CN111487095A - Experimental apparatus is gathered to beehive bioaerosol - Google Patents
Experimental apparatus is gathered to beehive bioaerosol Download PDFInfo
- Publication number
- CN111487095A CN111487095A CN202010458641.7A CN202010458641A CN111487095A CN 111487095 A CN111487095 A CN 111487095A CN 202010458641 A CN202010458641 A CN 202010458641A CN 111487095 A CN111487095 A CN 111487095A
- Authority
- CN
- China
- Prior art keywords
- aerosol
- air outlet
- collecting box
- air inlet
- beehive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2226—Sampling from a closed space, e.g. food package, head space
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2202—Devices for withdrawing samples in the gaseous state involving separation of sample components during sampling
- G01N2001/222—Other features
- G01N2001/2223—Other features aerosol sampling devices
Abstract
The invention relates to a beehive biological aerosol collecting experimental device which comprises a first aerosol collecting box, a second aerosol collecting box and a third aerosol collecting box, wherein the first aerosol collecting box is provided with a first air inlet and a first air outlet; and air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere. The aerosol collection experimental device realizes collection of the aerosol of the beehive, provides favorable technical support for verification of the conclusion, indicates directions for prevention and control of the bee colony infectious diseases, and is favorable for research on the infection law of the gas source sexually transmitted diseases of the bee colony and prevention and treatment measures.
Description
Technical Field
The invention relates to the technical field of beekeeping, in particular to a beehive bioaerosol collection experimental device.
Background
The beehive is a place for bees to live and propagate and develop various production activities in the beekeeping production. The density of the polypide in the beehive is high, the temperature is high, the humidity is high, and the stored substances such as low-concentration nectar, pollen, polypide and the like are beneficial to the growth and the propagation of pathogens.
At present, bee colony diseases are serious, including infectious diseases such as bacterial diseases, virus diseases and mycosis, and great challenges are brought to the bee-keeping industry. Aerosols are an important mode of transmission of pathogenic microorganisms, which are suspended in air and spread by airflow to contact the host causing disease. According to the conjecture, the spreading of various infectious diseases in bee colonies is also the transmission of aerosol carrying germs through airflow, and in order to verify the view, a beehive biological aerosol collection experimental device is provided.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a beehive bioaerosol collection experimental device.
The invention is realized by the following technical scheme, and provides a beehive biological aerosol collecting experimental device which comprises a first aerosol collecting box, a second aerosol collecting box and a third aerosol collecting box, wherein the first aerosol collecting box is provided with a first air inlet and a first air outlet; air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere; the first air outlet is communicated with the second air inlet through a first connecting pipeline, and a first switch valve is connected to the first connecting pipeline; a second connecting pipeline is communicated with the first connecting pipeline, the tail end of the second connecting pipeline is connected with a third air inlet, and a second switch valve is connected to the second connecting pipeline; the first switch valve is positioned between the second connecting pipeline and the second aerosol collecting box; the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all connected with a gas sampling device; beehives filled with sick bee colonies are placed in the first aerosol collecting box, and beehives filled with healthy bee colonies are placed in the second aerosol collecting box and the third aerosol collecting box.
Preferably, the gas sampling device comprises a gas sampling bottle and a vacuum pump, wherein a gas inlet pipe of the gas sampling bottle is communicated with the aerosol collection box, and a gas outlet pipe of the gas sampling bottle is connected with a gas inlet end of the vacuum pump; phosphate buffer saline solution is filled in the gas sampling bottle.
Preferably, the first air inlet is connected with a positive pressure fan.
Preferably, a beehive containing a healthy colony of bees is also placed within the first aerosol collection chamber.
Preferably, the air filtering mechanism is a glass fiber filter membrane.
Preferably, the first aerosol collection box, the second aerosol collection box and the third aerosol collection box are all provided with box doors.
The invention has the beneficial effects that:
1. the aerosol collection experimental device realizes collection of the aerosol of the beehive, provides favorable technical support for verification of the conclusion, indicates directions for prevention and control of the bee colony infectious diseases, and is favorable for research on the infection law of the gas source sexually transmitted diseases of the bee colony and prevention and treatment measures.
2. The aerosol in the aerosol collection box is sucked into the gas sampling bottle through the vacuum pump, the microbial aerosol in the airflow is intercepted through the phosphate buffer solution, subsequently, the aerosol particles are precipitated through ultracentrifugation, and the types of the microbes in the aerosol are measured through PCR.
Drawings
FIG. 1 is a schematic view of the structure of the present invention
Shown in the figure:
1. first aerosol collection box, 2, first air inlet, 3, air filter mechanism, 4, beehive, 5, first connecting pipeline, 6, first switch valve, 7, second switch valve, 8, second connecting pipeline, 9, first gas outlet, 10, second air inlet, 11, second aerosol collection box, 12, second gas outlet, 13, third gas outlet, 14, third gas inlet, 15, third aerosol collection box, 16, gas sampling bottle, 17, air inlet pipe, 18, air outlet pipe.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.
As shown in fig. 1, the aerosol collection box comprises a first aerosol collection box 1, a second aerosol collection box 11 and a third aerosol collection box 15, wherein the first aerosol collection box 1 is provided with a first air inlet 2 and a first air outlet 9, the second aerosol collection box 11 is provided with a second air inlet 10 and a second air outlet 12, and the third aerosol collection box 15 is provided with a third air inlet 14 and a third air outlet 13. The air filtering mechanisms 3 are respectively arranged at the first air inlet 2, the third air inlet 14, the third air outlet 13 and the second air outlet 12, and the first air inlet 2, the third air outlet 13 and the second air outlet 12 are communicated with the outside atmosphere. The first air outlet 9 is communicated with the second air inlet 10 through a first connecting pipeline 5, and the first connecting pipeline 5 is connected with a first switch valve 6. The first connecting pipeline 5 is communicated with a second connecting pipeline 8, the tail end of the second connecting pipeline 8 is connected with a third air inlet 14, and the second connecting pipeline 8 is connected with a second switch valve 7. The first on-off valve 6 is located between the second connecting pipe 8 and the second aerosol collection box 11.
And the first aerosol collecting box 1, the second aerosol collecting box 11 and the third aerosol collecting box 15 are all connected with a gas sampling device. Beehives 4 containing sick bee colonies are placed in the first aerosol collection box 1, and beehives 4 containing healthy bee colonies are placed in the second aerosol collection box 11 and the third aerosol collection box 15.
In the present embodiment, the length of each of the first connecting duct 5 and the second connecting duct 8 is about 1 meter.
In the embodiment, the gas sampling device comprises a gas sampling bottle 16 and a vacuum pump (not marked in the figure), wherein a gas inlet pipe 17 of the gas sampling bottle 16 is communicated with an aerosol collection box, a gas outlet pipe 18 of the gas sampling bottle 16 is connected with a gas inlet end of the vacuum pump, phosphate buffer saline solution is filled in the gas sampling bottle 16, the aerosol in the aerosol collection box is sucked into the gas sampling bottle 16 through the vacuum pump, microbial aerosol in the gas flow is intercepted through the phosphate buffer saline solution, aerosol particles are subsequently precipitated through ultracentrifugation, and microbial species in the aerosol are determined through PCR (polymerase chain reaction).
In this embodiment, in order to accelerate the air flow in the first aerosol collection box 1 to flow into the second aerosol collection box 11 and the third aerosol collection box 15, the first air inlet 2 is connected to a positive pressure fan (not shown), and the positive pressure fan can be manually turned on according to the use requirement.
In this example, to further verify the accuracy of the test conclusions, beehives containing healthy bee colonies were also placed inside the first aerosol collection chamber 1. After a period of time, it can be further verified by testing the health of healthy bee colonies that the aerosol is carried to the point where pathogens can be transmitted through the airflow.
In this embodiment, the air filtering mechanism 3 is a glass fiber filter membrane, and the glass fiber filter membrane can trap pathogenic microorganisms in the air.
In this embodiment, in order to facilitate the beehive to be placed into the aerosol collection box, box doors are respectively arranged on the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15, and the beehive is placed into the aerosol collection box from the box doors.
When the device is used specifically, the corresponding beehives are respectively placed into the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15, and the first switch valve 6 and the second switch valve 7 are opened to enable air in the first aerosol collection box 1, the second aerosol collection box 11 and the third aerosol collection box 15 to flow freely. After a period of time, the first switch valve 6 and the second switch valve 7 are closed, and the aerosol in the corresponding aerosol collection box is collected through the gas sampling device connected with each aerosol collection box.
The aerosol in each gas sampling bottle 16 is analyzed and matched with the health condition check of bees in each beehive, and the final result is that: pathogenic microorganisms are detected in the gas sampling bottle 16 corresponding to the first aerosol collection box 1, and meanwhile, diseased bees appear in the beehive filled with healthy bee colonies in the first aerosol collection box 1. Pathogenic microorganisms are detected in the gas sampling bottle 16 corresponding to the second aerosol collection box 11, and meanwhile, diseased bees appear in the beehive filled with healthy bee colonies in the second aerosol collection box 11. Pathogenic microorganisms are intercepted by the glass fiber filter membrane of the gas sampling bottle 16 corresponding to the third aerosol collection box 15, so that the pathogenic microorganisms are not detected, and meanwhile, no diseased bees appear in the beehive filled with healthy bee colonies in the third aerosol collection box 15. Thereby verifying the assumption that pathogens carried by the aerosol can propagate through the airflow.
Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments and drawings are only for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that changes, modifications, additions or substitutions within the spirit and scope of the present invention may be made by those skilled in the art without departing from the spirit of the present invention, and shall also fall within the scope of the claims of the present invention.
Claims (6)
1. The utility model provides an experimental apparatus is gathered to beehive bioaerosol which characterized in that: the aerosol collection box is provided with a first air inlet and a first air outlet, the second aerosol collection box is provided with a second air inlet and a second air outlet, and the third aerosol collection box is provided with a third air inlet and a third air outlet; air filtering mechanisms are respectively arranged at the first air inlet, the third air outlet and the second air outlet, and the first air inlet, the third air outlet and the second air outlet are communicated with the outside atmosphere; the first air outlet is communicated with the second air inlet through a first connecting pipeline, and a first switch valve is connected to the first connecting pipeline; a second connecting pipeline is communicated with the first connecting pipeline, the tail end of the second connecting pipeline is connected with a third air inlet, and a second switch valve is connected to the second connecting pipeline; the first switch valve is positioned between the second connecting pipeline and the second aerosol collecting box; the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all connected with a gas sampling device; beehives filled with sick bee colonies are placed in the first aerosol collecting box, and beehives filled with healthy bee colonies are placed in the second aerosol collecting box and the third aerosol collecting box.
2. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: the gas sampling device comprises a gas sampling bottle and a vacuum pump, wherein a gas inlet pipe of the gas sampling bottle is communicated with the aerosol collection box, and a gas outlet pipe of the gas sampling bottle is connected with a gas inlet end of the vacuum pump; phosphate buffer saline solution is filled in the gas sampling bottle.
3. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: the first air inlet is connected with the positive pressure fan.
4. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: a beehive filled with healthy bee colonies is also placed in the first aerosol collection box.
5. The experimental apparatus for collecting bio-aerosol of beehive according to claim 2, wherein: the air filtering mechanism is a glass fiber filter membrane.
6. The experimental apparatus for collecting bio-aerosol of beehive according to claim 1, wherein: and the first aerosol collecting box, the second aerosol collecting box and the third aerosol collecting box are all provided with box doors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010458641.7A CN111487095A (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010458641.7A CN111487095A (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111487095A true CN111487095A (en) | 2020-08-04 |
Family
ID=71795320
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010458641.7A Pending CN111487095A (en) | 2020-05-27 | 2020-05-27 | Experimental apparatus is gathered to beehive bioaerosol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111487095A (en) |
-
2020
- 2020-05-27 CN CN202010458641.7A patent/CN111487095A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Haig et al. | Bioaerosol sampling: sampling mechanisms, bioefficiency and field studies | |
| Thorne et al. | Comparison of bioaerosol sampling methods in barns housing swine | |
| US6782845B1 (en) | IVC rack system and method for detecting infections particles within an IVC rack system | |
| CN206131408U (en) | Ventilated sterilization device for farm | |
| Haas et al. | Comparative study of impaction and sedimentation in an aerosol chamber using defined fungal spore and bacterial concentrations | |
| Wenke et al. | Efficiency of different air filter types for pig facilities at laboratory scale | |
| CN212159256U (en) | Experimental apparatus is gathered to beehive bioaerosol | |
| CN202744553U (en) | Mycoplasma hyopneumoniae aerosol sampler | |
| CN111487095A (en) | Experimental apparatus is gathered to beehive bioaerosol | |
| KR101694895B1 (en) | Method for simultaneous sampling and quantification of bacteria and host-based virus | |
| CN108332299A (en) | A kind of laboratory ion cleaning module | |
| CN102101062B (en) | Aerosol microenvironment enclosed cabin for aerosol experiment | |
| CN214309773U (en) | Hierarchical sampling device of cavy expiratory aerosol | |
| CN202757231U (en) | Air purifier | |
| CN201912942U (en) | Aerosol microenvironment sealed cabin for aerosol experiment | |
| Nguyen et al. | Evaluation of Bioaerosol Samplers for Airborne Escherichia coli Carried by Poultry Litter Particles | |
| RU2493258C1 (en) | Method to detect number of microorganisms in air | |
| Graetz et al. | Spray mist and aerosol control in dental room air–summary of current evidence | |
| CN108982171A (en) | Gas virus collection method and gas utilization device | |
| Knight | Bioaerosols in agriculture: quantifying total airborne bacteria concentrations using molecular biology tools | |
| CN107907574B (en) | Experimental method for tea geometrid living body detection | |
| Wu et al. | Study on the Scavenging Effect of Steady-state Displacement Air Purification System on Indoor Bioaerosol | |
| Fiorina et al. | A portable sampler (PARTRAP FA 52) for microbiological evaluation of airborne particles: comparison with standard sedimetric and volumetric methods in haemodialysis rooms | |
| KR101597517B1 (en) | Method for Host-based virus Sampling by Impaction Air Sampler | |
| CN104197415B (en) | A kind of Animal Lab. air conditioning and cleaning system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |