CN104273320A

CN104273320A - Method for starting fermentation on straw protein feeds at low temperature by composite microorganisms

Info

Publication number: CN104273320A
Application number: CN201310318265.1A
Authority: CN
Inventors: 罗福仲
Original assignee: Individual
Current assignee: Individual
Priority date: 2013-07-14
Filing date: 2013-07-14
Publication date: 2015-01-14

Abstract

The invention discloses a method for starting fermentation on straw protein feeds at low temperature by composite microorganisms. A composite microorganism fermenting agent consists of the components in percentage by mass: (1.5-7.5)x10<6>cfu/g of trichoderma viride, (1.5-7.5)x10<6>cfu/g of aspergillus niger, (1.0-4.0)x10<6>cfu/g of geotrichum candidum, (1.0-4.0)x10<6>cfu/g of candida lambica, (0.45-3.0)x10<6>cfu/g of bacillus, (0.15-1.5)x10<6>cfu/g of pediococcus acidilactici, 1-2 percent of urea, 0.1-0.5 percent of monopotassium phosphate, 0.01-0.1 percent of magnesium chloride, 0.5-1.5 percent of an organic chelating promote yeast agent and 0.1-0.5 percent of humate in a solid culture medium; the solid culture medium comprises the components in percentage by mass: 60-90 percent of straws, 5-20 percent of sweet potato waste and 10-30 percent of chrysanthemum slag. Due to repeated screening on microorganism strains and optimized combination of auxiliaries, the straws can be normally fermented under a condition of low temperature being 0 DEG C, so that the straw fermented protein feeds can be continuously produced all the year round.

Description

The method of complex microorganism cold-starting fermented stalk protein feed

Technical field

The present invention relates to fermentative feedstuff of microbe technical field, specifically a kind of method of complex microorganism cold-starting fermented stalk protein feed.

Background technology

As everyone knows, when environment temperature is lower than 15 DEG C, in especially winter less than 0 DEG C, it is the ubiquitous technical barrier of domestic and international feed fermentation industry that fermentation is difficult to startup, does not still have effective solution so far.Because low temperature cannot start fermentation, cause proceeding normal feed fermentation.The current method that can adopt is, inside solid medium of being nuzzled by hot-water bottle, utilizes the water temperature in hot-water bottle to improve the temperature of solid medium.This method has certain booster action to a small amount of solid medium, can only in the raiser sporadicly raised scattered Emergency use, cannot apply in large-scale production.

On December 15th, 2010, Gazette of Patent for Invention discloses complex microbial inoculum for swine production by fermentation bed being called started quickly at low temperature and preparation method thereof, application number 201010224785.2, publication number CN101914443A.Adopt Trichoderma 15 ~ 20%, bacillus subtilis 15 ~ 20%, Rhodopseudomonas spheroides 15 ~ 20%, inoculating proportion addicted to sour Pediococcus acidilactici 15%, saccharomycete 15%, streptomycete 15%, fermentation bed for pig raising is applied, play the features such as warm speed is fast, deodorization functions is strong, fermentation period is short under there is O DEG C of cryogenic conditions, achieve the continuous circulation zymotechnique of fermentation bed.But this Technical Board is limited to the cold-starting fermentation of fermentation bed for pig raising, has no the report for the fermentation of feed especially stalk protein feed cold-starting.

Summary of the invention

The object of the invention is the method that complex microorganism cold-starting fermented stalk protein feed will be provided, by to the repeated screening of microorganism fungus kind and the optimum organization of auxiliary agent, make stalk under 0 DEG C of cryogenic conditions, normally can start fermentation, realize the continual production throughout the year of stalk fermentation protein feed.

The present invention is achieved in that

Fermentation protein feedstuff is processed into by adding compound microbial culture starter in the solid medium based on stalk, temperature 28 ~ 35 DEG C, pH is the method for the complex microorganism cold-starting fermented stalk protein feed of 5.0 ~ 7.0, and the mass ratio of compound microbial culture starter in solid medium is Trichoderma viride 1.5 ~ 7.5 × 10 ⁶cfu/g, aspergillus niger 1.5 ~ 7.5 × 10 ⁶cfu/g, geotrichum candidum 1.0 ~ 4.0 × 10 ⁶cfu/g, the comparable Candida 1.0 ~ 4.0 × 10 of youth ⁶cfu/g, bacillus 0.45 ~ 3.0 × 10 ⁶cfu/g, Pediococcus acidilactici 0.15 ~ 1.5 × 10 ⁶cfu/g, urea 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.5%, magnesium chloride 0.01 ~ 0.1%, organic chelated leaven promoting agent 0.5 ~ 1.5%, humate 0.1 ~ 0.5%.

The mass ratio of solid medium is: stalk 60 ~ 90%, sweet potato waste 5 ~ 20%, chrysanthemum slag 10 ~ 30%.

Trichoderma viride and bacillus have normally can breed under O DEG C of cryogenic conditions, the characteristic of metabolism, by the temperature of Quick high solid culture medium, be that other microbial growth creates suitable temperature conditions.Meanwhile, Trichoderma viride, bacillus and aspergillus niger have splendid degradation capability to the lignin of stalk high-load, crude fibre, lignin, crude fibre are degraded in a large number and are converted into monose, oligosaccharides homenergic material, meeting other microbial growth demand.Geotrichum candidum and the comparable Candida of youth after obtaining suitable temperature conditions and necessary energy matter, Fast-propagation, and mycoprotein nitrogen-containing compound being synthesized high-quality.Because the pH value in aerobic fermentation process constantly raises, can affect the synthesis of normal fermentation and mycoprotein when reaching more than pH7.0, solid medium then can be maintained the optimum range of pH5.0 ~ 7.0 by the acidic materials such as the lactic acid that Pediococcus acidilactici metabolism produces.Add appropriate urea, potassium dihydrogen phosphate and magnesium chloride in formula to can be complex microorganism especially the geotrichum candidum of synthesising thalli protein and the comparable Candida of youth provide sufficient nutrition assurance.The introducing of organic chelated leaven promoting agent and humate, then significantly can promote breeding, the metabolism of complex microorganism.

The Free water, particularly free moisture of the moisture affecting aerobic fermentation mainly in solid medium.Owing to adding in stalk, the proportion accounting for gross mass 10 ~ 30% is little especially, the king-sized chrysanthemum slag of water absorption is as water absorbing agent and raising agent, solid medium free moisture is made to control 30 ~ 50%, and be in splendid loose ventilative state, while the growth, breeding of promotion aerobic microbiological, the heat that fermentation produces can be distributed timely, make solid medium temperature remain at optimum 28 ~ 35 DEG C of scopes of microorganism, up to the aerobic fermentation of the fresh straw of 70 ~ 85%, there is king-sized using value for moisture content.Because sweet potato waste contains the starch of about 40%, in stalk, add 5 ~ 20% sweet potato wastes, for the normal fermentation of the low stalk of energy level provides reliable energy foundation.

Detailed description of the invention

Formula of the present invention, is provided by following examples.

Batching	Specification	Mass ratio
			Sweet potato vine	Moisture content 60% after dehydration	2500kg
Sweet potato waste	Moisture content≤13%	150kg
			Chrysanthemum slag	Moisture content≤13%	150kg
Trichoderma viride	≥150×10 ⁸cfu/g	0.3Kg
			Aspergillus niger	≥150×10 ⁸cfu/g	0.3kg
Geotrichum candidum	≥20×10 ⁸cfu/g	1kg
			The comparable Candida of youth	≥20×10 ⁸cfu/g	1kg
Bacillus	≥150×10 ⁸cfu/g	0.05kg
			Pediococcus acidilactici	≥150×10 ⁸cfu/g	0.02kg
Urea	Technical grade, N >=46%	15kg
			Potassium dihydrogen phosphate	Technical grade, >=98.0%	5kg
Magnesium chloride	Technical grade, >=98%	0.5kg
			Organic chelated leaven promoting agent	Feed grade	0.5kg
Humate	Feed grade	0.5kg

Winter temperature is-5 ~ 0 DEG C, solid medium pH6.2.Before solid-substrate fermentation, crude protein content is 4%.

Technological process of the present invention, is provided by following examples.

Fresh sweet potato vine → fragmentation → press dewatering → add sweet potato waste, chrysanthemum slag mixing and stirring → add compound microbial culture starter mixing and stirring → pile is high to 100cm aerobic fermentation 3 days → dry → packaging

Ferment 24 hours, the temperature of solid medium can reach 28 ~ 35 DEG C, pH5.0 ~ 7.0 between yeast phase.Ferment 3 days, solid culture primary surface and inside are all covered with mycelia.

Above-mentioned finished product, through inspection, namely becomes the high-quality feed batching of qualified sweet potato vine biofermentation.Primary quality measure is: moisture content≤13%, crude protein content>=18%, crude fiber content≤12%, crude ash content≤8%, effective CFU>=1 × 10 of probio ⁸cfu/g.

Claims

1. the method for complex microorganism cold-starting fermented stalk protein feed, fermentation protein feedstuff is processed into by adding compound microbial culture starter in the solid medium based on stalk, temperature is 28 ~ 35 DEG C, pH is 5.0 ~ 7.0, it is characterized in that the mass ratio of compound microbial culture starter in solid medium is Trichoderma viride 1.5 ~ 7.5 × 10 ⁶cfu/g, aspergillus niger 1.5 ~ 7.5 × 10 ⁶cfu/g, geotrichum candidum 1.0 ~ 4.0 × 10 ⁶cfu/g, the comparable Candida 1.0 ~ 4.0 × 10 of youth ⁶cfu/g, bacillus 0.45 ~ 3.0 × 10 ⁶cfu/g, Pediococcus acidilactici 0.15 ~ 1.5 × 10 ⁶cfu/g, urea 1 ~ 2%, potassium dihydrogen phosphate 0.1 ~ 0.5%, magnesium chloride 0.01 ~ 0.1%, organic chelated leaven promoting agent 0.5 ~ 1.5%, humate 0.1 ~ 0.5%.

2. the method for complex microorganism cold-starting fermented stalk protein feed according to claim 1, is characterized in that the mass ratio of solid medium is: stalk 60 ~ 90%, sweet potato waste 5 ~ 20%, chrysanthemum slag 10 ~ 30%.