CN104894004B - The Mixed Microbes and application that a kind of method of the electricity production function bacterium of screening energy degrading chlorophenol, screening obtain - Google Patents
The Mixed Microbes and application that a kind of method of the electricity production function bacterium of screening energy degrading chlorophenol, screening obtain Download PDFInfo
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- CN104894004B CN104894004B CN201510184879.4A CN201510184879A CN104894004B CN 104894004 B CN104894004 B CN 104894004B CN 201510184879 A CN201510184879 A CN 201510184879A CN 104894004 B CN104894004 B CN 104894004B
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Abstract
The Mixed Microbes obtained the invention discloses a kind of method of the electricity production function bacterium of screening energy degrading chlorophenol, screening and its application.The present invention filters out the electricity production bacterium with degrading chlorophenol performance from existing ocean hydrothermal sedment thing, difficult degradation Chlorophenol is handled in microbial fuel cells system using the bacterium, electric energy is produced by microbial fuel cells system, can be by waste water reclaiming energy.The present invention has the advantages such as operating cost is low, operating condition is convenient, and can effectively filter out purpose function bacterium, and uses it for wastewater treatment.The present invention is to concentrate the screening of degrading chlorophenol electricity production function bacterium, sewage disposal, energy, resource reclaim to be had broad application prospects in integral handling process.
Description
Technical field
The invention belongs to sewage treatment area, and in particular to a kind of side of the electricity production function bacterium of screening energy degrading chlorophenol
The Mixed Microbes and its application that method, screening obtain.
Background technology
Chlorophenols compound is widely used in timber preservative, weeding as important chemical industry Organic Ingredients and intermediate
In the industrial productions such as agent, bactericide, insecticide, dyestuff, chlorophenols compound also can result from incineration of waste as accessory substance, make
In paper pulping bleaching and chlorination of drinking water disinfecting process.Chlorophenols compound is artificial synthesized exogenous material, has stench
And there are good chemical stability and heat endurance, the natural microbial in nature can not secrete corresponding enzyme to degrade this
Class material, cause the extended residual of this kind of compound in the environment.As a kind of Typical Representative of persistent organic pollution,
Chlorophenols compound can be immersed in organism by skin contact or the suction of direct oral cavity mucous membrane, cell is lost activity, promoted
Lesion occurs for nervous system, and it is serious to health and natural environment influence.It is untreated to be completely discharged into water containing Chlorophenol
After body environment, serious pollution is produced to water body and aquatic organism, influences to store the ecological environment security of water body.
The recovery technique research of chlorophenols pollutant effluents is more, and it mainly includes physical chemistry method and biochemical method.Materialization side
Method includes reduction dechlorination, chemical oxidation, charcoal absorption method.Physical chemistry method can increase extra energy consumption simultaneously in processing procedure
Other chemical reagent are introduced, therefore, it is also desirable to carry out follow-up after-treatment.And biochemical method, such as Aerobic biological process and
Anaerobic bio-treated, immobilized microorganism technique and bioelectrochemical system treatment technology, there is relatively economical, substantially without two
The features such as secondary pollution, increasingly draw attention, and be increasingly becoming a kind of environmental improvement skill for generally being received and being approved by people
One of art.Wherein bioelectrochemistry treatment technology, because electrochemical reduction is capable of the toxicity of degradation material, improve giving birth to for material
Thing degradability, there is larger advantage as pretreating process.
Microbiological fuel cell technology is field of environment engineering wastewater treatment means emerging in recent years, and Microbial fuel is electric
Pool technology is to be used as catalyst by microorganism, and the organic substrates in waste water are carried out into catabolism, while by the change in substrate
The final electron acceptor that can be converted into electronics, proton and negative electrode is combined, and completes final course of reaction.Pass through microorganism
Fuel cell system, clean energy resource electric energy can be extracted from organic wastewater.Therefore, microbiological fuel cell technology be can be real
Existing waste water energy.Microbial fuel system can equally turn into a kind of screening and produce electricity bacterium as a biological treatment process
With effective means of the processing containing Chlorophenol.
The content of the invention
The invention aims to filtered out using single chamber air cathode microbial fuel cell with degrading chlorophenol
The electricity production function bacterium of energy, and the bacterium is used for microbiological fuel cell processing and contains Chlorophenol.
Present invention utilizes a kind of device for screening degrading chlorophenol electricity production function bacterium, described device includes lucite
Groove, negative electrode graphite felt electrode and anode graphite felt electrode；The upper end of wherein lucite groove is open, and bottom land seals and electrode is housed
Liquid；Negative electrode graphite felt electrode and anode graphite felt electrode are connected with titanium silk respectively, and are connected and sealed by silicone tube, described
The external circuit of device connects constant resistance.
Preferably, the external circuit of described device connects 510 ohm of constant resistances, and voltage data acquisition system is connected to electricity with titanium silk
Hinder both ends.
The screening technique of electricity production function bacterium provided by the invention with degrading chlorophenol performance includes：
Using marine sediment samples as bacterium source, the air for starting the chlorophenol anolyte that chlorophenol concentration is 20-50mg/L is cloudy
Atomic biological fuel cell, and the constant external resistance between anode and negative electrode is set as 505~510 ohm, and it is steady in output voltage
Increase the chlorophenol concentration of anolyte after fixed to 50~150mg/L, the biomembrane formed on anode graphite felt is to have degraded chlorine
The electricity production bacterium of phenol.
Preferably, the initial concentration of chlorophenol is 50mg/L in the air cathode microbial fuel cell, and the chlorophenol exists
150mg/L is increased to after output voltage stabilization.
A kind of specifically, behaviour using single chamber air cathode microbial fuel cell screening dechlorination electricity production bacterium of the present invention
It is as follows to make method：Chlorophenol anolyte will be contained to be injected into air cathode microbial fuel cell system, and 0.1g ocean is hot
Liquid deposit (Chinese ocean sample the shop ,-S10-TVG6 of NO.21 III) is inoculated in anolyte, under the fixation extrernal resistance of setting, is opened
Dynamic single chamber air cathode microbial fuel cell, monitor the change in concentration and the system of chlorophenol noxious material in the system
Output voltage changes, and when output voltage stabilization operation (output voltage reaches stable within 2-3 cycle), increases in anolyte
Chlorophenol concentration, the electricity production bacterium of resisting high-concentration chlorophenol and energy degrading chlorophenol is further screened, the electricity production bacterium with degrading chlorophenol
It is the electricity production bacterium with degrading chlorophenol performance to be formed on anode graphite felt on stable biomembrane biomembrane.
Preferably, produce electricity bacterium strain composition be：Cupriavidus (75.12%~77.74%),
Brucellaceae (11.1%~13.89%), Brevundimonas (10.14%~13.56%), other trace of bacteria
(1%~2.31%)；Mixed Microbes parachlorphenol clearance is up to 97%, and cell output voltage is up to 600mV.
The single chamber air cathode microbial fuel cell system of the present invention, can filter out the electricity production with degrading chlorophenol performance
Bacterium, it can be used for processing to contain Chlorophenol, and realize the recovery energy of waste water reclaiming containing chlorophenol.
The method of the present invention changes anolyte and rejoins chlorophenol and the startup of sodium acetate substrate after stable biomembrane is formed
The new cycle, while COD concentration and chlorophenol change in concentration are monitored, it is that the bacterium is handling the application containing Chlorophenol.
Compared with prior art, the invention has the advantages that：
1st, ocean hydrothermal sedment thing is used as bacterium source, utilizes the single chamber air cathode microbial fuel electricity of the anolyte containing chlorophenol
Cell system screening degrading chlorophenol electricity production function bacterium, and the bacterium is used to handle and contains Chlorophenol, it can realize and be given up containing chlorophenol
Recycling of water resource recovery energy.
2nd, screening is connected in the present invention and obtains the single chamber air cathode microbial fuel cell of Mixed Microbes to concentration containing chlorophenol
The clearance that the clearance of 150mg/L waste water can reach more than 85%, COD can reach more than 85%.
3rd, the present invention can be effective using the single chamber air cathode microbial fuel cell after being inoculated with degrading chlorophenol function bacterium
Degraded contains Chlorophenol, has notable economy and Significance for Environment.
Brief description of the drawings
Fig. 1 is that air cathode microbial fuel cell screens dechlorinating bacteria and the signal of wastewater treatment containing chlorophenol in embodiment 1
Figure；1 is that single chamber air cathode microbial fuel cell screens strain system in figure, and 2 be voltage data acquisition system, and 3 be organic
Glass groove, 4 be negative electrode graphite felt electrode, and 5 be anode graphite felt electrode, and 6 be the anolyte containing chlorophenol, and 7 and 8 be titanium silk, 9
It is 510 Ohmic resistances for silicone tube, 10.
Fig. 2 is the COD degradation curve map of different chlorophenol concentration batches in embodiment 2；
Fig. 3 is the chlorophenol degradation curve map of different chlorophenol concentration batches in embodiment 3；
Fig. 4 is open circuit and closed circuit lower chlorophenol degradation curve map in embodiment 4；
Fig. 5 is output voltage curve figure under different chlorophenol concentration batches in embodiment 5.
Embodiment
The present invention is described further below in conjunction with accompanying drawing and example, but it is not limited the scope of the present invention.
The system of electricity production bacterium of the screening that the present invention uses with degrading chlorophenol performance, by single chamber air cathode microbial
Fuel cell system 1 and voltage data acquisition system 2 are formed, and voltage data acquisition system 2 passes through outer lead and air cathode
Microbial fuel cells system 1 connects.
Described air cathode microbial fuel cell system 1 by anode graphite felt electrode 5, negative electrode graphite felt electrode 6, have
Machine glass groove 3 is formed；Wherein, the bottom end seal of air cathode microbial fuel cell system 1, upper end are open, lucite cup
Groove 3 fills the anolyte 6 containing chlorophenol.Anode electrode 5, cathode electrode 4 are connected with titanium silk 7,8 respectively, and titanium silk 7 passes through silicone tube 9
Hermetically passing graphite felt electrode 4.The external 510 ohm of constant resistances 10 of battery circuit, 10 liang of termination voltage datas of constant resistance are adopted
Collecting system 2.
Using-the S10-TVG6 of ocean hydrothermal sedment thing NO.21 III as bacterium source, start the 50mg/L of concentration containing chlorophenol, 100mg/
L, 150mg/L single chamber air cathode microbial fuel cell, 510 ohm of the constant external resistance of negative and positive interpolar, monitoring system
Voltage output situation, output voltage reaches stable within 2-3 cycle after output voltage stabilization is run, and increases in anolyte
Chlorophenol concentration, the electricity production bacterium of resisting high-concentration chlorophenol and energy degrading chlorophenol is further screened, the electricity production bacterium with degrading chlorophenol
Biomembrane is formed on anode graphite felt.In two following embodiments, the marine sediment samples are from ocean 21
Flight number, Southwest Pacific Area seabed is collected in, the sampling time is on 07 18th, 2010, during existing preservation depositary institution is entitled
Ocean mineral resources research and development association of state, address is Qingdao City GaoKeYuan Xianxia Ling road 6, and the deposit number of sample is
NO.21Ⅲ-S10-TVG6。
Embodiment one
Using ocean hydrothermal sedment thing as bacterium source, start containing the micro- life of single chamber air cathode that poisonous chlorophenol concentration is 50mg/L
Thing fuel cell, 510 ohm of the constant external resistance of negative and positive interpolar, the voltage output situation of monitoring system, work as output voltage stabilization
Output voltage reaches stable within 2-3 cycle after operation, increases the chlorophenol concentration in anolyte to 120mg/L, further sieves
The electricity production bacterium of resisting high-concentration chlorophenol and energy degrading chlorophenol is selected, the electricity production bacterium with degrading chlorophenol is formed on anode graphite felt
Biomembrane.
COD removal effect in the wastewater treatment process of embodiment two
One of the composite targets of COD COD as the contaminated degree of water body, are needed in wastewater treatment process
The index of emphasis monitoring.Start microbial fuel cells system, run the system of embodiment one at room temperature and investigate system
Running situation.The COD of system degraded situation, is shown in Fig. 2.
As shown in Figure 2, when TCP initial concentrations are 100mg/L, the COD clearances after processing 5 days are up to 82.34%；When
When TCP initial concentrations are 150mg/L, after same processing 5 days, COD clearances are up to 83.85%.Both COD removal effects are close,
Illustrate in the range of the investigation of TCP initial concentrations, the rise of TCP initial concentrations influences smaller on COD removal effects.
The removal effect of chlorophenol in the wastewater treatment process of embodiment three
For Waste Water Treatment containing chlorophenol, it is necessary first to which investigation is gone containing the poisonous chlorophenol in Chlorophenol
Except effect.The method that the present embodiment uses embodiment one, investigates the clearance in the chlorophenol of different reaction density batches respectively,
See Fig. 3.From the figure 3, it may be seen that when initial TCP concentration increases, the residual TCP concentration in solution is also bigger, under TCP clearance
Drop.When initial concentration is 50mg/L, TCP clearance can reach 97.45%.When initial concentration is improved to 150mg/L,
TCP clearance drops to 87.32%.
Open circuit contrasts with closed circuit chlorophenol removal effect in example IV wastewater treatment process
In order to illustrate trickle biological fuel cell dechlorination effect and anaerobic fermentation dechlorination effect.The present embodiment uses embodiment
One method is closed circuit, and the method for external resistance open circuit is laid down with the difference of embodiment one, in the case where other conditions are consistent, respectively
Examination bacterium dechlorination effect, is shown in Fig. 4 under the conditions of MFC opens a way and be closed circuit.It can be drawn from Fig. 4, when TCP initial concentrations are
During 100mg/L, under the conditions of closed circuit, TCP clearance is 66.72%；In the open circuit condition, TCP clearance is
57.43%.TCP clearances under the conditions of closed circuit are higher about 9 percentage points than the TCP clearances under open-circuit condition.Illustrate testing
Under the conditions of, the dechlorination effect of microbiological fuel cell is more excellent than traditional anaerobic fermentation dechlorination effect.
Voltage output effect in the wastewater treatment process of embodiment five
Using voltage data acquisition system, the electric energy captured in microbiological fuel cell chlorophenol degradation system is stored
And boosting.Fig. 5 has monitored in embodiment one voltage output result under the conditions of different chlorophenol concentration of substrate.
It should be noted last that the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, it is above-mentioned
Concentration can be also adjusted as needed, such as initial concentration can change in the range of 20-50mg/L, and follow-up chlorophenol is dense
Degree can also adjust in the range of 50-150mg/L.Although the present invention is described in detail with reference to embodiment, this area
Those of ordinary skill it should be understood that being modified to technical scheme or equivalent substitution, without departure from the present invention
The spirit and scope of technical scheme, it all should cover among scope of the presently claimed invention.
Claims (3)
1. a kind of method using single chamber air cathode microbial fuel cell screening degrading chlorophenol electricity production function bacterium, including：
Using marine sediment samples as bacterium source, the air cathode for starting the chlorophenol anolyte that chlorophenol concentration is 20-50mg/L is micro-
Biological fuel cell, and the constant external resistance between anode and negative electrode is set as 505~510 ohm, and after output voltage stabilization
Increase the chlorophenol concentration of anolyte to 50~150mg/L, the biomembrane formed on anode graphite felt is to have degrading chlorophenol
Produce electricity bacterium.
2. screening obtained Mixed Microbes according to the method for claim 1, the strain composition of the Mixed Microbes includes：
Cupriavidus 75.12%~77.74%, Brucellaceae 11.1%~13.89%, Brevundimonas
10.14%~13.56%.
3. the Mixed Microbes of claim 2 are in application of the degraded containing Chlorophenol.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105154375B (en) * | 2015-10-16 | 2019-01-18 | 安徽农业大学 | One kind is for the degrade remaining microbial inoculum of phenoxy carboxylic acid herbicides and preparation method |
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| CN109019832A (en) * | 2018-10-18 | 2018-12-18 | 农业部环境保护科研监测所 | A kind of microorganism electrochemical processing method of organic chemical polluted water |
| CN110451630B (en) * | 2019-07-29 | 2020-10-23 | 中国科学院过程工程研究所 | Device and method for forming electrochemical auxiliary reinforced biofilm with electricity generation and organic chlorine removal functions |
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| CN104277970A (en) * | 2013-07-01 | 2015-01-14 | 中国科学院过程工程研究所 | Device and method for electromagnetic induction of separation, growth and domestication of electrogenic bacteria |
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| CN104277970A (en) * | 2013-07-01 | 2015-01-14 | 中国科学院过程工程研究所 | Device and method for electromagnetic induction of separation, growth and domestication of electrogenic bacteria |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105154375B (en) * | 2015-10-16 | 2019-01-18 | 安徽农业大学 | One kind is for the degrade remaining microbial inoculum of phenoxy carboxylic acid herbicides and preparation method |
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