CN104984994A - Device and method for removing polychlorinated biphenyl in bottom mud through microorganism electrolytic tank - Google Patents
Device and method for removing polychlorinated biphenyl in bottom mud through microorganism electrolytic tank Download PDFInfo
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Abstract
The invention belongs to the technical field of soil restoration and discloses a device and method for removing polychlorinated biphenyl in bottom mud through a microorganism electrolytic tank. The microorganism electrolytic tank is established, hybrid electrochemical activity bacteria and perchlorate domesticated bacteria are inoculated to an anode chamber, soil polluted by polychlorinated biphenyl is added, a nutrient solution is supplemented to the rest part, the water phase-bottom mud anode chamber is formed, anode potential is controlled to be kept constant through an electrochemical work station, growth and propagation of the hybrid electrochemical activity bacteria and the perchlorate domesticated bacteria can be promoted, dechloridation efficiency can be greatly improved, and constant bioelectric currents can be generated. By the adoption of the device and method, natural restoration and toxicity reduction can be conducted on the polluted soil, and good environmental protection benefits are achieved.
Description
Technical field
The invention belongs to technical field of soil remediation, be specifically related to a kind of device and method utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud.
Background technology
Polychlorinated biphenyls is widely applied in field of industrial production due to the excellent physicochemical property such as its thermodynamic stability, chemical inertness, low flammability, high conductivity, middle 1960s, whole world Polychlorinated biphenyls annual production is about 100,000 tons, from 20 century 70s, the Polychlorinated biphenyls annual production of China is close to ten thousand tons.As the typical persistence organic pollutant of one, the genotoxic potential of Polychlorinated biphenyls and structural stability bring serious harm to health and natural environment, and a kind of method of therefore seeking effective reparation polluted soil is extremely urgent.Minimizing technology at present for Polychlorinated biphenyls comprises traditional biodegrading process and emerging degradation technique, and traditional biodegrading process seals landfill method up for safekeeping, high temperature incineration method; Emerging degradation technique has supercritical water oxidation method, dissolves electronic technology, chemical reduction method, photochemical degradating method and microbial degradation method.The selection of Polychlorinated biphenyls minimizing technology depends primarily on its load configuration, kind and concentration value, wherein microbial degradation method is compared with other several class methods, have with low cost, easy and simple to handle, non-secondary pollution, possess the advantages such as in-situ immobilization meaning, simultaneously degradation rate is slow, need supplementary means to improve shortcoming place that Polychlorinated biphenyls biodegradability is also microbial degradation method.
In recent years, microbiological fuel cell technology is in wastewater treatment, desalination, the application prepared in biosensing apparatus reaches its maturity, and derivative bed mud-microbiological fuel cell technology promotes that the nature reparation of complicated organic matter such as phenol, phenanthrene, oil is also paid attention to gradually.
Therefore at saving running cost, under avoiding the prerequisite of secondary pollution, we utilize the mode of microorganism electrolysis cell, and strengthen reduction conversion and the oxidation removal of Polychlorinated biphenyls in contaminated soil, the based technique for in-situ remediation for Polychlorinated biphenyls provides new thinking.
Summary of the invention
In order to solve the shortcoming and defect part of prior art, primary and foremost purpose of the present invention is to provide a kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud.
Another object of the present invention is to provide a kind of method utilizing said apparatus to remove Polychlorinated biphenyls in bed mud.
The object of the invention is achieved through the following technical solutions:
A kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud, be made up of double-chamber microbiological electrolytic cell and multi-channel electrochemical work station, double-chamber microbiological electrolytic cell comprises anode chamber and cathode chamber, anode chamber and cathode chamber are separated by cation-exchange membrane, anode chamber arranges anode electrode and reference electrode, cathode chamber arranges cathode electrode, anode electrode, cathode electrode are communicated with multi-channel electrochemical work station with reference electrode, and the current potential that work station can control anode chamber keeps constant (relative reference electrode).
Described reference electrode is saturated calomel electrode, described anode electrode and the preferred carbon paper of the material of cathode electrode, graphite felt, carbon cloth; Anode is more preferably through pretreated carbon paper, and described preprocess method is, after carbon paper being rinsed oven dry by deionized water, soak 24h, subsequently heat treatment 12h at 100 DEG C under being placed in ethanol normal temperature; Carbon paper is after heat treatment placed in the mixed liquor of alkali lye and ethanol and soaks 24h, finally dries by rinsed with deionized water; Negative electrode is more preferably through pretreated graphite felt, and described preprocess method is that graphite felt to be immersed in mass fraction be in the hydrogen peroxide of 10%, poach 2h at 90 DEG C, and poach 2h after deionized water rinsing and in deionized water, takes out and dry.
Multi-channel electrochemical work station used is multichannel potentiostat.
Utilize said apparatus to remove a method for Polychlorinated biphenyls in bed mud, comprise following operating procedure:
The inoculation of anode room is through the hybrid electrochemical active bacteria of domestication, add sodium acetate as electron donor, be that the PBS of 7.0 and the mixed liquor of nutrient solution fill it up with anode chamber's remainder and cathode chamber with pH, observe that hybrid electrochemical active bacteria can continue electrogenesis, namely after stablizing biofilm, outwell the liquid in anode chamber, inoculation perchlorate domestication bacterium, then add the bed mud containing Polychlorinated biphenyls; Anode chamber's residual volume and cathode chamber all fill perchlorate domestication basic culture solution, and basic culture solution is through the nitrogen treatment removing dissolved oxygen wherein that exposes to the sun; Utilize multichannel potentiostat to be communicated with three electrodes of double-chamber microbiological electrolytic cell, set anode potential as+0.2V (relative to reference electrode), regular replenishment sodium acetate after commencement of commercial operation, completes the removal to Polychlorinated biphenyls in bed mud.
Described sodium acetate is preferably 10mM in the concentration of anode chamber.
Described hybrid electrochemical active bacteria be aerobic activated sludge in double-chamber microbiological electrolytic cell, when applying current potential and stimulate and the sodium acetate that the indoor concentration of holding anode is 10mM obtains as domestication when electron donor; Described perchlorate domestication bacterium is anaerobic activated sludge, using sulphur powder as electron donor, when improving the concentration of substrate perchlorate, obtains after taming step by step.
Described pH is that the composition of the phosphate buffer of 7.0 and the mixed liquor of nutrient solution comprises 22.2g/LNa
2hPO
412H
2o, 5.92g/L NaH
2pO
42H
2o, 5.88g/L NaCl, 1.0g/L NaHCO
3, 0.10g/L FeSO
4, 0.10g/L KCl, 0.015g/L CaCl
2, 0.25g/L NH
4cl, 10mL/L mineral solution and 10mL/L vitamin solution.
The composition of described perchlorate domestication basic culture solution comprises 0.41g/L NH
4hCO
3, 0.25g/LK
2hPO
4, 2.70g/L NaHCO
3, 0.005g/L Ca (OH)
2, 10mL/L vitamin solution and 10mL/L mineral solution.
Described mineral solution composition comprises 1.5g/L C
6h
6nO
63Na12H
2o, 0.13g/L ZnCl
2, 3.0g/L MgSO
4, 0.01g/L CuSO
45H
2o, 0.5g/L MnSO
4h
2o, 0.01g/LAlK (SO
4)
212H
2o, 1.0g/L NaCl, 0.01g/L H
3bO
3, 0.1g/L FeSO
47H
2o, 0.025g/LNa
2moO
4, 0.1g/L CaCl
22H
2o, 0.024g/L NiCl
26H
2o, 0.1g/L CoCl
26H
2o and 0.025g/L Na
2wO
42H
2o.
Described vitamin solution composition comprises 2.0mg/L biotin, 2.0mg/L folic acid, 10.0mg/L vitamin B6,5.0mg/L thiamine, 5.0mg/L riboflavin, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenate, 0.1mg/L vitamin B12, the acid of 5.0mg/L para-aminotoluene and 5.0mg/L lipoic acid.
Principle of the present invention is:
Build the microorganism electrolysis cell with anode chamber and cathode chamber, anode chamber is aqueous phase-bed mud (comprising electrode) system.Utilize the potential value of electrochemical workstation control anode chamber constant in+0.2V, the growth of carbon paper electrode surface electrochemistry active bacteria can be promoted and produce bioelectricity, the sodium acetate supplemented serves as electron donor and can maintain specificity dehalogenation bacterium and breathe the dehalogenation of Polychlorinated biphenyls, synergy is mutually there is between simultaneous electrochemical active bacteria and specificity dehalogenation bacterium, can promote electro transfer further and then realize the reduction dechlorination process of Polychlorinated biphenyls, and two class Pseudomonas can utilize organic matter abundant in load bed mud to meet own growth.
The material of anode electrode is preferably through pretreated carbon paper, carbon paper has higher specific area, good biocompatibility and reasonable prices, and can not the soil particle of absorption carriage Polychlorinated biphenyls, carbon paper is carried out pretreatment and be conducive to improving its stability, electric conductivity and biocompatibility.
Had the following advantages and beneficial effect by method of the present invention and device tool:
(1) the present invention adopts microorganism electrolysis cell to remove to the reduction realizing Polychlorinated biphenyls in bed mud, can significantly realize pollution amelioration, toxicity reduction on the one hand, it is with high costs that the problem of secondary pollution conventional art can being avoided to bring on the other hand and emerging technology are brought, the problem that operation easier is large.
(2) the present invention adopt hybrid electrochemical active bacteria and perchlorate domestication bacterium be under specified conditions, with specific nutrition liquid carry out domestication cultivation, there is efficient electricity generation ability and efficient dehalogenation ability;
(3) device and method of the present invention is except being applied in the reduction removal of Polychlorinated biphenyls, can also for the pollution amelioration of such as chlorobenzene, the PBDE etc. of other halogenated organic matters;
(4) the present invention's bed mud used can be taken from exemplary electronic rubbish and disassembles stream near field, wherein contain a large amount of high concentration cl after testing for complicated organic matter, comprise Polychlorinated biphenyls, therefore method and apparatus of the present invention can be applied in the in-situ immobilization of actual contaminated soil.
Accompanying drawing explanation
Fig. 1 is the apparatus structure schematic diagram that embodiment 1 a kind of utilizes microbiological fuel cell degradation of phenol and ammonia nitrogen simultaneously, in figure, mark is described as follows: 1-anode chamber, 2-cathode chamber, 3-cation-exchange membrane, 4-electrochemical workstation, 5-anode filling opening, 6-anode electrode, 7-reference electrode, 8-negative electrode filling opening, 9-cathode electrode, 10-external circuit;
Fig. 2 is Polychlorinated biphenyls (2,3,4,5-tetrachloro biphenyl, PCB61) concentration curve over time in embodiment 1 ~ 2 and comparative example 1 ~ 3;
Fig. 3 is Polychlorinated biphenyls intermediates 2,3,5-trichloro biphenyl (PCB23) and 2,4,5-trichloro biphenyl (PCB29) concentration curve over time in embodiment 1 ~ 2 and comparative example 1 ~ 3;
Fig. 4 is embodiment 1 and current strength curve over time in embodiment 2.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
The hybrid electrochemical active bacteria used in following examples and perchlorate domestication bacterium are screened by the following method and tame and obtain:
Take from the activated sludge 10mL of Shaoguan City of Guangdong Province Shaoguan Iron & Steel Plant coking chemical waste water second phase process engineering O1 section, join the anode of double-chamber microbiological electrolytic cell, wherein the capacity of battery is 200mL, anode chamber and each 100mL of cathode chamber; Add 1mL in anode chamber, concentration be the sodium acetate of 1M as electron donor, anode chamber's remainder and cathode chamber fill it up with the phosphate buffer solution of pH=7.0 and the mixed liquor of nutrient solution, installation code saturated calomel reference electrode, formed three-electrode system; The above-mentioned battery assembled is put into 30 DEG C of constant temperature and humidity incubators, galvanic anode, negative electrode, reference electrode connect respectively electrochemical workstation working electrode line, to electrode wires and reference electrode wire, and impose the current potential of 0.2V vs SCE, changed fresh medium every five days and supplement same volume, sodium acetate with concentration; After one month, after adding sodium acetate in microbiological fuel cell battery, to respond fast and bioelectricity can reach 10mA-20mA, tame successfully, obtain hybrid electrochemical active bacteria.
The anaerobism section activated sludge taking from Guangzhou, Guangdong drop Kau municipal sewage plant is about 200ml, be placed in the indigo plant lid bottle that volume is 1L, remainder supplement basic culture solution and the perchlorate adding 10g sublimed sulfur powder and 10mg/L as electron donor and electron acceptor, the nitrogen that exposes to the sun is driven oxygen away and is placed in the shaking table being set as 150 revs/min, 30 DEG C; Regularly (5-10 days) leaves standstill and changes culture supernatants and the concentration of adding perchlorate according to perchlorate reduction's effect by gradient further, after trimestral adaptation is cultivated, the perchlorate of 100mg/L can be reduced into chlorion in two days, tame successfully, obtain perchlorate domestication bacterium.
The load bed mud used in following examples take from Qingyuan City Long Tang town, Guangdong Province electronic waste disassemble factory (north latitude 23 ° of 36'12.30 "; the bed mud of river below cement interface 10cm near east longitude 113 ° of 04'38.84 "), the mud got removes the wherein impurity such as branch, leaf, stone through screening, preserves under normal temperature.
Embodiment 1
As shown in Figure 1, a kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud of the present embodiment, be made up of double-chamber microbiological electrolytic cell and electrochemical workstation, double-chamber microbiological electrolytic cell comprises anode chamber 1 and cathode chamber 2, anode chamber and cathode chamber are separated by cation-exchange membrane 3, anode chamber arranges galvanic anode filling opening 5, anode electrode 6 and reference electrode 7, cathode chamber arranges cell cathode filling opening 8 and cathode electrode 9, anode electrode, reference electrode is connected with electrochemical workstation 4 by external circuit 10 with cathode electrode, electrochemical workstation constant control anode keeps the current potential of+0.2V.
The device of the present embodiment is for removing the method for Polychlorinated biphenyls in bed mud, concrete steps are: inoculation 10ml in anode room is through the hybrid electrochemical active bacteria liquid of domestication, adding 1ml concentration is that the sodium acetate of 1M is as electron donor, be that the PBS of 7.0 and the mixed liquor of nutrient solution fill it up with anode chamber's remainder and cathode chamber with pH, observe that electrogenesis bacterium can continue electrogenesis, namely after stablizing biofilm, outwell the liquid in anode chamber, add the perchlorate domestication bacterium liquid of 30ml, and using the bed mud taking from Qingyuan City as load bed mud, control Polychlorinated biphenyls (2, 3, 4, 5-tetrachloro biphenyl, PCB61) concentration is 100uM/kg, anode chamber's remainder and cathode chamber all fill perchlorate domestication basic culture solution, carbon paper working electrode, the saturated calomel reference electrode of anode chamber are connected with the corresponding joints of electrochemical workstation (Shanghai Chen Hua Instrument Ltd. produces CHI1000C type eight passage potentiostat) respectively to electrode with the graphite carbon felt of cathode chamber, and apply the potential value of+0.2V vs SCE, after self-starting, every 20 days supplementary 1ml, concentration is the sodium acetate of 1M.
The carbon paper working electrode of the present embodiment is before use through following methods pretreatment: after carbon paper being rinsed oven dry by deionized water, soak 24h, subsequently heat treatment 12h at 100 DEG C under being placed in ethanol normal temperature; Carbon paper after heat treatment is placed in the mixed liquor of alkali lye (0.3M) and ethanol and soaks 24h, finally dries by rinsed with deionized water; Graphite carbon felt to electrode before use through following methods pretreatment: graphite felt being immersed in mass fraction is in the hydrogen peroxide of 10%, poach 2h at 90 DEG C, poach 2h after deionized water rinsing and in deionized water, takes out and dries.
Described pH is that the PBS of 7.0 and the mixed liquor composition of nutrient solution comprise 22.2g/LNa
2hPO
4, 5.92g/L NaH
2pO
4, 1.0g/L NaHCO
3, 0.10g/L FeSO
4, 0.10g/L KCl, 0.015g/L CaCl
2, 0.25g/LNH
4cl, 10mL/L mineral solution and 10mL/L vitamin; Perchlorate domestication basic culture solution composition comprises 0.41g/L NH
4hCO
3, 0.25g/L K
2hPO
4, 2.70g/LNaHCO
3, 0.005g/L Ca (OH)
2, 10mL/L vitamin solution and 10mL/L mineral solution; Vitamin solution composition is: 2.0mg/L biotin, 2.0mg/L folic acid, 10.0mg/L vitamin B6,5.0mg/L thiamine, 5.0mg/L riboflavin, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenate, 0.1mg/L vitamin B12, the acid of 5.0mg/L para-aminotoluene, 5.0mg/L lipoic acid; Mineral solution composition is: 1.5g/L C
6h
6nO
63Na12H
2o, 0.13g/L ZnCl
2, 3.0g/L MgSO4,0.01g/L CuSO
45H
2o, 0.5g/L MnSO
4h
2o, 0.01g/L AlK (SO
4)
212H
2o, 1.0g/L NaCl, 0.01g/L H
3bO
3, 0.1g/L FeSO
47H
2o, 0.025g/L Na
2moO
4, 0.1g/L CaCl
22H
2o, 0.024g/LNiCl
26H
2o, 0.1g/L CoCl
26H
2o and 0.025g/L Na
2wO
42H
2o.
Embodiment 2
The present embodiment a kind of utilizes microorganism electrolysis cell to remove the apparatus and method of Polychlorinated biphenyls in bed mud, and compared with embodiment 1, difference is not have regular replenishment 1ml concentration to be the sodium acetate of 1M, and remainder is identical.
Comparative example 1
A kind of apparatus and method utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud of this comparative example, compared with embodiment 1, difference is that the electrode of the anode chamber and the cathode chamber is not connected with electrochemical workstation, and remainder is identical.
Comparative example 2
A kind of apparatus and method utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud of this comparative example, compared with embodiment 2, difference is that the electrode of the anode chamber and the cathode chamber is not connected with electrochemical workstation, and remainder is identical.
Comparative example 3
A kind of apparatus and method utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls of this comparative example, compared with embodiment 2, difference is not inoculate hybrid electrochemical active bacteria and perchlorate domestication bacterium, load bed mud through sterilization treatment and the electrode of the anode chamber and the cathode chamber be not connected with electrochemical workstation, all the other operations are identical.
Device and method in above-described embodiment and comparative example is used for the effectiveness comparison that in bed mud, Polychlorinated biphenyls is removed:
In embodiment 1 ~ 2 and comparative example 1 ~ 3, curve is as shown in Figure 2 over time for tetrachloro biphenyl PCB61 concentration; Curve is as shown in Figure 3 over time for Polychlorinated biphenyls intermediates trichloro biphenyl PCB23 and PCB29 concentration.As can be seen from Fig. 2 and Fig. 3: in (comparative example 3) reactor that sterilization treatment is crossed the concentration of target Polychlorinated biphenyls PCB61 do not observe minimizing and catabolite does not observe generation yet; And inoculate in the reactor of hybrid electrochemical active bacteria and perchlorate domestication bacterium liquid, wherein do not connect electrochemical workstation and non-supplementary quota exoelectron donor sodium acetate when (comparative example 2), initial concentration is 100 μm of ol kg
-1pCB61 in 22.6 μm of ol kg
-1generation reduction conversion, does not connect electrochemical workstation but supplementary quota exoelectron donor sodium acetate then has 35.8 μm of ol kg
-1there is reduction and transform (comparative example 1); On this basis, by electrochemical workstation apply with+0.2V constant potential stimulate reactor in, wherein connect electrochemical workstation but non-supplementary quota exoelectron donor sodium acetate when (embodiment 2), initial concentration is 100 μm of ol kg
-1pCB61 in 57.7 μm of ol kg
-1generation reduction conversion, connects electrochemical workstation simultaneously and supplementary quota exoelectron donor sodium acetate (embodiment 1) then has 78.2 μm of ol kg
-1there is reduction to transform.This illustrates that standing in anode by electrochemical operation applies the current potential of+0.2V and regular replenishment electron donor sodium acetate has great effect to maintain its concentration to hybrid electrochemical active bacteria and perchlorate domestication bacterium decomposition and inversion Polychlorinated biphenyls.In addition as can be seen from Fig. 3 also: the generation of PCB23 is apparently higher than PCB29, and the dechlorination of Polychlorinated biphenyls PCB61 mainly occurs in contraposition and a position, corresponding dechlorination product is respectively PCB23 and PCB29, therefore hybrid electrochemical active bacteria of the present invention and perchlorate domestication bacterium are based on the contraposition dechlorination to Polychlorinated biphenyls.
Embodiment 1 and current strength in embodiment 2 over time curve respectively as shown in (a) He (b) in Fig. 4.As can be seen from (a) in Fig. 4 and (b): work station monitors the change of current density Development pattern wherein, in 100 days of reaction generation, in embodiment 1, current density is from the 0.29mA cm of initial one-period
-2be down to the 0.19mA cm in the tenth cycle
-2then continue to be reduced to 0.12mA cm
-2; And current density in embodiment 2 is from the 0.031mA cm of initial one-period
-2be down to the 0.022mA cm in the tenth cycle
-2then continue to be reduced to 0.010mA cm
-2.
Learnt by above result, when abundant hybrid electrochemical active bacteria and perchlorate domestication bacterium, a small amount of reduction dechlorination process can be there is in Polychlorinated biphenyls, and the already present organic matter of load bed mud can be utilized to meet the demand of dehalogenation breathing to electron donor, and by electrochemical workstation, constant current potential stimulation is applied to reaction system, the growth of hybrid electrochemical active bacteria can not only be strengthened and produce bioelectricity, also can promote the growth of relevant dehalogenation bacterium (perchlorate domestication bacterium) simultaneously and strengthen dehalogenation process.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (9)
1. the device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud, it is characterized in that: described device is made up of double-chamber microbiological electrolytic cell and multi-channel electrochemical work station, described double-chamber microbiological electrolytic cell comprises anode chamber and cathode chamber, anode chamber and cathode chamber are separated by cation-exchange membrane, anode chamber arranges anode electrode and reference electrode, and cathode chamber arranges cathode electrode; Anode electrode, cathode electrode are communicated with multi-channel electrochemical work station with reference electrode, and the current potential that work station controls anode chamber keeps constant.
2. a kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud according to claim 1, it is characterized in that: described reference electrode is saturated calomel electrode, described anode electrode and the material of cathode electrode are carbon paper, graphite felt or carbon cloth.
3. a kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud according to claim 2, it is characterized in that: the material of described anode electrode is through pretreated carbon paper, described preprocess method is after carbon paper being rinsed oven dry by deionized water, 24h is soaked under being placed in ethanol normal temperature, heat treatment 12h at 100 DEG C subsequently, carbon paper is after heat treatment placed in the mixed liquor of alkali lye and ethanol and soaks 24h, finally dries by rinsed with deionized water; The material of described cathode electrode is through pretreated graphite felt, and described preprocess method is that graphite felt to be immersed in mass fraction be in the hydrogen peroxide of 10%, poach 2h at 90 DEG C, and poach 2h after deionized water rinsing and in deionized water, takes out and dry.
4. a kind of device utilizing microorganism electrolysis cell to remove Polychlorinated biphenyls in bed mud according to any one of claims 1 to 3, is characterized in that: described multi-channel electrochemical work station is multichannel potentiostat.
5. utilize the device described in claim 4 to remove a method for Polychlorinated biphenyls in bed mud, it is characterized in that comprising following operating procedure:
The inoculation of anode room is through the hybrid electrochemical active bacteria of domestication, add sodium acetate as electron donor, be that the PBS of 7.0 and the mixed liquor of nutrient solution fill it up with anode chamber's remainder and cathode chamber with pH, observe that hybrid electrochemical active bacteria can continue electrogenesis, namely after stablizing biofilm, outwell the liquid in anode chamber, inoculation perchlorate domestication bacterium, then add the bed mud containing Polychlorinated biphenyls; Anode chamber's residual volume and cathode chamber all fill perchlorate domestication basic culture solution, and basic culture solution is through the nitrogen treatment removing dissolved oxygen wherein that exposes to the sun; Utilize multichannel potentiostat to be communicated with three electrodes of double-chamber microbiological electrolytic cell, set anode potential as+0.2V, regular replenishment sodium acetate after commencement of commercial operation, completes the removal to Polychlorinated biphenyls in bed mud.
6. the method for Polychlorinated biphenyls in removal bed mud according to claim 5, is characterized in that: the concentration of described sodium acetate in anode chamber is 10mM.
7. the method for Polychlorinated biphenyls in removal bed mud according to claim 5, it is characterized in that: described hybrid electrochemical active bacteria be aerobic activated sludge in double-chamber microbiological electrolytic cell, when applying current potential and stimulate and the sodium acetate that the indoor concentration of holding anode is 10mM obtains as domestication when electron donor; Described perchlorate domestication bacterium is anaerobic activated sludge, using sulphur powder as electron donor, when improving the concentration of substrate perchlorate, obtains after taming step by step.
8. the method for Polychlorinated biphenyls in removal bed mud according to claim 5, is characterized in that: described pH is that the composition of the phosphate buffer of 7.0 and the mixed liquor of nutrient solution comprises 22.2g/LNa
2hPO
412H
2o, 5.92g/L NaH
2pO
42H
2o, 5.88g/L NaCl, 1.0g/L NaHCO
3, 0.10g/L FeSO
4, 0.10g/L KCl, 0.015g/L CaCl
2, 0.25g/L NH
4cl, 10mL/L mineral solution and 10mL/L vitamin solution; The composition of described perchlorate domestication basic culture solution comprises 0.41g/LNH
4hCO
3, 0.25g/L K
2hPO
4, 2.70g/L NaHCO
3, 0.005g/L Ca (OH)
2, 10mL/L vitamin solution and 10mL/L mineral solution.
9. the method for Polychlorinated biphenyls in removal bed mud according to claim 8, is characterized in that: described mineral solution composition comprises 1.5g/L C
6h
6nO
63Na12H
2o, 0.13g/L ZnCl
2, 3.0g/LMgSO
4, 0.01g/L CuSO
45H
2o, 0.5g/L MnSO
4h
2o, 0.01g/L AlK (SO
4)
212H
2o, 1.0g/L NaCl, 0.01g/L H
3bO
3, 0.1g/L FeSO
47H
2o, 0.025g/L Na
2moO
4, 0.1g/LCaCl
22H
2o, 0.024g/L NiCl
26H
2o, 0.1g/L CoCl
26H
2o and 0.025g/LNa
2wO
42H
2o; Described vitamin solution composition comprises 2.0mg/L biotin, 2.0mg/L folic acid, 10.0mg/L vitamin B6,5.0mg/L thiamine, 5.0mg/L riboflavin, 5.0mg/L nicotinic acid, 5.0mg/L calcium pantothenate, 0.1mg/L vitamin B12, the acid of 5.0mg/L para-aminotoluene and 5.0mg/L lipoic acid.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105817476A (en) * | 2016-03-24 | 2016-08-03 | 华南理工大学 | Degradation reinforcing method for polychlorinated biphenyl in soil or sediment |
| CN106048647A (en) * | 2016-07-01 | 2016-10-26 | 吉林建筑大学 | Variable-polarity biological electrode microorganism electrolytic tank and running method thereof |
| CN106734158A (en) * | 2016-11-24 | 2017-05-31 | 华南理工大学 | A kind of electric microfield stimulates the aerobic method and apparatus for realizing Polychlorinated biphenyls removal in bed mud of coupling negative electrode anaerobism/anode |
| CN108380656A (en) * | 2018-01-25 | 2018-08-10 | 中国石油大学(北京) | The processing unit of one heavy metal species soil and its application |
| CN108569757A (en) * | 2018-05-09 | 2018-09-25 | 南开大学 | A method of handling sulfamido waste water and synchronous hydrogen manufacturing using electrochemical appliance |
| CN108817076A (en) * | 2018-06-08 | 2018-11-16 | 广西博世科环保科技股份有限公司 | The method and system of microorganism electrochemical respirator reinforcing remedying oil-polluted soils |
| CN110282839A (en) * | 2019-06-10 | 2019-09-27 | 中交四航工程研究院有限公司 | The method for realizing halogenated organic pollutant dehalogenation in bed mud is coupled using microorganism electrochemical |
| CN109133550B (en) * | 2018-09-09 | 2021-03-26 | 南京工业大学 | Method for producing methane and synchronously degrading phenanthrene through anaerobic digestion of sludge |
| CN112694198A (en) * | 2020-11-27 | 2021-04-23 | 浙江工业大学 | Method for dechlorinating trichloroacetic acid through catalytic electrolysis of vitamin B12 modified electrode |
| CN113087124A (en) * | 2021-04-14 | 2021-07-09 | 东南大学 | Bioelectrochemical device for synchronously degrading chlorophenol in water phase by using cathode and anode |
| CN113398523A (en) * | 2021-05-12 | 2021-09-17 | 华南理工大学 | FeSxElectrode, preparation method and FeSxDevice and method for fixing Cr (VI) through synergistic microbial mineralization |
| CN113582328A (en) * | 2021-08-20 | 2021-11-02 | 浙江大学 | Method for strengthening anaerobic dechlorination of polychlorinated biphenyl microorganisms |
| CN114433619A (en) * | 2022-02-18 | 2022-05-06 | 南开大学 | Self-assembled electrode for soil carbon emission reduction and application thereof |
| CN114951259A (en) * | 2022-06-06 | 2022-08-30 | 东北农业大学 | Method for repairing lead and atrazine polluted soil by double-membrane electric system coupling functional microbial inoculum |
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| CN105817476A (en) * | 2016-03-24 | 2016-08-03 | 华南理工大学 | Degradation reinforcing method for polychlorinated biphenyl in soil or sediment |
| CN105817476B (en) * | 2016-03-24 | 2020-04-24 | 华南理工大学 | Degradation strengthening method for polychlorinated biphenyl in soil or sediment |
| CN106048647A (en) * | 2016-07-01 | 2016-10-26 | 吉林建筑大学 | Variable-polarity biological electrode microorganism electrolytic tank and running method thereof |
| CN106734158A (en) * | 2016-11-24 | 2017-05-31 | 华南理工大学 | A kind of electric microfield stimulates the aerobic method and apparatus for realizing Polychlorinated biphenyls removal in bed mud of coupling negative electrode anaerobism/anode |
| CN106734158B (en) * | 2016-11-24 | 2020-05-22 | 华南理工大学 | Method and device for removing polychlorinated biphenyl in bottom sludge by virtue of micro-electric field stimulation coupled with cathode anaerobic/anode aerobic |
| CN108380656A (en) * | 2018-01-25 | 2018-08-10 | 中国石油大学(北京) | The processing unit of one heavy metal species soil and its application |
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| CN108817076A (en) * | 2018-06-08 | 2018-11-16 | 广西博世科环保科技股份有限公司 | The method and system of microorganism electrochemical respirator reinforcing remedying oil-polluted soils |
| CN109133550B (en) * | 2018-09-09 | 2021-03-26 | 南京工业大学 | Method for producing methane and synchronously degrading phenanthrene through anaerobic digestion of sludge |
| CN110282839A (en) * | 2019-06-10 | 2019-09-27 | 中交四航工程研究院有限公司 | The method for realizing halogenated organic pollutant dehalogenation in bed mud is coupled using microorganism electrochemical |
| CN112694198A (en) * | 2020-11-27 | 2021-04-23 | 浙江工业大学 | Method for dechlorinating trichloroacetic acid through catalytic electrolysis of vitamin B12 modified electrode |
| CN112694198B (en) * | 2020-11-27 | 2022-06-17 | 浙江工业大学 | Method for dechlorinating trichloroacetic acid through catalytic electrolysis of vitamin B12 modified electrode |
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| CN113087124B (en) * | 2021-04-14 | 2022-11-04 | 东南大学 | Bioelectrochemical device for synchronously degrading chlorophenol in water phase by using cathode and anode |
| CN113398523A (en) * | 2021-05-12 | 2021-09-17 | 华南理工大学 | FeSxElectrode, preparation method and FeSxDevice and method for fixing Cr (VI) through synergistic microbial mineralization |
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| CN113582328A (en) * | 2021-08-20 | 2021-11-02 | 浙江大学 | Method for strengthening anaerobic dechlorination of polychlorinated biphenyl microorganisms |
| CN114433619A (en) * | 2022-02-18 | 2022-05-06 | 南开大学 | Self-assembled electrode for soil carbon emission reduction and application thereof |
| CN114951259A (en) * | 2022-06-06 | 2022-08-30 | 东北农业大学 | Method for repairing lead and atrazine polluted soil by double-membrane electric system coupling functional microbial inoculum |
| CN114951259B (en) * | 2022-06-06 | 2024-01-16 | 东北农业大学 | Method for repairing lead and atrazine polluted soil by coupling functional microbial inoculum of double-membrane electric system |
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