CN104232477B - Biological sample asepticization sampler - Google Patents
Biological sample asepticization sampler Download PDFInfo
- Publication number
- CN104232477B CN104232477B CN201410464751.9A CN201410464751A CN104232477B CN 104232477 B CN104232477 B CN 104232477B CN 201410464751 A CN201410464751 A CN 201410464751A CN 104232477 B CN104232477 B CN 104232477B
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- CN
- China
- Prior art keywords
- hollow fiber
- film assembly
- inlet pipe
- fiber film
- air inlet
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Abstract
The present invention provides a kind of biological sample asepticization sampler, including hollow fiber film assembly, air supply unit and sampling cutter, hollow fiber film assembly has air inlet pipe and gas outlet, and sampling cutter is fixed on gas outlet place, and air supply unit is connected with air inlet pipe；Hollow fiber film assembly includes hollow fiber hydrophobic membrane, and the internal diameter of hollow fiber hydrophobic membrane is 0.8+_ 0.5mm；Film silk thickness is 0.15mm；Porosity is 85%；Aperture is 0.1-0.16um。The invention has the beneficial effects as follows the air inlet pipe external air feed equipment of energy in this device, it is achieved the function of isolation microbes in air antibacterial during biological sample sampling。
Description
Technical field
The present invention relates to a kind of biological sample asepticization sampler。
Background technology
In existing technology, field acquisition strain is very difficult, it is generally all the slant medium carrying out test tube, it is placed on after autoclaving in travelling bag, generally all it is with 300 test tubes or more, under environment in the wild, condition is simple and crude, is typically all the miscellaneous bacteria carrying out air-isolation weight by the mode lighting alcohol burner, generally the local sampling of the boundary of the cap of wild mushroom and bacterium bar is placed in test tube。But survival rate is very low, going to Pubei County, Guangxi to gather red cone bacterium when the author's part in may, 300 test tubes taken only have survived 1, it is seen that the effect surviving key smoothly of the miscellaneous bacteria docking bacterium in the air of field。
Summary of the invention
The problem to be solved in the present invention is to provide a kind of biological sample asepticization sampler, overcomes deficiency of the prior art。
Adopt hollow fiber hydrophobic membrane that air is filtered, hollow fiber hydrophobic membrane is the film of permeable watertight, therefore film cannot be passed through owing to the miscellaneous bacteria diameter in air is all higher than the aperture of hollow fiber hydrophobic membrane, the air inlet pipe of hollow fiber hydrophobic membrane is supplied by the easy device that can utilize ear washing bulb or continuous gas entry, aseptic dry air is obtained from gas outlet, what aseptic dry air was lasting shrouds wild thalline, artificial creation gnotobasis, carrying out connecing bacterium so in an aseptic environment, microbiological contamination rate just can significantly reduce。
For reaching above-mentioned purpose, the technical solution used in the present invention is: biological sample asepticization sampler, including hollow fiber film assembly, air supply unit and sampling cutter,
Described hollow fiber film assembly has air inlet pipe and gas outlet, described air inlet pipe is connected on described hollow fiber film assembly in the side port of one end thereof, described air inlet pipe and described hollow fiber film assembly internal run-through, described gas outlet is positioned on another end face of described hollow fiber film assembly, and on described hollow fiber film assembly, the part except described air inlet pipe and described gas outlet is enclosed construction；
Described sampling cutter is fixed on described gas outlet place, and described air supply unit is connected with described air inlet pipe；
Described hollow fiber film assembly includes hollow fiber hydrophobic membrane, and the internal diameter of described hollow fiber hydrophobic membrane is 0.8mm；Film silk thickness is 0.15mm；Porosity is 85%；Aperture is 0.1-0.16um。
Further, described sampling cutter is tubulose cutter hub, the front end face of described tubulose cutter hub is beveled edge, the rear end of described tubulose cutter hub is fixed on described gas outlet, and the side of described tubulose cutter hub has a sideslip mouth, has pushing block in described tubulose cutter hub, described pushing block side has push handle, described pushing block side is fixed in described push handle one end, and the other end stretches out described sideslip mouth, and the cross-sectional area of described pushing block is less than the cross-sectional area of described tubulose cutter hub。
Further, described Tubular tool side has multiple aperture near described beveled edge place。
Further, described air supply unit includes ear washing bulb, air bag, air pump or aerator。
The present invention has the advantage that and has the benefit effect that owing to adopting technique scheme, simple in construction, it is simple to carry, and realizes sterile working during sampling, and strain survival rate is high。
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the present invention；
In figure: 1-hollow fiber film assembly；2-tubulose cutter hub；3-air inlet pipe；4-beveled edge；5-sideslip mouth；6-pushing block；7-push handle；8-aperture；Arrow trend is moved towards for air-flow。
Detailed description of the invention
As it is shown in figure 1, the present invention provides a kind of biological sample asepticization sampler, including hollow fiber film assembly 1, air supply unit (not shown) and sampling cutter,
Hollow fiber film assembly 1 has air inlet pipe and gas outlet (not shown), air inlet pipe 3 is connected on hollow fiber film assembly 1 in the side port of one end thereof, air inlet pipe 3 and hollow fiber film assembly 1 internal run-through, gas outlet is positioned on another end face of hollow fiber film assembly 1, and on hollow fiber film assembly 1, the part except air inlet pipe 3 and gas outlet is enclosed construction；
Sampling cutter is fixed on gas outlet 8 place, and air supply unit is connected with air inlet pipe 3；Air supply unit can select the device of any conveying air such as ear ball, air bag, air pump or aerator；
Hollow fiber film assembly 1 includes hollow fiber hydrophobic membrane, and the internal diameter of hollow fiber hydrophobic membrane is 0.8mm；Film silk thickness is 0.15mm；Porosity is 85%；Aperture is 0.16um；Film silk number is 25-200；Membrane module length is preferably 21cm；Membrane module internal diameter is preferably 3cm。
During use, air inlet pipe 3 is connected with air supply unit; pass air through hollow fiber film assembly 1; owing to hollow fiber film assembly 1 can by the diameter of the microbial bacterial in the air aperture more than hollow fiber hydrophobic membrane; therefore retained by hollow fiber hydrophobic membrane; from the gas outlet 8 of hollow fiber film assembly 1 air out be purify after aseptic dry air; then purge gas is full of sampling cutter and neighboring area thereof always; when this allows for sampling, sample is in the protection of purge gas all the time; will not being subject to the pollution of normal air around, sample survival rate is high。
Sampling cutter is preferably tubulose cutter hub 2, and the front end face of tubulose cutter hub 2 is beveled edge 4, and the rear end of tubulose cutter hub 2 is fixed on gas outlet 8；Sample enters in tubulose cutter hub 2 after being cut by beveled edge 4；
In order to facilitate the taking-up of sample in tubulose cutter hub 2, the side of tubulose cutter hub 2 has a sideslip mouth 5, there is in tubulose cutter hub 2 pushing block 6, pushing block 6 side has push handle 7, and pushing block 6 side is fixed in push handle 7 one end, and the other end stretches out sideslip mouth 5, the cross-sectional area of pushing block 6 is less than the cross-sectional area of tubulose cutter hub 2, will not hindering the circulation of purge gas, only need to promote push handle 7 when needing to take out sample after sampling, sample is ejected tubulose cutter hub 2 by manipulation pushing block 6。Tubular tool 2 side has multiple aperture 8 near beveled edge 4 place。The setting of aperture 8 makes that the coverage of the filtrated air around sample is bigger, air is cleaner。
Above embodiments of the invention are described in detail, but described content has been only presently preferred embodiments of the present invention, it is impossible to be considered the practical range for limiting the present invention。All equalizations made according to the present patent application scope change and improvement etc., all should still belong within the patent covering scope of the present invention。
Claims (3)
1. biological sample asepticization sampler, it is characterized in that: include hollow fiber film assembly, air supply unit and sampling cutter, described hollow fiber film assembly has air inlet pipe and gas outlet, described air inlet pipe is connected on described hollow fiber film assembly in the side port of one end thereof, described air inlet pipe and described hollow fiber film assembly internal run-through, described gas outlet is positioned on another end face of described hollow fiber film assembly, and on described hollow fiber film assembly, the part except described air inlet pipe and described gas outlet is enclosed construction；
Described sampling cutter is fixed on described gas outlet place, and described air supply unit is connected with described air inlet pipe；
Described hollow fiber film assembly includes hollow fiber hydrophobic membrane, and the internal diameter of described hollow fiber hydrophobic membrane is 0.8mm；Film silk thickness is 0.15mm；Porosity is 85%；Aperture is 0.1-0.16 μm；
Described sampling cutter is tubulose cutter hub, the front end face of described tubulose cutter hub is beveled edge, the rear end of described tubulose cutter hub is fixed on described gas outlet, the side of described tubulose cutter hub has a sideslip mouth, having pushing block in described tubulose cutter hub, described pushing block side has push handle, and described pushing block side is fixed in described push handle one end, the other end stretches out described sideslip mouth, and the cross-sectional area of described pushing block is less than the cross-sectional area of described tubulose cutter hub。
2. biological sample asepticization sampler according to claim 1, it is characterised in that: described Tubular tool side has multiple aperture near described beveled edge place。
3. the biological sample asepticization sampler according to any one of claim 1 or 2, it is characterised in that: described air supply unit includes ear washing bulb, air bag, air pump or aerator。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410464751.9A CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410464751.9A CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104232477A CN104232477A (en) | 2014-12-24 |
| CN104232477B true CN104232477B (en) | 2016-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410464751.9A Active CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3257585A1 (en) * | 2016-06-16 | 2017-12-20 | bioMérieux | Tip and device for sampling colonies of microorganisms and sampling method implementing same |
| CN112048439B (en) * | 2020-07-27 | 2022-05-17 | 中国科学院广州能源研究所 | Sampling and culturing device for preventing disturbance of marine microorganism system for experiment |
| CN114149900B (en) * | 2021-11-30 | 2024-03-29 | 中国科学院天津工业生物技术研究所 | Cutting head and mycelium cutting method |
Citations (4)
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|---|---|---|---|---|
| CN101057749A (en) * | 2006-04-17 | 2007-10-24 | 黄樟焱 | Novel clever seat with preventing secondary pollution function |
| CN102114390A (en) * | 2009-12-30 | 2011-07-06 | 中国科学院生态环境研究中心 | Reinforced type polyvinylidene fluoride hollow fiber hydrophobic membrane and preparation method thereof |
| CN102304471A (en) * | 2011-08-18 | 2012-01-04 | 深圳国际旅行卫生保健中心 | Liquid filtration-type air microbial sampler and sampling system |
| CN103908898A (en) * | 2013-01-07 | 2014-07-09 | 吕晓龙 | Composite hollow fiber film and making method thereof |
-
2014
- 2014-09-12 CN CN201410464751.9A patent/CN104232477B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101057749A (en) * | 2006-04-17 | 2007-10-24 | 黄樟焱 | Novel clever seat with preventing secondary pollution function |
| CN102114390A (en) * | 2009-12-30 | 2011-07-06 | 中国科学院生态环境研究中心 | Reinforced type polyvinylidene fluoride hollow fiber hydrophobic membrane and preparation method thereof |
| CN102304471A (en) * | 2011-08-18 | 2012-01-04 | 深圳国际旅行卫生保健中心 | Liquid filtration-type air microbial sampler and sampling system |
| CN103908898A (en) * | 2013-01-07 | 2014-07-09 | 吕晓龙 | Composite hollow fiber film and making method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 中空纤维膜技术及其应用;吕经烈;《海洋技术》;20021231;第21卷(第4期);73-76 * |
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| CN104232477A (en) | 2014-12-24 |
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