JP2003014733A - Saliva sample preparation set - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a low-cost and disposable saliva sample preparation set where patients can gather their saliva safely. SOLUTION: The saliva sample preparation set 10 includes a saliva absorption gathering tool 1, and a centrifugal tube 2 for saliva separation collection. The saliva absorption gathering tool 1 includes a treatment rod 12, a saliva absorption body 11 that can be carried at the tip carrier section of the treatment rod detachably, and a pushout means 13 for disconnecting the saliva absorption body from the treatment rod. The treatment rod includes a tip carrier section that can carry the saliva absorption body detachably, and an operation region. The operation region includes the retention and grip sections of the push-out means. In the centrifugal tube for saliva separation collection, a saliva absorption body accommodation chamber 24 being classified by a classification means 22, and a saliva collection chamber 23 are provided in this order toward a direction from an opening end section to a closing end section.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a saliva sample preparation set. Using the saliva sample preparation set of the present invention, a saliva sample can be collected by a non-invasive method that does not cause pain to the patient. The collected saliva sample is, for example, an antibody against a causative bacterium or virus of an infectious disease, which is an IgA antibody or I contained in saliva.
It can be used for immunological analysis (detection or measurement) of the gG antibody.

[0002]

2. Description of the Related Art When diagnosing a patient suspected of having an infectious disease, it is desired to develop a rapid method for identifying the causative bacterium or virus. For viruses and bacteria that are difficult to culture, these antigens present in the blood, cerebrospinal fluid, urine, etc. of patients and the antibodies present in blood have been tested. However, the non-invasive antibody test using saliva has not been performed in Japan because a practical test method has not been established yet. For example, in the case of a patient suspected of having an infection with Mycobacterium tuberculosis, sputum is collected and non-invasive tests are performed on the patient, such as mycobacterial test, culture test, or genetic test. However, sputum may not be collected in old people and children.

On the other hand, saliva can be easily collected, and a non-invasive test for a patient can be performed by measuring IgA antibody and / or IgG antibody contained in saliva. For example, it is possible to provide information useful for diagnosing tuberculosis as shown in Examples below. In human saliva, IgA was 19 mg / 100 mL and IgG was 1.4 m as the antibody composition of a healthy person.
It was reported that g / 100 mL and IgM contained 0.2 mg / 100 mL, and overwhelmingly secreted IgA.
[Taro Hayakawa and Tatsuo Suda, "Oral Biochemistry," 2nd Edition (1994), Ito Dental Publishing, 174]
page]. Murayama et al. Also reported data comparing human saliva samples using absorbent cotton and comparing the protein content (eg albumin, transferrin, total protein, and IgG and IgA) in serum, saliva, and urine. [Nutrition Research, 19,
979-989 (1999)].

As a method of collecting a saliva sample, a method of stimulating saliva secretion by chewing wax and accumulating saliva in a Spitz tube equipped with a saliva funnel (saliva sample preparation set) is used in a caries preventive test. Has been. Also,
As a simple method for collecting saliva for research purposes, for example, a method of using absorbent cotton or sponge in the patient's mouth using tweezers, or a method of using a saliva suction device is used.

[0005]

However, in the sample collecting method in which absorbent cotton or the like is contained in the patient's mouth by using tweezers or the like, the patient's mouth may be damaged by the tweezers. Further, in the sample collecting method using the saliva suction device, the saliva suction device itself is large-scale, and there is a drawback that the cost becomes high. In addition, when collecting saliva samples of suspected infectious patients, it is necessary to avoid the risk of infection to medical personnel, so a disposable collection device is desirable. Therefore, an object of the present invention is to provide a saliva sample preparation set that enables a patient to collect a saliva sample more safely, and is disposable at low cost.

[0006]

According to the present invention, there is provided (1) (a) a tip holding portion capable of detachably holding a saliva absorber and an operation area, and The operation area is
A processing rod including a holding portion of the pushing means and a gripping portion;
(B) a saliva absorber that can be removably supported on the tip supporting portion of the treatment rod, and (c) a saliva absorber that can be held by the treatment rod and can be detached from the treatment rod. Saliva absorption and collection device including a possible extruding means; and (2) the saliva absorbent accommodating chamber and saliva collecting chamber, which are divided by the dividing means which cannot pass the saliva absorbent but can pass the saliva. Can be solved by a saliva sample preparation set characterized by including a centrifugal tube for saliva separation and collection provided in this order from the open end to the closed end.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION A saliva sample preparation set of the present invention includes (1) a saliva absorption and collection tool and (2) a saliva separation and collection centrifuge tube, and, if desired, a gargling cup, purified water,
Alternatively, it may further include physiological saline. Hereinafter, the saliva sample preparation set of the present invention will be described with reference to the accompanying drawings. FIG. 1 shows a saliva sample preparation set 10 of the present invention.
FIG. 4 is a perspective view showing one aspect of the saliva absorption and collection tool 1 in FIG. 3, and FIG. 3 is a perspective view showing one aspect of the saliva separation and collection centrifugal tube 2 in the saliva sample preparation set 10 of the present invention.

A saliva absorption and collection tool 1 shown in FIG. 1 has a cotton ball 11 as a saliva absorber, a thin cylindrical tube (for example, a thin straw) 12 as a processing rod, and a thick cylindrical tube (for example, as a pushing means) (for example, a thin cylindrical tube). A thick straw) 13 and a stopper 14 which can be provided if desired. The thin cylindrical tube 12
A petal-like projection group 12c as a hooking means is provided on the tip end supporting portion 12a of FIG. 2 and its state is enlarged and shown in FIG. Note that, in FIG. 2, the cotton ball 11 carried by the tip carrying part 12a is omitted.

The petal-like projection group 12c that can be provided at the tip of the tip-supporting portion 12a of the thin cylindrical tube 12 is, for example, a plurality of cuts (for example, 14a in the axial direction, at the tip of the thin cylindrical tube 12). , 14b) into a plurality of divided pieces (for example, 15a, 15b, 15c, 15d, 15
e, 15f) is formed, and the divided pieces are pressed outward and bent to be formed. The hooking means that can be used in the present invention is not particularly limited as long as it has a structure capable of detachably holding a saliva absorber (for example, a cotton ball) in the region of the tip holding portion, For example, 1 provided on the side wall or the end of the tip support
Alternatively, it may be a plurality of protrusions (for example, spike-like protrusions).

One or a plurality of saliva absorbers (for example, cotton balls) can be carried on the tip supporting portion of the thin cylindrical tube. It is preferable to provide through holes or non-through holes in the saliva absorber (for example, a cotton ball) in order to allow the saliva absorber (for example, a cotton ball) to be carried on the tip supporting portion. As shown in FIG. 1, when one cotton ball 11 is carried on the tip carrying part 12a of the thin cylindrical tube 12, a cotton ball 11 having a through hole is used, and It is possible to penetrate the tip supporting portion 12a and hook it by the petaloid projection group 12c at the exit of the through hole. Alternatively, a cotton ball having a non-through hole is used, and the tip supporting portion 1 is provided in the non-through hole of the cotton ball.
2a can be inserted and locked inside the cotton ball by the petaloid projection group 12c. When a plurality of cotton balls are to be carried on the tip supporting portion 12a of the thin cylindrical tube 12, a plurality of cotton balls having a through hole are further used to attach the tip supporting portion 12a to the through hole of the cotton ball. It is also possible to make them penetrate one after another and hook them by the petaloid projection group 12c at the exit of the through hole of the last cotton ball. Alternatively, only the cotton ball to be inserted last may be a cotton ball having a non-through hole, and the cotton ball may be hooked by the petaloid projection group 12b inside the cotton ball.

Further, in the saliva absorption / collection tool 1 shown in FIG. 1, a cotton ball 11 is provided by providing a stopper 14.
Can be prevented from moving inward (in the direction indicated by arrow B) along the axial direction of the thin cylindrical tube 12. Figure 1
The stopper 14 of the embodiment shown in FIG.
A notch 14 that cuts a cylindrical tube (for example, a plastic straw) having the same diameter as that of a flexible material into a short length, and further runs vertically from one end to the other end in the longitudinal direction (axial direction).
It can be created by providing a. In the saliva absorption and collection tool 1 shown in FIG. 1, the stopper 1 is used.
By entwining a part of the fiber forming the cotton ball 11 in the cut portion 14 of 4 and locking the cotton ball 11, the cotton ball 11
Can be prevented from moving. In addition, as a stopper, a saliva absorber (for example, a cotton ball) carried on the tip supporting portion of the treatment rod (for example, a thin cylindrical tube) is provided along the axial direction of the treatment rod (for example, a thin cylindrical tube). One or a plurality of projecting pieces having a structure that does not move inward (in the direction indicated by arrow B in FIG. 1) on the side surface of the processing rod (for example, a thin cylindrical tube) directly or through an arbitrary carrier, It can also be provided.

The thick cylindrical tube 13 has an inner diameter larger than the outer diameter of the thin cylindrical tube 12 and the outer diameter of the stopper 14, and can be freely moved along the axial direction of the thin cylindrical tube 12. The thin cylindrical tube 12 includes a tip supporting portion 12a, a gripping portion 12b for an operator to grip the processing rod with his / her hand, and a thick cylindrical tube 13
And a holding unit for holding. The holding portion in the thin cylindrical tube 12 of the embodiment shown in FIG. 1 is a portion between the tip carrying portion 12a and the gripping portion 12b, and is a portion covered with the thick cylindrical tube 13.

The saliva absorber that can be used in the present invention can absorb saliva in the oral cavity by contacting it with the tongue or the tongue, and after absorbing saliva, centrifuge the saliva absorber by centrifugation. The material and structure thereof are not particularly limited as long as saliva can be separated.
As the saliva absorber, for example, a saliva absorber made of a liquid absorbent material (for example, fiber material, particularly cotton or wool) or a liquid absorbent structure (for example, a porous structure such as foam) is used. Examples thereof include saliva absorbers. Further, the shape and size of the saliva absorber are not particularly limited as long as they can be contained in the mouth, and the shape thereof can also be roughly spherical or roughly elliptical. A preferred saliva absorber is a cotton ball made of absorbent cotton.

The treatment rod which can be used in the present invention has a tip supporting portion capable of detachably supporting the saliva absorber at one end, and includes an operation region at the other end side, It is not particularly limited. In the operation area,
A grip portion for an operator to manually handle the processing rod and a holding portion for the pushing means are included. The shape of the treatment rod may be, for example, a cylindrical shape (for example, a cylindrical shape or a polygonal cylindrical shape) or a columnar shape (a cylindrical shape or a polygonal cylindrical shape). The shape of the tip-supporting part of the processing rod is also such that the saliva absorber does not separate from the tip-supporting part when saliva is absorbed and collected, and the saliva absorber is transferred to the accommodating chamber of the saliva separating and collecting centrifuge tube. At this time, it is not particularly limited as long as the saliva absorber can be removed. The material of the treatment rod is preferably non-absorbent of liquid and can be, for example, plastic or metal.

The extrusion means that can be used in the present invention are:
The saliva absorber supported on the tip supporting portion of the treatment rod is particularly limited as long as it can be detached from the treatment rod by extruding it in the outer direction (direction indicated by arrow A) along the axial direction of the treatment rod. However, for example, as shown in FIG. 1, an extruding cylindrical body provided along the outer periphery of the processing rod and movable in the longitudinal direction of the processing rod, or the inside of the cylindrical processing rod. An extruding piston having a pressing member that is provided at the end of the cylindrical processing rod and that is movable in the length direction of the processing rod and that projects to the outside of the cylindrical processing rod at the end on the tip supporting portion side.

When an extruding tubular body is used as the extruding means, it is necessary to make its inner diameter larger than the outer diameter of the processing rod so that the extruding tubular body can move in the axial direction of the processing rod. There is. When the cylindrical stopper is provided, the inner diameter of the extrusion tubular body is made larger than the outer diameter thereof. When a stopper with a protruding portion is provided on a part of the side wall of the processing rod as the stopper, the inner diameter is set so that it can move in the axial direction beyond the protruding portion. A slit portion that exposes a part of the protruding portion to the outside of the extruding tubular body may be provided on a part of the side wall of the body to enable movement in the axial direction. Further, when one end of the extruding tubular body is brought into contact with the saliva absorber as it is to push out the saliva absorber, the outer diameter of the end needs to be smaller than the outer diameter of the saliva absorber. . It is also possible to separately provide a pressing member at the end where the extruding tubular body comes into contact with the saliva absorber and separate the saliva absorber by the pressing member.
The length of the extruding cylinder is as long as the end on the side opposite to the saliva absorber-supporting end of the treatment rod is exposed as a grip during saliva collection (that is, the extruding cylinder is not covered). It is not particularly limited.

The saliva separating and collecting centrifuge tube 2 shown in FIG. 3 has a plastic tube 21 and a holding table 22 as a dividing means. With the holding table 22, the saliva absorber storage chamber 23 and the saliva collecting chamber 24 are provided. It is divided into and. The holding table 22 shown in FIG. 3 is, for example, a groove 22 that can be fitted to each other.
It can be formed by combining a pair of flat plates 22a and 22b having a'and 22b '. The flat plates 22a and 22b in the state before combination are respectively shown in FIG.
It shows in (a) and FIG.4 (b). Each flat plate 22 shown in FIG.
a and 22b are two adjacent rectangular flat plates.
Grooves 22a 'or 22 that can be fitted so that the four corners cut off are aligned in the same plane direction.
b'is provided. On the other hand, since the bottom of the plastic tube 21 has an inverted conical shape in which the inner diameter becomes narrower toward the end, the holding table 22 makes the surface formed by the cut-off square contact with the bottom of the inverted conical shape of the plastic tube 21. Plastic tube 2
It is located within 1.

The centrifuge tube for separation and recovery of saliva that can be used in the present invention is particularly preferable as long as it is a centrifuge tube capable of performing a centrifugation operation sufficient to separate saliva from a saliva absorber that has absorbed saliva. It is not limited, and it can be prepared by inserting the above-mentioned holding stand into a normal centrifuge tube.

The dividing means that can be used in the present invention is
When the saliva absorber is centrifuged in a centrifuge tube for separating and collecting saliva, the saliva absorber cannot pass through, but is not particularly limited as long as saliva can pass through. As the dividing means, for example, FIG.
Examples thereof include a holding table as shown in, or a flat net plate. In the present invention, the dividing means can be disassembled (that is, removable) with the centrifuge tube for saliva separation and collection.
It may be arranged in any state, or the dividing means and the saliva separating and collecting centrifuge tube may be integrally formed.

When the saliva sample preparation set of the present invention including (1) a saliva absorption and collection tool and (2) a saliva separation and collection centrifuge tube is used, it is not particularly limited.
Saliva samples can be collected according to the following procedure. After the saliva sample recipient has gargled with water (eg, purified water) as desired, the saliva absorber (eg, cotton ball) of the saliva absorption / collection device is inserted between the inside of the gums and the tongue, and Until the saliva absorber gets wet (for example, 5 minutes ~
Wait 20 minutes). Then, as shown in step A of FIG.
With the saliva absorber 11 portion of the saliva absorption / collection tool 1 inserted in the saliva separation / collection centrifuge tube 2, the extrusion means 13 of the saliva absorption / collection tool 1 is pushed downward (direction indicated by arrow A). As a result, as shown in step B of FIG. 5, only the saliva absorber 11 is stored in the chamber 2 of the centrifuge tube 2 for saliva separation and recovery.
4 (that is, above the sorting means 22). By centrifuging the saliva separating and collecting centrifuge tube 2 (for example, 3000 rpm for 5 minutes), the saliva absorbed in the saliva absorber 11 becomes saliva collecting chamber 2 of the saliva separating and collecting centrifuge tube 2.
Since the saliva is collected in No. 3, the saliva can be collected by an appropriate suction means such as a tip or a Pasteur pipette to prepare a saliva sample. The saliva sample thus obtained can be used as it is for analysis, or can be stored until it is used for analysis. If desired, it can be used as a sample for analysis after being diluted with an appropriate buffer solution or the like before analysis.

The saliva sample collected by the saliva sample preparation set of the present invention can be analyzed by various known analysis methods. For example, it can be used in a method of immunologically analyzing (detecting or measuring) IgA antibody or IgG antibody contained in saliva against the causative bacterium or virus of an infectious disease. More specifically, for example, Mycobacterium tuberculosis A6
Immunohistochemical analysis of A60 antigen-specific IgA antibody and / or IgG antibody contained in a saliva sample using 0 antigen to determine the presence or absence of infection with Mycobacterium tuberculosis, that is, an antibody for diagnosing tuberculosis. The inspection can be performed quickly.

[0022]

The present invention will be described in detail below with reference to examples, but these do not limit the scope of the present invention.

Example 1 << Saliva Collection >> In this example, a saliva absorption collection tool 1 shown in FIG. 1 and a saliva separation / collection centrifuge tube 2 shown in FIG. 3 (the flat plates 22a and 22b shown in FIG. 4 as a dividing means).
The saliva sample preparation set of the present invention including a holding table 22), and saliva samples were collected from 6 patients eradicating Mycobacterium tuberculosis by the following procedure. The dimensions of the saliva absorption and sampling tool 1 are as shown in FIG.
Is 125 mm, b is 55 mm, c is 10 m
It was m. The diameter of the cotton ball (Kawamoto Sangyo) 11 is 10
mm, the diameter of the thin cylindrical tube 12 was 3 mm, and the diameter of the thick cylindrical tube 13 was 5 mm. Further, in FIG. 4, the respective dimensions of the flat plates 22a and 22b are d = 12 mm, e = 16 mm, f = 12 mm, and g = 1.
It was 6 mm. A 15 mL centrifuge tube was used as the plastic tube 21.

First, after gargle with purified water (about 10 mL), put a cotton ball between the tongue and the inside of the gums and wait until the cotton ball gets wet with saliva (about 10 minutes) to absorb saliva. .
Only the cotton balls were transferred to a centrifuge tube for saliva separation and collection, and centrifugation was performed at 3,000 rpm for 5 minutes using a centrifuge.
The saliva sample obtained by separating the bottom solution with a Pasteur pipette is used at −20 ° C. until it is used in Example 2 described later.
Saved in.

[0024]

Example 2 << Antibody in saliva using A60 antigen (Ig
A and IgG) >> Antibodies (IgA and IgG) can be measured by M. tuberculosis A60 antibody test kit [AndaBioc
chemicals (France)] was used. The saliva sample collected in Example 1 was treated with phosphate buffered saline [PBS; 10 mmol / L phosphate buffer (pH
7.4) and 0.15 mol / L sodium chloride]
After diluting 0 times, 100 μl of the diluted solution was added to the IgA antibody measurement microplate and the IgG antibody measurement microplate, and the mixture was reacted at 37 ° C. for 1 hour. After completion of the reaction, the cells were washed 5 times with a washing buffer [PBS containing 1% bovine serum albumin (BSA) and 0.2% Tween 20] using a washing device (DIA-WASHERII device; Dyatron).

Next, as a secondary antibody, a peroxidase-labeled anti-human IgA monoclonal antibody or a peroxidase-labeled anti-human IgG monoclonal antibody was added, and 3
After reacting for 30 minutes at 7 ° C., it was washed 5 times with the above-mentioned washing buffer. Subsequently, the substrate solution (Tetra-Met
After adding hyl-Benzidine and 0.02% H ₂ O ₂ ) and reacting at 37 ° C. for 15 minutes, a reaction stop solution (0.5N sulfuric acid) was added to stop the reaction. This color is developed by a microplate reader (MPR-A4i device;
Tosoh) was used to measure the absorbance at 450 nm. The amount of IgA antibody and the amount of IgG antibody are the standard products of IgA antibody and IgG antibody (1 U / mL, 2 U / m, respectively).
L, 4 U / mL, 8 U / mL, 16 U / mL).

The results are shown in Table 1 together with the result (Gaffkey number) of Reference Example 1 described later. Ig in saliva in Table 1
In the determination of A antibody, the symbol “+” means that the amount of IgA antibody in saliva exceeds 10 U / mL, and the symbol “±”.
Means 5 to 10 U / mL, and the symbol "-"
Means less than 5 U / mL. Also, Table 1
"+" In the determination of IgG antibody in saliva in
Means that the IgG antibody amount in saliva is 5 U / mL or more, and the symbol "-" means less than 5 U / mL. When saliva collected from 6 patients with Mycobacterium tuberculosis same as in Example 1 was used as a test material and the antibody titer of IgA antibody or IgG antibody that reacts with A60 antigen was confirmed, as shown in Table 1, the IgA antibody in saliva was shown. It is clear that there is a patient (patient No. 2, patient No. 3, and patient No. 6) whose serum level is elevated, and a patient (patient No. 1) whose IgG antibody in saliva is elevated. Became.

<< Table 1 >> Patient Gaffky Saliva IgA antibody in saliva IgG antibody number in saliva U / mL judgment U / mL judgment 1 10 9.8 ± 9.2 ± 24 47.8 + 0.0 − 33 No. 75.2 + 0.0-43 No. 5.0 ± 0.0-5 54 No. 7.0 ± 0.0-6 No. 6 92.6 + 0.0-

[0028]

[Reference Example 1] << Confirmation of presence / absence of bacillus of tuberculosis patient >> The presence / absence of bacillus (the presence of tubercle bacillus in sputum) of the 6 patients who collected saliva in Example 1 was determined by the Gaffkey number. (0
No. to No. 10). That is, a sputum collected from each patient was applied to a slide glass, naturally dried, and then slowly passed through a flame with the smear surface facing upward for 2-3 times to fix the specimen, thereby preparing a sample. The acid-fast bacilli were stained in the above specimens by the Ciel-Nelsen method using fuchsine carbonate and methylene blue.
As a result of the above staining, the acid-fast bacterium stains red, and other cells and general bacteria stain blue.
The number of mycobacteria was examined by the number of times, and the number was indicated by the number of Gaffkey [Kanai Publishing Co.
Page 1112]. The results are shown in Table 1.

[0029]

[Reference Example 2] << Antibodies (Ig
A and IgG) >> Antibodies against A60 protein complex (IgA), which is a constituent of Mycobacterium tuberculosis cell protein
Or IgG) has been found to be present in the blood of patients with tuberculosis infection [Klin. Wochensch
r. , 69, 696-709 (1991), and Med.
ical Hypotheses, 52, 583-59
3 (1999)]. In this reference example, the amount of antibody in the blood collected from the 6 patients who collected saliva in Example 1 was measured. That is, the patient 6 who collected saliva in Example 1.
From the name, after collecting blood, centrifugation (3,000 rp
m × 10 min) to obtain serum. After diluting this serum 100 times with PBS, IgA antibody and IgG antibody were measured by the same operation as in Example 2 using 100 μl of each of the diluted solutions. As for serum, standard products of IgA antibody and IgG antibody (1 U / m) were used.
L, 2U / mL, 4U / mL, 8U / mL, 16U / m
L) was used for conversion.

The results are shown in Table 2 together with the results of the above-mentioned Reference Example 1 (Gaffkey number). Serum IgA in Table 2
In the determination of the antibody, the symbol “+” means that the amount of IgA antibody in saliva exceeds 350 U / mL, and the symbol “±”.
Means 200 to 350 U / mL, and the symbol "-" means less than 200 U / mL.
Further, in the determination of serum IgG antibody in Table 2, the symbol “+” means that the amount of IgG antibody in saliva exceeds 225 U / mL, and the symbol “±” indicates 125 to 225 U / mL.
It means that it is mL, and the symbol "-" is 125 U / m.
It is less than L.

<< Table 2 >> Patient Gaffy serum IgA antibody Serum IgG antibody number No. U / mL judgment U / mL judgment 1 10 488 + 2562 + 24 4 162-158 ± 33 3 276 ± 182 ± 43 475 + 274 + 54 No. 231 ± 624 + 66 No. 185-1259 +

[0032]

According to the saliva sample preparation set of the present invention, saliva can be collected at low cost and more safely for the patient. Moreover, since it can be disposable, the risk of infection to medical personnel can be avoided.

[Brief description of drawings]

FIG. 1 is a perspective view showing one embodiment of a saliva absorption and collection tool in a saliva sample preparation set of the present invention.

FIG. 2 is an enlarged perspective view showing a leading end carrying portion of the thin cylindrical tube shown in FIG.

FIG. 3 is a perspective view showing one embodiment of a centrifuge tube for saliva separation and collection in the saliva sample preparation set of the present invention.

FIG. 4 is an exploded perspective view of the holding table shown in FIG.

FIG. 5 is an explanatory view showing a part of a saliva collecting step using the saliva sample preparation set of the present invention.

[Explanation of symbols]

10 ... Saliva sample preparation set; 1 ... Saliva absorption and collection tool; 2 ... Saliva separation and collection centrifuge tube; 11 ...
Saliva absorber; 12 ... Thin cylindrical tube; 13 ... Thick cylindrical tube; 14 ... Stopper; 21 ... Plastic tube; 22 ... Holding table; 23 ... Saliva absorber storage chamber; 24 ... Saliva recovery room.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 1/10 G01N 1/10 V 33/50 33/50 G 33/53 33/53 N F term (reference) ) 2G045 BB10 BB11 CB07 CB21 DA37 HA07 HA14 JA07 2G052 AA29 AA36 AB18 AD06 AD26 AD46 BA02 BA14 CA02 CA18 CA32 CA45 DA02 DA27 DA33 EC00 ED06 ED09 ED17 FA08 FD01 GA12 GA12 GA30 JA05 JA08 JA13 JA15 JA16 JA23 A26 JA26 JA23 A26 JA20 JA20

Claims (3)

[Claims] (1) (a) A tip holding portion capable of detachably holding a saliva absorber and an operation area, wherein the operation area includes a holding portion of the pushing means and a grip portion. And (b) a saliva absorber that can be detachably supported on the tip supporting portion of the treatment rod, and (c) a saliva absorber that can be held by the treatment rod, and the saliva absorber can be treated as described above. A saliva absorption / collection device including an extruding means that can be detached from the rod; and (2) a saliva absorbent divided by a dividing means that cannot pass the saliva absorbent but a saliva can pass through. A saliva sample preparation set, characterized in that the storage chamber and the saliva recovery chamber include centrifugal tubes for saliva separation and recovery, which are provided in this order in the direction from the open end to the closed end. 2. The extruding means that can be held by the processing rod is a cylindrical body that is provided along the outer periphery of the operation region of the processing rod and that can reciprocate in the longitudinal direction of the processing rod.
The saliva sample preparation set according to claim 1. 3. The saliva sample preparation set according to claim 1, wherein the saliva absorber 2 or more is carried on the tip carrying part of the treatment rod.