CN104264658B - A kind of method that actinomyces are fixed the sand - Google Patents
A kind of method that actinomyces are fixed the sand Download PDFInfo
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- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
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- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/12—Consolidating by placing solidifying or pore-filling substances in the soil
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Abstract
The present invention relates to the new method that a kind of actinomyces are fixed the sand, does the method choose actinomyces naeslundii Actinomyces? naeslundii, then the nutritious soup culture medium enrichment be placed in by actinomyces containing urea and calcium ion obtains having the actinomyces bacterium liquid of induced curing effect, again enrichment is obtained solidification actinomyces bacterium liquid and be evenly sprayed on sandy soil top layer several times, experiment is stopped after cultivating a couple of days under the sandy soil being sprayed with bacterium liquid are placed in natural drying environment, actinomyces by cementing for the grains of sand on sandy soil top layer fixing, make the top layer grains of sand to move by solidification.The method is easy and simple to handle, be easy to control, and cost is low, can be widely used in arid biogeographic zone and move the fixing of half migrating dune.
Description
Technical field
The present invention relates to a kind of method that actinomyces are fixed the sand, the actinomyces especially with solidification fix the method on removable sand dune, belong to bioenvironment engineering field.
Background technology
Desertification is a worldwide ecological environment problem.China is one of country that desert area is larger, distributed more widely, desertification hazard is serious in the world.According to the desertification findings of the survey in the 1994-1996 whole nation, national desert and the desertification gross area account for 17.85% of territory total area.For a long time due to the aggravation of drought and mankind's activity, the unreasonable use in soil, to the deficiency that desertification treatment drops into, causes serious land deterioration.According to investigations, from the fifties to the seventies, desertification land increases 1560km every year on average
2; The mid-1970s is to the mid-80, and desertification land increases 2100km every year on average
2, from the mid-80 to the nineties, desertification land increases 2460km every year on average
2.At present still in the trend continuing to expand, in the north ten thousand on dust storm line, the large dust storm day of annual more than 8 grades is 30 ~ 100 days, also often occur strong chromatic number, China three Norths shelter-forest area, comprises portion of Western China Beijing University, North of North China and West of The Northeast China, there are a large amount of farmland and grassland every year by hazards of sand storms,, thousands of reservoirs and large quantities of feed ditch are attacked by dust storm, and more than 800 kilometer of railway and thousands of miles highway are often subject to the invasion and attack of dust storm and pressure is buried.
Desertification is constantly expanded, and sandstorm is more and more frequent, not only causes very big destruction to production and construction, and brings heavy losses to people's lives and properties.Desertification destroyed land resource, makes to utilize soil to reduce, Quality Down, causes the agriculture-stock production underproduction even to have no harvest.Destroy the production basis such as traffic, water conservancy facility, restriction economic development.Desertification exacerbates peasants and herdsmen's poverty degree, affects social stability and national unity, and desertification makes environment for human survival worsen, and arable land, grassland, forest land etc. can utilize land resources Quality Down or productivity to lose, and destroy and produce and living facilities; Desertification makes living beings quantitative change bad, and species abundance reduces, and constitutes a serious threat to bio-diversity.Therefore, desertification is the key factor of arid, semiarid zone generation poverty.
Current China actively renovates desertification, and the gratifying situation that people enters sand and moves back has been there is in some areas, but all in all or sand enters people and moves back, Desertification Soil people is in continuous expansion, arable land, grassland are reduced, environment worsens, and serious constrains the national economic development, and desertification situation is very severe.At present, fix the sand that common technology mainly contains that engineering (or physical/mechanical) is fixed the sand, sand consolidation with biologic, chemical sand-fixing and comprehensively fix the sand.
Engineering sand control mainly takes mechanical path, by the resistance to dust storm, defeated, lead, solid engineering reaches and alleviates eolian action, prevents the object of hazards of sand storms.Two large classes are comprised at present: (1) lays straw checkerboard barrier, sets up three-dimensional fence, arranges the dune work technology of various material web with the engineering sand control technology had; (2) water diversion for sand removal, technology of reclaiming land by sand control.It is the water source utilizing river, sea (lake), reservoir, and artesian diversion or machinery draw water, and draws sand dune, both eliminated sand damage, and expanded again the way in arable land with waterpower punching.This is the invention of the husky district masses of North Shaanxi Province of China, and has the place at water source to apply at other.
Sand consolidation with biologic is by fencing, builds the means such as plant, reaches control desert, stablizes oasis, improve a kind of technical measures of husky district environmental quality and productive potentialities.Mainly contain following several sand fixing technology: the protection of (1) natural vegetation and Appropriate application technology (i.e. fencing technology), this is the main approach protecting natural vegetation and bio-diversity thereof at present; (2) plant seeds by airplane sand control technology, is utilize aircraft to carry out the mechanization method of forestation sowed in husky district or arid area, is suitable for the improvement in underpopulated remote husky district, and plant seeds by airplane sand control area is large, and effect of checking winds and fixing drifting sand is good; (3) build forests for windbreak and sand-fixation technology, forest belt China the prevents and control sandy storm huge engineering corroded that what China built in three northern areas of China check winds and fix drifting sand, existing oneself begins to take shape, and plays a good role to preventing dust storm erosion; (4) Planting Grass and Pasture improvement technology; (5) agricultural protection forest network technology; (6) fruticeta's technology is built; The technology of defending and controlling sand of (7) five band one, sets up envelope sand-base cultivation straw cord, resistance sand ribbon, forward position, grass barrier vegetational zone, irrigates afforestation band, fixes the sand fire belt, the way adopting plant sand block and mechanical sand barrier to combine, builds up firm system of checking winds and fixing drifting sand, is mainly used in railway, both sides of highway.As the blue railway of bag through Tengger desert, be with at In Shapotou of Ningkia one and adopt this technology to establish at the sea of sand green screen that reaches more than 30 kilometer, pinned drift sand, ensure that the unimpeded of the blue railway of bag.
Chemical sand-fixing is under wind sand environment, utilize chemical material and technique, the sand dune of commute generation sand damage or sandy earth's surface are built one deck and can be prevented and treated wind-force and blow the bonding course raised and have and keep moisture and improvement sand ground character, control to reach and improve sand damage environment, improving the technical measures of sand ground productivity.Chemical sand-fixing technology, contain sand ground concretion technology and the large content of water conservation getting fat two, it and vegetative sand control combine and greatly can improve the survival rate of plant.External chemical sand-fixing research starts from the thirties in 20th century, domesticly start from the sixties in 20th century, existing 46 countries develop 100 number of chemical preparations in the world, for the reclamation of drift sand, mainly contain following four large classes: the sand fixation materials such as cement paste, water glass class, oil product class and high molecular polymer super absorbent resin lipid.
Various single Sand-fixing Measures has its feature and limitation, though engineering is fixed the sand effective fast, cost high and with biotic environment poor compatibility; Though sand consolidation with biologic has good ecological environmental protection effect, because of desert or sand ground harsh climate hydrologic condition, cannot survive after usually making plant plant or to plant; Though chemical sand-fixing is with low cost, quick and convenient, good biocompatibility of constructing, the cycle of usually fixing the sand is shorter; With regard to large area is administered, modern sediment control structure requires high efficiency low cost, quick and convenient.
The scientific and technical personnel of China are from long-term production practices, and after finding that moving dunes are fixing, it is that sand ground forms complete skinning cortex that ecological environmental condition the most significantly changes.The effect of biological breadcrust in desertification treatment is mainly reflected in containment soil desertification and provides good basic environment for the ecosystem restoration of desertificated area.The underground mycelia of bacterium in biological breadcrust, fungi, lichens and bryophyte and rhizoid can bond soil particle, and form constitutionally stable organic and inorganic composite bed, thus change single, homogeneous, the loose reset condition in desertification soil surface, make soil surface be tending towards immobilization.Biological breadcrust efficiently reduces wind and water to the erosion on desertification earth's surface, thus has contained desertification process to a certain extent.Therefore, in desertification treatment work, the appearance of biological breadcrust and development indicate the important stage that flowing sand ground transforms to fixing and semi-fixed sandy land, can be used as ecological environment health assessment indicators.As far back as 1900, ecologist just started the multiple effect (Johansen, 1993) paying attention to biological breadcrust, and this effect mainly comprises the ability of fixing soil; Affect Nutrient Cycling and vascular plant community etc.In recent years, it is how to adapt to arid and semiarid land ecosystem that increasing researcher is inquiring into biological breadcrust, study desert biological breadcrust artificial infection sand fixing technology on this basis, this technology be utilize desert algae plant a colony sand fixation, artificial infection desert algae in traditional sand-fixing vegetation band, promote the formation of desert algae biological breadcrust, and set up the comprehensive sand control technology that the stable complex ecosystem of dissimilar algae, draft, shrub, arbor is target with suiting measures to local conditions, open up the new way of desertification treatment.Bacterium is the microorganism type extensively existed in the environment of arid desert, to play in the forming process of biological breadcrust and important effect, many research finds that some Special Bacteria can pass through solidification curing soil, this thinking new for desertification treatment provides under proper condition.
Summary of the invention
The object of the invention is to, the new method that a kind of actinomyces are fixed the sand is provided, the actinomyces naeslundii Actinomycesnaeslundii that the method is chosen, then the nutritious soup culture medium enrichment be placed in by actinomyces containing urea and calcium ion obtains having the actinomyces bacterium liquid of induced curing effect, again enrichment is obtained solidification actinomyces bacterium liquid and be evenly sprayed on sandy soil surface several times, experiment is stopped after cultivating a couple of days under the sandy soil being sprayed with bacterium liquid are placed in natural drying environment, actinomyces are fixed cementing for the grains of sand on sandy soil surface by solidification, the top layer grains of sand can not be moved.The method is easy and simple to handle, be easy to control, and cost is low, can be widely used in arid biogeographic zone and move the fixing of half migrating dune.
The new method that a kind of actinomyces of the present invention are fixed the sand, follows these steps to carry out:
A, actinomyces source and cultivation: choose actinomyces naeslundii Actinomycesnaeslundii, the conical flask containing 50mL nutritious soup culture medium is forwarded to after bacterial strain being placed in nutrient broth medium recovery, pH is adjusted to 4-11, then conical flask is placed in that rotating speed is 130 revs/min, temperature is cultivate 48 hours under the condition of 30 DEG C, obtain actinomyces bacterium liquid;
B, induced curing effect actinomyces domestication enrichment: the actinomyces bacterium liquid obtained by step a is placed in urea that mass content is 1-20%, the nutritious soup culture medium of 0.01-0.5 mol/L calcium ion mixes, mixed liquor is placed in rotating speed is 130 revs/min, temperature is cultivate enrichment in 5-7 days under the condition of 30 DEG C to obtain solidification bacterium liquid, bacterium liquid pH is 4-11;
C, induced curing effect actinomyces are fixed the sand: the actinomyces bacterium liquid obtaining step b enrichment to have solidification is sprayed at sandy soil top layer in a 10-100 milliliter/100 gram husky ratio, and wherein in bacterium liquid, strain density is 10
4-10
8cFU/ milliliter, after cultivating 7-15 days under sandy soil after sprinkling bacterium liquid are placed in natural drying environment, again spray after identical bacterium liquid is placed in natural environment natural air drying for 2-5 time and stop experiment, actinomyces are able to fixing sandy soil top layer by solidification, stop sandy soil to move under wind-force effect.
Nutritious soup culture medium described in step b is nutrient broth and rice washing water.
After actinomyces solidification described in step c, sandy soil top layer can form the hard shell of one deck, can resistance to wind speed be 10-70 meter per second.
Actinomyces described in step c are fixed the sand by the effect of hydrolyze urea induction mineral precipitation.
The new method that a kind of actinomyces of the present invention are fixed the sand, actinomyces information in the method: Classification And Nomenclature: actinomyces naeslundii, latin name: Actinomycesnaeslundii, depositary institution and preserving number: American Type Culture Collection center; Preserving number: ATCC12104, Chinese agent address: No. 11 Beijing, Oriental East Road, Chaoyang District, Beijing City Central Plains company, time buying: on September 8th, 2013.
The new method that a kind of actinomyces of the present invention are fixed the sand, the method is the actinomyces bacterium liquid that the nutritious soup culture medium enrichment actinomyces with induced curing effect being placed in urea that mass content is 1-20% and 0.01-0.5 mol/L calcium ion obtains having solidification; The actinomyces bacterium liquid 10-100 milliliter again enrichment being obtained solidification is evenly sprayed at 100-200 gram of sandy soil surface, after cultivating 7-15 days under sandy soil after sprinkling bacterium liquid are placed in daily dry environment, stop experiment after being placed in natural environment natural air drying after again spraying identical bacterium liquid for 2-5 time, actinomyces are able to fixing sandy soil top layer by solidification.
The new method that a kind of actinomyces of the present invention are fixed the sand, actinomyces source and cultivation in the method: choose actinomyces naeslundii (Actinomycesnaeslundii), bacterial strain is from from American Type Culture Collection center (ATCC12104), the conical flask containing 50mL nutritious soup culture medium is forwarded to after bacterial strain being placed in nutrient broth medium (NB) recovery, pH is adjusted to 4-11, then conical flask is placed in that rotating speed is 130 revs/min, temperature is cultivate 48 hours under the condition of 30 DEG C, obtain actinomyces bacterium liquid;
There is induced curing effect actinomyces domestication enrichment: actinomyces enrichment obtained are placed in the nutritious soup culture medium of urea that mass content is 1-20%, 0.01-0.5 mol/L calcium ion, mixed liquor is placed in rotating speed is 130 revs/min, temperature is cultivate enrichment in 5-7 days under the condition of 30 DEG C to obtain solidification bacterium liquid, bacterium liquid pH is 4-11;
There are induced curing effect actinomyces fix the sand: the actinomyces bacterium liquid obtaining enrichment to have solidification is sprayed at sandy soil top layer according to a 10-100 milliliter/100 gram husky ratio, and wherein in bacterium liquid, strain density is 10
4-10
8cFU/ milliliter, after cultivating 7-15 days under sandy soil after sprinkling bacterium liquid are placed in natural drying environment, again spray after identical bacterium liquid is placed in natural environment natural air drying for 2-5 time and stop experiment, actinomyces are able to fixing sandy soil top layer by solidification, stop sandy soil to move under wind-force effect.
Actinomyces culture medium selected in method of the present invention is nutrient broth and rice washing water; Selected sandy soil are Bei Shawo top layer, ancient capital Xi'an Fukang, Xinjiang Uygur Autonomous Regions sandy soil, and after actinomyces solidification, sandy soil top layer can form the hard shell of one deck, can resistance to wind speed be 10-70 meter per second; Selected actinomyces are for can hydrolyze urea and have induced curing effect.
The method of the invention major advantage: 1. the method adopts the hydrolysis of urea actinomyces fixed dune surface with solidification, its mechanism is that sandy soil granular cementation is formed stable crust by actinomyces induction mineral precipitation, and its sand-fixing effect is stablized and environmental protection; 2. the method moves drought environment, the stationary applications of half migrating dune is extensive, simple to operate, cost-saving.
Accompanying drawing explanation
Fig. 1 is the original sandy soil figure of the present invention;
Fig. 2 is the sandy soil figure after bacterial immobilisation effect of the present invention.
Detailed description of the invention
The invention is not restricted to following examples:
The new method that a kind of actinomyces of the present invention are fixed the sand, follows these steps to carry out:
A, actinomyces source and cultivation: choose actinomyces naeslundii Actinomycesnaeslundii, the conical flask containing 50mL nutritious soup culture medium is forwarded to after bacterial strain being placed in nutrient broth medium recovery, pH is adjusted to 4-11, then conical flask is placed in that rotating speed is 130 revs/min, temperature is cultivate 48 hours under the condition of 30 DEG C, obtain actinomyces bacterium liquid;
B, induced curing effect actinomyces domestication enrichment: the actinomyces obtained by step a are placed in urea that mass content is 1-20%, the nutritious soup of 0.01-0.5 mol/L calcium ion is that nutrient broth and rice washing water culture medium mix, mixed liquor is placed in rotating speed is 130 revs/min, temperature is cultivate enrichment in 5-7 days under the condition of 30 DEG C to obtain solidification bacterium liquid, bacterium liquid pH is 4-11;
C, induced curing effect actinomyces are fixed the sand: the actinomyces bacterium liquid obtaining step b enrichment to have solidification is sprayed at husky nest sandy soil top layer, north, ancient capital Xi'an Fukang, Xinjiang Uygur Autonomous Regions according to a 10-100 milliliter/100 gram husky ratio, and wherein in bacterium liquid, strain density is 10
4-10
8cFU/ milliliter, after cultivating 7-15 days under sandy soil after sprinkling bacterium liquid are placed in natural drying environment, again spray after identical bacterium liquid is placed in natural environment natural air drying for 2-5 time and stop experiment, actinomyces are able to fixing sandy soil top layer by hydrolyze urea induction mineral precipitation solidification, form the shell that one deck is hard, 10-70 meter per second can be reached by resistance to wind speed, stop sandy soil to move under wind-force effect; Specific embodiment adopts form to illustrate:
Here is the specific embodiment of the method that the actinomyces of induced curing effect are fixed the sand:
The product reaching the object of the invention can be produced by the various embodiments described above batching.By the method for the invention, show through nest experiment husky in north, ancient capital Xi'an Fukang, Xinjiang Uygur Autonomous Regions: all can reach sand-fixing effect.
Claims (4)
1. an actinomyces method of fixing the sand, is characterized in that following these steps to carry out:
A, actinomyces source and cultivation: choose actinomyces naeslundii Actinomycesnaeslundii, the conical flask containing 50mL nutritious soup culture medium is forwarded to after bacterial strain being placed in nutrient broth medium recovery, pH is adjusted to 4-11, then conical flask is placed in that rotating speed is 130 revs/min, temperature is cultivate 48 hours under the condition of 30 DEG C, obtain actinomyces bacterium liquid;
B, induced curing effect actinomyces domestication enrichment: the actinomyces bacterium liquid obtained by step a is placed in urea that mass content is 1-20%, the nutritious soup culture medium of 0.01-0.5 mol/L calcium ion mixes, mixed liquor is placed in that rotating speed is 130 revs/min, temperature is cultivate enrichment in 5-7 days under the condition of 30 DEG C to obtain the actinomycetic bacterium liquid of induced curing effect, bacterium liquid pH is 4-11 again;
C, induced curing effect actinomyces are fixed the sand: step b enrichment is obtained induced curing effect actinomycetic bacterium liquid and be sprayed at sandy soil top layer in a 10-100 milliliter/100 gram husky ratio, wherein in bacterium liquid, strain density is 10
4-10
8cFU/ milliliter, after cultivating 7-15 days under sandy soil after sprinkling bacterium liquid are placed in natural drying environment, again spray after identical bacterium liquid is placed in natural environment natural air drying for 2-5 time and stop experiment, induced curing effect actinomyces are able to fixing sandy soil top layer by solidification, stop sandy soil to move under wind-force effect.
2. a kind of actinomyces according to claim 1 method of fixing the sand, is characterized in that the nutritious soup culture medium described in step b is nutrient broth and rice washing water.
3. a kind of actinomyces according to claim 1 method of fixing the sand, after it is characterized in that the actinomyces solidification described in step c, sandy soil top layer can form the hard shell of one deck, can resistance to wind speed be 10-70 meter per second.
4. a kind of actinomyces according to claim 1 method of fixing the sand, is characterized in that the actinomyces described in step c are fixed the sand by the effect of hydrolyze urea induction mineral precipitation.
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| CN106759248B (en) * | 2016-12-29 | 2018-09-21 | 河海大学 | Barren green desert earth's surface windproof structure |
| CN106818362B (en) * | 2016-12-29 | 2018-05-11 | 河海大学 | Barren green desert pattern of farming and in desert planting plants method |
| CN106856700B (en) * | 2016-12-29 | 2018-05-11 | 河海大学 | Desert planting structure and in desert planting plants method |
| CN106979029B (en) * | 2017-05-11 | 2019-03-19 | 中国科学院新疆生态与地理研究所 | A method of prevent open coal mine dust from dissipating using microorganism and saline groundwater |
| CN107983745A (en) * | 2017-11-24 | 2018-05-04 | 国网新疆电力有限公司电力科学研究院 | Application of the actinomyces naeslundii in cupro-nickel tailing solidifying |
| CN110576023A (en) * | 2019-07-26 | 2019-12-17 | 国网新疆电力有限公司电力科学研究院 | Application of Actinomyces naeslundii in solidification of copper-molybdenum tailings |
| CN112077122A (en) * | 2020-09-07 | 2020-12-15 | 国网新疆电力有限公司电力科学研究院 | Method for solidifying iron tailings by inducing solidification effect bacteria |
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| CN1769421A (en) * | 2005-09-01 | 2006-05-10 | 中国科学院新疆生态与地理研究所 | Sand-fixing water-keeping sprout-protecting bacteria preparation of low nutrient tolerance |
| CN101368384A (en) * | 2008-09-19 | 2009-02-18 | 东南大学 | Method for curing soil by using carbonate mineralized bacterium |
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| JPS55135191A (en) * | 1979-04-09 | 1980-10-21 | Nitto Chem Ind Co Ltd | Stabilization of soil |
| CN1769421A (en) * | 2005-09-01 | 2006-05-10 | 中国科学院新疆生态与地理研究所 | Sand-fixing water-keeping sprout-protecting bacteria preparation of low nutrient tolerance |
| CN101368384A (en) * | 2008-09-19 | 2009-02-18 | 东南大学 | Method for curing soil by using carbonate mineralized bacterium |
| CN102732255A (en) * | 2012-06-25 | 2012-10-17 | 广州中茂园林建设工程有限公司 | Soil improved composition and method for improving soil |
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