CN107983745A - Application of the actinomyces naeslundii in cupro-nickel tailing solidifying - Google Patents
Application of the actinomyces naeslundii in cupro-nickel tailing solidifying Download PDFInfo
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- CN107983745A CN107983745A CN201711188973.2A CN201711188973A CN107983745A CN 107983745 A CN107983745 A CN 107983745A CN 201711188973 A CN201711188973 A CN 201711188973A CN 107983745 A CN107983745 A CN 107983745A
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- actinomyces naeslundii
- nickel tailing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/20—Waste processing or separation
Abstract
The present invention relates to cupro-nickel tailing solidifying art field, is a kind of application of actinomyces naeslundii in cupro-nickel tailing solidifying.Application of the first public actinomyces naeslundii of the present invention in cupro-nickel tailing solidifying, acted on by the biological curing of actinomyces naeslundii, cure cupro-nickel tailings particles, make its curing blocking, at the same time by copper ion in cupro-nickel tailing, the heavy metal ion such as nickel ion are also secured in its block, after curing process, since cupro-nickel tailing is in bulk, therefore, it is resistant to windability and compressive resistance greatly improves, make it when meeting with strong wind weather, it will not be scraped scattered, also it is avoided that causing large-scale environmental pollution, at the same time, substantially reduce the heavy metal ion in cupro-nickel tailing and radioactive substance flows into river nearby and the amount to permeate the ground, it is significantly reduced to pollute caused by river and underground water and radiolesion, in addition, occupancy and destruction of the cupro-nickel tailing to land resource can be significantly reduced.
Description
Technical field
The present invention relates to cupro-nickel tailing solidifying art field, is a kind of application of actinomyces naeslundii in cupro-nickel tailing solidifying.
Background technology
Mineral resources just become the important substance resource of people's existence and social development from it is found utilization
One of.China is the big producer of non-ferrous metal, after reform and opening-up, although economically there is rapid development in China, is achieved
Remarkable achievement.Many environmental problems for being difficult to administer can be also result in, wherein with tail due to extensive development model
Environmental problem caused by ore deposit is especially prominent.Explored mineral resources allowable exploitation is huge at present in China, but they are mostly
It is low-grade and 80% or so is all mineral resources existing in the form of intergrowth mineral stone.The development and utilization of mineral resources is
It is basic with building national that China develops the economy, but it belongs to Depletion resource again, using non-renewable and implicity as
Its feature, is a kind of very important natural resources.The various aspects of people's life are benefited in the utilization of mineral resources, right
Social progress and development play a crucial role, and are one of most important social production data.Since the past is to limited
Mineral resources carry out the exploitation of predation formula, and the value of main mineral products is too paid close attention in recovery process and have ignored its association
The economic value of component so that nowadays tailing has become that China or even world's volume of cargo in storage are most, comprehensive utilization ratio is minimum consolidates
Body waste.Shown according to the statistics of 2011, the volume of cargo in storage of China's tailing is more than 10,000,000,000 tons at present, but also with annual
1200000000 tons or so of the speed of response increases, since dosage is huge and utilization rate is low so that the year quantum of output of the current tailing in China accounts for
More than the 50% of whole world tailing quantum of output.
Tailings glass or Disposal Measures can not endangered caused by surrounding enviroment at that time is mainly manifested in the following aspects：
First, substantial amounts of tailing is disposed in a manner of stockpiling at present, this can take and destroy the substantial amounts of soil money in China
Source, with the increase of tailing heap storage, can increasingly sharpen the contradiction that China has a large population and a few land, constraint China agricultural, the development of forestry.
Statistics has shown that more than 40 years since reform and opening-up, China was because of the cultivated area of the reason reduction such as tailing and soil erosion
Reach 4 × 107Mu or so, it is annual caused by economic loss more than 10,000,000,000 RMB；Second, all contain in most tailing
There is the poisonous and hazardous chemical substance such as the heavy metal ion such as Pb, Cu, Cr and As, and it is certain containing in ore dressing or smelting in tailing
The various chemical agents that can cause environmental pollution added during refining, these poisonous and harmful substances all can more or less with
Snow melts, drench with rain or tailing water is flowed into and nearby river and permeated the ground, and causes the pollution of river and underground water, and local contaminated
Ecological environment needs upper a century to recover, and some is even temporary transient to be recovered；Third, the tailing of natural air drying, particle diameter very little and
Light weight, can be blown to surrounding area when running into strong wind, in turn result in larger range of environmental pollution.Such as twentieth century 90
Age, positioned at the nickel minerals mining area in China Jinchuan, just as to cause boundless mad sand to break out sand dust sudden and violent for the nickel tailing that strong wind is swept, it is right
Local ecological environment causes serious pollution；Four be due to make in some primary ores containing radioactive substances such as uranium, radium
Obtaining the tailing that enterprise discharges in smelting or ore dressing process also has certain radioactivity, this to contain the radioactive materials such as uranium, radium
The tailing of matter can cause irreversible radioactive pollution after largely stacking to the environment on periphery and its organism, when people touches this
It can also be damaged during a little radioactive substances.
Therefore, because caused by above-mentioned tailing it is numerous it is dangerous with harm and social development and improve to mineral resources according to
Rely so that one of China or even the world great environmental problem urgently to be resolved hurrily is had become to dealing carefully with for tailing and disposal.
The content of the invention
The present invention provides a kind of application of actinomyces naeslundii in cupro-nickel tailing solidifying, overcome the above-mentioned prior art it
Deficiency, it can effectively solve the problems, such as that existing tailings glass has serious pollution environment.
The technical scheme is that realized by following measures：A kind of actinomyces naeslundii is in cupro-nickel tailing solidifying
Application.
Here is the further optimization and/or improvements to foregoing invention technical solution：
Application of the above-mentioned actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by actinomyces naeslundiiActinomyces naeslundiiThe mass percent for being placed in urea is 2% to 10%, the concentration of calcium ion is 0.2 mol/L
The bacterium solution with induced curing effect is obtained after being cultivated 1 day to 3 days into the nutrient broth nutrient solution of 1.5 mol/Ls, wherein,
Nutrient broth nutrient solution and the pH of bacterium solution are 5 to 10, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL to 108
CFU/mL；Second step, adds 30mL to the ratio of 120mL bacterium solutions in every 100 grams of cupro-nickel tailings, bacterium solution is sprayed at cupro-nickel tailing
In, the cupro-nickel tailing after sprinkling bacterium solution is placed in spontaneously dry and is cultivated 10 days to 15 days under environment, then after natural air drying, consolidate
Change cupro-nickel tailing.
Above-mentioned bacterium solution obtains as follows：Actinomyces naeslundii bacterial strain is placed in nutrient broth medium after recovering, turned
Be connected to pH be 5 to 10 nutritious soup culture medium in, temperature be 30 DEG C under conditions of culture 48 it is small when after obtain bacterium solution.
Application of the first public actinomyces naeslundii of the present invention in cupro-nickel tailing solidifying, is consolidated by the biology of actinomyces naeslundii
Change act on, cure cupro-nickel tailings particles, make its curing blocking, at the same by the heavy metal such as copper ion, nickel ion in cupro-nickel tailing from
Son is also secured in its block, after curing process, since cupro-nickel tailing is in bulk, and, it is resistant to windability and pressure-resistant
Intensity greatly improves, and makes it when meeting with strong wind weather, will not be scraped it is scattered, also it is avoided that causing large-scale environmental pollution, together
When, substantially reduce the heavy metal ion in cupro-nickel tailing and radioactive substance flows into river nearby and the amount to permeate the ground, i.e., it is aobvious
Writing reduces it pollutes and radiolesion caused by river and underground water, furthermore it is possible to significantly reduce cupro-nickel tailing to soil
The occupancy of resource and destruction, based on the above, application of the actinomyces naeslundii in cupro-nickel tailing solidifying, have ecological protection meaning
Justice.
Brief description of the drawings
State diagram of the attached drawing 1 for cupro-nickel tailing without curing process.
The state diagram for the curing cupro-nickel tailing that attached drawing 2 is obtained for cupro-nickel tailing using present invention processing.
Embodiment
The present invention from following embodiments limitation, can technique according to the invention scheme and actual conditions determine specifically
Embodiment.Various chemical reagent and chemical article are previously mentioned in the present invention unless otherwise specified, are public in the prior art
Know common chemical reagent and chemical article；Percentage in the present invention is mass percent as not having specified otherwise；This hair
It is the aqueous solution that solvent is water, for example, hydrochloric acid solution is aqueous hydrochloric acid solution if the solution in bright is without specified otherwise；This
Room temperature, room temperature in invention refer generally to 15 DEG C to 25 DEG C of temperature, are commonly defined as 25 DEG C.
With reference to embodiment, the invention will be further described：
Embodiment 1：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, Nei Shi is put
Line bacteriumActinomyces naeslundiiThe mass percent for being placed in urea is 2% to 10%, the concentration of calcium ion is 0.2 to rub
You/rise in the nutrient broth nutrient solutions of 1.5 mol/Ls cultivate 1 day to 3 days after obtain the bacterium solution with induced curing effect,
Wherein, nutrient broth nutrient solution and the pH of bacterium solution are 5 to 10, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL is extremely
108 CFU/mL；Second step, adds 30mL to the ratio of 120mL bacterium solutions in every 100 grams of cupro-nickel tailings, bacterium solution is sprayed at cupro-nickel
In tailing, the cupro-nickel tailing after sprinkling bacterium solution is placed in spontaneously dry and is cultivated 10 days to 15 days under environment, then after natural air drying, is obtained
To curing cupro-nickel tailing.
Cupro-nickel tailing makes carbon dioxide that urea fast hydrolyzing generates and ammonia be discharged into water by the effect of actinomyces naeslundii
In solution, carbanion and ammonium ion are generated, calcium ion plasma and carbanion generation carbonic acid in cupro-nickel tailing
On the one hand calcium precipitate, precipitation of calcium carbonate can bond cupro-nickel tailings particles, achieve the purpose that to cure tailing, on the other hand due to interior
Family name actinomyces are negatively charged, can adsorb the metal ion of positively charged, and the precipitation of calcium carbonate of generation can also consolidate while bacterium is wrapped up
Determine heavy metal ion, achieve the purpose that curing heavy metal.
In the present embodiment, cupro-nickel tailings particles are cured by the biological curing effect of actinomyces naeslundii, make its curing blocking,
The heavy metal ion such as copper ion, nickel ion in cupro-nickel tailing is also secured in its block at the same time, after curing process, due to copper
Nickel tailing is in bulk, and therefore, it is resistant to windability and compressive resistance greatly improves, and makes it when meeting with strong wind weather, will not
It is scattered by scraping, also it is avoided that causing large-scale environmental pollution, meanwhile, substantially reduce the heavy metal ion in cupro-nickel tailing and radiation
Property material flow into nearby river and the amount that permeates the ground, that is, significantly reduce it and polluted caused by river and underground water and radioactivity
Infringement, furthermore it is possible to occupancy and destruction of the cupro-nickel tailing to land resource be significantly reduced, it follows that the actinomyces naeslundii exists
Application in cupro-nickel tailing solidifying, has ecological protection meaning.
The cupro-nickel tailing of Altay, Xinjiang area Fuyun County Ka Latongke copper nickels factory is handled using actinomyces naeslundii
Afterwards, the top layer of cupro-nickel tailing forms one layer of hard shell, and obtained cupro-nickel tailing is resistant to wind speed and reaches 30 metre per second (m/s)s to 70 meters
It is per second, while the heavy metal ion in cupro-nickel tailing can also be fixed, 1.24 μ g/ have been reached to the fixed amount of nickel in cupro-nickel tailing
G, fixed rate have also reached 15%, fixed amount is in 0.13 μ g/g or so 21.4% to the fixed rate of copper in addition.
The cupro-nickel tailing of Altay, Xinjiang area Fuyun County Ka Latongke copper nickels factory is for example attached without the state of curing process
Shown in Fig. 1.In attached drawing 1, cupro-nickel tailing is in loose sand shape.
Cupro-nickel tailing using the method described in the present embodiment to Altay, Xinjiang area Fuyun County Ka Latongke copper nickels factory
The curing cupro-nickel tailing obtained after being handled is as shown in Figure 2.
In attached drawing 2, cure cupro-nickel tailing in bulk.
The cupro-nickel tailing of Altay, Xinjiang area Fuyun County Ka Latongke copper nickels factory is using described in embodiment 3 to embodiment 11
Method curing process after, tolerance wind-force, compressive resistance, the fixed rate of the fixed rate of nickel ion and copper ion are as shown in table 1.
In the present invention, the bacterium information that uses：Classification And Nomenclature：Actinomyces naeslundii；Latin name：Actinomyces naeslundii；Depositary institution：American Type Culture Collection center；Preserving number：ATCC 12104；Chinese agent address：North
Jing Shi Chaoyang Districts east East Road No. 11 Beijing Central Plains company；Time buying：On September 8th, 2013.
Embodiment 2：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 2% or the concentration of 10%, calcium ion is 0.2
Cultivate 1 day in the nutrient broth nutrient solution of mol/L or 1.5 mol/Ls or obtain the bacterium with induced curing effect after 3 days
Liquid, wherein, the pH of nutrient broth nutrient solution and bacterium solution is 5 or 10, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL
Or 108 CFU/mL；Second step, the ratio of 30mL or 120mL bacterium solutions is added in every 100 grams of cupro-nickel tailings, bacterium solution is sprayed at copper
In nickel tailing, the cupro-nickel tailing after sprinkling bacterium solution is placed in spontaneously dry and is cultivated 10 days or 15 days under environment, then after natural air drying,
Obtain curing cupro-nickel tailing.
Embodiment 3：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 2%, the concentration of calcium ion be 0.2 mole/
Rise nutrient broth nutrient solution in cultivate 3 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution with
The pH of bacterium solution is 5, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 30mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 15 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 4：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 6%, the concentration of calcium ion be 0.2 mole/
Rise nutrient broth nutrient solution in cultivate 3 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution with
The pH of bacterium solution is 5, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 50mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 15 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 5：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 10%, the concentration of calcium ion is 0.2 to rub
You/liter nutrient broth nutrient solution in cultivate 3 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth culture
Liquid and the pH of bacterium solution are 5, and in bacterium solution, the density of actinomyces naeslundii is 104 CFU/mL；Second step, by every 100 grams of cupro-nickel tailings
The ratio of 120mL bacterium solutions is added, bacterium solution is sprayed in cupro-nickel tailing, the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying
Cultivated 15 days under environment, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 6：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 10%, the concentration of calcium ion be 1 mole/
Rise nutrient broth nutrient solution in cultivate 1 day after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution with
The pH of bacterium solution is 8, and in bacterium solution, the density of actinomyces naeslundii is 108 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 30mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 10 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 7：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 2%, the concentration of calcium ion is 1 mol/L
Nutrient broth nutrient solution in cultivate 1 day after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution and bacterium
The pH of liquid is 8, and in bacterium solution, the density of actinomyces naeslundii is 108 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 50mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 10 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 8：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 6%, the concentration of calcium ion is 1 mol/L
Nutrient broth nutrient solution in cultivate 1 day after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution and bacterium
The pH of liquid is 8, and in bacterium solution, the density of actinomyces naeslundii is 108 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 120mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 10 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 9：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, by
Family name actinomycesActinomyces naeslundiiThe mass percent for being placed in urea is 6%, the concentration of calcium ion be 1.5 moles/
Rise nutrient broth nutrient solution in cultivate 2 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth nutrient solution with
The pH of bacterium solution is 7, and in bacterium solution, the density of actinomyces naeslundii is 105 CFU/mL；Second step, is added by every 100 grams of cupro-nickel tailings
The ratio of 30mL bacterium solutions, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying environment
Lower culture 12 days, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 10：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, will
Actinomyces naeslundiiActinomyces naeslundiiThe mass percent for being placed in urea is 10%, the concentration of calcium ion is 1.5 to rub
You/liter nutrient broth nutrient solution in cultivate 2 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth culture
Liquid and the pH of bacterium solution are 7, and in bacterium solution, the density of actinomyces naeslundii is 105 CFU/mL；Second step, by every 100 grams of cupro-nickel tailings
The ratio of 50mL bacterium solutions is added, bacterium solution is sprayed in cupro-nickel tailing, the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying
Cultivated 12 days under environment, then after natural air drying, obtain curing cupro-nickel tailing.
Embodiment 11：Application of the actinomyces naeslundii in cupro-nickel tailing solidifying, carries out as follows：The first step, will
Actinomyces naeslundiiActinomyces naeslundiiThe mass percent for being placed in urea is 12%, the concentration of calcium ion is 1.5 to rub
You/liter nutrient broth nutrient solution in cultivate 2 days after obtain the bacterium solution with induced curing effect, wherein, nutrient broth culture
Liquid and the pH of bacterium solution are 9, and in bacterium solution, the density of actinomyces naeslundii is 105 CFU/mL；Second step, by every 100 grams of cupro-nickel tailings
The ratio of 120mL bacterium solutions is added, bacterium solution is sprayed in cupro-nickel tailing, the cupro-nickel tailing after sprinkling bacterium solution is placed in natural drying
Cultivated 12 days under environment, then after natural air drying, obtain curing cupro-nickel tailing.
By table 1, curing cupro-nickel tail that the method described in embodiment 3 to embodiment 11 obtains cupro-nickel tailings glass
The tolerance wind-force of ore deposit reaches more than 30 meter per seconds, compressive resistance reaches more than 38kPa, the fixed rate of nickel ion reach 10.3% with
On, the fixed rate of copper ion reaches more than 8.5%.
In conclusion application of the first public actinomyces naeslundii of the present invention in cupro-nickel tailing solidifying, passes through Nei Shi unwrapping wire
The biological curing effect of bacterium cures cupro-nickel tailings particles, makes its curing blocking, while by copper ion, nickel ion etc. in cupro-nickel tailing
Heavy metal ion is also secured in its block, after curing process, since cupro-nickel tailing is in bulk, and, it is resistant to wind power energy
Power and compressive resistance greatly improve, and make it when meeting with strong wind weather, will not be scraped it is scattered, also it is avoided that causing large-scale environment
Pollution, meanwhile, substantially reduce the heavy metal ion in cupro-nickel tailing and radioactive substance and flow into river nearby and permeate the ground
Amount, that is, significantly reduce it and polluted caused by river and underground water and radiolesion, furthermore it is possible to significantly reduce cupro-nickel tailing
Occupancy and destruction to land resource, based on the above, application of the actinomyces naeslundii in cupro-nickel tailing solidifying, have ecology
Protection significance.
Above technical characteristic constitutes the embodiment of the present invention, it, can basis with stronger adaptability and implementation result
It is actually needed and increases and decreases non-essential technical characteristic, meets the needs of different situations.
Claims (3)
- A kind of 1. application of actinomyces naeslundii in cupro-nickel tailing solidifying.
- 2. application of the actinomyces naeslundii according to claim 1 in cupro-nickel tailing solidifying, it is characterised in that by following sides Method carries out：The first step, by actinomyces naeslundiiActinomyces naeslundiiBe placed in the mass percent of urea for 2% to 10%th, the concentration of calcium ion is to be obtained after being cultivated 1 day to 3 days in the nutrient broth nutrient solution of 0.2 mol/L to 1.5 mol/Ls Bacterium solution with induced curing effect, wherein, the pH of nutrient broth nutrient solution and bacterium solution is 5 to 10, in bacterium solution, Nei Shi unwrapping wire The density of bacterium is 104 CFU/mL to 108 CFU/mL；Second step, 30mL is added to 120mL bacterium solutions by every 100 grams of cupro-nickel tailings Ratio, bacterium solution is sprayed in cupro-nickel tailing, and the cupro-nickel tailing after sprinkling bacterium solution is placed in spontaneously dry and is cultivated 10 days under environment To 15 days, then after natural air drying, obtain curing cupro-nickel tailing.
- 3. application of the actinomyces naeslundii according to claim 2 in cupro-nickel tailing solidifying, it is characterised in that bacterium solution is pressed The method of stating obtains：Actinomyces naeslundii bacterial strain is placed in nutrient broth medium after recovering, is forwarded to the nutritious soup that pH is 5 to 10 In culture medium, temperature be 30 DEG C under conditions of culture 48 it is small when after obtain bacterium solution.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109047269A (en) * | 2018-06-19 | 2018-12-21 | 浙江工业大学 | Solidify the method for the cementing industrial residue of cobalt containing heavy metal using Staphylococcus cohnis solution urea subspecies |
| CN109456770A (en) * | 2018-11-23 | 2019-03-12 | 国网新疆电力有限公司电力科学研究院 | Application of the hydrolysis of urea bacterium of salt tolerant in Pb-Zn tailings solidify |
| CN110576023A (en) * | 2019-07-26 | 2019-12-17 | 国网新疆电力有限公司电力科学研究院 | Application of Actinomyces naeslundii in solidification of copper-molybdenum tailings |
| CN111534689A (en) * | 2020-03-20 | 2020-08-14 | 浙江工业大学 | Method for mineralizing and fixing heavy metal in tailings by utilizing fusiform lysine bacillus and quicklime |
| CN112077122A (en) * | 2020-09-07 | 2020-12-15 | 国网新疆电力有限公司电力科学研究院 | Method for solidifying iron tailings by inducing solidification effect bacteria |
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| CN109047269A (en) * | 2018-06-19 | 2018-12-21 | 浙江工业大学 | Solidify the method for the cementing industrial residue of cobalt containing heavy metal using Staphylococcus cohnis solution urea subspecies |
| CN109456770A (en) * | 2018-11-23 | 2019-03-12 | 国网新疆电力有限公司电力科学研究院 | Application of the hydrolysis of urea bacterium of salt tolerant in Pb-Zn tailings solidify |
| CN110576023A (en) * | 2019-07-26 | 2019-12-17 | 国网新疆电力有限公司电力科学研究院 | Application of Actinomyces naeslundii in solidification of copper-molybdenum tailings |
| CN111534689A (en) * | 2020-03-20 | 2020-08-14 | 浙江工业大学 | Method for mineralizing and fixing heavy metal in tailings by utilizing fusiform lysine bacillus and quicklime |
| CN112077122A (en) * | 2020-09-07 | 2020-12-15 | 国网新疆电力有限公司电力科学研究院 | Method for solidifying iron tailings by inducing solidification effect bacteria |
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