CN110451630B - A device and method for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine - Google Patents

Abstract

The invention discloses a device and a method for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine. In the device, the anode graphite felt is placed at the bottom of the electrochemical reaction tank, and the cathode graphite felt is placed at the upper end of the electrochemical reaction tank; the anode graphite felt is connected to the anode titanium wire, the cathode graphite felt is connected to the cathode titanium wire, and the anode titanium felt is connected to the anode titanium wire. The wire and the cathode titanium wire are respectively connected with the external resistance, the voltage data acquisition system and the linear DC power supply; the external resistance, the voltage data acquisition system and the linear DC power supply adopt a parallel structure; the anode titanium wire or the cathode titanium wire is connected with the external resistance The resistance switch is set on the connection line of the anode titanium wire or the cathode titanium wire and the voltage data acquisition system switch is set on the connection line; the power switch is set on the connection line between the anode titanium wire or the cathode titanium wire and the linear DC power supply; The isolation tube is sleeved on the anode titanium wire. The invention has the advantages of low operating cost, convenient operating conditions and the like.

Description

A device and method for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine

technical field

The invention belongs to the field of sewage treatment, and in particular relates to a device and a method for electrochemically assisting the formation of an anode biofilm of a microbial fuel cell with specific functions.

Background technique

Organochlorinated compounds are a class of organic compounds whose hydrogen atoms are replaced by chlorine atoms, and are a general term for a series of elemental organic compounds with carbon or hydrocarbon skeletons combined with chlorine atoms, including chlorinated alkanes, chlorinated alkenes, and chlorinated alkanes. Aromatic hydrocarbons. These organochlorinated compounds have unique physical and chemical properties and are widely used in chemical, electronic, leather, pesticide and other industries. For example, chlorinated hydrocarbons (dichloromethane, trichloroethylene, tetrachloroethylene, etc.), as a class of important organic solvents and product intermediates, are widely used in machinery manufacturing, electronic component cleaning, chemical and chemical processes. Besides, chlorinated hydrocarbons play an important bridge role in organic synthesis. Due to the relatively active chemical properties of chlorinated hydrocarbons, substitution and elimination reactions can occur, and the introduction of chlorine atoms into compounds can change their molecular properties. Finally, chlorinated compounds have certain toxicity. For example, organochlorine pesticides have been widely used in agricultural production to control plant diseases and insect pests, which has promoted the development of my country's agriculture for a certain period of time. However, chlorinated compounds have certain resistance to degradation and toxicity, their carbon-chlorine bonds are very stable to hydrolysis, and the greater the number of chlorine substitutions (functional groups), the greater the resistance to biodegradation and photolysis, which is beneficial to human beings. At the same time, it also brings adverse effects and even harm to human survival and quality of life.

There are many methods for the removal of organochlorine pollutants, including physical methods, chemical methods and biological methods. The physical method cannot achieve the degradation of chlorinated organics, but only transfers the pollutants from one phase to another, and also causes secondary pollution, which is costly and unsuitable for practical applications. Chemical methods have harsh conditions and are prone to secondary pollution; biological methods are greatly affected by pH and temperature environmental factors, so they have higher requirements on water quality and environmental conditions.

Bioelectrochemical system is an emerging wastewater treatment method in the field of environmental engineering in recent years. Microbial fuel cells use microorganisms as catalysts to catabolize organic substrates in wastewater, and at the same time convert chemical energy in substrates into electrons, protons and cathodes. The final electron acceptor binds to complete the final reaction process. However, the start-up time of a simple microbial fuel cell is long, and the start-up of a microbial fuel cell requires the formation of a biofilm with electricity-generating function on the surface of the anode. For microbial fuel cells that require special wastewater treatment, it is also necessary to domesticate the electricity-producing microorganisms on the anode. , so that it has some special function. The invention adopts the electrochemical auxiliary method to strengthen the formation of the anode biofilm with specific functions, and shorten the start-up period of the microbial fuel cell. Using the formed anode biofilm to construct a microbial fuel cell to treat refractory organochlorine wastewater can effectively remove organochlorine.

SUMMARY OF THE INVENTION

The purpose of the present invention is to provide a device and method for electrochemically assisted strengthening of the biofilm formation with functions of generating electricity and removing organic chlorine, using electrochemical assistance to strengthen the formation of biofilms with functions of generating electricity and removing organic chlorine and making its It is applied to the treatment of refractory organic chlorine-containing wastewater.

To achieve the above object, the present invention has adopted the following technical scheme:

A device for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine, the device comprising an electrochemical reaction tank 1, an anode graphite felt 2, a cathode graphite felt 3, a cathode titanium wire 4, an external resistor 5, a voltage Data acquisition system 6, linear DC power supply 7, anode titanium wire 8, isolation tube 9, power switch 11, voltage data acquisition system switch 12 and resistance switch 13;

The anode graphite felt 2 is placed at the bottom of the electrochemical reaction tank 1, and the cathode graphite felt 3 is placed at the upper end of the electrochemical reaction tank 1; the anode graphite felt 2 is connected with the anode titanium wire 8, and the cathode graphite felt The felt 3 is connected to the cathode titanium wire 4, the anode titanium wire 8 is respectively connected to the external resistor 5, the voltage data acquisition system 6 and the linear DC power supply 7, and the cathode titanium wire 4 is respectively connected to the external resistor 5 and the voltage data acquisition system 6. It is connected with the linear DC power supply 7; the external resistor 5, the voltage data acquisition system 6 and the linear DC power supply 7 adopt a parallel structure;

A resistance switch 13 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the external resistor 5;

A voltage data acquisition system switch 12 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the voltage data acquisition system 6;

A power switch 11 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the linear DC power supply 7;

The isolation tube 9 is sleeved on the anode titanium wire 8 .

Preferably, the upper end of the electrochemical reaction tank 1 is open, and the bottom of the tank is sealed.

Preferably, the electrochemical reaction tank is made of plexiglass, and other materials may also be used.

Preferably, the insulating tube is made of plastic, but other materials may also be used.

The present invention also provides a method for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine, the method comprising the following steps:

1) The bacteria source and the inorganic salt medium are prepared into a bacteria-containing inorganic salt culture solution, and the bacteria-containing inorganic salt culture solution is added to an electrochemical reaction tank, the pH of the bioelectrochemical system is 6-10, and the temperature is 15-45°C;

2) Under the conditions of the microbial electrolysis cell system, run for 3 to 5 days under the condition of an applied voltage of 0.1V to 0.8V, and a biofilm with electricity generation function is formed on the anode graphite felt;

3) Under the conditions of the microbial fuel cell system, observe the cell voltage, and when the voltage is stable, gradually increase the organic chlorine concentration in the bacterial-containing inorganic salt culture solution, repeat steps 2) and 3), and carry out the biofilm formed on the anode graphite felt. acclimatized until the formation of biofilms capable of generating electricity and removing organochlorine.

In the present invention, the bacterial source is marine hydrothermal sediment NO.21III-S10-TVG6.

In the present invention, the organic chlorine is 2,4,6-trichlorophenol.

According to a preferred embodiment of the present invention, a method for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine, the specific steps are as follows:

1) Measure the volume of the bioelectrochemical device, connect the bacterial source and inorganic salt medium to the bioelectrochemical device at 1/4 to 1/3, and add 10 to 20 mM sodium acetate, and the pH of the bioelectrochemical system is 6 to 10. The temperature is 15～45℃;

2) Under the microbial electrolysis cell system, the applied voltage is set to 0.1V-0.8V, and the operating time of the applied voltage is 3-5 days;

3) The cell voltage is observed under the microbial fuel cell system. After the voltage is stabilized, the concentration of organic chlorine in the inorganic salt medium is increased, and steps 2) and 3) are repeated to domesticate the biofilm formed on the positive electrode graphite felt until a Biofilms that generate electricity and remove certain concentrations of organic chlorine.

In the present invention, when the concentration of 2,4,6-trichlorophenol in the wastewater is 10-150 mg/L, the removal rate of 2,4,6-trichlorophenol in the water body treated by the device of the present invention can reach More than 85%, when the concentration of 2,4,6-trichlorophenol in the wastewater is 150-600 mg/L, the removal rate of 2,4,6-trichlorophenol in the water body treated by the device of the present invention can be improved. more than 45%.

In the present invention, the marine hydrothermal sediment NO.21III-S10-TVG6 is used as the bacterial source, the pH of the bioelectrochemical system is 6-10, the temperature is 15-45°C, the power switch 11 is closed, and the microbial electrolysis cell system is activated, The applied voltage is 0.1V ~ 0.8V, and the running time is 3 ~ 5 days; disconnect the power switch 11, close the voltage data acquisition system switch 12 and the resistance switch 13 to monitor the voltage output of the system, and increase the organic content in turn when the output voltage is stable. The concentration of chlorine compounds is 30mg/L, 50mg/L, 150mg/L, 300mg/L, the power switch 11 is closed, the applied voltage is 0.1V~0.8V, and the running time is 3~5 days. The electricity-producing bacteria that can degrade the chlorine-containing organic compound; disconnect the power switch 11, close the voltage data acquisition system switch 12 and the resistance switch 13 to monitor the voltage output of the system. functional biofilm. The microbial fuel cell system was used to degrade trichlorophenol solutions with different temperatures, different initial pH and different initial concentrations. It is shown that when the temperature is 15-45 ℃, the initial pH is 6-10, and the initial concentration of trichlorophenol solution is 10-600 mg/L, the bioelectrochemical device can achieve high-efficiency degradation of p-trichlorophenol.

In the present invention, by applying a certain voltage to the microbial fuel cell, the microorganisms have a low isoelectric point (usually 2 to 5) and are negatively charged under the condition that the pH is higher than the isoelectric point, so that the microorganisms can effectively realize the positive electrode graphite felt. The enrichment on the surface promotes the metabolic reproduction of electricity-producing microorganisms, thereby forming a biofilm with electricity-producing function on the graphite felt connected to the positive electrode of the external power supply. The concentration of chlorinated organics was gradually increased, and the biofilm formed on the cathode graphite felt was acclimated in the microbial electrolysis cell system. The organic chlorine-containing compounds can be effectively removed by constructing a microbial fuel cell using the graphite felt forming the biofilm as the anode to degrade the organic chlorine-containing compounds. According to the above method, a microbial fuel cell with a biofilm that generates electricity and degrades 2,4,6-trichlorophenol is formed. Under the condition of /L, the efficient degradation of 2,4,6-trichlorophenol was achieved.

Compared with the prior art, the present invention has the following advantages:

1. The applied voltage is conducive to the rapid enrichment and formation of biofilms on the graphite felt and shortens the start-up period.

2. After the formed biofilm with the functions of generating electricity and removing organic chlorine, the chlorine-containing organic wastewater can be degraded directly under the microbial fuel cell system.

3. Using the device and method of the present invention to domesticate a biofilm with electricity generation and removal of 2,4,6-trichlorophenol and construct a microbial fuel cell, the removal rate of 150 mg/L trichlorophenol wastewater can reach more than 89% , the removal rate of 600mg/L trichlorophenol wastewater can reach more than 45%.

Description of drawings

1 is a schematic structural diagram of the device of the present invention for electrochemically assisted strengthening of the biofilm formation with the functions of generating electricity and removing organic chlorine;

Fig. 2 is the voltage change situation of microbial fuel cell under different applied voltage conditions in Example 2;

3 is a graph showing the effect of different temperatures on the degradation of 2,4,6-trichlorophenol in Example 6;

4 is a graph showing the effect of different initial pH on the degradation of 2,4,6-trichlorophenol in Example 6;

Figure 5 is a graph showing the effect of different initial concentrations of 2,4,6-trichlorophenol on the degradation of 2,4,6-trichlorophenol in Example 6;

Reference number:

1. Electrochemical reaction tank; 2. Anode graphite felt; 3. Cathode graphite felt; 4. Cathode titanium wire; 5. External resistor; 6. Voltage data acquisition system; 7. Linear DC power supply; 8. Anode titanium wire; 9 , isolation tube; 10, bacteria-containing inorganic salt culture solution; 11, power switch; 12, voltage data acquisition system switch; 13, resistance switch.

Detailed ways

Any feature disclosed in this specification, unless expressly stated otherwise, may be replaced by other equivalent or alternative features serving a similar purpose. Unless stated otherwise, each feature is only one example of a series of equivalent or similar features. The description is only for helping understanding of the present invention and should not be regarded as a specific limitation of the present invention.

The present invention will be described in further detail below with the accompanying drawings and specific embodiments.

Example 1

As shown in Figure 1, a device for electrochemically assisted strengthening of biofilm formation with functions of generating electricity and removing organic chlorine, the device comprises an electrochemical reaction tank 1, an anode graphite felt 2, a cathode graphite felt 3, and a cathode titanium wire 4 , external resistor 5, voltage data acquisition system 6, linear DC power supply 7, anode titanium wire 8, isolation tube 9, power switch 11, voltage data acquisition system switch 12 and resistance switch 13;

The anode graphite felt 2 is placed at the bottom of the electrochemical reaction tank 1, and the cathode graphite felt 3 is placed at the upper end of the electrochemical reaction tank 1; the anode graphite felt 2 is connected with the anode titanium wire 8, and the cathode graphite felt The felt 3 is connected to the cathode titanium wire 4, the anode titanium wire 8 is respectively connected to the external resistor 5, the voltage data acquisition system 6 and the linear DC power supply 7, and the cathode titanium wire 4 is respectively connected to the external resistor 5 and the voltage data acquisition system 6. It is connected with the linear DC power supply 7; the external resistor 5, the voltage data acquisition system 6 and the linear DC power supply 7 adopt a parallel structure;

A resistance switch 13 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the external resistor 5;

A voltage data acquisition system switch 12 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the voltage data acquisition system 6;

A power switch 11 is provided on the connection line between the anode titanium wire 8 or the cathode titanium wire 4 and the linear DC power supply 7;

The isolation tube 9 is sleeved on the anode titanium wire 8 .

The upper end of the electrochemical reaction tank 1 is open, the bottom of the tank is sealed, the electrochemical reaction tank is made of plexiglass, and the isolation tube is made of plastic.

The electrochemical reaction tank of the present invention is equipped with a bacterial-containing inorganic salt culture solution (or waste water containing organic chlorine) 10. When the power switch 11 of the present invention is closed and the voltage data acquisition system switch 12 and the resistance switch 13 are in an open state, the microorganisms are The electrolytic cell is beneficial to the strengthening of the biofilm; when the power switch 11 is turned off and the voltage data acquisition system switch 12 and the resistance switch 13 are turned on, it is a microbial fuel cell, which is used to treat the refractory organochlorine wastewater.

In the following examples, the marine hydrothermal sediment NO.21III-S10-TVG6 is used as the bacterial source (this bacterial source is a known bacterial source, which has been disclosed in Chinese patent CN104894004B), the power switch 11 is closed, and the microbial electrolysis cell system is activated. , the constant voltage between the two poles is 0.2V, and it will run for 3 days under this voltage condition; disconnect the power switch 11, close the voltage data acquisition system switch 12 and the resistance switch 13 to monitor the voltage output of the system, and increase in turn when the output voltage runs steadily. The concentration of organic chlorine-containing compounds is 30mg/L, 50mg/L, 150mg/L, 300mg/L, close the power switch 11, and run for 3-5 days under the condition of voltage 0.1-0.8V, and further screen the resistance to high-concentration chlorine-containing organic compounds and The electricity-producing bacteria that can degrade the chlorine-containing organic compound; turn off the power switch 11, close the voltage data acquisition system switch 12 and the resistor 13 to monitor the voltage output of the system, when the output voltage runs stably, it forms a power generation and removal of organic chlorine. functional biofilm. The formed biofilm is used to degrade organic chlorine-containing wastewater in a microbial fuel cell system.

Example 2

The bioelectrochemical device of Example 1 was used, the volume of the bioelectrochemical device was measured, the bacterial source and the inorganic salt medium were connected to the bioelectrochemical device in a volume ratio of 1:3, and 20 mM sodium acetate was added. Under the microbial electrolysis cell system, set the applied voltage to run for 5 days under the condition of 0.1V, 4 days under the condition of 0.4V, and 3 days under the condition of 0.8V. Changes in battery voltage. The voltage changes of the microbial fuel cell under different applied voltage conditions are shown in Figure 2.

The results show that electrochemical-assisted strengthening is beneficial to the rapid enrichment and formation of biofilms on graphite felt and shortens the start-up period of microbial fuel cells.

Example 3

The bioelectrochemical device of Example 1 was used, the volume of the bioelectrochemical device was measured, the bacterial source and the inorganic salt medium were connected to the bioelectrochemical device in a volume ratio of 1:3, and 20 mM sodium acetate was added. Under the microbial electrolysis cell system, the applied voltage was set to 0.1V, and the running time was 5 days. The cell voltage was observed under the microbial fuel cell system. After the voltage was stable, the concentration of 2,4,6-trichlorophenol in the inorganic salt medium was increased. Under the microbial electrolysis cell system, the applied voltage was set to 0.1V, and the running time was 5 days. , domesticate the biofilm formed on the cathode graphite felt, observe the cell voltage under the microbial fuel cell system, and when the voltage stabilizes, a biofilm with electricity generation and removal of a certain concentration of 2,4,6-trichlorophenol is formed. The bioelectrochemical device was controlled at a temperature of 25 °C and an initial pH of 7. The initial concentration of trichlorophenol was controlled at 10 mg/L.

The results show that electrochemically assisted biofilms with the functions of electricity generation and dechlorination have a good degradation effect on 2,4,6-trichlorophenol in the microbial fuel cell system, and the degradation effect can reach more than 90%.

Example 4

The bioelectrochemical device of Example 1 was used, the volume of the bioelectrochemical device was measured, the bacterial source and the inorganic salt medium were connected to the bioelectrochemical device in a volume ratio of 1:3, and 10 mM sodium acetate was added. Under the microbial electrolysis cell system, the applied voltage was set to 0.4V, and the running time was 4 days. The cell voltage was observed under the microbial fuel cell system. After the voltage was stable, the concentration of 2,4,6-trichlorophenol in the inorganic salt medium was increased. Under the microbial electrolysis cell system, the applied voltage was set to 0.4V, and the running time was 4 days. , domesticate the biofilm formed on the cathode graphite felt, observe the cell voltage under the microbial fuel cell system, and when the voltage stabilizes, a biofilm with electricity generation and removal of a certain concentration of 2,4,6-trichlorophenol is formed. The bioelectrochemical device was controlled at a temperature of 25 °C and an initial pH of 7. The initial concentration of trichlorophenol was controlled at 150 mg/L.

The results show that electrochemically assisted biofilm with the functions of electricity generation and dechlorination has a good degradation effect on 2,4,6-trichlorophenol in the microbial fuel cell system, and the degradation effect can reach more than 80%.

Example 5

The bioelectrochemical device of Example 1 was used, the volume of the bioelectrochemical device was measured, the bacterial source and the inorganic salt medium were connected to the bioelectrochemical device in a volume ratio of 1:4, and 20 mM sodium acetate was added. Under the microbial electrolysis cell system, the applied voltage was set to 0.8V, and the running time was 3 days. The cell voltage was observed under the microbial fuel cell system. After the voltage was stable, the concentration of 2,4,6-trichlorophenol in the inorganic salt medium was increased. Under the microbial electrolysis cell system, the applied voltage was set to 0.8V, and the running time was 3 days. , domesticate the biofilm formed on the cathode graphite felt, observe the cell voltage under the microbial fuel cell system, and when the voltage stabilizes, a biofilm with electricity generation and removal of a certain concentration of 2,4,6-trichlorophenol is formed. The bioelectrochemical device was controlled at a temperature of 25 °C and an initial pH of 7. The initial concentration of trichlorophenol was controlled at 300 mg/L.

The results show that electrochemically assisted biofilms with the functions of electricity generation and dechlorination have a good degradation effect on 2,4,6-trichlorophenol in the microbial fuel cell system, and the degradation effect can reach more than 45%.

Example 6

The bioelectrochemical device of Example 1 was used, the volume of the bioelectrochemical device was measured, the bacterial source and the inorganic salt medium were connected to the bioelectrochemical device in a volume ratio of 1:3, and 20 mM sodium acetate was added. Under the microbial electrolysis cell system, the applied voltage was set to 0.2V, and the running time was 5 days. The cell voltage was observed in the microbial fuel cell system. After the voltage was stable, the concentration of 2,4,6-trichlorophenol in the inorganic salt medium was increased. In the microbial electrolysis cell system, the applied voltage was set to 0.2V, and the running time was 5 days. , domesticate the biofilm formed on the cathode graphite felt, observe the cell voltage under the microbial fuel cell system, and when the voltage stabilizes, a biofilm with electricity generation and removal of a certain concentration of 2,4,6-trichlorophenol is formed. The temperature of the bioelectrochemical device was controlled to be 15-45 °C, and the initial pH was 6-10. The initial concentration of trichlorophenol is controlled to be 30-600 mg/L. The effects of different temperatures, different initial pH conditions, and different initial concentrations of trichlorophenol on the degradation of trichlorophenol were investigated, and the results are shown in Figure 3-5.

As can be seen from Figure 3-5, for the trichlorophenol solution with an initial concentration of 50 mg/L, when the temperature of the bioelectrochemical device is 15-45 °C and the initial pH is 6-10, the degradation rates of p-trichlorophenol are all in the more than 80%. When the temperature of the bioelectrochemical device is 25℃ and the initial pH is 7, when the initial concentration of trichlorophenol is lower than 150mg/L, the removal rate of trichlorophenol wastewater can reach more than 89%, and when the initial concentration of trichlorophenol is 600mg/L The removal rate of L trichlorophenol wastewater can reach more than 45%.

The process parameters (such as temperature, time, etc.) of the present invention can implement the method by setting the upper and lower limits of the interval and the interval value, and the embodiments are not listed one by one here.

For the content not described in detail in the present invention, conventional technical knowledge in the field can be used.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the embodiments, those of ordinary skill in the art should understand that any modification or equivalent replacement of the technical solutions of the present invention will not depart from the spirit and scope of the technical solutions of the present invention, and should be included in the present invention. within the scope of the claims.

Claims (5)

1. a kind of electrochemically assisted strengthening has the method for generating electricity and removing organic chlorine function biofilm, it is characterized in that, described method adopts following device to form biofilm with electricity generating and removing organic chlorine function, and described device comprises electrochemical Reaction tank (1), anode graphite felt (2), cathode graphite felt (3), cathode titanium wire (4), external resistor (5), voltage data acquisition system (6), linear DC power supply (7), anode titanium wire (8), isolation tube (9), power switch (11), voltage data acquisition system switch (12) and resistance switch (13); The anode graphite felt (2) is placed at the bottom of the electrochemical reaction tank (1), and the cathode graphite felt (3) is placed at the upper end of the electrochemical reaction tank (1); the anode graphite felt (2) and the The anode titanium wire (8) is connected, the cathode graphite felt (3) is connected with the cathode titanium wire (4), and the anode titanium wire (8) is respectively connected with an external resistor (5), a voltage data acquisition system (6) and a linear A DC power supply (7) is connected, and the cathode titanium wire (4) is respectively connected with an external resistor (5), a voltage data acquisition system (6) and a linear DC power supply (7); the external resistor (5), the voltage data acquisition system ( 6) A parallel structure is adopted between the three of the linear DC power supply (7); A resistance switch (13) is arranged on the connection line between the anode titanium wire (8) or the cathode titanium wire (4) and the external resistor (5); A voltage data acquisition system switch (12) is provided on the connection line between the anode titanium wire (8) or the cathode titanium wire (4) and the voltage data acquisition system (6); A power switch (11) is provided on the connection line between the anode titanium wire (8) or the cathode titanium wire (4) and the linear DC power supply (7); The isolation tube (9) is sleeved on the anode titanium wire (8); The method includes the following steps: 1) The bacteria source and the inorganic salt medium are prepared into a bacteria-containing inorganic salt culture solution, the bacteria-containing inorganic salt culture solution is added to the electrochemical reaction tank, the pH of the bioelectrochemical system is 6~10, and the temperature is 15~45℃; 2) Under the condition of microbial electrolysis cell system, under the condition of applied voltage of 0.1V~0.8V for 3~5 days, a biofilm with electricity generation function is formed on the anode graphite felt; 3) Under the conditions of the microbial fuel cell system, observe the voltage of the cell, and after the voltage is stable, gradually increase the concentration of organic chlorine in the bacterial-containing inorganic salt culture solution, repeat steps 2) and 3), and carry out the biofilm formed on the anode graphite felt. acclimatized until the formation of biofilms capable of generating electricity and removing organochlorine. 2 . The method according to claim 1 , wherein the upper end of the electrochemical reaction tank ( 1 ) is open, and the bottom of the tank is sealed. 3 . 3 . The method according to claim 1 , wherein the bacterial source is obtained from marine hydrothermal sediment NO.21III-S10-TVG6. 4 . 4. The method according to claim 1, wherein the organic chlorine is 2,4,6-trichlorophenol. 5 . The method according to claim 1 , wherein the bacteria source and the inorganic salt medium are prepared in a volume ratio of 1:4 to 1:3 to prepare a bacteria-containing inorganic salt culture solution. 6 .

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