CN104984994B - Device and method for removing polychlorinated biphenyl in bottom mud through microorganism electrolytic tank - Google Patents

Device and method for removing polychlorinated biphenyl in bottom mud through microorganism electrolytic tank Download PDF

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CN104984994B
CN104984994B CN201510401590.3A CN201510401590A CN104984994B CN 104984994 B CN104984994 B CN 104984994B CN 201510401590 A CN201510401590 A CN 201510401590A CN 104984994 B CN104984994 B CN 104984994B
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anode
chamber
electrode
bed mud
polychlorinated biphenyls
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CN104984994A (en
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冯春华
余辉
刘小平
杨晓双
李晨晨
韦朝海
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South China University of Technology SCUT
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Abstract

The invention belongs to the technical field of soil restoration and discloses a device and method for removing polychlorinated biphenyl in bottom mud through a microorganism electrolytic tank. The microorganism electrolytic tank is established, hybrid electrochemical activity bacteria and perchlorate domesticated bacteria are inoculated to an anode chamber, soil polluted by polychlorinated biphenyl is added, a nutrient solution is supplemented to the rest part, the water phase-bottom mud anode chamber is formed, anode potential is controlled to be kept constant through an electrochemical work station, growth and propagation of the hybrid electrochemical activity bacteria and the perchlorate domesticated bacteria can be promoted, dechloridation efficiency can be greatly improved, and constant bioelectric currents can be generated. By the adoption of the device and method, natural restoration and toxicity reduction can be conducted on the polluted soil, and good environmental protection benefits are achieved.

Description

The device and method of Polychlorinated biphenyls in bed mud is removed using microorganism electrolysis cell
Technical field
The invention belongs to technical field of soil remediation, and in particular to one kind removes many chlorine in bed mud using microorganism electrolysis cell The device and method of biphenyl.
Background technology
Polychlorinated biphenyls are due to the excellent physicochemical property such as its thermodynamic stability, chemical inertness, low flammability, high conductivity And be widely applied in field of industrial production, middle 1960s, whole world Polychlorinated biphenyls annual production is about 100,000 tons, From 20 century 70s, close ten thousand tons of the Polychlorinated biphenyls annual production of China.As a kind of typical persistence organic pollutant, The genotoxic potential and structural stability of Polychlorinated biphenyls brings serious harm to health and natural environment, therefore seeks one kind The method for effectively repairing polluted soil is extremely urgent.Currently for Polychlorinated biphenyls minimizing technology include traditional biodegrading process and Emerging degradation technique, traditional biodegrading process seals landfill method, high temperature incineration method up for safekeeping;Emerging degradation technique has supercritical water oxidation Method, dissolving electronic technology, chemical reduction method, photochemical degradating method and microbial degradation method.The selection of Polychlorinated biphenyls minimizing technology Depend primarily on its load configuration, species and concentration value, wherein microbial degradation method compared with other a few class methods for, have into This cheap, easy to operate, non-secondary pollution, possesses the advantages such as in-situ immobilization meaning, while degradation rate is slow, needs nondominant hand Duan Tigao Polychlorinated biphenyls biodegradabilities are also that the shortcoming of microbial degradation method is located.
In recent years, microbiological fuel cell technology is in wastewater treatment, desalination, prepares application in biosensing apparatus increasingly Maturation, derivative bed mud-microbiological fuel cell technology promotes the natural reparation of complicated Organic substance such as phenol, phenanthrene, oil Gradually paid attention to.
Therefore running cost is being saved, it is to avoid on the premise of secondary pollution, we are come using the mode of microorganism electrolysis cell Reinforcing contaminated soil in Polychlorinated biphenyls reduction conversion and oxidation removal, for Polychlorinated biphenyls based technique for in-situ remediation provide it is new Thinking.
The content of the invention
In order to solve the shortcoming and defect part of prior art, the primary and foremost purpose of the present invention is to provide a kind of using micro- life Thing electrolyzer removes the device of Polychlorinated biphenyls in bed mud.
Another object of the present invention is to provide a kind of method that utilization said apparatus remove Polychlorinated biphenyls in bed mud.
The object of the invention is achieved through the following technical solutions:
A kind of utilization microorganism electrolysis cell removes the device of Polychlorinated biphenyls in bed mud, by double-chamber microbiological electrolyzer and Duo Tong Road electrochemical workstation composition, double-chamber microbiological electrolyzer includes anode chamber and cathode chamber, anode chamber and cathode chamber by sun from Proton exchange is separated, and anode chamber arranges anode electrode and reference electrode, and cathode chamber arranges cathode electrode, anode electrode, negative electrode Electrode connects multi-channel electrochemical work station with reference electrode, and the current potential of the controllable anode chamber of work station keeps constant (relative ginseng Than electrode).
Described reference electrode is saturated calomel electrode, described anode electrode and the preferred carbon paper of the material of cathode electrode, Graphite felt, carbon cloth;More preferably through the carbon paper of pretreatment, described preprocess method rinses carbon paper for deionized water anode After drying, it is placed in ethanol under room temperature and soaks 24h, subsequently the heat treatment 12h at 100 DEG C;Carbon paper after heat treatment is placed on alkali 24h is soaked in liquid and the mixed liquor of ethanol, last deionized water is rinsed and dried;Negative electrode is more preferably through the graphite of pretreatment Felt, described preprocess method is that graphite felt is immersed in the hydrogen peroxide that mass fraction is 10%, decocting in water 2h at 90 DEG C, go from The decocting in water 2h after sub- water is rinsed and in deionized water, takes out drying.
Multi-channel electrochemical work station used is multichannel potentiostat.
A kind of method that utilization said apparatus remove Polychlorinated biphenyls in bed mud, including following operating procedure:
The hybrid electrochemical active bacteria through domestication is inoculated with to anode chamber, adds sodium acetate as electron donor, be with pH 7.0 phosphate buffered solution fills it up with anode chamber's remainder and cathode chamber with the mixed liquor of culture fluid, observes mixing electrification Learning active bacteria can persistently produce electricity, i.e., stably after biofilm, outwell the liquid in anode chamber, and inoculation perchlorate domestication bacterium adds Bed mud containing Polychlorinated biphenyls;Anode chamber's residual volume and cathode chamber fill perchlorate domestication basic culture solution, and basis Culture fluid Jing exposes nitrogen treatment and removes dissolved oxygen therein;The three of double-chamber microbiological electrolyzer are connected using multichannel potentiostat Electrode, sets anode potential as+0.2V (relative to reference electrode), regular replenishment sodium acetate after commencement of commercial operation, completes to bed mud The removal of middle Polychlorinated biphenyls.
Concentration of the sodium acetate in anode chamber is preferably 10mM.
Described hybrid electrochemical active bacteria is aerobic activated sludge in double-chamber microbiological electrolyzer, is applying current potential thorn Swash and keep concentration in anode room for 10mM sodium acetate as electron donor in the case of tame and obtain;The perchlorate is tamed and dociled Change bacterium is anaerobic activated sludge, using sulphur powder as electron donor, in the case of improving the concentration of substrate perchlorate, carry out by Obtain after level domestication.
The pH is that 7.0 phosphate buffer includes 22.2g/L Na with the composition of the mixed liquor of nutritional solution2HPO4· 12H2O、5.92g/L NaH2PO4·2H2O、5.88g/L NaCl、1.0g/L NaHCO3、0.10g/L FeSO4、0.10g/L KCl、0.015g/L CaCl2、0.25g/L NH4Cl, 10mL/L mineral solution and 10mL/L vitamin solution.
The composition of the perchlorate domestication basic culture solution includes 0.41g/L NH4HCO3, 0.25g/L K2HPO4, 2.70g/L NaHCO3, 0.005g/L Ca (OH)2, 10mL/L vitamins solution and 10mL/L mineral solutions.
The mineral solution composition includes 1.5g/L C6H6NO6·3Na·12H2O、0.13g/L ZnCl2、3.0g/L MgSO4、0.01g/L CuSO4·5H2O、0.5g/L MnSO4·H2O、0.01g/L AlK(SO4)2·12H2O、1.0g/L NaCl、0.01g/L H3BO3、0.1g/L FeSO4·7H2O、0.025g/L Na2MoO4、0.1g/L CaCl2·2H2O、 0.024g/L NiCl2·6H2O、0.1g/L CoCl2·6H2O and 0.025g/L Na2WO4·2H2O。
Described vitamin solution composition include 2.0mg/L biotin, 2.0mg/L Folic Acid, 10.0mg/L vitamin Bies, 5.0mg/L thiamines, 5.0mg/L riboflavin, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenates, 0.1mg/L Vitamin B12,5.0mg/ The acid of L para-aminotoluenes and 5.0mg/L thioctic acid.
The present invention principle be:
The microorganism electrolysis cell with anode chamber and cathode chamber is built, anode chamber is water phase-bed mud (including electrode) system. Potential value using electrochemical operation stand control anode chamber is constant in+0.2V, can promote carbon paper electrode surface electrochemistry active bacteria Growth and produce bioelectric current, supplementary sodium acetate serves as electron donor can maintain specificity dehalogenation bacterium to take off Polychlorinated biphenyls Halogen is breathed, and there is mutual synergism between simultaneous electrochemical active bacteria and specificity dehalogenation bacterium, can further promote electronics to turn It is moved into and realizes the reduction dechlorination process of Polychlorinated biphenyls, and two class Pseudomonas can utilize the organic matter enriched in load bed mud to meet certainly Body grows.
, preferably through the carbon paper of pretreatment, carbon paper is with higher specific surface area, good biology for the material of anode electrode The compatibility and reasonable prices, and will not absorption carriage Polychlorinated biphenyls soil particle, carbon paper is carried out into pretreatment and is conducive to carrying High its stability, electric conductivity and biocompatibility.
Had the advantage that by the method for the present invention and device and beneficial effect:
(1) present invention using microorganism electrolysis cell to realize bed mud in Polychlorinated biphenyls reduction remove, on the one hand can be big Amplitude realizes pollution amelioration, toxicity reduction, on the other hand can avoid the problem of the secondary pollution that conventional art brings and emerging It is with high costs that technology is brought, the big problem of operation difficulty.
(2) under the hybrid electrochemical active bacteria and perchlorate that the present invention is adopted tames bacterium for specified conditions, specific battalion is used Nutrient solution carries out domestication culture, with efficient electricity generation ability and efficient dehalogenation ability;
(3) device and method of the invention can be also used for other halos except applying the reduction in Polychlorinated biphenyls to remove For example on the pollution amelioration of chlorobenzene, PBDE etc. of Organic substance;
(4) present invention bed mud used is retrieved from exemplary electronic rubbish and disassembles field stream nearby, after testing wherein containing a large amount of High concentration cl generation complicated Organic substance, including Polychlorinated biphenyls, therefore the method for the present invention can be applied in actual Polluted Soil with device In the in-situ immobilization of earth.
Description of the drawings
Fig. 1 is a kind of utilization microbiological fuel cell of embodiment 1 while the apparatus structure of degradation of phenol and ammonia nitrogen is illustrated Figure, labelling is described as follows in figure:1- anode chambers, 2- cathode chambers, 3- cation exchange membranes, 4- electrochemical workstations, 5- anodes add Liquid mouth, 6- anode electrodes, 7- reference electrodes, 8- negative electrode filling openings, 9- cathode electrodes, 10- external circuit;
Fig. 2 is Polychlorinated biphenyls (2,3,4,5- tetrachloro biphenyls, PCB61) concentration in embodiment 1~2 and comparative example 1~3 Versus time curve;
Fig. 3 is Polychlorinated biphenyls intermediates 2,3,5- trichloro biphenyls in embodiment 1~2 and comparative example 1~3 And 2,4,5- trichloro biphenyls (PCB29) concentration versus time curve (PCB23);
Fig. 4 is embodiment 1 and current intensity versus time curve in embodiment 2.
Specific embodiment
With reference to embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited In this.
Hybrid electrochemical active bacteria and perchlorate domestication bacterium used in following examples is screened by the following method And domestication is obtained:
The activated sludge 10mL of Shaoguan City of Guangdong Province Shaoguan Iron & Steel Plant coking chemical waste water second phase process engineering O1 section is taken from, is added To the anode of double-chamber microbiological electrolyzer, the wherein capacity of battery is 200mL, anode chamber and each 100mL of cathode chamber;In anode chamber Add 1mL, concentration for 1M sodium acetate as electron donor, anode chamber's remainder and cathode chamber fill it up with the phosphoric acid of pH=7.0 Buffer solution and the mixed liquor of culture fluid, install standard saturated calomel reference electrode, form three-electrode system;Assemble above-mentioned Battery be put in 30 DEG C of constant temperature and humidity incubators, galvanic anode, negative electrode, reference electrode connect respectively the work of electrochemical workstation Make electrode wires, to electrode wires and reference electrode wire, and impose the current potential of 0.2V vs SCE, changed fresh medium simultaneously every five days Supplement same volume, with the sodium acetate of concentration;After one month, add after sodium acetate in microbiological fuel cell battery, can quickly ring Answer and bioelectric current can reach 10mA-20mA, tame successfully, obtain hybrid electrochemical active bacteria.
The anaerobism section activated sludge about 200ml that Guangzhou, Guangdong drips Kau municipal sewage plant is taken from, being placed in volume is In the blue lid bottle of 1L, remainder supplements basic culture solution and adds the perchlorate of 10g distillation sulphur powders and 10mg/L as electricity Sub- donor and electron acceptor, expose nitrogen drive away be placed in after oxygen be set as 150 revs/min, in 30 DEG C of shaking table;Regular (5-10 My god) stand and change culture supernatants and the concentration of perchlorate is further added by gradient according to perchlorate reduction's effect, Trimestral to adapt to after culture, the perchlorate of 100mg/L can be reduced into chloride ion in two days, tame successfully, obtain perchloric acid Salt tames bacterium.
Load bed mud used in following examples takes from Guangdong Province Qingyuan City Long Tang towns electronic waste and disassembles factory's (north latitude 23 ° of 36'12.30 ", the bed mud of neighbouring below the river cement interface 10cm of 113 ° of 04'38.84 ") of east longitude, the sludge Jing sieves for taking The wherein impurity such as branch, leaveves, stone is removed in blanking, is preserved under room temperature.
Embodiment 1
As shown in figure 1, a kind of utilization microorganism electrolysis cell of the present embodiment removes the device of Polychlorinated biphenyls in bed mud, by double Chamber microbiological electrolyzer and electrochemical workstation are constituted, and double-chamber microbiological electrolyzer includes anode chamber 1 and cathode chamber 2, anode chamber Separated by cation exchange membrane 3 with cathode chamber, anode chamber arranges galvanic anode filling opening 5, anode electrode 6 and reference electrode 7, cathode chamber arranges cell cathode filling opening 8 and cathode electrode 9, and anode electrode, reference electrode and cathode electrode pass through external circuit 10 are connected with electrochemical workstation 4, and electrochemical workstation constant control anode keeps the current potential of+0.2V.
The device of the present embodiment is used for the method for removing Polychlorinated biphenyls in bed mud, concretely comprises the following steps:To anode chamber's inoculation 10ml adds the sodium acetate that 1ml concentration is 1M as electron donor through the hybrid electrochemical activity bacterium solution of domestication, is with pH 7.0 phosphate buffered solution fills it up with anode chamber's remainder and cathode chamber with the mixed liquor of culture fluid, observes electricity production bacterium energy Persistently produce electricity, i.e., stably after biofilm, outwell the liquid in anode chamber, add the perchlorate domestication bacterium solution of 30ml, and to take from The bed mud of Qingyuan City is 100uM/kg as load bed mud, the concentration for controlling Polychlorinated biphenyls (2,3,4,5- tetrachloro biphenyls, PCB61), Anode chamber's remainder and cathode chamber fill perchlorate domestication basic culture solution;The carbon paper working electrode of anode chamber, saturation To electrode, respectively (Shanghai Chen Hua Instrument Ltd. produces the graphite carbon felt of calomel reference electrode and cathode chamber with electrochemical workstation The passage potentiostat of CHI1000C types eight) corresponding joints be connected, and apply the potential value of+0.2V vs SCE;After self-starting, The supplementary 1ml every 20 days, concentration is the sodium acetate of 1M.
The carbon paper working electrode of the present embodiment using front through following methods pretreatment:Deionized water rinses carbon paper After drying, it is placed in ethanol under room temperature and soaks 24h, subsequently the heat treatment 12h at 100 DEG C;Carbon paper after heat treatment is placed on alkali liquor (0.3M) 24h is soaked with the mixed liquor of ethanol, last deionized water is rinsed and dried;Graphite carbon felt is to electrode using front Through following methods pretreatment:Graphite felt is immersed in the hydrogen peroxide that mass fraction is 10%, decocting in water 2h, deionization at 90 DEG C Water rinse after and in deionized water decocting in water 2h, take out drying.
Described pH is that 7.0 phosphate buffered solution includes 22.2g/L Na with the mixed liquor composition of culture fluid2HPO4、 5.92g/L NaH2PO4、1.0g/L NaHCO3、0.10g/L FeSO4、0.10g/L KCl、0.015g/L CaCl2、0.25g/ LNH4Cl, 10mL/L mineral solution and 10mL/L vitamins;Perchlorate domestication basic culture solution composition includes 0.41g/L NH4HCO3, 0.25g/L K2HPO4, 2.70g/L NaHCO3, 0.005g/L Ca (OH)2, 10mL/L vitamins solution and 10mL/L Mineral solution;Vitamin solution composition is:2.0mg/L biotin, 2.0mg/L Folic Acid, 10.0mg/L vitamin Bies, 5.0mg/ L thiamines, 5.0mg/L riboflavin, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenates, 0.1mg/L Vitamin B12,5.0mg/L is to ammonia Base toluic acid, 5.0mg/L thioctic acid;Mineral solution composition is:1.5g/L C6H6NO6·3Na·12H2O, 0.13g/L ZnCl2, 3.0g/L MgSO4,0.01g/L CuSO4·5H2O, 0.5g/L MnSO4·H2O, 0.01g/L AlK (SO4)2· 12H2O, 1.0g/L NaCl, 0.01g/L H3BO3, 0.1g/L FeSO4·7H2O, 0.025g/L Na2MoO4, 0.1g/L CaCl2·2H2O, 0.024g/L NiCl2·6H2O, 0.1g/L CoCl2·6H2O and 0.025g/L Na2WO4·2H2O。
Embodiment 2
The apparatus and method that a kind of utilization microorganism electrolysis cell of the present embodiment removes Polychlorinated biphenyls in bed mud, with embodiment 1 compares, and difference is the sodium acetate without regular replenishment 1ml concentration for 1M, and remainder is identical.
Comparative example 1
The apparatus and method that a kind of utilization microorganism electrolysis cell of this comparative example removes Polychlorinated biphenyls in bed mud, with reality Apply example 1 to compare, difference is that the electrode of the anode chamber and the cathode chamber is not connected with electrochemical workstation, and remainder is identical.
Comparative example 2
The apparatus and method that a kind of utilization microorganism electrolysis cell of this comparative example removes Polychlorinated biphenyls in bed mud, with reality Apply example 2 to compare, difference is that the electrode of the anode chamber and the cathode chamber is not connected with electrochemical workstation, and remainder is identical.
Comparative example 3
The apparatus and method that a kind of utilization microorganism electrolysis cell of this comparative example removes Polychlorinated biphenyls, with embodiment 2 Compare, difference is not to be inoculated with hybrid electrochemical active bacteria and perchlorate domestication bacterium, and load bed mud is through sterilization treatment And the electrode of the anode chamber and the cathode chamber is not connected with electrochemical workstation, remaining operation is identical.
Device and method in above-described embodiment and comparative example is used for the effect that Polychlorinated biphenyls are removed in bed mud and compares:
Tetrachloro biphenyl PCB61 concentration versus time curve such as Fig. 2 institutes in embodiment 1~2 and comparative example 1~3 Show;Polychlorinated biphenyls intermediates trichloro biphenyl PCB23 and PCB29 concentration versus time curve is as shown in Figure 3.By scheming 2 and Fig. 3 can be seen that:The concentration of target Polychlorinated biphenyls PCB61 is not observed in (comparative example 3) reactor that sterilization treatment is crossed To reduce and catabolite does not observe generation yet;And it is inoculated with the reaction of hybrid electrochemical active bacteria and perchlorate domestication bacterium solution In device, wherein in the case of not connected electrochemical workstation and supplementary extra electron donor sodium acetate (comparative example 2), just Beginning concentration is 100 μm of ol kg-1PCB61 in 22.6 μm of ol kg-1Generation reduction conversion, is not connected with electrochemical workstation but benefit Filling extra electron donor sodium acetate then has 35.8 μm of ol kg-1Generation reduction conversion (comparative example 1);On this basis, pass through Electrochemical workstation applies in the reactor stimulated with+0.2V constant potentials, wherein connecting electrochemical workstation but not supplementing extra In the case of electron donor sodium acetate (embodiment 2), initial concentration is 100 μm of ol kg-1PCB61 in 57.7 μm of ol kg-1Send out Survive former conversion, and while connecting electrochemical workstation and supplement extra electron donor sodium acetate (embodiment 1) then has 78.2 μ mol kg-1Generation reduction conversion.This explanation applies the current potential and regular replenishment electronics of+0.2V by electrochemical workstation in anode Donor sodium acetate has to maintain its concentration to hybrid electrochemical active bacteria and perchlorate domestication bacterium decomposition and inversion Polychlorinated biphenyls Greatly effect.In addition from Fig. 3 it is also seen that:The yield of PCB23 is apparently higher than PCB29, and Polychlorinated biphenyls PCB61's is de- Chlorine occurs mainly in para-position and meta, and corresponding dechlorination product is respectively PCB23 and PCB29, therefore the hybrid electrochemical of the present invention Active bacteria and perchlorate tame bacterium based on the para-position dechlorination to Polychlorinated biphenyls.
Embodiment 1 is with current intensity versus time curve in embodiment 2 respectively as (a) and (b) in Fig. 4 is shown.By (a) and (b) can be seen that in Fig. 4:Work station monitors the change of electric current density Development pattern therein, occurs in reaction In 100 days, 0.29mA cm of the electric current density from initial a cycle in embodiment 1-2It is down to the 0.19mA in the tenth cycle cm-2Then continue to be reduced to 0.12mA cm-2;And the electric current density in embodiment 2 is from the 0.031mA of initial a cycle cm-2It is down to the 0.022mA cm in the tenth cycle-2Then continue to be reduced to 0.010mA cm-2
Learnt by result above, it is many in the case of abundant hybrid electrochemical active bacteria and perchlorate domestication bacterium Chlordiphenyl can occur a small amount of reduction dechlorination process, and can meet dehalogenation breathing to electricity using the already present organic matter of load bed mud The demand of sub- donor, and pass through electrochemical workstation and apply constant current potential stimulation to reaction system, mixing electricity can not only be strengthened The growth of chemism bacterium simultaneously produces bioelectric current, while can also promote the growth of related dehalogenation bacterium (perchlorate tames bacterium) simultaneously Reinforcing dehalogenation process.
Above-described embodiment is the present invention preferably embodiment, but embodiments of the present invention not by above-described embodiment Limit, other any spirit and the changes, modification, replacement made under principle without departing from the present invention, combine, simplification, Equivalent substitute mode is should be, is included within protection scope of the present invention.

Claims (7)

1. a kind of method that utilization microorganism electrolysis cell device removes Polychlorinated biphenyls in bed mud, it is characterised in that:Described device by Double-chamber microbiological electrolyzer and multichannel potentiostat are constituted, and the double-chamber microbiological electrolyzer includes anode chamber and cathode chamber, Anode chamber and cathode chamber are separated by cation exchange membrane, and anode chamber arranges anode electrode and reference electrode, and cathode chamber is arranged Cathode electrode;Anode electrode, cathode electrode connect multichannel potentiostat, multichannel potentiostat control anode with reference electrode The current potential of room keeps constant;Methods described includes following operating procedure:
The hybrid electrochemical active bacteria through domestication is inoculated with to anode chamber, adds sodium acetate as electron donor, be 7.0 with pH Phosphate buffered solution fills it up with anode chamber's remainder and cathode chamber with the mixed liquor of culture fluid, observes hybrid electrochemical activity Bacterium can persistently produce electricity, i.e., stably after biofilm, outwell the liquid in anode chamber, and inoculation perchlorate domestication bacterium is added containing many The bed mud of chlordiphenyl;Anode chamber's residual volume and cathode chamber fill perchlorate domestication basic culture solution, and basic culture solution Jing exposes nitrogen treatment and removes dissolved oxygen therein;Three electricity of double-chamber microbiological electrolyzer are connected using multichannel potentiostat Pole, sets anode potential as+0.2V, and regular replenishment sodium acetate after commencement of commercial operation completes the removal to Polychlorinated biphenyls in bed mud.
2. the method that a kind of utilization microorganism electrolysis cell device according to claim 1 removes Polychlorinated biphenyls in bed mud, its It is characterised by:Described reference electrode is saturated calomel electrode, and described anode electrode and the material of cathode electrode is carbon paper, stone Black felt or carbon cloth.
3. the method that a kind of utilization microorganism electrolysis cell device according to claim 2 removes Polychlorinated biphenyls in bed mud, its It is characterised by:The material of the anode electrode is the carbon paper through pretreatment, and described preprocess method will for deionized water Carbon paper is rinsed after drying, is placed in ethanol under room temperature and is soaked 24h, subsequently the heat treatment 12h at 100 DEG C, carbon after heat treatment Paper is placed in the mixed liquor of alkali liquor and ethanol and soaks 24h, and last deionized water is rinsed and dried;The material of the cathode electrode It is the graphite felt through pretreatment, described preprocess method is that graphite felt is immersed in into the hydrogen peroxide that mass fraction is 10% In, decocting in water 2h at 90 DEG C, the decocting in water 2h after deionized water rinsing and in deionized water take out drying.
4. the method that a kind of utilization microorganism electrolysis cell device according to claim 1 removes Polychlorinated biphenyls in bed mud, its It is characterised by:Concentration of the sodium acetate in anode chamber is 10mM.
5. the method that a kind of utilization microorganism electrolysis cell device according to claim 1 removes Polychlorinated biphenyls in bed mud, its It is characterised by:Described hybrid electrochemical active bacteria is aerobic activated sludge in double-chamber microbiological electrolyzer, is applying current potential Stimulate and keep concentration in anode room for 10mM sodium acetate as electron donor in the case of tame and obtain;The perchlorate Domestication bacterium is anaerobic activated sludge, using sulphur powder as electron donor, in the case of improving the concentration of substrate perchlorate, is carried out Obtain after taming step by step.
6. the method that a kind of utilization microorganism electrolysis cell device according to claim 1 removes Polychlorinated biphenyls in bed mud, its It is characterised by:The pH is that 7.0 phosphate buffer includes 22.2g/L Na with the composition of the mixed liquor of nutritional solution2HPO4· 12H2O、5.92g/L NaH2PO4·2H2O、5.88g/L NaCl、1.0g/LNaHCO3、0.10g/L FeSO4、0.10g/L KCl、0.015g/L CaCl2、0.25g/L NH4Cl, 10mL/L mineral solution and 10mL/L vitamin solution;The perchloric acid The composition of salt domestication basic culture solution includes 0.41g/L NH4HCO3, 0.25g/L K2HPO4, 2.70g/L NaHCO3, 0.005g/ L Ca(OH)2, 10mL/L vitamins solution and 10mL/L mineral solutions.
7. the method that a kind of utilization microorganism electrolysis cell device according to claim 6 removes Polychlorinated biphenyls in bed mud, its It is characterised by:The mineral solution composition includes 1.5g/L C6H6NO6·3Na·12H2O、0.13g/L ZnCl2、3.0g/L MgSO4、0.01g/L CuSO4·5H2O、0.5g/L MnSO4·H2O、0.01g/L AlK(SO4)2·12H2O、1.0g/L NaCl、0.01g/L H3BO3、0.1g/L FeSO4·7H2O、0.025g/L Na2MoO4、0.1g/L CaCl2·2H2O、 0.024g/L NiCl2·6H2O、0.1g/L CoCl2·6H2O and 0.025g/L Na2WO4·2H2O;Described vitamin solution Composition includes 2.0mg/L biotin, 2.0mg/L Folic Acid, 10.0mg/L vitamin Bies, 5.0mg/L thiamines, 5.0mg/L core yellows Element, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenates, 0.1mg/L Vitamin B12, the acid of 5.0mg/L para-aminotoluenes and 5.0mg/L sulfur Octanoic acid.
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