CN106399090B - Electromagnetic environment generator and experimental method for microculture test - Google Patents
Electromagnetic environment generator and experimental method for microculture test Download PDFInfo
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- CN106399090B CN106399090B CN201610808494.5A CN201610808494A CN106399090B CN 106399090 B CN106399090 B CN 106399090B CN 201610808494 A CN201610808494 A CN 201610808494A CN 106399090 B CN106399090 B CN 106399090B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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Abstract
The invention discloses a kind of electromagnetic environment generators and experimental method for microculture test, belong to bioelectromagnetics field.The electromagnetic environment generator for microculture test includes iron core, single-chip microcontroller, power supply and a pair of electrodes plate, in which: the coil of certain the number of turns is wound in the middle part of iron core, coil is connected to power supply through the first pressure regulator；A pair of electrodes plate is placed in after iron core is powered and is formed by between the pole N and the pole S, and a pair of electrodes plate is connected to power supply through the second pressure regulator；Single-chip microcontroller is connect with the first pressure regulator with the second pressure regulator respectively, and single-chip microcontroller is connected with display.The present invention can for power frequency electromagnetic field to microorganism influence research foundation is provided and provides directive function to the research of microorganism for power frequency electromagnetic field.
Description
Technical field
The present invention relates to bioelectromagnetics fields, particularly relate to a kind of electromagnetic environment generation for microculture test
Device and experimental method.
Background technique
Power frequency electromagnetic field refers to electromagnetic field caused by voltage and current of the period for 50Hz.In daily life, various electric appliances
Such as television set, hair dryer, computer can generate power frequency electromagnetic field.The power frequency electromagnetic field that these common household electrical appliances generate can be to biology
Distribution of charges, arrangement and the movement of body itself have an impact.In order to study electromagnetic field to the active influence of organism, it is fixed to need
The electromagnetic environment of amount, to facilitate under the various electromagnetic environments of research, influence suffered by organism.
The influence to health and environment is focused primarily upon for the research of power frequency electromagnetic field at present.There is part research shows that work
The exposure of frequency electromagnetic field may be related with the illness of systems such as the morbidity of tumour, nerve, angiocarpy, endocrine, reproduction, also grinds
Study carefully discovery will lead to the reproductive system disorder of rat after power frequency electromagnetic field exposed amount reaches certain time, some strength, also have
Part is research shows that power frequency electromagnetic field exposure will not have an impact public health.And the spy of bacterium is killed for power frequency electromagnetic field
Study carefully seldom and without final conclusion.
It is surveyed therefore, it is necessary to which the influence for research power frequency electromagnet field intensity to microorganism provides one kind for microculture
The device of examination.
Summary of the invention
The present invention provide it is a kind of can the influence for power frequency electromagnetic field to microorganism study and foundation is provided and is power frequency electric
Magnetic field provides the electromagnetic environment generator and experimental method for microculture test of guidance to the research of microorganism.
In order to solve the above technical problems, present invention offer technical solution is as follows:
On the one hand, the present invention provides a kind of electromagnetic environment generator for microculture test, including iron core, monolithic
Mechanical, electrical source and a pair of electrodes plate, in which:
The coil of certain the number of turns is wound in the middle part of the iron core, the coil connects through the first pressure regulator and the power supply
It connects；
The pair of electrode plate is placed in after the iron core is powered and is formed by between the pole N and the pole S, the pair of electrode plate
It is connect through the second pressure regulator with the power supply；
The single-chip microcontroller is connect with first pressure regulator with the second pressure regulator respectively, and the single-chip microcontroller is connected with display
Device.
Further, the iron core is c-type, the winding centerline parallel of the pair of electrode plate and the coil.
Further, the iron core is ferromagnetic material.
Further, the electromagnetic environment generator further includes cabinet, and the cabinet includes several laterally disposed interlayers.
Further, the cabinet is internally provided with temp and humidity regulator.
Further, the top of the interlayer is provided with the partition vertically placed.
On the other hand, the present invention also provides a kind of realities of above-mentioned electromagnetic environment generator for microculture test
Proved recipe method, comprising:
Step 1: in each experimental group, the iron core and a pair of electrodes plate being placed on the first pallet, the first culture dish
It is placed between the both ends of the iron core and the pair of electrode plate；
Step 2: in each control group, the second culture dish being placed on the second pallet；
Step 3: the experimental group and control group being placed in a constant temperature and humidity incubator, first pressure regulator is passed through
Different electromagnetic field intensities is respectively set with the second pressure regulator；
Step 4: after processing a period of time, the experimental group and control group being compared.
Wherein, in the step 1, the quantity of the experimental group is 2-3 group, and every group of quantity of the experimental group is 2-3
A, in the step 2, the quantity of the control group is 2-3, in the step 1 and step 2, the experimental group and control group
In the quantity of culture dish be 1.
Wherein, in the step 3, the control group is placed in the top layer of the constant temperature and humidity incubator, the experimental group
It is placed in the second to four layer of the constant temperature and humidity incubator.
The invention has the following advantages:
Of the invention electromagnetic environment generator and experimental method for microculture test, in use, by iron core and
A pair of electrodes plate is placed on independent pallet, and culture dish is placed between the both ends of iron core and a pair of electrodes plate, each control
Pallet on the culture dish holding of group, the influence that test electromagnetic field environment breeds it.According to experiment needs, each experimental group is logical
It crosses pressure regulator and different electromagnetic field intensities is respectively set.After handling a period of time, each experimental group and control group are carried out respectively
Comparison.Electromagnetic environment generator for microculture test of the invention, can be with various in simulating natural environment
Magnetic distribution generates the adjustable electromagnetic field of even intensity, and utilizes homemade electromagnetic environment generator, can study power frequency
Influence of the electromagnetic field to microorganism, the influence research for power frequency electromagnetic field to microorganism provide foundation.
Detailed description of the invention
Fig. 1 is the systematic schematic diagram of the electromagnetic environment generator for microculture test of the invention；
Fig. 2 is the overall structure diagram of the electromagnetic environment generator for microculture test of the invention；
Fig. 3 is the experimental group signal of the experimental method of the electromagnetic environment generator for microculture test of the invention
Figure；
Fig. 4 is the control group signal of the experimental method of the electromagnetic environment generator for microculture test of the invention
Figure.
Specific embodiment
To keep the technical problem to be solved in the present invention, technical solution and advantage clearer, below in conjunction with attached drawing and tool
Body embodiment is described in detail.
On the one hand, the present invention provides a kind of electromagnetic environment generator for microculture test, as shown in Figs 1-4,
Including iron core 1, single-chip microcontroller 2, power supply 3 and a pair of electrodes plate 4, in which:
The middle part of iron core 1 is wound with the coil 5 of certain the number of turns, and coil 5 is connect through the first pressure regulator 6 with power supply 3；
A pair of electrodes plate 4 is placed in after iron core 1 is powered and is formed by between the pole N and the pole S, and a pair of electrodes plate 4 is adjusted through second
Depressor 7 is connect with power supply 3；
Single-chip microcontroller 2 is connect with the first pressure regulator 6 with the second pressure regulator 7 respectively, and single-chip microcontroller 2 is connected with display 8.
Electromagnetic environment generator for microculture test of the invention, in use, by iron core and a pair of electrodes plate
It is placed on independent pallet, culture dish is placed between the both ends of iron core and a pair of electrodes plate, on the pallet of each control group
Culture dish holding, the influence that test electromagnetic field environment breeds it.According to experiment needs, each experimental group passes through pressure regulator point
Different electromagnetic field intensities is not set.After handling a period of time, each experimental group and control group are compared respectively.The present invention
For microculture test electromagnetic environment generator, can with magnetic distributions various in simulating natural environment,
The adjustable electromagnetic field of even intensity is generated, and utilizes homemade electromagnetic environment generator, power frequency electromagnetic field can be studied to micro-
The influence of biology, the influence research for power frequency electromagnetic field to microorganism provide foundation.
Preferably, iron core 1 is c-type, the winding centerline parallel of a pair of electrodes plate 4 and coil 5.Iron core can be prevented in this way
When generating stronger magnetic field, electrode plate is not breakdown.
Further, iron core 1 is ferromagnetic material.In this way when coil is powered, biggish magnetic induction intensity can produce.
As an improvement of the present invention, electromagnetic environment generator further includes cabinet 9, and cabinet 9 includes several laterally disposed
Interlayer 10, the top of interlayer 10 is provided with the partition 11 vertically placed, and partition 11 is preferably the partition with through-hole, and through-hole can be with
For arbitrary shape.This structure can place multiple groups experimental group and control group, can also make between each experimental group and control group
Keep identical temperature and humidity.
For the ease of the control to experimental situation, cabinet 9 is internally provided with temp and humidity regulator.
On the other hand, the present invention also provides a kind of realities of above-mentioned electromagnetic environment generator for microculture test
Proved recipe method, comprising:
Step 1: as shown in figure 3, iron core 1 and a pair of electrodes plate 4 are placed on the first pallet 12 in each experimental group,
First culture dish 13 is placed between the both ends of iron core 1 and a pair of electrodes plate 4；
Step 2: as shown in figure 4, the second culture dish 14 is placed on the second pallet 15 in each control group；
Step 3: experimental group and control group being placed in a constant temperature and humidity incubator, the first pressure regulator 6 and second is passed through
Different electromagnetic field intensities is respectively set in pressure regulator 7；
Step 4: after processing a period of time, experimental group and control group being compared.
The present invention uses homemade electromagnetic environment generator, can study influence of the power frequency electromagnetic field to microorganism, is work
Influence research of the frequency electromagnetic field to microorganism provides foundation.
Preferably, in step 1, the quantity of experimental group is 2-3 group, and every group of quantity of experimental group is 2-3, in step 2,
The quantity of control group is 2-3, and in step 1 and step 2, the quantity of the culture dish in experimental group and control group is 1.Setting
Multiple groups experimental group and control group can not only study influence of the different electromagnetic field intensities to microorganism simultaneously, can also reduce idol
Right influence of the factor to experimental result.
Further, in step 3, control group is placed in the top layer of constant temperature and humidity incubator, and experimental group is placed in constant temperature perseverance
The second to four layer of wet incubator.More easily experimental group and control group can be compared in this way, convenient for observation.
The above is a preferred embodiment of the present invention, it is noted that for those skilled in the art
For, without departing from the principles of the present invention, it can also make several improvements and retouch, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (7)
1. a kind of electromagnetic environment generator for microculture test, which is characterized in that including iron core, single-chip microcontroller, power supply
With a pair of electrodes plate, in which:
The coil of certain the number of turns is wound in the middle part of the iron core, the coil is connect through the first pressure regulator with the power supply；
The pair of electrode plate is placed in after the iron core is powered and is formed by between the pole N and the pole S, and the pair of electrode plate is through the
Two pressure regulators are connect with the power supply；
The single-chip microcontroller is connect with first pressure regulator with the second pressure regulator respectively, and the single-chip microcontroller is connected with display；
The iron core is c-type, and the indentation, there of c-type iron core, the pair of electrode plate and the line is arranged in the pair of electrode plate
The winding centerline parallel of circle；
The electromagnetic environment generator further includes cabinet, and the cabinet includes several laterally disposed interlayers.
2. the electromagnetic environment generator according to claim 1 for microculture test, which is characterized in that the iron
Core is ferromagnetic material.
3. the electromagnetic environment generator according to claim 2 for microculture test, which is characterized in that the case
Body is internally provided with temp and humidity regulator.
4. it is according to claim 2 for microculture test electromagnetic environment generator, which is characterized in that it is described every
The top of layer is provided with the partition vertically placed.
5. the experimental method of any electromagnetic environment generator for microculture test in claim 1-4,
It is characterized in that, comprising:
Step 1: in each experimental group, the iron core and a pair of electrodes plate being placed on the first pallet, the first culture dish is placed
Between the both ends and the pair of electrode plate of the iron core；
Step 2: in each control group, the second culture dish being placed on the second pallet；
Step 3: the experimental group and control group being placed in a constant temperature and humidity incubator, first pressure regulator and are passed through
Different electromagnetic field intensities is respectively set in two pressure regulators；
Step 4: after processing a period of time, the experimental group and control group being compared.
6. the experimental method of the electromagnetic environment generator according to claim 5 for microculture test, feature
It is, in the step 1, the quantity of the experimental group is 2-3 group, and every group of quantity of the experimental group is 2-3, the step
In rapid 2, the quantity of the control group is 2-3, the culture dish in the step 1 and step 2, in the experimental group and control group
Quantity be 1.
7. the experimental method of the electromagnetic environment generator according to claim 6 for microculture test, feature
It is, in the step 3, the control group is placed in the top layer of the constant temperature and humidity incubator, and the experimental group is placed in institute
The second to four layer for stating constant temperature and humidity incubator.
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| TWI703213B (en) * | 2019-03-28 | 2020-09-01 | 財團法人國家衛生研究院 | Cell culture device and electromagnetic cell culturing system thereof |
| CN112915939B (en) * | 2020-12-30 | 2022-06-10 | 广东电网有限责任公司电力科学研究院 | Quick aging reactor for transformer oil |
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| CN2051429U (en) * | 1989-06-22 | 1990-01-24 | 郭静峰 | High intensity magnetic field seed processing apparatus |
| CN201288186Y (en) * | 2008-06-26 | 2009-08-12 | 张首誉 | Multifunctional electromagnetic biological cultivation case |
| CN101463319A (en) * | 2009-01-07 | 2009-06-24 | 中国医学科学院生物医学工程研究所 | Apparatus for cell cultivation by pulse electromagnetic field |
| US9694193B2 (en) * | 2009-03-03 | 2017-07-04 | Technische Universiteit Eindhoven | Device and method for treating cells |
| CN202830045U (en) * | 2012-09-21 | 2013-03-27 | 关节动力安达（天津）生物科技有限公司 | Carbon dioxide cell incubator |
| CN104277970B (en) * | 2013-07-01 | 2016-02-24 | 中国科学院过程工程研究所 | The apparatus and method of a kind of electromagnetically induced electrogenesis bacteria distribution, growth and domestication |
| CN104238604A (en) * | 2014-09-23 | 2014-12-24 | 江苏农林职业技术学院 | Electromagnetic induction biological culture box and control method thereof |
| CN105761882A (en) * | 2016-04-18 | 2016-07-13 | 长兴盛强电子器材有限公司 | Inductor |
| CN206173355U (en) * | 2016-09-07 | 2017-05-17 | 山东大学 | A electromagnetic environment generator for microbial cultivation test |
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