CN114190285B - Aseptic mouse preparation and integrated isolating device breeds - Google Patents
Aseptic mouse preparation and integrated isolating device breeds Download PDFInfo
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- CN114190285B CN114190285B CN202111434546.4A CN202111434546A CN114190285B CN 114190285 B CN114190285 B CN 114190285B CN 202111434546 A CN202111434546 A CN 202111434546A CN 114190285 B CN114190285 B CN 114190285B
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- 238000000034 method Methods 0.000 claims abstract description 23
- 238000002955 isolation Methods 0.000 claims abstract description 19
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Clinical Laboratory Science (AREA)
- Health & Medical Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Housing For Livestock And Birds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an integrated isolation device for sterile mouse preparation and breeding, which comprises: the mobile operation platform, placed stereoplasm transparent glass isolator on its top face, soft transparent isolator and stereomicroscope, the first stereoplasm glass cylinder opening on the stereoplasm transparent glass isolator and the connecting sleeve sealing connection on the soft transparent isolator, stereoplasm transparent glass isolator is arranged in between stereomicroscope's objective table and objective, be equipped with first gloves oversleeve and second gloves oversleeve on stereoplasm transparent glass isolator and the soft transparent isolator is close to the lateral wall of operator respectively, be equipped with first operation gloves and second operation gloves in first gloves oversleeve and the second gloves oversleeve respectively. This aseptic mouse preparation and integrated isolating device breeds aseptic mouse preparation and process of breeding unitedly combines, and not only use cost is low, the simple structure, the volume is less, convenient operation can show improvement aseptic mouse scale production preparation efficiency moreover.
Description
Technical Field
The invention relates to the technical field of sterile mouse preparation and breeding, in particular to an integrated isolation device for sterile mouse preparation and breeding.
Background
In recent years, as microbiology has rapidly developed, it is becoming clear that we have come to the form of "superlife" where each individual is a symbiont of the body with a large number of microorganisms. The microorganisms are mainly distributed in tissue parts or passages of the body, such as respiratory tract, digestive tract, urogenital tract, body surface and the like, which are directly communicated or contacted with the external environment; among them, the intestinal microorganisms are various and large in number, accounting for 78% of the total microbial population of the organism, and play a very important role in the pathological or physiological metabolic processes.
In view of the fact that intestinal microorganisms can be widely involved in various life metabolic activities of human beings and animals from life to death, the research on the composition structure and function of the intestinal microorganisms and the interaction relationship with a host has a very profound practical significance.
In order to carry out intensive studies on the intestinal microbiota, humans have developed a large number of sterile animal models. The sterile mice have the advantages of easiness in genetic engineering operation and humanization treatment, convenience in feeding and management, high growth speed, short breeding period, large-scale production and the like, and have good portability and reproducibility in combination with the intestinal microbiota, so that the sterile mice are widely applied to verification of correlation and causal relationship between phenotypes of human major diseases, animal husbandry economic traits and the like and the intestinal microorganisms, exploration of biological functions of different intestinal microorganisms and the like.
Aseptic preparation is a key link for obtaining aseptic mice of different strains or different genetic engineering and is divided into two methods, namely hysterectomy and embryo transplantation. Currently, hysterectomy is the mainstream method for preparing sterile mice, mainly due to the relatively low technical threshold of the method, and the theoretical basis and practical basis are that the placenta has a barrier effect on microorganisms.
Compared with the uterine resection method, the embryo transplantation method can well reduce the pollution risk, improve the milk leaving rate (99-100%), and is more convenient, quicker and more efficient in preparing the genetic engineering sterile mice. However, the technical requirements are high, the stereomicroscope and the isolator are required to be used in combination for preparing the sterile mouse by using the embryo transfer method, and the current combination methods mainly comprise three methods: one is to put the body microscope into a clean bench after fully sterilizing the body microscope by means of the clean bench, and connect the bench with an isolator. After completion of embryo transfer surgery in the clean bench, the pseudopregnant mice were transported back to the isolator for feeding and testing. However, the preparation method has larger pollution risk, complex operation and low efficiency; the second method is that an isolator (with the top made of transparent glass) made of hard materials is combined with an external stereomicroscope, so that the technical requirement of embryo transplantation for preparing a sterile mouse is met, however, the hard isolator has the defects of large size, corrosion resistance, high pollution risk and high manufacturing cost, is not suitable for being used in large-scale production, and in order to meet the working distance requirement of the stereomicroscope, a convex object stage is required to be arranged in the isolator, and the stereomicroscope is placed on the top end surface of the isolator, so that the working distance requirement between the object stage and an objective lens on the stereomicroscope can be met, although the stereomicroscope does not need to be sterilized, the isolator is internally provided with the convex object stage, so that an arm of an operator is required to keep the same height as the convex object stage for operation during operation, the arm of the operator is required to be operated for a long time, the arm of the operator is sour and painful, and the use feeling is poor; the third method is to transmit the sterilized integral microscope into the isolator for use, and has the difficulty that the integral microscope is difficult to meet the use requirement of not only completely sterilizing but also not damaging the parts of the integral microscope. Therefore, the current sterile mouse preparation method is difficult to find that the preparation efficiency is low and a special isolator is lacked, so that the aim of preparing the sterile mouse in large-scale production cannot be completely met, and the wide application and further development of the sterile mouse are limited.
Therefore, the technical personnel in the field need to solve the problem of how to provide an integrated isolation device for preparing and breeding a sterile mouse, which has the advantages of low use cost, simple structure, small volume and convenient operation, and can obviously improve the scale production and preparation efficiency of the sterile mouse.
Disclosure of Invention
In view of the above, the invention provides the sterile mouse preparation and breeding integrated isolation device which is low in use cost, simple in structure, small in size and convenient to operate and can obviously improve the mass production and preparation efficiency of sterile mice.
In order to achieve the purpose, the invention adopts the following technical scheme:
an integrated isolation device for sterile mouse preparation and breeding comprises:
the bottom of the mobile operation platform is provided with a filtering fan;
the hard transparent glass isolator is arranged on the top end face of the mobile operation platform, a first hard glass cylinder opening is connected to one side wall of the hard transparent glass isolator in a hot melting mode, two second hard glass cylinder openings which are arranged at intervals are connected to the side face, close to an operator, of the hard transparent glass isolator in a hot melting mode, a first glove sleeve is connected to each second hard glass cylinder opening, and a first operation glove is arranged in a cuff, far away from the hard transparent glass isolator, of each first glove sleeve;
the soft transparent isolator is arranged on the top end face of the mobile operation platform and located on one side of the hard transparent glass isolator, a connecting sleeve is connected to one side wall of the soft transparent isolator in a hot-pressing mode, the connecting sleeve is hermetically sleeved on the opening of the first hard glass cylinder, two second glove sleeves which are arranged at intervals are connected to the side face, close to an operator, of the soft transparent isolator in a hot-pressing mode, second operating gloves are arranged in cuffs, far away from the soft transparent isolator, of the second glove sleeves, an air inlet cylinder filter and an exhaust cylinder filter are connected to the soft transparent isolator, the air inlet cylinder filter is connected with the filter fan through a hose, and a circular transfer window is arranged on the side face, opposite to the second glove sleeves, of the soft transparent isolator;
the stereoscopic microscope is arranged on the top end face of the movable operating platform, and the hard transparent glass isolator is arranged between an objective and an objective of the stereoscopic microscope.
According to the technical scheme, compared with the prior art, the invention discloses and provides the sterile mouse preparation and breeding integrated isolation device, the device can simultaneously realize two processes of preparing the sterile mouse by an embryo transplantation method and breeding the sterile mouse on the whole, two independent operation units are formed on the local part and are not interfered with each other, and the hard isolator and the soft isolator are organically combined by means of the connecting sleeves. The hard transparent glass isolator and the mosaic body of the stereomicroscope guarantee that the embryo can be conveniently and quickly washed, enriched, subpackaged, transplanted and the like under the completely sterile condition, the mosaic design not only avoids pollution caused by the external environment, but also can clearly observe the embryo and facilitate the operation of the embryo, and the stereomicroscope is externally arranged without sterilization and disinfection treatment, so that the service life of the stereomicroscope can be obviously prolonged, and the use cost is fully reduced; and the soft isolator has larger space, thereby being convenient for the management processes of daily feeding, breeding and the like of the germ-free mice. The integrated isolation device for external use of the stereomicroscope and organic combination of hard-soft materials is a final product for highly organically integrating different functional modules, has the advantages of low use cost, simple structure, small volume, convenience in operation and the like, can be used for sterile mouse preparation, is particularly suitable for sterile mouse preparation in genetic engineering, can uniformly combine the sterile mouse preparation and breeding processes, and can remarkably improve the large-scale production and preparation efficiency of sterile mice.
Further, the hard transparent glass isolator is a cuboid, the length, the width and the height of the hard transparent glass isolator are 500, 220 and 180mm, the diameter of the opening of the first hard glass cylinder is 150mm, the length of the first hard glass cylinder is 120mm, the diameter of the opening of the second hard glass cylinder is 150mm, the length of the second hard glass cylinder is 80mm, and the working distance of the stereomicroscope is larger than 180mm and smaller than 286mm.
The beneficial effect that adopts above-mentioned technical scheme to produce is that, the stereoplasm transparent glass isolator is small, and not only occupation space is little, easily combines to use with the stereomicroscope moreover, has satisfied the clear technical requirement who is convenient for observe and operate of embryo's formation of image during preparation.
Furthermore, the sleeve sealing sleeve is arranged on the opening of the first hard glass cylinder, and an electrical adhesive tape and a transparent adhesive tape are wound on the sleeve sealing sleeve and the opening of the first hard glass cylinder.
The beneficial effect that adopts above-mentioned technical scheme to produce is, makes first stereoplasm glass cylinder opening and connecting sleeve connect fastening and sealed, guarantees the whole sterile environment that reaches through disinfecting after stereoplasm transparent glass isolator and the connection of soft transparent isolator.
Furthermore, the soft transparent isolator, the connecting sleeve, the first glove sleeve and the second glove sleeve are all made of polyvinyl chloride materials.
The beneficial effects that adopt above-mentioned technical scheme to produce are that, polyvinyl chloride material texture is soft, and intensity is higher, and long service life makes things convenient for the operator to develop aseptic mouse experiment and its chemical stability is good in the isolator, can be able to bear the erosion of acid-base and be favorable to electrician's sticky tape and scotch tape's sealed operation, guarantees integrated isolating device's sterile environment and maintains.
Furthermore, the first operating glove and the second operating glove are both nitrile rubber gloves.
The nitrile rubber gloves produced by adopting the technical scheme have the advantages of acid and alkali resistance, super-strong abrasion resistance and long service life, and are beneficial to ensuring the sterile environment maintenance of the integrated isolation device.
Furthermore, a glass culture dish is placed in the center of the bottom inside the hard transparent glass isolator, an LED lighting lamp holder is embedded on an objective table of the stereomicroscope, and the LED lighting lamp holder corresponds to the glass culture dish in position.
Adopt the beneficial effect that above-mentioned technical scheme produced to be, LED illumination lamp stand can improve the inside luminance of isolator, can assist the stereomicroscope to carry out clear observation and also do benefit to relevant operation to the embryo on the glass culture dish.
Furthermore, the hard transparent glass isolator is made of high-light-transmission glass.
The beneficial effect that adopts above-mentioned technical scheme to produce is that, the high printing opacity characteristic that the transparent isolator of stereoplasm has can satisfy the technical requirement that the embryo enlargies clear formation of image and be convenient for observe under the body type microscope.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a first view angle of an isolation device for preparing and breeding a sterile mouse according to the present invention.
Fig. 2 is a schematic structural diagram of a second view angle of the isolation device for integrated sterile mouse preparation and breeding provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the embodiment of the invention discloses an isolation device for preparing and breeding a sterile mouse, which comprises:
the filter device comprises a movable operating platform 1, wherein a filter fan 2 is arranged at the bottom of the movable operating platform 1;
the hard transparent glass isolator 3 is arranged on the top end face of the mobile operation table 1, a first hard glass cylindrical opening 31 is connected to one side wall of the hard transparent glass isolator 3 in a hot melting mode, two second hard glass cylindrical openings 32 which are arranged at intervals are connected to the side face, close to an operator, of the hard transparent glass isolator 3 in a hot melting mode, a first glove sleeve 4 is connected to each second hard glass cylindrical opening 32, and first operation gloves 5 are arranged in cuffs, far away from the hard transparent glass isolator 3, of each first glove sleeve 4;
the soft transparent isolator 6 is arranged on the top end face of the mobile operation table 1 and is positioned on one side of the hard transparent glass isolator 3, a connecting sleeve 7 is connected to one side wall of the soft transparent isolator 6 in a hot-pressing mode, the connecting sleeve 7 is sleeved on the opening 31 of the first hard glass cylinder in a sealing mode, two second glove sleeves 8 which are arranged at intervals are connected to the side face, close to an operator, of the soft transparent isolator 6 in a hot-pressing mode, a second operating glove 9 is arranged in a cuff, far away from the soft transparent isolator 6, of each second glove sleeve 8, an air inlet cylinder filter 10 and an exhaust cylinder filter 11 are connected to the soft transparent isolator 6, the air inlet cylinder filter 10 is connected with the filtering fan 2 through a hose 12, and a round transfer window 13 is arranged on the side face, opposite to the second glove sleeve 8, of the soft transparent isolator 6;
the stereomicroscope 14, the stereomicroscope 14 is disposed on the top end surface of the moving stage 1, and the hard transparent glass isolator 3 is disposed between the stage 141 and the objective lens 142 of the stereomicroscope 14.
The hard transparent glass isolator 3 is a cuboid, the length, width and height of the hard transparent glass isolator are 500, 220 and 180mm, the diameter of the first hard glass cylinder opening 31 is 150mm, the length of the first hard glass cylinder opening is 120mm, the diameter of the second hard glass cylinder opening 32 is 150mm, the length of the second hard glass cylinder opening is 80mm, and the working distance of the stereomicroscope 14 is larger than 180mm and smaller than 286mm.
The connecting sleeve 7 is hermetically sleeved on the first hard glass cylinder opening 31, and an electrical adhesive tape and a transparent adhesive tape are wound on the connecting sleeve and the first hard glass cylinder opening 31.
The soft transparent isolator 6, the connecting sleeve 7, the first glove sleeve 4 and the second glove sleeve 8 are all made of polyvinyl chloride materials.
The first and second operating gloves 5 and 9 are nitrile rubber gloves.
The center of the bottom inside the hard transparent glass isolator 3 is provided with a glass culture dish 15, an objective table 141 of the stereomicroscope 14 is embedded with an LED lighting lamp holder 16, and the LED lighting lamp holder 16 corresponds to the glass culture dish 15.
The hard transparent glass isolator 3 is made of high-light-transmission glass.
In the above scheme, after the first glove sleeve, the first operating glove, the second glove sleeve and the second operating glove are respectively connected to the hard transparent glass isolator and the soft transparent isolator, and the first hard glass cylinder opening and the connecting sleeve are connected, peracetic acid is required to be used for spray sterilization, so that the sterile environment of the device is ensured.
When the novel air bag is used, air blown out by the filtering fan is filtered by the air inlet cylinder filter and then enters the soft transparent isolator to be inflated, the soft transparent isolator becomes a bag body after being inflated, waste gas in the soft transparent isolator is exhausted from the air exhaust cylinder filter, and then sterile mice and food are placed in the novel air bag through the round transfer window.
The device can realize two processes of preparing the sterile mouse by an embryo transplantation method and breeding the sterile mouse simultaneously on the whole, two independent operation units are formed on the local part and are not interfered with each other, and the hard isolator and the soft isolator are organically combined by the connecting sleeve. The hard transparent glass isolator and the mosaic body of the stereomicroscope guarantee that the embryo can be conveniently and quickly washed, enriched, subpackaged, transplanted and the like under the completely sterile condition, the mosaic design not only avoids pollution caused by the external environment, but also can clearly observe the embryo and facilitate the operation of the embryo, and the stereomicroscope is externally arranged without sterilization and disinfection treatment, so that the service life of the stereomicroscope is obviously prolonged, and the use cost is fully reduced; the soft isolator has larger space, and is convenient for the management processes of the daily feeding, breeding and the like of the sterile mice. The integrated isolation device for external use of the stereomicroscope and organic combination of hard-soft materials is a final product for carrying out high organic integration on different functional modules, has the advantages of low use cost, simple structure, small volume, convenience in operation and the like, can be used for preparing sterile mice, is particularly suitable for preparing sterile mice in genetic engineering, can combine the preparation and breeding processes of the sterile mice in a unified manner, and can obviously improve the large-scale production and preparation efficiency of the sterile mice.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. An integrated isolation device for preparing and breeding a sterile mouse is characterized by comprising:
the filter device comprises a movable operation platform (1), wherein a filter fan (2) is arranged at the bottom of the movable operation platform (1);
the hard transparent glass isolator (3) is arranged on the top end face of the mobile operation table (1), a first hard glass cylinder opening (31) is connected to one side wall of the hard transparent glass isolator (3) in a hot melting mode, two second hard glass cylinder openings (32) which are arranged at intervals are connected to the side face, close to an operator, of the hard transparent glass isolator (3) in a hot melting mode, a first glove sleeve (4) is connected to each second hard glass cylinder opening (32), and a first operation glove (5) is arranged in a cuff, far away from the hard transparent glass isolator (3), of each first glove sleeve (4);
the flexible transparent isolator is characterized by comprising a flexible transparent isolator (6), the flexible transparent isolator (6) is arranged on the top end face of the mobile operation table (1) and is positioned on one side of the hard transparent glass isolator (3), a connecting sleeve (7) is connected to one side wall of the flexible transparent isolator (6) in a hot-pressing mode, the connecting sleeve (7) is hermetically arranged on the first hard glass cylindrical opening (31), two second glove sleeves (8) which are arranged at intervals are connected to the side face, close to an operator, of the flexible transparent isolator (6) in a hot-pressing mode, a second operating glove (9) is arranged in the cuff, far away from the flexible transparent isolator (6), of each second glove sleeve (8), an air inlet cylinder filter (10) and an air outlet cylinder filter (11) are connected to the flexible transparent isolator (6), the air inlet cylinder filter (10) is connected with the filtering fan (2) through a hose (12), and a circular transfer window (13) is arranged on the side face, opposite to the second glove sleeves, of the flexible transparent isolator (6);
a stereomicroscope (14), wherein the stereomicroscope (14) is arranged on the top end surface of the mobile operation table (1), and the hard transparent glass isolator (3) is arranged between an object stage (141) and an objective lens (142) of the stereomicroscope (14);
the hard transparent glass isolator (3) and the stereomicroscope (14) are embedded into a whole body, so that embryos can be conveniently and rapidly washed, enriched, subpackaged and transplanted under a completely sterile condition; the soft transparent isolator (6) is convenient for the routine breeding and management process of the sterile mice, the hard transparent glass isolator (3) and the soft transparent isolator (6) are combined into a whole, so that the sterile mice can be efficiently prepared by using an embryo transplantation method, the newly prepared sterile mice can be rapidly propagated, and the large-scale population can be conveniently and rapidly obtained;
the hard transparent glass separator (3) is a cuboid, and the length, width and height of the hard transparent glass separator are 500, 220 and 180mm.
2. The integrated isolation device for sterile mouse preparation and breeding as claimed in claim 1, wherein the first hard glass cylinder opening (31) has a diameter of 150mm and a length of 120mm, the second hard glass cylinder opening (32) has a diameter of 150mm and a length of 80mm, and the working distance of the stereomicroscope (14) is greater than 180mm and less than 286mm.
3. The integrated isolation device for sterile mouse preparation and breeding as claimed in claim 1, wherein the connecting sleeve (7) is hermetically sealed on the first hard glass cylinder opening (31) and both are wrapped with electrical tape and scotch tape.
4. The integrated isolation device for sterile mouse preparation and breeding according to claim 1, wherein the soft transparent isolator (6), the connecting sleeve (7), the first glove sleeve (4) and the second glove sleeve (8) are all made of polyvinyl chloride.
5. The integrated isolation device for sterile mouse preparation and breeding according to claim 1, wherein the first and second operating gloves (5, 9) are nitrile rubber gloves.
6. The integrated isolation device for sterile mouse preparation and breeding according to claim 1, wherein a glass culture dish (15) is placed at the center of the bottom inside the hard transparent glass isolator (3), an LED lighting lamp holder (16) is embedded on a stage (141) of the stereomicroscope (14), and the LED lighting lamp holder (16) corresponds to the glass culture dish (15).
7. The integrated isolation device for sterile mouse preparation and breeding according to any one of claims 1 to 6, wherein the hard transparent glass isolator (3) is made of high-light-transmittance glass.
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CN2824560Y (en) * | 2005-10-11 | 2006-10-11 | 中国医学科学院实验动物研究所 | Asepsis isolator |
CN105684920A (en) * | 2016-03-23 | 2016-06-22 | 山东新华医疗器械股份有限公司 | Breed conservation isolator for genetic engineering mice |
CN205653445U (en) * | 2016-04-24 | 2016-10-19 | 北华大学 | Can be to real -time microbiological incubator who surveys of microorganism |
CN209759466U (en) * | 2019-03-20 | 2019-12-10 | 陆珊珊 | Detection and identification device for microorganisms |
JP7425468B2 (en) * | 2020-01-07 | 2024-01-31 | 株式会社ジック | Animal surgery vinyl isolator |
CN111603264A (en) * | 2020-06-02 | 2020-09-01 | 河南大学 | Portable low-temperature sterile microsurgery operation box |
CN111759526B (en) * | 2020-07-16 | 2024-06-28 | 河北医科大学 | Inhalation type anesthesia experiment platform device |
CN112219729A (en) * | 2020-11-06 | 2021-01-15 | 苏州市冯氏实验动物设备有限公司 | Live pig breeds with aseptic isolating device of combination formula |
CN213892304U (en) * | 2020-12-29 | 2021-08-06 | 天津博裕力牧科技有限公司 | Multifunctional vehicle with clean environment for producing and transplanting bovine embryos |
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2021
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