CN104232477A - Aseptic sampling device for biological sample - Google Patents
Aseptic sampling device for biological sample Download PDFInfo
- Publication number
- CN104232477A CN104232477A CN201410464751.9A CN201410464751A CN104232477A CN 104232477 A CN104232477 A CN 104232477A CN 201410464751 A CN201410464751 A CN 201410464751A CN 104232477 A CN104232477 A CN 104232477A
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- China
- Prior art keywords
- hollow fiber
- inlet pipe
- film assembly
- sampling
- biological sample
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
Abstract
The invention provides an aseptic sampling device for a biological sample. The aseptic sampling device comprises a hollow fiber membrane module, an air supply unit and a sampling tool, wherein an air inlet pipe and an air outlet are formed in the hollow fiber membrane module; the sampling tool is fixed on the air outlet; the air supply unit is connected with the air inlet pipe; the hollow fiber membrane module comprises a hollow fiber hydrophobic membrane; the inner diameter of the hollow fiber hydrophobic membrane is 0.8+/-0.5mm; the thickness of a membrane fiber is 0.15mm; the porosity is 85%; and the aperture is 0.1-0.16mu m. The aseptic sampling device has the beneficial effects that the air inlet pipe in the device can be externally connected with air supply equipment, so that the functions of extremophile bacteria in isolated air during the period of sampling the biological sample are achieved.
Description
Technical field
The present invention relates to a kind of biological sample asepticize sampling unit.
Background technology
In existing technology, field acquisition bacterial classification is very difficult, usually be all the slant medium carrying out test tube, be placed on after autoclaving in travelling bag, general 300 test tubes or more be all with, under environment in the wild, condition is simple and crude, is all generally to carry out the heavy miscellaneous bacteria of air-isolation by the mode of lighting spirit lamp, is usually placed in test tube the place sampling of the cap of wild mushroom and the boundary of bacterium bar.But surviving rate is very low, the author in may part time go to Pubei County, Guangxi to gather red cone bacterium, 300 test tubes of taking only have survived 1, the effect surviving key smoothly of miscellaneous bacteria in the air of visible field docking bacterium.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of biological sample asepticize sampling unit, overcomes deficiency of the prior art.
Hollow fiber hydrophobic membrane is adopted to filter air, hollow fiber hydrophobic membrane is the film of permeable watertight, film cannot be passed through in the aperture being all greater than hollow fiber hydrophobic membrane due to the miscellaneous bacteria diameter in air therefore, the easy device of rubber suction bulb or continuous gas entry can be utilized the inlet pipe air feed of hollow fiber hydrophobic membrane, the air of aseptic drying is obtained from air outlet, what the air of aseptic drying continued shrouds wild thalline, artificial creation gnotobasis, carry out so in an aseptic environment connecing bacterium, microbiological contamination rate just can significantly reduce.
For achieving the above object, the technical solution used in the present invention is: biological sample asepticize sampling unit, comprises hollow fiber film assembly, air supply unit and sampling cutter,
Described hollow fiber film assembly have inlet pipe and air outlet, described inlet pipe is connected on the side mouth of close one end thereof on described hollow fiber film assembly, described inlet pipe and described hollow fiber film assembly internal run-through, described air outlet is positioned on another end face of described hollow fiber film assembly, and the part on described hollow fiber film assembly except described inlet pipe and described air outlet is enclosed construction;
Described sampling cutter is fixed on described air outlet place, and described air supply unit is connected with described inlet pipe;
Described hollow fiber film assembly comprises hollow fiber hydrophobic membrane, and the internal diameter of described hollow fiber hydrophobic membrane is 0.8
+_ 0.5mm; Film silk thickness is 0.15mm; Porosity is 85%; Aperture is 0.1-0.16um.
Further, described sampling cutter is tubulose cutter hub, and the front end face of described tubulose cutter hub is beveled edge, and the rear end of described tubulose cutter hub is fixed on described air outlet.
Further, the side of described tubulose cutter hub has a sideslip mouth, in described tubulose cutter hub, there is pushing block, described pushing block side has and pushes away handle, described pushing block side is fixed in the described handle one end that pushes away, the other end stretches out described sideslip mouth, and the cross-sectional area of described pushing block is less than the cross-sectional area of described tubulose cutter hub.
Further, described Tubular tool side has multiple aperture near described beveled edge place.
Further, described air supply unit comprises rubber suction bulb, air bag, air pump or gas blower.
The advantage that the present invention has and positively effect are: owing to adopting technique scheme, structure is simple, and be easy to carry, realize aseptic technique during sampling, bacterial classification surviving rate is high.
Accompanying drawing explanation
Fig. 1 is one-piece construction schematic diagram of the present invention;
In figure: 1-hollow fiber film assembly; 2-tubulose cutter hub; 3-inlet pipe; 4-beveled edge; 5-sideslip mouth; 6-pushing block; 7-pushes away handle; 8-aperture; Arrow trend is air-flow trend.
Embodiment
As shown in Figure 1, the invention provides a kind of biological sample asepticize sampling unit, comprise hollow fiber film assembly 1, air supply unit (not shown) and sampling cutter,
Hollow fiber film assembly 1 have inlet pipe and air outlet (not shown), inlet pipe 3 is connected on the side mouth of close one end thereof on hollow fiber film assembly 1, inlet pipe 3 and hollow fiber film assembly 1 internal run-through, air outlet is positioned on another end face of hollow fiber film assembly 1, and the part on hollow fiber film assembly 1 except inlet pipe 3 and air outlet is enclosed construction;
Sampling cutter is fixed on air outlet 8 place, and air supply unit is connected with inlet pipe 3; Air supply unit can select the device of any conveying air such as ear ball, air bag, air pump or gas blower;
Hollow fiber film assembly 1 comprises hollow fiber hydrophobic membrane, and the internal diameter of hollow fiber hydrophobic membrane is 0.8mm; Film silk thickness is 0.15mm; Porosity is 85%; Aperture is 0.16um; Film silk number is 25-200; Membrane module length is preferably 21cm; Membrane module internal diameter is preferably 3cm.
During use, inlet pipe 3 is connected with air supply unit; air is made to pass hollow fiber film assembly 1; because the diameter of the microbial bacterial in air can be greater than the aperture of hollow fiber hydrophobic membrane by hollow fiber film assembly 1; therefore retained by hollow fiber hydrophobic membrane; dry air aseptic after being purification from air outlet 8 air out of hollow fiber film assembly 1; then purified gas is full of sampling cutter and neighboring area thereof always; when this just makes to sample, sample is in the protection of purified gas all the time; can not be subject to the pollution of normal air around, sample surviving rate is high.
Sampling cutter is preferably tubulose cutter hub 2, and the front end face of tubulose cutter hub 2 is beveled edge 4, and the rear end of tubulose cutter hub 2 is fixed on air outlet 8; Sample enters in tubulose cutter hub 2 after being cut by beveled edge 4;
The conveniently taking-up of sample in tubulose cutter hub 2, the side of tubulose cutter hub 2 has a sideslip mouth 5, there is in tubulose cutter hub 2 pushing block 6, pushing block 6 side has and pushes away handle 7, and push away handle 7 one end and be fixed on pushing block 6 side, the other end stretches out sideslip mouth 5, the cross-sectional area of pushing block 6 is less than the cross-sectional area of tubulose cutter hub 2, can not hinder the circulation of purified gas, when needing only need to promote handle 7 when taking out sample after sampling, sample is ejected tubulose cutter hub 2 by manipulation pushing block 6.Tubular tool 2 side has multiple aperture 8 near beveled edge 4 place.The setting of aperture 8 makes that the coverage of the sterile air around sample is larger, air is cleaner.
Above embodiments of the invention have been described in detail, but described content being only preferred embodiment of the present invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the present patent application scope change and improve, and all should still belong within patent covering scope of the present invention.
Claims (5)
1. biological sample asepticize sampling unit, is characterized in that: comprise hollow fiber film assembly, air supply unit and sampling cutter,
Described hollow fiber film assembly have inlet pipe and air outlet, described inlet pipe is connected on the side mouth of close one end thereof on described hollow fiber film assembly, described inlet pipe and described hollow fiber film assembly internal run-through, described air outlet is positioned on another end face of described hollow fiber film assembly, and the part on described hollow fiber film assembly except described inlet pipe and described air outlet is enclosed construction;
Described sampling cutter is fixed on described air outlet place, and described air supply unit is connected with described inlet pipe;
Described hollow fiber film assembly comprises hollow fiber hydrophobic membrane, and the internal diameter of described hollow fiber hydrophobic membrane is 0.8
+_ 0.5mm; Film silk thickness is 0.15mm; Porosity is 85%; Aperture is 0.1-0.16um.
2. biological sample asepticize sampling unit according to claim 1, is characterized in that: described sampling cutter is tubulose cutter hub, the front end face of described tubulose cutter hub is beveled edge, and the rear end of described tubulose cutter hub is fixed on described air outlet.
3. biological sample asepticize sampling unit according to claim 2, it is characterized in that: the side of described tubulose cutter hub has a sideslip mouth, in described tubulose cutter hub, there is pushing block, described pushing block side has and pushes away handle, described pushing block side is fixed in the described handle one end that pushes away, the other end stretches out described sideslip mouth, and the cross-sectional area of described pushing block is less than the cross-sectional area of described tubulose cutter hub.
4. biological sample asepticize sampling unit according to claim 2, is characterized in that: described Tubular tool side has multiple aperture near described beveled edge place.
5. biological sample asepticize sampling unit according to any one of claim 1 to 4, is characterized in that: described air supply unit comprises rubber suction bulb, air bag, air pump or gas blower.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410464751.9A CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410464751.9A CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
Publications (2)
| Publication Number | Publication Date |
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| CN104232477A true CN104232477A (en) | 2014-12-24 |
| CN104232477B CN104232477B (en) | 2016-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410464751.9A Active CN104232477B (en) | 2014-09-12 | 2014-09-12 | Biological sample asepticization sampler |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109475873A (en) * | 2016-06-16 | 2019-03-15 | 生物梅里埃公司 | For the end fitting and device of microbe colony sampling and the sampling method for using them |
| CN112048439A (en) * | 2020-07-27 | 2020-12-08 | 中国科学院广州能源研究所 | Sampling and culturing device for preventing disturbance of marine microorganism system for experiment |
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101057749A (en) * | 2006-04-17 | 2007-10-24 | 黄樟焱 | Novel clever seat with preventing secondary pollution function |
| CN102114390A (en) * | 2009-12-30 | 2011-07-06 | 中国科学院生态环境研究中心 | Reinforced type polyvinylidene fluoride hollow fiber hydrophobic membrane and preparation method thereof |
| CN102304471A (en) * | 2011-08-18 | 2012-01-04 | 深圳国际旅行卫生保健中心 | Liquid filtration-type air microbial sampler and sampling system |
| CN103908898A (en) * | 2013-01-07 | 2014-07-09 | 吕晓龙 | Composite hollow fiber film and making method thereof |
-
2014
- 2014-09-12 CN CN201410464751.9A patent/CN104232477B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101057749A (en) * | 2006-04-17 | 2007-10-24 | 黄樟焱 | Novel clever seat with preventing secondary pollution function |
| CN102114390A (en) * | 2009-12-30 | 2011-07-06 | 中国科学院生态环境研究中心 | Reinforced type polyvinylidene fluoride hollow fiber hydrophobic membrane and preparation method thereof |
| CN102304471A (en) * | 2011-08-18 | 2012-01-04 | 深圳国际旅行卫生保健中心 | Liquid filtration-type air microbial sampler and sampling system |
| CN103908898A (en) * | 2013-01-07 | 2014-07-09 | 吕晓龙 | Composite hollow fiber film and making method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 吕经烈: "中空纤维膜技术及其应用", 《海洋技术》, vol. 21, no. 4, 31 December 2002 (2002-12-31), pages 73 - 76 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109475873A (en) * | 2016-06-16 | 2019-03-15 | 生物梅里埃公司 | For the end fitting and device of microbe colony sampling and the sampling method for using them |
| CN112048439A (en) * | 2020-07-27 | 2020-12-08 | 中国科学院广州能源研究所 | Sampling and culturing device for preventing disturbance of marine microorganism system for experiment |
| CN112048439B (en) * | 2020-07-27 | 2022-05-17 | 中国科学院广州能源研究所 | Sampling and culturing device for preventing disturbance of marine microorganism system for experiment |
| CN114149900A (en) * | 2021-11-30 | 2022-03-08 | 中国科学院天津工业生物技术研究所 | Cutting head and hypha cutting method |
| CN114149900B (en) * | 2021-11-30 | 2024-03-29 | 中国科学院天津工业生物技术研究所 | Cutting head and mycelium cutting method |
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| Publication number | Publication date |
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| CN104232477B (en) | 2016-06-22 |
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