CN109950585A - A method of promoting microbiological fuel cell electricity production and sensing capabilities - Google Patents
A method of promoting microbiological fuel cell electricity production and sensing capabilities Download PDFInfo
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- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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Abstract
The invention belongs to microorganism electrochemicals and bio-sensing field, more particularly, to a kind of promotion microbiological fuel cell electricity production and the method for sensing capabilities.In low temperature environment, the anode biomembrane of microbiological fuel cell is tamed, to the microbiological fuel cell output voltage stabilization, obtain the successful microbiological fuel cell sensor of domestication by low temperature, the anode of its microbiological fuel cell for obtaining specific structure by domestication by low temperature produces electricity flora and falls, and promotes the electricity generation ability and sensing capabilities of the microbiological fuel cell sensor.For the method for domestication by low temperature regulating and controlling microbial fuel cell biology community structure proposed by the present invention compared to the fuel cell that room temperature starts, electricity generation performance improves 12.5%-75%, and sensing sensitivity about promotes 2-3 times.
Description
Technical field
The invention belongs to microorganism electrochemicals and bio-sensing field, more particularly, to a kind of promotion Microbial fuel
The method of battery electricity production and sensing capabilities.
Background technique
China's water environment pollution problem is serious, and all kinds of heavy metals and organic poison pass through the approach such as industrial wastewater to natural ring
It discharges in border, causes serious harm to water body.So be badly in need of it is a kind of can to the sensor that water quality is continuously monitored on-line, as
Sewage front end early warning means, provide safeguard for water environment.
Microbiological fuel cell is a kind of using electroactive microorganism as catalyst, by organic matter degradation in sewage and is turned
It is changed to the device of electric energy.Nowadays, the application study in relation to microbiological fuel cell has been directed to Treatment of Sludge, waste water produces electricity, is miniature
The multiple fields such as power supply and biosensor.As one of research hotspot, the bio-sensing based on microbiological fuel cell
Device in terms of water quality monitoring because, compared with significant advantage possessed by conventional method, expanding many related fields scholars all to this and grinding
Study carefully, and obtains certain achievement.
Bio-toxicity sensor based on microbiological fuel cell, using anode electricity production bacterium as Sensing elements, having in water body
Noxious material can inhibit the metabolism and bioactivity of microorganism, so that electricity production voltage be made to die-off, realize the early warning of noxious material
Sensing.It is swift in response, and structure is simple, and low in cost.The electroactive biomembrane of anode is microbiological fuel cell electricity production
Key component, while being also the recognition component of sensing, therefore the characteristic of the electroactive biomembrane of anode can be to microbiological fuel cell
Electricity generation performance and sensing capabilities make a significant impact.
However, the microbiological fuel cell sensor (MFC sensor) of the prior art is made using electroactive biomembrane
To sense recognition component, since the metabolism of microorganism will receive the influence of outside environmental elements (temperature etc.), in low temperature or
Under the extreme conditions such as person's high temperature, microbial activity is suppressed, and electricity generation ability substantially reduces, and MFC sensor is logical at present
The variation of electric signal is crossed to carry out toxicity sensing, therefore sensing capability can be lost, can only have sensing in lesser temperature range
Effect.In order to improve its anti-interference to external environment, increases its Application in Sensing range, have researcher to electroactive biology
Film is coated, although improving the repellence to external environment, also improves the resistivity to toxicant simultaneously,
Therefore its sensing capabilities can decrease.
Summary of the invention
Aiming at the above defects or improvement requirements of the prior art, the present invention provides a kind of productions of promotion microbiological fuel cell
The method of electricity and sensing capabilities, is carried out tame and docile by the anode biomembrane under cryogenic to microbiological fuel cell sensor
Change, experiment finds the anode biomembrane tamed of this specific range of temperatures not only electricity generation ability and to having at low ambient temperatures
The sensing capabilities of malicious heavy metal are promoted, and the sensor after domestication by low temperature under room temperature working environment its electricity generation ability and
Sensing capabilities also greatly promote, and domestication by low temperature increases the Application in Sensing range of MFC sensor, thus solve the micro- of the prior art
The technical problem that biological fuel cell sensor senses performance is bad and operating temperature range is relatively narrow.
To achieve the above object, according to one aspect of the present invention, a kind of promotion microbiological fuel cell electricity production is provided
And the method for sensing capabilities tames the anode biomembrane of microbiological fuel cell, in low temperature environment to the microorganism
Fuel cell output voltage is stablized, i.e. the acquisition successful microbiological fuel cell sensor of domestication by low temperature, passes through domestication by low temperature
The anode electricity production flora for obtaining the microbiological fuel cell of specific structure is fallen, and promotes the electricity production of the microbiological fuel cell sensor
Ability and sensing capabilities;The low temperature environment actual temp range is not higher than 12 DEG C.
Preferably, the method, includes the following steps:
(1) building of microbiological fuel cell sensor: building microbiological fuel cell sensor, the sensor include string
The anode and cathode of connection;
(2) inoculation of microbiological fuel cell sensor: using the matrix solution containing microorganism to the sun of the sensor
Pole biomembrane is inoculated with, and the matrix solution includes sewage;
(3) microbiological fuel cell sensor anode biofilms domestication: at low ambient temperatures to the microbiological fuel cell
The anode biomembrane of sensor is tamed, and certain interval of time replaces matrix solution, to the microbiological fuel cell sensor
Output voltage stabilization, i.e. the acquisition successful microbiological fuel cell sensor of domestication by low temperature.
Preferably, the low temperature environment actual temp range is 8-12 DEG C.
Preferably, the anode is graphite plate, and the cathode is air cathode, and cathode material on stainless (steel) wire by mixing
Active carbon and carbon black are made.
Preferably, the sewage is municipal wastewater.
Preferably, the matrix solution further includes buffer solution and sodium acetate solution.
Preferably, the method, further comprises the steps of:
(4) it microbiological fuel cell heavy metal toxicity sensing testing: is passed to the successful microbiological fuel cell of domestication by low temperature
The heavy metal ion solution of various concentration is added in sensor, by observing sensor output voltage variation, its biography of analysis test
Perceptual energy.
In general, through the invention it is contemplated above technical scheme is compared with the prior art, can obtain down and show
Beneficial effect:
(1) a kind of method that temperature regulation promotes microbiological fuel cell chemical property proposed by the present invention, is utilized
Biological community structure is affected by temperature biggish speciality, changes anode of microbial fuel cell electricity production by control acclimation temperature
Bacterium structure of community selects and optimizes acclimation temperature, so as to improve its chemical property.
(2) present invention by MFC sensor anode biomembrane using the matrix solution containing specified microorganisms type into
Then row inoculation carries out domestication by low temperature in specific low temperature range, obtain specific anode electricity production flora by domestication by low temperature and fall knot
Structure is not only existed compared with the MFC sensor tamed under traditional normal temperature condition by the MFC sensor that this means is tamed
There are better electricity generation ability and sensing capabilities, and its electricity production and sensing capabilities under room temperature working environment under low temperature environment
It greatly promotes, illustrates that domestication by low temperature means have widened the temperature limit of the sensor.
(3) method of present invention promotion MFC sensor chemical property is easy to operate, low in cost, is improving its electricity production
While performance, sensing capabilities are enhanced, are a kind of environmentally protective and sustainable sewage treatment and monitoring water quality on line skill
Art.
(4) method of domestication by low temperature regulating and controlling microbial fuel cell biology community structure proposed by the present invention is compared to room temperature
The fuel cell of starting is tamed, electricity generation performance improves 12.5%-75%, and sensing sensitivity about promotes 2-3 times.
Detailed description of the invention
Fig. 1 is micro- life of 10 DEG C of acclimated microorganism fuel cells of low temperature and 25 DEG C of room temperature domestications in the embodiment of the present invention 1
Object fuel cell produces electricity voltage by Pb in 25 DEG C of environment2+The result figure of inhibition;
Fig. 2 is micro- life of 10 DEG C of acclimated microorganism fuel cells of low temperature and 25 DEG C of room temperature domestications in the embodiment of the present invention 1
Object fuel cell produces electricity voltage by Pb in 10 DEG C of environment2+The result figure of inhibition;
Fig. 3 is micro- life of 10 DEG C of acclimated microorganism fuel cells of low temperature and 25 DEG C of room temperature domestications in the embodiment of the present invention 1
Comparison of the object fuel cell to the sensing sensitivity of heavy metal lead ion.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
Not constituting a conflict with each other can be combined with each other.
A kind of method promoting microbiological fuel cell electricity production and sensing capabilities provided by the invention, includes the following steps:
In low temperature environment, the anode biomembrane of assembled microbiological fuel cell is tamed, to the microbiological fuel cell
Output voltage stabilization, i.e. the acquisition successful microbiological fuel cell sensor of domestication by low temperature, are obtained specific by domestication by low temperature
The anode electricity production flora of the microbiological fuel cell of structure is fallen, and promotes the electricity generation ability and biography of the microbiological fuel cell sensor
Perceptual energy.
Specifically, this method comprises the following steps:
(1) building of microbiological fuel cell sensor: building microbiological fuel cell sensor, the sensor include string
The anode and cathode of connection;
(2) it the inoculation of microbiological fuel cell sensor: uses containing microbial matrices solution to the anode of the sensor
Biomembrane is inoculated with, and the matrix solution includes sewage;
(3) microbiological fuel cell sensor anode biofilms domestication: the microbiological fuel cell is passed at 10 DEG C or less
The anode biomembrane of sensor is tamed, and replaces matrix solution at regular intervals, defeated to the microbiological fuel cell sensor
Voltage stabilization out, i.e. the acquisition successful microbiological fuel cell sensor of domestication by low temperature.
Low temperature environment actual temp range of the present invention is not higher than 12 DEG C.In some preferred embodiments, the low temperature
Environment actual temp range is 8~12 DEG C.
The present invention carries out domestication by low temperature by the way that the MFC sensor to be placed in low temperature environment, for example the sensor is placed in
In refrigerator, the temperature for controlling matrix solution is in target control temperature.
The microbiological fuel cell sensor (hereinafter referred to as MFC sensor) that the present invention mentions, structure is the same as conventional MFC
Sensor, such as its anode can be graphite plate or other conventional anode materials, and cathode is air cathode, by mixing on stainless (steel) wire
It closes active carbon and carbon black is made or using other common cathode materials.
MFC sensor of the present invention uses inoculation liquid of the sewage as its anode biomembrane, which is that common municipal administration is dirty
Water, for example inoculation domestication is carried out using waste water after urban wastewater treatment firm grid;The matrix solution of the sensor generally further includes
Buffer solution and sodium acetate solution.
In some embodiments, when being inoculated with to the sensor, it is inoculated with sewage in the matrix solution of use and accounts for entire base
50% or so of matter liquor capacity, buffer solution is generally phosphate buffer solution in matrix solution.
In some embodiments, concentration of the sodium acetate in matrix solution is 1g/L.
In the domestication stage, matrix solution of general replacement in every 4-5 days, to the end of taming, after which starts successfully,
The matrix solution of replacement in steady operation period every 1-2 days.
It is also in series with outer connecting resistance in general MFC sensor, the voltage at outer connecting resistance both ends is the defeated of the MFC sensor
Voltage out.
After the sensor successfully starts up, municipal wastewater is not added.To the successful microbiological fuel cell of domestication by low temperature
The heavy metal ion solution of various concentration is added in sensor, by observing sensor output voltage variation, analysis tests it
Sensing capabilities.
The present invention is regulated and controled by temperature, cooperates specific anode biomembrane inoculation liquid, group's knot of optimization anode electricity production bacterium
Structure, improves the electricity generation performance of microbiological fuel cell, while enhancing sensing capabilities.Experimentation discovery domestication by low temperature obtains
Anode biomembrane in relative to room temperature tame, more had fimbriate bacterium pattern, the appearance of pili illustrates its tool
There is the tolerance to low temperature, and in biocoene analysis, the ratio for bacterium of resisting cold is also higher.The advantage of the technology is: micro- life
Object structure of community is affected by temperature larger, can generate response to extraneous temperature change, be easy to change by regulating and controlling temperature
Biology community structure;The electricity production bacterium of domestication by low temperature can resist ambient temperature fluctuation, expand suitable temperature limit, tie up
Efficient electricity generation performance is held, meanwhile, microbiological fuel cell is more conducive in terms of sensing by the structure of community that low temperature optimizes
Using improving sensing capabilities.The technology has reason to application of the promotion microbiological fuel cell in production capacity and sensory field
Value and practice significance.
The following are embodiments:
Embodiment 1
(1) it prepares microbiological fuel cell graphite sheet anode: graphite plate is cut into the small pros of 2cm × 2cm × 0.5cm
Block, successively after 800 mesh, the polishing of 1200 mesh sand paper, in polishing block plus appropriate 0.05 μm of polishing aluminium powder, graphite electrode draws 8 words
Method is processed by shot blasting, after deionized water is cleaned, obtains clean graphite plate electrode.Clean graphite plate is successively immersed third
Ketone, dehydrated alcohol, deionized water, are respectively cleaned by ultrasonic 15min, and 70 DEG C of baking ovens dry 4h, microbiological fuel cell stone can be obtained
Black plate anode.
(2) it prepares microbial fuel cell air cathode: 50 mesh stainless (steel) wires being cut into the circle of diameter 3.8cm, successively
Acetone, dehydrated alcohol, deionized water are immersed, 15min is respectively cleaned by ultrasonic, 70 DEG C of baking oven drying 4h are spare.Weigh Kynoar
(PVDF) powder 5g is dissolved in 50mL n,N-dimethylacetamide in 150mL small beaker, and rotor is added, and rim of a cup sealed membrane is close
Envelope is placed on magnetic stirring apparatus 80 DEG C, 700r/min, stirs 8h, obtains PVDF solution.Weigh 300mg active carbon powder, 30mg
Acetylene black powder mixes, and 1mL PVDF solution is added, and round steel mesh side is coated uniformly on after stirring, and deionized water is impregnated
Microbial fuel cell air cathode can be obtained after taking out natural air drying 12h after 15min.
(3) Low temperature regulation microbiological fuel cell tames process: assembling microbiological fuel cell utilizes titanium silk and external
1000 Ohmic resistances connect microbiological fuel cell anode and cathode, connect electrical circuit, microbiological fuel cell is placed in 10 DEG C of environment
In tamed, until its complete stability run, using waste water after municipal sewage plant's grid as produce electricity bacterium inoculation liquid, take one
Half 50mM phosphate buffer and half inoculation liquid are mixed, and anhydrous sodium acetate is added, its concentration is made to reach 1g/L.Domestication
After the completion, it does not need to add inoculation liquid again in matrix.The matrix of replacement in domestication 5 days, the base of replacement in steady operation period 2 days
Matter.After microbiological fuel cell stable operation, lead standard solution is injected into chamber, its concentration is made to reach 1mg/L, is observed micro-
The variation of biological fuel cell output voltage.
Comparative example 1
For other conditions with embodiment 1, difference is that its step (3) acclimation temperature is 25 DEG C of room temperature.
Embodiment 2
(1) it prepares microbiological fuel cell graphite sheet anode: graphite plate is cut into the small pros of 2cm × 2cm × 0.5cm
Block, successively after 800 mesh, the polishing of 1200 mesh sand paper, in polishing block plus appropriate 0.05 μm of polishing aluminium powder, graphite electrode draws 8 words
Method is processed by shot blasting, after deionized water is cleaned, obtains clean graphite plate electrode.
Clean graphite plate is successively immersed into acetone, dehydrated alcohol, deionized water, is respectively cleaned by ultrasonic 15min, 70 DEG C of baking ovens
4h is dried, microbiological fuel cell graphite sheet anode can be obtained.
(2) it prepares microbial fuel cell air cathode: 50 mesh stainless (steel) wires being cut into the circle of diameter 3.8cm, successively
Acetone, dehydrated alcohol, deionized water are immersed, 15min is respectively cleaned by ultrasonic, 70 DEG C of baking oven drying 4h are spare.Weigh Kynoar
(PVDF) powder 5g is dissolved in 50mL n,N-dimethylacetamide in 150mL small beaker, and rotor is added, and rim of a cup sealed membrane is close
Envelope is placed on magnetic stirring apparatus 80 DEG C, 700r/min, stirs 8h, obtains PVDF solution.Weigh 300mg active carbon powder, 30mg
Acetylene black powder mixes, and 1mL PVDF solution is added, and round steel mesh side is coated uniformly on after stirring, and deionized water is impregnated
Microbial fuel cell air cathode can be obtained after taking out natural air drying 12h after 15min.
(3) microbiological fuel cell tames process: assembling microbiological fuel cell utilizes titanium silk and external 1000 ohm of electricity
Resistance connection microbiological fuel cell anode and cathode, connects electrical circuit, microbiological fuel cell is placed in 10 DEG C of environment and is tamed,
It is run to its complete stability, using waste water after municipal sewage plant's grid as electricity production bacterium inoculation liquid, takes half 50mM phosphoric acid
Salt buffer and half inoculation liquid are mixed, and anhydrous sodium acetate is added, its concentration is made to reach 1g/L.After the completion of domestication, base
It does not need to add inoculation liquid again in matter.The matrix of replacement in domestication 5 days, the matrix of replacement in steady operation period 2 days.To micro- life
After the operation of object stable fuel cell, lead standard solution is injected into chamber, its concentration is made to reach 2mg/L, observes Microbial fuel
Cell output voltage variation.
Comparative example 2
For other conditions with embodiment 2, difference is that its step (3) acclimation temperature is 25 DEG C of room temperature.
Embodiment 3
(1) it prepares microbiological fuel cell graphite sheet anode: graphite plate is cut into the small pros of 2cm × 2cm × 0.5cm
Block, successively after 800 mesh, the polishing of 1200 mesh sand paper, in polishing block plus appropriate 0.05 μm of polishing aluminium powder, graphite electrode draws 8 words
Method is processed by shot blasting, after deionized water is cleaned, obtains clean graphite plate electrode.
Clean graphite plate is successively immersed into acetone, dehydrated alcohol, deionized water, is respectively cleaned by ultrasonic 15min, 70 DEG C of baking ovens
4h is dried, microbiological fuel cell graphite sheet anode can be obtained.
(2) it prepares microbial fuel cell air cathode: 50 mesh stainless (steel) wires being cut into the circle of diameter 3.8cm, successively
Acetone, dehydrated alcohol, deionized water are immersed, 15min is respectively cleaned by ultrasonic, 70 DEG C of baking oven drying 4h are spare.Weigh Kynoar
(PVDF) powder 5g is dissolved in 50mL n,N-dimethylacetamide in 150mL small beaker, and rotor is added, and rim of a cup sealed membrane is close
Envelope is placed on magnetic stirring apparatus 80 DEG C, 700r/min, stirs 8h, obtains PVDF solution.Weigh 300mg active carbon powder, 30mg
Acetylene black powder mixes, and 1mL PVDF solution is added, and round steel mesh side is coated uniformly on after stirring, and deionized water is impregnated
Microbial fuel cell air cathode can be obtained after taking out natural air drying 12h after 15min.
(3) microbiological fuel cell tames process: assembling microbiological fuel cell utilizes titanium silk and external 1000 ohm of electricity
Resistance connection microbiological fuel cell anode and cathode, connects electrical circuit, microbiological fuel cell is placed in 10 DEG C of environment and is tamed,
It is run to its complete stability, using waste water after municipal sewage plant's grid as electricity production bacterium inoculation liquid, takes half 50mM phosphoric acid
Salt buffer and half inoculation liquid are mixed, and anhydrous sodium acetate is added, its concentration is made to reach 1g/L.After the completion of domestication, base
It does not need to add inoculation liquid again in matter.The matrix of replacement in domestication 5 days, the matrix of replacement in steady operation period 2 days.To micro- life
After the operation of object stable fuel cell, lead standard solution is injected into chamber, its concentration is made to reach 3mg/L, observes Microbial fuel
Cell output voltage variation.
Comparative example 3
For other conditions with embodiment 3, difference is that its step (3) acclimation temperature is 25 DEG C of room temperature.
Analysis of experimental results:
Fig. 1 is micro- life of 10 DEG C of acclimated microorganism fuel cells of low temperature and 25 DEG C of room temperature domestications in the embodiment of the present invention 1
Object fuel cell produces electricity voltage by Pb in 25 DEG C of environment2+The result figure of inhibition;Fig. 2 is 10 DEG C of low temperature in the embodiment of the present invention 1
Acclimated microorganism fuel cell and 25 DEG C of acclimated microorganism fuel cells of room temperature produce electricity voltage by Pb in 10 DEG C of environment2+Suppression
The result figure of system;Fig. 3 be in the embodiment of the present invention 1 10 DEG C of acclimated microorganism fuel cells of low temperature with 25 DEG C of room temperature tame it is micro-
Comparison of the biological fuel cell to the sensing sensitivity of heavy metal lead ion.
It can be seen that the microbiological fuel cell by 10 DEG C of domestications by low temperature compared to 25 DEG C of room temperature from Fig. 1, Fig. 2 and Fig. 3
Acclimated microorganism fuel cell has the sensing sensitivity of heavy metal lead ion and is greatly promoted, voltage change it is exhausted
It is bigger to being worth, it can significantly find out the voltage dip in the short time, this is more conducive to the front end early warning of sewage, sensing sensitivity
2-3 times is about promoted compared with room temperature domestication.And the microbiological fuel cell after 10 DEG C of domestications by low temperature has the fluctuation of ambient temperature
There is better anti-interference, electricity generation performance is still maintained at a higher level, and electricity production remains to reach at low ambient temperatures
320mV, and the fuel cell without domestication by low temperature, at low ambient temperatures, electricity generation performance is decreased obviously, and can only achieve 180mV
Electricity generation performance improves 77%.Illustrate that the microbiological fuel cell after 10 DEG C of domestications by low temperature, anode produce electricity bacterium flora knot
Structure is changed, and is more conducive to promoting microbiological fuel cell electricity production and sensing capabilities.
In addition, can also be seen that detection electricity production voltage is by Pb either in 25 DEG C of environment from Fig. 1, Fig. 2 and Fig. 32+Inhibit
Result or 10 DEG C of environment in detection electricity production voltage by Pb2+It is inhibiting as a result, it can be seen that, 10 DEG C of low temperature tame and obtain
Sensor its produce electricity voltage and to Pb2+Sensing response voltage change absolute value all than 25 DEG C of domestications of room temperature when it is high, illustrate low temperature tame and docile
Change the work for not only contributing to low temperature environment lower sensor, and also help the application under normal temperature environment, illustrates domestication by low temperature
The operating temperature range of the sensor can be widened.
Experiment confirms to tame above-mentioned MFC sensor at 8-12 DEG C of low temperature range to can be improved its electrochemical sensing
Performance, and its use temperature range can be widened.
The present invention tames environment by Low temperature regulation microbiological fuel cell, and optimization electricity production bacterium structure of community improves micro- life
The electricity production of object fuel cell and sensing capabilities, belong to bioelectrochemistry and bio-sensing field.The present invention passes through the micro- life of cold-starting
Object fuel cell, optimization electricity production bacterium structure of community, not only increases the electricity generation performance of microbiological fuel cell, has simultaneously amplified electricity
Chemical signal enhances sensing capabilities.This method is simple, environmental-friendly, optimizes electricity production bacterium structure, not only increases microorganism
Fuel cell waste water energy recovery efficiency, while microbiological fuel cell sensing capabilities are improved, it is a kind of collection sewage treatment energy
Source recycling and monitoring water quality on line are in the environmentally protective water process and water quality monitoring technology of one.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should all include
Within protection scope of the present invention.
Claims (7)
1. a kind of method for promoting microbiological fuel cell electricity production and sensing capabilities, which is characterized in that in low temperature environment, to micro-
The anode biomembrane of biological fuel cell is tamed, and to the microbiological fuel cell output voltage stabilization, i.e. acquisition low temperature is tamed and dociled
Change successful microbiological fuel cell sensor, the anode of the microbiological fuel cell of specific structure is obtained by domestication by low temperature
Electricity production flora is fallen, and promotes the electricity generation ability and sensing capabilities of the microbiological fuel cell sensor;The wherein low temperature environment tool
Temperature range is not higher than 12 DEG C.
2. the method as described in claim 1, which comprises the steps of:
(1) building of microbiological fuel cell sensor: building microbiological fuel cell sensor, which includes concatenated
Anode and cathode;
(2) inoculation of microbiological fuel cell sensor: using the matrix solution containing microorganism to the Anode of the sensor
Object film is inoculated with, and the matrix solution includes sewage;
(3) microbiological fuel cell sensor anode biofilms domestication: the microbiological fuel cell is sensed at low ambient temperatures
The anode biomembrane of device is tamed, and certain interval of time replaces matrix solution, is exported to the microbiological fuel cell sensor
Voltage stabilization, i.e. the acquisition successful microbiological fuel cell sensor of domestication by low temperature.
3. method according to claim 1 or 2, which is characterized in that the low temperature environment actual temp range is 8-12 DEG C.
4. method according to claim 2, which is characterized in that the anode is graphite plate, and the cathode is air cathode,
Cathode material is made of mixed active charcoal and carbon black on stainless (steel) wire.
5. method according to claim 2, which is characterized in that the sewage is municipal wastewater.
6. method according to claim 2, which is characterized in that the matrix solution further includes that buffer solution and sodium acetate are molten
Liquid.
7. method according to claim 2, which is characterized in that further comprise the steps of:
(4) microbiological fuel cell heavy metal toxicity sensing testing: to the successful microbiological fuel cell sensor of domestication by low temperature
The middle heavy metal ion solution that various concentration is added, by observing sensor output voltage variation, its sensitivities is tested in analysis
Energy.
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CN115340969A (en) * | 2022-09-20 | 2022-11-15 | 中国科学院重庆绿色智能技术研究院 | Flora domestication method and application of BOD (biochemical oxygen demand) microbial electrochemical sensor |
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