CN212159256U - Experimental apparatus is gathered to beehive bioaerosol - Google Patents
Experimental apparatus is gathered to beehive bioaerosol Download PDFInfo
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- CN212159256U CN212159256U CN202020913870.9U CN202020913870U CN212159256U CN 212159256 U CN212159256 U CN 212159256U CN 202020913870 U CN202020913870 U CN 202020913870U CN 212159256 U CN212159256 U CN 212159256U
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Abstract
The utility model relates to a beehive bioaerosol collection experiment device, which comprises a first aerosol collection box, a second aerosol collection box and a third aerosol collection box, wherein the first aerosol collection box is provided with a first air inlet and a first air outlet; and air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere. The aerosol collection experimental device realizes collection of the aerosol of the beehive, provides favorable technical support for verification of the conclusion, indicates directions for prevention and control of the bee colony infectious diseases, and is favorable for research on the infection law of the gas source sexually transmitted diseases of the bee colony and prevention and treatment measures.
Description
Technical Field
The utility model relates to a bee-keeping technical field especially relates to a beehive bioaerosol gathers experimental apparatus.
Background
The beehive is a place for bees to live and propagate and develop various production activities in the beekeeping production. The density of the polypide in the beehive is high, the temperature is high, the humidity is high, and the stored substances such as low-concentration nectar, pollen, polypide and the like are beneficial to the growth and the propagation of pathogens.
At present, bee colony diseases are serious, including infectious diseases such as bacterial diseases, virus diseases and mycosis, and great challenges are brought to the bee-keeping industry. Aerosols are an important mode of transmission of pathogenic microorganisms, which are suspended in air and spread by airflow to contact the host causing disease. According to the conjecture, the spreading of various infectious diseases in bee colonies is also the transmission of aerosol carrying germs through airflow, and in order to verify the view, a beehive biological aerosol collection experimental device is provided.
SUMMERY OF THE UTILITY MODEL
The utility model provides a beehive bioaerosol collection experiment device aiming at the defects of the prior art.
The utility model is realized by the following technical scheme, the utility model provides a beehive bioaerosol collection experiment device, which comprises a first aerosol collection box, a second aerosol collection box and a third aerosol collection box, wherein the first aerosol collection box is provided with a first air inlet and a first air outlet, the second aerosol collection box is provided with a second air inlet and a second air outlet, and the third aerosol collection box is provided with a third air inlet and a third air outlet; air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere; the first air outlet is communicated with the second air inlet through a first connecting pipeline, and a first switch valve is connected to the first connecting pipeline; a second connecting pipeline is communicated with the first connecting pipeline, the tail end of the second connecting pipeline is connected with a third air inlet, and a second switch valve is connected to the second connecting pipeline; the first switch valve is positioned between the second connecting pipeline and the second aerosol collecting box; the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all connected with a gas sampling device; beehives filled with sick bee colonies are placed in the first aerosol collecting box, and beehives filled with healthy bee colonies are placed in the second aerosol collecting box and the third aerosol collecting box.
Preferably, the gas sampling device comprises a gas sampling bottle and a vacuum pump, wherein a gas inlet pipe of the gas sampling bottle is communicated with the aerosol collection box, and a gas outlet pipe of the gas sampling bottle is connected with a gas inlet end of the vacuum pump; phosphate buffer saline solution is filled in the gas sampling bottle.
Preferably, the first air inlet is connected with a positive pressure fan.
Preferably, a beehive containing a healthy colony of bees is also placed within the first aerosol collection chamber.
Preferably, the air filtering mechanism is a glass fiber filter membrane.
Preferably, the first aerosol collection box, the second aerosol collection box and the third aerosol collection box are all provided with box doors.
The utility model has the advantages that:
1. the aerosol collection experimental device realizes collection of the aerosol of the beehive, provides favorable technical support for verification of the conclusion, indicates directions for prevention and control of the bee colony infectious diseases, and is favorable for research on the infection law of the gas source sexually transmitted diseases of the bee colony and prevention and treatment measures.
2. The aerosol in the aerosol collection box is sucked into the gas sampling bottle through the vacuum pump, the microbial aerosol in the airflow is intercepted through the phosphate buffer solution, subsequently, the aerosol particles are precipitated through ultracentrifugation, and the types of the microbes in the aerosol are measured through PCR.
Drawings
FIG. 1 is a schematic view of the structure of the present invention
Shown in the figure:
1. first aerosol collection box, 2, first air inlet, 3, air filter mechanism, 4, beehive, 5, first connecting pipeline, 6, first switch valve, 7, second switch valve, 8, second connecting pipeline, 9, first gas outlet, 10, second air inlet, 11, second aerosol collection box, 12, second gas outlet, 13, third gas outlet, 14, third gas inlet, 15, third aerosol collection box, 16, gas sampling bottle, 17, air inlet pipe, 18, air outlet pipe.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.
As shown in fig. 1, the utility model discloses a first aerosol collection box 1, second aerosol collection box 11 and third aerosol collection box 15 are equipped with first air inlet 2 and first gas outlet 9 on the first aerosol collection box 1, are equipped with second air inlet 10 and second gas outlet 12 on the second aerosol collection box 11, are equipped with third air inlet 14 and third gas outlet 13 on the third aerosol collection box 15. The air filtering mechanisms 3 are respectively arranged at the first air inlet 2, the third air inlet 14, the third air outlet 13 and the second air outlet 12, and the first air inlet 2, the third air outlet 13 and the second air outlet 12 are communicated with the outside atmosphere. The first air outlet 9 is communicated with the second air inlet 10 through a first connecting pipeline 5, and the first connecting pipeline 5 is connected with a first switch valve 6. The first connecting pipeline 5 is communicated with a second connecting pipeline 8, the tail end of the second connecting pipeline 8 is connected with a third air inlet 14, and the second connecting pipeline 8 is connected with a second switch valve 7. The first on-off valve 6 is located between the second connecting pipe 8 and the second aerosol collection box 11.
And the first aerosol collecting box 1, the second aerosol collecting box 11 and the third aerosol collecting box 15 are all connected with a gas sampling device. Beehives 4 containing sick bee colonies are placed in the first aerosol collection box 1, and beehives 4 containing healthy bee colonies are placed in the second aerosol collection box 11 and the third aerosol collection box 15.
In the present embodiment, the length of each of the first connecting duct 5 and the second connecting duct 8 is about 1 meter.
In this embodiment, the gas sampling device includes a gas sampling bottle 16 and a vacuum pump (not shown), an air inlet pipe 17 of the gas sampling bottle 16 is communicated with the aerosol collection box, and an air outlet pipe 18 of the gas sampling bottle 16 is connected with an air inlet end of the vacuum pump. A phosphate buffered saline solution is filled in the gas sampling bottle 16. The aerosol in the aerosol collection box is sucked into the gas sampling bottle 16 through a vacuum pump, the microbial aerosol in the airflow is intercepted through phosphate buffer solution, subsequently, the aerosol particles are precipitated through ultracentrifugation, and the types of the microbes in the aerosol are measured through PCR. The gas sampling bottle 16 and the vacuum pump used in the embodiment are both of the existing structure, and the start and stop of the vacuum pump are controlled manually. In this embodiment, the gas flow rate of the vacuum pump is set to 1L/min to 5L/min.
In this embodiment, in order to accelerate the air flow in the first aerosol collection box 1 to flow into the second aerosol collection box 11 and the third aerosol collection box 15, the first air inlet 2 is connected to a positive pressure fan (not shown), and the positive pressure fan can be manually turned on according to the use requirement.
In this example, to further verify the accuracy of the test conclusions, beehives containing healthy bee colonies were also placed inside the first aerosol collection chamber 1. After a period of time, it can be further verified by testing the health of healthy bee colonies that the aerosol is carried to the point where pathogens can be transmitted through the airflow.
In this embodiment, the air filtering mechanism 3 is a glass fiber filter membrane, and the glass fiber filter membrane can trap pathogenic microorganisms in the air.
In this embodiment, in order to facilitate the beehive to be placed into the aerosol collection box, box doors are respectively arranged on the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15, and the beehive is placed into the aerosol collection box from the box doors.
When the device is used specifically, the corresponding beehives are respectively placed into the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15, and the first switch valve 6 and the second switch valve 7 are opened to enable air in the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15 to flow freely. After a period of time, the first switch valve 6 and the second switch valve 7 are closed, and the aerosol in the corresponding aerosol collection box is collected through the gas sampling device connected with each aerosol collection box.
The aerosol in each gas sampling bottle 16 is analyzed and matched with the health condition check of bees in each beehive, and the final result is that: pathogenic microorganisms are detected in the gas sampling bottle 16 corresponding to the first aerosol collection box 1, and meanwhile, diseased bees appear in the beehive filled with healthy bee colonies in the first aerosol collection box 1. Pathogenic microorganisms are detected in the gas sampling bottle 16 corresponding to the second aerosol collection box 11, and meanwhile, diseased bees appear in the beehive filled with healthy bee colonies in the second aerosol collection box 11. Pathogenic microorganisms are intercepted by the glass fiber filter membrane of the gas sampling bottle 16 corresponding to the third aerosol collection box 15, so that the pathogenic microorganisms are not detected, and meanwhile, no diseased bees appear in the beehive filled with healthy bee colonies in the third aerosol collection box 15. Thereby verifying the assumption that pathogens carried by the aerosol can propagate through the airflow.
Of course, the above description is not limited to the above examples, and technical features of the present invention that are not described in the present application may be implemented by or using the prior art, and are not described herein again; the above embodiments and drawings are only used for illustrating the technical solutions of the present invention and are not intended to limit the present invention, and the present invention has been described in detail with reference to the preferred embodiments, and those skilled in the art should understand that changes, modifications, additions or substitutions made by those skilled in the art within the spirit of the present invention should also belong to the protection scope of the claims of the present invention.
Claims (6)
1. The utility model provides an experimental apparatus is gathered to beehive bioaerosol which characterized in that: the aerosol collection box is provided with a first air inlet and a first air outlet, the second aerosol collection box is provided with a second air inlet and a second air outlet, and the third aerosol collection box is provided with a third air inlet and a third air outlet; air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere; the first air outlet is communicated with the second air inlet through a first connecting pipeline, and a first switch valve is connected to the first connecting pipeline; a second connecting pipeline is communicated with the first connecting pipeline, the tail end of the second connecting pipeline is connected with a third air inlet, and a second switch valve is connected to the second connecting pipeline; the first switch valve is positioned between the second connecting pipeline and the second aerosol collecting box; the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all connected with a gas sampling device; beehives filled with sick bee colonies are placed in the first aerosol collecting box, and beehives filled with healthy bee colonies are placed in the second aerosol collecting box and the third aerosol collecting box.
2. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: the gas sampling device comprises a gas sampling bottle and a vacuum pump, wherein a gas inlet pipe of the gas sampling bottle is communicated with the aerosol collection box, and a gas outlet pipe of the gas sampling bottle is connected with a gas inlet end of the vacuum pump; phosphate buffer saline solution is filled in the gas sampling bottle.
3. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: the first air inlet is connected with the positive pressure fan.
4. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: a beehive filled with healthy bee colonies is also placed in the first aerosol collection box.
5. The experimental apparatus for collecting bio-aerosol of beehive according to claim 2, wherein: the air filtering mechanism is a glass fiber filter membrane.
6. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: and the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all provided with box doors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020913870.9U CN212159256U (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202020913870.9U CN212159256U (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
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| Publication Number | Publication Date |
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| CN212159256U true CN212159256U (en) | 2020-12-15 |
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| CN202020913870.9U Active CN212159256U (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
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| CN (1) | CN212159256U (en) |
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2020
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