-direct microbe fuel cell and use thereof |
| CN101570731A (en) * | 2009-03-25 | 2009-11-04 | 新奥科技发展有限公司 | Method for domesticating and separating electricigens by electrochemistry |
| CN101607781A (en) * | 2009-07-17 | 2009-12-23 | 广东省生态环境与土壤研究所 | A kind of microbiological cell device and municipal sludge disposal method |
| CN102277388A (en) * | 2011-06-20 | 2011-12-14 | 中国科学院广州能源研究所 | Coproduction method and device of hydrogen and electricity by organic wastes |
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| US7807303B2 (en) * | 2008-06-30 | 2010-10-05 | Xerox Corporation | Microbial fuel cell and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1949577A (en) * | 2005-10-14 | 2007-04-18 | 中国科学院过程工程研究所 | Bioreactor-direct microbe fuel cell and use thereof |
| CN101570731A (en) * | 2009-03-25 | 2009-11-04 | 新奥科技发展有限公司 | Method for domesticating and separating electricigens by electrochemistry |
| CN101607781A (en) * | 2009-07-17 | 2009-12-23 | 广东省生态环境与土壤研究所 | A kind of microbiological cell device and municipal sludge disposal method |
| CN102277388A (en) * | 2011-06-20 | 2011-12-14 | 中国科学院广州能源研究所 | Coproduction method and device of hydrogen and electricity by organic wastes |
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