CN105911198A - Pretreatment device for furfural substance detection and application thereof - Google Patents
Pretreatment device for furfural substance detection and application thereof Download PDFInfo
- Publication number
- CN105911198A CN105911198A CN201610398175.1A CN201610398175A CN105911198A CN 105911198 A CN105911198 A CN 105911198A CN 201610398175 A CN201610398175 A CN 201610398175A CN 105911198 A CN105911198 A CN 105911198A
- Authority
- CN
- China
- Prior art keywords
- absorbent material
- saliva
- sampling
- lid
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Abstract
The invention provides a pretreatment device for furfural substance detection and application thereof. The pretreatment device comprises a conical plastic tube (1), a sampling needle (2), a sampling syringe (3), a water-absorbing material (4), a water-absorbing material pipe (5) with a hole in the bottom, a conical plastic tube cover (6), a water-absorbing material pipe cover (7), a sampling needle push rod (8) and an extraction solution. The sampling needle is connected with the sampling syringe, and the sampling syringe is connected with the sampling needle push rod to form a sampler; the water-absorbing material is placed in the water-absorbing material pipe with the hole in the bottom, the water-absorbing material pipe cover is arranged on the upper portion of the water-absorbing material pipe, and accordingly a saliva temporary storage device is formed; the sampler and the saliva temporary storage device are arranged in the conical plastic tube, the sampling needle can reach the position below the liquid surface of the extraction solution, and the sampling needle push rod and the water-absorbing material cover are arranged on the conical plastic tube cover.
Description
Technical field
The invention belongs to technical field of analytical chemistry, be specifically related to a kind of be exclusively used in saliva the detection of bran aldehyde material before
Processing means and application.
Background technology
Furfural, also known as 2 furan carboxyaldehyde, is same substance with alditol.Its formal name used at school is furaldehyde, is on 2, furan
The derivant that hydrogen atom is replaced by aldehyde radical；Furfural is to be hydrolyzed generation pentose under the action of an acid by pentosan, then by dehydration of pentoses
Cyclisation forms；Furfural is the most important derivant of furan ring system, and chemical property is active, can be by reaction systems such as oxidation, condensations
Take numerous derivants, be widely used in the industry such as synthetic plastic, medicine, pesticide.5 methyl furfural is that a kind of food adds
Agent.5 hydroxymethyl furfural is dehydrated by glucose or fructose and generates, containing a furan nucleus in molecule, and an aldehyde radical and a hydroxyl first
Base, its chemical property is relatively more active, can be reacted by aoxidize, hydrogenate and condensation etc. and prepare multiple derivant, be important essence
Thin industrial chemicals.
Bran aldehyde material in saliva is studied to the property of health risk when being conducive to the explanation mankind to be exposed to this type of material
Matter and degree.Due to saliva sample not easily collecting and preservation, in saliva, bran aldehyde matter content is relatively low, and detection is to disturb relatively greatly,
Requirement to sample pre-treatments is higher, therefore, develop a kind of pretreating device being exclusively used in the detection of bran aldehyde material in saliva and
Using method is particularly important.
Summary of the invention
It is an object of the invention to provide pretreating device and the application of bran aldehyde material detection in a kind of saliva, have fast
Speed, accurate, simple operation and other advantages.
The technical solution adopted in the present invention is: this device by conical plastics pipe, sampling probe, sampling syringe, absorbent material,
The absorbent material pipe of bottom of which has holes, conical plastics lid, absorbent material lid, sampling probe push rod and extraction solution composition, sampling
Pin connects sampling syringe, and sampling syringe connects sampling probe push rod and constitutes sampler；Absorbent material is placed in the water-absorption material of bottom of which has holes
In material pipe, absorbent material pipe top is absorbent material lid, thus constitutes saliva buffer, and sampler and saliva buffer are placed in
In conical plastics pipe, the length of sampling probe reaches to extract below liquid level of solution, and sampling probe push rod and absorbent material lid are all at cone
Above shape plastic tubular cover.Described extraction solution is dichloromethane.
Described conical plastics pipe and plastic tubular cover constitute airtight centrifuge tube, for centrifugation and deposit sample, centrifugal
It is 0 ~ 5 mL that pipe is provided with scale, good airproof performance.
The described hole diameter bottom bottom of which has holes absorbent material pipe is 2 ~ 4mm, saliva outflow in centrifugation step,
The addition of deicer in extractant addition, drying steps in extraction step.
A diameter of 1 ~ 1.5cm of described sampling syringe (3).
The application of the pretreating device of bran aldehyde material detection in saliva:
Open absorbent material lid, take out absorbent material and put in smoker oral cavity, after absorbent material is completely moistening, take rapidly
Go out to put back to bottom of which has holes absorbent material pipe and be sealed against；After centrifugal 2 ~ 5 min, the saliva sample of clarification collects plastic tube
Bottom；Open absorbent material lid, take absorbent material away, add 1 ~ 3 mL dichloromethane extraction solution, with the saliva of bottom
Sample fully mixes, and stands 3 ~ 5 min and is layered as dichloromethane layer and water layer；Pull out sampling probe push rod, by under 1 ~ 2 mL
Layer dichloromethane extract takes to sampling syringe, takes off sampler, is transferred in chromatogram bottle by sample, enters GC/MS and analyzes, suitable
The bran aldehyde materials such as furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural are detected for simultaneously.
In the saliva of the present invention, pretreating device and the application of the detection of bran aldehyde material have the positive effect that:
(1) step is simple, the method only need to be centrifuged, extract after can sample introduction analysis, process step simple, this invention simultaneously provides
One-stop pretreating device, it is easy to promote；
(2) accuracy is high, and the method completes pre-treatment in airtight system, and step is simple, greatly reduces analysans
Loss in purifying transfer process, adds the accuracy of analysis process；
(3) cost is relatively low, plastic tube that this device relates to, sampling probe, sampling syringe, absorbent material, bottom of which has holes absorbent material
Pipe, plastic tubular cover, absorbent material lid and sampling probe push rod etc., the material being easy to get for laboratory is prepared from, and cost is relatively low；
(4) environmental friendliness, device good airproof performance, sample is not easy volatilization, and this invention, while improving precision of analysis, reduces
The pretreatment process pollution to environment.
The present invention is the detection of bran aldehyde material in saliva, has quick, accurate, simple operation and other advantages.
Accompanying drawing explanation
Fig. 1 be the present invention saliva in bran aldehyde material detection pretreating device structural representation.
In figure: 1-conical plastics pipe, 2-sampling probe, 3-sample syringe, 4-absorbent material, 5-bottom of which has holes absorbent material
Pipe, 6-conical plastics lid, 7-absorbent material lid, 8-sampling probe push rod, 9-dichloromethane layer, 10-saliva layer.
Detailed description of the invention
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and should not be regarded as limiting this
Bright scope.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition
Or carry out according to product description.Agents useful for same or instrument unreceipted production firm person, be and can be obtained by purchase
Conventional products.
The invention will be further described below in conjunction with the accompanying drawings.
Embodiment 1
As it is shown in figure 1, in the saliva of the present invention bran aldehyde material detection pretreating device, by conical plastics pipe 1, sampling probe 2,
Sampling syringe 3, absorbent material 4, the absorbent material pipe 5 of bottom of which has holes, conical plastics lid 6, absorbent material lid 7, sampling probe
Push rod 8 and extraction solution composition, sampling probe 2 connects sampling syringe 3, and sampling syringe 3 connects sampling probe push rod 8 and constitutes sampler,
Can change as required and unpick and wash；Absorbent material 4 is placed in the absorbent material pipe 5 of bottom of which has holes, and absorbent material pipe top is for inhaling
Water material lid 7, size matches with absorbent material lid 7, thus constitutes saliva buffer, can change as required and tear open
Wash.Sampler and saliva buffer are placed in conical plastics pipe 1, and the length of sampling probe reaches to extract below liquid level of solution, sampling
Pin push rod and absorbent material lid 7 are all on conical plastics lid 6.
Described extraction solution is dichloromethane.
Described conical plastics pipe 1 and conical plastics lid 6 constitute airtight centrifuge tube, for centrifugation and deposit sample
Product, it is 0 ~ 5 mL that centrifuge tube is provided with scale, good airproof performance.
The described hole diameter bottom bottom of which has holes absorbent material pipe 5 is 2 ~ 4mm, saliva stream in centrifugation step
Go out, in extraction step extractant add, the addition of deicer in drying steps.
A diameter of 1 ~ 1.5cm of described sampling syringe 3.
Application Example 1:
Open absorbent material lid 7, take out absorbent material 4 and put in smoker oral cavity, after absorbent material is completely moistening, rapidly
Taking-up is put back to bottom of which has holes absorbent material pipe 5 and is sealed against；After centrifugal 2 min, the saliva sample of clarification collects plastics
The bottom of pipe 1；Open absorbent material lid 7, take absorbent material 4 away, add 2 mL dichloromethane extraction solution, with the saliva of bottom
Liquid sample fully mixes, and stands 3 min and is layered as dichloromethane layer 9 and water layer 10；Pull out sampling probe push rod 8, by 1 mL
Lower floor's dichloromethane extract 9 takes to sampling syringe 3, takes off sampler, is transferred in chromatogram bottle by sample, enters GC/MS and divide
Analysis, can detect the bran aldehyde materials such as furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural simultaneously.
Application Example 2:
Open absorbent material lid 7, take out absorbent material 4 and put in smoker oral cavity, after absorbent material is completely moistening, rapidly
Taking-up is put back to bottom of which has holes absorbent material pipe 5 and is sealed against；After centrifugal 5 min, the saliva sample of clarification collects plastics
The bottom of pipe 1；Open absorbent material lid 7, take absorbent material 4 away, add 3 mL dichloromethane extraction solution, with the saliva of bottom
Liquid sample fully mixes, and stands 5 min and is layered as dichloromethane layer 9 and water layer 10；Pull out sampling probe push rod 8, by 2 mL
Lower floor's dichloromethane extract 9 takes to sampling syringe 3, takes off sampler, is transferred in chromatogram bottle by sample, enters GC/MS and divide
Analysis, can detect the bran aldehyde materials such as furfural in saliva, 5 methyl furfural and 5 hydroxymethyl furfural simultaneously.
Claims (5)
1. the pretreating device of bran aldehyde material detection in a saliva, it is characterised in that: this device is by conical plastics pipe, sampling
Pin, sampling syringe, absorbent material, the absorbent material pipe of bottom of which has holes, conical plastics lid, absorbent material lid, sampling probe push away
Bar and extraction solution composition, sampling probe connects sampling syringe, and sampling syringe connects sampling probe push rod and constitutes sampler；Absorbent material
Being placed in the absorbent material pipe of bottom of which has holes, absorbent material pipe top is absorbent material lid, thus constitutes saliva buffer, takes
Sample device and saliva buffer are placed in conical plastics pipe, and the length of sampling probe reaches to extract below liquid level of solution, sampling probe push rod
With absorbent material lid all on conical plastics lid.
The pretreating device of bran aldehyde material detection in saliva the most according to claim 1, it is characterised in that: described cone
Shape plastic tube and conical plastics lid constitute airtight centrifuge tube, and for centrifugation and deposit sample, centrifuge tube is provided with scale
It is 0 ~ 5 mL, good airproof performance.
The pretreating device of bran aldehyde material detection in saliva the most according to claim 1, it is characterised in that the described end
Hole diameter bottom portion's absorbent material with holes pipe is 2 ~ 4mm, extracts molten in centrifugation step in saliva outflow, extraction step
The addition of deicer in agent addition, drying steps.
The pretreating device of bran aldehyde material detection in saliva the most according to claim 1, it is characterised in that: described takes
A diameter of 1 ~ 1.5cm of sample syringe (3).
5. the application of the pretreating device of bran aldehyde material detection in the saliva described in a claim 1, it is characterised in that: beat
Open absorbent material lid, take out absorbent material and put in smoker oral cavity, after absorbent material is completely moistening, takes out rapidly and put back to
Bottom of which has holes absorbent material pipe is also sealed against；After centrifugal 2 ~ 5 min, the saliva sample of clarification is collected under plastic tube
Portion；Open absorbent material lid, take absorbent material away, add 1 ~ 3 mL dichloromethane extraction solution, with the saliva sample of bottom
Fully mixing, stands 3 ~ 5 min and is layered as dichloromethane layer and water layer；Pull out sampling probe push rod, by 1 ~ 2 mL lower floor two
Chloromethanes extract takes to sampling syringe, takes off sampler, is transferred in chromatogram bottle by sample, enters GC/MS and analyzes, it is adaptable to
The bran aldehyde material such as furfural, 5 methyl furfural and 5 hydroxymethyl furfural in detection saliva simultaneously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610398175.1A CN105911198B (en) | 2016-06-07 | 2016-06-07 | A kind of pretreating device of chaff aldehyde material detection and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610398175.1A CN105911198B (en) | 2016-06-07 | 2016-06-07 | A kind of pretreating device of chaff aldehyde material detection and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105911198A true CN105911198A (en) | 2016-08-31 |
| CN105911198B CN105911198B (en) | 2018-03-09 |
Family
ID=56749848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610398175.1A Active CN105911198B (en) | 2016-06-07 | 2016-06-07 | A kind of pretreating device of chaff aldehyde material detection and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105911198B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111024907A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in liquid dairy product based on storage temperature and humidity of liquid dairy product |
| CN111024906A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in solid dairy product based on storage temperature and humidity of solid dairy product |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1006359A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Device and method for separating components of a liquid sample |
| WO2001070403A1 (en) * | 2000-03-17 | 2001-09-27 | Greiner Bio-One Gmbh | Collection vessel for separating biological fluid constituents |
| CN1790013A (en) * | 2004-12-16 | 2006-06-21 | 雅安三九药业有限公司 | Method for simultaneous determination of protocatechuic acid content and 5-hydroxymethyl furfural content in pulse-activating injection |
| US7387899B1 (en) * | 1995-07-11 | 2008-06-17 | D Angelo Joseph P | Saliva sample collection system |
| CN101762647A (en) * | 2008-12-26 | 2010-06-30 | 青岛啤酒股份有限公司 | Method for measuring 2-furfural in beer by using high performance liquid chromatography |
| CN103575835A (en) * | 2013-11-05 | 2014-02-12 | 陕西省食品药品检验所 | Method for determining 2-furfural in dextran-40 sodium chloride injection |
| CN104142372A (en) * | 2014-07-17 | 2014-11-12 | 中国石油化工股份有限公司 | Method for detecting furfuryl alcohol content in furfural biological hydrogenation reaction liquid |
| CN104215474A (en) * | 2014-09-03 | 2014-12-17 | 东南大学 | Two-pipe saliva sampling device |
| CN204797898U (en) * | 2015-07-09 | 2015-11-25 | 云南中烟工业有限责任公司 | Extrusion formula saliva sample collection device |
| CN204839578U (en) * | 2015-07-09 | 2015-12-09 | 云南中烟工业有限责任公司 | Saliva sample collection device |
-
2016
- 2016-06-07 CN CN201610398175.1A patent/CN105911198B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7387899B1 (en) * | 1995-07-11 | 2008-06-17 | D Angelo Joseph P | Saliva sample collection system |
| EP1006359A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Device and method for separating components of a liquid sample |
| WO2001070403A1 (en) * | 2000-03-17 | 2001-09-27 | Greiner Bio-One Gmbh | Collection vessel for separating biological fluid constituents |
| CN1790013A (en) * | 2004-12-16 | 2006-06-21 | 雅安三九药业有限公司 | Method for simultaneous determination of protocatechuic acid content and 5-hydroxymethyl furfural content in pulse-activating injection |
| CN101762647A (en) * | 2008-12-26 | 2010-06-30 | 青岛啤酒股份有限公司 | Method for measuring 2-furfural in beer by using high performance liquid chromatography |
| CN103575835A (en) * | 2013-11-05 | 2014-02-12 | 陕西省食品药品检验所 | Method for determining 2-furfural in dextran-40 sodium chloride injection |
| CN104142372A (en) * | 2014-07-17 | 2014-11-12 | 中国石油化工股份有限公司 | Method for detecting furfuryl alcohol content in furfural biological hydrogenation reaction liquid |
| CN104215474A (en) * | 2014-09-03 | 2014-12-17 | 东南大学 | Two-pipe saliva sampling device |
| CN204797898U (en) * | 2015-07-09 | 2015-11-25 | 云南中烟工业有限责任公司 | Extrusion formula saliva sample collection device |
| CN204839578U (en) * | 2015-07-09 | 2015-12-09 | 云南中烟工业有限责任公司 | Saliva sample collection device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111024907A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in liquid dairy product based on storage temperature and humidity of liquid dairy product |
| CN111024906A (en) * | 2019-12-26 | 2020-04-17 | 光明乳业股份有限公司 | Method for rapidly evaluating content of furfural substances in solid dairy product based on storage temperature and humidity of solid dairy product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105911198B (en) | 2018-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kataoka | Current developments and future trends in solid-phase microextraction techniques for pharmaceutical and biomedical analyses | |
| CN104991017B (en) | Liquid chromatogram-tandem mass spectrometry method for determining isothiazolinone bactericides contained in water-based adhesive | |
| CN102419352B (en) | Determination method of amount of phthalic acid esters | |
| CN104502617A (en) | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue | |
| CN102175778A (en) | Method for synchronously measuring blood drug concentrations of multiple antidepressants | |
| CN103543224A (en) | Detection method for residues of abamectin and ivermectin | |
| CN106124639A (en) | The multicomponent content assaying method of Eucommia ulmoides | |
| CN104330489B (en) | A kind of detection method of dispelling wind detoxicating capsule | |
| CN105806840B (en) | The kit and detection method of paraquat in qualitative and half-quantitative detection serum or urine | |
| CN105911198A (en) | Pretreatment device for furfural substance detection and application thereof | |
| CN105548392A (en) | Method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography | |
| CN103995074B (en) | Method for detecting residual quantity of organochlorine pesticides in raw milk | |
| CN101690886A (en) | Molecular imprinting monolithic column, preparation method and application thereof | |
| CN205844066U (en) | The pretreating device of chemical substance detection in a kind of saliva | |
| CN104330516A (en) | Method for simultaneously determining content of allantoin and adenosine in Chinese yams | |
| CN103983613B (en) | Ractopamine molecular engram spr sensor chip and preparation method thereof | |
| CN103698432A (en) | Method for measuring saponin content in lychee seed extract | |
| CN104237400B (en) | The detection method of content of 5 hydroxymethyl furfural in a kind of prepared rhizome of rehmannia | |
| CN103472144A (en) | Method for rapidly measuring free analyte in biological sample | |
| CN202757793U (en) | Microscale sample moving machine | |
| CN106053648B (en) | A kind of pretreating device of aldehydes matter detection and application | |
| CN102539572A (en) | Method for detecting rutin and quercetol through ionic liquid-accelerating solvent extraction and high performance liquid chromatograph chemiluminescence | |
| CN105866309A (en) | Determination method of vitamin A and vitamin E | |
| CN101963599B (en) | Method for measuring main effective ingredient content of psoralen in cigarettes and main-flow flue gas particle-phase matter | |
| CN102128897A (en) | Ultrasound-assisted emulsification-microextraction method for pretreatment of artificially synthesized musk in water body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |