CN104215474A - Two-pipe saliva sampling device - Google Patents

Abstract

The invention discloses a two-pipe saliva sampling device. When the sampling device is used for sampling, inner threads (6) on an outer ring (1) are matched with outer threads (8) on an opening end of a centrifugal pipe (5), an inner plug (3) is positioned in the outer ring (1), the lower part of a silicone pipe (4) is connected with the inner plug (3) into a whole, the upper part of the silicone pipe (4) penetrates out of the outer ring (1), and filling fibers (9) are arranged in the silicone pipe (4); when the sampling device is used for storage, the inner threads (6) on the outer ring (1) are matched with outer threads (8) on the opening end of the centrifugal pipe (5), a washer (2) is positioned on the topmost end in the outer ring, the inner plug (3) is arranged below the washer, the silicone pipe (4) is connected with the inner plug into a whole, the lower part of the silicone pipe (4) penetrates out of the outer ring to be arranged in the centrifugal pipe (5), and the filling fibers (9) are arranged in the silicone pipe. By adopting the two-pipe saliva sampling device, the saliva sampling process is simplified, the discomfort in the sampling process is eliminated, the saliva sampling amount is increased, the sampling process is safe and sanitary, and the two-pipe saliva sampling device is particularly applicable to babies or tested crowds having diseases and being difficult to sample.

Description

Double tube saliva sampler

technical field

The invention relates to a double-pipe saliva sampler, which belongs to the technical field of biochemical analysis and detection equipment.

Background technique

In recent years, saliva, as a non-invasive testing sample, has received attention in many fields such as disease diagnosis, psychological monitoring, and sports medicine. The components in saliva often have a good correspondence with blood. Saliva samples have the advantages of non-invasiveness and easy sampling, and are easily accepted by the subjects. However, the concentration of target components in saliva is often significantly lower than that in blood, so saliva collection must be sufficient to meet the needs of instrument detection (usually on the order of milliliters). Most of the current methods of saliva collection still allow the subjects to spit out a sufficient amount of saliva directly in an appropriate container or use a sampling stick or a sampling strip made of filter paper that looks like a cotton swab, but these methods may be harmful to the subjects, especially children. Patients with a disease that is difficult to cooperate with have certain difficulties. For example, the method of directly spitting out is difficult for infants and young children, and the sampling stick or sampling strip makes the subjects feel uncomfortable and uncooperative, and there is even a risk of swallowing, and it is difficult to collect a sufficient amount of samples.

At present, in terms of patents for saliva sample collection devices, there are authorized invention patents: CN1703615A, CN102032998A, CN203490113U; utility model inventions: CN200948144Y, CN2776296Y, CN201188066Y, CN201852719U, CN2912528Y. Among them, CN201852719U and CN102032998A are mainly designed for the analysis of genes in saliva, and the sampling volume is relatively small; CN1703615A better solves the problems of sampling volume, hygiene and safety, etc., but the disadvantage is that the device is too large, which is not convenient for sampling large sample populations; CN200948144Y is mainly used for the diagnosis of oral diseases, so the sampling volume is not enough to detect some components in saliva. Existing patents can solve certain saliva collection problems, but there is no special design for the saliva collection process of infant subjects, which fails to alleviate the discomfort of children during the sampling process, and sampling will occur in actual sampling and analysis The problem of insufficient quantity and low sample quality, and the design of the existing patent does not take into account the pretreatment of saliva samples, so it is not convenient to use.

In response to these problems, the present invention fully considers the characteristics of infants and young children, and designs a double-tube sampler, which uses safe and hygienic materials and sampling methods that are acceptable to children and parents. The normal stimulation of salivary glands by high-grade citric acid enables children to secrete more saliva relatively easily. Therefore, the whole sampling process is safe, hygienic and convenient. The collected saliva has a large volume and can be used for the determination of various components.

Contents of the invention

Technical problem: the object of the present invention is to provide a double-tube saliva sampler, which solves the discomfort, safety, and insufficient sampling volume of the current test population in the saliva sample sampling process.

Technical scheme: in order to solve these problems in the prior art, the technical scheme provided by the invention is:

The double-tube saliva sampler of the present invention includes an outer ring, a gasket, an inner plug, a silicone tube, a centrifuge tube, and a filling fiber; when sampling, the inner thread on the outer ring coincides with the outer thread on the open end of the centrifuge tube, and the inner plug is positioned at the outer In the ring, the lower part of the silicone tube is integrated with the inner plug, and the upper part passes through the outer ring, and there is a filling fiber in the silicone tube; when in storage, the inner thread on the outer ring coincides with the outer thread on the opening end of the centrifuge tube, and the gasket is located At the top of the outer ring, below the gasket is an inner plug, the silicone tube is integrated with the inner plug, the lower part passes through the outer ring and is located in the centrifuge tube, and the silicone tube is provided with filling fibers.

The upper plane of the inner plug is a mesh structure.

The silicone tube is a porous structure with sparse upper part and denser lower part.

The filling fiber is cotton fiber or nano fiber material with special adsorption function, and the filling fiber is pre-treated with 5% citric acid solution.

The silicone tube is made of food-grade silicone material, about 4.5 cm long, with an outer diameter and an inner diameter of 1 cm and 0.9 cm, respectively, and an outer diameter of the upper edge of the silicone tube is 1.6 cm.

The inner plug is made of polypropylene plastic material with a hollow structure, one end is open and the other end is a mesh structure. The outer diameter of the plug body is 0.95 cm, the inner diameter is 0.85 cm, and the outer diameter of the upper part of the inner plug is 1.6 cm.

The method of use is as follows: when not sampling, the silicone tube is filled with a certain quality of filling material, and then the inner plug is inserted from the opening of the silicone tube, a gasket is placed on the edge of the inner plug, and finally the outer ring is fixed to the centrifuge tube through threads. When sampling, unscrew the outer ring, remove the gasket, take out the silicone tube connected to the inner plug and turn it over and place it on the centrifuge tube, then put the outer ring through the silicone tube and screw it on the centrifuge tube, so that the silicone tube and the The inner plug is fixed on the centrifuge tube and ready for use. When collecting saliva, put the silicone tube into the mouth of the subject, and the subject chews the silicone tube so that the saliva enters the fibrous material in the tube through the small hole on the tube wall and is absorbed. After sampling, the entire device was assembled back to its unsampled shape and placed in a centrifuge to centrifuge the adsorbed saliva into a centrifuge tube. The invention simplifies the saliva sampling process, eliminates the discomfort during the sampling process, increases the amount of saliva sampling, and is safe and hygienic during the sampling process, and is especially applicable to infants or young children or subjects with diseases who are difficult to cooperate with sampling. The collected saliva samples can be used for analysis and detection of various biochemical components.

Beneficial effect: compared with the prior art scheme, the advantages of the present invention are:

1. Children chew the silicone tube, and the saliva flows into the filling material in the silicone tube through the small holes on the tube to achieve the purpose of adsorption and enrichment. The material of the silicone tube is food-grade silicone, which is safe and reliable. The material of the silicone tube is soft, and the bottom end is processed into a hemispherical structure. When the subjects chew, they feel comfortable in the mouth and are easier to accept psychologically. The structure from dense to sparse allows saliva to flow smoothly to the bottom of the centrifuge tube after being separated from the filling fiber when centrifuging after sampling, so as to be retained in the centrifuge tube;

2. Using citric acid to treat the filled fiber adsorption material can make the subjects salivate more and faster, shorten the sampling time and increase the sampling volume;

3. The safety of the sampling device is guaranteed. According to the sampling device diagram in Figure 2, during sampling, the centrifuge tube can serve as a handle, and for subjects that are difficult to cooperate (such as infants), it can be controlled by the sampler. And the silicone tube and the centrifuge tube are screwed tightly in the middle, which is firm and not easy to fall off, preventing the risk of the sampling head being accidentally swallowed during the sampling process;

4. The double-tube structure design makes sample processing more convenient. After the child chews the inner silicone tube, put the inner silicone tube and the inner plug back into the outer centrifuge tube, pad the gasket, and then screw on the outer ring, and restore the entire device assembly to the form shown in Figure 1, which can be directly put into the centrifuge After centrifugation, the saliva sample can be stored in the refrigerator, which is convenient and quick.

Description of drawings

The present invention will be further described below in conjunction with accompanying drawing and embodiment:

Fig. 1 is the structural representation of double tube type saliva sampler,

Fig. 2 is the schematic diagram of the structure of the double-pipe saliva sampler when sampling,

Figure 3 is a schematic diagram of the inner silicone tube,

Figure 4 is a schematic diagram of the inner plug,

Figure 5 is a schematic diagram of the outer ring.

In the above figure, there are: outer ring 1, gasket 2, inner plug 3, silicone tube 4, centrifuge tube 5, internal thread 6, mesh structure 7, external thread 8, filling fiber 9, porous structure 10.

Detailed ways

The double-pipe saliva sampler of the present invention. (1) Use a food-grade silicone tube as the oral saliva enrichment device. The wall of the silicone tube is processed into a porous structure with a sparse top and a dense bottom, and the top is processed into a hemispherical structure similar to a pacifier to make the chewing process more comfortable; (2) use 5% citric acid solution handles the fiber-type adsorbent material as the filling material in the silica gel tube; (3) the dual-tube structure design of the inner silica gel tube and the outer centrifuge tube; specifically as follows:

The sampler includes an outer ring, gasket, inner plug, silicone tube, centrifuge tube, and filling fiber; when sampling, the inner thread on the outer ring coincides with the outer thread on the opening end of the centrifuge tube, the inner plug is located in the outer ring, and the silicone tube The lower part is integrated with the inner plug, the upper part passes through the outer ring, and there is a filling fiber in the silicone tube; during storage, the inner thread on the outer ring coincides with the outer thread on the opening end of the centrifuge tube, and the gasket is located at the top of the outer ring , under the gasket is an inner plug, the silicone tube is integrated with the inner plug, the lower part passes through the outer ring and is located in the centrifuge tube, and the silicone tube is provided with filling fibers.

The upper plane of the inner plug is a mesh structure.

The silicone tube is a porous structure with sparse upper part and denser lower part.

The filling fiber is cotton fiber or nano fiber material with special adsorption function, and the filling fiber is pre-treated with 5% citric acid solution.

1. The inner silicone tube is made of food-grade silicone material, about 4.5cm long, with an outer diameter and an inner diameter of about 1cm and 0.9cm respectively, and the outer diameter of the upper edge of the silicone tube is 1.6cm. Filled with fibrous absorbent material inside. The hemispherical structure at the bottom, and from the top to the bottom of the silicone tube, it is processed into a porous structure with a sparse upper part and a dense lower part (based on the direction placed in Figure 1);

2. The inner plug is made of polypropylene plastic and has a hollow structure. One end is open and the other end is a mesh structure. The outer diameter of the plug body is 0.95cm, the inner diameter is 0.85cm, and the outer diameter of the upper part of the inner plug is 1.6cm;

3. The filling fiber can be cotton fiber or nanofiber material with special adsorption function. The fiber can be pre-treated with 5% citric acid solution.

The above scheme will be further described below in conjunction with specific examples. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions adopted in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

The embodiment is shown in Fig. 1 and Fig. 2. The sampler comprises an outer ring 1, a gasket 2, an inner plug 3, a silicone tube 4, a centrifuge tube 5, and a filling fiber 9; during sampling, the internal thread 6 on the outer ring 1 coincides with the external thread 8 at the open end of the centrifuge tube 5, The inner plug 3 is located in the outer ring 1, the lower part of the silicone tube 4 is integrated with the inner plug 3, and the upper part passes through the outer ring 1, and a filling fiber 9 is arranged in the silicone tube 4; The thread 6 matches the external thread 8 at the opening end of the centrifuge tube 5, the gasket 2 is located at the top of the outer ring 1, the inner plug 3 is below the gasket 2, the silicone tube 4 is connected with the inner plug 3, and the lower part passes through the outer ring 1. The ring 1 is located in the centrifuge tube 5 , and the filling fiber 9 is arranged in the silicone tube 4 .

The upper plane of the inner plug 3 is a mesh structure 7 .

The silicone tube 4 is a porous structure 10 with a sparse upper part and a denser lower part.

The filling fibers 9 are cotton fibers or nanofiber materials with special adsorption function, and the filling fibers are pre-treated with 5% citric acid solution.

The outer centrifuge tube is made of polypropylene plastic (PP), the length is 7.5cm, the inner and outer diameters are about 1.4cm and 1.6cm respectively, and there are external threads carved from the top of the outer wall of the centrifuge tube down to 2cm;

As shown in Figure 3, the inner silicone tube is made of food-grade silicone tube, with a length of 4cm and an inner and outer diameter of about 0.9cm and 1cm respectively, and the lower end of the silicone tube is a hemispherical structure, which is processed from dense to sparse from the top Porous structure, the end of the silicone tube is processed into an extended edge shape, and the diameter of the edge is about 1.6cm. The inside of the silicone tube is filled with fiber-type adsorption materials, which are made of pure natural cotton or functional nanofibers made of food-grade polymer electrospun. The former has an adsorption effect on saliva and can be separated from the liquid phase after centrifugation, which is convenient for collecting saliva The latter can also have high-efficiency adsorption on certain targets in saliva, so that the test targets in saliva can be well enriched after sampling, and the saliva separated after centrifugation can be used for the detection of certain indicators, while nanofiber materials Some specific indicators can be determined by analyzing with appropriate methods after taking out;

As shown in Figure 4, the inner plug is made of polypropylene plastic and is about 2cm long. Its interior is a hollow structure, one end is open, and the other end is processed into a sieve structure with extended edges. The inner and outer diameters of the plug body are about 0.85cm and 0.95cm respectively, and the sieve holes are uniformly distributed small holes. About 1mm, the outer diameter at the edge is about 1.6cm;

As shown in Figure 5, the outer ring is made of polypropylene plastic. The inner and outer diameters of the outer ring are about 1.6cm and 1.8cm respectively, and the length is 2cm. The outer ring is a hollow cylinder structure. The diameter of the opening at one end is slightly smaller, about 1.2cm, and the opening diameter at the other end is the same as the inner diameter, which is 1.6cm. The ring is engraved with internal threads;

The gasket is a disc-like structure made of rubber, with a diameter of about 1.6cm and a thickness of 0.5-1mm.

The above invention is only to illustrate the technical conception and characteristics of the present invention, and its purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly, and not to limit the scope of protection of the present invention. All equivalent exchanges or modifications made according to the spirit of the present invention shall fall within the protection scope of the present invention.

Claims (6)

1. a double hose saliva sampler, it is characterized in that, this sampling thief comprises outer shroud (1), pad (2), interior plug (3), silicone tube (4), centrifuge tube (5), fiberfill fibers (9); During sampling, internal thread (6) on outer shroud (1) coincide with the external thread (8) of centrifuge tube (5) openend, interior plug (3) is positioned at outer shroud (1), bottom and the interior plug (3) of silicone tube (4) are connected as a single entity, top passes outer shroud (1), is provided with fiberfill fibers (9) in silicone tube (4); When preserving, internal thread (6) on outer shroud (1) coincide with the external thread (8) of centrifuge tube (5) openend, pad (2) is positioned at the top of outer shroud (1), interior plug (3) below pad (2), silicone tube (4) and interior plug (3) are connected as a single entity, bottom passes outer shroud (1) and is positioned at centrifuge tube (5), is provided with fiberfill fibers (9) in silicone tube (4).

2. a kind of double hose saliva sampler according to claim 1, is characterized in that, the upper plane of described interior plug (3) is sieve pore structure (7).

3. a kind of double hose saliva sampler according to claim 1, is characterized in that, described silicone tube (4) is for top is sparse, the porous structure of lower dense (10).

4. a kind of double hose saliva sampler according to claim 1, is characterized in that, described fiberfill fibers (9) is cotton fiber or the nano-fiber material having special adsorption function, and fiberfill fibers uses 5% citric acid solution process in advance.

5. a kind of double hose saliva sampler according to claim 1, is characterized in that, described silicone tube (4) uses food grade silicone material, and be about 4.5cm, external diameter and internal diameter are respectively 1cm and 0.9cm, and on silicone tube, the external diameter on edge is 1.6cm.

6. a kind of double hose saliva sampler according to claim 1, it is characterized in that, described interior plug (3) is acrylic plastering material, hollow structure, one end open, the other end is sieve pore structure, and the external diameter of plug body is 0.95cm, internal diameter is 0.85cm, and the upper external diameter along part of interior plug is 1.6cm.