CN104222528A - Compound amino acid feed additive and preparation method thereof - Google Patents
Compound amino acid feed additive and preparation method thereof Download PDFInfo
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- CN104222528A CN104222528A CN201410529137.6A CN201410529137A CN104222528A CN 104222528 A CN104222528 A CN 104222528A CN 201410529137 A CN201410529137 A CN 201410529137A CN 104222528 A CN104222528 A CN 104222528A
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Abstract
The invention discloses a compound amino acid feed additive and a preparation method thereof, wherein bean pulps are regarded as raw materials, after first and second fermentation, hydrolysis and dryness, the compound amino acid feed additive containing 50% amino acid is prepared. In the preparation method of the compound amino acid feed additive, the pot-adhering, bonding or caking problems cannot be occurred in the production process, thus the production efficiency is improved, the production process is simplified, the raw materials are cheap and easily obtained, and the cost is also obviously reduced, in comparison with the same-content compound amino acid powder, the price of the compound amino acid feed additive prepared by the method can be reduced by more than 75%, and the additive is directly blended into the feed to feed animals, as a result, the animals are increased by 5-10% in weight.
Description
[technical field]
The present invention relates to a kind of composite aminoacid feedstuff additive and preparation method thereof, be specifically related to a kind of method utilizing dregs of beans to prepare composite aminoacid feedstuff additive, belong to field of feed.
[background technology]
Protein feed resource deficiency is the bottleneck problem of puzzlement China animal husbandry healthy and stable development always, in the case, utilizes a series of agricultural byproducts Preparation of amino acid feed addictives with dregs of beans by representative be progressively familiar with by breeding production person and accept.Soyabean expeller (abbreviation dregs of beans) is the byproduct that soybean is left after extracting fat, generally uses as feed; Crude protein in dregs of beans is carried out fine finishining, makes it become compound amino acid, then add in feed, can greatly improve its added value.Except 43% crude protein, dregs of beans is also containing following component: moisture content≤13%, fat 0.2%, dietary fiber >=29.5%, carbohydrate about 7.7%, ash content about 4.7%, thiamine about 0.058%, niacin about 0.125%, and the trace element such as vitamin E, soybean ketone, urease, polysaccharide and potassium, sodium, calcium, magnesium, iron, manganese, zinc, copper, phosphorus.Dregs of beans is utilized to prepare the process of compound amino acid in prior art, the interphase interaction of above-mentioned each component, side reaction is many, have the phenomenon such as condensation, adhesion, affects degraded and the reaction speed of protein, and hydrolytic process may produce poisonous substance, the quantum of output of compound amino acid is low.
[summary of the invention]
For above-mentioned technical problem, the object of this invention is to provide a kind of composite aminoacid feedstuff additive and preparation method thereof, take dregs of beans as raw material, amino acid content is obtained close to 50% composite aminoacid feedstuff additive through first time fermentation, second time fermentation, hydrolysis and drying, this additive can directly admix feeding animals in feed, can make to be increased weight 5-10% by letting animals feed.
For achieving the above object, the invention provides following technical scheme, a kind of preparation method of composite aminoacid feedstuff additive, comprises the following steps:
(1) first time fermentation: the Soybean Meal after pulverizing is loaded in round, adds saccharomycete water and be fully uniformly mixed, add a cover the 24h that ferments at normal temperatures.
(2) second time fermentation: dregs of beans, after saccharomycetes to make fermentation, adds lactic acid bacteria water and is fully uniformly mixed, adds a cover and continues fermentation 24h at normal temperatures.
(3) be hydrolyzed: the watery hydrochloric acid of 5% of the dregs of beans after saccharomycete and lactobacillus-fermented carries out acid hydrolytic reaction, after fermentation, dregs of beans and watery hydrochloric acid are than being 1:1-1:1.4, hydrolysis temperature is 105-110 DEG C, constant temperature is hydrolyzed 6 hours, after being cooled to room temperature, utilize the mixture after NaOH neutralizing hydrolysis.
(4) dry: the mixture that step (3) obtains is drying to obtain composite aminoacid feedstuff additive.
In described step (1), the saccharomycete content of saccharomycete water is 0.1 hundred million/gram, and the ratio of dregs of beans and saccharomycete water is 1: 0.5-1:0.8; The content of lactic acid bacteria 5,000,000/gram of lactic acid bacteria water in described step (2), the ratio of dregs of beans and lactic acid bacteria water is 1: 0.3-1:0.6.
Preferably, in described step (1), the ratio of dregs of beans and saccharomycete water is 1: 0.6; In described step (2), the ratio of dregs of beans and lactic acid bacteria water is 1: 0.4.
Hydrolysis device preferably enamel reaction still in described step (3), after fermentation, the ratio of dregs of beans and watery hydrochloric acid is preferably 1:1.1, and hydrolysis temperature is 106 DEG C, adopts outer drainpipe to lower the temperature.
Dry run in described step (4) adopts reciprocating drying machine dry, and thermal source is 220V alternating current.
Advantage of the present invention and beneficial effect are:
(1) the present invention utilizes saccharomycete to carry out first time fermentation, saccharomycete is liked being attached to dietary fiber and is bred, in course of fermentation, solve the problem that dietary fiber is bonded in hydrolytic process, the saccharomycetic metabolin of sweat can produce amino acid and protein, can as the protein sources of hydrolytic process.
(2) lactic acid bacteria that in the present invention, second time fermentation adopts can utilize soybean polyoses, soybean ketone, thiamine, fat, niconacid, vitamin E, phosphorus and urease to carry out self-replication expansion as foodstuff, solves the problem reacted to each other between above-mentioned substance in hydrolytic process.Also produce metabolin amino acid and protein, as protein sources simultaneously.
(3) the present invention adopts twice fermentation process, removes multiple ANFs in dregs of beans, produces sour odour material simultaneously, have obvious phagostimulating effect for young animal.And zymotechnique adopts liquid fermentation, makes nutrient and microorganism be in water-soluble state, makes microorganism fully activate, ensure that amino acid content and the stability of product.
(4) preparation method of composite aminoacid feedstuff additive of the present invention, its production process can not produce the problems such as sticky pot, bonding or caking, productive rate can be improved, and production process is simple, raw material is cheaply easy to get, can significantly reduce costs, compare with the compound amino-acid powder of equal volume, the price of the composite aminoacid feedstuff additive obtained according to the method for the invention can reduce by more than 75%, additive is directly admixed feeding animals in feed, can make to be increased weight 5-10% by letting animals feed.
[detailed description of the invention]
Below in conjunction with specific embodiment, the invention will be further described, but scope that the present invention comprises is not limited thereto.
Embodiment 1
A kind of composite aminoacid feedstuff additive, its preparation method comprises the following steps:
First time fermentation: take the dregs of beans 500kg after pulverizing, load in round, the amount being 1: 0.5 according to the ratio of raw material and saccharomycete water adds lactic acid bacteria water (bacterium number content is 0.1 hundred million/gram), is fully uniformly mixed, ferment 24 hours, fermentation temperature is normal temperature.Saccharomycete is liked being attached to dietary fiber and is bred, and in course of fermentation, solves the problem that dietary fiber is bonded in hydrolytic process, and the saccharomycetic metabolin of sweat can produce amino acid and protein, can as the protein sources of hydrolytic process.
Second time fermentation: dregs of beans is after saccharomycetes to make fermentation, and add lactic acid bacteria water (bacterium number content is 5,000,000/gram), raw material and saccharomycete water ratio are 1: 0.6, are fully uniformly mixed, continue fermentation 24 hours, fermentation temperature is normal temperature.Lactic acid bacteria can utilize soybean polyoses, and soybean ketone, thiamine, fat, niconacid, vitamin E and phosphorus carry out self-replication expansion as foodstuff, solves the problem reacted to each other between above-mentioned substance in hydrolytic process, produces amino acid and protein simultaneously.
Hydrolysis: the dregs of beans after saccharomycete and lactobacillus-fermented carries out acid hydrolytic reaction in enamel reaction still (model: F0.25-2000DNPC), acid used is the watery hydrochloric acid of 5%, after fermentation, dregs of beans and watery hydrochloric acid are than being 1:1, hydrolysis temperature is 110 DEG C, constant temperature, after 6 hours, adopts outer drainpipe to be cooled to room temperature.Dregs of beans after acidic hydrolysis has compound amino acid blank, now get the compound amino acid that 100 grams of acidolysis obtain, acidometer is utilized to detect its pH value, convert through chemistry and draw the use amount of NaOH, adopt the dregs of beans after NaOH neutralizing hydrolysis, this process can not destroy the trace elements such as potassium, calcium, magnesium, iron, manganese, zinc, copper.
Dry: with the mixture after reciprocating drying machine drying hydrolysis, thermal source is 220V alternating current.Drying can obtain composite aminoacid feedstuff additive, and with reference to amino acid whose assay method in GB/T18246-2000 feed, recording amino acid whose content in this additive is 49.2%.
Embodiment 2
A kind of composite aminoacid feedstuff additive, its preparation method comprises the following steps:
First time fermentation: take the dregs of beans 500kg after pulverizing, load in round, the amount being 1: 0.8 according to the ratio of raw material and saccharomycete water adds lactic acid bacteria water (bacterium number content is 0.1 hundred million/gram), is fully uniformly mixed, ferment 24 hours, fermentation temperature is normal temperature.
Second time fermentation: dregs of beans is after saccharomycetes to make fermentation, and add lactic acid bacteria water (bacterium number content is 5,000,000/gram), raw material and saccharomycete water ratio are 1: 0.3, are fully uniformly mixed, continue fermentation 24 hours, fermentation temperature is normal temperature.
Hydrolysis: the dregs of beans after saccharomycete and lactobacillus-fermented carries out acid hydrolytic reaction in enamel reaction still (model: F0.25-2000DNPC), acid used is the watery hydrochloric acid of 5%, after fermentation, dregs of beans and watery hydrochloric acid are than being 1:1.4, hydrolysis temperature is 105 DEG C, constant temperature, after 6 hours, adopts outer drainpipe to be cooled to room temperature.Dregs of beans after acidic hydrolysis has compound amino acid blank, now gets the compound amino acid that 100g acidolysis obtains, and utilizes acidometer to detect its pH value, adopts the dregs of beans after NaOH neutralizing hydrolysis, and converting through chemistry draws the use amount of NaOH.
Dry: with mixture after reciprocating drying machine drying hydrolysis, thermal source is 220V alternating current.Drying can obtain composite aminoacid feedstuff additive, and with reference to amino acid whose assay method in GB/T18246-2000 feed, recording amino acid whose content in this additive is 48.9%.
Embodiment 3
A kind of composite aminoacid feedstuff additive, its preparation method comprises the following steps:
First time fermentation: take the dregs of beans 500kg after pulverizing, load in round, the amount being 1: 0.6 according to the ratio of raw material and saccharomycete water adds lactic acid bacteria water (bacterium number content is 0.1 hundred million/gram), is fully uniformly mixed, ferment 24 hours, fermentation temperature is normal temperature.
Second time fermentation: dregs of beans is after saccharomycetes to make fermentation, and add lactic acid bacteria water (bacterium number content is 5,000,000/gram), raw material and saccharomycete water ratio are 1: 0.4, are fully uniformly mixed, continue fermentation 24 hours, fermentation temperature is normal temperature.
Hydrolysis: the dregs of beans after saccharomycete and lactobacillus-fermented carries out acid hydrolytic reaction in enamel reaction still (model: F0.25-2000DNPC), acid used is the watery hydrochloric acid of 5%, after fermentation, dregs of beans and watery hydrochloric acid are than being 1:1.1, hydrolysis temperature is 106 DEG C, constant temperature, after 6 hours, adopts outer drainpipe to be cooled to room temperature.Dregs of beans after acidic hydrolysis has compound amino acid blank, now gets the compound amino acid that 100g acidolysis obtains, and utilizes acidometer to detect its pH value, adopts the dregs of beans after NaOH neutralizing hydrolysis, and converting through chemistry draws the use amount of NaOH.
Dry: with the mixture after reciprocating drying machine drying hydrolysis, thermal source is 220V alternating current.Drying can obtain composite aminoacid feedstuff additive, and with reference to amino acid whose assay method in GB/T18246-2000 feed, recording amino acid whose content in this additive is 49.7%.
Embodiment 4
Select individual about initial weight 8.2kg (28 age in days), athletic delactational piglets 60, stochastic averagina is divided into 2 groups: test group and control group, often organize 3 repetitions, each repetition 10 pigs, be mixed with 3% composite aminoacid feedstuff additive obtained according to this method in the feed that wherein test group adopts, raise 90 ages in days.Test is carried out your Kang Nongye Co., Ltd comprehensive testing ground green in Raoping County of Guangdong Province.Test adopts free choice feeding, drinking-water, and test pig house temperature, humidity, illumination are determined by test site routine.On-test and at the end of weigh the initial weight of each repeating groups piglet respectively and end is heavy, calculate its weightening finish (weightening finish=end weight-initial weight).Fasting 8 hours before at every turn weighing.
Experimental result is in table 1:
Table 1: the control-animal result of the test of embodiment 4
| Mensuration project | Control group | Test group |
| Group head number (head) | 30 | 30 |
| Initial weight (kg/ head) | 8.3 | 8.2 |
| End heavy (kg/ head) | 70.8 | 75.3 |
Result of the test shows, the average weight gain amount of test group piglet is higher than control group by 6.4%, illustrates that composite aminoacid feedstuff additive the present invention obtained directly is admixed in feed, can significantly improve the body weight growth rate of piglet.
Embodiment 5
Select individual about initial weight 31kg (60 age in days), athletic piggy 60, stochastic averagina is divided into 2 groups: test group and control group, often organize 3 repetitions, each repetition 10 pigs, be mixed with 3% composite aminoacid feedstuff additive obtained according to this method in the feed that wherein test group adopts, raise 120 ages in days.Test is carried out your Kang Nongye Co., Ltd comprehensive testing ground green in Raoping County of Guangdong Province.Test adopts free choice feeding, drinking-water, and test pig house temperature, humidity, illumination are determined by test site routine.On-test and at the end of weigh the initial weight of each repeating groups piggy respectively and end is heavy, calculate its weightening finish (weightening finish=end weight-initial weight).Fasting 8 hours before at every turn weighing.
Experimental result is in table 2:
Table 2: the control-animal result of the test of embodiment 5
| Mensuration project | Control group | Test group |
| Group head number (head) | 30 | 30 |
| First body counterpoise (kg/ head) | 31.2 | 31.1 |
| Opisthosoma counterpoise (kg/ head) | 103.8 | 112.1 |
Result of the test shows, compares with control group, and the average weight gain of test group piggy improves 8.0%, consistent with the check experiment result in embodiment 4.
Claims (8)
1. a preparation method for composite aminoacid feedstuff additive, is characterized in that: comprise the following steps:
(1) first time fermentation: the dregs of beans after pulverizing is loaded in round, adds saccharomycete water and be fully uniformly mixed, add a cover the 24h that ferments at normal temperatures;
(2) second time fermentation: dregs of beans, after saccharomycetes to make fermentation, adds lactic acid bacteria water and is fully uniformly mixed, adds a cover and continues fermentation 24h at normal temperatures;
(3) be hydrolyzed: the watery hydrochloric acid of 5% of the dregs of beans after saccharomycete and lactobacillus-fermented carries out acid hydrolytic reaction, after fermentation, dregs of beans and watery hydrochloric acid are than being 1:1-1:1.4, hydrolysis temperature is 105-110 DEG C, constant temperature is hydrolyzed 6 hours, after being cooled to room temperature, utilize the mixture after NaOH neutralizing hydrolysis;
(4) dry: the mixture that step (3) obtains is drying to obtain composite aminoacid feedstuff additive.
2. the preparation method of composite aminoacid feedstuff additive according to claim 1, is characterized in that: in described step (1), the saccharomycete content of saccharomycete water is 0.1 hundred million/gram, and the ratio of dregs of beans and saccharomycete water is 1: 0.5-1:0.8; The content of lactic acid bacteria 5,000,000/gram of lactic acid bacteria water in described step (2), the ratio of dregs of beans and lactic acid bacteria water is 1: 0.3-1:0.6.
3. the preparation method of composite aminoacid feedstuff additive according to claim 2, is characterized in that: in described step (1), the ratio of dregs of beans and saccharomycete water is 1: 0.6; In described step (2), the ratio of dregs of beans and lactic acid bacteria water is 1: 0.4.
4. the preparation method of composite aminoacid feedstuff additive according to claim 1, it is characterized in that: the hydrolysis device in described step (3) is enamel reaction still, after fermentation, the ratio of dregs of beans and watery hydrochloric acid is 1:1.1, and hydrolysis temperature is 106 DEG C, adopts outer drainpipe to cool.
5. the preparation method of composite aminoacid feedstuff additive according to claim 1, is characterized in that: the dry run of described step (4) adopts reciprocating drying machine dry, and thermal source is 220V alternating current.
6. a composite aminoacid feedstuff additive, is characterized in that: described composite aminoacid feedstuff additive is that the method according to any one of claim 1-5 is obtained.
7. a feed, is characterized in that: containing composite aminoacid feedstuff additive according to claim 6 in described feed.
8. feed according to claim 7, is characterized in that: the content of described composite aminoacid feedstuff additive is 3%.
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| CN114504063A (en) * | 2021-12-17 | 2022-05-17 | 潮州市东饶科技有限公司 | Compound amino acid feed protected by agar coating and preparation method thereof |
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Cited By (2)
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