CN110451630B - Device and method for forming electrochemical auxiliary reinforced biofilm with electricity generation and organic chlorine removal functions - Google Patents
Device and method for forming electrochemical auxiliary reinforced biofilm with electricity generation and organic chlorine removal functions Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention discloses a device and a method for forming a biofilm with the functions of generating electricity and removing organic chlorine through electrochemical auxiliary strengthening. In the device, an anode graphite felt is arranged at the bottom of an electrochemical reaction tank, and a cathode graphite felt is arranged at the upper end of the electrochemical reaction tank; the anode graphite felt is connected with the anode titanium wire, the cathode graphite felt is connected with the cathode titanium wire, and the anode titanium wire and the cathode titanium wire are respectively connected with the external resistor, the voltage data acquisition system and the linear direct current power supply; the external resistor, the voltage data acquisition system and the linear direct-current power supply are connected in parallel; a resistance switch is arranged on a connecting line of the anode titanium wire or the cathode titanium wire and the external resistor; a voltage data acquisition system switch is arranged on a connecting line of the anode titanium wire or the cathode titanium wire and the voltage data acquisition system; a power switch is arranged on a connecting wire of the anode titanium wire or the cathode titanium wire and the linear direct-current power supply; the insulating pipe is sleeved on the anode titanium wire. The invention has the advantages of low operation cost, convenient operation condition and the like.
Description
Technical Field
The invention belongs to the field of sewage treatment, and particularly relates to a device and a method for forming an anode biofilm of a microbial fuel cell with a specific function through electrochemical auxiliary reinforcement.
Background
The organic chlorinated compound is a general name of a series of element organic compounds which take carbon or hydrocarbon as a framework and are combined with chlorine atoms and comprises chlorinated alkane, chlorinated alkene and chlorinated aromatic hydrocarbon. The organic chlorinated compounds have unique physical and chemical properties and are widely applied to the industries of chemical industry, electronics, leather making, pesticides and the like. For example, chlorinated hydrocarbons (dichloromethane, trichloroethylene, tetrachloroethylene, etc.) are widely used in processes of mechanical manufacturing, electronic component cleaning, chemical engineering, etc. as an important organic solvent and product intermediate. In addition, chlorinated hydrocarbons play an important bridge role in organic synthesis. The chlorinated hydrocarbon has active chemical property and can produce substitution and elimination reaction, and the introduction of chlorine atom into the compound can change its molecular performance. Finally, chlorinated compounds have certain toxicity, for example, organic chlorine pesticides are widely applied to agricultural production for preventing and treating plant diseases and insect pests, and the development of agriculture in China is promoted in a certain period. However, chlorinated compounds have certain degradation resistance and toxicity, carbon-chlorine bonds of the chlorinated compounds are very stable to hydrolysis, and the greater the number of chlorine substitutions (functional groups), the greater the resistance to biodegradation and photolysis, which brings about adverse effects and even harm to human life and quality of life while benefiting humans.
The removal of organochlorine contaminants is carried out by a variety of methods, including physical, chemical and biological methods. The physical method can not realize the degradation of chlorinated organic compounds, only transfers pollutants from one phase to another phase, and simultaneously causes secondary pollution, has high cost and is not suitable for practical application. The chemical method has strict conditions and is easy to generate secondary pollution; the biological method is greatly influenced by pH and temperature environmental factors, so the requirements on water quality and environmental conditions are high.
The microbial fuel cell takes microorganisms as a catalyst to decompose and metabolize organic substrates in the wastewater and simultaneously convert chemical energy in the substrates into electrons and protons to be combined with a final electron acceptor of a cathode so as to complete a final reaction process. However, the start-up time of a pure microbial fuel cell is long, a biofilm with an electrogenesis function needs to be formed on the surface of an anode when the microbial fuel cell is started, and the electrogenesis microbes on the anode need to be acclimated to have a certain special function when the microbial fuel cell needs to be subjected to special wastewater treatment. The invention adopts an electrochemical auxiliary method to strengthen the formation of anode biomembranes with specific functions and shorten the starting period of the microbial fuel cell. The formed anode biomembrane is used for constructing a microbial fuel cell to treat the organic chlorine wastewater which is difficult to degrade, and the removal of organic chlorine can be effectively realized.
Disclosure of Invention
The invention aims to provide a device and a method for electrochemically assisting and strengthening the formation of a biomembrane with the functions of generating electricity and removing organic chlorine, which adopt electrochemical assistance to strengthen the formation of the biomembrane with the functions of generating electricity and removing organic chlorine and apply the biomembrane to the treatment of organic chlorine-containing wastewater difficult to degrade.
In order to achieve the purpose, the invention adopts the following technical scheme:
a device for forming a biofilm with the functions of generating electricity and removing organic chlorine through electrochemical auxiliary strengthening comprises an electrochemical reaction tank 1, an anode graphite felt 2, a cathode graphite felt 3, a cathode titanium wire 4, an external resistor 5, a voltage data acquisition system 6, a linear direct current power supply 7, an anode titanium wire 8, an isolation tube 9, a power switch 11, a voltage data acquisition system switch 12 and a resistance switch 13;
the anode graphite felt 2 is arranged at the bottom of the electrochemical reaction tank 1, and the cathode graphite felt 3 is arranged at the upper end of the electrochemical reaction tank 1; the anode graphite felt 2 is connected with an anode titanium wire 8, the cathode graphite felt 3 is connected with a cathode titanium wire 4, the anode titanium wire 8 is respectively connected with an external resistor 5, a voltage data acquisition system 6 and a linear direct current power supply 7, and the cathode titanium wire 4 is respectively connected with the external resistor 5, the voltage data acquisition system 6 and the linear direct current power supply 7; the external resistor 5, the voltage data acquisition system 6 and the linear direct current power supply 7 are connected in parallel;
a resistance switch 13 is arranged on a connecting line of the anode titanium wire 8 or the cathode titanium wire 4 and the external resistor 5;
a voltage data acquisition system switch 12 is arranged on a connecting line of the anode titanium wire 8 or the cathode titanium wire 4 and the voltage data acquisition system 6;
a power switch 11 is arranged on a connecting wire of the anode titanium wire 8 or the cathode titanium wire 4 and the linear direct-current power supply 7;
the isolation pipe 9 is sleeved on the anode titanium wire 8.
Preferably, the electrochemical reaction tank 1 is open at the upper end and sealed at the bottom.
Preferably, the electrochemical reaction tank is made of organic glass, and other materials can be used.
Preferably, the isolation tube is made of plastic, and other materials can be used.
The invention also provides a method for forming the biomembrane with the functions of generating electricity and removing organic chlorine by electrochemical assistance, which comprises the following steps:
1) preparing a bacteria source and an inorganic salt culture medium into a bacteria-containing inorganic salt culture solution, adding the bacteria-containing inorganic salt culture solution into an electrochemical reaction tank, wherein the pH value of a bioelectrochemical system is 6-10, and the temperature is 15-45 ℃;
2) under the condition of a microbial electrolytic cell system, operating for 3-5 days under the condition of an external voltage of 0.1-0.8V, and forming a biological membrane with an electricity generating function on an anode graphite felt;
3) observing the voltage condition of the cell under the condition of a microbial fuel cell system, gradually increasing the concentration of organic chlorine in the culture solution containing the sterile inorganic salt after the voltage is stable, and repeating the steps 2) and 3) until the biofilm formed on the anode graphite felt is acclimated until the biofilm with the functions of generating electricity and removing the organic chlorine is formed.
In the invention, the bacteria source is marine hydrothermal sediment NO.21 III-S10-TVG 6.
In the invention, the organic chlorine is 2,4, 6-trichlorophenol.
According to the preferred embodiment of the invention, the method for strengthening the formation of the biofilm with the functions of generating electricity and removing organic chlorine by electrochemical assistance comprises the following specific steps:
1) measuring the volume of the bioelectrochemical device, connecting a bacteria source and an inorganic salt culture medium into the bioelectrochemical device according to 1/4-1/3, adding 10-20 mM sodium acetate, wherein the pH value of a bioelectrochemical system is 6-10, and the temperature is 15-45 ℃;
2) under a microbial electrolytic cell system, setting an external voltage of 0.1V-0.8V, wherein the running time of the external voltage is 3-5 days;
3) observing the voltage condition of the cell under a microbial fuel cell system, repeating the steps 2) and 3) after the voltage is stable and the concentration of organic chlorine in the inorganic salt culture medium is increased, and acclimating the biomembrane formed on the anode graphite felt until the biomembrane which generates electricity and removes the organic chlorine with certain concentration is formed.
In the invention, when the concentration of the 2,4, 6-trichlorophenol in the wastewater is 10-150 mg/L, the removal rate of the 2,4, 6-trichlorophenol in the water body treated by the device can reach more than 85%, and when the concentration of the 2,4, 6-trichlorophenol in the wastewater is 150-600 mg/L, the removal rate of the 2,4, 6-trichlorophenol in the water body treated by the device can reach more than 45%.
In the invention, marine hydrothermal sediment NO.21 III-S10-TVG 6 is used as a bacteria source, the pH of a bioelectrochemical system is 6-10, the temperature is 15-45 ℃, a power switch 11 is closed, a microbial electrolytic cell system is started, the external voltage is 0.1-0.8V, and the running time is 3-5 days; the power switch 11 is switched off, the voltage data acquisition system switch 12 and the resistance switch 13 are switched on to monitor the voltage output condition of the system, when the output voltage stably runs, the concentration of the organic chlorine-containing compound is increased by 30mg/L, 50mg/L, 150mg/L and 300mg/L in sequence, the power switch 11 is switched on, the external voltage is 0.1V-0.8V, the running time is 3-5 days, and the electrogenesis bacteria which are resistant to the high-concentration chlorine-containing organic compound and can degrade the chlorine-containing organic compound are further screened; and (3) switching off the power switch 11, and switching on the voltage data acquisition system switch 12 and the resistance switch 13 to monitor the voltage output condition of the system, so that a biomembrane with the functions of generating electricity and removing organic chlorine is formed when the output voltage stably runs. And (3) carrying out degradation experiments on trichlorophenol solutions with different temperatures, different initial pH values and different initial concentrations by using a microbial fuel cell system. The temperature is 15-45 ℃, the initial pH is 6-10, the initial concentration of the trichlorophenol solution is 10-600 mg/L, and the bioelectrochemical device can realize efficient degradation of the trichlorophenol.
According to the invention, a certain voltage is applied to the microbial fuel cell, and the property that microorganisms with low isoelectric points (usually 2-5) and negative charges under the condition that the pH value is higher than the isoelectric points is utilized, so that the enrichment of the microorganisms on the positive graphite felt can be effectively realized, the metabolic propagation of the electrogenic microorganisms is promoted, and a biomembrane with the electrogenic function is formed on the graphite felt connected with the positive electrode of the external power supply. Gradually increasing the concentration of chlorinated organic matters, and acclimating a biological membrane formed on the positive graphite felt in a microbial electrolytic cell system. The graphite felt forming the biological membrane is used as an anode to construct the microbial fuel cell to degrade organic chlorine compounds, so that the organic chlorine can be effectively removed. According to the method, the microbial fuel cell with the biomembrane for generating electricity and degrading the 2,4, 6-trichlorophenol is formed, and the 2,4, 6-trichlorophenol is efficiently degraded under the conditions that the pH is 6-10, the temperature is 15-45 ℃ and the concentration of the trichlorophenol is 10-600 mg/L.
Compared with the prior art, the invention has the following advantages:
1. the external voltage is beneficial to the rapid enrichment and formation of the biological film on the graphite felt, and the starting period is shortened.
2. After the formed biological membrane with the functions of generating electricity and removing organic chlorine is formed, the chlorine-containing organic wastewater can be directly degraded under a microbial fuel cell system.
3. The device and the method of the invention are used for domesticating the biomembrane which generates electricity and removes 2,4, 6-trichlorophenol and constructing the microbial fuel cell, the removal rate of 150mg/L trichlorophenol wastewater can reach more than 89%, and the removal rate of 600mg/L trichlorophenol wastewater can reach more than 45%.
Drawings
FIG. 1 is a schematic structural diagram of an apparatus for electrochemically assisting and strengthening biofilm formation with the functions of generating electricity and removing organic chlorine according to the invention;
FIG. 2 is a graph showing the voltage change of the microbial fuel cell under different applied voltage conditions in example 2;
FIG. 3 is a graph of the effect of different temperatures on the degradation of 2,4, 6-trichlorophenol in example 6;
FIG. 4 is a graph of the effect of different initial pH on the degradation of 2,4, 6-trichlorophenol in example 6;
FIG. 5 is a graph of the effect of different initial concentrations of 2,4, 6-trichlorophenol on the degradation of 2,4, 6-trichlorophenol in example 6;
reference numerals:
1. an electrochemical reaction tank; 2. an anode graphite felt; 3. a cathode graphite felt; 4. cathode titanium wire; 5. connecting a resistor externally; 6. a voltage data acquisition system; 7. a linear direct current power supply; 8. anode titanium wire; 9. an isolation tube; 10. a culture solution containing sterile inorganic salt; 11. a power switch; 12. a voltage data acquisition system switch; 13. and (4) resistance switching.
Detailed Description
Any feature disclosed in this specification may be replaced by alternative features serving equivalent or similar purposes, unless expressly stated otherwise. Unless expressly stated otherwise, each feature is only an example of a generic series of equivalent or similar features. The description is only for the purpose of facilitating understanding of the present invention and should not be construed as specifically limiting the present invention.
The invention is described in further detail below with reference to the figures and the detailed description.
Example 1
As shown in fig. 1, the device for electrochemically assisting and strengthening the formation of the biofilm with the functions of generating electricity and removing organic chlorine comprises an electrochemical reaction tank 1, an anode graphite felt 2, a cathode graphite felt 3, a cathode titanium wire 4, an external resistor 5, a voltage data acquisition system 6, a linear direct current power supply 7, an anode titanium wire 8, an isolation pipe 9, a power switch 11, a voltage data acquisition system switch 12 and a resistance switch 13;
the anode graphite felt 2 is arranged at the bottom of the electrochemical reaction tank 1, and the cathode graphite felt 3 is arranged at the upper end of the electrochemical reaction tank 1; the anode graphite felt 2 is connected with an anode titanium wire 8, the cathode graphite felt 3 is connected with a cathode titanium wire 4, the anode titanium wire 8 is respectively connected with an external resistor 5, a voltage data acquisition system 6 and a linear direct current power supply 7, and the cathode titanium wire 4 is respectively connected with the external resistor 5, the voltage data acquisition system 6 and the linear direct current power supply 7; the external resistor 5, the voltage data acquisition system 6 and the linear direct current power supply 7 are connected in parallel;
a resistance switch 13 is arranged on a connecting line of the anode titanium wire 8 or the cathode titanium wire 4 and the external resistor 5;
a voltage data acquisition system switch 12 is arranged on a connecting line of the anode titanium wire 8 or the cathode titanium wire 4 and the voltage data acquisition system 6;
a power switch 11 is arranged on a connecting wire of the anode titanium wire 8 or the cathode titanium wire 4 and the linear direct-current power supply 7;
the isolation pipe 9 is sleeved on the anode titanium wire 8.
The upper end of the electrochemical reaction tank 1 is open, the bottom of the electrochemical reaction tank is sealed, the electrochemical reaction tank is made of organic glass, and the isolation pipe is made of plastic.
The electrochemical reaction tank is internally provided with a culture solution (or wastewater containing organic chlorine) 10 containing bacteria and inorganic salt, and the device is a microbial electrolytic cell when a power switch 11 is closed and a voltage data acquisition system switch 12 and a resistance switch 13 are in an off state, so that the strengthening of a biological membrane is facilitated; when the power switch 11 is in an open state and the voltage data acquisition system switch 12 and the resistance switch 13 are in a closed state, the microbial fuel cell is used for treating the organic chlorine wastewater which is difficult to degrade.
In the following examples, marine hydrothermal sediment NO.21 III-S10-TVG 6 was used as a bacterial source (the bacterial source is a known bacterial source and is disclosed in Chinese patent CN 104894004B), a power switch 11 was closed, a microbial cell system was started, a constant voltage of 0.2V was applied between the two electrodes, and the system was operated for 3 days under the voltage condition; the power switch 11 is switched off, the voltage data acquisition system switch 12 and the resistance switch 13 are switched on to monitor the voltage output condition of the system, when the output voltage stably operates, the concentration of the organic chlorine-containing compound is increased by 30mg/L, 50mg/L, 150mg/L and 300mg/L in sequence, the power switch 11 is switched on, the system operates for 3-5 days under the condition of the voltage of 0.1-0.8V, and the electrogenesis bacteria which can resist the high-concentration chlorine-containing organic compound and can degrade the chlorine-containing organic compound are further screened; and (3) switching off the power switch 11, closing the voltage data acquisition system switch 12 and the resistor 13 to monitor the voltage output condition of the system, and when the output voltage stably runs, forming a biological membrane with the functions of generating electricity and removing organic chlorine. And degrading the organic chlorine-containing wastewater in a microbial fuel cell system by using the formed biological membrane.
Example 2
The bioelectrochemical apparatus of example 1 was used, the volume of the bioelectrochemical apparatus was measured, the bacteria source and the inorganic salt medium were inoculated into the bioelectrochemical apparatus at a volume ratio of 1:3, and 20mM sodium acetate was added. Under the microbial electrolysis cell system, the system is respectively set to operate for 5 days under the condition of 0.1V of external voltage, operate for 4 days under the condition of 0.4V and operate for 3 days under the condition of 0.8V, and after the system operates for corresponding time, the system operates under the microbial fuel cell system and the voltage change condition of the microbial fuel cell is observed. The voltage variation of the microbial fuel cell under different applied voltage conditions is shown in fig. 2.
The result shows that the electrochemical auxiliary reinforcement is beneficial to the rapid enrichment and formation of the biomembrane on the graphite felt, and the starting period of the microbial fuel cell is shortened.
Example 3
The bioelectrochemical apparatus of example 1 was used, the volume of the bioelectrochemical apparatus was measured, the bacteria source and the inorganic salt medium were inoculated into the bioelectrochemical apparatus at a volume ratio of 1:3, and 20mM sodium acetate was added. Under the microbial electrolytic cell system, the applied voltage is set to be 0.1V, and the running time is 5 days. Observing the voltage condition of the cell under a microbial fuel cell system, waiting for the voltage to be stable, improving the concentration of 2,4, 6-trichlorophenol in an inorganic salt culture medium, setting an external voltage of 0.1V under a microbial electrolysis cell system, and setting the running time to be 5 days, domesticating a biological membrane formed on an anode graphite felt, observing the voltage condition of the cell under the microbial fuel cell system, and waiting for the voltage to be stable, namely forming the biological membrane which can generate electricity and remove certain concentration of 2,4, 6-trichlorophenol. The bioelectrochemical device was controlled at a temperature of 25 ℃ and an initial pH of 7. The initial concentration of trichlorophenol was controlled to 10 mg/L.
The result shows that the electrochemical auxiliary reinforcement of the biomembrane with the functions of generating electricity and dechlorinating has better degradation effect on the 2,4, 6-trichlorophenol in a microbial fuel cell system, and the degradation effect can reach more than 90 percent.
Example 4
The bioelectrochemical apparatus of example 1 was used to measure the volume of the bioelectrochemical apparatus, and the bacterial source and the inorganic salt medium were inoculated into the bioelectrochemical apparatus at a volume ratio of 1:3, and 10mM sodium acetate was added. Under the microbial electrolytic cell system, the applied voltage is set to be 0.4V, and the running time is 4 days. Observing the voltage condition of the cell under a microbial fuel cell system, waiting for the voltage to be stable, improving the concentration of 2,4, 6-trichlorophenol in an inorganic salt culture medium, setting an external voltage of 0.4V under a microbial electrolysis cell system, and setting the running time to be 4 days, domesticating a biological membrane formed on an anode graphite felt, observing the voltage condition of the cell under the microbial fuel cell system, and waiting for the voltage to be stable, namely forming the biological membrane which can generate electricity and remove certain concentration of 2,4, 6-trichlorophenol. The bioelectrochemical device was controlled at a temperature of 25 ℃ and an initial pH of 7. The initial concentration of trichlorophenol was controlled to 150 mg/L.
The result shows that the electrochemical auxiliary reinforcement of the biomembrane with the functions of generating electricity and dechlorinating has better degradation effect on the 2,4, 6-trichlorophenol in a microbial fuel cell system, and the degradation effect can reach more than 80%.
Example 5
The bioelectrochemical apparatus of example 1 was used, the volume of the bioelectrochemical apparatus was measured, the bacteria source and the inorganic salt medium were inoculated into the bioelectrochemical apparatus in a volume ratio of 1:4, and 20mM sodium acetate was added. Under the microbial electrolytic cell system, the applied voltage is set to be 0.8V, and the running time is 3 days. Observing the voltage condition of the cell under a microbial fuel cell system, waiting for the voltage to be stable, improving the concentration of 2,4, 6-trichlorophenol in an inorganic salt culture medium, setting an external voltage of 0.8V under a microbial electrolysis cell system, and setting the running time to be 3 days, domesticating a biological membrane formed on an anode graphite felt, observing the voltage condition of the cell under the microbial fuel cell system, and waiting for the voltage to be stable, namely forming the biological membrane which can generate electricity and remove certain concentration of 2,4, 6-trichlorophenol. The bioelectrochemical device was controlled at a temperature of 25 ℃ and an initial pH of 7. The initial concentration of trichlorophenol was controlled to 300 mg/L.
The result shows that the electrochemical auxiliary reinforcement of the biomembrane with the functions of generating electricity and dechlorinating has better degradation effect on the 2,4, 6-trichlorophenol in a microbial fuel cell system, and the degradation effect can reach more than 45 percent.
Example 6
The bioelectrochemical apparatus of example 1 was used, the volume of the bioelectrochemical apparatus was measured, the bacteria source and the inorganic salt medium were inoculated into the bioelectrochemical apparatus at a volume ratio of 1:3, and 20mM sodium acetate was added. Under the microbial electrolytic cell system, the applied voltage is set to be 0.2V, and the running time is 5 days. Observing the voltage condition of the cell under a microbial fuel cell system, waiting for the voltage to be stable, improving the concentration of 2,4, 6-trichlorophenol in an inorganic salt culture medium, setting an external voltage of 0.2V under a microbial electrolysis cell system, and setting the running time to be 5 days, domesticating a biological membrane formed on an anode graphite felt, observing the voltage condition of the cell under the microbial fuel cell system, and waiting for the voltage to be stable, namely forming the biological membrane which can generate electricity and remove certain concentration of 2,4, 6-trichlorophenol. The temperature of the bioelectrochemical device is controlled to be 15-45 ℃, and the initial pH value is 6-10. The initial concentration of the trichlorophenol is controlled to be 30-600 mg/L. The influence of different temperatures, different initial pH conditions and different initial concentrations of trichlorophenol on the degradation of trichlorophenol is respectively examined, and the results are shown in FIGS. 3-5.
As can be seen from FIGS. 3 to 5, for a trichlorophenol solution with an initial concentration of 50mg/L, the degradation rate of trichlorophenol is above 80% when the temperature of the bioelectrochemical device is 15 to 45 ℃ and the initial pH is 6 to 10. When the temperature of the bioelectrochemical device is 25 ℃ and the initial pH value is 7, the removal rate of the trichlorophenol wastewater can reach more than 89% when the initial concentration of the trichlorophenol is lower than 150mg/L, and the removal rate of the trichlorophenol wastewater can reach more than 45% when the initial concentration of the trichlorophenol is 600 mg/L.
The method can be realized by upper and lower limit values and interval values of intervals of process parameters (such as temperature, time and the like), and embodiments are not listed.
Conventional technical knowledge in the art can be used for the details which are not described in the present invention.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (5)
1. The method is characterized in that the device comprises an electrochemical reaction tank (1), an anode graphite felt (2), a cathode graphite felt (3), a cathode titanium wire (4), an external resistor (5), a voltage data acquisition system (6), a linear direct current power supply (7), an anode titanium wire (8), an isolation pipe (9), a power switch (11), a voltage data acquisition system switch (12) and a resistance switch (13);
the anode graphite felt (2) is arranged at the bottom of the electrochemical reaction tank (1), and the cathode graphite felt (3) is arranged at the upper end of the electrochemical reaction tank (1); the anode graphite felt (2) is connected with an anode titanium wire (8), the cathode graphite felt (3) is connected with a cathode titanium wire (4), the anode titanium wire (8) is respectively connected with an external resistor (5), a voltage data acquisition system (6) and a linear direct current power supply (7), and the cathode titanium wire (4) is respectively connected with the external resistor (5), the voltage data acquisition system (6) and the linear direct current power supply (7); the external resistor (5), the voltage data acquisition system (6) and the linear direct current power supply (7) are connected in parallel;
a resistance switch (13) is arranged on a connecting line of the anode titanium wire (8) or the cathode titanium wire (4) and the external resistor (5);
a voltage data acquisition system switch (12) is arranged on a connecting line of the anode titanium wire (8) or the cathode titanium wire (4) and the voltage data acquisition system (6);
a power switch (11) is arranged on a connecting wire of the anode titanium wire (8) or the cathode titanium wire (4) and the linear direct-current power supply (7);
the isolation tube (9) is sleeved on the anode titanium wire (8);
the method comprises the following steps:
1) preparing a bacteria source and an inorganic salt culture medium into a bacteria-containing inorganic salt culture solution, adding the bacteria-containing inorganic salt culture solution into an electrochemical reaction tank, wherein the pH value of a bioelectrochemical system is 6-10, and the temperature is 15-45 ℃;
2) under the condition of a microbial electrolytic cell system, operating for 3-5 days under the condition of an external voltage of 0.1-0.8V to form a biomembrane with an electricity generating function on the anode graphite felt;
3) observing the voltage condition of the cell under the condition of a microbial fuel cell system, gradually increasing the concentration of organic chlorine in the culture solution containing the sterile inorganic salt after the voltage is stable, and repeating the steps 2) and 3) until the biofilm formed on the anode graphite felt is acclimated until the biofilm with the functions of generating electricity and removing the organic chlorine is formed.
2. The method according to claim 1, wherein the electrochemical reaction cell (1) is open at the upper end and sealed at the bottom.
3. The method of claim 1, wherein the bacterial source is from marine hydrothermal sediment No.21 iii-S10-TVG 6.
4. The method of claim 1, wherein the organic chloride is 2,4, 6-trichlorophenol.
5. The method according to claim 1, wherein the bacteria source and the inorganic salt culture medium are prepared into the bacteria-containing inorganic salt culture solution according to the volume ratio of 1: 4-1: 3.
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| CN109680291A (en) * | 2018-12-28 | 2019-04-26 | 同济大学 | Enhance the production hydrogen methods and bioelectrochemistry system for producing hydrogen of bioelectrochemistry |
| CN109950585A (en) * | 2019-01-31 | 2019-06-28 | 华中科技大学 | A method of promoting microbiological fuel cell electricity production and sensing capabilities |
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| CN106630177A (en) * | 2016-10-19 | 2017-05-10 | 太原理工大学 | Method and device for treating coking wastewater and producing hydrogen gas by microbial electrolysis cell |
| CN109680291A (en) * | 2018-12-28 | 2019-04-26 | 同济大学 | Enhance the production hydrogen methods and bioelectrochemistry system for producing hydrogen of bioelectrochemistry |
| CN109950585A (en) * | 2019-01-31 | 2019-06-28 | 华中科技大学 | A method of promoting microbiological fuel cell electricity production and sensing capabilities |
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