CN104894004A - Method for screening electrogenesis function bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application of mixed bacteria - Google Patents
Method for screening electrogenesis function bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and application of mixed bacteria Download PDFInfo
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- CN104894004A CN104894004A CN201510184879.4A CN201510184879A CN104894004A CN 104894004 A CN104894004 A CN 104894004A CN 201510184879 A CN201510184879 A CN 201510184879A CN 104894004 A CN104894004 A CN 104894004A
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Abstract
The invention discloses a method for screening electrogenesis function bacteria capable of degrading chlorophenol, mixed bacteria obtained by screening and the application of the bacteria. The electrogenesis function bacteria with chlorophenol degrading performance are screened from existing sea hydrothermal solution sediment, and the bacteria are used for processing degradation-resistant chlorophenol waste water in a microorganism fuel battery system. Electric energy is generated through the microorganism fuel battery system, and waste water becomes resources and energy. The method has the advantages that the operation cost is low, operation conditions are convenient to achieve, and target function bacteria can be screened effectively and are used for waste water processing. The method enables screening of the chlorophenol degrading electrogenesis function bacteria, sewage treatment and energy and resource recycling to be integrated and has a wide application prospect.
Description
Technical field
The invention belongs to sewage treatment area, be specifically related to a kind of screen can degrading chlorophenol electrogenesis function bacterium method, screen the mixed bacterium that obtains and application thereof.
Background technology
Chlorophenols compound is widely used in the industrial production such as wood preservative, weedicide, sterilant, sterilant, dyestuff as important chemical industry organic raw material and intermediate, and chlorophenols compound also can result from Incineration of waste, paper pulping bleaching and chlorination of drinking water sterilizing process as by product.Chlorophenols compound is the exogenous material of synthetic, there is stench and have good chemical stability and thermostability, the natural microbial of occurring in nature can not secrete corresponding enzyme to this kind of material of degrading, and causes this compounds extended residual in the environment.As a kind of Typical Representative of persistent organic pollutant, chlorophenols compound sucks by skin contact or direct oral cavity mucous membrane and immerses in organism, cell is lost activity, impels neural system generation pathology, its to HUMAN HEALTH and natural environment influence serious.Untreated completely containing after Chlorophenol enters water body environment, serious pollution is produced to water body and aquatic organism, the ecological environment security of impact storage water body.
The recovery technique research of chlorophenols pollutant effluents is more, and it mainly comprises physical chemistry method and biochemical method.Physical chemistry method comprises reduction dechlorination, chemical oxidation, charcoal absorption method.Physical chemistry method can increase extra energy consumption and introduce other chemical reagent in treating processes, therefore, also needs to carry out follow-up secondary treatment.And biochemical method, as Aerobic biological process and anaerobic biological treatment, immobilized microorganism technique and bioelectrochemical system treatment technology, there is relatively economical, substantially there is no the features such as secondary pollution, more and more draw attention, and become one of a kind of environmental improvement technology generally accepted by people and approve gradually.Wherein bioelectrochemistry treatment technology, because electrochemical reduction can the toxicity of degradation material, improves the biodegradable of material, has larger advantage as pretreatment technology.
Microbiological fuel cell technology is the emerging in recent years wastewater treatment means of field of environment engineering, microbiological fuel cell technology is as catalyzer by microorganism, organic substrates in waste water is carried out katabolism, be that the final electron acceptor(EA) of electronics, proton and negative electrode combines simultaneously by the chemical energy in substrate, complete final reaction process.By microbial fuel cells system, clean energy electric energy can be extracted from organic waste water.Therefore, microbiological fuel cell technology to realize waste water energy.Microbial fuel system, as a biological treatment process, can become a kind of effective means of screening electrogenesis bacterium and processing containing Chlorophenol equally.
Summary of the invention
The object of the invention is the electrogenesis function bacterium in order to utilize single chamber air cathode microbial fuel cell to filter out with degrading chlorophenol performance, and this bacterium is used for microbiological fuel cell process containing Chlorophenol.
Present invention utilizes a kind of device screening degrading chlorophenol electrogenesis function bacterium, described device comprises organic glass guide channel, negative electrode graphite felt electrode and anode graphite felt electrode; Wherein the upper end of synthetic glass groove is uncovered, and bottom land seals and electrode solution is housed; Negative electrode graphite felt electrode is connected with titanium silk respectively with anode graphite felt electrode, and is connected and sealed by silicone tube, and the external circuit of described device connects constant resistance.
Preferably, the external circuit of described device connects 510 ohm of constant resistance, and voltage data acquisition system and titanium silk are connected to resistance two ends.
The screening method with the electrogenesis function bacterium of degrading chlorophenol performance provided by the invention comprises:
Using marine sediment samples as bacterium source, start the air cathode microbial fuel cell that chlorophenol concentration is the chlorophenol anolyte of 20-50mg/L, and the constant external resistance set between anode and negative electrode is 505 ~ 510 ohm, and after output voltage stabilization, increasing chlorophenol concentration to the 50 ~ 150mg/L of anolyte, the microbial film that anode graphite felt is formed is the electrogenesis bacterium of degrading chlorophenol.
Preferably, the concentration that in described air cathode microbial fuel cell, chlorophenol is initial is 50mg/L, and described chlorophenol is increased to 150mg/L after output voltage stabilization.
Concrete, a kind of working method utilizing single chamber air cathode microbial fuel cell to screen dechlorination electrogenesis bacterium of the present invention is as follows: will be injected in air cathode microbial fuel cell system containing chlorophenol anolyte, and by ocean hydrothermal sedment thing (the sample shop, Chinese ocean of 0.1g, NO.21 III-S10-TVG6) be inoculated in anolyte, under the fixing extrernal resistance of setting, start single chamber air cathode microbial fuel cell, monitor the change in concentration of the chlorophenol toxic substance in this system and the output voltage change of this system, when output voltage stabilization runs (output voltage reaches stable within 2-3 cycle), increase the chlorophenol concentration in anolyte, further screening resisting high-concentration chlorophenol and can the electrogenesis bacterium of degrading chlorophenol, the electrogenesis bacterium with degrading chlorophenol forms the electrogenesis bacterium this microbial film of stable microbial film being and having degrading chlorophenol performance on anode graphite felt.
Preferably, the bacterial classification of electrogenesis bacterium consists of: Cupriavidus (75.12% ~ 77.74%), Brucellaceae (11.1% ~ 13.89%), Brevundimonas (10.14% ~ 13.56%), other trace of bacterias (1% ~ 2.31%); Mixed bacterium P-Chlorophenol clearance can reach 97%, and cell output voltage can reach 600mV.
Single chamber air cathode microbial fuel cell system of the present invention, can filter out the electrogenesis bacterium with degrading chlorophenol performance, and it can be used for process containing Chlorophenol, and realizes containing chlorophenol waste water reclaiming recovery energy.
Method of the present invention is after formation stabilate film, and replacing anolyte rejoins chlorophenol and sodium-acetate substrate starts the new cycle, monitors COD concentration and chlorophenol change in concentration simultaneously, is this bacterium in the application of process containing Chlorophenol.
Compared with prior art, tool of the present invention has the following advantages:
1, ocean hydrothermal sedment thing is adopted to be bacterium source, utilize the single chamber air cathode microbial fuel cell screening system degrading chlorophenol electrogenesis function bacterium containing chlorophenol anolyte, and by this bacterium for the treatment of containing Chlorophenol, can realize containing chlorophenol waste water reclaiming recovery energy.
2, be connected to the single chamber air cathode microbial fuel cell that screening obtains mixed bacterium in the present invention and can reach more than 85% to the clearance that the clearance containing chlorophenol concentration 150mg/L waste water can reach more than 85%, COD.
3, the single chamber air cathode microbial fuel cell after the present invention utilizes inoculation degrading chlorophenol function bacterium can effectively be degraded containing Chlorophenol, has remarkable economy and environment meaning.
Accompanying drawing explanation
Fig. 1 is that in embodiment 1, air cathode microbial fuel cell screens dechlorinating bacteria and contains chlorophenol wastewater treatment schematic diagram; In figure, 1 is single chamber air cathode microbial fuel cell strain screening system, and 2 is voltage data acquisition system, and 3 is synthetic glass cup groove, 4 is negative electrode graphite felt electrode, and 5 is anode graphite felt electrode, and 6 is the anolyte containing chlorophenol, 7 and 8 are titanium silk, and 9 is silicone tube, and 10 is 510 ohmic resistances.
Fig. 2 is the COD degradation graphic representation of different chlorophenol concentration batch in embodiment 2;
Fig. 3 is the chlorophenol degradation graphic representation of different chlorophenol concentration batch in embodiment 3;
Fig. 4 is open circuit and closed circuit lower chlorophenol degradation graphic representation in embodiment 4;
Fig. 5 is different chlorophenol concentration batch lower output voltage graphic representation in embodiment 5.
Embodiment
Be described further of the present invention below in conjunction with accompanying drawing and example, but it does not limit the scope of the present invention.
The screening that the present invention uses has the system of the electrogenesis bacterium of degrading chlorophenol performance, be made up of single chamber air cathode microbial fuel cell system 1 and voltage data acquisition system 2, voltage data acquisition system 2 is connected with air cathode microbial fuel cell system 1 by outer lead.
Described air cathode microbial fuel cell system 1 is made up of anode graphite felt electrode 5, negative electrode graphite felt electrode 6, synthetic glass cup groove 3; Wherein, air cathode microbial fuel cell system 1 bottom end seal, upper end are uncovered, and synthetic glass cup groove 3 fills the anolyte 6 containing chlorophenol.Anode electrode 5, cathode electrode 4 are connected with titanium silk 7,8 respectively, and titanium silk 7 is by silicone tube 9 hermetically passing graphite felt electrode 4.The external 510 ohm of constant resistance 10 of battery circuit, constant resistance 10 liang of termination voltage data acquisition systems 2.
Using ocean hydrothermal sedment thing NO.21 III-S10-TVG6 as bacterium source, start the single chamber air cathode microbial fuel cell containing chlorophenol concentration 50mg/L, 100mg/L, 150mg/L, constant external resistance between anode and cathode 510 ohm, the voltage of Monitoring systems exports situation, after output voltage stabilization runs, output voltage reaches stable within 2-3 cycle, increase the chlorophenol concentration in anolyte, further screening resisting high-concentration chlorophenol and can the electrogenesis bacterium of degrading chlorophenol, the electrogenesis bacterium with degrading chlorophenol forms microbial film on anode graphite felt.In two following embodiments, described marine sediment samples is for deriving from ocean 21 flight number, be collected in Southwest Pacific Area seabed, sampling time is on 07 18th, 2010, existing preservation depositary institution name is called Chinese ocean mineral resources research and development association, address is No. 6, GaoKeYuan Xianxia Ling road, Qingdao City, and the deposit number of sample is NO.21 III-S10-TVG6.
Embodiment one
Using ocean hydrothermal sedment thing as bacterium source, start and contain the single chamber air cathode microbial fuel cell that poisonous chlorophenol concentration is 50mg/L, constant external resistance between anode and cathode 510 ohm, the voltage of Monitoring systems exports situation, after output voltage stabilization runs, output voltage reaches stable within 2-3 cycle, chlorophenol concentration in increase anolyte is to 120mg/L, further screening resisting high-concentration chlorophenol also can the electrogenesis bacterium of degrading chlorophenol, and the electrogenesis bacterium with degrading chlorophenol forms microbial film on anode graphite felt.
The removal effect of COD in embodiment two wastewater treatment process
Chemical oxygen demand COD, as one of the composite target of the contaminated degree of water body, is the index needing emphasis to monitor in wastewater treatment process.Start microbial fuel cells system, at room temperature run the system of embodiment one and investigate the running condition of system.The degraded situation of the COD of this system, is shown in Fig. 2.
As shown in Figure 2, when TCP starting point concentration is 100mg/L, processes the COD clearance after 5 days and reach 82.34%; When TCP starting point concentration is 150mg/L, same process is after 5 days, and COD clearance reaches 83.85%.Both COD removal effects are close, illustrate within the scope of the investigation of TCP starting point concentration, and TCP starting point concentration raises the impact of COD removal effect less.
The removal effect of chlorophenol in embodiment three wastewater treatment process
For containing for chlorophenol Waste Water Treatment, what first need to investigate is removal effect containing the poisonous chlorophenol in Chlorophenol.The present embodiment adopts the method for embodiment one, investigates the clearance of the chlorophenol at different reaction density batch respectively, sees Fig. 3.As shown in Figure 3, when initial TCP concentration increases, the residual TCP concentration in solution is also larger, and the clearance of TCP declines.When starting point concentration is 50mg/L, the clearance of TCP can reach 97.45%.When starting point concentration is increased to 150mg/L, the clearance of TCP drops to 87.32%.
In embodiment four wastewater treatment process, open circuit and closed circuit chlorophenol removal effect contrast
In order to trickle biofuel cell dechlorination effect and anaerobically fermenting dechlorination effect are described.The present embodiment adopts the method for embodiment one closed circuit, and lays down with embodiment one difference the method that external resistance open a way, under other are consistent, examine or check respectively and to open a way and under closed circuit condition, bacterium dechlorination effect, is shown in Fig. 4 at MFC.Can draw from Fig. 4, when TCP starting point concentration is 100mg/L, under closed circuit condition, the clearance of TCP is 66.72%; In the open circuit condition, the clearance of TCP is 57.43%.TCP clearance under closed circuit condition is than the TCP clearance height under open-circuit condition about 9 percentage points.Illustrate under test conditions, the dechlorination effect of microbiological fuel cell is more excellent than traditional anaerobically fermenting dechlorination effect.
Voltage output effect in embodiment five wastewater treatment process
Utilize voltage data acquisition system, the electric energy of catching in microbiological fuel cell chlorophenol degradation system is carried out storing and boosting.Fig. 5 to have monitored in embodiment one voltage Output rusults under different chlorophenol concentration of substrate condition.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, above-mentioned concentration also can adjust as required, and such as starting point concentration can change in the scope of 20-50mg/L, and follow-up chlorophenol concentration also can adjust in the scope of 50-150mg/L.Although with reference to embodiment to invention has been detailed description, will be understood by those skilled in the art that, modify to technical scheme of the present invention or equivalent replacement, do not depart from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (4)
1. utilize single chamber air cathode microbial fuel cell to screen a method for degrading chlorophenol electrogenesis function bacterium, comprising:
Using marine sediment samples as bacterium source, start the air cathode microbial fuel cell that chlorophenol concentration is the chlorophenol anolyte of 20-50mg/L, and the constant external resistance set between anode and negative electrode is 505 ~ 510 ohm, and after output voltage stabilization, increasing chlorophenol concentration to the 50 ~ 150mg/L of anolyte, the microbial film that anode graphite felt is formed is the electrogenesis bacterium of degrading chlorophenol.
2. method according to claim 1 screens the mixed bacterium obtained.
3. mixed bacterium according to claim 2, is characterized in that, the bacterial classification composition of described mixed bacterium comprises: Cupriavidus 75.12% ~ 77.74%, Brucellaceae 11.1% ~ 13.89%, Brevundimonas 10.14% ~ 13.56%.
4. the mixed bacterium of Claims 2 or 3 is in the application of degraded containing Chlorophenol.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105154375A (en) * | 2015-10-16 | 2015-12-16 | 安徽农业大学 | Microbial inoculant for degrading phenoxy carboxylic acid weedicide residues and preparation method thereof |
| CN109019832A (en) * | 2018-10-18 | 2018-12-18 | 农业部环境保护科研监测所 | A kind of microorganism electrochemical processing method of organic chemical polluted water |
| CN110451630A (en) * | 2019-07-29 | 2019-11-15 | 中国科学院过程工程研究所 | A kind of electrochemistry is assisted and strengthened to have the device and method for producing electricity and removing the formation of organochlorine functional biological film |
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| CN104277970A (en) * | 2013-07-01 | 2015-01-14 | 中国科学院过程工程研究所 | Device and method for electromagnetic induction of separation, growth and domestication of electrogenic bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104277970A (en) * | 2013-07-01 | 2015-01-14 | 中国科学院过程工程研究所 | Device and method for electromagnetic induction of separation, growth and domestication of electrogenic bacteria |
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| LIU H 等: "Production of electricity during wastewater treatment using a single chamber microbial fuel cell", 《ENVIRON SCI TECHNOL》 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154375A (en) * | 2015-10-16 | 2015-12-16 | 安徽农业大学 | Microbial inoculant for degrading phenoxy carboxylic acid weedicide residues and preparation method thereof |
| CN109019832A (en) * | 2018-10-18 | 2018-12-18 | 农业部环境保护科研监测所 | A kind of microorganism electrochemical processing method of organic chemical polluted water |
| CN110451630A (en) * | 2019-07-29 | 2019-11-15 | 中国科学院过程工程研究所 | A kind of electrochemistry is assisted and strengthened to have the device and method for producing electricity and removing the formation of organochlorine functional biological film |
| CN110451630B (en) * | 2019-07-29 | 2020-10-23 | 中国科学院过程工程研究所 | Device and method for forming electrochemical auxiliary reinforced biofilm with electricity generation and organic chlorine removal functions |
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