CN104224925A - Mosquito repellent and itching relieving traditional Chinese medicine composition and application thereof - Google Patents
Mosquito repellent and itching relieving traditional Chinese medicine composition and application thereof Download PDFInfo
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Abstract
The invention discloses a mosquito repellent and itching relieving traditional Chinese medicine composition and application thereof. The composition is composed, by weight, of 10%-50%, of lemon eucalyptus essential oil, 3%-10% of fructus cnidii essential oil, 0.5%-5% of oregano oil and 0.2%-3.0% of eucommia seed extract. The mosquito repellent and itching relieving traditional Chinese medicine composition has good mosquito repellent and itching relieving effects.
Description
Technical field
The present invention is specifically related to a kind of mosquito-expelling and antipruritic Chinese medicine composition and application thereof.
Background technology
The Dulicidae of mosquito generic segmental appendage class animal door Insecta Diptera Nematocera, it not only can cause to humans and animals harassing and wrecking of sucking blood, the anaphylactogen be present in its saliva can bring out human body immediate hypersensitivity, as the welt of pruritus, or delayed hypersensitivity, as hardening pimple, it or the communication media of multiple insect borne disease, arthropod borne disease in addition.
People's cognition produces a large amount of metabolite by breathing and skin metabolism, comprise the secretions of carbon dioxide and skin body of gland, change all kinds of volatile matter odorous under the effect of these microorganisms of tasteless secretions on skin, comprise carboxylic acids, alcohol, aldehyde, ketone, aromatic hydrocarbon, amide etc.Discharge in the continuous environment towards periphery of these chemical substances, provide required environmental information to mosquito, female mosquito just carries out host location by these volatile chemical substances.Gu Yilu etc. [1] are studied the attracting action of several chemokineses to mosquito being distributed in human skin, and result display carbamide, lactic acid, hexadecanoic acid and aldehyde C-9 etc. have mosquito higher quotes power.In addition research shows that abnormal smells from the patient mixture is all significantly better than single abnormal smells from the patient thing to the allure of mosquito, as [2] such as lactic acid, carbon dioxide, ammonia, it can thus be appreciated that mosquito location host is the synergistic result of human body different odor material.
Repellant is that one lures inhibitor, it mainly suppresses target to be exactly be present in all kinds of volatile flavor molecules that human body shows, comprise lactic acid, carbon dioxide etc., reduce Olfactory receptor neurons to the sensitivity of various attractant, to weaken the perception of mosquito to host-odor, upset it and behavior is found to host.For DEET and matsutake alcohol (Octenol), DEET can be combined with matsutake alcohol thus changes the volatile chemical composition of skin surface thus reduce the captivation of human body skin to mosquito.DEET (diethyltoluamide; N, N-Diethyl-m-toluamide) be a kind of a kind of broad spectrum insect repellent developed by the scientist of United States Department of Agriculture (USDA), the smell is awful, chafe, and have certain Nervous toxicity side effect.On market, mosquito repellent product is generally containing DEET class chemosynthesis composition.
The at present conventional many employings of pure natural mosquito-repellent product have the plant component of good market cognition degree and clear and definite efficacy effect, are main mainly with volatile ingredient, as citronella oil, Flos Pelargonii, Herba Rosmarini Officinalis, lemon grass (Cymbopogon citratus) etc.But the frequent use of various composition more than in multiclass product, makes effect distinctiveness between product and not obvious, lacks novelty.In addition, some pure natural mosquito-repellent series products, mosquito repellent function is more weak.
Pruritus is a kind of offending sensation causing the skin mucosa of desire of scratching.Because infection, drying or other skin injurys (as mosquito bite) cause, all skin pruritus can be caused.Under pathological conditions, keratinocyte can cause material of itching by the multiple endogenous such as secrete cytokines, amine, neuropeptide, nerve growth factor, opioids and eicosanoid, stimulate histamine release of mast cell or directly sensitization skin C-nerve fiber sensor bring out skin pruritus.
The external treatment with Chinese medicine pruritus multiselect medicine having expelling wind and removing dampness, removing toxic substances parasite killing, nourshing blood and promoting blood circulation, removing heat from blood moisturize effect, the most frequently used has Radix Sophorae Flavescentis, Cortex Phellodendri, the Fructus Kochiae, Fructus Cnidii, Cortex Dictamni, Pericarpium Zanthoxyli, Fructus Xanthii, Radix Saposhnikoviae, Periostracum Cicadae, Radix Angelicae Sinensis, Herba Menthae.
Fructus Cnidii (Cnidium monnieri) is the dry mature fruit of samphire cnidium monnieri.Fructus Cnidii is warm in nature, acrid in the mouth, hardship.There is warming the kidney to invigorate YANG, dampness, dispel the wind, effect of parasite killing.Be usually used in sexual impotence, cold womb, cold-damp leukorrhagia, arthralgia chiefly caused by damp pathogen lumbago; External treatment vulval eczema, married woman's pudendal pruritus, trichomonal vaginitis.Fructus Cnidii main composition is the little polarity Coumarins such as isoimperatorin and osthole composition, be not suitable for water extraction, generally adopt organic solvent as ethyl acetate, petroleum ether, ethanol etc. or employing supercritical extraction mode, fully extract Coumarins active component.
Adeps Bovis seu Bubali (Origanum vulgare), has another name called dysentery relieving grass, Herba Origani, Herba Ziziphorae clinopodioidis, is Labiatae Origanum herbaceos perennial.Mainly be distributed in Mediterranean Region to the Central Asia, north African, North America and China's most area.Adeps Bovis seu Bubali acrid in the mouth, cool in nature, nontoxic, herb can be used as medicine, and has clearing away heat and expelling pathogen in the exterior, effect that regulating QI to expel dampness falls urine detumescence.Adeps Bovis seu Bubali contains more than 30 kind of antimicrobial compound.Adeps Bovis seu Bubali is rich in the little polarity derived essential oils such as carvacrol, unsuitable water extraction,
Eucalyptus citriodora quintessence oil is the quintessence oil extracted in Myrtaceae Myrtaceae eucalyptus plant (Eucalyptus) Eucalyptuscitriodoraoil leaves, adopts water vapour distillation more, or the preparation of CO2 process for super-critical extracting.In eucalyptus citriodora quintessence oil containing abundant citronellal, the Meng aromatics composition such as glycol.
Seed of bark of eucommia is the seed of Eucommia, and seed-producing rate is about 2-3%, and Eucommia seed oil content, generally 27% ~ 33%, is rich in polyunsaturated fatty acid in seed of bark of eucommia extract.Seed of bark of eucommia extract, containing enriching aucubin, has the effects such as antioxidation, clearing away damp-heat, antibacterial, antiinflammatory.
Aucubin structural formula is as follows
Summary of the invention
Technical problem to be solved by this invention there is provided a kind of and the diverse mosquito-expelling and antipruritic Chinese medicine composition of prior art.Mosquito-expelling and antipruritic Chinese medicine composition of the present invention has preferably mosquito-expelling and antipruritic effect.
The invention provides a kind of mosquito-expelling and antipruritic Chinese medicine composition, it is made up of following ingredients: eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil, seed of bark of eucommia extract and substrate; Wherein eucalyptus citriodora quintessence oil is 10% ~ 50%, and Fructus Cnidii quintessence oil is 3% ~ 10%, and Herba Origani oil is 0.5% ~ 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2% ~ 3.0%, and surplus is substrate, and above percentage ratio is all weight percentage; Described substrate is water, glycerol or propylene glycol.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, preferably: described eucalyptus citriodora quintessence oil is 20% ~ 50%, described Fructus Cnidii quintessence oil is 5% ~ 10%, and described Herba Origani oil is 1% ~ 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 0.5% ~ 3.0%, and above percentage ratio is all weight percentage.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, better: described eucalyptus citriodora quintessence oil is 30% ~ 50%, described Fructus Cnidii quintessence oil is 7% ~ 10%, and described Herba Origani oil is 2% ~ 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 1.0% ~ 3.0%, and above percentage ratio is all weight percentage.
In the present invention, in described mosquito-expelling and antipruritic Chinese medicine composition, best: described eucalyptus citriodora quintessence oil is 50%, and described Fructus Cnidii quintessence oil is 10%, and described Herba Origani oil is 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 3.0%, and above percentage ratio is all weight percentage.
In the present invention, described eucalyptus citriodora quintessence oil is the eucalyptus citriodora quintessence oil of this area routine or is obtained by commercially available, generally can be obtained by following method: by Folium Eucalypti Citriodorae extraction by steam distillation, or CO
2supercritical extraction, to obtain final product.
In the present invention, described Fructus Cnidii quintessence oil is the Fructus Cnidii quintessence oil of this area routine or is obtained by commercially available, generally can be obtained by following method: after fully being pulverized by Fructus Cnidii, adopts CO
2supercritical extraction, to obtain final product; Or after Fructus Cnidii is fully pulverized, by ethanol, ethanol water or ethyl acetate 20 ~ 35 DEG C of lixiviates, reclaim organic solvent, to obtain final product.
In the present invention, described Herba Origani oil is the Herba Origani oil of this area routine or is obtained by commercially available, Herba Origani oil (Oregano oil) is a kind of volatile essential oils extracted from plant Adeps Bovis seu Bubali, and its main component has quinone, dipentene, coriandrol and carvacrol etc. in carvacrol, thymol, thymol, stone.Generally can be obtained by following method: by Adeps Bovis seu Bubali CO
2supercritical extraction and get final product, or by Adeps Bovis seu Bubali ethyl acetate, petroleum ether or ethanol 20 ~ 35 DEG C of lixiviates, reclaim ethyl acetate, petroleum ether or ethanol, to obtain final product.
In the present invention, described seed of bark of eucommia extract is the seed of bark of eucommia extract of this area routine or is obtained by commercially available.Be rich in multiple unsaturated fatty acid in seed of bark of eucommia extract, wherein alpha-linolenic acid accounts for more than 60% of fatty acid, containing enriching aucubin.Generally being obtained by following method: seed of bark of eucommia dregs of rice ethyl acetate is extracted liposoluble constituent, is the hot water extraction of 60 ~ 80 DEG C by the residue temperature of removing liposoluble constituent, and decolouring is concentrated.
In the present invention, each composition is simply mixed, above-mentioned mosquito-expelling and antipruritic compositions.
Present invention also offers above-mentioned mosquito-expelling and antipruritic Chinese medicine composition and prepare the application in mosquito-expelling and antipruritic medicine.
Without prejudice to the field on the basis of common sense, above-mentioned each optimum condition, can combination in any, obtains the preferred embodiments of the invention.
Agents useful for same of the present invention and raw material are all commercially.
Positive progressive effect of the present invention is: mosquito-expelling and antipruritic Chinese medicine composition of the present invention has preferably mosquito-expelling and antipruritic effect.
Detailed description of the invention
Mode below by embodiment further illustrates the present invention, but does not therefore limit the present invention among described scope of embodiments.The experimental technique of unreceipted actual conditions in the following example, conventionally and condition, or selects according to catalogue.
" % " in following each embodiment if no special instructions, all refers to percentage by weight.
Preparation embodiment 1 ~ 16
Embodiment 1
Get 10Kg Folium Eucalypti Citriodorae, with extraction by steam distillation 4 hours, extract eucalyptus citriodora oil phase volatile oil, obtain 500g eucalyptus citriodora quintessence oil.
Get 10Kg Fructus Cnidii, after abundant pulverizing, cross 40 mesh sieves, be placed in supercritical carbon dioxide extraction instrument, be respectively 25MPa and 50 DEG C with carbon dioxide in extracting pressure and extraction temperature, separating pressure and separation temperature are under the condition being respectively 5.2MPa and 30 DEG C, extract 6 hours, collect extract, obtain 100g Fructus Cnidii quintessence oil.
Get 2500g Adeps Bovis seu Bubali medical material, pulverize, cross 20 mesh sieves, put in supercritical carbon dioxide extraction instrument, be respectively 15MPa and 35 DEG C with carbon dioxide in extracting pressure and extraction temperature, separating pressure and separation temperature are under the condition being respectively 5.2MPa and 30 DEG C, extract 1 hour, collect extract 22.8g.By extract 20g, add the ethanol of 8-10 times amount (ml/g) 80% (v/v), mix homogeneously, place 12 hours, put in centrifuge in more than 5000 turns centrifugation 30 minutes, incline and supernatant, residue add 4 times amount (ml/g) with concentration washing with alcohol once, centrifugal under same condition, collect supernatant, supernatant is merged, reclaims ethanol to clean, collect 12.8g Herba Origani oil.
Take the 150g seed of bark of eucommia dregs of rice, grind, be placed in the there-necked flask of 1000mL, add 600mL petroleum ether, the seed of bark of eucommia dregs of rice and petroleum ether form the mixed liquor that solid-liquid ratio is 1:4g/mL, are heated to 45 DEG C and extract 2 hours, repeat extraction 3 times, remove extracting solution, in residue, add the ethyl acetate of 600mL again, the solid-liquid ratio of the seed of bark of eucommia dregs of rice and ethyl acetate is 1:4g/mL, 45 DEG C of reactions 2 hours, repeat 3 times, remove extracting solution, reclaim ethyl acetate solvent, obtain the seed of bark of eucommia dregs of rice removing liposoluble constituent.The seed of bark of eucommia dregs of rice removing liposoluble constituent are placed in there-necked flask, add the hot water extraction 2 hours that 600mL temperature is 70 DEG C, repeat extraction 2 times, the solid-liquid ratio of the seed of bark of eucommia dregs of rice and hot water is 1:4g/mL, reclaim extracting solution, use activated carbon decolorizing process ,-0.093MPa, 45 DEG C of concentrating under reduced pressure, obtain 30g seed of bark of eucommia extract.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 2
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are commercially available obtaining.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is glycerol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 3
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 0.5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is propylene glycol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 4
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 5
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is glycerol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 6
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 1%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 7
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 1%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 8
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 1%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is propylene glycol, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 9
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 1%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 10
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 20%, and Fructus Cnidii quintessence oil is 5%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 2.5%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 11
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 7%, and Herba Origani oil is 2%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 1.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 12
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 7%, and Herba Origani oil is 2%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 1.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 13
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 30%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 1.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 14
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 40%, and Fructus Cnidii quintessence oil is 8%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 2.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 15
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 40%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 2%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 16
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 50%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Embodiment 17
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 50%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 3.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 1 ~ 5
Comparative example 1
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Fructus Cnidii quintessence oil is 3%, and Herba Origani oil is 0.5%; , surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 2
Eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil and seed of bark of eucommia extract are all according to the method preparation in embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 3
The source of eucalyptus citriodora quintessence oil, Herba Origani oil, Fructus Cnidii quintessence oil and seed of bark of eucommia extract is with embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 5%, and Fructus Cnidii quintessence oil is 10%, and Herba Origani oil is 6%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 2.0%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 4
The source of eucalyptus citriodora quintessence oil, Herba Origani oil and seed of bark of eucommia extract is with embodiment 1.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Herba Origani oil is 0.5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2%, and surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Comparative example 5
The source of eucalyptus citriodora quintessence oil and Fructus Cnidii quintessence oil is with embodiment 2.
The formula of mosquito-expelling and antipruritic Chinese medicine composition is as follows:
Eucalyptus citriodora quintessence oil is 10%, and Fructus Cnidii quintessence oil is 3%; Surplus is water, and said composition gross weight is 500g.By each composition mix homogeneously, obtain mosquito-expelling and antipruritic compositions.
Effect example
Effect example 1 anophelifuge is tested
Test method reference: GB/T13917.9-2009, agriculture chemical registration is with hygienic insecticide indoor pharmacodynamic test and evaluate the 9th part: repellant.By different formulations mosquito-expelling and antipruritic product, to Aedes albopictus Efficacy experiments.
Each Example formulations product testing result is as following table:
Test shows, mosquito-expelling and antipruritic Chinese medicine composition of the present invention has significant mosquito-repelling function.
The antipruritic test of effect example 2
Test method: 14-AP brings out mouse skin pruritus model and sets up
Kunming mouse, is divided into 7 groups at random, often organizes 10, that is: normal group, model group, fluocinolone acetonide group (positive group), 4 different formulations product groups.Each group all dissolves with vegetable oil or DMSO.Before medication, 1d shaves at back hair about 2cm × 2cm, and medicine is evenly applied to by administration group to be shaved on the skin of hair-fields, and normal group and model group are coated with equivalent solvent vegetable oil.Model group and administration group after coating 30min in shaving hair place subcutaneous injection 4-AP 1mg/kg, normal group injecting normal saline, record the number of times of each group of Licking response in 10min (licking body continuously to there is minibreak, licking body and calculating for 1 time) respectively.
Antipruritic result of the test:
*: compare with model group, P<0.01,
△: compare with normal group, P<0.05
Test shows, mosquito-expelling and antipruritic Chinese medicine composition of the present invention has significant itching-relieving action.
The antipruritic experiment of effect example 3
Test method: with reference to 03 edition " study of tcm new drug guide "
Test material:
1, animal: Cavia porcellus 50 (often organize 10) body weight: about 300g is female/and male half and half
2, histamine phosphate: Nat'l Pharmaceutical & Biological Products Control Institute's lot number: 0100319
3, positive control: the positive Pharmaceutical 120701# of dexamethasone (25g:15mg) health
Experimental condition: laboratory temperature: 20-25 DEG C of relative humiditys: 60-70%
Test method: Cavia porcellus is divided into 5 groups at random, often organizes 10.Divide blank group, positive controls, sample oil reservoir three groups.Test the previous day, to each group of right back instep of Cavia porcellus, be coated with sample once.Experimental day, shave hair place with the coarse sandpaper scratch right back instep of animal, about about 1 square centimeter of scope, then smear sample once at this place, blank group gives equivalent distilled water.After 10 minutes, start to drip 0.01% histamine phosphate 0.05ml/ at wound face place only, after this complied with 0.01%, 0.02%, 0.03%, 0.04%...... progressive concentration every 3 minutes, be only 0.05ml/ at every turn.Until occur that Cavia porcellus later licks right back foot, with finally occur Cavia porcellus later lick right back sufficient time the histamine phosphate total amount that gives for itch-threshold.Record and the itch-threshold of relatively more each group.
Result of the test:
Each embodiment obtains product causes reaction of itching impact on histamine phosphate
*: P value is test group and compares with blank group
Evaluation of result:
With blank group, the mosquito-expelling and antipruritic compositions that embodiment 1, embodiment 6 and embodiment 16 obtains all can improve Cavia porcellus itch-threshold effect (P<0.01).
The acute toxicity test of effect example 4 mosquito-expelling and antipruritic compositions of the present invention
1 materials and methods
1.1 samples: the mosquito-expelling and antipruritic compositions that various embodiments of the present invention are obtained.
1.2 animals: Kunming kind healthy adult mice 60, male and female half and half, are provided by Kunming Medical University's Experimental Animal Center, male and female half and half, and weight range: 22-26g is divided into 3 groups at random, through mouth gavage after fasting 10-11 hour.
1.3 dosage are arranged: first group of mouse stomach concentration is: the obtained product of embodiment 1 (be active component: 5g/kg.bw by kg body weight conversion), and solvent is vegetable oil, disposablely give; Second group of mouse stomach concentration is the mixture (be active component: 4g/kg.bw by kg body weight conversion) of embodiment 11, and solvent is vegetable oil, disposablely gives; 3rd group of mouse stomach concentration is the mixture (be active component: 3g/kg.bw by kg body weight conversion) of embodiment 16, and solvent is vegetable oil, disposablely gives; Close observation 4 hours after three groups of gavages, conventional raising 14 days.
2 results
First group of its mouse oral acute toxicity result
Second group of its mouse oral acute toxicity result
3rd group to its mouse oral acute toxicity result
3 conclusions
Mosquito-expelling and antipruritic compositions toxicity test result of the present invention
Mouse growth growth, food-intake, activity etc. are all without exception, without dead, show that maximum tolerated dose is greater than 2g/kg without Acute Toxicity.
Claims (8)
1. a mosquito-expelling and antipruritic Chinese medicine composition, is characterized in that: it is made up of following ingredients: eucalyptus citriodora quintessence oil, Fructus Cnidii quintessence oil, Herba Origani oil, seed of bark of eucommia extract and substrate; Wherein eucalyptus citriodora quintessence oil is 10% ~ 50%, and Fructus Cnidii quintessence oil is 3% ~ 10%, and Herba Origani oil is 0.5% ~ 5%; The weight ratio that seed of bark of eucommia extract accounts for said composition is 0.2% ~ 3.0%, and surplus is substrate; Above percentage ratio is all weight percentage; Described substrate is water, glycerol or propylene glycol.
2. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 1, it is characterized in that: described eucalyptus citriodora quintessence oil is 20% ~ 50%, described Fructus Cnidii quintessence oil is 5% ~ 10%, and described Herba Origani oil is 1% ~ 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 0.5% ~ 3.0%, and above percentage ratio is all weight percentage.
3. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 2, it is characterized in that: described eucalyptus citriodora quintessence oil is 30% ~ 50%, described Fructus Cnidii quintessence oil is 7% ~ 10%, and described Herba Origani oil is 2% ~ 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 1.0% ~ 3.0%, and above percentage ratio is all weight percentage.
4. mosquito-expelling and antipruritic Chinese medicine composition as claimed in claim 3, it is characterized in that: described eucalyptus citriodora quintessence oil is 50%, described Fructus Cnidii quintessence oil is 10%, and described Herba Origani oil is 5%; The weight ratio that described seed of bark of eucommia extract accounts for said composition is 3.0%, and above percentage ratio is all weight percentage.
5. the mosquito-expelling and antipruritic Chinese medicine composition as described in Claims 1 to 4, is characterized in that: described eucalyptus citriodora quintessence oil is obtained by following method: by Folium Eucalypti Citriodorae extraction by steam distillation, or CO
2supercritical extraction, to obtain final product.
6. the mosquito-expelling and antipruritic Chinese medicine composition as described in Claims 1 to 4, is characterized in that: described Fructus Cnidii quintessence oil is obtained by following method: after fully being pulverized by Fructus Cnidii, adopts CO
2supercritical extraction, to obtain final product; Or after Fructus Cnidii is fully pulverized, by ethanol, ethanol water or ethyl acetate 20 ~ 35 DEG C of lixiviates, reclaim organic solvent, to obtain final product.
7. the mosquito-expelling and antipruritic Chinese medicine composition as described in Claims 1 to 4, is characterized in that: described Herba Origani oil is obtained by following method: by Adeps Bovis seu Bubali CO
2supercritical extraction and get final product, or by Adeps Bovis seu Bubali ethyl acetate, petroleum ether or ethanol 20 ~ 35 DEG C of lixiviates, reclaim ethyl acetate, petroleum ether or ethanol, to obtain final product.
8. the mosquito-expelling and antipruritic Chinese medicine composition as described in any one of claim 1 ~ 7 is preparing the application in mosquito-expelling and antipruritic medicine.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105816611A (en) * | 2016-04-15 | 2016-08-03 | 福建万亿店中店电子商务有限责任公司 | External use essential oil containing bamboo rat oil |
| CN106214745A (en) * | 2016-08-31 | 2016-12-14 | 防城港市防城区那梭香料厂 | A kind of compound recipe Oleum Cinnamomi nano-emulsion and preparation method thereof |
| CN110179693A (en) * | 2019-06-14 | 2019-08-30 | 江西中医药大学 | Mosquito-expelling and antipruritic compound essential oil and preparation method thereof, application and mosquito-expelling and antipruritic articles |
| CN110755346A (en) * | 2019-10-31 | 2020-02-07 | 洪雅县瓦屋山药业有限公司 | Mosquito-repelling and itching-relieving Coptis deltoidea toilet water and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816611A (en) * | 2016-04-15 | 2016-08-03 | 福建万亿店中店电子商务有限责任公司 | External use essential oil containing bamboo rat oil |
| CN106214745A (en) * | 2016-08-31 | 2016-12-14 | 防城港市防城区那梭香料厂 | A kind of compound recipe Oleum Cinnamomi nano-emulsion and preparation method thereof |
| CN110179693A (en) * | 2019-06-14 | 2019-08-30 | 江西中医药大学 | Mosquito-expelling and antipruritic compound essential oil and preparation method thereof, application and mosquito-expelling and antipruritic articles |
| CN110179693B (en) * | 2019-06-14 | 2022-09-16 | 江西中医药大学 | Mosquito-repelling itching-relieving compound essential oil, preparation method and application thereof, and mosquito-repelling itching-relieving article |
| CN110755346A (en) * | 2019-10-31 | 2020-02-07 | 洪雅县瓦屋山药业有限公司 | Mosquito-repelling and itching-relieving Coptis deltoidea toilet water and preparation method thereof |
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| CN104224925B (en) | 2016-08-24 |
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