CN111759526B - Inhalation type anesthesia experiment platform device - Google Patents

Inhalation type anesthesia experiment platform device Download PDF

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Publication number
CN111759526B
CN111759526B CN202010683888.9A CN202010683888A CN111759526B CN 111759526 B CN111759526 B CN 111759526B CN 202010683888 A CN202010683888 A CN 202010683888A CN 111759526 B CN111759526 B CN 111759526B
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biosafety cabinet
cabinet
anesthesia
biological safety
experimental
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CN202010683888.9A
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CN111759526A (en
Inventor
袁芳
王升
尉红肖
石硌
王昕�
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Hebei Medical University
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Hebei Medical University
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00Appliances for supporting or fettering animals for operative purposes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
    • A61D7/04Devices for anaesthetising animals by gases or vapours; Inhaling devices
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/16Disinfection, sterilisation or deodorisation of air using physical phenomena
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L9/00Disinfection, sterilisation or deodorisation of air
    • A61L9/16Disinfection, sterilisation or deodorisation of air using physical phenomena
    • A61L9/18Radiation
    • A61L9/20Ultraviolet radiation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D46/00Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D53/00Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B25HAND TOOLS; PORTABLE POWER-DRIVEN TOOLS; MANIPULATORS
    • B25JMANIPULATORS; CHAMBERS PROVIDED WITH MANIPULATION DEVICES
    • B25J21/00Chambers provided with manipulation devices
    • B25J21/02Glove-boxes, i.e. chambers in which manipulations are performed by the human hands in gloves built into the chamber walls; Gloves therefor
    • HELECTRICITY
    • H05ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR
    • H05KPRINTED CIRCUITS; CASINGS OR CONSTRUCTIONAL DETAILS OF ELECTRIC APPARATUS; MANUFACTURE OF ASSEMBLAGES OF ELECTRICAL COMPONENTS
    • H05K9/00Screening of apparatus or components against electric or magnetic fields
    • H05K9/0073Shielding materials
    • H05K9/0081Electromagnetic shielding materials, e.g. EMI, RFI shielding
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2209/00Aspects relating to disinfection, sterilisation or deodorisation of air
    • A61L2209/10Apparatus features
    • A61L2209/14Filtering means

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Robotics (AREA)
  • Mechanical Engineering (AREA)
  • Anesthesiology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Electromagnetism (AREA)
  • Animal Husbandry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Housing For Livestock And Birds (AREA)

Abstract

The invention relates to an inhalation type anesthesia experiment platform device, which belongs to medical experiment equipment and comprises a biosafety cabinet for animal experiments, wherein one side surface of the biosafety cabinet is a transparent observation plate, a turnover opening and closing taking and placing door is arranged on the side wall of the biosafety cabinet, an anesthesia system is arranged in the biosafety cabinet, an electromagnetic shielding film is arranged on the inner wall of the biosafety cabinet, a magnetic guide plate is arranged on a bottom plate of the biosafety cabinet, experimental equipment is adsorbed and fixed on the magnetic guide plate, symmetrical arm extending holes are arranged on the transparent observation plate, corresponding sealing sleeves are arranged at the arm extending holes, a microscope is arranged in the biosafety cabinet, an eyepiece of the microscope is positioned above the two arm extending holes, a latex film in sealing connection with the transparent observation plate is arranged around the eyepiece, a universal joint is also arranged on the side wall of the biosafety cabinet, and the universal joint is connected with experiment gas supply equipment.

Description

Inhalation type anesthesia experiment platform device
Technical Field
The invention belongs to the technical field of medical experimental equipment, relates to anesthesia experimental equipment, and in particular relates to an inhalation type anesthesia experimental platform device.
Background
In medical experiments, small animals such as rats, mice and rabbits are often used as experimental objects, the small animals need to be anesthetized before the experiments, two existing anesthesia methods are mainly adopted, one is injection anesthesia, and the other is inhalation anesthesia.
The injection anesthesia experiment object has serious side effects of respiratory and cardiovascular inhibition, the dosage needs to be strictly controlled, and animal death is easy to cause, but the method is simple and convenient, has more complications, high death rate and strong randomness, and the experiment result can not well simulate the physiological state and has larger influence on the experiment result; the inhalation type anesthesia mode has small influence on the physiological index and vital sign of the animal, the animal is closer to the physiological state, the experimental result is less influenced by anesthesia interference, and the inhalation type anesthesia method is applicable to various animals and various experiments, is an international conventional anesthesia method and is easy to be acknowledged by the international peer. However, inhalation type anesthesia has the problem of anesthetic gas diffusion, which is easy to harm the health of the experimenter. Meanwhile, part of experiments are performed in a sterile environment while inhalation anesthesia is used, and the open experimental environment cannot meet the sterile requirement and can also have the problem of anesthetic gas diffusion. The operation environment is sterile and clean, and the requirements of the current teaching and scientific research experiments can be met only by the operation platform with inhalation anesthesia and waste gas purification and recovery. For the above reasons, improvements to existing anesthesia experiments are urgently needed.
Disclosure of Invention
The invention overcomes the defects of the prior art, and provides the inhalation type anesthesia experiment platform device which forms a sterile and clean environment in operation, has inhalation type anesthesia and waste gas purification and recovery functions, and integrates the common instrument and equipment and a recording system for animal experiments. The device is a complete animal experiment operation platform, can meet the common experiment operation requirements, can ensure the animal anesthesia effect and reduce side effects and complications; the safety of experimenters can be protected, and the working environment is improved; can recycle the anesthetic waste gas, reduce environmental pollution; the sterile operation environment can be provided, and the experimental effect is improved; the microscopic operation can be completed, and the experimental accuracy is improved; can shield interference and record various experimental data.
The specific technical scheme of the invention is as follows:
The utility model provides a suction type anesthesia experiment platform device, the device includes the biosafety cabinet that is used for animal experiments, biosafety cabinet side is transparent observation board, the key point is, biosafety cabinet lateral wall on be provided with can overturn the switching get put the door, be provided with anesthesia system in the biosafety cabinet, anesthesia system includes volatilize jar and induction box, the induction box links to each other with volatilize jar's gas output, the induction box size is less than gets put the door, be provided with one deck electromagnetic shielding membrane on the biosafety cabinet inner wall, be provided with the magnetic conduction board on biosafety cabinet's the bottom plate, the magnetic conduction board up end is fixed with experimental facilities with the magnetic bracket, be provided with symmetrical arm access hole on the transparent observation board, arm access hole department installs corresponding sealed sleeve, be provided with the microscope that has the universal support in the biosafety cabinet, its eyepiece is located two arm access hole intermediate positions top, be provided with the emulsion membrane with transparent observation board sealing connection around the eyepiece, emulsion membrane thickness is 1.5-2.5mm, still be provided with a set of universal joint on the biosafety cabinet lateral wall, this set of joint links to each other with universal joint with the universal joint of volatilizing jar and is supplied with the experimental facilities outside.
The biological safety cabinet is connected with an exhaust gas recovery system, the exhaust gas recovery system comprises an exhaust pipeline, an exhaust fan and an exhaust gas treatment device, the exhaust fan is positioned in the exhaust pipeline, and the exhaust gas treatment device is positioned outside the biological safety cabinet and is communicated and connected with the inside of the biological safety cabinet through the exhaust pipeline.
The experimental equipment comprises a manual micromanipulator.
The top end inside the biosafety cabinet is provided with an ultraviolet lamp and an air filtering system.
The number of the universal joints is not less than three, and one of the universal joints is connected with the experimental gas supply equipment which is a breathing machine.
The beneficial effects of the invention are as follows: according to the invention, the anesthesia system is placed in the relatively sealed biosafety cabinet, and the biosafety cabinet forms an experiment environment without electromagnetic interference through the electromagnetic shielding film so as to carry out experiments requiring electrostatic shielding such as nerve discharge and the like, if aseptic experiments such as brain injection, nerve discharge and the like are carried out, an ultraviolet lamp and an air filtering system can be additionally arranged at the inner top end, and the relatively closed environment inside the biosafety cabinet is combined, so that an aseptic environment space can be built, conditions are provided for the aseptic experiments so as to reduce pollution to the operation field, reduce the infection rate and the death rate, reduce the influence of experiments and postoperative complications on animal death rate and experimental results, and in addition, the relatively closed experiment environment in the biosafety cabinet is prevented from being influenced by external matters.
The biological safety cabinet is additionally provided with an arm extending hole with a sealed oversleeve and an eyepiece connected to the biological safety cabinet through a latex film, and a magnetic conduction plate is additionally arranged in the biological safety cabinet for adsorbing and fixing experimental equipment, such as a microscope with a universal bracket and a manual micromanipulator.
Drawings
Fig. 1 is a schematic structural view of the present invention.
In the attached drawings, 1, a biosafety cabinet, 2, a transparent observation plate, 3, a taking and placing door, 4, an induction box, 5, an electromagnetic shielding film, 6, a magnetic conduction plate, 7, an arm extending hole, 8, a sealed sleeve, 9, a latex film, 10, a universal joint, 11, an exhaust pipeline, 12, an exhaust fan, 13, a waste gas treatment device, 14, a universal bracket, 15, a microscope, 16, an ultraviolet lamp, 17 and an eyepiece.
Detailed Description
The invention relates to an inhalation type anesthesia experiment platform device, which comprises a biosafety cabinet 1 for animal experiments, wherein one side surface of the biosafety cabinet is a transparent observation plate 2, a turnover opening and closing access door 3 is arranged on the side wall of the biosafety cabinet 1, an anesthesia system is arranged in the biosafety cabinet 1, the anesthesia system comprises a volatilization tank and an induction box 4, the induction box 4 is connected with the gas output end of the volatilization tank, the size of the induction box 4 is smaller than that of the access door 3, the access door 3 can be freely accessed, a layer of electromagnetic shielding film 5 is arranged on the inner wall of the biosafety cabinet 1, the electromagnetic influence of the outside can be isolated, a magnetic conduction plate 6 is arranged on the bottom plate of the biosafety cabinet 1, experimental equipment is fixed on the magnetic conduction plate by virtue of a magnetic support, symmetrical arm extending holes 7 are formed in the transparent observation plate 2, corresponding sealing sleeves 8 are arranged at the arm extending holes 7, microscopes 15 with universal brackets 14 are arranged in the biosafety cabinet 1, the eyepieces 17 are positioned above the middle positions of the two arm extending holes 7, latex films 9.5 are arranged around the eyepieces 17, the latex films are connected with the transparent observation plate 2 in a sealing mode, a universal joint 10 mm is arranged on the side wall of the biosafety cabinet, and one universal joint 10 mm is connected with the outer side wall of the biosafety cabinet, and the universal joint 10 is connected with one of the biosafety cabinet, and the universal joint 10 mm is connected with the universal joint 10.
The embodiment is shown in fig. 1, the improvement is based on the structure of the existing biosafety cabinet 1, the transparent observation plate 2 on the existing biosafety cabinet 1 can slide up and down to be opened or closed, the transparent observation plate 2 needs to be closed after being closed, the biosafety cabinet 1 forms a relatively closed space, the side wall of the biosafety cabinet 1 is provided with a picking and placing door 3 which can pick and place small animals and is opened and closed in a turning mode, the size of the picking and placing door 3 is 30cm, the induction box 4 can penetrate through the picking and placing door 3, the upper end of the picking and placing door 3 is connected with the side wall of the biosafety cabinet 1 through a rotating shaft, a torsion spring is arranged on the rotating shaft, the bottom of the picking and placing door 3 can be opened in an upward turning mode, the picking and placing door 3 can be automatically turned down to be closed under the action of the torsion spring, a sealing rubber gasket is arranged on the periphery of the picking and placing door 3, when the picking and placing door 3 is closed, the sealing rubber gasket is formed to be closed at the moment, the volatilizing tank of the anesthesia system volatilizes gas into the induction box 4, the small animals enter the induction box 4 and are anesthetized, and can be operated by an experiment staff.
The electromagnetic shielding film 5 is arranged on the inner wall of the biosafety cabinet 1 and is used for shielding the interference of external electromagnetic to the internal experiment of the biosafety cabinet 1, the magnetic conduction plate 6 is arranged on the bottom surface of the biosafety cabinet 1, the magnetic conduction plate 6 is used for fixing experimental equipment, the experimental equipment comprises a microscope with a universal bracket and a manual micromanipulator, the base of the universal bracket and the base of the manual micromanipulator are magnetic supports and can be adsorbed and fixed on the magnetic conduction plate 6, the microscope can adjust the position and the direction through the universal bracket, the manual micromanipulator can realize the micro-movement adjustment of the upper, lower, left, right, front and back of the motor, and the mode of adsorbing the magnetic supports on the magnetic conduction plate 6 can ensure the stability of the experimental equipment in the use process.
In order to facilitate replacement of air in the biosafety cabinet 1 and injection of various experimental gases, at least three universal connectors 10 are arranged on the side wall of the biosafety cabinet 1, experimental gas supply equipment is connected to the outside of all the universal connectors 10, one of the universal connectors is connected with a breathing machine at the outer end, a breathing cover is connected at the inner end, the breathing cover is used for providing conventional breathing gas for experimental animals, an anesthetic gas tank is connected at one of the outer ends, a volatilization tank is connected at the inner end, and the volatilization tank is positioned in the biosafety cabinet to adjust the density of the anesthetic gas.
The biological safety cabinet 1 top is connected with exhaust gas recovery system, and exhaust gas recovery system includes exhaust duct 11, exhaust fan 12 and exhaust gas treatment device 13, when the experiment is over the back or when the experiment needs to take a breath midway, is discharged exhaust gas to exhaust gas treatment device 13 along exhaust duct 11 through the rotation of exhaust fan 12, and exhaust gas treatment device 13 is with exhaust gas purification treatment or recycle.
In order to meet the requirements of aseptic experiments, the ultraviolet lamps 16 are arranged at the top end of the inside of the biosafety cabinet 1, the four ultraviolet lamps 16 are distributed in a rectangular shape, the inside of the biosafety cabinet 1 is respectively irradiated from different angles, sterilization of the inner space of the biosafety cabinet 1 is performed, in addition, an air filtering system is further arranged in the biosafety cabinet, the air in an experimental environment is subjected to real-time circulating filtration, the pollution of the ultraviolet lamps and the air filtering system to the environment is reduced by combining the purification effect of the ultraviolet lamps and the air filtering system on the environment, the animal mortality caused by complications after the experiment is reduced, and the influence on experimental results is reduced, and the biosafety cabinet can be continuously put back into the SPF animal room after the operation is finished.
When experimental operation is carried out, the two arms of an operator stretch into the sealed sleeve 8, the sealed sleeve 8 is made of rubber gloves, the opening part is in sealing connection with the arm stretching hole 7, the operator passes through the arm stretching hole 7 through the sealed sleeve 8 to enter the biosafety cabinet 1, the rubber gloves are good in elasticity, the two hands of the operator can reach all positions inside the biosafety cabinet 1 through the rubber gloves, the operator can carry out experiments through the combination of the ocular 17 and the microscope 15, the ocular 17 freely moves through the latex film 9, the latex film 9 achieves sealing effect, the latex film 9 is made of liquid ultra-soft latex materials, the thickness is selected to be 2mm, the proper stretching deformation performance and strength are achieved, the ocular 17 can freely move within a certain range, a rubber rolling curtain is arranged outside the latex film 9, the ocular 17 can be covered by the rubber rolling curtain when the rubber rolling curtain is not used, and the microscope 6 can assist the experimenter in completing microscopic experiments or operations.
According to the invention, the experiment platform is built through the biosafety cabinet with the electromagnetic shielding film arranged on the inner wall, the anesthesia system is positioned in the biosafety cabinet, a series of influences caused by leakage of anesthetic gas are avoided, inhalation anesthesia is carried out on the small animals under a closed environment, various physiological indexes of the small animals do not fluctuate and are relatively close to normal values, an operator cannot be influenced by the anesthetic gas, experiments are directly carried out in the closed environment outside the anesthesia system, even if the small animals wake up in the experiment process and cannot escape from the experiment range, anesthesia can be carried out again or the experiments can be carried out continuously, uncontrollable state caused by replacement of the anesthesia environment to another experiment environment is avoided, the operation of the experiment platform device is simplified, the transfer process between anesthesia and experiments is simplified, the anesthesia time and the anesthesia effect can be accurately controlled, and meanwhile, the experiment result has relatively high referential property is ensured.

Claims (2)

1. The utility model provides a suction type anesthesia experiment platform device, the device is including being used for biological safety cabinet (1) of animal experiment, and biological safety cabinet one side is transparent observation board (2), its characterized in that: the biological safety cabinet (1) is provided with a fetching and placing door (3) capable of being turned over and opened and closed on the side wall, an anesthesia system is arranged in the biological safety cabinet (1), the anesthesia system comprises a volatilization tank and an induction box (4), the induction box (4) is connected with the gas output end of the volatilization tank, and the size of the induction box (4) is smaller than that of the fetching and placing door (3); an electromagnetic shielding film (5) is arranged on the inner wall of the biosafety cabinet (1), a magnetic conduction plate (6) is arranged on the bottom plate of the biosafety cabinet (1), and experimental equipment is fixed on the upper end surface of the magnetic conduction plate (6) by virtue of a magnetic bracket; the transparent observation plate (2) is provided with symmetrical arm extending holes (7), the corresponding sealed sleeves (8) are arranged at the arm extending holes (7), a microscope (15) with universal brackets (14) is arranged in the biosafety cabinet (1), an eyepiece (17) of the microscope is positioned above the middle positions of the two arm extending holes (7), latex films (9) in sealing connection with the transparent observation plate (2) are arranged around the eyepiece (17), the thickness of the latex films (9) is 1.5-2.5mm, a group of universal joints (10) are further arranged on the side wall of the biosafety cabinet (1), the inner end of one of the universal joints (10) is connected with a volatilization tank, and experimental gas supply equipment is connected to the outside of the universal joints (10);
the experimental equipment also comprises a manual micromanipulator;
The biological safety cabinet (1) is connected with an exhaust gas recovery system, the exhaust gas recovery system comprises an exhaust pipeline (11), an exhaust fan (12) and an exhaust gas treatment device (13), the exhaust fan (12) is positioned in the exhaust pipeline (11), and the exhaust gas treatment device (13) is positioned outside the biological safety cabinet (1) and is communicated with the inside of the biological safety cabinet (1) through the exhaust pipeline (11);
an ultraviolet lamp (16) and an air filtering system are arranged at the top end inside the biosafety cabinet (1).
2. The inhalation anesthesia experiment platform device according to claim 1, characterized in that: the number of the universal joints (10) is not less than three, and one of the universal joints is connected with the experimental gas supply equipment which is a breathing machine.
CN202010683888.9A 2020-07-16 2020-07-16 Inhalation type anesthesia experiment platform device Active CN111759526B (en)

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Publication number Priority date Publication date Assignee Title
CN113384423A (en) * 2021-07-09 2021-09-14 中国人民解放军海军军医大学第一附属医院 Simple sterile closed nude mouse operation assembly
CN114190285B (en) * 2021-11-29 2023-03-31 华中农业大学 Aseptic mouse preparation and integrated isolating device breeds

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CN205095813U (en) * 2015-11-03 2016-03-23 河北蓝梦生物医药科技有限公司 Accuse warm nature biohazard safety equipment
CN205181525U (en) * 2015-11-27 2016-04-27 南阳医学高等专科学校 Animal experiment control box
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