CN104222075B - A kind of formula of trichoderma harzianum microbial inoculum carrier and preparation technology thereof - Google Patents
A kind of formula of trichoderma harzianum microbial inoculum carrier and preparation technology thereof Download PDFInfo
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- CN104222075B CN104222075B CN201410433891.XA CN201410433891A CN104222075B CN 104222075 B CN104222075 B CN 104222075B CN 201410433891 A CN201410433891 A CN 201410433891A CN 104222075 B CN104222075 B CN 104222075B
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Abstract
The present invention relates to biological technical field, specifically a kind of temperature sensitive Trichoderma microbial inoculum carrier formula and preparation technology thereof.A temperature sensitive Trichoderma microbial inoculum carrier formula, is characterized in that: described microbial inoculum carrier formula is formed by following raw-material weight percentage proportioning: organic weathered coal 35 ~ 45%; Humic acid 25 ~ 35%; Trichoderma fermenation raw liquid 15 ~ 25%; Diatomite 5 ~ 15%.Compared with the existing technology, biological beneficial microbial inoculum can be used for the multiple facilities such as outdoor cultivation and booth work.Rehabilitating soil and prevent and treat soil-borne disease.As various vegetables, cereal crops, fruit, medicinal material, flowers, nursery stock etc., can effectively prevent and treat stem rot, fusarium wilt, damping off, stalk break, damping off, root rot.Trichoderma and the microbial interaction such as plant, pathogen are the mutual work between Trichoderma, plant, pathogen three kinds of biologies in essence.
Description
Technical field
The present invention relates to biological technical field, specifically a kind of formula of trichoderma harzianum microbial inoculum carrier and preparation technology thereof.
Background technology
Soil-borne disease is prevented and treated, microbes soil-borne pathogen in main attack soil-borne disease with biological beneficial bacterium research and development.Soil-borne pathogen is generally by soil infection, and invaded plants root, stem, blade aperture or wound, cause crop root and even complete stool disease, cause serious financial consequences.Conventional chemical control at present and screening crop disease-resistant kind, only effective to minority soil-borne disease, very micro-to most of soil-borne disease preventive effect, and use chemical pesticide also can cause residue of pesticide, contaminated environment.Chemical pesticide Long-Time Service, makes pathogen pesticide resistance strengthen simultaneously, causes preventive effect to decline even failed.
In biological beneficial microbial inoculum, trichoderma has wide adaptability, broad-spectrum sterilization, noresidue, and the effectiveness phase is long, and action site is many, is not afraid of high humidity, and the feature such as do not develop immunity to drugs, harmless to people, animal, Environmental security, is a kind of green microbial inoculum.Use Trichoderma microbial inoculum effectively to break away from worry that soil-borne disease brings and loss.
Summary of the invention
The present invention is for overcoming the deficiencies in the prior art, and trichoderma harzianum is the beneficial microbe separated from agricultural land soil, belongs to fungal microbe, cooks microbial inoculum and suppresses harmful bacteria, makees fertilizer and encourages volume increase.
For achieving the above object, design a kind of preparation technology of trichoderma harzianum microbial inoculum carrier, it is characterized in that: described microbial inoculum carrier formula is formed by following raw-material weight percentage proportioning: organic weathered coal 35 ~ 45%; Humic acid 25 ~ 35%; Trichoderma fermenation raw liquid 15 ~ 25%; Diatomite 5 ~ 15%.
Described Trichoderma is
trichodermaHarzianumsH2303CGMCCNO4963 bacterial classification.
Following processing step is adopted to be prepared from: organic weathered coal and humic acid are poured in agitator and be uniformly mixed by (1), and agitator rotating speed is at 80 revs/min; (2) organic weathered coal after stirring and humic acid mixture are poured in warm air drier carry out drying and processing, water content is remained on and is less than 13%; (3) Trichoderma fermenation raw liquid is sprayed into droplet shape and enters dry rear organic weathered coal and humic acid mixture, every 5kg Trichoderma fermenation raw liquid is sprayed in the organic weathered coal of 50kg and humic acid compound particles; (4) organic weathered coal after spraying and humic acid mixture are poured in turbine tank carry out drying, add diatomite simultaneously and spread upon particle skin; Temperature controls at 30 ~ 50 DEG C, and dry 1 hour, water content remained on 10 ~ 12% and makes microbial inoculum carrier; (5) microbial inoculum carrier after drying is entered bag and weigh sealing, bacteria containing amount 200,000,000/gram.
Described weathered coal easily twists into pieces, and in pitchy after broken, final products outward appearance is the black core of white shell.
Described microbial inoculum carrier adds the anhydrous slaked lime 1 ~ 2% of solubility moderate-element, anhydrous magnesium sulfate 3 ~ 5% by demand, micronutrient element anhydrous zinc sulfate 1 ~ 2%, borax 0.1 ~ 0.5% or other microbial inoculum additives etc.
Compared with the existing technology, biological beneficial microbial inoculum can be used for the multiple facilities such as outdoor cultivation and booth work in the present invention.Rehabilitating soil and prevent and treat soil-borne disease.As various vegetables, cereal crops, fruit, medicinal material, flowers, nursery stock etc., can effectively prevent and treat stem rot, fusarium wilt, damping off, stalk break, damping off, root rot.Trichoderma and the microbial interaction such as plant, pathogen are the mutual work between Trichoderma, plant, pathogen three kinds of biologies in essence.
Organic weathered coal humic acid: a kind of natural organic high-molecular compound of humic acid, the usual and ion forming complex at occurring in nature.Humic acid is not single acid but a complex mixture containing the functional group such as carboxyl, phenolic hydroxyl group, shows the character of binary acid or ternary acid.Resource of humic acid is some low-calorie coals, such as: peat, brown coal and weathered coal, humic acid content wherein reaches 10 ~ 80%.
Diatomite is white, canescence or terra brown etc., is fine and smooth, loose, the potent physical property of light weight, porous, water imbibition and permeability.Density is 0.3 ~ 0.5g/cm
3, Mo Shi hardness is 1 ~ 1.5, fusing point 1650-1750 DEG C, and pH value is neutral, nontoxic, and voidage reaches 80-90%, and can absorb itself weight 1.5-4 water doubly, be the non-conductor of thermoelectricity sound, stable chemical nature, is insoluble to strong acid, can be dissolved in highly basic.Diatomite fineness is 120 order ~ 1200 orders, can be made for the additive of all feeds.
Embodiment
Organic weathered coal 40%, humic acid 30%, Trichoderma fermenation raw liquid 20%, diatomite 10%.Organic weathered coal and humic acid particle predrying, water content control is being less than 13%; Enter in agitator to carry out stirring to roll, rotating speed controls at 80 revs/min; Trichoderma fermenation raw liquid is sprayed into droplet shape and enters dried particles, and every 5kg Trichoderma fermenation raw liquid is sprayed in the organic weathered coal of 50kg and humic acid particle; Organic weathered coal and humic acid particle are entered turbine tank dry, synchronously add 5 ~ 10% diatomite and spread upon particle skin, within about 1 hour, Trichoderma fermenation raw liquid is mixed thoroughly immediately dry, temperature controls at 30 ~ 50 DEG C, exit is reached in turbine tank, enter bag and weigh sealing, bacteria containing amount 200,000,000/gram, water content 12 ~ 13% makes temperature sensitive Trichoderma microbial inoculum carrier, new product quality index is up to standard.
Temperature sensitive Trichoderma microbial inoculum carrier adds the anhydrous slaked lime 1 ~ 2% of solubility moderate-element, anhydrous magnesium sulfate 3 ~ 5% by demand, micro-anhydrous zinc sulfate 1 ~ 2%, borax 0.1 ~ 0.5% or other microbial inoculum additives etc., suppresses harmful bacteria with beneficial bacterium, reduces disease.
Embodiment one
Trichoderma community is on the impact of tomato yield, and community three processes, and processes 1,2,3 three repetition, totally 9 process comparative trials, and each process community is general executes 100kg farmyard manure, and process 1 is 400 times of water liquid, and process 2 is 600 times of water liquid, and process 3 is clear water contrast.Processing 2 output compares according to increasing 5.1%, and output process 1 compares according to increasing 12%, increase yield significantly.
Trichoderma is on the impact of tomato per mu yield, and test is established process 1,2,3 three to process 1,2,3 three and repeated totally 9 process comparative trials, and each test is general executes 2000kg farmyard manure, process 3 is check plot, processing 2 output compares according to increasing 6.2%, and process 1 compares according to increasing 12%, increase yield significantly.Implemented by Pu Nan vegetable gardening field, Shanghai Pudong New Area, on June 5th, 2007 receives test report.
Embodiment two
Trichoderma community is on the impact of cucumber yield, process 1,2,3 three process, three repetitions, totally 9 process comparative trials, each process community 100kg farmyard manure process 3 is check plot, processing 2 output compares according to increasing 51.6%, process 1 output to compare according to increasing 51%, process 2 output and compare according to increasing 51.6%, increase yield significantly.Implemented by upper sea otter virtue vegetable gardening field, on February 2nd, 2007 receives test report.
Embodiment three
Trichoderma establishes process 1,2,3 to watermelon blight control efficiency, and process 3 is check plot, process 1,2 first time medication 40 days preventive effects 85.7%, 85.7%; Second time medication 50 days preventive effects 85%, 85%; Third time medication 60 days preventive effects 73%, 80%; 4th medication, 80 days preventive effects 68%, 56%, preventive effect is remarkable.
Trichoderma is on the impact of watermelon pol.If process 1,2,3, process 1 is 400 times of water liquid, and process 2 is 600 times of water liquid, and process 3 is clear water contrast, and process 1,2 melon heart pols are respectively than contrast increase by 11%, 11.4%; Melon limit pol process 1,2 is respectively than contrast increase by 31%, 15%; Implemented by upper sea otter virtue vegetable gardening field, on February 28th, 2007 receives test report.
Claims (2)
1. a preparation technology for trichoderma harzianum microbial inoculum carrier, is characterized in that: the formula of described microbial inoculum carrier is formed with percentage by weight proportioning by following raw material: organic weathered coal 35 ~ 45%; Humic acid 25 ~ 35%; Trichoderma fermenation raw liquid 15 ~ 25%; Diatomite 5 ~ 15%; Following processing step is adopted to be prepared from by above-mentioned raw material: organic weathered coal and humic acid are poured in agitator and be uniformly mixed by (1), and agitator rotating speed is at 80 revs/min; (2) organic weathered coal after stirring and humic acid mixture are poured in warm air drier carry out drying and processing, water content is remained on and is less than 13%; (3) Trichoderma fermenation raw liquid is sprayed into droplet shape and enters dry rear organic weathered coal and humic acid mixture, every 5kg Trichoderma fermenation raw liquid is sprayed in the organic weathered coal of 50kg and humic acid compound particles; (4) organic weathered coal after spraying and humic acid mixture are poured in turbine tank carry out drying, add diatomite simultaneously and spread upon particle skin; Temperature controls at 30 ~ 50 DEG C, and dry 1 hour, water content remained on 10 ~ 12% and makes microbial inoculum carrier; (5) microbial inoculum carrier after drying is entered bag and weigh sealing, bacteria containing amount 200,000,000/gram; Described Trichoderma is
trichodermaHarzianumsH2303CGMCCNO4963 bacterial classification.
2. the preparation technology of a kind of trichoderma harzianum microbial inoculum carrier according to claim 1, it is characterized in that: described microbial inoculum carrier adds the anhydrous slaked lime 1 ~ 2% of solubility moderate-element, anhydrous magnesium sulfate 3 ~ 5% by demand, micronutrient element anhydrous zinc sulfate 1 ~ 2%, borax 0.1 ~ 0.5% or other microbial inoculum additives.
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| CN105145641B (en) * | 2015-10-26 | 2018-03-09 | 深圳市前海馥稷生物科技发展有限公司 | A kind of granule containing trichoderma harzianum and chitosan oligosaccharide |
| CN110384015A (en) * | 2019-06-26 | 2019-10-29 | 山东慧泰华生物技术有限公司 | A kind of systematic treating method of Citrus Huanglongbing pathogen |
| CN112159274A (en) * | 2020-09-28 | 2021-01-01 | 山东土木启生物科技有限公司 | Microbial granular inoculant with biocontrol nutrition function |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072563A (en) * | 1991-11-27 | 1993-06-02 | 义马市科学技术委员会 | Germicide for chloro-mould and preparation thereof |
| WO1994005150A1 (en) * | 1992-09-10 | 1994-03-17 | Commonwealth Scientific And Industrial Research Organisation | Method for the storage of entomopathogenic nematodes |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072563A (en) * | 1991-11-27 | 1993-06-02 | 义马市科学技术委员会 | Germicide for chloro-mould and preparation thereof |
| WO1994005150A1 (en) * | 1992-09-10 | 1994-03-17 | Commonwealth Scientific And Industrial Research Organisation | Method for the storage of entomopathogenic nematodes |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
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