CN104224925B - A kind of mosquito-expelling and antipruritic Chinese medicine composition and application thereof - Google Patents
A kind of mosquito-expelling and antipruritic Chinese medicine composition and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of mosquito-expelling and antipruritic Chinese medicine composition and application thereof, said composition is made up of following ingredients: eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil, seed of bark of eucommia extract and substrate；Wherein eucalyptus citriodora quintessence oil is 10%～50%, and Fructus Cnidii quintessence oil is 3%～10%, and Herba Origani oil is 0.5%～5%, and it is 0.2%～3.0% that seed of bark of eucommia extract accounts for the weight ratio of said composition；Above percentage ratio is all weight percentage.The mosquito-expelling and antipruritic Chinese medicine composition of the present invention has preferably mosquito-expelling and antipruritic effect.
Description
Technical field
The present invention is specifically related to a kind of mosquito-expelling and antipruritic Chinese medicine composition and application thereof.
Background technology
The Dulicidae of mosquito generic segmental appendage class animal door Insecta Diptera Nematocera, humans and animals not only can be caused suction by it
Blood is harassed, and is present in the anaphylactogen in its saliva and can induce human body immediate hypersensitivity, and such as the welt of pruritus, or delayed surpasses
Quick reaction, such as hardening pimple, it or the communication media of multiple insect borne disease, arthropod borne disease in addition.
People knows from experience by breathing and the skin metabolism substantial amounts of metabolite of generation, including carbon dioxide and the secretion of skin body of gland
Thing, is changed into all kinds of volatile matter odorous under the effect of these tasteless secretions microorganisms on skin, including carboxylic acids,
Alcohol, aldehyde, ketone, aromatic hydrocarbon, amide etc..These chemical substances constantly discharge in surrounding, and it is required to provide to mosquito
Environmental information, female mosquito just carries out host location by these volatile chemical substances.Gu Yilu etc. [1] are to being distributed in human body skin
The attracting action of mosquito is studied by several chemokineses on skin surface, result display carbamide, lactic acid, hexadecanoic acid and nonyl
Mosquito is had by aldehyde etc. higher quotes power.In addition research shows that the allure of mosquito is all significantly stronger than single by abnormal smells from the patient mixture
Abnormal smells from the patient thing, such as [2] such as lactic acid, carbon dioxide, ammonia, it can thus be appreciated that mosquito location host is human body different odor material synergism
Result.
Repellant is that one lures inhibitor, and it mainly suppresses target to be just present in all kinds of volatility gas that human body shows
Taste molecule, including lactic acid, carbon dioxide etc., reduces the Olfactory receptor neurons sensitivity to various attractants, to weaken mosquito
Perception to host-odor, upsets it and host is found behavior.As a example by DEET with matsutake alcohol (Octenol), DEET can be with pungent
Enol combines thus changes the volatile chemical composition of skin surface thus reduce the human body skin captivation to mosquito.
DEET (diethyltoluamide；N, N Diethyl-m-toluamide) it is a kind of one developed by the scientist of United States Department of Agriculture (USDA)
Broad spectrum insect repellent, the smell is awful, stimulates skin, and has certain Nervous toxicity side effect.On market, mosquito repellent product is general
Containing DEET class chemosynthesis composition.
The most conventional many employings of pure natural mosquito-repellent product have good market cognition degree and the plant of clear and definite efficacy effect
Composition, how based on volatile ingredient, as citronella oil, Flos Pelargonii, Herba Rosmarini Officinalis, lemon grass (Cymbopogon citratus) etc..But it is each more than in many series products
Frequently using of kind of composition, makes effect distinctiveness between product inconspicuous, lacks novelty.It addition, some pure natural mosquito-repellents
Series products, mosquito repellent function is more weak.
Pruritus is the offending sensation of a kind of skin mucosa causing desire of scratching.Owing to infecting, being dried or other skins
Skin damage (such as mosquito bite) causes, and all may result in skin pruritus.Under pathological conditions, keratinocyte can with secretory cell because of
The multiple endogenous such as son, amine, neuropeptide, nerve growth factor, opioids and eicosanoid causes to itch material, stimulates loose thin
Born of the same parents discharge histamine or direct sensitization skin C-nerve fiber sensor induction skin pruritus.
External treatment with Chinese medicine pruritus multiselect with having expelling wind and removing dampness, removing toxic substances parasite killing, nourshing blood and promoting blood circulation, removing heat from blood moisturize the medicine of effect,
The most frequently used has Radix Sophorae Flavescentis, Cortex Phellodendri, the Fructus Kochiae, Fructus Cnidii, Cortex Dictamni, Pericarpium Zanthoxyli, Fructus Xanthii, Radix Saposhnikoviae, Periostracum Cicadae, Radix Angelicae Sinensis, Herba Menthae.
Fructus Cnidii (Cnidium monnieri), for the dry mature fruit of samphire cnidium monnieri.Fructus Cnidii is warm in nature, taste
Pungent, bitter.There is warming the kidney to invigorate YANG, dampness, dispel the wind, effect of parasite killing.It is usually used in sexual impotence, cold womb, cold-damp leukorrhagia, arthralgia chiefly caused by damp pathogen lumbago；Outward
Control vulval eczema, married woman's pudendal pruritus, trichomonal vaginitis.Fructus Cnidii main composition is that isoimperatorin and osthole etc. are little
Polarity Coumarins composition, is not suitable for water extraction, general organic solvent such as the ethyl acetate that uses, petroleum ether, ethanol etc. or employing
Supercritical extraction mode, fully extracts Coumarins active component.
Adeps Bovis seu Bubali (Origanum vulgare), has another name called dysentery relieving grass, Herba Origani, Herba Ziziphorae clinopodioidis, for Labiatae Origanum for many years
Raw herbaceous plant.Be distributed mainly on Mediterranean Region to the Central Asia, north African, North America and China's most area.Adeps Bovis seu Bubali acrid in the mouth, property
Cool, nontoxic, herb can be used as medicine, and has clearing away heat and expelling pathogen in the exterior, regulating QI to expel dampness and urinates effect of detumescence.Adeps Bovis seu Bubali contains more than 30 kind of antibacterial compounds
Thing.Adeps Bovis seu Bubali rich in little polarity derived essential oils such as carvacrol, unsuitable water extraction,
Eucalyptus citriodora quintessence oil is Myrtaceae Myrtaceae eucalyptus plant (Eucalyptus) Eucalyptuscitriodoraoil tree
The quintessence oil extracted in leaf, uses water vapour distillation more, or is prepared by CO2 process for super-critical extracting.Eucalyptus citriodora quintessence oil contains
The aromatics compositions such as abundant citronellal, glycol in the Meng.
Seed of bark of eucommia is the seed of Eucommia, seed-producing rate about 2-3%, Eucommia seed oil content typically 27%～
33%, rich in polyunsaturated fatty acid in seed of bark of eucommia extract.Seed of bark of eucommia extract, containing abundant aucubin, has antioxygen
The effects such as change, clearing away damp-heat, antibacterial, antiinflammatory.
Aucubin structural formula is as follows
Summary of the invention
The technical problem to be solved there is provided a kind of and prior art diverse mosquito-expelling and antipruritic Chinese medicine
Compositions.The mosquito-expelling and antipruritic Chinese medicine composition of the present invention has preferably mosquito-expelling and antipruritic effect.
The invention provides a kind of mosquito-expelling and antipruritic Chinese medicine composition, it is made up of following ingredients: eucalyptus citriodora quintessence oil, Fructus Cnidii
Quintessence oil, Herba Origani oil, seed of bark of eucommia extract and substrate；Wherein eucalyptus citriodora quintessence oil is 10%～50%, Fructus Cnidii quintessence oil be 3%～
10%, Herba Origani oil is 0.5%～5%；It is 0.2%～3.0% that seed of bark of eucommia extract accounts for the weight ratio of said composition, and surplus is base
Matter, above percentage ratio is all weight percentage；Described substrate is water, glycerol or propylene glycol.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, preferably: described eucalyptus citriodora quintessence oil be 20%～
50%, described Fructus Cnidii quintessence oil is 5%～10%, and described Herba Origani oil is 1%～5%；Described seed of bark of eucommia extract accounts for
The weight ratio of said composition is 0.5%～3.0%, and above percentage ratio is all weight percentage.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, more preferably: described eucalyptus citriodora quintessence oil be 30%～
50%, described Fructus Cnidii quintessence oil is 7%～10%, and described Herba Origani oil is 2%～5%；Described seed of bark of eucommia extract accounts for
The weight ratio of said composition is 1.0%～3.0%, and above percentage ratio is all weight percentage.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, optimal: described eucalyptus citriodora quintessence oil is 50%, institute
The Fructus Cnidii quintessence oil stated is 10%, and described Herba Origani oil is 5%；Described seed of bark of eucommia extract accounts for the weight ratio of said composition
3.0%, above percentage ratio is all weight percentage.
In the present invention, described eucalyptus citriodora quintessence oil is the eucalyptus citriodora quintessence oil of this area routine or passes through to be commercially available, typically
Can be prepared by following method: by Folium Eucalypti Citriodorae extraction by steam distillation, or CO2Supercritical extraction, to obtain final product.
In the present invention, described Fructus Cnidii quintessence oil is the Fructus Cnidii quintessence oil of this area routine or passes through to be commercially available, typically
Can be prepared by following method: after Fructus Cnidii is fully pulverized, use CO2Supercritical extraction, to obtain final product；Or by abundant for Fructus Cnidii powder
After broken, by ethanol, ethanol water or ethyl acetate 20～35 DEG C of extractions, reclaim organic solvent, to obtain final product.
In the present invention, described Herba Origani oil is the Herba Origani oil of this area routine or passes through to be commercially available, Herba Origani oil
(Oregano oil) is a kind of volatile essential oils extracted from plant Adeps Bovis seu Bubali, and its main component has carvacrol, Herba thymi vulgaris
Quinone, dipentene, coriandrol and carvacrol etc. in phenol, thymol, stone.Typically can be prepared by following method: Adeps Bovis seu Bubali is used
CO2Supercritical extraction and get final product, or by Adeps Bovis seu Bubali ethyl acetate, petroleum ether or ethanol 20～35 DEG C of extractions, reclaim acetic acid second
Ester, petroleum ether or ethanol, to obtain final product.
In the present invention, described seed of bark of eucommia extract is the seed of bark of eucommia extract of this area routine or passes through to be commercially available.
Rich in multiple unsaturated fatty acid in seed of bark of eucommia extract, wherein alpha-linolenic acid accounts for more than the 60% of fatty acid, containing abundant Fructus Persicae
Leaf Corallium Japonicum Kishinouye glycosides.Typically prepared by following method: seed of bark of eucommia dregs of rice ethyl acetate is extracted liposoluble constituent, fat-soluble one-tenth will be removed
The hot water extraction that residue temperature is 60～80 DEG C divided, decolouring, concentrate.
In the present invention, each composition is simply mixed, above-mentioned mosquito-expelling and antipruritic compositions.
Present invention also offers the application in preparing mosquito-expelling and antipruritic medicine of the above-mentioned mosquito-expelling and antipruritic Chinese medicine composition.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can combination in any, obtain the present invention each preferably
Example.
Agents useful for same of the present invention and raw material are the most commercially.
The most progressive effect of the present invention is: the mosquito-expelling and antipruritic Chinese medicine composition of the present invention has preferably mosquito-expelling and antipruritic
Effect.
Detailed description of the invention
Further illustrate the present invention below by the mode of embodiment, but the most therefore limit the present invention to described reality
Execute among example scope.The experimental technique of unreceipted actual conditions in the following example, conventionally and condition, or according to business
Product description selects.
" % " in following each embodiment if no special instructions, refers both to percentage by weight.
Preparation embodiment 1～16
Embodiment 1
Take 10Kg Folium Eucalypti Citriodorae, with extraction by steam distillation 4 hours, extract eucalyptus citriodora oil phase volatile oil, obtain 500g lemon
Lemon eucalyptus globulus quintessence oil.
Take 10Kg Fructus Cnidii, after fully pulverizing, cross 40 mesh sieves, be placed in supercritical carbon dioxide extraction instrument, with titanium dioxide
It is respectively 5.2MPa and 30 that carbon is respectively 25MPa and 50 DEG C, separating pressure and separation temperature in extracting pressure and extraction temperature
Under conditions of DEG C, extract 6 hours, collect extract, obtain 100g Fructus Cnidii quintessence oil.
Take 2500g Adeps Bovis seu Bubali medical material, pulverize, cross 20 mesh sieves, put in supercritical carbon dioxide extraction instrument, with carbon dioxide in extraction
Pressure power and extraction temperature are respectively 15MPa and 35 DEG C, and separating pressure and separation temperature are respectively 5.2MPa and the bar of 30 DEG C
Under part, extract 1 hour, collect extract 22.8g.By extract 20g, add the ethanol of 8-10 times amount (ml/g) 80% (v/v),
Mix homogeneously, places 12 hours, puts in centrifuge in more than 5000 turns centrifugation 30 minutes, pours out supernatant, and residue adds 4 times
Amount (ml/g) with concentration washing with alcohol once, centrifugal under conditions of same, collect supernatant, supernatant merged, reclaim
Ethanol, to only, collects 12.8g Herba Origani oil.
Weigh the 150g seed of bark of eucommia dregs of rice, grind, be placed in the there-necked flask of 1000mL, add 600mL petroleum ether, the seed of bark of eucommia dregs of rice
Forming solid-liquid ratio with petroleum ether is the mixed liquor of 1:4g/mL, is heated to 45 DEG C and extracts 2 hours, repeat to extract 3 times, removes and extract
Liquid, adds the ethyl acetate of 600mL in residue, and the seed of bark of eucommia dregs of rice are 1:4g/mL with the solid-liquid ratio of ethyl acetate, at 45 DEG C
React 2 hours, be repeated 3 times, remove extracting solution, reclaim ethyl acetate solvent, obtain removing the seed of bark of eucommia dregs of rice of liposoluble constituent.Will
The seed of bark of eucommia dregs of rice removing liposoluble constituent are placed in there-necked flask, add the hot water extraction 2 hours that 600mL temperature is 70 DEG C, weight
Extracting 2 times again, the seed of bark of eucommia dregs of rice are 1:4g/mL with the solid-liquid ratio of hot water, reclaim extracting solution, process with activated carbon decolorizing ,-
0.093MPa, 45 DEG C of concentrating under reduced pressure, obtain 30g seed of bark of eucommia extract.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 0.2%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound,.
Embodiment 2
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are and are commercially available.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 0.2%, surplus is glycerol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic
Compositions.
Embodiment 3
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 0.5%；Seed of bark of eucommia extract accounts for this combination
The weight ratio of thing is 0.2%, and surplus is propylene glycol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain anophelifuge
Anti-pruritic compositions.
Embodiment 4
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 0.2%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 5
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 3.0%, and surplus is glycerol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Embodiment 6
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 1%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 0.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 7
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 1%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 0.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 8
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 1%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 3.0%, surplus is propylene glycol, and said composition gross weight is 500g.By each composition mix homogeneously, only obtain anophelifuge
Itch compositions.
Embodiment 9
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 1%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 3.0%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Embodiment 10
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 2.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 11
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 7%, and Herba Origani oil is 2%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 1.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 12
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 7%, and Herba Origani oil is 2%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 1.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 13
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 1.0%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Embodiment 14
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 40%, and Fructus Cnidii quintessence oil is 8%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 2.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Embodiment 15
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 40%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 2%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 3.0%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Embodiment 16
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 50%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 3.0%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Embodiment 17
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 50%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%；Seed of bark of eucommia extract accounts for said composition
Weight ratio be 3.0%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic group
Compound.
Comparative example 1～5
Comparative example 1
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%；, surplus is water, and said composition gross weight is 500g.By each composition
Mix homogeneously, obtains mosquito-expelling and antipruritic compositions.
Comparative example 2
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all prepared according to the method in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%；Seed of bark of eucommia extract accounts for the weight ratio of said composition
0.2%, surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 3
The source of eucalyptus citriodora quintessence oil, Herba Origani oil, Fructus Cnidii quintessence oil and seed of bark of eucommia extract is with embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 5%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 6%；Seed of bark of eucommia extract accounts for said composition
Weight ratio is 2.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic combination
Thing.
Comparative example 4
The source of eucalyptus citriodora quintessence oil, Herba Origani oil and seed of bark of eucommia extract is with embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Herba Origani oil is 0.5%；It is 0.2% that seed of bark of eucommia extract accounts for the weight ratio of said composition,
Surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 5
The source of eucalyptus citriodora quintessence oil and Fructus Cnidii quintessence oil is with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%,；Surplus is water, and said composition gross weight is 500g.Will be each
Composition mix homogeneously, obtains mosquito-expelling and antipruritic compositions.
Effect example
Effect example 1 anophelifuge is tested
Test method reference: GB/T13917.9-2009, agriculture chemical registration hygienic insecticide indoor pharmacodynamic test and evaluation
9th part: repellant.By different formulations mosquito-expelling and antipruritic product, to Aedes albopictus Efficacy experiments.
Each Example formulations product testing result such as following table:
Test shows, the mosquito-expelling and antipruritic Chinese medicine composition of the present invention has significant mosquito-repelling function.
The antipruritic test of effect example 2
Test method: 14-AP induction mouse skin pruritus model is set up
Kunming mouse, is randomly divided into 7 groups, often group 10, it may be assumed that normal group, model group, fluocinolone acetonide group (positive group), 4
Different formulations product group.Each group is all dissolved with vegetable oil or DMSO.Shaving about 2cm × 2cm in 1d back before medication, administration group is by medicine
Uniformly being applied on shaving district skin, normal group is coated with equivalent solvent vegetable oil with model group.Model group and administration group are at coating 30min
After at shaving subcutaneous injection 4-AP 1mg/kg, normal group injecting normal saline, record each group of Licking response respectively at 10min
In number of times (to lick body continuously to minibreak occurs, lick 1 calculating of body).
Antipruritic result of the test:
*: compare with model group, P < 0.01,△: compare with normal group, P < 0.05
Test shows, the mosquito-expelling and antipruritic Chinese medicine composition of the present invention has significant itching-relieving action.
The antipruritic experiment of effect example 3
Test method: with reference to 03 edition " study of tcm new drug guide "
Test material:
1, animal: Cavia porcellus 50 (often group 10) body weight: about 300g is female/and male half and half
2, histamine phosphate: Nat'l Pharmaceutical & Biological Products Control Institute's lot number: 0100319
3, positive control: positive Pharmaceutical 120701# of dexamethasone (25g:15mg) health
Experimental condition: laboratory temperature: 20 25 DEG C of relative humiditys: 60 70%
Test method: Cavia porcellus is randomly divided into 5 groups, often group 10.Divide blank group, positive controls, sample oil reservoir
Three groups.Test the previous day, to instep behind each group of Cavia porcellus right side, be coated with sample once.Experimental day, behind the coarse sandpaper scratch animal right side
At the shaving of instep, about about 1 square centimeter of scope, then smearing sample in this place once, blank group gives equivalent distilled water.
After 10 minutes, start at wound face, only to drip 0.01% histamine phosphate 0.05ml/, hereafter every 3 minutes according to 0.01%,
0.02%, 0.03%, 0.04%...... progressive concentration, only it is 0.05ml/ every time.Until after occurring that Cavia porcellus later licks the right side
, finally to there is histamine phosphate total amount that Cavia porcellus given when later licking right metapedes as itch-threshold in foot.Record relatively respectively group
Itch-threshold.
Result of the test:
Each embodiment prepares product and histamine phosphate is caused the impact reacted of itching
*: P value is test group and compares with blank group
Evaluation of result:
With blank group, the mosquito-expelling and antipruritic compositions that embodiment 1, embodiment 6 and embodiment 16 prepare all can improve globefish
Mus itch-threshold effect (P < 0.01).
The acute toxicity test of the mosquito-expelling and antipruritic compositions of effect example 4 present invention
1 material and method
1.1 samples: the mosquito-expelling and antipruritic compositions that various embodiments of the present invention prepare.
1.2 animals: Kunming kind healthy adult mice 60, male and female half and half, are carried by Kunming Medical University's Experimental Animal Center
Confession, male and female half and half, weight range: 22-26g, it is randomly divided into 3 groups, through mouth gavage after fasting 10-11 hour.
1.3 dosage are arranged: first group of mouse stomach concentration is: the product that embodiment 1 prepares is (by kg body weight conversion for living
Property composition: 5g/kg.bw), solvent is vegetable oil, disposably gives；Second group of mouse stomach concentration is the mixture of embodiment 11
(being active component: 4g/kg.bw by kg body weight conversion), solvent is vegetable oil, disposably gives；3rd group of mouse stomach is dense
The mixture (being active component: 3g/kg.bw by kg body weight conversion) that degree is embodiment 16, solvent is vegetable oil, disposably gives
Give；Close observation 4 hours after three groups of gavages, conventional raising 14 days.
2 results
First group of its mouse oral acute toxicity result
Second group of its mouse oral acute toxicity result
3rd group to its mouse oral acute toxicity result
3 conclusions
The mosquito-expelling and antipruritic compositions toxicity test result of the present invention
Mouse growth growth, food-intake, activity etc. are the most without exception, without dead, show maximum tolerated dose be more than 2g/kg without
Acute Toxicity.
Claims (8)
1. a mosquito-expelling and antipruritic Chinese medicine composition, it is characterised in that: it is made up of following ingredients: eucalyptus citriodora quintessence oil, Fructus Cnidii essence
Oil, Herba Origani oil, seed of bark of eucommia extract and substrate；Wherein eucalyptus citriodora quintessence oil is 10%～50%, Fructus Cnidii quintessence oil be 3%～
10%, Herba Origani oil is 0.5%～5%；It is 0.2%～3.0% that seed of bark of eucommia extract accounts for the weight ratio of said composition, and surplus is base
Matter；Above percentage ratio is all weight percentage；Described substrate is water, glycerol or propylene glycol.
2. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 1, it is characterised in that: described eucalyptus citriodora quintessence oil be 20%～
50%, described Fructus Cnidii quintessence oil is 5%～10%, and described Herba Origani oil is 1%～5%；Described seed of bark of eucommia extract accounts for
The weight ratio of said composition is 0.5%～3.0%, and above percentage ratio is all weight percentage.
3. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 2, it is characterised in that: described eucalyptus citriodora quintessence oil be 30%～
50%, described Fructus Cnidii quintessence oil is 7%～10%, and described Herba Origani oil is 2%～5%；Described seed of bark of eucommia extract accounts for
The weight ratio of said composition is 1.0%～3.0%, and above percentage ratio is all weight percentage.
4. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 3, it is characterised in that: described eucalyptus citriodora quintessence oil is 50%,
Described Fructus Cnidii quintessence oil is 10%, and described Herba Origani oil is 5%；Described seed of bark of eucommia extract accounts for the weight ratio of said composition
Being 3.0%, above percentage ratio is all weight percentage.
5. the mosquito-expelling and antipruritic Chinese medicine composition as described in any one of Claims 1 to 4, it is characterised in that: described eucalyptus citriodora essence
Oil is prepared by following method: by Folium Eucalypti Citriodorae extraction by steam distillation, or CO2Supercritical extraction, to obtain final product.
6. the mosquito-expelling and antipruritic Chinese medicine composition as described in any one of Claims 1 to 4, it is characterised in that: described Fructus Cnidii essence
Oil is prepared by following method: after fully being pulverized by Fructus Cnidii, uses CO2Supercritical extraction, to obtain final product；Or by abundant for Fructus Cnidii powder
After broken, by ethanol, ethanol water or ethyl acetate 20～35 DEG C of extractions, reclaim organic solvent, to obtain final product.
7. the mosquito-expelling and antipruritic Chinese medicine composition as described in any one of Claims 1 to 4, it is characterised in that: described Herba Origani oil by
Following method prepares: by Adeps Bovis seu Bubali CO2Supercritical extraction and get final product, or by Adeps Bovis seu Bubali ethyl acetate, petroleum ether or ethanol 20
～35 DEG C of extractions, reclaim ethyl acetate, petroleum ether or ethanol, to obtain final product.
8. the application in preparing mosquito-expelling and antipruritic medicine of the mosquito-expelling and antipruritic Chinese medicine composition as described in any one of claim 1～7.
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| CN106214745A (en) * | 2016-08-31 | 2016-12-14 | 防城港市防城区那梭香料厂 | A kind of compound recipe Oleum Cinnamomi nano-emulsion and preparation method thereof |
| CN110179693B (en) * | 2019-06-14 | 2022-09-16 | 江西中医药大学 | Mosquito-repelling itching-relieving compound essential oil, preparation method and application thereof, and mosquito-repelling itching-relieving article |
| CN110755346A (en) * | 2019-10-31 | 2020-02-07 | 洪雅县瓦屋山药业有限公司 | Mosquito-repelling and itching-relieving Coptis deltoidea toilet water and preparation method thereof |
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