CN104193519B - Formula of culture medium for Tricholoma matsutake and preparation method of culture medium - Google Patents
Formula of culture medium for Tricholoma matsutake and preparation method of culture medium Download PDFInfo
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- CN104193519B CN104193519B CN201410431909.2A CN201410431909A CN104193519B CN 104193519 B CN104193519 B CN 104193519B CN 201410431909 A CN201410431909 A CN 201410431909A CN 104193519 B CN104193519 B CN 104193519B
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Abstract
The invention relates to the field of edible mushrooms and particularly relates to a formula of a culture medium for Tricholoma matsutake. The formula of the culture medium for Tricholoma matsutake comprises the following raw materials in parts by mass: 30-45 parts of pine sawdust, 5-10 parts of cottonseed hull, 5-10 parts of rapeseed cakes, 5-10 parts of sandy soil, 5-10 parts of pine needles, 5-10 parts of wheat straw, 0.5-1 part of glucose, 0.2-0.3 part of calcium superphosphate and 0.2-0.4 part of magnesium sulfate, wherein the pine sawdust is preferably sawdust of roots of Japanese red pine and/or pinus pumila of which the tree age is above 20 year olds. The preparation method comprises the steps of mixing the pine sawdust, the cottonseed hull, the rapeseed cakes, the pine needles and the wheat straw together, pouring into a pulverizer for pulverizing into particles of which the sizes are 60-100 meshes, then placing into a fermentation tank for soaking in water and retting, airing until the moisture content is 10%-15%, then evenly mixing with sandy soil, glucose, calcium superphosphate and magnesium sulfate and adding 10＜-10＞ mole/liter indole acetic acid solution according to the mass ratio of the culture medium to water being 5 to 1. The formula has balanced and sufficient nutrients and the growth of the Tricholoma matsutake can be significantly accelerated by virtue of the culture medium.
Description
Technical field
The present invention relates to edible mushroom field and in particular to a kind of pine bacterium culture material formula.
Background technology
Loose bacterium, scientific name Trichotoma matsutake, another name pine mushroom, closes bacterium, platform bacterium, is rare famous and precious natural medicinal fungus, two grades of China protection species in imminent danger in the world.There are the areas such as Shangri-la Chan Rong area, Chuxiong Chan Rong area and Yan Bian Chan Rong area in main Chan Rong area of China, and wherein Shangri-la Chan Rong area accounts for the 70% of national total output, is the matsutake outlet champion of continuous 30 years.Matsutake contains 18 kinds of amino acid, 14 kinds of micro elements needed by human, 49 kinds of active nutritional materials, 5 kinds of unrighted acids, 8 kinds of vitamin, 2 kinds of glycoprotein, abundant dietary fiber and various active enzyme, separately containing 3 kinds of precious active materials, it is double-strand blazei polysaccharide, matsutake polypeptide and the whole world unique cancer-resisting substance matsutakealcohol respectively, be natural medical fungus most precious in the world.Matsutake is not only balanced in nutrition, sufficient, but also is improved that immunity, anticancer be antitumor, treatment diabetes and angiocardiopathy, anti-aging nursing face, promotees the multiple efficacies such as stomach liver protection is dirty.Matsutake is the natural nourishing category of globalization, and industry focuses primarily upon Europe and Japan, because not having more science culture material formula in a balanced way, the artificial large-scale cultivation of therefore loose bacterium is constantly subjected to restrict.
Content of the invention
The technical problem to be solved is to provide a kind of pine bacterium culture material formula, and this culture material formula component is easy to get, comprehensive nutrition.
The technical problem to be solved is achieved by the following technical programs：
A kind of pine bacterium culture material formula, including the raw material of following mass fraction：
Pine sawdust 30 45；
Cotton seed hulls 5 10；
Rape cake 5 10；
Sandy soil 10 20；
Pine needle 5 10；
Wheat Straw 5 10；
Glucose 0.5 1；
Calcium superphosphate 0.2 0.3；
Magnesium sulfate 0.2 0.4；
Described pine sawdust, cotton seed hulls, rape cake, pine needle, Wheat Straw are mixed and pour the particle being ground in pulverizer about 80 mesh into, place into the retting that is soaked in water in fermentation vat, dry after retting to moisture content 10% 15%, mix with sandy soil, glucose, calcium superphosphate, magnesium sulfate again, then, by the mass ratio 5 of compost and solution:1 adds 10-10The mol/L heteroauxin aqueous solution.
Further, the root of described the pine sawdust preferably Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine pulverizes the root wood chip of gained.
Further, described retting process is 10 30 degrees Celsius, 20 50 days；It is preferably 25 degrees Celsius, 30 days.
Preferably, a kind of preparation method of pine bacterium culture material formula as above, including the raw material of following mass fraction：The Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rape cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, described pine sawdust, cotton seed hulls, rape cake, pine needle, Wheat Straw is mixed and pours the particle being ground into 60 100 mesh in pulverizer into, under 20 25 degrees celsius, put into the retting 30 days of being soaked in water in fermentation vat, dry after retting to moisture content 10% 15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, mass ratio 5 by compost and solution:1 adds 10-10The mol/L heteroauxin aqueous solution.
The present invention has advantages below：
1st, this loose bacterium culture material formula is balanced in nutrition, meets the growth demand of loose bacterium comprehensively fully, can accelerate loose bacteria growing speed, shorten growth cycle, expands economic benefit.
2nd, the raw material in this loose bacterium culture material formula is cheap and easy to get, and collocation method is simply it is easy to operate.
3rd, this culture material formula, is used using the collocation that natural material adds chemical replenishers, under the premise of comprehensive nutrition is sufficient, has taken into full account the natural growing environment of loose bacterium so that this compost pine preferably bacteria growing.
4th, in this compost configuration process, the natural ingredients such as pine sawdust, cotton seed hulls, pine needle are pulverized, places into the retting that is soaked in water in fermentation vat so that compost is more easy to be absorbed by loose bacterium.
Specific embodiment
With reference to specific embodiment, it is further elucidated with the present invention.
A kind of pine bacterium culture material formula, the mass fraction proportioning of preferably various components is：The Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rape cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, described pine sawdust, cotton seed hulls, rape cake, pine needle, Wheat Straw is mixed and pours the particle being ground in pulverizer about 80 mesh into, under 20 25 degrees celsius, put into the retting 30 days of being soaked in water in fermentation vat, dry after retting to moisture content 10% 15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, mass ratio 5 by compost and solution:1 adds 10-10The mol/L heteroauxin aqueous solution.
Embodiment described above only have expressed embodiments of the present invention; its description is more concrete and detailed; but therefore can not be interpreted as the restriction to the scope of the claims of the present invention; in every case the technical scheme being obtained in the form of equivalent or equivalent transformation, all should fall within the scope and spirit of the invention.
Claims (2)
1. a kind of pine bacterium compost, is characterized in that the raw material including following mass fraction：
The Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip 25 35；
Cotton seed hulls 5 10；
Rape cake 5 10；
Sandy soil 10 20；
Pine needle 5 10；
Wheat Straw 5 10；
Glucose 0.5 1；
Calcium superphosphate 0.2 0.3；
Magnesium sulfate 0.2 0.4；
The described Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip, cotton seed hulls, rape cake, pine needle, Wheat Straw are mixed and pour the particle being ground into 60 100 mesh in pulverizer into, place into the retting that is soaked in water in fermentation vat, dry after retting to moisture content 10% 15%, mix with sandy soil, glucose, calcium superphosphate, magnesium sulfate again, then, 5 are compared by compost with solution quality:1 adds 10-10The mol/L heteroauxin aqueous solution；
Wherein said retting process is 20 25 degrees Celsius, 30 days.
2. a kind of preparation method of pine bacterium compost as claimed in claim 1 is it is characterised in that described loose bacterium culture material formula is preferably by mass fraction：The Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip 30, cotton seed hulls 8, rape cake 7, sandy soil 15, pine needle 10, Wheat Straw 7, glucose 0.7, calcium superphosphate 0.3, magnesium sulfate 0.3, the described Japanese red pine of more than the 20 years age of trees and/or siberian dwarf pine root wood chip, cotton seed hulls, rape cake, pine needle, Wheat Straw is mixed and pours the particle being ground into 60 100 mesh in pulverizer into, under 25 degrees celsius, put into the retting 30 days of being soaked in water in fermentation vat, dry after retting to moisture content 10% 15%, again with sandy soil, glucose, calcium superphosphate, magnesium sulfate mixes, then, mass ratio 5 by compost and solution:1 adds 10-10The mol/L heteroauxin aqueous solution.
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| CN201410431909.2A CN104193519B (en) | 2014-08-29 | 2014-08-29 | Formula of culture medium for Tricholoma matsutake and preparation method of culture medium |
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| CN201410431909.2A CN104193519B (en) | 2014-08-29 | 2014-08-29 | Formula of culture medium for Tricholoma matsutake and preparation method of culture medium |
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| CN104193519B true CN104193519B (en) | 2017-02-08 |
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| CN106866205A (en) * | 2017-01-24 | 2017-06-20 | 中国科学院昆明植物研究所 | A kind of stropharia rugoso-annulata plantation matrix and preparation method thereof |
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| KR100294794B1 (en) * | 1999-02-04 | 2001-07-03 | 장현유 | A composition of non-sterilized media and an effective cultivation method of mushroom using the same |
| KR100420782B1 (en) * | 2001-11-23 | 2004-03-02 | 삼해상사주식회사 | The culture method of songi mushrooms hypha |
| KR100430412B1 (en) * | 2001-12-27 | 2004-05-04 | 김혁 | Media for cultivating mushroom |
| CN100490630C (en) * | 2006-06-05 | 2009-05-27 | 蒋中海 | Method for cultivation of jiangzhonghai-China |
| CN102815986A (en) * | 2011-06-08 | 2012-12-12 | 王桂芳 | Method for preparing matsutake nutrient |
| CN103467170A (en) * | 2013-08-12 | 2013-12-25 | 凤台县星展食用菌有限公司 | Tricholoma matsutake cultivation medium and preparation method thereof |
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Inventor after: Ma Jianxiong Inventor after: Ma Jianhua Inventor before: Xu Weiming |
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Effective date of registration: 20160901 Address after: The three group of 221200 Jiangsu County town two village Yao Nanjing Osprey Applicant after: JIANGSU HONGSHENG EDIBLE MUSHROOMS Co.,Ltd. Address before: 516800, No. 16, South Road, Longmen County, Huizhou, Guangdong Applicant before: Xu Weiming |
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Address after: 221200 Group 3, Erbao Village, Yaoji Town, Juning County, Jiangsu Province Patentee after: Jiangsu Hongsheng Biotechnology Co.,Ltd. Address before: 221200 Group 3, Erbao Village, Yaoji Town, Juning County, Jiangsu Province Patentee before: JIANGSU HONGSHENG EDIBLE MUSHROOMS Co.,Ltd. |