CN2824560Y - Asepsis isolator - Google Patents
Asepsis isolator Download PDFInfo
- Publication number
- CN2824560Y CN2824560Y CN 200520114540 CN200520114540U CN2824560Y CN 2824560 Y CN2824560 Y CN 2824560Y CN 200520114540 CN200520114540 CN 200520114540 CN 200520114540 U CN200520114540 U CN 200520114540U CN 2824560 Y CN2824560 Y CN 2824560Y
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- CN
- China
- Prior art keywords
- germfree
- isolator
- air
- utility
- model
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004500 asepsis Methods 0.000 title 1
- 238000002955 isolation Methods 0.000 claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 238000010171 animal model Methods 0.000 claims description 9
- 238000009423 ventilation Methods 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 210000001503 joint Anatomy 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 44
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 4
- 239000004698 Polyethylene Substances 0.000 abstract description 3
- -1 polyethylene Polymers 0.000 abstract description 3
- 229920000573 polyethylene Polymers 0.000 abstract description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 230000000007 visual effect Effects 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 abstract 3
- 238000009395 breeding Methods 0.000 abstract 2
- 230000001488 breeding effect Effects 0.000 abstract 2
- 230000002349 favourable effect Effects 0.000 abstract 2
- 241000699670 Mus sp. Species 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000011160 research Methods 0.000 description 9
- 244000005700 microbiome Species 0.000 description 6
- 229910001220 stainless steel Inorganic materials 0.000 description 6
- 239000010935 stainless steel Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000003139 buffering effect Effects 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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Abstract
The utility model discloses a device for breeding germfree animals, particularly an isolator for breeding germfree mice, germfree rabbits and other small animals. The utility model is composed of an isolation chamber, a germfree transmission chamber, a front door of the transmission chamber, a rear door of the transmission chamber, a disinfection mouth, an air inlet filter, an air inlet pipeline, an air blast machine box, an air outlet filter, an air exhaust pipeline, a working lamp and a pair of operating gloves. The germfree isolator has favorable stopping property, germfree animals are completely isolated from microbes in nature, peroxyacetic acid disinfection liquid is sprayed to and disinfects the interior of the utility model, corrosiveness is small, and a sterilization method is simple and convenient; a body of the germfree isolator is made of germfree polyethylene membranes and has portability, practicality, low cost, favorable transparency, the body of the germfree isolator can be viewed at any time, various kinds of operation is carried out through the gloves, and the utility model has convenient operation; in a visual field, a user can work outside, the animals, or food, or other articles are conveyed into the isolator from outside in a germfree way, and interior germfree environment is not destroyed; germfree air inlet and outlet systems are installed in the utility model, moderate interior high air pressure is kept and the utility model favors air exchange.
Description
Technical field
The utility model relates to a kind of device of raising germfree animal, especially raises the isolator of small animals such as aseptic mouse and aseptic rabbit, belongs to field of biology.
Background technology
Animal is normally raised under the open system condition, all be subjected to nature microorganism and parasitic invasion and attack, generally all be with bacterium, virus and parasite, it is healthy that the animal appearance that has seems, in fact some diseases or microorganism is arranged and parasitic infection under cover all.Therefore experimentizing with common animal, susceptibility is poor, reactivity is inconsistent, and experimental result lacks repeatability.Improve zooperal accuracy, just must turn out the qualified animal of standard, just can make experimental result correctly reliable, reach and react homogeneity and repeatability to laboratory animal being controlled aspect heredity and two in the microorganism.
Along with the reach of science, have higher requirement to laboratory animal, how to eliminate of the interference of laboratory animal self-disease to experimental result, experimental results show that: except the newborn animal that caesarean birth obtained, raising in the aseptic isolation barrier of strictness, do not have other way can eliminate laboratory animal itself with the contagiosity illness.Germfree animal produces and is widely used in the every field of medical research on a large scale, obtained the conventional animal experiment the great achievement that can't find, enriched the research contents of germfree animal.
So-called germfree animal just is meant the microorganism and the parasitic animal that can not detect any work.From microbiological viewpoint, usually in the body of laboratory animal and externally have a parasite, also often have bacterium and virus in the body, but also all be difficult to get rid of some potential infectious disease.In addition, contain antibody in the serum of common laboratory animal.So carry out medical scientific with conventional animal, will there be various interference, experimental result is often imprecise.Use germfree animal to do experiment and just can overcome the existing shortcoming of common experiment, make experimental result correctly reliable.Therefore, in the high experiment of required precision, germfree animal, gnotobiotic animal and specific-pathogen free animal have just become desirable laboratory animal.Germfree animal must with the isolated fully aseptic isolator of nature microorganism in raise, along with carrying out of germfree animal research, the raising device of germfree animal also constantly improves.The Koster of Germany in 1915 is created germfree animal first and is cultivated isolator.The breadboard Reyniers of nineteen twenty-eight U.S. University of Notre Dame bacteriology has created the large-scale isolator of germfree animal of stainless steel.From then on carried out the system experimentation of germfree animal.Now there are 48 germfree animal research units in the U.S., only in 5 of NIH research institutes, just is respectively equipped with the germfree animal research department.Nineteen thirty Gustafsson has designed the second type isolator, and the raw material of whole isolated device is that stainless steel is made, cost height, inconvenient operation.In Japan, begin to cultivate germfree animal from nineteen forty-six, the main part of isolator is done with stainless steel in river, nineteen fifty-nine palace, and with little glass window and sensitive manipulator, makes the stainless steel isolator reach fairly perfect stage.But not only heavy cost of this isolator but also costliness can not large-scale popularization be used, thereby have also limited carrying out of germfree animal work.Be engaged in the germfree animal research work in existing 24 units of Japan, 9 germfree animal research units are only just arranged in Tokyo.The fifties, invented carry out the method for surface sterilizing with Peracetic acid after, but Peracetic acid corrosivity is stronger, bigger to the stainless steel and other metal materials influence, P.C.Trexler has created the plastic foil isolator in Indiana, United States University of Notre Dame Lobund research department later on, but price is higher, is difficult to import in batches, is not suitable for China's national situation.
Summary of the invention
In order to overcome the existing excessive heaviness of aseptic isolator volume or to involve great expense or the deficiency of inconvenient operation, the utility model provides a kind of safety economy, the sufficient aseptic isolator of sterilizing.
The technical scheme that its technical problem that solves the utility model adopts is: aseptic isolator is provided with three parts, first is an isolation ward, second portion is between buffering, third part is an air exchanging apparatus, the spray holes and the rubber gloves that is sealed on the plastic foil of a sterilization soup are arranged between accommodating, and inner various operations are undertaken by gloves.Gloves are movable, can change, and for prolonging the gloves useful life, one deck canvas layer is pasted in the gloves outside; All pass through to come in and go out between buffering in the hole that two openings and a spraying disinfection soup are arranged between buffering, animal, feed, drinking-water and various apparatus, two openings will be opened alternately, in case between air pollution accommodates; Air exchanging apparatus is made of the air high efficiency particulate air filter, and the gas high efficiency particulate air filter can guarantee the air purity of isolation ward, can stop air inversion again, with anti-pollution.Inner air pressure should be slightly higher than the outside, is beneficial to ventilation.
The beneficial effects of the utility model are,
1. good leak tightness, germfree animal and nature microorganism are isolated fully;
2. germfree isolator is made of plastic, and inner with thimerosal spraying disinfections such as Peracetic acid, less to aseptic isolator internal corrosion, sterilizing methods is also greatly easy;
3. make aseptic isolator main part and adopt the non-toxic polyethylene film, light practicality, required cost only is 1/tens of a stainless steel isolator;
4. the non-toxic polyethylene film transparency is good, and the space of isolator and content can be observed at any time;
5. the inner various operations of aseptic isolator are undertaken by gloves, and are easy to operate, are convenient to feed, can carry out work from the outside at any time under the visual field, do not destroy inner gnotobasis;
6. the sterile channel that interior external door is arranged, animal or food and other article sterilely are transported to isolator inside and go from the outside, and not by bad inner gnotobasis, as the same when sending;
7. aseptic turnover gas system is housed, keeps the hyperbar of inner appropriateness, be beneficial to ventilation.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is a front view of the present utility model.
Fig. 2 is an end view of the present utility model.
Among the figure: 1. isolation ward, 2. the storehouse front door is 3. transmitted in the sterile transfer storehouse, 4. transmits the storehouse rear door, 5. sterilization mouthful, 6. air intake high efficiency particulate air filter, 7. intake stack, 8. air feeder case, 9. air-out high efficiency particulate air filter, 10. exhaust duct, 11. portable lamps, 12. operation gloves.
Embodiment
In the drawings, experimental animal feeding is in isolation ward (1), and air enters isolation ward (1) through air feeder case (8), intake stack (7), air intake high efficiency particulate air filter (6), passes through air-out high efficiency particulate air filter (9), exhaust duct (10) discharge then.Air inflow can be passed through valve regulated, in order to keep malleation and the rate of ventilation in the isolation ward.
Required article in the isolator by disinfecting bucket (annex) and sterile transfer storehouse (2) butt joint, after transmitting storehouse (2) sterilization, are opened and are transmitted storehouse rear door (4), enter isolation ward (1).
The article that the indoor needs of isolator are taken out, reverse operating is closed then and transmitted storehouse front door (3), and is after sterilization mouthful (5) sterilization, stand-by.
The raising of animal and test whole process in the isolation ward is finished by operation gloves (12).
Claims (5)
1. an aseptic isolator by isolation ward, transmits the storehouse, the sterilization mouth, filter, intake stack, air feeder case, portable lamp, the operation gloves are formed, and it is characterized in that: experimental animal feeding is in isolation ward, and air enters isolation ward through air feeder case, discharges through filter.
2. a kind of aseptic isolator according to claim 1 is characterized in that: air enters isolation ward through air feeder case, intake stack, air intake high efficiency particulate air filter.
3. a kind of aseptic isolator according to claim 1 is characterized in that: air inflow is by valve regulated, in order to keep malleation and the rate of ventilation in the isolation ward.
4. a kind of aseptic isolator according to claim 1 is characterized in that: required article in the isolator by disinfecting bucket and the butt joint of sterile transfer storehouse, through transmitting the storehouse rear door, enter isolation ward.
5. a kind of aseptic isolator according to claim 1 is characterized in that: finish all operations in the isolation ward by the operation gloves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520114540 CN2824560Y (en) | 2005-10-11 | 2005-10-11 | Asepsis isolator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520114540 CN2824560Y (en) | 2005-10-11 | 2005-10-11 | Asepsis isolator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2824560Y true CN2824560Y (en) | 2006-10-11 |
Family
ID=37064738
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520114540 Expired - Fee Related CN2824560Y (en) | 2005-10-11 | 2005-10-11 | Asepsis isolator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2824560Y (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103499891A (en) * | 2007-12-11 | 2014-01-08 | 日东电工株式会社 | System for manufacturing optical display device |
| CN103826747A (en) * | 2011-07-29 | 2014-05-28 | 赛多利斯斯泰迪Fmt股份有限公司 | Container for the aseptic transfer of a biopharmaceutical product |
| CN105684920A (en) * | 2016-03-23 | 2016-06-22 | 山东新华医疗器械股份有限公司 | Breed conservation isolator for genetic engineering mice |
| CN106172042A (en) * | 2016-07-05 | 2016-12-07 | 苏州新区枫桥净化设备有限公司 | A kind of zoonosis isolation monitoring cage tool |
| CN108496831A (en) * | 2017-02-27 | 2018-09-07 | 苏州市苏杭科技器材有限公司 | Experimental animal operates apparatus sterile attachment device |
| CN110447547A (en) * | 2019-09-10 | 2019-11-15 | 赛业（苏州）生物科技有限公司 | Sterilized isolator |
| CN110663568A (en) * | 2019-11-07 | 2020-01-10 | 深圳市泓腾生物科技有限公司 | Sterile soft bag isolator |
| CN114190285A (en) * | 2021-11-29 | 2022-03-18 | 华中农业大学 | Aseptic mouse preparation and integrated isolating device breeds |
| CN114521508A (en) * | 2022-02-25 | 2022-05-24 | 哈尔滨学院 | Lactobacillus is to intestinal mucosa's protective capacities simulation experiment device |
-
2005
- 2005-10-11 CN CN 200520114540 patent/CN2824560Y/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103499891A (en) * | 2007-12-11 | 2014-01-08 | 日东电工株式会社 | System for manufacturing optical display device |
| US9138949B2 (en) | 2007-12-11 | 2015-09-22 | Nitto Denko Corporation | System for manufacturing optical display device |
| CN103499891B (en) * | 2007-12-11 | 2016-03-23 | 日东电工株式会社 | The manufacturing system of optical display |
| CN103826747A (en) * | 2011-07-29 | 2014-05-28 | 赛多利斯斯泰迪Fmt股份有限公司 | Container for the aseptic transfer of a biopharmaceutical product |
| CN103826747B (en) * | 2011-07-29 | 2015-09-02 | 赛多利斯斯泰迪Fmt股份有限公司 | For the container of the sterile transfer of biopharmaceutics product |
| CN105684920A (en) * | 2016-03-23 | 2016-06-22 | 山东新华医疗器械股份有限公司 | Breed conservation isolator for genetic engineering mice |
| CN106172042A (en) * | 2016-07-05 | 2016-12-07 | 苏州新区枫桥净化设备有限公司 | A kind of zoonosis isolation monitoring cage tool |
| CN108496831A (en) * | 2017-02-27 | 2018-09-07 | 苏州市苏杭科技器材有限公司 | Experimental animal operates apparatus sterile attachment device |
| CN110447547A (en) * | 2019-09-10 | 2019-11-15 | 赛业（苏州）生物科技有限公司 | Sterilized isolator |
| CN110663568A (en) * | 2019-11-07 | 2020-01-10 | 深圳市泓腾生物科技有限公司 | Sterile soft bag isolator |
| CN110663568B (en) * | 2019-11-07 | 2024-04-26 | 深圳市泓腾生物科技有限公司 | Sterile soft package isolator |
| CN114190285A (en) * | 2021-11-29 | 2022-03-18 | 华中农业大学 | Aseptic mouse preparation and integrated isolating device breeds |
| CN114521508A (en) * | 2022-02-25 | 2022-05-24 | 哈尔滨学院 | Lactobacillus is to intestinal mucosa's protective capacities simulation experiment device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |