CN218115466U

CN218115466U - Cell hypoxic culture operation and operating device

Info

Publication number: CN218115466U
Application number: CN202222122361.6U
Authority: CN
Inventors: 孙建国; 周莉; 李璐
Original assignee: Hangzhou Jiyingjie Biotechnology Co ltd
Current assignee: Hangzhou Jiyingjie Biotechnology Co ltd
Priority date: 2022-08-12
Filing date: 2022-08-12
Publication date: 2022-12-23
Anticipated expiration: 2032-08-12

Abstract

A cell hypoxic culture operation and operating device belongs to the technical field of cell culture. Comprises a shell, an incubator, an operation chamber and a sampling chamber; the incubator and the operation chamber are both arranged at the upper end of the shell, the incubator is positioned at one side of the operation chamber, and a first gas circuit assembly is arranged in the incubator; the sampling chamber is movably arranged in the operating chamber, and a gas circuit assembly II is arranged in the sampling chamber; and a third air path assembly is arranged in the operating chamber, and two operating ports are arranged at the front end of the operating chamber. The utility model discloses a device carries out cell culture and operation under the oxygen deficiency ambient condition, through three gas circuit subassemblies of group, can provide the invariable culture condition of strict oxygen deficiency state and have a work area of systematization, scientization, the operation is cultivateed in this device, can avoid in the past that cell biology contacts oxygen or oxygen deficiency and exists dead danger when the operation.

Description

Cell hypoxic culture operation and operating device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to a cell anoxia cultivates operation and operating means.

Background

Cell culture is an important technical means in detection facilities such as third-party detection, medical facilities such as hospitals and pharmaceutical factories, and research facilities such as universities and research institutes. Wherein the anaerobic cell culture is a bacterial culture and operation under the cell environment condition. The existing cell culture can not maintain the concentration of oxygen in the sampling culture process, so that cell organisms are exposed to oxygen or lack of oxygen during operation to cause death risk, and the culture effect is influenced.

Therefore, a cell hypoxic culture operation and operation device is needed to solve the above technical problems.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell hypoxic is cultivateed operation and operating means to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: a cell hypoxic culture operation and operation device comprises a shell, an incubator, an operation chamber and a sampling chamber; the incubator and the operation chamber are both arranged at the upper end of the shell, the incubator is positioned at one side of the operation chamber, and a first air path assembly is arranged in the incubator; the sampling chamber is movably arranged in the operating chamber, and a gas circuit assembly II is arranged in the sampling chamber; and a third air path assembly is arranged in the operating chamber, and two operating ports are arranged at the front end of the operating chamber.

The sampling chamber also comprises a seed receiving rod sterilizer, a wax melting and disinfecting device, a small door I and rollers; the inoculation rod sterilizer and the wax melting disinfection device are arranged in the sampling chamber; transparent glass is arranged on the small door I; the roller is arranged at the bottom end of the sampling chamber.

The operation room also comprises a box frame, a sterilizing lamp, an operation window and a small door II; the operation window is rotatably arranged on the front side of the operation chamber, two operation ports are arranged at the lower end of the operation window, operation gloves are arranged in the operation ports, and transparent glass is arranged at the upper end of the operation window; the sterilizing lamp is arranged at the top end of the operating room; the box frame is movably arranged in the operating room; the small door II is arranged on one side of the operation chamber and is connected with the incubator.

The incubator also comprises a temperature controller, an incubator and a small door III; the culture box is movably arranged in the incubator; and the small door III is arranged on the front side of the incubator.

The first gas path assembly comprises a first gas port, a first ventilation fan and an oxygen content controller; the first air port and the first ventilation fan are respectively arranged at the upper end and the lower end of the incubator; the oxygen content controller and the air port I are connected with the ventilator I in a control mode.

The second gas circuit component comprises a second gas port, a second ventilation fan and a micro-flow controller; the second air port and the second ventilation fan are respectively arranged at the upper end and the lower end of the sampling chamber; the micro-flow controller and the air port II are in control connection with the ventilator II.

The air path component III comprises an air port III and a ventilation fan III; and the air port III and the ventilation fan III are respectively arranged at the upper end and the lower end of the operation chamber.

The operating table is arranged on the shell, and the bottom end of the shell is provided with a roller; the operation panel sets up in the upper end of incubator.

Compared with the prior art, the beneficial effects of the utility model are that:

the device of the utility model carries out cell culture and operation under the hypoxic environment condition, and three groups of gas circuit components can provide strict culture conditions with constant hypoxic state and a systematic and scientific working area;

the sterilizing lamp is arranged in the device box of the utility model, which can effectively avoid bacterial pollution; meanwhile, the operation window is made of transparent glass, so that the operation condition can be clearly and directly observed, and the plastic gloves are comfortable and flexible to operate and use and convenient to use.

Drawings

FIG. 1 is a schematic diagram showing the internal structure of a cell hypoxic culture operation and handling device;

FIG. 2 is a schematic diagram of a cell hypoxic culture operation and handling device;

FIG. 3 is a schematic side view of a cell hypoxic culture operation and handling device;

FIG. 4 is a schematic view showing the internal structure of the incubator;

in the figure: 1. a housing; 2. an incubator; 3. an operation chamber; 4. a sampling chamber; 5. a first gas path assembly; 6. a second gas path component; 7. a third gas path component; 8. an operation port; 9. a seed-inoculating rod sterilizer; 10. a wax melting and disinfecting device; 11. a first small door; 12. a roller; 13. a box frame; 14. a germicidal lamp; 15. operating a window; 16. a small door II; 17. a culture box; 18. a small door III; 19. a first air port; 20. a first ventilation fan; 21. a second air port; 22, a second ventilator; 23. a gas port III; 24. a third ventilator; 25 operating the platform.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a cell hypoxic culture operation and operation device comprises a shell 1, an incubator 2, an operation chamber 3 and a sampling chamber 4; the incubator 2 and the operation chamber 3 are both arranged at the upper end of the shell 1, the incubator 2 is positioned at one side of the operation chamber 3, and a first air path assembly 5 is arranged in the incubator 2; the sampling chamber 4 is movably arranged in the operating chamber 3, and a second gas circuit assembly 6 is arranged in the sampling chamber 4; and a third air path assembly 7 is arranged in the operation chamber 3, and two operation ports 8 are arranged at the front end of the operation chamber 3.

The sampling chamber 4 also comprises an inoculation rod sterilizer 9, a wax melting disinfection device 10, a small door I11 and rollers 12; the inoculation rod sterilizer 9 and the wax melting and disinfecting device 10 are arranged in the sampling chamber 4; transparent glass is arranged on the small door I11; the roller 12 is arranged at the bottom end of the sampling chamber 4.

The sampling chamber 4 is used for sampling, and the inoculation rod sterilizer 9 and the wax melting disinfection device 10 are arranged to ensure the aseptic operation in the sampling process.

Meanwhile, before use, the second gas circuit component 6 is opened, and the second gas circuit component 6 adjusts the oxygen environment in the sampling chamber 4 to the itching concentration required by the cells.

The operation room 3 also comprises a box frame 13, a germicidal lamp 14, an operation window 15 and a small door II 16; the operation window 15 is rotatably arranged on the front side of the operation chamber 3, two operation ports 8 are arranged at the lower end of the operation window 15, operation gloves are arranged in the operation ports 8, and transparent glass is arranged at the upper end of the operation window 15; the germicidal lamp 14 is arranged at the top end of the operation chamber 3; the box frame 13 is movably arranged in the operation chamber 3; the second small door 16 is arranged on one side of the operating room 3 and is connected with the incubator 2, and the sampled cells can be directly fed into the incubator 2 through the second small door 16, so that the cells are prevented from contacting the external environment and being polluted.

The operating gloves arranged in the operating opening 8 are medical rubber gloves, the gloves can be replaced for use, the integrity of the gloves is guaranteed, and before the gloves are used, the sterilizing lamp 14 in the operating room 3 is turned on to sterilize the gloves.

Meanwhile, before use, the third air path component 7 is opened, and the third air path component 7 adjusts the oxygen environment in the operation chamber 3 to the itching concentration required by the cells.

The operation window 15 is made of transparent glass, the operation condition in the operation chamber 3 can be clearly and directly observed, and the plastic gloves are comfortable and flexible to operate and convenient to use.

The incubator 2 also comprises a temperature controller, an incubator 17 and a small door III 18; the culture box 17 is movably arranged in the incubator 2; the small door three 18 is arranged at the front side of the incubator 2. The temperature controller can accurately and visually control the temperature in the incubator 2, and ensure the activity of cells in culture.

After the culture is completed, the whole culture box 17 is directly taken out through the small door III 18.

The first air path assembly 5 comprises a first air port 19, a first ventilation fan 20 and an oxygen content controller; the first air port 19 and the first ventilation fan 20 are respectively arranged at the upper end and the lower end of the incubator 2, and the first air port 19 and the first ventilation fan 20 are respectively used as an inlet and an outlet of oxygen to continuously exchange the oxygen so as to ensure that the oxygen concentration in the incubator 2 is unchanged; the oxygen content controller is connected with the first air port 19 and the first ventilation fan 20 in a control mode, so that the oxygen concentration is kept constant in the culture process.

The second air path assembly 6 comprises a second air port 21, a second ventilation fan 22 and a micro-flow controller; the second air port 21 and the second ventilation fan 22 are respectively arranged at the upper end and the lower end of the sampling chamber 4, and the second air port 21 and the second ventilation fan 22 are respectively used as an inlet and an outlet of oxygen to continuously exchange the oxygen so as to ensure that the oxygen concentration in the sampling chamber 4 is unchanged; the micro-flow controller and the second air port 21 are in control connection with the second ventilating fan 22, and oxygen concentration in the sampling process is accurately controlled.

The air path assembly III 7 comprises an air port III 23 and a ventilation fan III 24; the third air port 23 and the third ventilation fan 24 are respectively arranged at the upper end and the lower end of the operation chamber 3, and the third air port 23 and the third ventilation fan 24 are respectively used as an inlet and an outlet for oxygen to continuously exchange the oxygen, so that the oxygen concentration in the operation chamber 34 is ensured to be unchanged.

An operating table 25 is arranged on the shell 1, and rollers 12 are arranged at the bottom end of the shell 1; the operation table 25 is provided at the upper end of the incubator 2.

Although the present invention has been shown and described with respect to the implementation of magnetic sensing tiles to the embodiments, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made herein without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell hypoxic culture operation and operation device comprises a shell (1), an incubator (2), an operation chamber (3) and a sampling chamber (4); the device is characterized in that the incubator (2) and the operation chamber (3) are both arranged at the upper end of the shell (1), the incubator (2) is positioned at one side of the operation chamber (3), and a first air circuit assembly (5) is arranged in the incubator (2); the sampling chamber (4) is movably arranged in the operation chamber (3), and a second gas circuit assembly (6) is arranged in the sampling chamber (4); and a third air path assembly (7) is arranged in the operating chamber (3), and two operating ports (8) are arranged at the front end of the operating chamber (3).

2. The device for operating and operating cell-hypoxic culture according to claim 1, characterized in that: the sampling chamber (4) also comprises an inoculation rod sterilizer (9), a wax melting disinfection device (10), a small door I (11) and a roller (12); the inoculation rod sterilizer (9) and the wax melting disinfection device (10) are arranged in the sampling chamber (4); transparent glass is arranged on the small door I (11); the roller (12) is arranged at the bottom end of the sampling chamber (4).

3. The cell culture operation and handling device according to claim 1, wherein: the operation room (3) also comprises a box frame (13), a germicidal lamp (14), an operation window (15) and a small door II (16); the operation window (15) is rotatably arranged on the front side of the operation chamber (3), two operation ports (8) are arranged at the lower end of the operation window (15), operation gloves are arranged in the operation ports (8), and transparent glass is arranged at the upper end of the operation window (15); the sterilizing lamp (14) is arranged at the top end of the operating room (3); the box frame (13) is movably arranged in the operation chamber (3); the second small door (16) is arranged on one side of the operating room (3) and is connected with the incubator (2).

4. The device for operating and operating cell-hypoxic culture according to claim 1, characterized in that: the incubator (2) also comprises a temperature controller, an incubator box (17) and a small door III (18); the culture box (17) is movably arranged in the culture box (2); the small door III (18) is arranged on the front side of the incubator (2).

5. The device for operating and operating cell-hypoxic culture according to claim 1, characterized in that: the first air path assembly (5) comprises a first air port (19), a first ventilation fan (20) and an oxygen content controller; the air port I (19) and the ventilation fan I (20) are respectively arranged at the upper end and the lower end of the incubator (2); the oxygen content controller and the air port I (19) are in control connection with the ventilator I (20).

6. The device for operating and operating cell-hypoxic culture according to claim 1, characterized in that: the second air path assembly (6) comprises a second air port (21), a second ventilator (22) and a micro-flow controller; the second air port (21) and the second ventilation fan (22) are respectively arranged at the upper end and the lower end of the sampling chamber (4); and the micro-flow controller and the air outlet II (21) are in control connection with the ventilator II (22).

7. The device for operating and operating cell-hypoxic culture according to claim 1, characterized in that: the third air path assembly (7) comprises a third air port (23) and a third ventilation fan (24); and the air port III (23) and the ventilation fan III (24) are respectively arranged at the upper end and the lower end of the operation chamber (3).

8. The cell culture operation and handling device according to claim 1, wherein: an operating table (25) is arranged on the shell (1), and rollers (12) are arranged at the bottom end of the shell (1); the operating platform (25) is arranged at the upper end of the incubator (2).