CN207016769U - A kind of oocyte and embryo's manipulation in vitro device - Google Patents
A kind of oocyte and embryo's manipulation in vitro device Download PDFInfo
- Publication number
- CN207016769U CN207016769U CN201720410247.XU CN201720410247U CN207016769U CN 207016769 U CN207016769 U CN 207016769U CN 201720410247 U CN201720410247 U CN 201720410247U CN 207016769 U CN207016769 U CN 207016769U
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- China
- Prior art keywords
- plastic straw
- embryo
- manipulation
- stiff
- thick
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 20
- 238000000338 in vitro Methods 0.000 title claims abstract description 18
- 210000000287 oocyte Anatomy 0.000 title claims abstract description 15
- 239000004033 plastic Substances 0.000 claims abstract description 32
- 229920003023 plastic Polymers 0.000 claims abstract description 32
- 239000010902 straw Substances 0.000 claims abstract description 25
- 239000011521 glass Substances 0.000 claims abstract description 15
- 239000012528 membrane Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 3
- 238000011109 contamination Methods 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 241001465754 Metazoa Species 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 4
- 108010000912 Egg Proteins Proteins 0.000 description 4
- 210000004681 ovum Anatomy 0.000 description 4
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000036318 Callitris preissii Species 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000010374 somatic cell nuclear transfer Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of oocyte and embryo's manipulation in vitro device, includes suction pipe, plastic straw one, filter, plastic straw two, biofilter, flexible rubber hose, plastics extra heavy pipe, thick and stiff sebific duct and the glass pipette being connected successively.Stopper can be set on the thick and stiff sebific duct.In use, operating personnel can fix suction pipe by face, and the plastic straw one and plastic straw two that are connected by small filter are enclosed on into neck, can mitigate long period of operation fatigue；One hand fixes glass pipette by thick and stiff sebific duct, can easily fix glass pipette；The utility model design science is rationally, operation is light, cost is low, sample collection is accurate, while can also prevent germ contamination, efficiently and safely can carry out manipulation in vitro to sample.
Description
Technical field
The utility model belongs to animal reproduction instrument field, and in particular to a kind of oocyte and embryo's manipulation in vitro dress
Put.
Background technology
Animal reproduction technology is the key technique in Animal husbandry production, numerous in high-quality, highly efficient and productive modern breeding stock
Educating in system has the function that to hold the balance.With the continuous development of domestic and international biotechnology, the research of animal reproduction new technology
Deepen continuously, it is using increasingly extensive.It is in vitro fertilization be after artificial insemination, embryo transfer technology, livestock reproduction field
Third time revolution；Somatic cell nuclear transfer technique is in the history of biology or even epoch-making breakthrough in whole life science.This
Two technologies have great importance to Animal husbandry production and protection animals on the brink of extinction etc..Pass through oocyte IVM
It is ripe and in vitro fertilization, a large amount of of fine quality, inexpensive embryo in vitro can be obtained, this breeding that can fully excavate excellent male and female poultry is dived
Power；The Population of improved seeds can efficiently be increased by ovocyte in-vitro and body-cell neucleus transplanting, be science
Research provides a large amount of inhereditary features consistent experimental animal.
At present, it is to be completed by common mouth suction pipe mostly, i.e., when gathering in the lab and shift oocyte or embryo
By in oocyte or embryo's suction inlet suction pipe, it is transferred in holding vessel.In practical operation, mouth suction pipe used is BJ-40
Thin glass tube（2.5×2.0×100）Due to relatively thin unmanageable, long-time operation is easily tired, and misoperation easily pollutes, and influences
Ovum and embryo quality etc..
Utility model content
Technical problem to be solved in the utility model is：There is provided one kind can be convenient to oocyte or embryo operation, accurate
Really, safety, quickly a kind of device, the present invention can effectively overcome the weak point of current ovum or embryo's manipulation in vitro.This
Utility model technical scheme：
A kind of oocyte and embryo's manipulation in vitro device, a kind of oocyte and embryo's manipulation in vitro device, according to
The secondary suction pipe, plastic straw one, filter, plastic straw two, biofilter, flexible rubber hose, plastics extra heavy pipe, thick and stiff including being connected
Sebific duct and glass pipette.Stopper can be set on the thick and stiff sebific duct.The biofilter uses 0.22 micron membrane filter.
Breather cheek valve is installed on plastic straw one, while breeches pipe is connected with plastic straw one, on the breeches pipe
Deflation check valve is installed, breeches pipe end set has deflating valve.The deflating valve contains that center is self-styled to remain silent, and center is self-styled to remain silent
Both ends on the outside of be provided with pressing plane.
Beneficial effect：In use, operating personnel can fix suction pipe by face, the modeling being connected by small filter
Material tubule one and plastic straw two are enclosed on neck, can mitigate long period of operation fatigue；One hand is fixed glass by thick and stiff sebific duct and inhaled
Pipe, can easily fix glass pipette；This glass pipette is that raw glass dropper draws form at high temperature, can autoclaving repeat to make
With；Two filters reach double protection simultaneously, prevent germ contamination.The utility model design science is reasonable, operation is light
Pine, cost is low, sample collection is accurate, while can also prevent germ contamination, efficiently and safely can carry out manipulation in vitro to sample.
Brief description of the drawings
Fig. 1 is one of structural relation schematic diagram of the present utility model；
Fig. 2 is the two of structural relation schematic diagram of the present utility model.
In figure, label 1 is suction pipe, and 2 be plastic straw one, and 3 be filter, and 4 be plastic straw two, and 5 be biofilter, 6
It is plastics extra heavy pipe for flexible rubber hose, 7,8 be rigid rubber pipe, and 9 be glass pipette, and 10 be branch pipe, and 11 be breather cheek valve, and 12 be deflation
Check valve, 13 be deflating valve.
Embodiment
Embodiment 1：Such as Fig. 1, a kind of oocyte and embryo's manipulation in vitro device, it includes suction pipe 1, plastic straw one
2, filter 3, plastic straw 24, biofilter 5, flexible rubber hose 6, plastic tube 7, thick and stiff sebific duct 8, glass pipette 9, the saturating suction pipe 1 of institute
It is placed on plastic straw 1；Plastic straw 1 is connected with plastic straw 24 by small filter；Small filter is mainly considered except in air
Impurity, the end of plastic straw 24 are connected with flexible rubber hose 6 by 0.22 micron membrane filter；Flexible rubber hose 6 and thick and stiff sebific duct 8 pass through
Plastics extra heavy pipe 7 is connected；Glass pipette 9 is connected with thick and stiff sebific duct 8.The plastics extra heavy pipe can use transparent material.
Using when, operating personnel's left hand is taken and includes suction pipe 1 and fixed with mouth, while plastic straw 2 and plastic straw 4 are passed through into neck
Portion is placed, and the right hand holds the thick and stiff sebific duct 8 of the present apparatus and fixes glass pipette 9, is then placed in glass pipette tip and has been put into embryo
In embryo's ware of tire or ovum, embryo in vitro or ovum are operated by the dynamics for sucking air.
Embodiment 2：Such as Fig. 2, on the basis of embodiment 1, and breather cheek valve is installed on plastic straw one, while
Breeches pipe is connected with plastic straw one, deflation check valve is installed on the breeches pipe, breeches pipe end set has deflating valve.The deflation
Mouth contains that center is self-styled to remain silent, and center is provided with pressing plane on the outside of the self-styled both ends remained silent.During nature, in deflating valve
The heart is self-styled to remain silent in closed state, after its end flat is pressed with left hand, can open deflating valve.
Claims (3)
1. a kind of oocyte and embryo's manipulation in vitro device, it is characterized in that：Include suction pipe, the plastic straw being connected successively
First, filter, plastic straw two, biofilter, flexible rubber hose, plastics extra heavy pipe, thick and stiff sebific duct and glass pipette；In plastic straw one
On breather cheek valve is installed, while be connected with breeches pipe on plastic straw one, deflation check valve, breeches pipe be installed on the breeches pipe
End set has deflating valve；The deflating valve contains that center is self-styled to remain silent, and center is provided with pressing on the outside of the self-styled both ends remained silent
Plane.
2. oocyte according to claim 1 and embryo's manipulation in vitro device, it is characterized in that：The biofilter
Using 0.22 micron membrane filter.
3. oocyte according to claim 1 and embryo's manipulation in vitro device, it is characterized in that：In the thick and stiff sebific duct
Upper setting stopper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720410247.XU CN207016769U (en) | 2017-04-19 | 2017-04-19 | A kind of oocyte and embryo's manipulation in vitro device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720410247.XU CN207016769U (en) | 2017-04-19 | 2017-04-19 | A kind of oocyte and embryo's manipulation in vitro device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207016769U true CN207016769U (en) | 2018-02-16 |
Family
ID=61460619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720410247.XU Expired - Fee Related CN207016769U (en) | 2017-04-19 | 2017-04-19 | A kind of oocyte and embryo's manipulation in vitro device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207016769U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
-
2017
- 2017-04-19 CN CN201720410247.XU patent/CN207016769U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110257229A (en) * | 2019-07-08 | 2019-09-20 | 河南省农业科学院畜牧兽医研究所 | It is a kind of simply to inhale fetus device and preparation method thereof |
| CN110257229B (en) * | 2019-07-08 | 2023-11-07 | 河南省农业科学院畜牧兽医研究所 | Simple embryo suction device and manufacturing method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180216 |