CN214881586U - Cell in-vitro culture system - Google Patents

Abstract

The utility model discloses an in vitro cell culture system, which comprises a first transfer window and an incubator. One side of the incubator is hinged with an incubator door with a reflector, one side of the first transfer window is provided with a first transfer window door, the other side of the first transfer window is provided with an opening, and the opening is connected with the incubator through a sealing component so that the incubator door is opened to the inside of the first transfer window; operating gloves are arranged on two side faces of the first transmission window; the first transmission window is internally provided with a sterilizing device and a set of clean air exhaust system. The utility model realizes the butt sealing of the incubator and the transmission window through the sealing strip; sterilizing the external surfaces of the transfer window and the internal objects thereof by built-in high-temperature sterilization equipment and VHP sterilization equipment; by using a dust-free cell culture bottle/bag/dish sealing device which is matched with a specific incubator and has the electronic identification bar code consistent with the specific incubator, the cell preparation can be accurately and safely transported in environments with different clean levels, and the cell preparation box is particularly suitable for cell cluster culture of different individual sources in the same cell preparation room.

Description

Cell in-vitro culture system

Technical Field

The utility model relates to a cell culture technical field especially relates to an external culture system of cell.

Background

The cell therapy comprises: individual or allogeneic cells are extracted, genetically or chemically modified in vitro, expanded into a large number of cells, and reintroduced into the individual for therapeutic purposes. The current best form of cell therapy is CAR-T therapy, in which an individual's immune cells are extracted, genetically modified to enable them to attack cancer cells, and reintroduced into the patient. Recently, this therapy has been recommended for FDA approval. However, cell therapy is not limited to immune cells, but the use of tissue progenitor cells to regenerate and repair tissue is also contemplated.

The most critical of cell therapy is cell culture, which requires a long period, and during the culture process, the cells need to be continuously replaced and supplemented with culture solution and various factors, which all need to be operated aseptically. In the research process of cell therapy and the like, the occurrence of contamination is one of the important factors of experimental failure. Once contamination has occurred, it means that the test needs to be restarted, wasting much time and economic cost. With the development of bioscience, the requirements on the sterility of a cell culture environment and the safety of people are more and more strict, and higher requirements are also put on a biological equipment.

In order to avoid pollution, a cell culture box, a biological safety cabinet centrifuge and a microscope are arranged in one GMP cell culture room at present, and in order to avoid cross contamination, only the same cell preparation is arranged in the same culture box, so that the same cell culture room can only be used for culturing cells of the same individual, and the space utilization rate and the production efficiency are both extremely low. For solving this problem the utility model aims at providing an external culture system of cell can place many in same GMP cell culture room culture system can satisfy same laboratory and operate different individual cells in the different times, can not have the pollution between the different individual cell, improves cell production efficiency.

SUMMERY OF THE UTILITY MODEL

The utility model provides a not enough to prior art, the utility model provides an external culture system of cell adopts this culture system can guarantee immediately after the cell goes out the incubator at the pass-through box internal seal, can be at the safe conversion of A level environment and non-A level environment, and the conversion process is pollution-free, has also improved cell production efficiency simultaneously.

In order to solve the technical problem, the technical scheme of the utility model as follows: a cell in-vitro culture system comprises a first transfer window and an incubator, wherein one side of the incubator is hinged with an incubator door, one side of the first transfer window is provided with a first transfer window door, the other side of the first transfer window is provided with an opening, the opening is connected with the incubator through a sealing component so that the incubator door can be opened to the inside of the first transfer window, and the size of the first transfer window is larger than that of the incubator;

at least one first operating glove is fixedly arranged on the side surface of the first transmission window; the first transfer window is also provided with a sterilization device for sterilizing the interior of the first transfer window.

Preferably, the sterilization device comprises a high-temperature sterilization device and/or a VHP sterilization device, and the high-temperature sterilization device comprises a high-temperature steam pipeline, a temperature sensing probe and a controller which are arranged on the first transmission window;

VHP sterilizing installation's gas outlet fixedly connected with trachea, the trachea is through first flange fixed mounting in top one side of first pass-through box, the trachea is linked together with first pass-through box, there is the back flow one side bottom of first pass-through box through second flange fixed mounting, the other end and VHP sterilizing installation's air inlet fixed connection of back flow.

Preferably, the sealing assembly comprises a rectangular groove arranged on the peripheral inner wall of the opening, an inflatable sealing strip is fixedly mounted on the inner wall of the rectangular groove, the inner side of the inflatable sealing strip is abutted to the outer side of the incubator, a pipeline is fixedly mounted on one side of the inflatable sealing strip, an electromagnetic valve is fixedly mounted on the pipeline, and the pipeline is connected with an air source of compressed air.

Preferably, a top transparent window is fixedly mounted on the top of the first transmission window, and the front surface and the side surface of the first transmission window are in a transparent state.

Preferably, adjustable feet are fixedly arranged at the rear positions of the bottom of the incubator.

Preferably, a baffle is fixedly installed on the inner wall of the bottom of the first transmission window, and one side of the baffle is in contact with one side of the incubator.

Preferably, the first transfer window is further provided with a clean air exhaust system, a clean air inlet is arranged above the first transfer window, and a clean air outlet is arranged below the first transfer window.

Preferably, a second transfer window is fixedly installed on one side, away from the first operating glove, of the first transfer window in a sealing manner, second transfer window doors are hinged to two sides of the second transfer window, and the second transfer window door located on one side of the first transfer window is opened towards the interior of the first transfer window; and a second operating glove is fixedly arranged on one side of the first transmission window close to the second transmission window.

Preferably, a supporting plate is fixedly installed on one side of the opening of the first transmission window, a screw rod is connected to the top of the supporting plate in a penetrating mode through threads, a rubber plate is connected to the bottom of the screw rod in a rotating mode, the bottom of the rubber plate is abutted to the top of the incubator, and a handle is fixedly installed on the top of the screw rod.

Preferably, a sterilization device and a clean air exhaust system are arranged in the second transfer window.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. in the utility model, the incubator is placed in the first transmission window through the opening, the incubator is pushed towards the opening direction, the incubator is pushed into the first transmission window, when one side of the incubator is abutted to the baffle, the handle is rotated, the screw rod can be driven by the handle to rotate, the screw rod can rotate and descend on the support plate, the screw rod can drive the rubber plate to move downwards, the rubber plate can be contacted with the top of the incubator, and the abutting incubator fastened by the rubber plate can be fixed under the action of the screw rod;

2. in the utility model, the level of the incubator is adjusted by adjusting the adjustable feet, and by opening the electromagnetic valve, the compressed air can be inflated to the inflatable sealing strip through the pipeline, and the inflatable sealing strip can be expanded and is abutted against the outer side of the incubator to realize sealing;

3. in the utility model, before the incubator door is opened, the VHP sterilizing equipment is started, the VHP sterilizing equipment can convey hydrogen peroxide gas into the first transmission window from the upper part of the first transmission window, the first transmission window can be sterilized, the gas can flow back into the VHP sterilizing equipment through the return pipe, the VHP sterilizing equipment can detect whether sterilization is finished through the returned gas, after the sterilization of the first transmission window is finished, the incubator door is opened, the culture dish in the incubator is taken out and closed, the culture dish is sealed through the first operating glove, after the sealing operation is finished, the sealed culture dish is sterilized by hydrogen peroxide through the VHP sterilizing equipment again, after the sterilization is finished, the first transmission window door is opened, namely, the sterilized culture dish can be taken out from the first transmission window, in addition, after the hydrogen peroxide sterilization of the sealed culture dish is finished through the VHP sterilizing equipment, the second transfer window door located on one side of the first transfer window can be opened, the sterilized culture dish is placed on one side close to the second transfer window through the first operation gloves, then the second operation gloves are used for moving the sterilized culture dish into the second transfer window, the second transfer window door located on one side of the first transfer window is closed, the second transfer window door located on one side far away from the first transfer window is opened finally, the sterilized culture dish can be taken out, and then the culture box can be further prevented from being polluted by the outside.

The utility model realizes the butt sealing of the incubator and the transmission window through the inflatable sealing strip, and is convenient to disassemble; the external surfaces of the delivery window and the internal objects are thoroughly sterilized through high-temperature sterilization and VHP sterilization equipment arranged in the delivery window; through the supporting dustless cell culture bottle/bag/ware sealing device who is unanimous with specific incubator electronic identification bar code of cooperation use, realize accurate safe transportation in the environment of different clean grades of cell preparation, especially be fit for the cell cluster culture of the indoor different individual sources of same cell preparation.

Drawings

FIG. 1 is a schematic sectional view of a cell in vitro culture system according to the present invention;

FIG. 2 is a left side view of the in vitro cell culture system of the present invention;

FIG. 3 is a right side view of a first transmission window of an in vitro cell culture system according to the present invention;

FIG. 4 is a top view of a first transmission window of an in vitro cell culture system according to the present invention;

FIG. 5 is an enlarged view of part A of an in vitro cell culture system according to the present invention;

FIG. 6 is a schematic view of the inflatable sealing strip of the in vitro cell culture system of the present invention;

FIG. 7 is a schematic top view of a first transmission window of a cell in vitro culture system according to a second embodiment of the present invention;

FIG. 8 is a schematic top view of a first transmission window of a cell in vitro culture system according to a third embodiment of the present invention;

FIG. 9 is a schematic cross-sectional front view of a first transfer window of a cell in vitro culture system according to a third embodiment of the present invention;

FIG. 10 is an enlarged view of part B of a cell in vitro culture system according to a third embodiment of the present invention.

The labels in the figure are: 1. a first transmission window; 2. an incubator; 3. a VHP sterilization facility; 4. an air tube; 5. a first flange; 6. a first operating glove; 7. a first transfer window door; 8. a door opening; 9. a return pipe; 10. a second flange; 11. a top transparent window; 12. the ground feet can be adjusted; 13. the incubator door; 14. a second pass-through window door; 15. a support plate; 16. an opening; 17. an electromagnetic valve; 18. an inflatable sealing strip; 19. a pipeline; 20. a handle; 21. a screw; 22. a rectangular groove; 23. a rubber plate; 24. a baffle plate; 25. a second pass-through window; 26. a second operating glove; 27. a high temperature steam line; 28. a clean exhaust system; 29. an exhaust duct; 30. an air intake duct; 31. a controller; 32. a temperature sensing probe.

Detailed Description

The technical solutions of the present invention will be described in further detail with reference to the accompanying drawings and specific embodiments, but the present invention is not limited to the following technical solutions.

Example 1

Referring to fig. 1-6, an in vitro cell culture system, including first pass-through box 1 and incubator 2, the inside corner of first pass-through box 1 is the fillet, do benefit to the disinfection, one side of incubator 2 articulates there is incubator door 13, one side of first pass-through box 1 is equipped with first pass-through box door 7, the position of first pass-through box 1 installation pass-through box door in this embodiment is equipped with door opening 8, the size of first pass-through box door 7 is greater than the size of door opening 8, and can seal between first pass-through box door and the door opening, first pass-through box 1 opposite side is equipped with opening 16, thereby opening and incubator 2 pass through seal assembly and are connected and make incubator door 13 open to first pass-through box 1 is inside, the size of first pass-through box 1 is greater than the size of incubator 2, can hold the incubator door and open and place the cell culture bottle.

The first transfer window 1 is also provided with a clean air exhaust system 28, a clean air inlet is arranged above the first transfer window 1, a clean air outlet is arranged below the first transfer window 1, and a filtering membrane is arranged at the air inlet 3; the air return inlet and the air outlet of the clean air exhaust system 28 are respectively connected with an exhaust pipeline 29 and an air inlet pipeline 30, the exhaust pipeline 29 is connected with a clean air exhaust port, and the air inlet pipeline 30 is connected with a clean air inlet.

The sealing assembly comprises a rectangular groove 22 arranged on the inner wall of the opening 16, an inflatable sealing strip 18 is fixedly mounted on the inner wall of the rectangular groove 22, the inner side of the inflatable sealing strip 18 is abutted to the outer side of the incubator 2, a pipeline 19 is fixedly mounted on one side of the inflatable sealing strip 18, an electromagnetic valve 17 is fixedly mounted on the pipeline 19, the pipeline 19 is connected with an air source of compressed air, and sealing is carried out by inflating the sealing strip.

The top of the first transmission window 1 is fixedly provided with a top transparent window 11, and the front surface and the side surface of the first transmission window 1 are in a transparent state. The bottom of the rear part of the incubator 2 is fixedly provided with adjustable feet 12, so that the incubator can be adjusted to be balanced conveniently.

In the embodiment, two first operating gloves 6 are fixedly arranged on one side surface of the first transfer window 1, and the first operating gloves close to the incubator are 10-15cm away from the incubator door.

The first transfer window 1 is also provided with a sterilization device for sterilizing the inside of the first transfer window 1. Sterilizing equipment includes high temperature sterilization equipment and VHP sterilizing installation 3, high temperature sterilization equipment is including establishing the high temperature steam conduit 27 on first transmission window 1, fixed mounting has the solenoid valve on the high temperature steam conduit 27, high temperature steam conduit 27 is connected with high temperature steam production equipment, VHP sterilizing installation 3's gas outlet fixedly connected with trachea 4, trachea 4 is through first flange 5 fixed mounting in top one side of first transmission window 1, and trachea 4 is linked together with first transmission window 1, and one side bottom of first transmission window 1 has back flow 9 through second flange 10 fixed mounting, the other end of back flow 9 and VHP sterilizing installation 3's air inlet fixed connection. It should be noted that the positions of the air inlet and the air outlet can be adjusted according to actual conditions, the bottom of the first transfer window 1 is fixedly provided with the temperature sensing probe 32, one side of the first transfer window 1 is fixedly provided with the controller 31, the temperature sensing probe 32 is electrically connected with the electromagnetic valve and the controller 31, when high-temperature sterilization is required, the electromagnetic valve is started through the controller 31, the electromagnetic valve is opened, high-temperature steam can be conveyed into the first transfer window 1 through the high-temperature steam pipeline 27, the inside of the first transfer window 1 is sterilized at high temperature, and the temperature sensing probe 32 can monitor the temperature inside the transfer window. Thus, hydrogen peroxide can be used for sterilization in the test process, and high-temperature steam can be used for thorough sterilization after the test is finished.

CO selected for use in this example₂The cell culture box has the air sterilization and filtration function, the inside of the cell culture box adopts a hydrogen peroxide rapid sterilization mode for sterilization, and the cleanness grade is A grade.

The baffle 24 is fixedly arranged on the inner wall of the bottom of the first transfer window 1, and one side of the baffle 24 is in contact with one side of the incubator 2 for limiting.

CO₂The dimensions of the cell incubator are (length × width × height mm): 400-800-400-700-500-900, the size of 500-800 is selected for this embodiment.

The dimensions of the first transfer window are (length × width × height mm): in the embodiment of 600-1000 × 500-800 × 600-1000, the size of 900 × 650 × 850 is selected.

The dimensions of the second transmission window are (length × width × height mm): 400- > 1000- > 400- > 800- > 400- > 1000, the size of 500 × 500 × 500 is selected for the embodiment.

Example 2

Referring to fig. 7, the present embodiment is modified based on embodiment 1: the sealed fixed mounting in one side that first operation gloves 6 were kept away from to first pass-through box 1 has second pass-through box 25, and the both sides of second pass-through box 25 all articulate there is second pass-through box door 14, and second pass-through box door 14 that is located first pass-through box 1 one side is opened to first pass-through box 1 is inside, and the aim at of design like this prevents to open when first pass-through box door, and the outside air has the risk of getting into the incubator, prevents to have gaseous exchange between the incubator. The first transfer window 2 is provided with one or two second handling gloves 26 on opposite sides of the first handling glove. The second operating glove 26 is arranged to facilitate opening of the second transfer window door and opening or closing of the incubator, and a sterilization device and a clean air exhaust system are also arranged in the second transfer window.

After the sealed culture dish is sterilized by using the VHP sterilization apparatus 3 with hydrogen peroxide, the second transfer window door 14 located on one side of the first transfer window 1 may also be opened, the sterilized culture dish is placed on one side close to the second transfer window 25 by using the first operating gloves 6, then the sterilized culture dish is moved into the second transfer window 25 by using the second operating gloves 26, then the second transfer window door 14 located on one side of the first transfer window 1 is closed, and finally the second transfer window door 14 located on one side far from the first transfer window 1 is opened, so that the sterilized culture dish can be taken out, and the contamination of the incubator 2 with the outside can be further prevented.

Example 3

Referring to fig. 8 to 10, the present embodiment is modified based on embodiment 1: 1 opening one side fixed mounting of first pass-through window has backup pad 15, the top through thread connection of backup pad 15 has screw rod 21, the bottom of screw rod 21 is rotated and is connected with rubber slab 23, the bottom of rubber slab 23 is inconsistent with the top of incubator 2, the top fixed mounting of screw rod 21 has handle 20, twist grip 20, handle 20 can drive screw rod 21 and rotate, screw rod 21 can rotate the limit decline in backup pad 15, screw rod 21 can drive rubber slab 23 downstream, rubber slab 23 can contact with the top of incubator 2, under the effect of screw rod 21, can make the conflict incubator 2 of rubber slab 23 fastening, then can fix incubator 2, can prevent to take place relative displacement between incubator 2 and the first pass-through window 1.

The utility model discloses a work flow is: when the incubator is used, the pipeline 19 is firstly connected with a clean air source of compressed air, or is directly connected with an air compressor, the incubator 2 is placed in the first transfer window 1 through the opening 16, the incubator 2 is pushed towards the opening 16, the incubator 2 is pushed into the first transfer window 1, when one side of the incubator 2 is abutted to the baffle 24, the handle 20 is rotated, the handle 20 can drive the screw 21 to rotate, the screw 21 can rotate and descend on the support plate 15, the screw 21 can drive the rubber plate 23 to move downwards, the rubber plate 23 can be contacted with the top of the incubator 2, under the action of the screw 21, the rubber plate 23 can be tightly abutted to the incubator 2, the incubator 2 can be fixed, at the moment, the electromagnetic valve 17 is opened, the compressed air can inflate the inflatable sealing strip 18 through the pipeline 19, the inflatable sealing strip 18 can expand and is abutted to the outer side of the incubator 2, and sealing is realized.

Before the incubator door 13 is opened, the VHP sterilization device 3 is started, the VHP sterilization device 3 is a technology for generating free-OH for attacking cell components including lipids, proteins and DNA by using the advantage that hydrogen peroxide has more sporicidal capability in a gas state than in a liquid state at normal temperature, and meeting the requirement of complete sterilization, and the VHP sterilization device 3 can deliver gaseous hydrogen peroxide into the first delivery window 1 from above the first delivery window 1, so that the first delivery window 1 can be sterilized, the gas can flow back into the VHP sterilization device 3 through the return pipe 9, and the VHP sterilization device 3 can detect whether sterilization is completed through the returned gas; after the sterilization is finished, residual hydrogen peroxide in the transfer window can be taken away through the clean exhaust system.

After the first transmission window 1 is sterilized, the incubator door 13 is opened, the incubator in the incubator 2 is taken out, the incubator door 13 is closed, the incubator is packaged and sealed by the packaging bag through the first operating gloves 6, and the electronic identification bar codes which are consistent with those of the specific incubator are arranged on the incubator/dish and the sealing bag.

After the packaging operation is finished, the sealed culture bottle can be sterilized by hydrogen peroxide by the VHP sterilizing equipment 3, and after the sterilization is finished, the first transmission window door 7 is opened, so that the sterilized culture dish can be taken out from the first transmission window 1.

In order to prevent the indoor class B environment from affecting the environment inside the incubator or the first transfer window, it is preferable that the second transfer window door 14 located at one side of the first transfer window 1 is opened, the sterilized culture dish is placed at one side close to the second transfer window 25 by the first operating glove 6, the sterilized culture dish is moved into the second transfer window 25 by the second operating glove 26, the second transfer window door 14 located at one side of the first transfer window 1 is closed, and the second transfer window door 14 located at one side away from the first transfer window 1 is opened, and the sterilized culture dish can be taken out, so that the incubator 2 can be further prevented from being contaminated with the outside. The culture system isolates the risk of cross contamination of samples in different batches, improves the space utilization rate and the production efficiency, reduces the investment cost and the operation cost of the whole plant facility, and is provided with valves (not shown in the figure) on each gas pipeline. After the test is finished, high-temperature sterilization equipment can be selected to be started for thorough sterilization.

Of course, as is well known to those skilled in the art, the VHP sterilization apparatus 3, the clean air exhaust system 28, the controller 31, the temperature sensing probe 32 and the electromagnetic valve are all conventional means or common knowledge, and the working principle and the wiring manner thereof are also common knowledge of those skilled in the art, and will not be described herein again, and those skilled in the art can make any choice according to their needs or convenience. The materials of the components involved in the present embodiment, such as the transmission window, the operation gloves, etc., are required to be resistant to high temperature.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (10)

1. The in vitro cell culture system is characterized by comprising a first transfer window (1) and an incubator (2), wherein one side of the incubator (2) is hinged with an incubator door (13), one side of the first transfer window (1) is provided with a first transfer window door (7), the other side of the first transfer window (1) is provided with an opening (16), the opening is connected with the incubator (2) through a sealing component, so that the incubator door (13) is opened towards the inside of the first transfer window (1), and the size of the first transfer window (1) is larger than that of the incubator (2);

at least one first operating glove (6) is fixedly arranged on the side surface of the first transmission window (1); the first delivery window (1) is also provided with a sterilization device for sterilizing the interior of the first delivery window (1).

2. The system for in vitro culturing of cells according to claim 1, wherein the sterilization device comprises a high temperature sterilization device and/or a VHP sterilization device (3), the high temperature sterilization device comprises a high temperature steam pipeline (27) arranged on the first transmission window (1), a temperature sensing probe and a controller;

the gas outlet fixedly connected with trachea (4) of VHP sterilizing installation (3), trachea (4) are through first flange (5) fixed mounting in top one side of first transmission window (1), trachea (4) are linked together with first transmission window (1), there is back flow (9) one side bottom of first transmission window (1) through second flange (10) fixed mounting, the other end of back flow (9) and the air inlet fixed connection of VHP sterilizing installation (3).

3. The in vitro cell culture system of claim 1, wherein the sealing assembly comprises a rectangular groove (22) arranged on the inner wall of the periphery of the opening (16), an inflatable sealing strip (18) is fixedly mounted on the inner wall of the rectangular groove (22), the inner side of the inflatable sealing strip (18) is abutted to the outer side of the incubator (2), a pipeline (19) is fixedly mounted on one side of the inflatable sealing strip (18), an electromagnetic valve (17) is fixedly mounted on the pipeline (19), and the pipeline (19) is connected with an air source of compressed air.

4. The system for in vitro cell culture according to claim 1, wherein a top transparent window (11) is disposed on the top of the first transmission window (1), and the front and the side of the first transmission window (1) are transparent.

5. The system for in vitro cell culture according to claim 1, wherein the bottom of the incubator (2) is fixedly provided with adjustable feet (12) at a rear position.

6. The in vitro cell culture system according to claim 1, wherein a baffle (24) is fixedly installed on the inner wall of the bottom of the first transmission window (1), and one side of the baffle (24) is in contact with one side of the culture box (2).

7. The system for in vitro cell culture according to claim 1, wherein the first transfer window (1) is further provided with a clean air exhaust system (28), a clean air inlet is arranged above the first transfer window (1), and a clean air outlet is arranged below the first transfer window (1).

8. The in vitro cell culture system according to any one of claims 1 to 7, wherein a second transmission window (25) is fixedly and hermetically installed on one side of the first transmission window (1) far away from the first operating glove (6), both sides of the second transmission window (25) are hinged with second transmission window doors (14), and the second transmission window door (14) located on one side of the first transmission window (1) is opened towards the inside of the first transmission window (1); and a second operating glove (26) is fixedly arranged on one side of the first transmission window (1) close to the second transmission window (25).

9. The in vitro cell culture system according to claim 8, wherein a support plate (15) is fixedly installed on one side of the opening of the first transmission window (1), a screw (21) is connected to the top of the support plate (15) in a penetrating manner, a rubber plate (23) is rotatably connected to the bottom of the screw (21), the bottom of the rubber plate (23) is abutted against the top of the incubator (2), and a handle (20) is fixedly installed on the top of the screw (21).

10. The system for in vitro cell culture according to claim 8, wherein the second transfer window (25) is provided with a sterilization device and a clean air exhaust system.

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