CN104255516A - Rapid propagation method for cortex dictamni regeneration plant - Google Patents
Rapid propagation method for cortex dictamni regeneration plant Download PDFInfo
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- CN104255516A CN104255516A CN201410540510.8A CN201410540510A CN104255516A CN 104255516 A CN104255516 A CN 104255516A CN 201410540510 A CN201410540510 A CN 201410540510A CN 104255516 A CN104255516 A CN 104255516A
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- shaggy
- fruited dittany
- root bark
- temperature
- root
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Abstract
The invention discloses a rapid propagation method for a cortex dictamni regeneration plant. The method comprises steps including obtaining of sterile cortex dictamni blades, callus induction, callus differentiation, root induction, acclimatization and transplanting, and the like. According to the method, a variety with desirable traits including disease resistance, salt resistance, insect resistance and the like can be directly obtained through mutagenesis and screening by adopting tissue culture, germplasm resources are stored, and loss of good genes is prevented.
Description
Technical field
The present invention relates to the quick-breeding method of root bark of shaggy-fruited dittany tissue cultures, belong to plant technology field.
Background technology
The root bark of shaggy-fruited dittany,
cortex dictamni, also known as dittany bark, stereotyped writing ox, seguin argyreia herb, the fresh grass of sheep, Rutaceae, perennial herb, in being born in mountain region shrub and under forest, hillside tailo.In shaggy-fruited dittany is born in slight slope shrub, in the shrub of mountain region and under forest, hillside tailo.Shaggy-fruited dittany likes warm and moist, cold-resistant, is afraid of drought, is afraid of flood, is afraid of intense light irradiation.Northeast, North China, East China and Shaanxi, Gansu, Henan, Sichuan, Guizhou, Xinjiang, maximum with Yi Li, Altay Prefecture.Root bark of shaggy-fruited dittany acrial part contains psoralen, xanthotoxin, Scopoletin, Quercetin, isoquercitin, root contains dictamine, γ-fagarine, front skimmianine, skimmianine, dasycarpamin, trigonelline, choline, O-ethyl-fall-dictamine, O-ethyl-fall-γ-fagarine, O-ethyl-fall-skimmianine, different spot boils woods potash, evodine, shaggy-fruited dittany alcohol, pregnene acid ketone, fraxinellon, obakunone, limonin, cupreol, Lay oil sterol, saponin etc., function: heat-clearing and damp-drying drug; Dispelling wind and arresting itching; Removing toxic substances, cures mainly: wind-heat wets the rubella caused by poison; Eczema; Mange; Jaundice; Damp-heat arthralgia.Mainly adopt stem tuber breeding at present, tissue culture technology there is no research.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of method for quickly breeding of root bark of shaggy-fruited dittany plant regeneration, the inventive method adopts tissue cultures can directly mutagenesis and screen the kind providing disease-resistant, anti-salt, the merit such as pest-resistant, preserve germ plasm resource, avoid the loss of excellent genes.
Technical problem to be solved by this invention is realized by following scheme:
Get the blade that root bark of shaggy-fruited dittany children is tender, running water 23min, on superclean bench, clorox disinfects 8min, aseptic water washing 6 times, in the inducing culture of the root bark of shaggy-fruited dittany young leaflet tablet access RM+6-BA1.3mg/L+NAA0.2mg/L+0.7% agar+30g/L sucrose of disinfecting, be placed in constant temperature water box and carry out the process of high temperature thermal shock, 36 DEG C of thermal shock process 20h, take out the induction carrying out callus, pH5.8, dark illumination cultivation, temperature 23 ± 2 DEG C, the callus derived is put into medium RM+NAA0.8-1.2mg/L+ZT2.0-2.5mg/L and is carried out Calli Differentiation cultivation, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 2200lx, temperature 24 ± 2 DEG C, root induction is carried out in differentiation bud seedling access 1/2MS medium out, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 5000lx, temperature 26 ± 2 DEG C, treat that test-tube plantlet is about 5cm, when root is about 2cm, take out the peat put into high-temperature sterilization and cross: the matrix of broken haydite=3:1, use high pressure atomizing device, keep indoor air relative humidity 90%, carbon dioxide generating device keeps gas concentration lwevel to be 500ppm, auto spraying is regularly sprayed water, mancozeb 90mg/L is sprayed every 15 days, spray 3/4 rural standard liquid nutrient every three days, temperature controls at 25 DEG C, to shelter from heat or light rate 65%, survival rate is added up after one month.
The root bark of shaggy-fruited dittany survival rate adopting the present invention to prepare is higher, and the cycle is short, and output is large, and less energy consumption, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
Get the blade that root bark of shaggy-fruited dittany children is tender, running water 23min, on superclean bench, clorox disinfects 8min, aseptic water washing 6 times, in the inducing culture of the root bark of shaggy-fruited dittany young leaflet tablet access RM+6-BA1.3mg/L+NAA0.2mg/L+0.7% agar+30g/L sucrose of disinfecting, be placed in constant temperature water box and carry out the process of high temperature thermal shock, 36 DEG C of thermal shock process 20h, take out the induction carrying out callus, pH5.8, dark illumination cultivation, temperature 23 ± 2 DEG C, the callus derived is put into medium RM+NAA0.8mg/L+ZT2.0mg/L and is carried out Calli Differentiation cultivation, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 2200lx, temperature 24 ± 2 DEG C, root induction is carried out in differentiation bud seedling access 1/2MS medium out, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 5000lx, temperature 26 ± 2 DEG C, treat that test-tube plantlet is about 5cm, when root is about 2cm, take out the peat put into high-temperature sterilization and cross: the matrix of broken haydite=3:1, use high pressure atomizing device, keep indoor air relative humidity 90%, carbon dioxide generating device keeps gas concentration lwevel to be 500ppm, auto spraying is regularly sprayed water, mancozeb 90mg/L is sprayed every 15 days, spray 3/4 rural standard liquid nutrient every three days, temperature controls at 25 DEG C, to shelter from heat or light rate 65%, survival rate is added up after one month, survival rate 89%.
Embodiment 2
Get the blade that root bark of shaggy-fruited dittany children is tender, running water 23min, on superclean bench, clorox disinfects 8min, aseptic water washing 6 times, in the inducing culture of the root bark of shaggy-fruited dittany young leaflet tablet access RM+6-BA1.3mg/L+NAA0.2mg/L+0.7% agar+30g/L sucrose of disinfecting, be placed in constant temperature water box and carry out the process of high temperature thermal shock, 36 DEG C of thermal shock process 20h, take out the induction carrying out callus, pH5.8, dark illumination cultivation, temperature 23 ± 2 DEG C, the callus derived is put into medium RM+NAA1.2mg/L+ZT2.5mg/L and is carried out Calli Differentiation cultivation, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 2200lx, temperature 24 ± 2 DEG C, root induction is carried out in differentiation bud seedling access 1/2MS medium out, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 5000lx, temperature 26 ± 2 DEG C, treat that test-tube plantlet is about 5cm, when root is about 2cm, take out the peat put into high-temperature sterilization and cross: the matrix of broken haydite=3:1, use high pressure atomizing device, keep indoor air relative humidity 90%, carbon dioxide generating device keeps gas concentration lwevel to be 500ppm, auto spraying is regularly sprayed water, mancozeb 90mg/L is sprayed every 15 days, spray 3/4 rural standard liquid nutrient every three days, temperature controls at 25 DEG C, to shelter from heat or light rate 65%, survival rate is added up after one month, survival rate 88%.
Embodiment 3
Get the blade that root bark of shaggy-fruited dittany children is tender, running water 23min, on superclean bench, clorox disinfects 8min, aseptic water washing 6 times, in the inducing culture of the root bark of shaggy-fruited dittany young leaflet tablet access RM+6-BA1.3mg/L+NAA0.2mg/L+0.7% agar+30g/L sucrose of disinfecting, be placed in constant temperature water box and carry out the process of high temperature thermal shock, 36 DEG C of thermal shock process 20h, take out the induction carrying out callus, pH5.8, dark illumination cultivation, temperature 23 ± 2 DEG C, the callus derived is put into medium RM+NAA1.2mg/L+ZT2.0mg/L and is carried out Calli Differentiation cultivation, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 2200lx, temperature 24 ± 2 DEG C, root induction is carried out in differentiation bud seedling access 1/2MS medium out, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 5000lx, temperature 26 ± 2 DEG C, treat that test-tube plantlet is about 5cm, when root is about 2cm, take out the peat put into high-temperature sterilization and cross: the matrix of broken haydite=3:1, use high pressure atomizing device, keep indoor air relative humidity 90%, carbon dioxide generating device keeps gas concentration lwevel to be 500ppm, auto spraying is regularly sprayed water, mancozeb 90mg/L is sprayed every 15 days, spray 3/4 rural standard liquid nutrient every three days, temperature controls at 25 DEG C, to shelter from heat or light rate 65%, survival rate is added up after one month, survival rate 90%.
Claims (3)
1. a method for quickly breeding for root bark of shaggy-fruited dittany regeneration plant, comprise the acquisition of the aseptic blade of the root bark of shaggy-fruited dittany, the induction of callus, the differentiation of callus, root induction, acclimatization and transplants, its key step is as follows:
(1) blade that root bark of shaggy-fruited dittany children is tender is got, to its disinfection;
(2) get in the inducing culture of the root bark of shaggy-fruited dittany young leaflet tablet access RM+6-BA1.3mg/L+NAA0.2mg/L+0.7% agar+30g/L sucrose that step (1) was disinfected, be placed in constant temperature water box and carry out the process of high temperature thermal shock, 36 DEG C of thermal shock process 20h, take out the induction carrying out callus, pH5.8, dark illumination cultivation, temperature 23 ± 2 DEG C;
(3) get callus that step (2) derives to put into medium RM+NAA0.8-1.2mg/L+ZT2.0-2.5mg/L and carry out Calli Differentiation cultivation, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 2200lx, temperature 24 ± 2 DEG C;
(4) get in step (3) differentiation bud seedling access 1/2MS medium out and carry out root induction, additional saccharose 30g/L, agar 7g/L, pH5.8, illumination 5000lx, temperature 26 ± 2 DEG C;
(5) test-tube plantlet getting step (4) carries out acclimatization and transplants.
2. according to the method for quickly breeding of a kind of root bark of shaggy-fruited dittany regeneration plant according to claim 1, it is characterized in that: the acquisition of the aseptic blade of the root bark of shaggy-fruited dittany described in step (1) is, get the blade that root bark of shaggy-fruited dittany children is tender, running water 23min, on superclean bench, clorox disinfects 8min, aseptic water washing 6 times.
3. according to the method for quickly breeding of a kind of root bark of shaggy-fruited dittany regeneration plant according to claim 1, it is characterized in that: in step (5), the method for root bark of shaggy-fruited dittany acclimatization and transplants is for treating that test-tube plantlet is about 5cm, when root is about 2cm, take out the peat put into high-temperature sterilization and cross: the matrix of broken haydite=3:1, use high pressure atomizing device, keep indoor air relative humidity 90%, carbon dioxide generating device keeps gas concentration lwevel to be 500ppm, auto spraying is regularly sprayed water, mancozeb 90mg/L is sprayed every 15 days, spray 3/4 rural standard liquid nutrient every three days, temperature controls at 25 DEG C, to shelter from heat or light rate 65%, survival rate is added up after one month.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410540510.8A CN104255516A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for cortex dictamni regeneration plant |
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| CN201410540510.8A CN104255516A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for cortex dictamni regeneration plant |
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| CN201410540510.8A Pending CN104255516A (en) | 2014-10-14 | 2014-10-14 | Rapid propagation method for cortex dictamni regeneration plant |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104718935A (en) * | 2015-02-16 | 2015-06-24 | 哈尔滨市合和中药材种植有限公司 | Traditional Chinese medicine dictamnus dasycarpus artificial seedling method |
| CN113575309A (en) * | 2021-08-23 | 2021-11-02 | 东北林业大学 | Intercropping dimming method for increasing fraxinellone content in root of densefruit pittany |
-
2014
- 2014-10-14 CN CN201410540510.8A patent/CN104255516A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104718935A (en) * | 2015-02-16 | 2015-06-24 | 哈尔滨市合和中药材种植有限公司 | Traditional Chinese medicine dictamnus dasycarpus artificial seedling method |
| CN113575309A (en) * | 2021-08-23 | 2021-11-02 | 东北林业大学 | Intercropping dimming method for increasing fraxinellone content in root of densefruit pittany |
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Application publication date: 20150107 |