CN104211831B - A kind of preparation method of hypo-methoxy pectin - Google Patents
A kind of preparation method of hypo-methoxy pectin Download PDFInfo
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- 229920001277 pectin Polymers 0.000 title claims abstract description 60
- 235000010987 pectin Nutrition 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 239000012535 impurity Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000002244 precipitate Substances 0.000 claims abstract description 13
- 239000000047 product Substances 0.000 claims abstract description 13
- 235000003222 Helianthus annuus Nutrition 0.000 claims abstract description 12
- 238000005406 washing Methods 0.000 claims abstract description 12
- 239000000284 extract Substances 0.000 claims abstract description 11
- VBIXEXWLHSRNKB-UHFFFAOYSA-N Ammonium oxalate Chemical compound [NH4+].[NH4+].[O-]C(=O)C([O-])=O VBIXEXWLHSRNKB-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 claims abstract description 8
- 238000000926 separation method Methods 0.000 claims abstract description 6
- 229940059442 hemicellulase Drugs 0.000 claims abstract 2
- 108010002430 hemicellulase Proteins 0.000 claims abstract 2
- 239000000843 powder Substances 0.000 claims description 16
- 238000001556 precipitation Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N HCl Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 239000006227 byproduct Substances 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 229940106157 CELLULASE Drugs 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 238000005516 engineering process Methods 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000000919 ceramic Substances 0.000 claims description 4
- 230000001186 cumulative Effects 0.000 claims description 4
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- 229940079593 drugs Drugs 0.000 claims description 4
- 230000001105 regulatory Effects 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 239000001117 sulphuric acid Substances 0.000 claims description 4
- 235000011149 sulphuric acid Nutrition 0.000 claims description 4
- 235000013311 vegetables Nutrition 0.000 claims description 4
- 150000002118 epoxides Chemical class 0.000 claims description 2
- 240000006669 Helianthus annuus Species 0.000 claims 1
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- 238000011017 operating method Methods 0.000 abstract description 2
- 238000007605 air drying Methods 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 150000004676 glycans Polymers 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000000049 pigment Substances 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 150000004804 polysaccharides Polymers 0.000 abstract 1
- 241000282898 Sus scrofa Species 0.000 description 8
- 210000000582 Semen Anatomy 0.000 description 7
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 6
- 229940074393 Chlorogenic acid Drugs 0.000 description 6
- CWVRJTMFETXNAD-JUHZACGLSA-N Chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 6
- 240000005158 Phaseolus vulgaris Species 0.000 description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 6
- 240000006394 Sorghum bicolor Species 0.000 description 6
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- 229940046009 Vitamin E Drugs 0.000 description 6
- 229930003427 Vitamin E Natural products 0.000 description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L Zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 6
- 235000001368 chlorogenic acid Nutrition 0.000 description 6
- GRVDJDISBSALJP-UHFFFAOYSA-N methyloxidanyl Chemical group [O]C GRVDJDISBSALJP-UHFFFAOYSA-N 0.000 description 6
- 235000019165 vitamin E Nutrition 0.000 description 6
- 239000011709 vitamin E Substances 0.000 description 6
- 150000003712 vitamin E derivatives Chemical class 0.000 description 6
- 229960001763 zinc sulfate Drugs 0.000 description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- -1 enzymolysis Substances 0.000 description 4
- 238000004821 distillation Methods 0.000 description 3
- 238000005886 esterification reaction Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 235000011837 pasties Nutrition 0.000 description 3
- 238000010792 warming Methods 0.000 description 3
- 210000002421 Cell Wall Anatomy 0.000 description 2
- AEMOLEFTQBMNLQ-YMDCURPLSA-N D-Galacturonic acid Chemical compound OC1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-YMDCURPLSA-N 0.000 description 2
- AEMOLEFTQBMNLQ-DTEWXJGMSA-N D-Galacturonic acid Natural products O[C@@H]1O[C@H](C(O)=O)[C@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-DTEWXJGMSA-N 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 2
- 229910001424 calcium ion Inorganic materials 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
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- 238000001879 gelation Methods 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- MUBZPKHOEPUJKR-UHFFFAOYSA-L oxalate Chemical compound [O-]C(=O)C([O-])=O MUBZPKHOEPUJKR-UHFFFAOYSA-L 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
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- 241001301148 Brassica rapa subsp. oleifera Species 0.000 description 1
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- 238000005265 energy consumption Methods 0.000 description 1
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- 230000035611 feeding Effects 0.000 description 1
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Abstract
The present invention relates to a kind of method that hypo-methoxy pectin is prepared in extraction from sunflower plate, with the sunflower plate after air-drying as raw material, after size-reduced 60 mesh standard sieves sieve, carry out pretreatment and remove pigment, impurity and soluble polysaccharide；After being washed to clearly, the solid-liquid ratio by 1: 30 adds extracting solution, adds appropriate cellulose and hemicellulase Hydrolysis At Room Temperature 30min in extracting solution；The pH of regulation extracting solution is 3.0, adds the extraction of appropriate ammonium oxalate, obtains pectin extracting soln after solid-liquid separation；Extracting solution concentrates after plate-and-frame filtration remove impurity；Concentrated solution adds acidifying alcohol and carries out alcohol analysis, precipitate 2～3 times by washing with alcohol after filtration, be vacuum dried to obtain pectin product.It is a feature of the present invention that operating procedure is simple, production cost is low, and the safety of product is good, extracts yield and purity is high, be suitable to industrialized production.
Description
Technical field
The invention belongs to medicine and food industrial technical field, be specifically related to the preparation method of a kind of hypo-methoxy pectin.
Background technology
1825, Frenchman Bracennot extracted a kind of material first from Radix Dauci Sativae meat, and this material can be formed solidifying
Glue, Bracennot by named for its extract " pectin ", in referred to herein as " pectin ".Pectin is that a kind of hydrophilic is planted
Thing glue, its main component is D-galacturonic acid, and the World Food Programme (FAO) and European Union (EU) regulation, pectin must
Must be widely present in green terrestrial plant intercellular substance containing the galacturonic acid of >=65%, fix water by its gelation
Point, there is together with fiber the effect combining plant tissue.Pectin is the main component of dietary fiber in human body seven major nutrient,
There is good diarrhea, anticancer, treatment effect such as diabetes and fat-reducing, and pectin has good gelation and emulsifying is steady
Qualitative, it is widely used in the food industry, is also medicine and the indispensable adjuvant of cosmetics industry.Esterification degree pressed by commodity pectin
The percentage rate of total hydroxyl value (the esterified hydroxy groups number account for) difference can be divided into two big classes, low methoxyl pectin (hypo-methoxy pectin, Low
Methoxyl Pectin, LMP, methoxyl content is less than 7%, i.e. esterification degree is less than 42.9) and high-esterpectin (high methoxyl fruit
Glue, High Methoxyl Pectin, HMP, methoxyl content is more than 7%, i.e. esterification degree is more than 42.9).
The extraction of pectin currently mainly has acid extraction method, ion-exchange-resin process, enzymatic isolation method, microwave-assisted extraction and micro-life
Thing method etc..The oxalate denominationby of ammonium oxalate has used by the strongest metal-chelating, can will chelate with calcium ion in plant cell wall
The water-insoluble pectin extraction formed is out.Further, since ammonium oxalate is a kind of alkalescence salt, to the pollution of environment well below
Strong acid and strong base, in actual production, the corrosion to equipment to be far smaller than tradition acid system, and therefore, available ammonium oxalate is as extractant
Industrial abstract pectin.
Helianthi and Semen sojae atricolor, Brassica campestris L, Semen arachidis hypogaeae, Semen Sesami are the big oil crop of China five.Helianthi has that period of duration is short, high yield,
The good characteristics such as high-quality, low cost, Salt And Alkali Tolerance, drought-resistant, wide adaptability, still can obtain under conditions of cultivation step is extensive
Preferably harvest.Helianthi Potential Comprehensive Utilization is big, in addition to Semen Helianthi extracts oil, goes the pectin content in the sunflower plate of seed high
Reach 17%～25%, be good natural hypo-methoxy pectin source.
Summary of the invention
It is an object of the invention to provide a kind of high-efficiency environment friendly, simple, cost is relatively low, is suitable to a kind of low first of industrialized production
The preparation method of epoxide pectin.Using tradition acidity extraction pectin, not only extraction efficiency is low, and equipment corrosion-vulnerable, to ring
Environment pollution is relatively big, and the oxalate denominationby of ammonium oxalate has used by the strongest metal-chelating, can by plant cell wall with calcium ion
The water-insoluble pectin extraction that chelating is formed out, the invention provides the preparation method of a kind of hypo-methoxy pectin.
The invention provides the preparation method of a kind of hypo-methoxy pectin, the method comprise the steps: pretreatment of raw material, enzymolysis,
Extract, filter, decolour, concentrate, precipitate, wash, drying and crushing and side-product processing；
It specifically includes:
(1) pretreatment of raw material: choose air-dry after remove seed sunflower plate, with vegetable drug pulverizer pulverize after, cross 60 mesh standard sieves；
Weigh sunflower plate powder 30g to be placed in container, be under conditions of 10.0 temperature are 70 DEG C at pH, soak 15-20min, then
200 turns/min is centrifuged 5 minutes, collects precipitation and liquid；
(2) enzymolysis: add deionized water 900ml in step (1) gained precipitates, is added thereto to 100U cellulase and 60U half
Cellulase, is then 4.5-5.0 with the salt acid for adjusting pH value of 1M, room temperature enzymolysis 30min；
(3) extraction: the pH of the enzymolysis solution obtained with the sulphuric acid regulating step (2) of 1M is 3.0, is subsequently adding ammonium oxalate 7.2g, has treated
After CL, under the conditions of temperature is 85 DEG C, stirring in water bath extracts 4h；
(4) filter: extracting solution step (3) obtained is centrifuged 3 minutes through 4000 turns/min and carries out solid-liquid separation, collect supernatant,
Supernatant collects filtrate and impurity through plate-and-frame filtration；
(5) decolouring: adding activated carbon 7g in step (4) gained filtrate, then regulation pH value is 7.0, is 50 in temperature
Being incubated 30min under the conditions of DEG C and carry out desolventing technology, then filtering and impurity removing obtains pectin extracting soln；
(6) concentrate: the pectin extracting soln after decolouring is concentrated into the 1/3 of original solution volume through ceramic membrane ultrafitration, obtains concentrated solution；
(7) precipitation: add the hydrochloric acid of the 1M of account for concentrated solution cumulative volume 1% in concentrated solution, add isopyknic with concentrated solution 95%
(V/V) ethanol, at room temperature after precipitation 4h, is collected by filtration precipitation；
(8) washing: add 80% (V/V) washing with alcohol 2 times of two volumes in gained precipitates, through screw press after washing,
Remove unnecessary alcohol and water, obtain wet product pectin；
(9) drying and crushing: by step (8) gained wet product pectin at 60-65 DEG C, is dried 12-15h, after pulverizing under the conditions of 0.17Mpa
Pack and i.e. obtain hypo-methoxy pectin；
(10) side-product processing: step (1) gained liquid and step (4) gained impurity are mixed to get complex A, the most past
Adding Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici in complex A, interpolation limit, limit is stirred to pasty state；Finally it is passed through steam to be warming up to
110 DEG C, distill 15 minutes；Then, after distillation drying, pulverizing, zinc sulfate, vitamin E and chlorogenic acid, mixing are added
Uniformly, it is thus achieved that pig feed；Wherein, Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici account for the 7% of complex A mass respectively, and 6%, 3
% and 2%, zinc sulfate, vitamin E and chlorogenic acid account for respectively complex A mass ten thousand/.
Compared with existing pectous extracting method technology, advantages of the present invention specifically includes that
(1) use ammonium oxalate extraction process, compared with tradition acid system, not only reduce the corrosion to equipment, it is often more important that it is right to be
The protection of environment；
(2) method carrying out enzymolysis by addition cellulose and hemicellulose compound enzyme, the release of the most intracellular pectin substance, from
And effectively raise pectin extraction rate；
(3) compared with traditional vacuum concentration method, inoranic membrane separation ultrafiltration concentration technology is used not only to effectively reduce energy consumption, Er Qieqi
Operating procedure is simple, can remove the sugar impurity in pectin extracting soln and oligomer simultaneously, thus improve the quality of pectin, also
Without heating, to pectin quality not damaged；
(4) owing to the present invention is with the sunflower plate of main producing region, Xinjiang as raw material, go the pectin content in the sunflower plate of seed be up to 17%～
25%, it is good natural hypo-methoxy pectin source, by enzymolysis and ammonium oxalate compound collecting, it is possible to effectively reduce and produce into
This, product meets the World Food Programme (FAO) and European Union (EU) regulation, also complies with the food of China GB 25533-2010
The standard of product additive pectin, it is possible to be directly entered market as products in circulation.
(5) present invention efficiently utilizes refuse, is prepared for pig feed, it is achieved that turn waste into wealth.
Detailed description of the invention
Based on above extracting method, below technical solution of the present invention concrete operations are described in detail, but the protection model of the present invention
Enclose and be not limited solely to described example, simply the present invention is further illustrated.
Embodiment 1
A kind of preparation method of hypo-methoxy pectin, the method comprise the steps: pretreatment of raw material, enzymolysis, extract, filter,
Decolour, concentrate, precipitate, wash, drying and crushing and side-product processing；
(1) pretreatment of raw material: choose air-dry after remove seed sunflower plate, with vegetable drug pulverizer pulverize after, cross 60 mesh standard sieves；
Weigh sunflower plate powder 30g to be placed in container, be under conditions of 10.0 temperature are 70 DEG C at pH, soak 15min, then 200
Turn/min is centrifuged 5 minutes, collects precipitation and liquid；
(2) enzymolysis: add deionized water 900ml in step (1) gained precipitates, is added thereto to 100U cellulase and 60U half
Cellulase, is then 4.5 with the salt acid for adjusting pH value of 1M, room temperature enzymolysis 30min；
(3) extraction: the pH of the enzymolysis solution obtained with the sulphuric acid regulating step (2) of 1M is 3.0, is subsequently adding ammonium oxalate 7.2g, has treated
After CL, under the conditions of temperature is 85 DEG C, stirring in water bath extracts 4h；
(4) filter: extracting solution step (3) obtained is centrifuged 3 minutes through 4000 turns/min and carries out solid-liquid separation, collect supernatant,
Supernatant collects filtrate and impurity through plate-and-frame filtration；
(5) decolouring: adding activated carbon 7g in step (4) gained filtrate, then regulation pH value is 7.0, is 50 in temperature
Being incubated 30min under the conditions of DEG C and carry out desolventing technology, then filtering and impurity removing obtains pectin extracting soln；
(6) concentrate: the pectin extracting soln after decolouring is concentrated into the 1/3 of original solution volume through ceramic membrane ultrafitration, obtains concentrated solution；
(7) precipitation: add the hydrochloric acid of the 1M of account for concentrated solution cumulative volume 1% in concentrated solution, add isopyknic with concentrated solution 95%
(V/V) ethanol, at room temperature after precipitation 4h, is collected by filtration precipitation；
(8) washing: add 80% (V/V) washing with alcohol 2 times of two volumes in gained precipitates, through screw press after washing,
Remove unnecessary alcohol and water, obtain wet product pectin；
(9) drying and crushing: by step (8) gained wet product pectin at 60 DEG C, is dried 12h under the conditions of 0.17Mpa, after pulverizing, packaging is i.e.
Obtain hypo-methoxy pectin；
(10) side-product processing: step (1) gained liquid and step (4) gained impurity are mixed to get complex A, the most past
Adding Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici in complex A, interpolation limit, limit is stirred to pasty state；Finally it is passed through steam to be warming up to
110 DEG C, distill 15 minutes；Then, after distillation drying, pulverizing, zinc sulfate, vitamin E and chlorogenic acid, mixing are added
Uniformly, it is thus achieved that pig feed；Wherein, Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici account for the 7% of complex A mass respectively, and 6%, 3
% and 2%, zinc sulfate, vitamin E and chlorogenic acid account for respectively complex A mass ten thousand/.
After testing, extraction hypo-methoxy pectin is 3.69kg, and extraction ratio is 12.3%, and it is 88.7 that galacturonic acid acid system measures purity
%.
Embodiment 2
A kind of preparation method of hypo-methoxy pectin, the method comprise the steps: pretreatment of raw material, enzymolysis, extract, filter,
Decolour, concentrate, precipitate, wash, drying and crushing and side-product processing；
(1) pretreatment of raw material: choose air-dry after remove seed sunflower plate, with vegetable drug pulverizer pulverize after, cross 60 mesh standard sieves；
Weigh sunflower plate powder 30g to be placed in container, be under conditions of 10.0 temperature are 70 DEG C at pH, soak 20min, then 200
Turn/min is centrifuged 5 minutes, collects precipitation and liquid；
(2) enzymolysis: add deionized water 900ml in step (1) gained precipitates, is added thereto to 100U cellulase and 60U half
Cellulase, is then 5.0 with the salt acid for adjusting pH value of 1M, room temperature enzymolysis 30min；
(3) extraction: the pH of the enzymolysis solution obtained with the sulphuric acid regulating step (2) of 1M is 3.0, is subsequently adding ammonium oxalate 7.2g, has treated
After CL, under the conditions of temperature is 85 DEG C, stirring in water bath extracts 4h；
(4) filter: extracting solution step (3) obtained is centrifuged 3 minutes through 4000 turns/min and carries out solid-liquid separation, collect supernatant,
Supernatant collects filtrate and impurity through plate-and-frame filtration；
(5) decolouring: adding activated carbon 7g in step (4) gained filtrate, then regulation pH value is 7.0, is 50 in temperature
Being incubated 30min under the conditions of DEG C and carry out desolventing technology, then filtering and impurity removing obtains pectin extracting soln；
(6) concentrate: the pectin extracting soln after decolouring is concentrated into the 1/3 of original solution volume through ceramic membrane ultrafitration, obtains concentrated solution；
(7) precipitation: add the hydrochloric acid of the 1M of account for concentrated solution cumulative volume 1% in concentrated solution, add isopyknic with concentrated solution 95%
(V/V) ethanol, at room temperature after precipitation 4h, is collected by filtration precipitation；
(8) washing: add 80% (V/V) washing with alcohol 2 times of two volumes in gained precipitates, through screw press after washing,
Remove unnecessary alcohol and water, obtain wet product pectin；
(9) drying and crushing: by step (8) gained wet product pectin at 65 DEG C, is dried 15h under the conditions of 0.17Mpa, after pulverizing, packaging is i.e.
Obtain hypo-methoxy pectin；
(10) side-product processing: step (1) gained liquid and step (4) gained impurity are mixed to get complex A, the most past
Adding Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici in complex A, interpolation limit, limit is stirred to pasty state；Finally it is passed through steam to be warming up to
110 DEG C, distill 15 minutes；Then, after distillation drying, pulverizing, zinc sulfate, vitamin E and chlorogenic acid, mixing are added
Uniformly, it is thus achieved that pig feed；Wherein, Semen Maydis powder, Sorghum vulgare Pers. powder, bean dregs and Testa Tritici account for the 7% of complex A mass respectively, and 6%, 3
% and 2%, zinc sulfate, vitamin E and chlorogenic acid account for respectively complex A mass ten thousand/.
After testing, extraction hypo-methoxy pectin is 3.72kg, and extraction ratio is 12.4%, and purity is 88.6%.
Embodiment 3
The performance detection of side-product pig feed of the present invention:
Choosing a month big ablactational baby pig 200, be divided into two groups, often group 100, wherein the experimental group present invention implements
The diet of example 1 preparation, every 50kg about 250 yuan, matched group is with honest feedstuff (SSB-25 model), according to every 50kg
About 300 yuan of calculating.Detect indices after raising 6 weeks and see table 1.
Table 1
| Index (every piglet) | Matched group | Of the present invention group |
| Ablactational baby pig body weight (kg) | 5.37 | 5.42 |
| Within 6 weeks, put on weight (kg) | 12.98 | 13.24 |
| Survive number | 91 | 95 |
| Consume feedstuff (kg) | 15.5 | 16.1 |
| Feed for nursing cost (first) | 93 | 80.5 |
Pig feed cost prepared by the present invention is cheap compared with market common feedstuffs, and feeding effect is good, and the recovery again belonging to refuse should
With, it is achieved that turn waste into wealth, energy-saving and emission-reduction.
Finally, in addition it is also necessary to be only several specific embodiments of the present invention it is noted that listed above.Obviously, the present invention is not
It is limited to above example, it is also possible to have many deformation.Those of ordinary skill in the art directly can lead from present disclosure
The all deformation gone out or associate, are all considered as protection scope of the present invention.
Claims (1)
1. the preparation method of a hypo-methoxy pectin, it is characterised in that described preparation method comprises the steps:
Step (1) pretreatment of raw material: choose air-dry after remove seed sunflower plate, with vegetable drug pulverizer pulverize after, cross 60 mesh standard sieves；Weigh to day
Certain herbaceous plants with big flowers dish powder 30g is placed in container, is under conditions of 10.0 temperature are 70 DEG C at pH, soaks 15-20min, and then 200 turns/min is centrifuged 5 points
Clock, collects precipitation and liquid；
Step (2) enzymolysis: add deionized water 900ml in step (1) gained precipitates, be added thereto to 100U cellulase and 60U hemicellulase,
Then it is 4.5-5.0 with the salt acid for adjusting pH value of 1M, room temperature enzymolysis 30min；
Step (3) extracts: the pH of the enzymolysis solution obtained with the sulphuric acid regulating step (2) of 1M is 3.0, is subsequently adding ammonium oxalate 7.2g, until completely dissolved,
Under the conditions of temperature is 85 DEG C, stirring in water bath extracts 4h；
Step (4) filters: extracting solution step (3) obtained is centrifuged 3 minutes through 4000 turns/min and carries out solid-liquid separation, collects supernatant, and supernatant is through plate
Frame is collected by filtration filtrate and impurity；
Step (5) is decoloured: adding activated carbon 7g in step (4) gained filtrate, then regulation pH value is 7.0, is incubated 30min under the conditions of temperature is 50 DEG C
Carrying out desolventing technology, then filtering and impurity removing obtains pectin extracting soln；
Step (6) concentrates: the pectin extracting soln after decolouring is concentrated into the 1/3 of original solution volume through ceramic membrane ultrafitration, obtains concentrated solution；
Step (7) precipitates: adds the hydrochloric acid of the 1M of account for concentrated solution cumulative volume 1% in concentrated solution, adds 95% (V/V) isopyknic with concentrated solution
Ethanol, at room temperature after precipitation 4h, is collected by filtration precipitation；
Step (8) is washed: adding 80% (V/V) washing with alcohol 2 times of two volumes in gained precipitates, through screw press after washing, it is unnecessary to remove
Second alcohol and water, obtains wet product pectin；
Step (9) drying and crushing: by step (8) gained wet product pectin at 60-65 DEG C, is dried 12-15h, packs and i.e. obtain low first after pulverizing under the conditions of 0.17Mpa
Epoxide pectin；
Step (10) side-product is processed.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034659A (en) * | 1987-12-31 | 1989-08-16 | 云南省轻工业科学研究所 | From papaya, extract edible pectin |
| CN103073657A (en) * | 2013-01-15 | 2013-05-01 | 中北大学 | Method for manufacturing sunflower low-fat pectins |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
| CN103641931A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue |
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034659A (en) * | 1987-12-31 | 1989-08-16 | 云南省轻工业科学研究所 | From papaya, extract edible pectin |
| CN103073657A (en) * | 2013-01-15 | 2013-05-01 | 中北大学 | Method for manufacturing sunflower low-fat pectins |
| CN103641930A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue by enzymatic process |
| CN103641931A (en) * | 2013-11-27 | 2014-03-19 | 威海市桢昊生物技术有限公司 | Method for extraction of pectin from pineapple peels and residue |
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