CN214309773U - Hierarchical sampling device of cavy expiratory aerosol - Google Patents
Hierarchical sampling device of cavy expiratory aerosol Download PDFInfo
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- CN214309773U CN214309773U CN202120311016.XU CN202120311016U CN214309773U CN 214309773 U CN214309773 U CN 214309773U CN 202120311016 U CN202120311016 U CN 202120311016U CN 214309773 U CN214309773 U CN 214309773U
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Images
Abstract
A guinea pig exhaled aerosol graded sampling device comprises a guinea pig fixing system, a guinea pig exhaled aerosol monitoring system and a guinea pig exhaled microorganism aerosol graded sampling system; the guinea pig fixing system comprises a cabin body, two high-efficiency filtering devices and four guinea pig fixing devices; the guinea pig exhaled aerosol monitoring system is communicated with the guinea pig fixing system and can be switched for use; the method is used for monitoring the aerosol particle concentration and particle size distribution characteristics in the exhaled air in real time. The utility model discloses a device has filled the technique blank of unable accurate among the prior art, real-time analysis animal exhalation aerosol particle diameter spectrum, can effectively reduce the influence of environment aerosol particle to the experimental result, not only can analyze total particulate matter concentration and particle size distribution characteristic in the cavy exhalation, can also carry out microorganism particle size grading sampling, and the concentration and the particle size distribution characteristic of analysis microorganism aerosol are favorable to deepening the epidemic and the propagation mechanism of systematic study microorganism.
Description
Technical Field
The utility model belongs to the technical field of pathogenic microorganism aerosol sampling, a cavy exhaled aerosol grading collection system is related to.
Background
Aerosols (Aerosol) are stable dispersions of particles and air in which solid or liquid particles are suspended for extended periods of time in the air. If the particles contain microorganisms, a microbial aerosol is formed. They can be classified into viral aerosols (viral aerosols), bacterial aerosols (bacterial aerosols) and fungal aerosols (fungal aerosols) according to the type of microorganism. The particle size range that a person can call in is 0.5-5 μm. Since these particles are themselves small enough, the settling velocity in a gaseous medium is low, they can be suspended in air for long periods of time, and they can drift away with the wind. The wide spread and increased concentration of the microbial aerosol can cause atmospheric environmental pollution, so that the probability of outbreak of certain infectious diseases is increased, and the microbial aerosol poses serious threats to human health, normal life and normal production of animal husbandry.
Influenza virus is an important respiratory tract transmission pathogen, has wide host range, high mutation speed and strong transmission capability, even causes pandemics in the world for many times, and brings great harm to human health and livestock and poultry breeding industry. The guinea pig is one of important animal models for evaluating the transmission capability of influenza viruses among mammals, and the understanding of the aerosol detoxification rule of the guinea pig infected with the influenza viruses has important reference significance for scientifically judging the transmission capability and comprehensive prevention and control of the influenza viruses. However, no experimental device capable of monitoring aerosol particles exhaled by guinea pigs in real time and collecting the aerosol particles in a particle size grading manner exists at present.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that to prior art not enough, provide one kind and can monitor the guinea pig of testee exhalation aerosol concentration and particle size distribution characteristic to carry out the animal experimental apparatus of microorganism aerosol particle size grading sampling.
In order to solve the technical problem, the utility model discloses the technical scheme who takes as follows:
a guinea pig exhaled aerosol graded sampling device comprises a guinea pig fixing system, a guinea pig exhaled aerosol monitoring system and a guinea pig exhaled microorganism aerosol graded sampling system;
the guinea pig fixing system is used as a fixing and placing device of a tested guinea pig and comprises a cabin body, a high-efficiency filtering device and a guinea pig fixing device; the front surface of the bin body is provided with an isolation observation window and an operation window, and the center of the bottom surface is provided with a sampling port; the high-efficiency filtering devices are positioned at two sides of the top of the bin body to filter air microorganisms and finally convey sterile air into the bin body; the guinea pig fixing devices are 4 in number and are arranged at the central position of the cabin body, and after the guinea pig is fixed, the heads of the guinea pig fixing devices face to a sampling port in the center of the bottom surface.
The guinea pig exhaled aerosol monitoring system is communicated with the guinea pig fixing system and is used for monitoring the concentration and particle size distribution characteristics of aerosol particles in exhaled air in real time. The exhaled aerosol monitoring system comprises a particle size spectrometer and a controller; the particle size spectrometer can monitor the aerodynamic particle size of aerosol particles in the animal fixing system in real time or at different time intervals, and gives the distribution conditions of the quantity concentration, the surface area concentration, the volume concentration and the mass concentration of the aerosol along with the particle size; the controller is connected with the particle size spectrometer, controls the sampling frequency and the sampling time of the particle size spectrometer, and reads analysis data.
The guinea pig exhaled microbe aerosol grading sampling system is communicated with the guinea pig fixing system, shares the same communication interface with the guinea pig exhaled aerosol monitoring system, can be switched to use, is used for collecting guinea pig exhaled gas in a grain size grading manner, and analyzes the concentration and grain size distribution characteristics of microbes in the exhaled gas. The graded sampling system comprises an Anderson six-grade sampler and a sampling pump; the Anderson six-level sampler divides aerosol particles into 6 particle size sections according to particle size distribution, and the particle size sections are respectively as follows:
greater than 7 μm; 4.7-7 μm; 3.3-4.7 μm; 2.1-3.3 μm; 1.1-2.1 μm; 0.65-1.1 μm
Sampling each particle size section respectively to obtain an aerosol sample; the sampling pump is connected with the Anderson six-level sampler, provides a sampling power source for the Anderson six-level sampler and controls sampling time.
The novel garbage bin is further characterized in that a light source is arranged at the top of the bin body and comprises an LED lamp and an ultraviolet lamp; the LED lamp is used for illumination, and the ultraviolet lamp is used for sterilization;
the device is further characterized in that the particle size spectrometer and the Anderson six-level sampler are respectively communicated with an animal fixing system through rubber hoses.
The utility model discloses a device has filled the technique blank of unable accurate among the prior art, real-time analysis animal exhalation aerosol particle diameter spectrum, can effectively reduce the influence of environment aerosol particle to the experimental result, not only can analyze total particulate matter concentration and particle size distribution characteristic in the cavy exhalation, can also carry out microorganism particle size grading sampling, and the concentration and the particle size distribution characteristic of analysis microorganism aerosol are favorable to deepening the epidemic and the propagation mechanism of systematic study microorganism.
Drawings
Fig. 1 is a schematic structural diagram of the guinea pig exhaled aerosol fractional sampling device.
Fig. 2 is a top plan view of the guinea pig exhaled breath aerosol analysis sampling apparatus in the cartridge body.
In the figure: 1, a cabin body; 2, a high-efficiency filtering device A; 3, a high-efficiency filtering device B; 4 guinea pig fixation device a;
5 guinea pig fixation device B; 6 guinea pig fixation device C; 7 guinea pig fixation device D; 8 a front panel of the bin body; 9 operating the window A; 10 operating the window B; 11 a sampling connecting port; 12 light source; 13 guinea pig exhalation aerosol monitoring system; 14 Anderson six-stage sampler; 15 sampling pump.
Detailed Description
The present invention will be further described in detail with reference to the drawings and the following detailed description.
As shown in fig. 1, the guinea pig exhaled aerosol graded sampling experimental device comprises an animal fixing system and a guinea pig exhaled aerosol monitoring system or a guinea pig exhaled microorganism aerosol graded sampling system which are connected through a 25mm diameter rubber hose; the guinea pig exhaled aerosol monitoring system and the guinea pig exhaled microorganism aerosol graded sampling system share the same connecting interface and can be used alternately; the guinea pig fixing system is of a cabin structure, and the inside of the fixing system is used as a fixed placing space and an operation space for experimenters of the tested guinea pigs; the guinea pig exhaled aerosol monitoring system is communicated with the guinea pig fixing system and is used for monitoring the concentration and particle size distribution characteristics of aerosol particles in exhaled air in real time; the guinea pig exhaled microorganism aerosol grading sampling system is communicated with the guinea pig fixing system and is used for collecting guinea pig exhaled gas in a grading mode according to particle sizes and analyzing the concentration and particle size distribution characteristics of microorganisms in the exhaled gas.
Specifically, the animal fixing system comprises a cabin body 1 (with the length of 1m, the width of 0.8m and the height of 0.6m), a high-efficiency filtering device and a guinea pig fixing device; the front panel 8 of the bin body is made of transparent material and is used as an observation window. Meanwhile, the front panel can be opened to be used as an entrance and exit of the experimental animal; the front panel is provided with an operation window A and an operation window B, and both the operation windows are provided with rubber gloves; the two high-efficiency filtering devices are positioned at two sides of the top of the bin body and used for filtering air microorganisms and finally conveying sterile air into the bin body.
A sampling connecting port 11 is arranged at the right center of the bottom of the bin body 1; four guinea pig fixing devices are arranged at the bottom in the cabin body and used for fixing four guinea pigs in the cabin body, and the mouths and noses of the four guinea pigs face to the sampling connecting port 11 positioned in the center after the four guinea pigs are fixed; the sampling connecting port is used for communicating a guinea pig exhaled aerosol monitoring system or a guinea pig exhaled microorganism aerosol grading sampling system.
A light source 12 is arranged at the top in the bin body 1, the light source 12 comprises an LED lamp and an ultraviolet lamp, the LED lamp is used for illumination, and the ultraviolet lamp is used for sterilization; the light source 12 is connected to an external power source.
Guinea pig exhaled aerosol monitoring system 13(TSI3321) was purchased from TSI corporation, usa, and was used to monitor the aerodynamic particle size of aerosol particles in an animal immobilization system in real time and give the distribution of aerosol number concentration, surface area concentration, volume concentration and mass concentration with particle size.
The guinea pig exhaled microbe aerosol graded sampling system comprises an Anderson six-level sampler 14 and a sampling pump 15; the Anderson six-stage sampler 14 is purchased from Tisch company in America and is used for dividing aerosol particles exhaled by guinea pigs into 6 different particle size intervals for collection so as to analyze the characteristics of the aerosol particles exhaled by the guinea pigs; the sampling pump 15(ZR-2000) is purchased from Guangzhong intelligent instrument Limited in Qingdao, is connected with the Anderson six-level sampler 14 through a plastic hose, provides a sampling power source (28.3L/min) for the Anderson six-level sampler 14 and controls sampling time.
During work, the front panel 8 of the cabin body 1 is opened, the anesthetized guinea pig to be tested is placed in the cabin body, the front panel is closed, and the guinea pig is fixed in the guinea pig fixing device through the rubber gloves of the operation window. After the smell of the guinea pigs is stable, firstly connecting a guinea pig exhaled aerosol monitoring system 13 to a sampling interface 11 at the bottom of a cabin body, and continuously monitoring for 5 minutes to obtain aerosol particle size spectrum characteristics in the exhaled gas of the guinea pigs; then, the guinea pig exhaled aerosol monitoring system 13 is dismounted, the Anderson six-level sampler 14 is connected to the sampling interface 11 at the bottom of the cabin body, continuous sampling is carried out for 30 minutes, and the concentration and the particle size distribution of the exhaled microorganism aerosol of the guinea pig are analyzed by measuring the content of microorganisms in the sampled samples; when the continuous monitoring or continuous sampling is carried out, negative pressure is generated in the experimental bin body, air microorganisms are filtered through the high-efficiency filter, and finally sterile air is conveyed into the bin body, and directional airflow from the top to the bottom and from the periphery to the center is formed. During the experiment, the behavior and mental state of the tested animal in the bin are observed through the transparent panel 8 on the front surface of the bin body 1, and the test operation in the bin is carried out through the operation window; the LED and ultraviolet lamps 12 provide the required illumination and disinfection in the chamber for the experimental animals.
Claims (3)
1. A guinea pig exhaled aerosol graded sampling device is characterized by comprising a guinea pig fixing system, a guinea pig exhaled aerosol monitoring system and a guinea pig exhaled microorganism aerosol graded sampling system;
the guinea pig fixing system is used as a fixing and placing device of a tested guinea pig and comprises a cabin body, a high-efficiency filtering device and a guinea pig fixing device; the front surface of the bin body is provided with an isolation observation window and an operation window, and the center of the bottom surface is provided with a sampling port; the high-efficiency filtering devices are positioned on two sides of the top of the bin body; the number of the guinea pig fixing devices is 4, and the guinea pig fixing devices are arranged at the central position of the cabin body;
the guinea pig exhaled aerosol monitoring system is in communication with a guinea pig fixation system; the exhaled aerosol monitoring system comprises a particle size spectrometer and a controller; the particle size spectrometer monitors the aerodynamic particle size of aerosol particles in the animal fixation system in real time or at different time intervals; the controller is connected with the particle size spectrometer, controls the sampling frequency and the sampling time of the particle size spectrometer, and reads analysis data;
the guinea pig exhaled microbe aerosol graded sampling system is communicated with the guinea pig fixing system, shares the same communication interface with the guinea pig exhaled aerosol monitoring system, and is switched for use; the graded sampling system comprises an Anderson six-grade sampler and a sampling pump; the sampling pump is connected with the Anderson six-level sampler, provides a sampling power source for the Anderson six-level sampler and controls sampling time.
2. The hierarchical guinea pig exhaled aerosol sampling device according to claim 1, wherein a light source is provided at the top of the chamber body, the light source comprising an LED lamp and an ultraviolet lamp; the LED lamp is used for illumination, and the ultraviolet lamp is used for sterilization.
3. The apparatus for fractional sampling of exhaled breath sol from guinea pigs according to claim 1 or 2, wherein the particle size spectrometer and the Anderson six-stage sampler are each in communication with an animal holding system via a rubber hose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120311016.XU CN214309773U (en) | 2021-02-03 | 2021-02-03 | Hierarchical sampling device of cavy expiratory aerosol |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120311016.XU CN214309773U (en) | 2021-02-03 | 2021-02-03 | Hierarchical sampling device of cavy expiratory aerosol |
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| Publication Number | Publication Date |
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| CN214309773U true CN214309773U (en) | 2021-09-28 |
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| CN202120311016.XU Expired - Fee Related CN214309773U (en) | 2021-02-03 | 2021-02-03 | Hierarchical sampling device of cavy expiratory aerosol |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114343723A (en) * | 2021-12-22 | 2022-04-15 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
-
2021
- 2021-02-03 CN CN202120311016.XU patent/CN214309773U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114343723A (en) * | 2021-12-22 | 2022-04-15 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
| CN114343723B (en) * | 2021-12-22 | 2022-11-22 | 北京华泰诺安技术有限公司 | System and method for non-invasive simultaneous collection of human exhaled aerosol and gas |
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